RCC2(regulator of chromosome condensation 2),也被称为TD-60(telophase disc protein of 60 kD),是一种高度保守的核蛋白质,结构与RCC1类似。RCC1是Ras相关核蛋白(Ras-related nuclear protein,Ran)的鸟嘌呤交换因子(guanine nucleot...RCC2(regulator of chromosome condensation 2),也被称为TD-60(telophase disc protein of 60 kD),是一种高度保守的核蛋白质,结构与RCC1类似。RCC1是Ras相关核蛋白(Ras-related nuclear protein,Ran)的鸟嘌呤交换因子(guanine nucleotide exchange factor,GEF),对于细胞周期的正常进行非常重要[1]。类似的,RCC2也主要参与细胞周期的调控;而另一方面,RCC2还可与整合素(integrin)、皮质肌动蛋白(cortactin)等发生相互作用来调节细胞的迁移和细胞分裂[2]。展开更多
Cytokinesis is required for faithful division of cytoplasmic components and duplicated nuclei into two daughter cells.Midbody,a protein-dense organelle that forms at the intercellular bridge,is indispensable for succe...Cytokinesis is required for faithful division of cytoplasmic components and duplicated nuclei into two daughter cells.Midbody,a protein-dense organelle that forms at the intercellular bridge,is indispensable for success-ful cytokinesis.However,the regulatory mechanism of cytokinesis at the midbody still remains elusive.Here,we unveil a critical role for NudC-like protein 2(NudCL2),a co-chaperone of heat shock protein 90(Hsp90),in cytokinesis regulation by stabilizing regulator of chromosome condensation 2(RCC2)at the midbody in mam-malian cells.NudCL2 localizes at the midbody,and its downregulation results in cytokinesis failure,multinu-cleation,and midbody disorganization.Using iTRAQ-based quantitative proteomic analysis,we find that RCC2 levels are decreased in NudCL2 knockout(KO)cells.Moreover,Hsp90 forms a complex with NudCL2 to stabilize RCC2,which is essential for cytokinesis.RCC2 depletion mirrors phenotypes observed in NudCL2-downregulated cells.Importantly,ectopic expression of RCC2 rescues the cytokinesis defects induced by NudCL2 deletion,but not vice versa.Together,our data reveal the significance of the NudCL2/Hsp90/RCC2 pathway in cytokinesis at the midbody.展开更多
Coral reefs have tremendous benefits to both humans and marine biodiversity but are globally endangered. Traditionally, data collection of coral reefs was fieldwork based, which is time-consuming, costly and lacking i...Coral reefs have tremendous benefits to both humans and marine biodiversity but are globally endangered. Traditionally, data collection of coral reefs was fieldwork based, which is time-consuming, costly and lacking in full coverage. This study shows that optical imageries can be utilised to map coral reefs, by fusing visible bands and depth invariant index (DII) to classify benthic features. Overall accuracy of at least 68 percent is achieved. However, to overcome the challenge of cloud cover, which is common in coastal areas, microwave imagery is used to map the emergent coral reefs. Again, remote sensing proves to be reliable in estimation of sea surface temperature (SST) and extraction of water quality parameters through Case-2 Region CoastColour (C2RCC) processor. Thereafter, regression analysis is performed to evaluate the correlation of SST and water quality. It is concluded that higher spatial resolution can improve coral mapping and that there is a need for more research on correlation to its environment.展开更多
文摘RCC2(regulator of chromosome condensation 2),也被称为TD-60(telophase disc protein of 60 kD),是一种高度保守的核蛋白质,结构与RCC1类似。RCC1是Ras相关核蛋白(Ras-related nuclear protein,Ran)的鸟嘌呤交换因子(guanine nucleotide exchange factor,GEF),对于细胞周期的正常进行非常重要[1]。类似的,RCC2也主要参与细胞周期的调控;而另一方面,RCC2还可与整合素(integrin)、皮质肌动蛋白(cortactin)等发生相互作用来调节细胞的迁移和细胞分裂[2]。
基金supported by the National Natural Science Foundation of China(Nos.32070709,32270771,and U21A20197)the National Key Research and Development Program of China(Nos.2019YFA0802202)the Higher Education Discipline Innovation Project(also known as the111 Project)(Nos.B13026).
文摘Cytokinesis is required for faithful division of cytoplasmic components and duplicated nuclei into two daughter cells.Midbody,a protein-dense organelle that forms at the intercellular bridge,is indispensable for success-ful cytokinesis.However,the regulatory mechanism of cytokinesis at the midbody still remains elusive.Here,we unveil a critical role for NudC-like protein 2(NudCL2),a co-chaperone of heat shock protein 90(Hsp90),in cytokinesis regulation by stabilizing regulator of chromosome condensation 2(RCC2)at the midbody in mam-malian cells.NudCL2 localizes at the midbody,and its downregulation results in cytokinesis failure,multinu-cleation,and midbody disorganization.Using iTRAQ-based quantitative proteomic analysis,we find that RCC2 levels are decreased in NudCL2 knockout(KO)cells.Moreover,Hsp90 forms a complex with NudCL2 to stabilize RCC2,which is essential for cytokinesis.RCC2 depletion mirrors phenotypes observed in NudCL2-downregulated cells.Importantly,ectopic expression of RCC2 rescues the cytokinesis defects induced by NudCL2 deletion,but not vice versa.Together,our data reveal the significance of the NudCL2/Hsp90/RCC2 pathway in cytokinesis at the midbody.
文摘Coral reefs have tremendous benefits to both humans and marine biodiversity but are globally endangered. Traditionally, data collection of coral reefs was fieldwork based, which is time-consuming, costly and lacking in full coverage. This study shows that optical imageries can be utilised to map coral reefs, by fusing visible bands and depth invariant index (DII) to classify benthic features. Overall accuracy of at least 68 percent is achieved. However, to overcome the challenge of cloud cover, which is common in coastal areas, microwave imagery is used to map the emergent coral reefs. Again, remote sensing proves to be reliable in estimation of sea surface temperature (SST) and extraction of water quality parameters through Case-2 Region CoastColour (C2RCC) processor. Thereafter, regression analysis is performed to evaluate the correlation of SST and water quality. It is concluded that higher spatial resolution can improve coral mapping and that there is a need for more research on correlation to its environment.
