In recent decades,RNA binding motif(RBM)proteins have been widespread concerned by researchers.Among them,RBM5 is considered as a potential tumor suppressor gene in HCC.RBM10,also belonging to the RBM family,have simi...In recent decades,RNA binding motif(RBM)proteins have been widespread concerned by researchers.Among them,RBM5 is considered as a potential tumor suppressor gene in HCC.RBM10,also belonging to the RBM family,have similar structure and high homology with RBM5,indicating its potential as potential tumor suppressor genes.However,the role of RBM10 in tumors is controversial.The purpose of this study was to analyze the expression correlation and functional relationship of miR-21 and RBM10 in human hepatocellular carcinoma(HCC)tissues and corresponding tumor cells.Bioinformatics analysis showed that miR-21 and RBM10 were both highly expressed in HCC;similarly,the expression levels of miR-21 and RBM10 in HCC cells were significantly higher than those in normal hepatocytes.There was a positive correlation between miR-21 and RBM10.Furthermore,knockdown of RBM10 inhibited the proliferation,migration and invasion of SNU-398 cells,and simultaneous overexpression of miR-21 attenuated this inhibitory effect.Meanwhile,overexpressing RBM10 could promote the proliferation,migration and invasion of Hep G2 cells;while the stability of miR-21 could be reduced by knocking down RBM10.On the whole,the findings of this study indicate that RBM10 is involved in regulating the function and activity of human HCC cells by affecting the stability of miR-21.RBM10 cannot be simply summarized as a tumor suppressor or oncogene.Further studies are needed to clarify the role of RBM10 in tumors.展开更多
基金supported by National Natural Science Foundation of China,No.31970566.
文摘In recent decades,RNA binding motif(RBM)proteins have been widespread concerned by researchers.Among them,RBM5 is considered as a potential tumor suppressor gene in HCC.RBM10,also belonging to the RBM family,have similar structure and high homology with RBM5,indicating its potential as potential tumor suppressor genes.However,the role of RBM10 in tumors is controversial.The purpose of this study was to analyze the expression correlation and functional relationship of miR-21 and RBM10 in human hepatocellular carcinoma(HCC)tissues and corresponding tumor cells.Bioinformatics analysis showed that miR-21 and RBM10 were both highly expressed in HCC;similarly,the expression levels of miR-21 and RBM10 in HCC cells were significantly higher than those in normal hepatocytes.There was a positive correlation between miR-21 and RBM10.Furthermore,knockdown of RBM10 inhibited the proliferation,migration and invasion of SNU-398 cells,and simultaneous overexpression of miR-21 attenuated this inhibitory effect.Meanwhile,overexpressing RBM10 could promote the proliferation,migration and invasion of Hep G2 cells;while the stability of miR-21 could be reduced by knocking down RBM10.On the whole,the findings of this study indicate that RBM10 is involved in regulating the function and activity of human HCC cells by affecting the stability of miR-21.RBM10 cannot be simply summarized as a tumor suppressor or oncogene.Further studies are needed to clarify the role of RBM10 in tumors.
