The retroviral vector （RCAS） has been widely used in avian system to study development and diseases, but is not suitable for mammals which do not produce the retrovirus receptor TVA. In this review, we trace the cur...The retroviral vector （RCAS） has been widely used in avian system to study development and diseases, but is not suitable for mammals which do not produce the retrovirus receptor TVA. In this review, we trace the current uses of RCAS-TVA approach in mammalian system with improved strategies, including generation of tv-a transgenic mice, use of soluble TVA receptor and retroviral receptor-ligand fusion proteins, improvement of RCAS vectors, and compare a series of mammalian models in variant studies of gene function, development, oncogenesis and gene therapy. All those studies demonstrate that the RCAS-TVA based mammalian models are powerful tools for understanding the mechanisms and target treating of human diseases.展开更多
目的检测表达在SiSo细胞上的受体结合肿瘤抗原(receptor-binding cancer antigen expressed on SiSo cells,RCASl)在不同肿瘤患者血清及组织中的表达水平,探讨其在肿瘤发生、发展中的作用机制.方法收集肿瘤患者85例(患者组)和健康体检者...目的检测表达在SiSo细胞上的受体结合肿瘤抗原(receptor-binding cancer antigen expressed on SiSo cells,RCASl)在不同肿瘤患者血清及组织中的表达水平,探讨其在肿瘤发生、发展中的作用机制.方法收集肿瘤患者85例(患者组)和健康体检者(对照组)98例,采用免疫组织化学染色方法观察肿瘤组织中RCAS1的表达情况,采用ELISA方法检测受检者血清中RCAS1水平,并对检测结果进行统计学分析.结果免疫组化结果显示RCAS1在肿瘤组织中呈粗颗粒状黄褐色反应产物.患者组RCAS1水平明显高于对照组,差异有统计学意义(P<0.01).鼻咽癌患者组织中RCAS1的阳性表达率为76.2%,且随着病情的加重,血清RCAS1的水平和阳性检出率均不断升高,各组间比较差异均有统计学意义(P<0.01,P<0.05).结论血清RCAS1水平检测可用于肿瘤患者的辅助诊断.展开更多
目的采用多种方法检测多种肿瘤细胞系中S iSo细胞表达的受体结合肿瘤抗原(RCAS1)的表达情况。方法采用实时定量聚合酶链反应(PCR)、Northern b lot检测、免疫组化法和酶联免疫吸附实验对10个人肿瘤细胞系及2个正常人细胞系RCAS1分子的...目的采用多种方法检测多种肿瘤细胞系中S iSo细胞表达的受体结合肿瘤抗原(RCAS1)的表达情况。方法采用实时定量聚合酶链反应(PCR)、Northern b lot检测、免疫组化法和酶联免疫吸附实验对10个人肿瘤细胞系及2个正常人细胞系RCAS1分子的表达进行了检测。结果实时定量PCR及Northern b lot结果显示,除了正常肝细胞LO2,各肿瘤细胞及正常人胚肾细胞293均见RCAS1 mRNA表达;免疫组化见各肿瘤细胞和293细胞的细胞膜和细胞浆中均有RCAS1蛋白,但在LO2细胞中未见RCAS1蛋白;除了HuH-7和LO2细胞,其余10个细胞培养上清液中的RCAS1蛋白量均明显高于DMEM全培液(P<0.001)。结论RCAS1广泛表达于多种肿瘤细胞系中。展开更多
文摘The retroviral vector （RCAS） has been widely used in avian system to study development and diseases, but is not suitable for mammals which do not produce the retrovirus receptor TVA. In this review, we trace the current uses of RCAS-TVA approach in mammalian system with improved strategies, including generation of tv-a transgenic mice, use of soluble TVA receptor and retroviral receptor-ligand fusion proteins, improvement of RCAS vectors, and compare a series of mammalian models in variant studies of gene function, development, oncogenesis and gene therapy. All those studies demonstrate that the RCAS-TVA based mammalian models are powerful tools for understanding the mechanisms and target treating of human diseases.
文摘目的检测表达在SiSo细胞上的受体结合肿瘤抗原(receptor-binding cancer antigen expressed on SiSo cells,RCASl)在不同肿瘤患者血清及组织中的表达水平,探讨其在肿瘤发生、发展中的作用机制.方法收集肿瘤患者85例(患者组)和健康体检者(对照组)98例,采用免疫组织化学染色方法观察肿瘤组织中RCAS1的表达情况,采用ELISA方法检测受检者血清中RCAS1水平,并对检测结果进行统计学分析.结果免疫组化结果显示RCAS1在肿瘤组织中呈粗颗粒状黄褐色反应产物.患者组RCAS1水平明显高于对照组,差异有统计学意义(P<0.01).鼻咽癌患者组织中RCAS1的阳性表达率为76.2%,且随着病情的加重,血清RCAS1的水平和阳性检出率均不断升高,各组间比较差异均有统计学意义(P<0.01,P<0.05).结论血清RCAS1水平检测可用于肿瘤患者的辅助诊断.
文摘目的采用多种方法检测多种肿瘤细胞系中S iSo细胞表达的受体结合肿瘤抗原(RCAS1)的表达情况。方法采用实时定量聚合酶链反应(PCR)、Northern b lot检测、免疫组化法和酶联免疫吸附实验对10个人肿瘤细胞系及2个正常人细胞系RCAS1分子的表达进行了检测。结果实时定量PCR及Northern b lot结果显示,除了正常肝细胞LO2,各肿瘤细胞及正常人胚肾细胞293均见RCAS1 mRNA表达;免疫组化见各肿瘤细胞和293细胞的细胞膜和细胞浆中均有RCAS1蛋白,但在LO2细胞中未见RCAS1蛋白;除了HuH-7和LO2细胞,其余10个细胞培养上清液中的RCAS1蛋白量均明显高于DMEM全培液(P<0.001)。结论RCAS1广泛表达于多种肿瘤细胞系中。
