Clubroot disease is a severe threat to Brassica crops globally,particularly in western Canada.Genetic resistance,achieved through pyramiding clubroot resistance(CR)genes with different modes of action,is the most impo...Clubroot disease is a severe threat to Brassica crops globally,particularly in western Canada.Genetic resistance,achieved through pyramiding clubroot resistance(CR)genes with different modes of action,is the most important strategy for managing the disease.However,studies on the CR gene functions are quite limited.In this study,we have conducted investigations into the temporal,structural,and interacting features of a newly cloned CR gene,Rcr1,using CRISPR/Cas9 technology.For temporal functionality,we developed a novel CRISPR/Cas9-based binary vector,pHHIGR-Hsp18.2,to deliver Rcr1 into a susceptible canola line(DH12075)and observed that early expression of Rcr1 is critical for conferring resistance.For structural functionality,several independent mutations in specific domains of Rcr1 resulted in loss-offunction,highlighting their importance for CR phenotype.In the study of the interacting features of Rcr1,a cysteine protease gene and its homologous allele in canola were successfully disrupted via CRISPR/Cas9 as an interacting component with Rcr1 protein,resulting in the conversion from clubroot resistant to susceptible in plants carrying intact Rcr1.These results indicated an indispensable role of these two cysteine proteases in Rcr1-mediated resistance response.This study,the first of its kind,provides valuable insights into the functionality of Rcr1.Further,the new vector p HHIGR-Hsp18.2 demonstrated an inducible feature on the removal of add-on traits,which should be useful for functional genomics and other similar research in brassica crops.展开更多
RCR(Rafael A ra nda、Ca r me Pigem a nd Ra m on Vilata)是著名的西班牙建筑师组合,三人荣获2017年普利兹克建筑奖。自1988年工作室成立,他们一直关注于建筑与生态环境的共生关系,同时致力于建筑及其周边环境的整合。该文在查阅相关...RCR(Rafael A ra nda、Ca r me Pigem a nd Ra m on Vilata)是著名的西班牙建筑师组合,三人荣获2017年普利兹克建筑奖。自1988年工作室成立,他们一直关注于建筑与生态环境的共生关系,同时致力于建筑及其周边环境的整合。该文在查阅相关文献资料的基础上,结合西班牙当地的文化特色和生态特征,以RCR及其设计作品为主要研究对象,从他们的地域性设计原则、人本性建筑体验和平衡性细节考量三方面,剖析RCR建筑创作中的生态性特征体现。展开更多
基金supported by the Genomics Initiative of Agriculture and Agri-Food Canada。
文摘Clubroot disease is a severe threat to Brassica crops globally,particularly in western Canada.Genetic resistance,achieved through pyramiding clubroot resistance(CR)genes with different modes of action,is the most important strategy for managing the disease.However,studies on the CR gene functions are quite limited.In this study,we have conducted investigations into the temporal,structural,and interacting features of a newly cloned CR gene,Rcr1,using CRISPR/Cas9 technology.For temporal functionality,we developed a novel CRISPR/Cas9-based binary vector,pHHIGR-Hsp18.2,to deliver Rcr1 into a susceptible canola line(DH12075)and observed that early expression of Rcr1 is critical for conferring resistance.For structural functionality,several independent mutations in specific domains of Rcr1 resulted in loss-offunction,highlighting their importance for CR phenotype.In the study of the interacting features of Rcr1,a cysteine protease gene and its homologous allele in canola were successfully disrupted via CRISPR/Cas9 as an interacting component with Rcr1 protein,resulting in the conversion from clubroot resistant to susceptible in plants carrying intact Rcr1.These results indicated an indispensable role of these two cysteine proteases in Rcr1-mediated resistance response.This study,the first of its kind,provides valuable insights into the functionality of Rcr1.Further,the new vector p HHIGR-Hsp18.2 demonstrated an inducible feature on the removal of add-on traits,which should be useful for functional genomics and other similar research in brassica crops.
文摘RCR(Rafael A ra nda、Ca r me Pigem a nd Ra m on Vilata)是著名的西班牙建筑师组合,三人荣获2017年普利兹克建筑奖。自1988年工作室成立,他们一直关注于建筑与生态环境的共生关系,同时致力于建筑及其周边环境的整合。该文在查阅相关文献资料的基础上,结合西班牙当地的文化特色和生态特征,以RCR及其设计作品为主要研究对象,从他们的地域性设计原则、人本性建筑体验和平衡性细节考量三方面,剖析RCR建筑创作中的生态性特征体现。
