Dear Editor,Haploid induction by outcrossing with inducers greatly simplifies and accelerates both classical(e.g.,doubled haploid,DH)and modern(e.g.,Hi-Edit,cytoplasmic male sterility[CMS]swap)breeding strategies(Kell...Dear Editor,Haploid induction by outcrossing with inducers greatly simplifies and accelerates both classical(e.g.,doubled haploid,DH)and modern(e.g.,Hi-Edit,cytoplasmic male sterility[CMS]swap)breeding strategies(Kelliher et al.,2019;Han et al.,2024).A promising approach to developing high-efficiency haploid inducers in plants is the manipulation of centromere-specific histone H3(CENH3),a key protein essential for faithful chromosome segregation(Ravi and Chan 2010).However,CENH3 manipulation presents a dual challenge:although it can hamper the inducer’s chromosome segregation in embryos to induce haploids,it also risks compromising cellular and developmental processes,leading to severe defects and limiting successful development of CENH3-based haploid inducers(Wang et al.,2023).For instance,rough knockdown of CENH3 using p35S-driven RNAi causes developmental abnormalities without achieving haploid induction,even at 30◦C(Lermontova et al.,2011;Ahmadli et al.,2023).Thus,achieving a balance between haploid induction efficiency and plant health remains a significant challenge.Here,we propose that stacking moderate modifications of CENH3,rather than completely disrupting it,may serve as a more effective strategy for creating functional haploid inducers.展开更多
基金supported by the National Natural Science Foundation of China under grant 32471538(to J.L.)the Postdoctoral Fellowship Program of CPSF under grant GZB20240186(to Z.W.)+2 种基金the China Postdoctoral Science Foundation under grant 2024M760711(to Z.W.)the Innovative Research Projects for Graduate Students in Hainan Province under grant Qhyb2022-66(to H.Y.)the Hainan University Startup Fund under grant RZ2100003224(to J.L.).
文摘Dear Editor,Haploid induction by outcrossing with inducers greatly simplifies and accelerates both classical(e.g.,doubled haploid,DH)and modern(e.g.,Hi-Edit,cytoplasmic male sterility[CMS]swap)breeding strategies(Kelliher et al.,2019;Han et al.,2024).A promising approach to developing high-efficiency haploid inducers in plants is the manipulation of centromere-specific histone H3(CENH3),a key protein essential for faithful chromosome segregation(Ravi and Chan 2010).However,CENH3 manipulation presents a dual challenge:although it can hamper the inducer’s chromosome segregation in embryos to induce haploids,it also risks compromising cellular and developmental processes,leading to severe defects and limiting successful development of CENH3-based haploid inducers(Wang et al.,2023).For instance,rough knockdown of CENH3 using p35S-driven RNAi causes developmental abnormalities without achieving haploid induction,even at 30◦C(Lermontova et al.,2011;Ahmadli et al.,2023).Thus,achieving a balance between haploid induction efficiency and plant health remains a significant challenge.Here,we propose that stacking moderate modifications of CENH3,rather than completely disrupting it,may serve as a more effective strategy for creating functional haploid inducers.
