采用同源建模的方法构建了斑马鱼γ-氨基丁酸A型（GABAA）受体和果蝇RDL（resistance to dieldrin）受体跨膜区的三维结构，研究了氟虫腈在两个受体中作用位点的差异；采用分子对接和分子动力学方法，探讨了氟虫腈与斑马鱼GABAA受体和果...采用同源建模的方法构建了斑马鱼γ-氨基丁酸A型（GABAA）受体和果蝇RDL（resistance to dieldrin）受体跨膜区的三维结构，研究了氟虫腈在两个受体中作用位点的差异；采用分子对接和分子动力学方法，探讨了氟虫腈与斑马鱼GABAA受体和果蝇RDL受体的结合模式，并比较了氟虫腈与两个受体作用的差异性。结果表明：斑马鱼 GABAA受体和氟虫腈作用位点的结构与果蝇RDL受体和氟虫腈作用位点的结构存在一定的差异，果蝇 RDL 受体中的 Ala301对应斑马鱼GABAA受体α1亚基中的Val284和γ2亚基中的Ser306，氨基酸构象的差异较大；氟虫腈与斑马鱼GABAA受体的结合位点靠近胞内区一端，而与果蝇RDL受体的结合位点则位于受体第二跨膜区的Ala301～Leu308区域内。复合物分子动力学模拟结果表明，在模拟过程中，两个受体与氟虫腈复合物体系的势能可很快达到平衡状态。斑马鱼GABAA受体与氟虫腈之间形成4个氢键，其中概率大于60％的氢键有2个；而尽管果蝇RDL受体与氟虫腈形成了6个氢键，但只有1个氢键的概率大于50％，其复合物结合的稳定性比前者低。展开更多
The phenylpyrazole insecticide fipronil blocks resistance to dieldrin(RDL)γ-aminobutyric acid(GABA)receptors in insects,thereby impairing inhibitory neurotransmission.Some insect species,such as the diamondback moth(...The phenylpyrazole insecticide fipronil blocks resistance to dieldrin(RDL)γ-aminobutyric acid(GABA)receptors in insects,thereby impairing inhibitory neurotransmission.Some insect species,such as the diamondback moth(Plutella xylostella),possess more than one Rdl gene.The involvement of multiple Rdls in fipronil toxicity and resistance remains largely unknown.In this study,we investigated the roles of two Rdl genes,PxRdl1 and PxRdl2,in P.xylostella fipronil action.In Xenopus oocytes,RvRDL2 receptors were 40 times less sensitive to fipronil than P.vRDL1.P.vRDL2 receptors were also less sensitive to GABA compared with PxRDL1.Knockout of the fipronil-sensitive PxRdll reduced the fipronil potency 10-fold,whereas knockout of the fipronil-resistant PxRdl2 enhanced the fipronil potency 4.4-fold.Furthermore,in two fipronil-resistant diamondback moth field populations,PxRdl2 expression was elevated 3.7-and 4.1-fold compared with a susceptible strain,whereas PxRdl1 expression was comparable among the resistant and susceptible strains.Collectively,our results indicate antagonistic effects of PxRDLl and PxRDL2 on fipronil action in vivo and suggest that enhanced expression of fipronil-resistant PxRdl1 is potentially a new mechanism of fipronil resistance in insects.展开更多
系统级封装SiP(System in a Package)已成为后摩尔时代延续摩尔定律的主要技术路线,电子装备小型化和多功能化的推动,使其在领域具有广阔前景。描述了一种基于SiP技术小型化多核信号处理的实现方案,详细讲述了芯片级设计及封装的具体方...系统级封装SiP(System in a Package)已成为后摩尔时代延续摩尔定律的主要技术路线,电子装备小型化和多功能化的推动,使其在领域具有广阔前景。描述了一种基于SiP技术小型化多核信号处理的实现方案,详细讲述了芯片级设计及封装的具体方法和思路,提出了一种基于SiP技术的多核处理器微系统设计及实现。展开更多
基金supported by the National Key R&D Program of China(2018YFD0200300)the Project of Science and Technology in Guangdong Province(2018A030313188)+1 种基金the Natural Science Foundation of Guangdong Province(2017A030310490)the Research and Innovation Team of Key Technologies in Modern Agricultural Industry in Guangdong Province(2019KJ130).
文摘The phenylpyrazole insecticide fipronil blocks resistance to dieldrin(RDL)γ-aminobutyric acid(GABA)receptors in insects,thereby impairing inhibitory neurotransmission.Some insect species,such as the diamondback moth(Plutella xylostella),possess more than one Rdl gene.The involvement of multiple Rdls in fipronil toxicity and resistance remains largely unknown.In this study,we investigated the roles of two Rdl genes,PxRdl1 and PxRdl2,in P.xylostella fipronil action.In Xenopus oocytes,RvRDL2 receptors were 40 times less sensitive to fipronil than P.vRDL1.P.vRDL2 receptors were also less sensitive to GABA compared with PxRDL1.Knockout of the fipronil-sensitive PxRdll reduced the fipronil potency 10-fold,whereas knockout of the fipronil-resistant PxRdl2 enhanced the fipronil potency 4.4-fold.Furthermore,in two fipronil-resistant diamondback moth field populations,PxRdl2 expression was elevated 3.7-and 4.1-fold compared with a susceptible strain,whereas PxRdl1 expression was comparable among the resistant and susceptible strains.Collectively,our results indicate antagonistic effects of PxRDLl and PxRDL2 on fipronil action in vivo and suggest that enhanced expression of fipronil-resistant PxRdl1 is potentially a new mechanism of fipronil resistance in insects.
文摘系统级封装SiP(System in a Package)已成为后摩尔时代延续摩尔定律的主要技术路线,电子装备小型化和多功能化的推动,使其在领域具有广阔前景。描述了一种基于SiP技术小型化多核信号处理的实现方案,详细讲述了芯片级设计及封装的具体方法和思路,提出了一种基于SiP技术的多核处理器微系统设计及实现。
