Objective] This study aimed to investigate the effects of glycidamide （GA） on growth and progesterone biosynthesis of rat R2C Leydig cel s cultured in vitro. [Method] The R2C Leydig cel s were treated with GA with c...Objective] This study aimed to investigate the effects of glycidamide （GA） on growth and progesterone biosynthesis of rat R2C Leydig cel s cultured in vitro. [Method] The R2C Leydig cel s were treated with GA with concentrations of 0.25, 0.5, 0.75, 1, 2, 4 and 6 mmol/L respectively for 48 h. The IC25, IC50 and IC75 values of GA were al detected by MTT assay. The Leydig cel s were treated with GA at concentrations of IC25, IC50 and IC75 respectively for 48 h, and then the morphology of Leydig cel s was observed. After the Leydig cel s were treated with GA for 4 h, the cel ular DNA damage was measured by the comet assay technique; and after the Leydig cel s were with treated with GA for 24 h, the progesterone biosynthesis amount was detected by radioimmunoassay （RIA）. [Result] GA could inhibit the via-bility of R2C Leydig cel s, and its IC25, IC50 and IC75 were 0.635, 0.872 and 1.198 mmol/L, respectively. The GA at concentrations of IC25, IC50 and IC75 affected the growth and morphology of rat R2C Leydig cel s in varying degrees. The 4-h treat-ment of GA could significantly damage the DNA of R2C Leydig cel s, and the 24-h treatment of GA at concentrations of IC25, IC50 and IC75 al reduced the progesterone biosynthesis amount. [Conclusion] GA could inhibit the growth and progesterone biosynthesis of rat R2C Leydig cel s.展开更多
Most current biped robots are equipped with two feet arranged in the right and left which inspired by the human body system. Different from the existing configurations, a novel biped robot with inner and outer feet ba...Most current biped robots are equipped with two feet arranged in the right and left which inspired by the human body system. Different from the existing configurations, a novel biped robot with inner and outer feet based on a spatial six-bar 4R2C（R and C denote revolute and cylindric joints, respectively） mechanism is proposed. It can move along a line or a curve by three walking modes that are dwell adjustment mode, limit position adjustment mode and any position adjustment mode. Kinematic, gait planning and stability analyses are performed respectively, and a prototype is developed. Lastly, a potential application is considered and two manipulating modes（sphere and cylinder manipulating modes） are carried out. This interesting mechanism feathering its single dosed-chain structure and unique work performance is expected to motivate the configuration creation of biped robots.展开更多
基金Supported by Training Program for Outstanding Young Teachers in Higher Education Institutions,Guangdong Province(Yq2013024)Program for New Century Excellent Talents in University of Ministry of Education of ChinaNational Natural Science Foundation of China(31201402,31201340)~~
文摘Objective] This study aimed to investigate the effects of glycidamide （GA） on growth and progesterone biosynthesis of rat R2C Leydig cel s cultured in vitro. [Method] The R2C Leydig cel s were treated with GA with concentrations of 0.25, 0.5, 0.75, 1, 2, 4 and 6 mmol/L respectively for 48 h. The IC25, IC50 and IC75 values of GA were al detected by MTT assay. The Leydig cel s were treated with GA at concentrations of IC25, IC50 and IC75 respectively for 48 h, and then the morphology of Leydig cel s was observed. After the Leydig cel s were treated with GA for 4 h, the cel ular DNA damage was measured by the comet assay technique; and after the Leydig cel s were with treated with GA for 24 h, the progesterone biosynthesis amount was detected by radioimmunoassay （RIA）. [Result] GA could inhibit the via-bility of R2C Leydig cel s, and its IC25, IC50 and IC75 were 0.635, 0.872 and 1.198 mmol/L, respectively. The GA at concentrations of IC25, IC50 and IC75 affected the growth and morphology of rat R2C Leydig cel s in varying degrees. The 4-h treat-ment of GA could significantly damage the DNA of R2C Leydig cel s, and the 24-h treatment of GA at concentrations of IC25, IC50 and IC75 al reduced the progesterone biosynthesis amount. [Conclusion] GA could inhibit the growth and progesterone biosynthesis of rat R2C Leydig cel s.
基金Supported by National Natural Science Foundation of China(Grant Nos.51175030,51505022)Foundation of Talents of Beijing Jiaotong University,China(Grant No.2015RC047)+1 种基金China Postdoctoral Science Foundation(Grant No.2013M531168)the Specialized Research Fund for the Doctoral Program of Higher Education,China(Grant No.20130009110030)
文摘Most current biped robots are equipped with two feet arranged in the right and left which inspired by the human body system. Different from the existing configurations, a novel biped robot with inner and outer feet based on a spatial six-bar 4R2C（R and C denote revolute and cylindric joints, respectively） mechanism is proposed. It can move along a line or a curve by three walking modes that are dwell adjustment mode, limit position adjustment mode and any position adjustment mode. Kinematic, gait planning and stability analyses are performed respectively, and a prototype is developed. Lastly, a potential application is considered and two manipulating modes（sphere and cylinder manipulating modes） are carried out. This interesting mechanism feathering its single dosed-chain structure and unique work performance is expected to motivate the configuration creation of biped robots.
