目的:通过对人受体相关蛋白(receptor accessory protein 6,REEP6)在睾丸和精子中表达研究来探讨REEP6蛋白在雄性生殖中潜在的功能。方法:Western blot检测REEP6在人睾丸及精子中的表达;免疫组化及组织免疫荧光方法对REEP6在人睾丸组织...目的:通过对人受体相关蛋白(receptor accessory protein 6,REEP6)在睾丸和精子中表达研究来探讨REEP6蛋白在雄性生殖中潜在的功能。方法:Western blot检测REEP6在人睾丸及精子中的表达;免疫组化及组织免疫荧光方法对REEP6在人睾丸组织中的定位进行研究;人精子免疫荧光对REEP6在精子顶体反应前后的定位进行研究。结果:REEP6在人睾丸及精子中均有表达,在睾丸组织中REEP6定位于各级生精细胞的细胞膜,在精子中则定位于核后区附近,并且在顶体反应前后,REEP6在人精子的定位及表达量不发生改变。结论:REEP6在人睾丸和精子中均有表达,其表达定位具有细胞特异性和区域特异性,提示其可能在人睾丸精子发生和(或)精卵融合过程中发挥作用。展开更多
While degenerative diseases of the central nervous system are commonly linked to age-related macular degeneration and glaucoma,they have also been infrequently associated with retinitis pigmentosa,a condition defined ...While degenerative diseases of the central nervous system are commonly linked to age-related macular degeneration and glaucoma,they have also been infrequently associated with retinitis pigmentosa,a condition defined by retinal degeneration that can be caused by an isoform of receptor expression enhancing protein 6(REEP6)expressed in rod photoreceptors.In this study,we used REEP6 knockout mice(REEP6^(-/-))and wild-type mice(REEP6^(+/+))to examine neurodegenerative pathology within the visual pathways and neural activity in the primary visual cortex(V1)at three specific time points(1,6,and 10 months)during retinitis pigmentosa progression.Microglial activation was observed in both the retina and the primary visual cortex starting at 1 month of age,but no such activation was detected in the lateral geniculate nucleus at any time point.Not only was increased microglial activation observed at 6 and 10 months within the primary visual cortex of REEP6^(-/-)mice,but also coinciding with elevated levels of phosphorylated Tau expression.At 6 and 10 months of age,primary visual cortex neurons in REEP6^(-/-)mice exhibited reduced responses to grating stimuli and increased spontaneous activity compared with neurons in the primary visual cortex of mice in the control group.Our findings show that retinitis pigmentosa induces neurodegenerative pathology within the visual pathway of mice,particularly in the primary visual cortex,suggesting that ocular disease contributes substantially to central nervous system degeneration.It may provide new clues for the selection of treatment opportunities and the development of therapeutic measures for the subsequent treatment of retinitis pigmentosa or even other retinal degenerative diseases.展开更多
文摘目的:通过对人受体相关蛋白(receptor accessory protein 6,REEP6)在睾丸和精子中表达研究来探讨REEP6蛋白在雄性生殖中潜在的功能。方法:Western blot检测REEP6在人睾丸及精子中的表达;免疫组化及组织免疫荧光方法对REEP6在人睾丸组织中的定位进行研究;人精子免疫荧光对REEP6在精子顶体反应前后的定位进行研究。结果:REEP6在人睾丸及精子中均有表达,在睾丸组织中REEP6定位于各级生精细胞的细胞膜,在精子中则定位于核后区附近,并且在顶体反应前后,REEP6在人精子的定位及表达量不发生改变。结论:REEP6在人睾丸和精子中均有表达,其表达定位具有细胞特异性和区域特异性,提示其可能在人睾丸精子发生和(或)精卵融合过程中发挥作用。
基金supported by STI 2030-Major Projects,No.2022ZD0208503(to DY)the Fund of Chinese Academy of Science(“Xi Bu Zhi Guang”Project)(to YY)the Sichuan Science and Technology Program,No.2023YFS0312(to YY).
文摘While degenerative diseases of the central nervous system are commonly linked to age-related macular degeneration and glaucoma,they have also been infrequently associated with retinitis pigmentosa,a condition defined by retinal degeneration that can be caused by an isoform of receptor expression enhancing protein 6(REEP6)expressed in rod photoreceptors.In this study,we used REEP6 knockout mice(REEP6^(-/-))and wild-type mice(REEP6^(+/+))to examine neurodegenerative pathology within the visual pathways and neural activity in the primary visual cortex(V1)at three specific time points(1,6,and 10 months)during retinitis pigmentosa progression.Microglial activation was observed in both the retina and the primary visual cortex starting at 1 month of age,but no such activation was detected in the lateral geniculate nucleus at any time point.Not only was increased microglial activation observed at 6 and 10 months within the primary visual cortex of REEP6^(-/-)mice,but also coinciding with elevated levels of phosphorylated Tau expression.At 6 and 10 months of age,primary visual cortex neurons in REEP6^(-/-)mice exhibited reduced responses to grating stimuli and increased spontaneous activity compared with neurons in the primary visual cortex of mice in the control group.Our findings show that retinitis pigmentosa induces neurodegenerative pathology within the visual pathway of mice,particularly in the primary visual cortex,suggesting that ocular disease contributes substantially to central nervous system degeneration.It may provide new clues for the selection of treatment opportunities and the development of therapeutic measures for the subsequent treatment of retinitis pigmentosa or even other retinal degenerative diseases.
