OBJECTIVE:To analyze the chemical components of Mudan Huaban recipe(牡丹化斑方,MHR)and evaluate its efficacy and possible mechanism in melasma mice.METHODS:The chemical compositions of MHR were determined by the ultra...OBJECTIVE:To analyze the chemical components of Mudan Huaban recipe(牡丹化斑方,MHR)and evaluate its efficacy and possible mechanism in melasma mice.METHODS:The chemical compositions of MHR were determined by the ultra-high performance liquid chromatography coupled with quadrupole-exactive mass spectrometry method.Female C57BL/6 mice were exposed to ultraviolet B and progesterone for 21 d to induce melasma,and Fontana-Masson staining was used to assess the effects of MHR on melasma.Luteinizing hormone(LH),estradiol,and follicle-stimulating hormone levels were detected by enzyme-linked immunosorbent assay.The superoxide dismutase(SOD)activity and malonic dialdehyde content were detected by chemiluminescence.Tyrosinase and related proteins expressions were detected by Western blots and immunohistochemistry.RESULTS:A total of 43 chemical components were identified in MHR,MHR significantly decreased the melanin particles of melasma mice.MHR treatment significantly reversed the high contents of LH and low activity of SOD in models.MHR significantly reduced the higher levels of tyrosinase,tyrosinase-related proteins-1(TRP-1),TRP-2,microphthalmia-associated transcription factor and phosphorylation of cyclic adenosine monophosphate response element-binding protein(pCREB)/CREB in the skin of melasma mice.CONCLUSIONS:MHR protects against melasma via regulation of sex hormones,oxidative stress,and melanogenesis-related proteins,suggesting its possible use as a supplement and alternative drug therapy for melasma.展开更多
Objective To investigate the active components and mechanisms by which Qingre Zaoshi Jiedu Recipe influences the transformation from cervical high-risk human papillomavirus(HR-HPV)-related cervicitis to cancer,utilizi...Objective To investigate the active components and mechanisms by which Qingre Zaoshi Jiedu Recipe influences the transformation from cervical high-risk human papillomavirus(HR-HPV)-related cervicitis to cancer,utilizing network pharmacology,Gene Expression Omnibus(GEO)data,and molecular docking techniques.Methods Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),HERB,and the Encyclopedia of Traditional Chinese Medicine(ETCM)databases were used to screen out the active ingredients and related targets of Qingre Zaoshi Jiedu Recipe.The GeneCards database for disease targets at different stages of HR-HPV-related cervicitis–cancer were searched.The String platform to construct a protein-protein interaction network and identify key targets was utilized.Enrichment analysis of intersecting genes was performed using the DAVID database.The GSE149763 dataset from the GEO to identify differential genes involved in the transformation from cervicitis to cervical cancer by comparing cervicitis with cervical intraepithelial neoplasia III and cervical cancer was utilized.R language to generate volcano plots,heat maps,and key target expression trend charts were employed.Molecular docking of key pathway targets and main compounds of Qingre Zaoshi Jiedu Recipe for HR-HPV-related cervicitis–cancer was performed using AutoDock Vina.Results The study identified 185 main active ingredients of Qingre Zaoshi Jiedu Recipe.The protein–protein interaction network indicates that the core targets for interfering with HR-HPV-related inflammation–cancer transformation include TNF,IL-6,IL-1β,CXCL8,IL-1α,IFN-γ,IL-10,CCL2,CCL5,and CXCL10.KEGG pathway analysis indicates that Qingre Zaoshi Jiedu Recipe primarily affects HR-HPV-related inflammation–cancer transformation via the Toll-like receptor signaling pathway.GEO analysis identified the Toll-like receptor pathway as crucial across various stages of cervicitis–cancer lesions,with CXCL10 emerging as a key target.Molecular docking analysis revealed that the primary components of Qingre Zaoshi Jiedu Recipe effectively bind to TLR4.Conclusion Qingre Zaoshi Jiedu Recipe can interfere with HR-HPV-related cervicitis–cancer transformation by acting on TLR4 through the Toll-like receptor pathway.展开更多
OBJECTIVES:To investigate the effect of Bushen Tongluo recipe(BSTLR, 补肾通络方) on rats with diabetic kidney disease(DKD) and to explore the underlying mechanism of action. METHODS:The rat model of DKD was establishe...OBJECTIVES:To investigate the effect of Bushen Tongluo recipe(BSTLR, 补肾通络方) on rats with diabetic kidney disease(DKD) and to explore the underlying mechanism of action. METHODS:The rat model of DKD was established, and rats were treated with different doses of BSTLR. Body weight and the levels of urinary protein, α1-microglobulin, glucose, blood urea nitrogen, creatinine, Cystatin C, superoxide dismutase, malondialdehyde, and catalase were analyzed biochemically or by enzyme-linked immunosorbent assay. The pathological damage to renal tissues was assessed by hematoxylin-eosin staining. Immunohistochemical staining was carried out to detect the expression levels of fibronectin, E-cadherin, α-smooth muscle actin, laminin, vimentin, collagen type Ⅳ in kidney tissues. Western blot analysis was conducted to analyze the expression levels of Nephrin, Desmin, Podocin, transforming growth factor-β1, mothers against decapentaplegic homolog 3(Smad3), Notch1, jagged, hairy and enhancer of split 1(Hes1) in kidney tissues, and the expression levels of maternally expressed gene 3(MEG3) and mi R-145 were measured by quantitative reverse transcription-polymerase chain reaction. Moreover, dual-luciferase reporter assay was employed to verify the binding of mi R-145 to MEG3. RESULTS:BSTLR increased the body weight of DKD rats, effectively ameliorated the renal function and pathological injury in DKD, regulated the balance of renal oxidative stress, inhibited the TGF/Notch signaling pathway, and affected the variations in the lnc RNA MEG3/mi R-145 axis. CONCLUSION:BSTLR improved oxidative stress homeostasis, inhibited the TGF/Notch signaling pathway, and regulated the lnc RNA MEG3/mi R-145 axis, effectively delaying the progression of DKD.展开更多
Background:Hypertension,a prevalent disease,is a significant risk factor for coronary heart disease.Huoxue Qianyang Qutan Recipe (HQQR),a traditional Chinese herbal remedy,has been used for treating hypertension over ...Background:Hypertension,a prevalent disease,is a significant risk factor for coronary heart disease.Huoxue Qianyang Qutan Recipe (HQQR),a traditional Chinese herbal remedy,has been used for treating hypertension over several years.Objective:This study assesses HQQR’s efficacy for controlling blood pressure among patients with hypertension related to blood stasis,yang hyperactivity and phlegm.Design,setting,participants and interventions:A randomized controlled trial was conducted at the Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,China,from July 2020 to June 2022.Major components of HQQR were identified using thin-layer chromatography and high-performance liquid chromatography.Participants aged18–80 years,exhibiting traditional Chinese medicine syndromes of blood stasis,yang hyperactivity or phlegm,along with grades 1 or 2 hypertension,were randomly categorized into two groups.The intervention group was given HQQR granules alongside conventional hypertension treatment,while the control group was given placebo granules in addition to conventional treatment for 12 weeks.Main outcome measures:The primary outcome was clinic blood pressure,whereas secondary outcomes included metabolic indices (e.g.,homeostasis model assessment of insulin resistance[HOMA-IR],total cholesterol[TC],low-density lipoprotein cholesterol and triglyceride),target organ damage indices (left ventricular mass index and urinary albumin creatinine ratio[UACR]) and inflammation indices(interleukin-6[IL-6]and high-sensitivity C-reactive protein[hs-CRP]).Results:HQQR’s primary components were identified as salvianolic acid B,emodin and ferulic acid.Of the 216 participants (108 in each group),compared to the control,the intervention group exhibited significant improvements (P<0.001) in clinic systolic blood pressure ([136.24±7.63]vs[130.06±8.50]mmHg),clinic diastolic blood pressure ([84.34±8.72]vs[80.46±6.05]mmHg),home systolic blood pressure([131.64±8.74]vs[122.36±8.45]mmHg) and home diastolic blood pressure ([78.47±9.53]vs[71.79±6.82]mm Hg).HQQR demonstrated a reduction in ambulatory blood pressure (24-hour systolic blood pressure:[133.75±10.49]vs[132.46±8.84]mmHg and 24-hour diastolic blood pressure:[84.12±8.01]vs[82.11±7.45]mmHg) and an improvement in HOMA-IR ([4.09±1.72]vs[3.98±1.44]),TC ([4.66±1.47]vs[3.75±1.81]mmol/L) and UACR (75.94[5.12,401.12]vs 45.61[4.26,234.26]).Moreover,HQQR demonstrated a decrease in hs-CRP (1.46[0.10,10.53]vs 0.57[0.12,3.99]mg/L) and IL-6 (6.69[2.00,29.74]vs 5.27[2.00,9.73]pg/mL),with no reported side effects (P<0.001).Conclusion:This study highlights the therapeutic potential of HQQR use in ameliorating blood pressure,glycolipid metabolism,and inflammation in patients with hypertension.展开更多
OBJECTIVE:To study whether Shenshuai recipe(肾衰方,SSR)can play a protective role on chronic kidney disease myocardial injury model through phosphatase and tensin homolog-induced putative kinase 1(PINK1)/E3 ubiquitin ...OBJECTIVE:To study whether Shenshuai recipe(肾衰方,SSR)can play a protective role on chronic kidney disease myocardial injury model through phosphatase and tensin homolog-induced putative kinase 1(PINK1)/E3 ubiquitin ligase Parkin(Parkin)mitochondrial autophagy pathway.METHODS:Forty-eight nephrectomized rats were randomly divided into six groups:sham-operated group,model group,Benazepril group,low,medium and highdose groups of SSR.The rats were given the corresponding intervention for six weeks,then were sacrificed.Serum was examined by enzyme linked immunosorbent assay(ELISA).Cardiac ultrasound was used to detect cardiac function in 5/6 nephrectomized rats.Myocardial tissue was examined by light and electron microscopy;PINK1,Parkin,microtubule-associated protein 1 light chain 3 II(LC3B),sequestosome 1(P62),BECN1(Beclin-1)and dynamin-related protein 1(Drp-1)were measured by real time polymerase chain reaction(RT-PCR),Western blot(WB)and immunohistochemistry(IHC).RESULTS:The expression levels of blood urea nitrogen(BUN)and creatinine(SCr)in the model group were significantly higher than those in the sham-operated group,indicating that modeling was successful.SSR can protect myocardium by reducing the relative expression of creatine kinase myocardial isoenzyme and hypersensitivity cardiac troponin I(P<0.05).SSR can improve cardiac function in rats after ultrasound testing.SSR can improve the pathological manifestations of myocardial tissue after Masson staining.SSR can increase the number of autophagosomes and autophagiclysosomes in 5/6 nephrectomized rats(P<0.05).Determined by RT-PCR,WB and IHC,SSR can increase the relative expression of PINK1,Parkin,and LC3B(P<0.05),and decrease the relative expression of P62,Beclin-1 and Drp-1(P<0.05).CONCLUSIONS:The PINK1/Parkin mitochondrial autophagy pathway in myocardial tissues in 5/6 nephrectomy CKD myocardial injury rats was inhibited.SSR can activate PINK1/Parkin mitochondrial autophagy to enhance mitochondrial autophagy,and play a protective role in myocardial tissues.展开更多
OBJECTIVE:To elucidate the mechanism by which Huoxue Jiedu Huayu recipe(活血解毒化瘀方,HJHR)regulates angiogenesis in the contralateral kidney of unilateral ureteral obstruction(UUO)rats and the mechanism by which it ...OBJECTIVE:To elucidate the mechanism by which Huoxue Jiedu Huayu recipe(活血解毒化瘀方,HJHR)regulates angiogenesis in the contralateral kidney of unilateral ureteral obstruction(UUO)rats and the mechanism by which it reduces of renal fibrosis.METHODS:Male Wistar rats were randomly divided into 4 groups:the sham group,UUO group(180 d of left ureter ligation),UUO plus eplerenone(EPL)group,and UUO plus HJHR group.After 180 d of oral drug administration,blood and contralateral kidneys were collected for analysis.Angiogenesis-and fibrosis-related indexes were detected.RESULTS:HJHR and EPL improved structural damage and renal interstitial fibrosis in the contralateral kidney and reduced the protein expression levels ofα-smooth muscle actin(α-SMA),vimentin and collagen I.Moreover,these treatments could reduce the expression of vascular endothelial growth factor-A(VEGFA)by inhibiting the infiltration of macrophages.Furthermore,HJHR and EPL significantly reduced the expression of CD34 and CD105 by downregulating VEGFA production,which inhibited angiogenesis.Finally,the coexpressions of CD34,CD105 andα-SMA were decreased in the HJHR and EPL groups,indicating that endothelial-to-mesenchymal transition was inhibited.CONCLUSIONS:These findings confirm that HJHR alleviates contralateral renal fibrosis by inhibiting VEGFAinduced angiogenesis,encourage the use of HJHR against renal interstitial fibrosis and provide a theoretical basis for the clinical management of patients with CKD.展开更多
BACKGROUND Chronic heart failure is a complex clinical syndrome.The Chinese herbal compound preparation Jianpi Huatan Quyu recipe has been used to treat chronic heart failure;however,the underlying molecular mechanism...BACKGROUND Chronic heart failure is a complex clinical syndrome.The Chinese herbal compound preparation Jianpi Huatan Quyu recipe has been used to treat chronic heart failure;however,the underlying molecular mechanism is still not clear.AIM To identify the effective active ingredients of Jianpi Huatan Quyu recipe and explore its molecular mechanism in the treatment of chronic heart failure.METHODS The effective active ingredients of eight herbs composing Jianpi Huatan Quyu recipe were identified using the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform.The target genes of chronic heart failure were searched in the Genecards database.The target proteins of active ingredients were mapped to chronic heart failure target genes to obtain the common drugdisease targets,which were then used to construct a key chemical componenttarget network using Cytoscape 3.7.2 software.The protein-protein interaction network was constructed using the String database.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were performed through the Metascape database.Finally,our previously published relevant articles were searched to verify the results obtained via network pharmacology.RESULTS A total of 227 effective active ingredients for Jianpi Huatan Quyu recipe were identified,of which quercetin,kaempferol,7-methoxy-2-methyl isoflavone,formononetin,and isorhamnetin may be key active ingredients and involved in the therapeutic effects of TCM by acting on STAT3,MAPK3,AKT1,JUN,MAPK1,TP53,TNF,HSP90AA1,p65,MAPK8,MAPK14,IL6,EGFR,EDN1,FOS,and other proteins.The pathways identified by KEGG enrichment analysis include pathways in cancer,IL-17 signaling pathway,PI3K-Akt signaling pathway,HIF-1 signaling pathway,calcium signaling pathway,cAMP signaling pathway,NF-kappaB signaling pathway,AMPK signaling pathway,etc.Previous studies on Jianpi Huatan Quyu recipe suggested that this Chinese compound preparation can regulate the TNF-α,IL-6,MAPK,cAMP,and AMPK pathways to affect the mitochondrial structure of myocardial cells,oxidative stress,and energy metabolism,thus achieving the therapeutic effects on chronic heart failure.CONCLUSION The Chinese medicine compound preparation Jianpi Huatan Quyu recipe exerts therapeutic effects on chronic heart failure possibly by influencing the mitochondrial structure of cardiomyocytes,oxidative stress,energy metabolism,and other processes.Future studies are warranted to investigate the role of the IL-17 signaling pathway,PI3K-Akt signaling pathway,HIF-1 signaling pathway,and other pathways in mediating the therapeutic effects of Jianpi Huatan Quyu recipe on chronic heart failure.展开更多
Objective:Tumor-derived exosomes(TDEs)play crucial roles in intercellular communication.Hypoxia in the tumor microenvironment enhances secretion of TDEs and accelerates tumor metastasis.Jiedu recipe(JR),a traditional ...Objective:Tumor-derived exosomes(TDEs)play crucial roles in intercellular communication.Hypoxia in the tumor microenvironment enhances secretion of TDEs and accelerates tumor metastasis.Jiedu recipe(JR),a traditional Chinese medicinal formula,has demonstrated efficacy in preventing the metastasis of hepatocellular carcinoma(HCC).However,the underlying mechanism remains largely unknown.Methods:Animal experiments were performed to investigate the metastasis-preventing effects of JR.Bioinformatics analysis and in vitro assays were conducted to explore the potential targets and active components of JR.TDEs were assessed using nanoparticle tracking analysis(NTA)and Western blotting(WB).Exosomes derived from normoxic or hypoxic HCC cells(H-TDEs)were collected to establish premetastatic mouse models.JR was intragastrically administered to evaluate its metastasis-preventive effects.WB and lysosomal staining were performed to investigate the effects of JR on lysosomal function and autophagy.Bioinformatics analysis,WB,NTA,and immunofluorescence staining were used to identify the active components and potential targets of JR.Results:JR effectively inhibited subcutaneous-tumor-promoted lung premetastatic niche development and tumor metastasis.It inhibited the release of exosomes from tumor cells under hypoxic condition.JR treatment promoted both lysosomal acidification and suppressed secretory autophagy,which were dysregulated in hypoxic tumor cells.Quercetin was identified as the active component in JR,and the epidermal growth factor receptor(EGFR)was identified as a potential target.Quercetin inhibited EGFR phosphorylation and promoted the nuclear translocation of transcription factor EB(TFEB).Hypoxia-impaired lysosomal function was restored,and secretory autophagy was alleviated by quercetin treatment.Conclusion:JR suppressed HCC metastasis by inhibiting hypoxia-stimulated exosome release,restoring lysosomal function,and suppressing secretory autophagy.Quercetin acted as a key component of JR and regulated TDE release through EGFR-TFEB signaling.Our study provides a potential strategy for retarding tumor metastasis by targeting H-TDE secretion.展开更多
OBJECTIVE:To investigate the efficacy of Longteng Tongluo recipe(龙藤通络方,LTTL)combined with threestep analgesia for the treatment of lung cancer pain,and the changes in serum miRNA expressions before-and after trea...OBJECTIVE:To investigate the efficacy of Longteng Tongluo recipe(龙藤通络方,LTTL)combined with threestep analgesia for the treatment of lung cancer pain,and the changes in serum miRNA expressions before-and after treatment with LTTL and its correlation with lung cancer pain.The possible mechanism underlying LTTL effects on the treatment of lung cancer pain was conducted.METHODS:The pilot study was conducted at the oncology ward of the Yueyang Hospital and the Longhua Hospital between March 2018 and October 2019.A prospective,single-blind,placebo controlled,randomized clinical trial of LTTL or placebo combined with three-step analgesia treatments were administered to 24 cancer pain patients diagnosed with lung cancer.Analgesic efficacy was investigated as the primary outcome.Equivalent morphine consumption and numerical rating scale(NRS)scores were used as the secondary outcome.In the present study,we utilized deep sequencing techniques to compare the differential miRNA expressions in serum samples obtained from two groups:the lung cancer pain treatment group(LTTL+three-step analgesia)and the control group(placebo+three-step analgesia).Next,we employed the target prediction database to investigate the target genes for differential miRNA expressions and Gene Ontology(GO)analysis along with Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis to examine the roles and the major biochemical and signaling pathways related to the differentially expressed target genes,respectively.RESULTS:LTTL treatment significantly reduces the NRS score(P=0.021)as compared to those before treatment,along with significant reductions in the total morphine equivalent consumption(P=0.007)and the average daily equivalent morphine consumption(P=0.003)as opposed to the control group.The expressions of 31 miRNAs differed considerably between the two groups of patients(≥2 times up-modulated or down-regulated between these groups,P<0.05).For instance,the miRNAs expression levels for patients before treatment(has-miR-2110 and has-miR-7d-3p)were significantly enhanced as compared to the healthy people,after LTTL treatment,the expressions of miR-2110 and miR-7d-3p in patients with lung cancer pain reduced significantly.Studies show that the above two miRNAs were significantly associated with lung cancer pain,which could mediate lung cancer pain.Furthermore,we identified 355 genes as potential targets of the 31 differentially expressed miRNAs.Pathway enrichment analyses using KEGG and GO analysis indicated that these target genes may play a crucial role in the development and modulation of lung cancer pain.CONCLUSION:LTTL demonstrated a discernible impact on alleviating lung cancer pain and its mechanism of action may be related to the downregulation of has-miR-2110 and has-miR-7d-3p expressions.This pilot study provides support for further exploration of LTTL in patients with lung cancer pain.展开更多
OBJECTIVE: To evaluate the clinical efficacy and safety of Shenzhu Guanxin recipe granules(参术冠心颗粒, SGR) in treating patients with intermediate coronary lesions(ICL), and to investigate the potential mechanism th...OBJECTIVE: To evaluate the clinical efficacy and safety of Shenzhu Guanxin recipe granules(参术冠心颗粒, SGR) in treating patients with intermediate coronary lesions(ICL), and to investigate the potential mechanism though a transcriptome sequencing approach. METHODS: ICL patients with Qi deficiency and phlegm stasis were adopted and randomly assigned to a case group or a control by random number generator in a 1∶1 randomization ratio to evaluate the clinical efficacy. RESULTS: There was no significant difference between the two groups in coronary computed tomography angiography related indexes in the two groups before and after intervention. Through the gene chip expression analysis, it is finally concluded that there are 355 differential mRNAs(190 up-regulated genes and 165 down regulated genes) when compared the SGR group and placebo group. Through protein-protein interaction network analysis of differentially expressed genes, 10 hub genes were finally obtained: CACNA2D2, CACNA2D3, DNAJC6, FGF12, SGSM2, CACNA1G, LRP6, KIF25, OXTR, UPB1. CONCLUSIONS: SGR combined with Western Medicine can be safely used to treat ICL patients with Qi deficiency and phlegm stasis. The possible mechanism of action and relevant gene loci and pathway were proposed.展开更多
Background:This study aims to explore the therapeutic effects of the Qi Teng Mai Ning recipe on ischemic stroke and vascular cognitive impairment through its potential to modulate cellular autophagy,with a focus on id...Background:This study aims to explore the therapeutic effects of the Qi Teng Mai Ning recipe on ischemic stroke and vascular cognitive impairment through its potential to modulate cellular autophagy,with a focus on identifying its active ingredients and their target proteins.Methods:The study began with the identification of active ingredients in the Qi Teng Mai Ning recipe.It proceeded to screen the gene expression omnibus database for ischemic stroke and vascular cognitive impairment-associated differentially expressed mRNAs and to identify cellular autophagy-related proteins via the Human Autophagy Database.These proteins were annotated with Gene Ontology and Kyoto Encyclopedia of Genes and Genomes functions and subjected to molecular docking with the recipe’s core active ingredients.In vitro cell experiments were conducted on hypoxic HT22 cells,involving CCK8 assay,lentiviral transfection to silence autophagy related 9B(ATG9B),immunofluorescence staining,and qPCR validation to investigate the effects of the recipe on autophagy.Results:The analysis identified 104 active ingredients targeting 408 proteins and forming a complex ingredient-target network.Intersecting 55 ischemic stroke-related and 909 vascular cognitive impairment-related differentially expressed mRNAs revealed 14 co-expressed mRNAs.Molecular docking showed quercetin,kaempferol,myrcene,and conferone as key ingredients targeting autophagy-related proteins.Cellular experiments indicated that the recipe significantly enhanced cell viability under hypoxic conditions,reduced apoptosis,and modulated the expression of autophagy-related factors,thereby decreasing apoptosis rates in HT22 cells.Conclusion:The Qi Teng Mai Ning recipe offers protective effects against ischemic stroke and vascular cognitive impairment by modulating autophagy-related proteins.Its efficacy highlights the potential of traditional Chinese medicine in treating these conditions,though further research is needed to fully understand its mechanisms and clinical applications.展开更多
Objective:To observe the effect and possible mechanism of action of Bushen Bitong recipe(BSBT)containing serum on IL-1β-induced chondrocyte apoptosis.Methods:Generation 3 rat chondrocytes were randomized into Control...Objective:To observe the effect and possible mechanism of action of Bushen Bitong recipe(BSBT)containing serum on IL-1β-induced chondrocyte apoptosis.Methods:Generation 3 rat chondrocytes were randomized into Control,IL-1β,IL-1β+BSBT(L),IL-1β+BSBT(M),and IL-1β+BSBT(H)groups(5%,10%and 15%BSBT-containing serum),and then 24h after intervention respectively,the cell proliferation and Apoptosis rate;Western blot detected the expression levels of Bcl-2,BAX,Caspase-3,SOX9,NF-κB p65,MMP-13 proteins in chondrocytes.ELISA detected the levels of TNF-α,IL-6,and bFGF in the supernatants of chondrocyte culture.Results:Compared with Control group,cell proliferation activity decreased,apoptosis rate increased,NF-κB p65,MMP-13 protein level and TNF-α,IL-6 level increased,and SOX9 protein level and bFGF level decreased in IL-1βgroup;compared with IL-1βgroup,different concentrations of BSBT-containing serum group,cell proliferation activity increased,and apoptosis rate decreased.NF-κB p65,MMP-13 protein level and TNF-α,IL-6 level decreased,SOX9 protein level and bFGF level increased;compared with IL-1β+BSBT(L)group,cell proliferation activity increased,apoptosis rate decreased in IL-1β+BSBT(M)and IL-1β+BSBT(H)groups,and NF-κB p65,MMP-13 protein level and TNF-αlevel decreased.13 protein levels and TNF-αand IL-6 levels decreased,and SOX9 protein levels and bFGF levels increased.Conclusion:BSBT-containing serum may promote IL-1β-induced proliferation of chondrocytes,reduce apoptosis,improve the microenvironment of chondrocytes,and promote cartilage repair through the SOX9/NF-κB/MMP-13 signaling pathway.展开更多
Chronic viral hepatitis B (HBV) remains a major public health problem in Burkina Faso. Since access to diagnostic tests and treatments is limited because of their high cost, the majority of the population turn to trad...Chronic viral hepatitis B (HBV) remains a major public health problem in Burkina Faso. Since access to diagnostic tests and treatments is limited because of their high cost, the majority of the population turn to traditional herbal treatments. This study aimed to evaluate the effectiveness of a plant recipe called Hepatib tiben. It consisted of comparing certain biochemical and molecular parameters of patients infected with HBV that were supported by the recipe. The patients were recruited in Ouagadougou by the traditional health practitioner according to the requirements of the study. Thus 44 patients aged 20 to 61 years and carrier of HBsAg for at least 06 months were treated with Hepatib tiben. The tests were performed in the laboratory before and three months after the treatment. ELISA tests were used to confirm the presence of HBsAg and search for anti-HCV antibodies;transaminases, creatinine were quantified by the “Chem 400” automaton and the viral load of HBV by Real-time PCR. The analysis of the results reveals an improvement of the biochemical and molecular parameters of the patients with the following means (ASAT: 21.02 ± 9.97;ALAT: 21.11 ± 13.27;DNA: 1571.82 ± 3990.97 with p = 0.01 for each). As for HBsAg, its disappearance was observed in 4.55% of patients after treatment. The evaluation of the creatinine parameter explained that the recipe of plants has a tolerated effect on the kidneys of treated patients. These results, while encouraging, need to be complemented by further research for the development of effective phytomedicine to treat and eliminate this viral hepatitis B virus.展开更多
[Objectives]To observe the therapeutic effect of intragastric administration of Jiangtang Shuxin recipe on diabetic heart failure(DHF)in rats and to explore its mechanism.[Methods]Fifty SD rats were randomly divided i...[Objectives]To observe the therapeutic effect of intragastric administration of Jiangtang Shuxin recipe on diabetic heart failure(DHF)in rats and to explore its mechanism.[Methods]Fifty SD rats were randomly divided into five groups,with 10 rats in each group.DHF models were prepared in the low-dose group,high-dose group,Western medicine group,and model group except the control group.Rats in the low-dose and high-dose groups were given 1.0 and 1.5 g/(kg·d)Jiangtang Shuxin recipe suspension by gavage,respectively.Rats in the Western medicine group were given gliquidone and benazepril by gavage for 2 months,and were fed with high-fat diet.Rats in the control group were fed with ordinary diet.Fasting blood glucose(FBG),serum triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),C-reactive protein(CRP),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),creatine kinase isoenzyme(CK-MB),lactate dehydrogenase(LDH),pathological morphology of myocardial tissue,NF-κB p65 protein and IκBαprotein were compared among groups.[Results]Compared with the control group,the levels of FBG,serum TG,LDL-C,CRP,IL-6,TNF-α,CK-MB and LDH increased,while the level of serum HDL-C decreased.The myocardial tissue was seriously damaged,and the expression of NF-κB p65 protein increased,while the expression of IκBαprotein decreased in the other four gruops(all P<0.05).Compared with the model group,the levels of FBG,serum TG,LDL-C,CRP,IL-6,TNF-α,CK-MB and LDH decreased,while the serum HDL-C level increased.The myocardial tissue damage was alleviated,and the expression of NF-κB p65 protein decreased,while the expression of IκBαprotein increased in the low-dose group,high-dose group and Western medicine group(all P<0.05).Compared with the Western medicine group,the levels of FBG,serum TG,LDL-C,CRP,IL-6,TNF-α,CK-MB and LDH decreased,and the level of serum HDL-C increased in the high-dose group(all P<0.05).[Conclusions]Jiangtang Shuxin recipe has a therapeutic effect on DHF in rats,with the best effect in the high-dose group.It can not only alleviate high glucose and high fat state,but also reduce myocardial injury and inflammation,and improve the pathological morphology of myocardial cells.The mechanism may be related to its inhibition of NF-кB signaling pathway.展开更多
The consumption of a mixture of leafy vegetables has always been a practice anchored in the dietary habits of the people of Brazzaville. The objective of this study was to evaluate the acute and subacute toxicities of...The consumption of a mixture of leafy vegetables has always been a practice anchored in the dietary habits of the people of Brazzaville. The objective of this study was to evaluate the acute and subacute toxicities of the aqueous extract of the recipe based on three leafy vegetables (Amaranthus hybridus L., Spinacia oleracea, Brassica campestris L.) in laboratory rodents. The aqueous extract of the food recipe was administered orally using a cannula. Regarding acute toxicity, the administration of single doses of 5000 and 10,000 mg/kg to mice had no significant effects on behavior. On the other hand, weight loss was significantly (**p < 0.01) compared to control mice (1 mL/100g). The results on subacute toxicity showed that daily administration of the aqueous extract of the food recipe at a dose of 1000 mg/kg for 21 days in rats resulted in a non-significant weight gain, and caused changes in some biochemical parameters including HDL-cholesterol levels, blood sugar levels and ALAT activity.展开更多
BACKGROUND Traditional Chinese medicine(TCM)is widely used as an important complementary and alternative healthcare system for cancer treatment in Asian countries.Network pharmacology,which utilizes various database p...BACKGROUND Traditional Chinese medicine(TCM)is widely used as an important complementary and alternative healthcare system for cancer treatment in Asian countries.Network pharmacology,which utilizes various database platforms and computer software to study the interactions between complex drug components in vivo,is particularly useful for studying the pharmacodynamic mechanisms of multi-pathway and multi-target Chinese medicines.AIM To explore the potential targets and function of Jianpi Yiwei Recipe treatment of gastric cancer(GC)through network pharmacology and molecular docking.METHODS Data on the components of Jianpi Yiwei Recipe(Radix Astragali,Radix Codonopsis,Agrimonia eupatoria,Atractylodes macrocephala Koidz.,Poria cocos,stirbaked rhizoma dioscoreae,Amomum villosum Lour.,fried Fructus Aurantii,pericarpium citri reticulatae,Rhizoma Pinelliae Preparata,and Radix Glycyrrhizae Preparata)were collected and screened by using the TCM systems pharmacology database and analysis platform(TCMSP).Then the targets of these compounds were predicted.GC-related targets were screened using the GeneCards database.Venn diagram was used to identify common targets.An active ingredient-core target interaction network and a protein-protein interaction(PPI)network were built.Moreover,we performed gene ontology(GO)functional annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses on the core targets and validated them by molecular docking.RESULTS TCMSP screening revealed 11 active components and 184 targets,whereas GeneCards found 10118 disease-related targets,with 180 shared targets between them.Topology analysis of the PPI network identified 38 targets,including ATK1,TP53,and tumor necrosis factor,as key targets for the treatment of GC by Jianpi Yiwei Recipe.Quercetin,naringenin,luteolin,etc.,may be the main active components of Jianpi Yiwei Recipe.GO enrichment analysis identified 2809,1218,and 553 functions related to biological process,molecular function,and cellular component,respectively.KEGG pathway enrichment analysis revealed 167 related pathways,mainly involved in cancer,endocrine resistance,and AGE-RAGE signaling in diabetic complication.Validation with molecular docking analysis showed docking of key active components with core targets.CONCLUSION Jianpi Yiwei Recipe plays a therapeutic role in GC through multiple components,targets,and pathways.These findings form a basis for follow-up exploration of Jianpi Yiwei Recipe in the treatment of GC.展开更多
AIM To propose a hypothesis defining theabsorption,distribution,metabolism andelimination of traditional Chinese recipe（TCR）-component in blood of healthy subjects andpatients,and estimate its correctness.METHODS Th...AIM To propose a hypothesis defining theabsorption,distribution,metabolism andelimination of traditional Chinese recipe（TCR）-component in blood of healthy subjects andpatients,and estimate its correctness.METHODS The pharmacokinetics（PK）of samedose of drug was studied in the animal model oftraditional Chinese syndrome（S）and healthyanimals.The classification,terminology,concept and significance of the hypothesis wereset forth with evidence provided in the presentstudy.The hypotheses consisted of traditionalChinese syndrome PK（S-PK）and traditionalChinese recipe PK（R-PK）.Firstly,the observedtetramethylpyrazine（TMP）PK in healthy,chronically reserpinized rats（rat model ofspleen deficiency syndrome,RMSDS）andRMSDS treated with Sijunzi decoction（SJZD）forconfirmation were used to verify S-PK; secondly,the ferulic acid（FA）PK in healthy andhigh molecular weight dextran（HMWD）-inducedrabbit model with blood stasis syndrome（RDBSS）was also used to verify S-PK;andlastly,TMP PK parameters in serum of healthyrats after orally taken Ligusticum wallichii（LW）,LW and Salvia miltiorrhiza（LW&SM）decoctions were compared to verify R-PK.RESULTS The apparent first-order absorption[Ka,（13.61±2.56）h<sup>-1</sup>,area under the blooddrug concentration-time curve[AUC,（24.88±9.76）μg.h<sup>-1</sup>mL<sup>-1</sup>],maximum drug concentration[C<sub>max</sub>,（4.82±1.23）μg·mL<sup>-1</sup>]of serum TMP inRMSDS were increased markedly（P【0.05）compared with those[Ka=（5.41±1.91）h<sup>-1</sup>,AUC=（5.20±2.57）μg·h<sup>-1</sup>·mL<sup>-1</sup>,C<sub>max</sub>=（2.33±1.77）μg·mL<sup>-1</sup>]of healthy rats（HR）.Theapparent first-order rate constant for α and βdistribution phase[α=（0.38±0.09）h<sup>-1</sup>,β=（0.06±0.03）h<sup>-1</sup>,the apparent first-orderintercompartmental transfer rate constants[K<sub>10</sub>=（0.24±0.07）h<sup>-1</sup>,K<sub>12</sub>=（0.11±0.02）h<sup>-1</sup>,K<sub>21</sub>=（0.11±0.02）h<sup>-1</sup>]of serum TMP in RMSDS weredecreased significantly（P【0.01）comparedwith those[K<sub>10</sub>=（0.88±0.20）h<sup>-1</sup>,K<sub>12</sub>=（1.45±0.47）h<sup>-1</sup>,K<sub>21</sub>=（0.72±0.22）h<sup>-1</sup>]of HR.However,no apparent differences occurredbetween HR and RMSDS treated with SJZD.Theserum FA concentration and its AUC[（5.6690±2.3541）μg·h<sup>-1</sup>·mL<sup>-1</sup>] in RMBSS were also higherthan those[AUC=（2.7566±0.8232）μg·h<sup>-1</sup>·mL<sup>-1</sup>]of healthy rabbits（P【0.05）.The Ka（11.51±2.82）h<sup>-1</sup>,AUC（0.84±0.17）μg·h<sup>-1</sup>·mL<sup>-1</sup>of LW & SM-derived TMP in serum weremuch lower（P【0.05）than those[Ka=（19.58±4.14）h<sup>-1</sup>,AUC=（1.27±0.26）μg·h<sup>-1</sup>·mL<sup>-1</sup>]ofLW-derived TMP in serum after oral decoctions.CONCLUSION The SDS and blood stasissyndrome state could affect significantly thepharmacokinetic parameters of drugs and theabnormal SDS pharmacokinetic parameters couldbe normalized by SJZD.The combination ofChinese medicine in TCR could reciprocallyaffect the pharmacokinetic parameters of othercomponents absorbed into the systemiccirculation.These results support the S-and R-PK hypothesis.展开更多
Objective: MicroRNAs(miRNAs) may be viable targets for treating renal interstitial fibrosis(RIF). Fuzheng Huayu recipe(FZHY), a traditional Chinese compound herbal medicine, is often used in China to treat fibrosis. T...Objective: MicroRNAs(miRNAs) may be viable targets for treating renal interstitial fibrosis(RIF). Fuzheng Huayu recipe(FZHY), a traditional Chinese compound herbal medicine, is often used in China to treat fibrosis. This study sought to assess the mechanisms through which FZHY influences miRNAs to treat RIF.Methods: RIF was induced in rats by mercury chloride and treated with FZHY. Hydroxyproline content,Masson’s staining and type I collagen expression were used to evaluate renal collagen deposition.Renal miRNA profiles were evaluated using a miRNA microarray. Those miRNAs that were differentially expressed following FZHY treatment were identified and subjected to bioinformatic analyses. The miR-21 target gene phosphatase and tensin homolog(PTEN) expression and AKT phosphorylation in kidney tissues were assessed via Western blotting. In addition, HK-2 human proximal tubule epithelial cells were treated using angiotensin II(Ang-II) to induce epithelial-to-mesenchymal transition(EMT), followed by FZHY exposure. miR-21 and PTEN expressions were evaluated via quantitative reverse transcriptionpolymerase chain reaction(qRT-PCR), while E-cadherin and a-smooth muscle actin(a-SMA) expressions were assessed by immunofluorescent staining and qRT-PCR. Western blotting was used to assess PTEN and AKT phosphorylation.Results: FZHY significantly decreased kidney collagen deposition, hydroxyproline content and type I collagen level. The miRNA microarray identified 20 miRNAs that were differentially expressed in response to FZHY treatment. Subsequent bioinformatic analyses found that miR-21 was the key fibrosis-related miRNA regulated by FZHY. FZHY also decreased PTEN expression and AKT phosphorylation in fibrotic kidneys. Results from in vitro tests also suggested that FZHY promoted E-cadherin upregulation and inhibited a-SMA expression in Ang-II-treated HK-2 cells, effectively reversing Ang-II-mediated EMT. We also determined that FZHY reduced miR-21 expression, increased PTEN expression and decreased AKT phosphorylation in these cells.Conclusion: miR-21 is the key fibrosis-related miRNA regulated by FZHY. The ability of FZHY to modulate miR-21/PTEN/AKT signaling may be a viable approach for treating RIF.展开更多
OBJECTIVE:To observe the effects of Liandouqingmai Recipe on life quality and vascular endothelial injury in patients with coronary heart disease.METHODS:Capitalized 101 patients with coronary heart disease were rando...OBJECTIVE:To observe the effects of Liandouqingmai Recipe on life quality and vascular endothelial injury in patients with coronary heart disease.METHODS:Capitalized 101 patients with coronary heart disease were randomly divided into a treatment group(n=45) treated with Liandouqingmai Recipe and a standard treatment group(control group,n=56).A normal group of 16 healthy persons was additionally set up.Changes in ET-1 and NO levels were measured and Seatle Angina Questionnaire(SAQ) was adopted in studying life quality before and after treatment for two weeks.The data were analyzed with SPSS 16.0 statistic software.RESULTS:The average level of ET-1 in the normal group was lower and NO higher than that of patients with coronary heart disease.There was no significant difference in the average level of ET-1 and NO and in the scores of SAQ [physical limitation(PL),pngina stability(AS),apngina frequency(AF),treatment satisfaction(TS) and disease perception(DP)] between the two groups before treatment(P>0.05).But after treatment,the scores of SAQ(PL,AS,AF,TS,DP) and NO level were higher than those in the control group,and ET-1 average level in the treatment group was lower than that in the control group.The negative relations between PL and ET-1 and between AF and ET-1 were found in this study.CONCLUSION:Liandouqingmai Recipe can raise scores of SAQ and NO level and decline ET level in patients with coronary heart disease on the basis of convertional standard treatment,thus improving vascular endothelial function and life quality.Life quality is related to vascular endothelial function.展开更多
Objective:To observe effects of Liandou Qingmai Recipe(连豆清脉方) on endothelin-1(ET-1),nitric oxide(NO),interleukin-6(IL-6) and IL-10 levels in patients with coronary heart disease.Methods:Total 101 cases with coron...Objective:To observe effects of Liandou Qingmai Recipe(连豆清脉方) on endothelin-1(ET-1),nitric oxide(NO),interleukin-6(IL-6) and IL-10 levels in patients with coronary heart disease.Methods:Total 101 cases with coronary heart disease were randomly divided into a treatment group(n=45) treated by Liandou Qingmai Recipe and a standard treatment group(control group,n=56),with a normal group of 16 health persons set up.Changes of ET-1,NO,IL-6 and IL-10 levels were measured before treatment and after treatment for two weeks.And the data were analyzed by SPSS 16.0 statistic software.Results:Before treatment,the levels of ET-1,IL-6 and IL-10 levels were significantly higher and NO was significantly lower in the patients with coronary heart disease than those in the normal group(90.7±12.7 ng/L vs 41.8±13.5 ng/L,9.17±0.18 ng/L vs 1.10±0.08 ng/L,1.94±0.26 ng/L vs 1.09±0.06 ng/L,and 92.2±17.7 μmol/L vs 124.5±27.2 μmol/L;all P<0.05),with no significant differences in the levels of ET-1,NO,IL-6 and IL-10 between the treatment group and the control group(P>0.05);After treatment,ET-1 and IL-6 significantly decreased in the treatment group and the control group,and NO increased in the treatment group;And IL-6 level was significantly lower and NO level was higher in the treatment group than those in the control group(4.48±1.22 ng/L vs 5.13±1.85 ng/L,117.4±22.3 μmol/L vs 92.4±17.1 μmol/L;both P<0.05);There was a positive correlation between IL-6 and IL-10,and a negative correlation between NO and IL-10(r=0.142,r=-0.152;both P<0.05).Conclusion:Liandou Qingmai Recipe can decline IL-6,IL-10 and ET-1 levels,and raise NO level in patients with coronary heart disease on the basis of standard treatment,so as to inhibit endothelial inflammatory response,improve vascular endothelial function,with stronger anti-AS action;And vascular endothelial lesion is related with inflammatory response.展开更多
基金Beijing University of Chinese Medicine Transversal Project:"Internal and External"Product and Technology Development for Herbal Acne and Blemish Removal(No.2019110031001241)the Youth Project Under the National Natural Science Foundation of China:Revealing the Scientific Connotation of Tongfu Chinese Herb Rhubarb in Treating Ischemic Stroke from the Perspective of"Intestinal Tryptophan Metabolism and Central Microglia Polarisation"(No.82104440)。
文摘OBJECTIVE:To analyze the chemical components of Mudan Huaban recipe(牡丹化斑方,MHR)and evaluate its efficacy and possible mechanism in melasma mice.METHODS:The chemical compositions of MHR were determined by the ultra-high performance liquid chromatography coupled with quadrupole-exactive mass spectrometry method.Female C57BL/6 mice were exposed to ultraviolet B and progesterone for 21 d to induce melasma,and Fontana-Masson staining was used to assess the effects of MHR on melasma.Luteinizing hormone(LH),estradiol,and follicle-stimulating hormone levels were detected by enzyme-linked immunosorbent assay.The superoxide dismutase(SOD)activity and malonic dialdehyde content were detected by chemiluminescence.Tyrosinase and related proteins expressions were detected by Western blots and immunohistochemistry.RESULTS:A total of 43 chemical components were identified in MHR,MHR significantly decreased the melanin particles of melasma mice.MHR treatment significantly reversed the high contents of LH and low activity of SOD in models.MHR significantly reduced the higher levels of tyrosinase,tyrosinase-related proteins-1(TRP-1),TRP-2,microphthalmia-associated transcription factor and phosphorylation of cyclic adenosine monophosphate response element-binding protein(pCREB)/CREB in the skin of melasma mice.CONCLUSIONS:MHR protects against melasma via regulation of sex hormones,oxidative stress,and melanogenesis-related proteins,suggesting its possible use as a supplement and alternative drug therapy for melasma.
基金supported by National Chinese Medicine Inheritance and Development Demonstration Pilot Project-Zhongshan Chinese Medicine Hospital Chinese Medicine Research Project(YN2024B005).
文摘Objective To investigate the active components and mechanisms by which Qingre Zaoshi Jiedu Recipe influences the transformation from cervical high-risk human papillomavirus(HR-HPV)-related cervicitis to cancer,utilizing network pharmacology,Gene Expression Omnibus(GEO)data,and molecular docking techniques.Methods Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),HERB,and the Encyclopedia of Traditional Chinese Medicine(ETCM)databases were used to screen out the active ingredients and related targets of Qingre Zaoshi Jiedu Recipe.The GeneCards database for disease targets at different stages of HR-HPV-related cervicitis–cancer were searched.The String platform to construct a protein-protein interaction network and identify key targets was utilized.Enrichment analysis of intersecting genes was performed using the DAVID database.The GSE149763 dataset from the GEO to identify differential genes involved in the transformation from cervicitis to cervical cancer by comparing cervicitis with cervical intraepithelial neoplasia III and cervical cancer was utilized.R language to generate volcano plots,heat maps,and key target expression trend charts were employed.Molecular docking of key pathway targets and main compounds of Qingre Zaoshi Jiedu Recipe for HR-HPV-related cervicitis–cancer was performed using AutoDock Vina.Results The study identified 185 main active ingredients of Qingre Zaoshi Jiedu Recipe.The protein–protein interaction network indicates that the core targets for interfering with HR-HPV-related inflammation–cancer transformation include TNF,IL-6,IL-1β,CXCL8,IL-1α,IFN-γ,IL-10,CCL2,CCL5,and CXCL10.KEGG pathway analysis indicates that Qingre Zaoshi Jiedu Recipe primarily affects HR-HPV-related inflammation–cancer transformation via the Toll-like receptor signaling pathway.GEO analysis identified the Toll-like receptor pathway as crucial across various stages of cervicitis–cancer lesions,with CXCL10 emerging as a key target.Molecular docking analysis revealed that the primary components of Qingre Zaoshi Jiedu Recipe effectively bind to TLR4.Conclusion Qingre Zaoshi Jiedu Recipe can interfere with HR-HPV-related cervicitis–cancer transformation by acting on TLR4 through the Toll-like receptor pathway.
文摘OBJECTIVES:To investigate the effect of Bushen Tongluo recipe(BSTLR, 补肾通络方) on rats with diabetic kidney disease(DKD) and to explore the underlying mechanism of action. METHODS:The rat model of DKD was established, and rats were treated with different doses of BSTLR. Body weight and the levels of urinary protein, α1-microglobulin, glucose, blood urea nitrogen, creatinine, Cystatin C, superoxide dismutase, malondialdehyde, and catalase were analyzed biochemically or by enzyme-linked immunosorbent assay. The pathological damage to renal tissues was assessed by hematoxylin-eosin staining. Immunohistochemical staining was carried out to detect the expression levels of fibronectin, E-cadherin, α-smooth muscle actin, laminin, vimentin, collagen type Ⅳ in kidney tissues. Western blot analysis was conducted to analyze the expression levels of Nephrin, Desmin, Podocin, transforming growth factor-β1, mothers against decapentaplegic homolog 3(Smad3), Notch1, jagged, hairy and enhancer of split 1(Hes1) in kidney tissues, and the expression levels of maternally expressed gene 3(MEG3) and mi R-145 were measured by quantitative reverse transcription-polymerase chain reaction. Moreover, dual-luciferase reporter assay was employed to verify the binding of mi R-145 to MEG3. RESULTS:BSTLR increased the body weight of DKD rats, effectively ameliorated the renal function and pathological injury in DKD, regulated the balance of renal oxidative stress, inhibited the TGF/Notch signaling pathway, and affected the variations in the lnc RNA MEG3/mi R-145 axis. CONCLUSION:BSTLR improved oxidative stress homeostasis, inhibited the TGF/Notch signaling pathway, and regulated the lnc RNA MEG3/mi R-145 axis, effectively delaying the progression of DKD.
基金funded by the Program of Shanghai Municipal Health Commission (No.19401970400)the National Natural Science Foundation of China (No.82174130 and No.82274262)Shanghai Collaborative Innovation Center of Industrial Transformation of Hospital TCM Preparation。
文摘Background:Hypertension,a prevalent disease,is a significant risk factor for coronary heart disease.Huoxue Qianyang Qutan Recipe (HQQR),a traditional Chinese herbal remedy,has been used for treating hypertension over several years.Objective:This study assesses HQQR’s efficacy for controlling blood pressure among patients with hypertension related to blood stasis,yang hyperactivity and phlegm.Design,setting,participants and interventions:A randomized controlled trial was conducted at the Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,China,from July 2020 to June 2022.Major components of HQQR were identified using thin-layer chromatography and high-performance liquid chromatography.Participants aged18–80 years,exhibiting traditional Chinese medicine syndromes of blood stasis,yang hyperactivity or phlegm,along with grades 1 or 2 hypertension,were randomly categorized into two groups.The intervention group was given HQQR granules alongside conventional hypertension treatment,while the control group was given placebo granules in addition to conventional treatment for 12 weeks.Main outcome measures:The primary outcome was clinic blood pressure,whereas secondary outcomes included metabolic indices (e.g.,homeostasis model assessment of insulin resistance[HOMA-IR],total cholesterol[TC],low-density lipoprotein cholesterol and triglyceride),target organ damage indices (left ventricular mass index and urinary albumin creatinine ratio[UACR]) and inflammation indices(interleukin-6[IL-6]and high-sensitivity C-reactive protein[hs-CRP]).Results:HQQR’s primary components were identified as salvianolic acid B,emodin and ferulic acid.Of the 216 participants (108 in each group),compared to the control,the intervention group exhibited significant improvements (P<0.001) in clinic systolic blood pressure ([136.24±7.63]vs[130.06±8.50]mmHg),clinic diastolic blood pressure ([84.34±8.72]vs[80.46±6.05]mmHg),home systolic blood pressure([131.64±8.74]vs[122.36±8.45]mmHg) and home diastolic blood pressure ([78.47±9.53]vs[71.79±6.82]mm Hg).HQQR demonstrated a reduction in ambulatory blood pressure (24-hour systolic blood pressure:[133.75±10.49]vs[132.46±8.84]mmHg and 24-hour diastolic blood pressure:[84.12±8.01]vs[82.11±7.45]mmHg) and an improvement in HOMA-IR ([4.09±1.72]vs[3.98±1.44]),TC ([4.66±1.47]vs[3.75±1.81]mmol/L) and UACR (75.94[5.12,401.12]vs 45.61[4.26,234.26]).Moreover,HQQR demonstrated a decrease in hs-CRP (1.46[0.10,10.53]vs 0.57[0.12,3.99]mg/L) and IL-6 (6.69[2.00,29.74]vs 5.27[2.00,9.73]pg/mL),with no reported side effects (P<0.001).Conclusion:This study highlights the therapeutic potential of HQQR use in ameliorating blood pressure,glycolipid metabolism,and inflammation in patients with hypertension.
基金the National Natural Science Foundation of China:the Effect of Phosphatase and Tensin Homolog-induced Putative Kinase 1/Parkin Mediated Mitochondrial Autophagy on Myocardial Injury in Chronic Kidney Disease and the Intervention Mechanism of Shenshuai Recipe(No.81960843)Training Plan for Young and Middle-aged Key Talents Project of Traditional Chinese Medicine of Jiangxi Province(No.[2022]7)。
文摘OBJECTIVE:To study whether Shenshuai recipe(肾衰方,SSR)can play a protective role on chronic kidney disease myocardial injury model through phosphatase and tensin homolog-induced putative kinase 1(PINK1)/E3 ubiquitin ligase Parkin(Parkin)mitochondrial autophagy pathway.METHODS:Forty-eight nephrectomized rats were randomly divided into six groups:sham-operated group,model group,Benazepril group,low,medium and highdose groups of SSR.The rats were given the corresponding intervention for six weeks,then were sacrificed.Serum was examined by enzyme linked immunosorbent assay(ELISA).Cardiac ultrasound was used to detect cardiac function in 5/6 nephrectomized rats.Myocardial tissue was examined by light and electron microscopy;PINK1,Parkin,microtubule-associated protein 1 light chain 3 II(LC3B),sequestosome 1(P62),BECN1(Beclin-1)and dynamin-related protein 1(Drp-1)were measured by real time polymerase chain reaction(RT-PCR),Western blot(WB)and immunohistochemistry(IHC).RESULTS:The expression levels of blood urea nitrogen(BUN)and creatinine(SCr)in the model group were significantly higher than those in the sham-operated group,indicating that modeling was successful.SSR can protect myocardium by reducing the relative expression of creatine kinase myocardial isoenzyme and hypersensitivity cardiac troponin I(P<0.05).SSR can improve cardiac function in rats after ultrasound testing.SSR can improve the pathological manifestations of myocardial tissue after Masson staining.SSR can increase the number of autophagosomes and autophagiclysosomes in 5/6 nephrectomized rats(P<0.05).Determined by RT-PCR,WB and IHC,SSR can increase the relative expression of PINK1,Parkin,and LC3B(P<0.05),and decrease the relative expression of P62,Beclin-1 and Drp-1(P<0.05).CONCLUSIONS:The PINK1/Parkin mitochondrial autophagy pathway in myocardial tissues in 5/6 nephrectomy CKD myocardial injury rats was inhibited.SSR can activate PINK1/Parkin mitochondrial autophagy to enhance mitochondrial autophagy,and play a protective role in myocardial tissues.
基金Natural Science Foundation-funded Project:Effect of Macrophage-to-Myofibroblast Transition in Contralateral Kidney of Unilateral Ureteral Obstruction Rats Through the Aldosterone/MR/SGK1 Pathway and Inhibition of Chinese Herbs(No.81873251)Natural Science Foundation-funded Project:Aldosterone Stimulates MR Activation to Induce Lymphangiogenesis in the Contralateral Kidney of UUO Rats and the Protective Effect of Yiqi Jiedu Huayu Herbs(No.82174317)+1 种基金Hebei Provincial Postgraduate Innovative Ability Cultivation Funding Project:Aldosterone Induced Macrophages to Secrete VEGFA to Participate in Renal Angiogenesis and the Protective Effect of Huoxue Jiedu Huayu Recipe(CXZZBS2023140)Construction Program of New Research and Development Platform and Institution,Hebei Province Innovation Ability Promotion Plan under Grant No.20567624H。
文摘OBJECTIVE:To elucidate the mechanism by which Huoxue Jiedu Huayu recipe(活血解毒化瘀方,HJHR)regulates angiogenesis in the contralateral kidney of unilateral ureteral obstruction(UUO)rats and the mechanism by which it reduces of renal fibrosis.METHODS:Male Wistar rats were randomly divided into 4 groups:the sham group,UUO group(180 d of left ureter ligation),UUO plus eplerenone(EPL)group,and UUO plus HJHR group.After 180 d of oral drug administration,blood and contralateral kidneys were collected for analysis.Angiogenesis-and fibrosis-related indexes were detected.RESULTS:HJHR and EPL improved structural damage and renal interstitial fibrosis in the contralateral kidney and reduced the protein expression levels ofα-smooth muscle actin(α-SMA),vimentin and collagen I.Moreover,these treatments could reduce the expression of vascular endothelial growth factor-A(VEGFA)by inhibiting the infiltration of macrophages.Furthermore,HJHR and EPL significantly reduced the expression of CD34 and CD105 by downregulating VEGFA production,which inhibited angiogenesis.Finally,the coexpressions of CD34,CD105 andα-SMA were decreased in the HJHR and EPL groups,indicating that endothelial-to-mesenchymal transition was inhibited.CONCLUSIONS:These findings confirm that HJHR alleviates contralateral renal fibrosis by inhibiting VEGFAinduced angiogenesis,encourage the use of HJHR against renal interstitial fibrosis and provide a theoretical basis for the clinical management of patients with CKD.
基金Supported by 2021 Shenyang Science and Technology Program-Public Health R&D Special Project(Joint Project)of Shenyang Municipal Science and Technology Bureau,No.21-174-9-04.
文摘BACKGROUND Chronic heart failure is a complex clinical syndrome.The Chinese herbal compound preparation Jianpi Huatan Quyu recipe has been used to treat chronic heart failure;however,the underlying molecular mechanism is still not clear.AIM To identify the effective active ingredients of Jianpi Huatan Quyu recipe and explore its molecular mechanism in the treatment of chronic heart failure.METHODS The effective active ingredients of eight herbs composing Jianpi Huatan Quyu recipe were identified using the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform.The target genes of chronic heart failure were searched in the Genecards database.The target proteins of active ingredients were mapped to chronic heart failure target genes to obtain the common drugdisease targets,which were then used to construct a key chemical componenttarget network using Cytoscape 3.7.2 software.The protein-protein interaction network was constructed using the String database.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were performed through the Metascape database.Finally,our previously published relevant articles were searched to verify the results obtained via network pharmacology.RESULTS A total of 227 effective active ingredients for Jianpi Huatan Quyu recipe were identified,of which quercetin,kaempferol,7-methoxy-2-methyl isoflavone,formononetin,and isorhamnetin may be key active ingredients and involved in the therapeutic effects of TCM by acting on STAT3,MAPK3,AKT1,JUN,MAPK1,TP53,TNF,HSP90AA1,p65,MAPK8,MAPK14,IL6,EGFR,EDN1,FOS,and other proteins.The pathways identified by KEGG enrichment analysis include pathways in cancer,IL-17 signaling pathway,PI3K-Akt signaling pathway,HIF-1 signaling pathway,calcium signaling pathway,cAMP signaling pathway,NF-kappaB signaling pathway,AMPK signaling pathway,etc.Previous studies on Jianpi Huatan Quyu recipe suggested that this Chinese compound preparation can regulate the TNF-α,IL-6,MAPK,cAMP,and AMPK pathways to affect the mitochondrial structure of myocardial cells,oxidative stress,and energy metabolism,thus achieving the therapeutic effects on chronic heart failure.CONCLUSION The Chinese medicine compound preparation Jianpi Huatan Quyu recipe exerts therapeutic effects on chronic heart failure possibly by influencing the mitochondrial structure of cardiomyocytes,oxidative stress,energy metabolism,and other processes.Future studies are warranted to investigate the role of the IL-17 signaling pathway,PI3K-Akt signaling pathway,HIF-1 signaling pathway,and other pathways in mediating the therapeutic effects of Jianpi Huatan Quyu recipe on chronic heart failure.
基金supported by the grants from National Natural Science Foundation of China(No.82030117,82074203,82170033,and 82374540)Special Fund for Research on Community Medicine and Health Management in Shanghai(No.2023SQ01)+2 种基金Medical Research Project of Health Commission of Shanghai Hongkou District(No.HW2302-43)Special Medical Basic Research Project of the First Affiliated Hospital of Naval Medical University(No.2021JCMS12)Wild Goose Array Project of Zhengzhou Center of Chinese People’s Liberation Army Joint Logistic Support Force。
文摘Objective:Tumor-derived exosomes(TDEs)play crucial roles in intercellular communication.Hypoxia in the tumor microenvironment enhances secretion of TDEs and accelerates tumor metastasis.Jiedu recipe(JR),a traditional Chinese medicinal formula,has demonstrated efficacy in preventing the metastasis of hepatocellular carcinoma(HCC).However,the underlying mechanism remains largely unknown.Methods:Animal experiments were performed to investigate the metastasis-preventing effects of JR.Bioinformatics analysis and in vitro assays were conducted to explore the potential targets and active components of JR.TDEs were assessed using nanoparticle tracking analysis(NTA)and Western blotting(WB).Exosomes derived from normoxic or hypoxic HCC cells(H-TDEs)were collected to establish premetastatic mouse models.JR was intragastrically administered to evaluate its metastasis-preventive effects.WB and lysosomal staining were performed to investigate the effects of JR on lysosomal function and autophagy.Bioinformatics analysis,WB,NTA,and immunofluorescence staining were used to identify the active components and potential targets of JR.Results:JR effectively inhibited subcutaneous-tumor-promoted lung premetastatic niche development and tumor metastasis.It inhibited the release of exosomes from tumor cells under hypoxic condition.JR treatment promoted both lysosomal acidification and suppressed secretory autophagy,which were dysregulated in hypoxic tumor cells.Quercetin was identified as the active component in JR,and the epidermal growth factor receptor(EGFR)was identified as a potential target.Quercetin inhibited EGFR phosphorylation and promoted the nuclear translocation of transcription factor EB(TFEB).Hypoxia-impaired lysosomal function was restored,and secretory autophagy was alleviated by quercetin treatment.Conclusion:JR suppressed HCC metastasis by inhibiting hypoxia-stimulated exosome release,restoring lysosomal function,and suppressing secretory autophagy.Quercetin acted as a key component of JR and regulated TDE release through EGFR-TFEB signaling.Our study provides a potential strategy for retarding tumor metastasis by targeting H-TDE secretion.
基金a Special Clinical Research Initiative for the Health Business Sponsored by the Shanghai Municipal Health Commission:a Multicenter Randomized Controlled Clinical Study of Longteng Tongluo Recipe for the Treatment of Lung Cancer Pain(202040155)Xinglin Youth Talent Training System of Shanghai University of Traditional Chinese Medicine Xinglin Young Scholars:Study The Mechanism of Longteng Tongluo Recipe in Cancer Pain based on The Theory of Tonifying Yuan Qi and Detoxification(RC-2017-02-02)+2 种基金the Shanghai Municipal Science and Technology Commission's Special Biomedical Technology Support Plan:External Treatment of Cancer Pain of Traditional Chinese Medicine to Promote Percutaneous Absorption of Ultrasound Instrument(20S31904100)Science and Technology Commission of Shanghai Municipality:Shanghai Clinical Research Center of Traditional Chinese Medicine Oncology(21MC1930500)Science Foundation of Yueyang Hospital:Discussion on the Anti-apoptotic Effect of Qidongning Prescription on Lung Cancer Based on Energy Metabolismin Disorder of Mitochondria(2021yygq05)。
文摘OBJECTIVE:To investigate the efficacy of Longteng Tongluo recipe(龙藤通络方,LTTL)combined with threestep analgesia for the treatment of lung cancer pain,and the changes in serum miRNA expressions before-and after treatment with LTTL and its correlation with lung cancer pain.The possible mechanism underlying LTTL effects on the treatment of lung cancer pain was conducted.METHODS:The pilot study was conducted at the oncology ward of the Yueyang Hospital and the Longhua Hospital between March 2018 and October 2019.A prospective,single-blind,placebo controlled,randomized clinical trial of LTTL or placebo combined with three-step analgesia treatments were administered to 24 cancer pain patients diagnosed with lung cancer.Analgesic efficacy was investigated as the primary outcome.Equivalent morphine consumption and numerical rating scale(NRS)scores were used as the secondary outcome.In the present study,we utilized deep sequencing techniques to compare the differential miRNA expressions in serum samples obtained from two groups:the lung cancer pain treatment group(LTTL+three-step analgesia)and the control group(placebo+three-step analgesia).Next,we employed the target prediction database to investigate the target genes for differential miRNA expressions and Gene Ontology(GO)analysis along with Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis to examine the roles and the major biochemical and signaling pathways related to the differentially expressed target genes,respectively.RESULTS:LTTL treatment significantly reduces the NRS score(P=0.021)as compared to those before treatment,along with significant reductions in the total morphine equivalent consumption(P=0.007)and the average daily equivalent morphine consumption(P=0.003)as opposed to the control group.The expressions of 31 miRNAs differed considerably between the two groups of patients(≥2 times up-modulated or down-regulated between these groups,P<0.05).For instance,the miRNAs expression levels for patients before treatment(has-miR-2110 and has-miR-7d-3p)were significantly enhanced as compared to the healthy people,after LTTL treatment,the expressions of miR-2110 and miR-7d-3p in patients with lung cancer pain reduced significantly.Studies show that the above two miRNAs were significantly associated with lung cancer pain,which could mediate lung cancer pain.Furthermore,we identified 355 genes as potential targets of the 31 differentially expressed miRNAs.Pathway enrichment analyses using KEGG and GO analysis indicated that these target genes may play a crucial role in the development and modulation of lung cancer pain.CONCLUSION:LTTL demonstrated a discernible impact on alleviating lung cancer pain and its mechanism of action may be related to the downregulation of has-miR-2110 and has-miR-7d-3p expressions.This pilot study provides support for further exploration of LTTL in patients with lung cancer pain.
基金the project of Traditional Chinese Medicine Bureau of Guangdong Province:Research on the Mechanism of Shen Shu Guan Xin Fang in Reducing Hypoxia Induced Myocardial Cell Injury by Regulating miR-24 (No. 20221108)Science and Technology Projects in Guangzhou:Exploring the Intervention of Shenzhu Guanxin Formula on Myocardial Ischemia Based on MiR-24 Mediated Bim/Caspase Apoptosis Signal Pathway Mechanism Research (No. 202201010521)+1 种基金the Science and Technology Innovation Projects of Shenzhen:to Study the Mechanism of Hesperidin in Improving Heart Failure Based on Myocardial Inflammation Mediated by MtDNAs GAS-STING Signaling Pathway (No. JCYJ20220530144212026)Futian Healthcare Research Project:To Study the Anti-atherosclerosis Effects and Mechanism of Shenzhu Guanxin Decoction on Inhibiting Platelet-mediated Inflammation Based on the Theory of "Phlegm-stasis Interjunction"(No. FTWS2022012)。
文摘OBJECTIVE: To evaluate the clinical efficacy and safety of Shenzhu Guanxin recipe granules(参术冠心颗粒, SGR) in treating patients with intermediate coronary lesions(ICL), and to investigate the potential mechanism though a transcriptome sequencing approach. METHODS: ICL patients with Qi deficiency and phlegm stasis were adopted and randomly assigned to a case group or a control by random number generator in a 1∶1 randomization ratio to evaluate the clinical efficacy. RESULTS: There was no significant difference between the two groups in coronary computed tomography angiography related indexes in the two groups before and after intervention. Through the gene chip expression analysis, it is finally concluded that there are 355 differential mRNAs(190 up-regulated genes and 165 down regulated genes) when compared the SGR group and placebo group. Through protein-protein interaction network analysis of differentially expressed genes, 10 hub genes were finally obtained: CACNA2D2, CACNA2D3, DNAJC6, FGF12, SGSM2, CACNA1G, LRP6, KIF25, OXTR, UPB1. CONCLUSIONS: SGR combined with Western Medicine can be safely used to treat ICL patients with Qi deficiency and phlegm stasis. The possible mechanism of action and relevant gene loci and pathway were proposed.
文摘Background:This study aims to explore the therapeutic effects of the Qi Teng Mai Ning recipe on ischemic stroke and vascular cognitive impairment through its potential to modulate cellular autophagy,with a focus on identifying its active ingredients and their target proteins.Methods:The study began with the identification of active ingredients in the Qi Teng Mai Ning recipe.It proceeded to screen the gene expression omnibus database for ischemic stroke and vascular cognitive impairment-associated differentially expressed mRNAs and to identify cellular autophagy-related proteins via the Human Autophagy Database.These proteins were annotated with Gene Ontology and Kyoto Encyclopedia of Genes and Genomes functions and subjected to molecular docking with the recipe’s core active ingredients.In vitro cell experiments were conducted on hypoxic HT22 cells,involving CCK8 assay,lentiviral transfection to silence autophagy related 9B(ATG9B),immunofluorescence staining,and qPCR validation to investigate the effects of the recipe on autophagy.Results:The analysis identified 104 active ingredients targeting 408 proteins and forming a complex ingredient-target network.Intersecting 55 ischemic stroke-related and 909 vascular cognitive impairment-related differentially expressed mRNAs revealed 14 co-expressed mRNAs.Molecular docking showed quercetin,kaempferol,myrcene,and conferone as key ingredients targeting autophagy-related proteins.Cellular experiments indicated that the recipe significantly enhanced cell viability under hypoxic conditions,reduced apoptosis,and modulated the expression of autophagy-related factors,thereby decreasing apoptosis rates in HT22 cells.Conclusion:The Qi Teng Mai Ning recipe offers protective effects against ischemic stroke and vascular cognitive impairment by modulating autophagy-related proteins.Its efficacy highlights the potential of traditional Chinese medicine in treating these conditions,though further research is needed to fully understand its mechanisms and clinical applications.
基金National Natural Science Foundation of China(No.82360934)Science and Technology Innovation Leading Talents Project of Xinjiang Uygur Autonomous Region(No.2022TSYCLJ0007)+1 种基金Xinjiang Uygur Autonomous Region Key Research and Development Task Special Project(No.2021B03006)Natural Science Foundat ion of Xinj iang Uygur Autonomous Region(No.2022D01C170,2022D01C171)。
文摘Objective:To observe the effect and possible mechanism of action of Bushen Bitong recipe(BSBT)containing serum on IL-1β-induced chondrocyte apoptosis.Methods:Generation 3 rat chondrocytes were randomized into Control,IL-1β,IL-1β+BSBT(L),IL-1β+BSBT(M),and IL-1β+BSBT(H)groups(5%,10%and 15%BSBT-containing serum),and then 24h after intervention respectively,the cell proliferation and Apoptosis rate;Western blot detected the expression levels of Bcl-2,BAX,Caspase-3,SOX9,NF-κB p65,MMP-13 proteins in chondrocytes.ELISA detected the levels of TNF-α,IL-6,and bFGF in the supernatants of chondrocyte culture.Results:Compared with Control group,cell proliferation activity decreased,apoptosis rate increased,NF-κB p65,MMP-13 protein level and TNF-α,IL-6 level increased,and SOX9 protein level and bFGF level decreased in IL-1βgroup;compared with IL-1βgroup,different concentrations of BSBT-containing serum group,cell proliferation activity increased,and apoptosis rate decreased.NF-κB p65,MMP-13 protein level and TNF-α,IL-6 level decreased,SOX9 protein level and bFGF level increased;compared with IL-1β+BSBT(L)group,cell proliferation activity increased,apoptosis rate decreased in IL-1β+BSBT(M)and IL-1β+BSBT(H)groups,and NF-κB p65,MMP-13 protein level and TNF-αlevel decreased.13 protein levels and TNF-αand IL-6 levels decreased,and SOX9 protein levels and bFGF levels increased.Conclusion:BSBT-containing serum may promote IL-1β-induced proliferation of chondrocytes,reduce apoptosis,improve the microenvironment of chondrocytes,and promote cartilage repair through the SOX9/NF-κB/MMP-13 signaling pathway.
文摘Chronic viral hepatitis B (HBV) remains a major public health problem in Burkina Faso. Since access to diagnostic tests and treatments is limited because of their high cost, the majority of the population turn to traditional herbal treatments. This study aimed to evaluate the effectiveness of a plant recipe called Hepatib tiben. It consisted of comparing certain biochemical and molecular parameters of patients infected with HBV that were supported by the recipe. The patients were recruited in Ouagadougou by the traditional health practitioner according to the requirements of the study. Thus 44 patients aged 20 to 61 years and carrier of HBsAg for at least 06 months were treated with Hepatib tiben. The tests were performed in the laboratory before and three months after the treatment. ELISA tests were used to confirm the presence of HBsAg and search for anti-HCV antibodies;transaminases, creatinine were quantified by the “Chem 400” automaton and the viral load of HBV by Real-time PCR. The analysis of the results reveals an improvement of the biochemical and molecular parameters of the patients with the following means (ASAT: 21.02 ± 9.97;ALAT: 21.11 ± 13.27;DNA: 1571.82 ± 3990.97 with p = 0.01 for each). As for HBsAg, its disappearance was observed in 4.55% of patients after treatment. The evaluation of the creatinine parameter explained that the recipe of plants has a tolerated effect on the kidneys of treated patients. These results, while encouraging, need to be complemented by further research for the development of effective phytomedicine to treat and eliminate this viral hepatitis B virus.
文摘[Objectives]To observe the therapeutic effect of intragastric administration of Jiangtang Shuxin recipe on diabetic heart failure(DHF)in rats and to explore its mechanism.[Methods]Fifty SD rats were randomly divided into five groups,with 10 rats in each group.DHF models were prepared in the low-dose group,high-dose group,Western medicine group,and model group except the control group.Rats in the low-dose and high-dose groups were given 1.0 and 1.5 g/(kg·d)Jiangtang Shuxin recipe suspension by gavage,respectively.Rats in the Western medicine group were given gliquidone and benazepril by gavage for 2 months,and were fed with high-fat diet.Rats in the control group were fed with ordinary diet.Fasting blood glucose(FBG),serum triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),C-reactive protein(CRP),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),creatine kinase isoenzyme(CK-MB),lactate dehydrogenase(LDH),pathological morphology of myocardial tissue,NF-κB p65 protein and IκBαprotein were compared among groups.[Results]Compared with the control group,the levels of FBG,serum TG,LDL-C,CRP,IL-6,TNF-α,CK-MB and LDH increased,while the level of serum HDL-C decreased.The myocardial tissue was seriously damaged,and the expression of NF-κB p65 protein increased,while the expression of IκBαprotein decreased in the other four gruops(all P<0.05).Compared with the model group,the levels of FBG,serum TG,LDL-C,CRP,IL-6,TNF-α,CK-MB and LDH decreased,while the serum HDL-C level increased.The myocardial tissue damage was alleviated,and the expression of NF-κB p65 protein decreased,while the expression of IκBαprotein increased in the low-dose group,high-dose group and Western medicine group(all P<0.05).Compared with the Western medicine group,the levels of FBG,serum TG,LDL-C,CRP,IL-6,TNF-α,CK-MB and LDH decreased,and the level of serum HDL-C increased in the high-dose group(all P<0.05).[Conclusions]Jiangtang Shuxin recipe has a therapeutic effect on DHF in rats,with the best effect in the high-dose group.It can not only alleviate high glucose and high fat state,but also reduce myocardial injury and inflammation,and improve the pathological morphology of myocardial cells.The mechanism may be related to its inhibition of NF-кB signaling pathway.
文摘The consumption of a mixture of leafy vegetables has always been a practice anchored in the dietary habits of the people of Brazzaville. The objective of this study was to evaluate the acute and subacute toxicities of the aqueous extract of the recipe based on three leafy vegetables (Amaranthus hybridus L., Spinacia oleracea, Brassica campestris L.) in laboratory rodents. The aqueous extract of the food recipe was administered orally using a cannula. Regarding acute toxicity, the administration of single doses of 5000 and 10,000 mg/kg to mice had no significant effects on behavior. On the other hand, weight loss was significantly (**p < 0.01) compared to control mice (1 mL/100g). The results on subacute toxicity showed that daily administration of the aqueous extract of the food recipe at a dose of 1000 mg/kg for 21 days in rats resulted in a non-significant weight gain, and caused changes in some biochemical parameters including HDL-cholesterol levels, blood sugar levels and ALAT activity.
文摘BACKGROUND Traditional Chinese medicine(TCM)is widely used as an important complementary and alternative healthcare system for cancer treatment in Asian countries.Network pharmacology,which utilizes various database platforms and computer software to study the interactions between complex drug components in vivo,is particularly useful for studying the pharmacodynamic mechanisms of multi-pathway and multi-target Chinese medicines.AIM To explore the potential targets and function of Jianpi Yiwei Recipe treatment of gastric cancer(GC)through network pharmacology and molecular docking.METHODS Data on the components of Jianpi Yiwei Recipe(Radix Astragali,Radix Codonopsis,Agrimonia eupatoria,Atractylodes macrocephala Koidz.,Poria cocos,stirbaked rhizoma dioscoreae,Amomum villosum Lour.,fried Fructus Aurantii,pericarpium citri reticulatae,Rhizoma Pinelliae Preparata,and Radix Glycyrrhizae Preparata)were collected and screened by using the TCM systems pharmacology database and analysis platform(TCMSP).Then the targets of these compounds were predicted.GC-related targets were screened using the GeneCards database.Venn diagram was used to identify common targets.An active ingredient-core target interaction network and a protein-protein interaction(PPI)network were built.Moreover,we performed gene ontology(GO)functional annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses on the core targets and validated them by molecular docking.RESULTS TCMSP screening revealed 11 active components and 184 targets,whereas GeneCards found 10118 disease-related targets,with 180 shared targets between them.Topology analysis of the PPI network identified 38 targets,including ATK1,TP53,and tumor necrosis factor,as key targets for the treatment of GC by Jianpi Yiwei Recipe.Quercetin,naringenin,luteolin,etc.,may be the main active components of Jianpi Yiwei Recipe.GO enrichment analysis identified 2809,1218,and 553 functions related to biological process,molecular function,and cellular component,respectively.KEGG pathway enrichment analysis revealed 167 related pathways,mainly involved in cancer,endocrine resistance,and AGE-RAGE signaling in diabetic complication.Validation with molecular docking analysis showed docking of key active components with core targets.CONCLUSION Jianpi Yiwei Recipe plays a therapeutic role in GC through multiple components,targets,and pathways.These findings form a basis for follow-up exploration of Jianpi Yiwei Recipe in the treatment of GC.
基金National Natural Science Foundation of China,No.398709323967086539570870 and 39100139.
文摘AIM To propose a hypothesis defining theabsorption,distribution,metabolism andelimination of traditional Chinese recipe（TCR）-component in blood of healthy subjects andpatients,and estimate its correctness.METHODS The pharmacokinetics（PK）of samedose of drug was studied in the animal model oftraditional Chinese syndrome（S）and healthyanimals.The classification,terminology,concept and significance of the hypothesis wereset forth with evidence provided in the presentstudy.The hypotheses consisted of traditionalChinese syndrome PK（S-PK）and traditionalChinese recipe PK（R-PK）.Firstly,the observedtetramethylpyrazine（TMP）PK in healthy,chronically reserpinized rats（rat model ofspleen deficiency syndrome,RMSDS）andRMSDS treated with Sijunzi decoction（SJZD）forconfirmation were used to verify S-PK; secondly,the ferulic acid（FA）PK in healthy andhigh molecular weight dextran（HMWD）-inducedrabbit model with blood stasis syndrome（RDBSS）was also used to verify S-PK;andlastly,TMP PK parameters in serum of healthyrats after orally taken Ligusticum wallichii（LW）,LW and Salvia miltiorrhiza（LW&SM）decoctions were compared to verify R-PK.RESULTS The apparent first-order absorption[Ka,（13.61±2.56）h<sup>-1</sup>,area under the blooddrug concentration-time curve[AUC,（24.88±9.76）μg.h<sup>-1</sup>mL<sup>-1</sup>],maximum drug concentration[C<sub>max</sub>,（4.82±1.23）μg·mL<sup>-1</sup>]of serum TMP inRMSDS were increased markedly（P【0.05）compared with those[Ka=（5.41±1.91）h<sup>-1</sup>,AUC=（5.20±2.57）μg·h<sup>-1</sup>·mL<sup>-1</sup>,C<sub>max</sub>=（2.33±1.77）μg·mL<sup>-1</sup>]of healthy rats（HR）.Theapparent first-order rate constant for α and βdistribution phase[α=（0.38±0.09）h<sup>-1</sup>,β=（0.06±0.03）h<sup>-1</sup>,the apparent first-orderintercompartmental transfer rate constants[K<sub>10</sub>=（0.24±0.07）h<sup>-1</sup>,K<sub>12</sub>=（0.11±0.02）h<sup>-1</sup>,K<sub>21</sub>=（0.11±0.02）h<sup>-1</sup>]of serum TMP in RMSDS weredecreased significantly（P【0.01）comparedwith those[K<sub>10</sub>=（0.88±0.20）h<sup>-1</sup>,K<sub>12</sub>=（1.45±0.47）h<sup>-1</sup>,K<sub>21</sub>=（0.72±0.22）h<sup>-1</sup>]of HR.However,no apparent differences occurredbetween HR and RMSDS treated with SJZD.Theserum FA concentration and its AUC[（5.6690±2.3541）μg·h<sup>-1</sup>·mL<sup>-1</sup>] in RMBSS were also higherthan those[AUC=（2.7566±0.8232）μg·h<sup>-1</sup>·mL<sup>-1</sup>]of healthy rabbits（P【0.05）.The Ka（11.51±2.82）h<sup>-1</sup>,AUC（0.84±0.17）μg·h<sup>-1</sup>·mL<sup>-1</sup>of LW & SM-derived TMP in serum weremuch lower（P【0.05）than those[Ka=（19.58±4.14）h<sup>-1</sup>,AUC=（1.27±0.26）μg·h<sup>-1</sup>·mL<sup>-1</sup>]ofLW-derived TMP in serum after oral decoctions.CONCLUSION The SDS and blood stasissyndrome state could affect significantly thepharmacokinetic parameters of drugs and theabnormal SDS pharmacokinetic parameters couldbe normalized by SJZD.The combination ofChinese medicine in TCR could reciprocallyaffect the pharmacokinetic parameters of othercomponents absorbed into the systemiccirculation.These results support the S-and R-PK hypothesis.
基金supported by the National Natural Science Foundation of China (No. 81573810,81270053,81530101)China Postdoctoral Science Foundation (No. 2015T80445)National Science&Technology Major Project “Key New Drug Creation and Manufacturing Program” of China (No. 2019ZX09201001)。
文摘Objective: MicroRNAs(miRNAs) may be viable targets for treating renal interstitial fibrosis(RIF). Fuzheng Huayu recipe(FZHY), a traditional Chinese compound herbal medicine, is often used in China to treat fibrosis. This study sought to assess the mechanisms through which FZHY influences miRNAs to treat RIF.Methods: RIF was induced in rats by mercury chloride and treated with FZHY. Hydroxyproline content,Masson’s staining and type I collagen expression were used to evaluate renal collagen deposition.Renal miRNA profiles were evaluated using a miRNA microarray. Those miRNAs that were differentially expressed following FZHY treatment were identified and subjected to bioinformatic analyses. The miR-21 target gene phosphatase and tensin homolog(PTEN) expression and AKT phosphorylation in kidney tissues were assessed via Western blotting. In addition, HK-2 human proximal tubule epithelial cells were treated using angiotensin II(Ang-II) to induce epithelial-to-mesenchymal transition(EMT), followed by FZHY exposure. miR-21 and PTEN expressions were evaluated via quantitative reverse transcriptionpolymerase chain reaction(qRT-PCR), while E-cadherin and a-smooth muscle actin(a-SMA) expressions were assessed by immunofluorescent staining and qRT-PCR. Western blotting was used to assess PTEN and AKT phosphorylation.Results: FZHY significantly decreased kidney collagen deposition, hydroxyproline content and type I collagen level. The miRNA microarray identified 20 miRNAs that were differentially expressed in response to FZHY treatment. Subsequent bioinformatic analyses found that miR-21 was the key fibrosis-related miRNA regulated by FZHY. FZHY also decreased PTEN expression and AKT phosphorylation in fibrotic kidneys. Results from in vitro tests also suggested that FZHY promoted E-cadherin upregulation and inhibited a-SMA expression in Ang-II-treated HK-2 cells, effectively reversing Ang-II-mediated EMT. We also determined that FZHY reduced miR-21 expression, increased PTEN expression and decreased AKT phosphorylation in these cells.Conclusion: miR-21 is the key fibrosis-related miRNA regulated by FZHY. The ability of FZHY to modulate miR-21/PTEN/AKT signaling may be a viable approach for treating RIF.
基金supported by Bureau of Traditional Chinese Medicine of Jiangsu Province(No.HZ07097)
文摘OBJECTIVE:To observe the effects of Liandouqingmai Recipe on life quality and vascular endothelial injury in patients with coronary heart disease.METHODS:Capitalized 101 patients with coronary heart disease were randomly divided into a treatment group(n=45) treated with Liandouqingmai Recipe and a standard treatment group(control group,n=56).A normal group of 16 healthy persons was additionally set up.Changes in ET-1 and NO levels were measured and Seatle Angina Questionnaire(SAQ) was adopted in studying life quality before and after treatment for two weeks.The data were analyzed with SPSS 16.0 statistic software.RESULTS:The average level of ET-1 in the normal group was lower and NO higher than that of patients with coronary heart disease.There was no significant difference in the average level of ET-1 and NO and in the scores of SAQ [physical limitation(PL),pngina stability(AS),apngina frequency(AF),treatment satisfaction(TS) and disease perception(DP)] between the two groups before treatment(P>0.05).But after treatment,the scores of SAQ(PL,AS,AF,TS,DP) and NO level were higher than those in the control group,and ET-1 average level in the treatment group was lower than that in the control group.The negative relations between PL and ET-1 and between AF and ET-1 were found in this study.CONCLUSION:Liandouqingmai Recipe can raise scores of SAQ and NO level and decline ET level in patients with coronary heart disease on the basis of convertional standard treatment,thus improving vascular endothelial function and life quality.Life quality is related to vascular endothelial function.
基金supported by Bureau of Traditional Chinese Medicine of Jiangsu Province (No. HZ07097)
文摘Objective:To observe effects of Liandou Qingmai Recipe(连豆清脉方) on endothelin-1(ET-1),nitric oxide(NO),interleukin-6(IL-6) and IL-10 levels in patients with coronary heart disease.Methods:Total 101 cases with coronary heart disease were randomly divided into a treatment group(n=45) treated by Liandou Qingmai Recipe and a standard treatment group(control group,n=56),with a normal group of 16 health persons set up.Changes of ET-1,NO,IL-6 and IL-10 levels were measured before treatment and after treatment for two weeks.And the data were analyzed by SPSS 16.0 statistic software.Results:Before treatment,the levels of ET-1,IL-6 and IL-10 levels were significantly higher and NO was significantly lower in the patients with coronary heart disease than those in the normal group(90.7±12.7 ng/L vs 41.8±13.5 ng/L,9.17±0.18 ng/L vs 1.10±0.08 ng/L,1.94±0.26 ng/L vs 1.09±0.06 ng/L,and 92.2±17.7 μmol/L vs 124.5±27.2 μmol/L;all P<0.05),with no significant differences in the levels of ET-1,NO,IL-6 and IL-10 between the treatment group and the control group(P>0.05);After treatment,ET-1 and IL-6 significantly decreased in the treatment group and the control group,and NO increased in the treatment group;And IL-6 level was significantly lower and NO level was higher in the treatment group than those in the control group(4.48±1.22 ng/L vs 5.13±1.85 ng/L,117.4±22.3 μmol/L vs 92.4±17.1 μmol/L;both P<0.05);There was a positive correlation between IL-6 and IL-10,and a negative correlation between NO and IL-10(r=0.142,r=-0.152;both P<0.05).Conclusion:Liandou Qingmai Recipe can decline IL-6,IL-10 and ET-1 levels,and raise NO level in patients with coronary heart disease on the basis of standard treatment,so as to inhibit endothelial inflammatory response,improve vascular endothelial function,with stronger anti-AS action;And vascular endothelial lesion is related with inflammatory response.
