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T-DNA插入水稻群体中卷叶突变体R1-A2的遗传分析 被引量:15
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作者 沈革志 王新其 +3 位作者 殷丽青 王江 李琳 张景六 《实验生物学报》 CSCD 北大核心 2003年第6期459-464,共6页
在根癌农杆菌介导的T-DNA(携带有除草剂Basta抗性基因bar和Ds因子)转化中花11水稻群体中,获得了一个叶片发生明显内卷的突变体R1-A。经过连续三代的分离鉴定,获得突变体的纯合株(R1-A2),并与中花11号进行杂交,在调查的36个F_1植株中,全... 在根癌农杆菌介导的T-DNA(携带有除草剂Basta抗性基因bar和Ds因子)转化中花11水稻群体中,获得了一个叶片发生明显内卷的突变体R1-A。经过连续三代的分离鉴定,获得突变体的纯合株(R1-A2),并与中花11号进行杂交,在调查的36个F_1植株中,全部表现为卷叶,并对Basta除草剂都表现为抗性。在852个F_2单株中,卷叶为645株,正常叶207株,卷叶和正常叶的比例为3:1,其中,卷叶株均对Basta表现抗性,正常叶株均对Basta表现敏感,表明卷叶性状和Basta抗性存在着共分离关系。用扩增Ds因子的引物,对F_2中45个卷叶抗性株进行PCR鉴定,都获得预期长度的Ds因子片段,进一步表明在这些卷叶的植株中都有T-DNA的插入;而30个正常叶敏感株都不能检测到Ds的特征片段。在以卷叶突变(R1-A2)为回交亲本的F_1B_1植株中,全部植株表现卷叶;在以中花11号为回交亲本的F_1B_1植株中,卷叶和正常叶植株的分离比为1:1。上述结果表明该卷叶突变是个显性突变,受一个基因所控制,且该基因的突变与T-DNA的插入有关。 展开更多
关键词 T-DNA插入 水稻 群体 卷叶突变体 r1-a2 遗传
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宫颈癌患者血清LncRNA SFTA1P和LncRNA UBE2R2-AS1表达水平与预后的关系 被引量:3
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作者 肖献花 郭丽娜 +4 位作者 呼慧军 张伟伟 冀娜娜 段敬梅 高翔 《安徽医学》 2025年第1期22-27,共6页
目的探究宫颈癌(CC)患者血清中长链非编码RNA(LncRNA)表面活性剂相关1假基因(SFTA1P)和UBE2R2-AS1表达水平及其与预后的关系。方法选取2018年4月至2021年4月邯郸市妇幼保健院收治的108例CC患者为CC组,100例同期健康体检者为健康组。用... 目的探究宫颈癌(CC)患者血清中长链非编码RNA(LncRNA)表面活性剂相关1假基因(SFTA1P)和UBE2R2-AS1表达水平及其与预后的关系。方法选取2018年4月至2021年4月邯郸市妇幼保健院收治的108例CC患者为CC组,100例同期健康体检者为健康组。用实时荧光定量PCR(qRT-PCR)法检测血清LncRNA SFTA1P和LncRNA UBE2R2-AS1水平;绘制受试者工作特征(ROC)曲线评估血清LncRNA SFTA1P和LncRNA UBE2R2-AS1对CC的诊断价值;Kaplan-Meier法分析血清LncRNA SFTA1P和LncRNA UBE2R2-AS1表达水平与CC患者预后的关系;Cox回归分析CC患者预后的影响因素。结果与健康组相比,CC患者血清LncRNA SFTA1P表达水平升高(P<0.05),LncRNA UBE2R2-AS1表达水平降低(P<0.05),且其水平与FIGO分期、淋巴结转移、肌层浸润深度有关(P<0.05);血清LncRNA SFTA1P和LncRNA UBE2R2-AS1单独及联合诊断CC发生的曲线下面积分别为0.863、0.817和0.915,联合优于单独诊断(Z_(二者联合-LncRNA SFTA1P)=3.057、P=0.002,Z_(二者联合-LncRNA UBE2R2-AS1)=3.564、P<0.001);血清LncRNA SFTA1P高表达组患者3年生存率低于低表达组患者(64.91%比80.39%,χ^(2)=4.443,P=0.035),LncRNA UBE2R2-AS1高表达组患者3年生存率高于低表达组患者(82.00%比63.79%,χ^(2)=5.938,P=0.015);FIGO分期Ⅱb~Ⅲc期、淋巴结转移、肌层浸润深度≥1/2、LncRNA SFTA1P表达水平升高及LncRNA UBE2R2-AS1表达水平降低均为CC患者预后的危险因素(P<0.05)。结论CC患者血清中LncRNA SFTA1P表达上调,LncRNA UBE2R2-AS1表达下调,其表达水平与肿瘤FIGO分期、淋巴结转移、肌层浸润深度有关,且与患者预后生存密切相关。 展开更多
关键词 宫颈癌 长链非编码rNA SFTA1P 长链非编码rNA UBE2r2-aS1 临床病理特征 预后
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Comparative study of intertrochanteric fracture fixation using proximal femoral nail with and without distal interlocking screws 被引量:4
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作者 Nadeem A Lil Vipul R Makwana +1 位作者 Tirth D Patel Arjav R Patel 《World Journal of Orthopedics》 2022年第3期267-277,共11页
BACKGROUND Intertrochanteric(IT)fracture is one of the most common fractures seen in an orthopaedic practice.Proximal femoral nailing(PFN)is a common modality of fixing IT femur fracture.We retrospectively studied whe... BACKGROUND Intertrochanteric(IT)fracture is one of the most common fractures seen in an orthopaedic practice.Proximal femoral nailing(PFN)is a common modality of fixing IT femur fracture.We retrospectively studied whether a PFN with two proximal lag screws can be done without distal interlocking screws in the 31-A1 and 31-A2 fracture patterns according to the Arbeitsgemeinschaft für Osteosynthesefragen/Orthopaedic Trauma Association(AO/OTA)guidelines for IT femur fractures.AIM To compare the outcomes of IT fractures(AO/OTA 31-A1 and 31-A2)treated by PFN with and without distal interlocking screws.METHODS We carried out a retrospective study of 140 patients in a tertiary care centre who had AO/OTA type 31-A1 and 31-A2 IT fractures.We divided the patients into two groups,in which one of the groups received distal interlocking screws(group 1)and the other group did not(group 2).The subjects were followed up for a mean period of 14 mo and assessed for radiological union time,fracture site collapse,mechanical stability of implant,and complications associated with the PFN with distal interlocking and without distal interlocking.Then,the results were compared.RESULTS PFN without distal interlocking screws has several advantages and gives better results over PFN with distal interlocking screws in the AO/OTA 31-A2 fracture pattern.However,similar results were observed in both groups with the fracture pattern AO/OTA 31-A1.In patients with fracture pattern AO/OTA 31-A2 treated by PFN without distal interlocking screws,there were minimal proximal lockrelated complications and no risk of distal interlock-related complications.The operative time,IITV radiation time and time to radiological union were reduced.These patients also had better rotational alignment of the proximal femur,and the anatomy of the proximal femur was well maintained.It was also noted that in the cases where distal interlocking was performed,there was a gradual decrease in neck shaft angle,which led to varus collapse and failure of bone-implant construct in 21.40%.CONCLUSION In fracture pattern AO/OTA 31-A2,PFN without distal interlocking had better results and less complications than PFN with distal interlocking. 展开更多
关键词 Intertrochanteric fracture Arbeitsgemeinschaft r Osteosynthesefra-gen/Orthopaedic Trauma Association 31-a1 and 31-a2 Proximal femoral nail Distal interlocking screws Without distal interlocking screws Outcome
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