Background: The persistence of the rapid spread of the COVID-19 pandemic is linked to the appearance of several variants of SARS-CoV2 with an impact on biological diagnosis, treatment and vaccination. The United State...Background: The persistence of the rapid spread of the COVID-19 pandemic is linked to the appearance of several variants of SARS-CoV2 with an impact on biological diagnosis, treatment and vaccination. The United States Food and Drug Administration (FDA) has granted several SARS-CoV-2 detection tests Emergency Use Authorization (EUA) for diagnosis and better epidemiological surveillance. Thus, multiple RT-PCR tests have been developed and brought to market in order to meet the urgent need for the diagnosis of COVID-19. However, comparative data between these tests in clinical laboratories are scarcely available to assess their performance. Objective: To compare two molecular methods for detecting SARS-CoV-2: the RT-PCR, Allplex™2019-nCoV tests on CFX96 Bio-Rad and the Abbott m2000sp/rt RealTime SARS-CoV-2. Materials and Methods: Nasopharyngeal and oropharyngeal swabs were taken from patients to diagnose SARS-CoV-2 infection. For each sample, we searched for the virus with two different RT-PCR tests: 1) first on Abbott m2000 SARS-CoV-2 targeting the N and RdRp genes, 2) then on Allplex™2019-nCoV Assay looking for the E, N and RdRp genes. Results: Percentages of the agreement were calculated. A total of 100 samples that tested negative and 90 positives on Abbott m2000 SARS-CoV-2 were retested on Allplex™2019-nCoV. Overall agreement was 74.74% on all samples. The specific agreement was 84% and 64.4% respectively for negative and positive samples with the RealTime SARS-CoV-2 test. A positive correlation (r<sup>2</sup> = 0.63;p Conclusion: Our results showed good overall agreement between RT-PCR, Allplex™2019-nCoV and Abbott RealTime SARS-CoV-2 tests in the diagnosis of COVID-19. As the concordance is low for small viremias, the RT-PCR Allplex™2019-nCoV Assay would be better indicated during the acute and symptomatic phase of the disease.展开更多
Porcine Contagious Pleuropneumonia(PCP)is a respiratory infectious disease of pigs caused by Actinobacillus pleuropneumoniae.The disease has been prevalent in pig farms since it was first identified in 1957(Pattison e...Porcine Contagious Pleuropneumonia(PCP)is a respiratory infectious disease of pigs caused by Actinobacillus pleuropneumoniae.The disease has been prevalent in pig farms since it was first identified in 1957(Pattison et al.1957).展开更多
The accurate selection of operational parameters is critical for ensuring the safety,efficiency,and automation of Tunnel Boring Machine(TBM)operations.This study proposes a similarity-based framework integrating model...The accurate selection of operational parameters is critical for ensuring the safety,efficiency,and automation of Tunnel Boring Machine(TBM)operations.This study proposes a similarity-based framework integrating model-based boring indexes(derived from rock fragmentation mechanisms)and Euclidean distance analysis to achieve real-time recommendations of TBM operational parameters.Key performance indicators-thrust(F),torque(T),and penetration(p)-were used to calculate three model-based boring indexes(a,b,k),which quantify dynamic rock fragmentation behavior.A dataset of 359 candidate samples,reflecting diverse geological conditions from the Yin-Chao water conveyance project in Inner Mongolia,China,was utilized to validate the framework.The system dynamically recommends parameters by matching real-time data with historical cases through standardized Euclidean distance,achieving high accuracy.Specifically,the mean absolute error(MAE)for rotation speed(n)was 0.10 r/min,corresponding to a mean absolute percentage error(MAPE)of 1.09%.For advance rate(v),the MAE was 3.4 mm/min,with a MAPE of 4.50%.The predicted thrust(F)and torque(T)values exhibited strong agreement with field measurements,with MAEs of 270 kN and 178 kN∙m,respectively.Field applications demonstrated a 30%reduction in parameter adjustment time compared to empirical methods.This work provides a robust solution for real-time TBM control,advancing intelligent tunneling in complex geological environments.展开更多
Based on rain gauge data during 2008-2021 from national meteorological observation stations,this study investigated the performance of the precipitation field from the 1-km-resolution version of the China Atmospheric ...Based on rain gauge data during 2008-2021 from national meteorological observation stations,this study investigated the performance of the precipitation field from the 1-km-resolution version of the China Atmospheric Realtime Analysis(CARAS)over Hubei from the perspective of climatology,multiple-time scale variations,as well as fusion accuracy and detection capability at multiple temporal scales.The results show that CARAS precipitation can reproduce the spatial distribution patterns of climatological seasonal precipitation and rainy days well over the whole of Hubei compared with observational(OBS)precipitation,albeit deviations exist between CARAS and OBS in terms of magnitude.Moreover,high correlation and consistency between CARAS and OBS can be found in multiple-time scale variations over Hubei,with correlation coefficients of interannual,seasonal,and diurnal variation generally exceeding 0.85,0.98,and 0.95,respectively.Furthermore,CARAS has a relatively higher fusion accuracy in summer and winter,and stronger/weaker detection capability in spring/winter at a daily scale.However,the detection capability of CARAS at an hourly scale is weaker than that at a daily scale.With different precipitation intensity levels considered,CARAS daily precipitation shows relatively higher fusion accuracy in estimating moderate and heavy rain,and better detection capability in capturing no rain events.The variations of accuracy metrics and detection metrics under different precipitation intensities at an hourly scale generally resemble those at a daily scale.However,CARAS precipitation at an hourly scale shows a relatively lower fusion accuracy and weaker detection capability compared with that at a daily scale.This paper provides an insight into the characteristics of systematic deviations in CARAS precipitation over Hubei,which will benefit relevant applications of CARAS in meteorological operations over Hubei and the improvement of CARAS in the future.展开更多
文摘Background: The persistence of the rapid spread of the COVID-19 pandemic is linked to the appearance of several variants of SARS-CoV2 with an impact on biological diagnosis, treatment and vaccination. The United States Food and Drug Administration (FDA) has granted several SARS-CoV-2 detection tests Emergency Use Authorization (EUA) for diagnosis and better epidemiological surveillance. Thus, multiple RT-PCR tests have been developed and brought to market in order to meet the urgent need for the diagnosis of COVID-19. However, comparative data between these tests in clinical laboratories are scarcely available to assess their performance. Objective: To compare two molecular methods for detecting SARS-CoV-2: the RT-PCR, Allplex™2019-nCoV tests on CFX96 Bio-Rad and the Abbott m2000sp/rt RealTime SARS-CoV-2. Materials and Methods: Nasopharyngeal and oropharyngeal swabs were taken from patients to diagnose SARS-CoV-2 infection. For each sample, we searched for the virus with two different RT-PCR tests: 1) first on Abbott m2000 SARS-CoV-2 targeting the N and RdRp genes, 2) then on Allplex™2019-nCoV Assay looking for the E, N and RdRp genes. Results: Percentages of the agreement were calculated. A total of 100 samples that tested negative and 90 positives on Abbott m2000 SARS-CoV-2 were retested on Allplex™2019-nCoV. Overall agreement was 74.74% on all samples. The specific agreement was 84% and 64.4% respectively for negative and positive samples with the RealTime SARS-CoV-2 test. A positive correlation (r<sup>2</sup> = 0.63;p Conclusion: Our results showed good overall agreement between RT-PCR, Allplex™2019-nCoV and Abbott RealTime SARS-CoV-2 tests in the diagnosis of COVID-19. As the concordance is low for small viremias, the RT-PCR Allplex™2019-nCoV Assay would be better indicated during the acute and symptomatic phase of the disease.
基金supported by the University-Industry Col aborative Education Program,China(220904860093831)。
文摘Porcine Contagious Pleuropneumonia(PCP)is a respiratory infectious disease of pigs caused by Actinobacillus pleuropneumoniae.The disease has been prevalent in pig farms since it was first identified in 1957(Pattison et al.1957).
基金supported by the National Key R&D Program of China(2022YFE0200400).
文摘The accurate selection of operational parameters is critical for ensuring the safety,efficiency,and automation of Tunnel Boring Machine(TBM)operations.This study proposes a similarity-based framework integrating model-based boring indexes(derived from rock fragmentation mechanisms)and Euclidean distance analysis to achieve real-time recommendations of TBM operational parameters.Key performance indicators-thrust(F),torque(T),and penetration(p)-were used to calculate three model-based boring indexes(a,b,k),which quantify dynamic rock fragmentation behavior.A dataset of 359 candidate samples,reflecting diverse geological conditions from the Yin-Chao water conveyance project in Inner Mongolia,China,was utilized to validate the framework.The system dynamically recommends parameters by matching real-time data with historical cases through standardized Euclidean distance,achieving high accuracy.Specifically,the mean absolute error(MAE)for rotation speed(n)was 0.10 r/min,corresponding to a mean absolute percentage error(MAPE)of 1.09%.For advance rate(v),the MAE was 3.4 mm/min,with a MAPE of 4.50%.The predicted thrust(F)and torque(T)values exhibited strong agreement with field measurements,with MAEs of 270 kN and 178 kN∙m,respectively.Field applications demonstrated a 30%reduction in parameter adjustment time compared to empirical methods.This work provides a robust solution for real-time TBM control,advancing intelligent tunneling in complex geological environments.
基金Key Research Project of Hubei Provincial Tobacco Company(027Y2022-006)Hubei Provincial Natural Science Foundation and Meteorological Innovation and Development Joint Foundation of China(2023AFD104,2022CFD132)+4 种基金Open Project Fund of China Meteorological Administration Basin Heavy Rainfall Key Laboratory(2023BHR-Y03)Open Re-search Fund of China Meteorological Administration/Ministry of Rural Agriculture Tobacco Meteorological Service Center(KYZX2023-08)National Natural Science Foundation of China(42105039)Basic Research Fund of WHIHR(202314)Open Research Topics of Key Open Laboratory of Hydro-Meteorology,China Meteorological Administration(23SWQXM018)。
文摘Based on rain gauge data during 2008-2021 from national meteorological observation stations,this study investigated the performance of the precipitation field from the 1-km-resolution version of the China Atmospheric Realtime Analysis(CARAS)over Hubei from the perspective of climatology,multiple-time scale variations,as well as fusion accuracy and detection capability at multiple temporal scales.The results show that CARAS precipitation can reproduce the spatial distribution patterns of climatological seasonal precipitation and rainy days well over the whole of Hubei compared with observational(OBS)precipitation,albeit deviations exist between CARAS and OBS in terms of magnitude.Moreover,high correlation and consistency between CARAS and OBS can be found in multiple-time scale variations over Hubei,with correlation coefficients of interannual,seasonal,and diurnal variation generally exceeding 0.85,0.98,and 0.95,respectively.Furthermore,CARAS has a relatively higher fusion accuracy in summer and winter,and stronger/weaker detection capability in spring/winter at a daily scale.However,the detection capability of CARAS at an hourly scale is weaker than that at a daily scale.With different precipitation intensity levels considered,CARAS daily precipitation shows relatively higher fusion accuracy in estimating moderate and heavy rain,and better detection capability in capturing no rain events.The variations of accuracy metrics and detection metrics under different precipitation intensities at an hourly scale generally resemble those at a daily scale.However,CARAS precipitation at an hourly scale shows a relatively lower fusion accuracy and weaker detection capability compared with that at a daily scale.This paper provides an insight into the characteristics of systematic deviations in CARAS precipitation over Hubei,which will benefit relevant applications of CARAS in meteorological operations over Hubei and the improvement of CARAS in the future.
