目的:拟采用超微血管成像技术(superb microvascular imaging,SMI)和超声造影(contrast-enhanced ultrasound,CEUS)相结合的方法,对甲状腺4a及4b级结节进行TI-RADS(thyroid imaging reporting and data system)分级。方法:对27例超声造...目的:拟采用超微血管成像技术(superb microvascular imaging,SMI)和超声造影(contrast-enhanced ultrasound,CEUS)相结合的方法,对甲状腺4a及4b级结节进行TI-RADS(thyroid imaging reporting and data system)分级。方法:对27例超声造影证实的甲状腺结节进行回顾性分析,术前分别行2 D灰阶超声、超微血管显像和超声造影,并对其进行分组、定量计分,得到各组的工作特性曲线(receiver operating characteristic,ROC)。结果:(1)27例行超声造影检查的甲状腺结节中:TI-RADS分级联合超微血管成像与TI-RADS分级联合超声造影曲线下面积相比较(Z=-0.206,P=0.175),诊断效能差异无统计学意义(P>0.05),表明SMI与CEUS诊断效能近似;(2)27例行超声造影检查的甲状腺结节中:TI-RADS分级联合超微血管成像和超声造影与TI-RADS分级曲线下面积相比较(Z=-1.242,P=0.011),诊断效能差异有统计学意义(P<0.05)。结论:TI-RADS分级联合超微血管成像结合超声造影能够提高甲状腺结节诊断的准确性,能够为临床诊治提供较为准确指导;超微血管成像与超声造影在鉴别诊断甲状腺结节良恶性方面具有较高一致性。展开更多
UBL-UBA protein functions as a shuttle factor in the 26S ubiquitin degradation pathway,playing a critical role in plant growth and development,and responding to various biotic and abiotic stresses.Although RAD23,a typ...UBL-UBA protein functions as a shuttle factor in the 26S ubiquitin degradation pathway,playing a critical role in plant growth and development,and responding to various biotic and abiotic stresses.Although RAD23,a type of UBL-UBA protein,has been extensively studied in several plants,there is currently no comprehensive analysis available for kiwifruit(Actinidia chinensis).In this study,we identified six AcRAD23 genes in kiwifruit and further analyzed their phylogenetic relationships,gene structure,conserved motif composition and cis-acting element in the promoter.Subcellular localization experiments revealed that all AcRAD23 were localized in the nucleus and the cell membranes.Quantitative real-time PCR(qRT-PCR)analysis demonstrated differential expression patterns of these Ac RAD23 genes across different tissues and under various stress conditions(drought,waterlogging,salt stress,etc.),with AcRAD23D1 showing the highest responsiveness to abiotic stress.Additionally,we investigated the biological function of Ac RAD23D1 using VIGS-mediated gene silencing methods under drought stress conditions.Suppression of AcRAD23D1 expression resulted in reduced relative water content(RWC)but increased malondialdehyde(MDA)content and relative electrolyte leakage(REL)levels in D1-VIGS lines compared to control lines.Furthermore,D1-VIGS lines exhibited a higher accumulation of reactive oxygen species(ROS)along with decreased superoxide dismutase(SOD)and peroxidase(POD)enzyme activities.These findings suggest that AcRAD23D1 may play a positive role in regulating kiwifruit's response to drought stress.Our results provide new insights into the potential involvement of AcRAD23 under abiotic stress conditions while offering a theoretical foundation for understanding the molecular mechanisms underlying kiwifruit's adaptation to stresses.展开更多
基金financially supported by the National Natural Science Foundation of China(32472679)the Sichuan Science and Technology Department Projects(2023ZHCG0098,2024JDRC0011)+2 种基金the Chengdu Science and Technology Department Project,China(2024-YF0500408-SN)the Postdoctoral Fellowship Program of China Postdoctoral Science Foundation(GZC20231871)the Special Project for the Double Support Plan of Discipline Construction at Sichuan Agricultural University,China(2024ZYTS021)。
文摘UBL-UBA protein functions as a shuttle factor in the 26S ubiquitin degradation pathway,playing a critical role in plant growth and development,and responding to various biotic and abiotic stresses.Although RAD23,a type of UBL-UBA protein,has been extensively studied in several plants,there is currently no comprehensive analysis available for kiwifruit(Actinidia chinensis).In this study,we identified six AcRAD23 genes in kiwifruit and further analyzed their phylogenetic relationships,gene structure,conserved motif composition and cis-acting element in the promoter.Subcellular localization experiments revealed that all AcRAD23 were localized in the nucleus and the cell membranes.Quantitative real-time PCR(qRT-PCR)analysis demonstrated differential expression patterns of these Ac RAD23 genes across different tissues and under various stress conditions(drought,waterlogging,salt stress,etc.),with AcRAD23D1 showing the highest responsiveness to abiotic stress.Additionally,we investigated the biological function of Ac RAD23D1 using VIGS-mediated gene silencing methods under drought stress conditions.Suppression of AcRAD23D1 expression resulted in reduced relative water content(RWC)but increased malondialdehyde(MDA)content and relative electrolyte leakage(REL)levels in D1-VIGS lines compared to control lines.Furthermore,D1-VIGS lines exhibited a higher accumulation of reactive oxygen species(ROS)along with decreased superoxide dismutase(SOD)and peroxidase(POD)enzyme activities.These findings suggest that AcRAD23D1 may play a positive role in regulating kiwifruit's response to drought stress.Our results provide new insights into the potential involvement of AcRAD23 under abiotic stress conditions while offering a theoretical foundation for understanding the molecular mechanisms underlying kiwifruit's adaptation to stresses.
