目的通过磁共振R2*mapping技术示踪及动态监测NSCLC裸鼠模型给药后不同时间点R2*值的变化趋势,阐明R2*mapping在评价F/A-PLGA@DOX/SPIO纳米载药体系在肿瘤成像中的应用价值及影像学基础。方法成功构建NSCLC裸鼠移植瘤模型24只,随机分为G...目的通过磁共振R2*mapping技术示踪及动态监测NSCLC裸鼠模型给药后不同时间点R2*值的变化趋势,阐明R2*mapping在评价F/A-PLGA@DOX/SPIO纳米载药体系在肿瘤成像中的应用价值及影像学基础。方法成功构建NSCLC裸鼠移植瘤模型24只,随机分为G1、G2两组,每组12只。分别尾静脉注射单纯SPIO以及F/A-PLGA@DOX/SPIO纳米载药体系,并采用1.5 T MR和动物专用小鼠线圈对建模成功荷瘤裸鼠皮下移植瘤模型进行R2*扫描获得肿瘤区域R2*值。实验结束后全部肿瘤组织及肝脏、脾脏普鲁士蓝染色。结果G2组每个时间点的R2*值均高于G1组(P<0.05)。相对于G1组,G2组肿瘤R2*值在早期上升趋势明显,且在给药后60h时明显高于G1组(P<0.05)。G1组排泄期较G2组明显提前。普鲁士蓝染色结果显示,G2组肿瘤的蓝色铁沉积高于SPIO组,而肝脏、脾脏组织的蓝色铁沉积低于G1。结论R2*值与肿瘤组织内的SPIO含量存在较高敏感性,R2*mapping技术提供了一种SPIO的肿瘤示踪成像方法。展开更多
Brain iron deposition has been proposed to play an important role in the pathophysiology of Alzheimer disease（AD）.The aim of this study was to investigate the correlation of brain iron accumulation with the severity...Brain iron deposition has been proposed to play an important role in the pathophysiology of Alzheimer disease（AD）.The aim of this study was to investigate the correlation of brain iron accumulation with the severity of cognitive impairment in patients with AD by using quantitative MR relaxation rate R2＇ measurements.Fifteen patients with AD,15 age-and sex-matched healthy controls,and 30 healthy volunteers underwent 1.5T MR multi-echo T2 mapping and T2＊ mapping for the measurement of transverse relaxation rate R2＇（R2＇=R2＊-R2）.We statistically analyzed the R2＇ and iron concentrations of bilateral hippocampus（HP）,parietal cortex（PC）,frontal white matter（FWM）,putamen（PU）,caudate nucleus（CN）,thalamus（TH）,red nucleus（RN）,substantia nigra（SN）,and dentate nucleus（DN） of the cerebellum for the correlation with the severity of dementia.Two-tailed t-test,Student-Newman-Keuls test（ANOVA） and linear correlation test were used for statistical analysis.In 30 healthy volunteers,the R2＇ values of bilateral SN,RN,PU,CN,globus pallidus（GP）,TH,and FWM were measured.The correlation with the postmortem iron concentration in normal adults was analyzed in order to establish a formula on the relationship between regional R2＇ and brain iron concentration.The iron concentration of regions of interest（ROI） in AD patients and controls was calculated by this formula and its correlation with the severity of AD was analyzed.Regional R2＇ was positively correlated with regional brain iron concentration in normal adults（r=0.977,P0.01）.Iron concentrations in bilateral HP,PC,PU,CN,and DN of patients with AD were significantly higher than those of the controls（P0.05）;Moreover,the brain iron concentrations,especially in parietal cortex and hippocampus at the early stage of AD,were positively correlated with the severity of patients＇ cognitive impairment（P0.05）.The higher the R2＇ and iron concentrations were,the more severe the cognitive impairment was.Regional R2＇ and iron concentration in parietal cortex and hippocampus were positively correlated with the severity of AD patients＇ cognitive impairment,indicating that it may be used as a biomarker to evaluate the progression of AD.展开更多
目的基于T2^(*)mapping定量分析业余马拉松运动员足踝部关节软骨的T2^(*)值,并分析其与性别、年龄、身体质量指数(body mass index,BMI)、跑龄、跑量之间的相关性。材料与方法于2023年7月份至2023年9月份招募重庆市长跑运动爱好者48名,...目的基于T2^(*)mapping定量分析业余马拉松运动员足踝部关节软骨的T2^(*)值,并分析其与性别、年龄、身体质量指数(body mass index,BMI)、跑龄、跑量之间的相关性。材料与方法于2023年7月份至2023年9月份招募重庆市长跑运动爱好者48名,其中跑量<300 km/月的36例(中低跑量组),跑量≥300 km/月的12例(高跑量组)。所有受试者均进行单侧无症状踝关节的MRI扫描,扫描序列包括T2^(*)mapping多回波自旋回波(spin echo,SE)序列矢状位、质子密度加权成像脂肪抑制(proton density-weighted imaging fat-saturated,PDWI-FS)序列矢状位、冠状位、横轴位以及T1加权脂肪抑制成像(T1-weighted imaging fat-saturated,T1WI-FS)序列横轴位。沿关节软骨轮廓边缘勾画距骨穹窿、跟骰关节跟骨面、骰骨面及后距下关节跟骨面、距骨面软骨作为感兴趣区(region of interest,ROI),获得相应的T2^(*)值。采用线性回归分析软骨T2^(*)值与年龄、BMI、跑龄的相关性,采用独立样本t检验分析不同跑量及不同性别间的软骨T2^(*)值差异。结果(1)距骨穹窿、跟骰关节跟骨面及骰骨面、后距下关节跟骨面及距骨面软骨T2^(*)值在性别上的差异均具有统计学意义(P=0.001、P<0.001、P=0.002、P=0.008、P=0.004);(2)高跑量组的距骨穹窿、后距下关节跟骨面软骨T2^(*)值高于中低跑量组(P=0.014、0.023),不同跑量的跟骰关节跟骨面及骰骨面、后距下关节距骨面软骨T2^(*)值的差异均无统计学意义(P=0.987、0.072、0.724);(3)距骨穹窿、跟骰关节跟骨面及骰骨面、后距下关节跟骨面、距骨面软骨T2^(*)值均与BMI呈正相关(r=0.376、0.384、0.300、0.422、0.455,P=0.005、0.004、0.019、0.001、0.001)。结论在业余马拉松运动员这一跑步群体中,与中低跑量相比,高跑量更有可能导致距骨穹窿、后距下关节跟骨面软骨损伤;而与较低的BMI相比,高BMI增加了距骨穹窿、跟骰关节跟骨面、骰骨面及后距下关节跟骨面、距骨面软骨损伤的风险。展开更多
文摘目的通过磁共振R2*mapping技术示踪及动态监测NSCLC裸鼠模型给药后不同时间点R2*值的变化趋势,阐明R2*mapping在评价F/A-PLGA@DOX/SPIO纳米载药体系在肿瘤成像中的应用价值及影像学基础。方法成功构建NSCLC裸鼠移植瘤模型24只,随机分为G1、G2两组,每组12只。分别尾静脉注射单纯SPIO以及F/A-PLGA@DOX/SPIO纳米载药体系,并采用1.5 T MR和动物专用小鼠线圈对建模成功荷瘤裸鼠皮下移植瘤模型进行R2*扫描获得肿瘤区域R2*值。实验结束后全部肿瘤组织及肝脏、脾脏普鲁士蓝染色。结果G2组每个时间点的R2*值均高于G1组(P<0.05)。相对于G1组,G2组肿瘤R2*值在早期上升趋势明显,且在给药后60h时明显高于G1组(P<0.05)。G1组排泄期较G2组明显提前。普鲁士蓝染色结果显示,G2组肿瘤的蓝色铁沉积高于SPIO组,而肝脏、脾脏组织的蓝色铁沉积低于G1。结论R2*值与肿瘤组织内的SPIO含量存在较高敏感性,R2*mapping技术提供了一种SPIO的肿瘤示踪成像方法。
基金supported by grants from the National Natural Science Foundation of China (No. 30870702 and No.30570531)863 Project of China (No. 2006AA02Z4A1)
文摘Brain iron deposition has been proposed to play an important role in the pathophysiology of Alzheimer disease（AD）.The aim of this study was to investigate the correlation of brain iron accumulation with the severity of cognitive impairment in patients with AD by using quantitative MR relaxation rate R2＇ measurements.Fifteen patients with AD,15 age-and sex-matched healthy controls,and 30 healthy volunteers underwent 1.5T MR multi-echo T2 mapping and T2＊ mapping for the measurement of transverse relaxation rate R2＇（R2＇=R2＊-R2）.We statistically analyzed the R2＇ and iron concentrations of bilateral hippocampus（HP）,parietal cortex（PC）,frontal white matter（FWM）,putamen（PU）,caudate nucleus（CN）,thalamus（TH）,red nucleus（RN）,substantia nigra（SN）,and dentate nucleus（DN） of the cerebellum for the correlation with the severity of dementia.Two-tailed t-test,Student-Newman-Keuls test（ANOVA） and linear correlation test were used for statistical analysis.In 30 healthy volunteers,the R2＇ values of bilateral SN,RN,PU,CN,globus pallidus（GP）,TH,and FWM were measured.The correlation with the postmortem iron concentration in normal adults was analyzed in order to establish a formula on the relationship between regional R2＇ and brain iron concentration.The iron concentration of regions of interest（ROI） in AD patients and controls was calculated by this formula and its correlation with the severity of AD was analyzed.Regional R2＇ was positively correlated with regional brain iron concentration in normal adults（r=0.977,P0.01）.Iron concentrations in bilateral HP,PC,PU,CN,and DN of patients with AD were significantly higher than those of the controls（P0.05）;Moreover,the brain iron concentrations,especially in parietal cortex and hippocampus at the early stage of AD,were positively correlated with the severity of patients＇ cognitive impairment（P0.05）.The higher the R2＇ and iron concentrations were,the more severe the cognitive impairment was.Regional R2＇ and iron concentration in parietal cortex and hippocampus were positively correlated with the severity of AD patients＇ cognitive impairment,indicating that it may be used as a biomarker to evaluate the progression of AD.
