背景与目的 肺腺癌易发生脑转移,患者预后极差。蛋白磷酸酶1调节亚基13样(protein phosphatase 1 regulatory subunit 13-like, PPP1R13L)基因编码的p53凋亡刺激蛋白家族抑制成员(inhibitor of apoptosis-stimulating protein of p53, i...背景与目的 肺腺癌易发生脑转移,患者预后极差。蛋白磷酸酶1调节亚基13样(protein phosphatase 1 regulatory subunit 13-like, PPP1R13L)基因编码的p53凋亡刺激蛋白家族抑制成员(inhibitor of apoptosis-stimulating protein of p53, iASPP)蛋白是p53通路的关键抑制因子,在多种肿瘤中促癌,但其在肺腺癌脑转移中的作用未知。本研究通过单细胞测序数据整合与临床样本分析,旨在分析肺腺癌脑转移患者的肿瘤微环境特征,并探讨PPP1R13L在脑转移组织中的表达情况及其临床意义。方法 收集2014年1月至2024年12月新疆医科大学附属肿瘤医院就诊的4例肺腺癌脑转移和2例少突胶质细胞瘤患者的脑组织进行单细胞测序,并结合公共数据库中4例肺腺癌样本和4例正常肺组织样本的单细胞测序数据解析肿瘤微环境;另收集此医院50例肺腺癌脑转移患者的临床资料及石蜡切片,采用免疫组化技术检测iASPP蛋白表达,分析其与临床特征及预后的关系。结果 相较于胶质瘤和肺腺癌组,肺腺癌脑转移组特异的上皮细胞主要富集于氧化磷酸化、细胞凋亡、缺氧和p53通路;PPP1R13L作为上调差异基因,在脑转移组特异上皮细胞亚群高表达;PPP1R13L阳性细胞与成纤维细胞交互作用显著,激活细胞-基质黏附相关通路,关键配体-受体对为I型胶原蛋白α1链-白细胞分化抗原44(collagen type I alpha 1 chain-cluster of differentiation 44, COL1A1-CD44)。临床数据分析显示,吸烟史(HR=2.543, 95%CI:1.159-5.583, P=0.020)和iASPP高表达(HR=3.351, 95%CI:1.310-8.575, P=0.012)为肺腺癌脑转移患者预后的独立危险因素。结论 本研究阐明了肺腺癌脑转移微环境中上皮细胞与成纤维细胞的相互作用,提示PPP1R13L可作为潜在预后标志物及治疗靶点,为肺腺癌脑转移的精准治疗提供依据。展开更多
[Objectives]To obtain a novel cultivar of Chuanminshen violaceum with robust growth,high yield,and stable genetic traits.[Methods]A systematic selection method was employed to conduct a multi-point testing and regiona...[Objectives]To obtain a novel cultivar of Chuanminshen violaceum with robust growth,high yield,and stable genetic traits.[Methods]A systematic selection method was employed to conduct a multi-point testing and regional production trial utilizing C.violaceum strain CMS1,which was sourced from a semi-wild population in Langzhong,Sichuan Province,as the experimental material.In contrast,C.violaceum CMS2,derived from a cultivated population in Langzhong,Sichuan Province,along with a mixed population of C.violaceum cultispecies from the same region,served as the control material.Through a comparative analysis of phenological periods,agronomic traits,yield,and quality,a novel cultivar,‘Chengming No.1’,was ultimately selected and developed based on its superior comprehensive evaluation.[Results]In the phenological period survey conducted as part of a two-year comparative study,the CMS1 strain exhibited a shorter growth cycle compared to others.Furthermore,the agronomic characteristics of the CMS1 strain were superior to those of both CMS2 and CK.The average yields of CMS1,CMS2,and CK in the 2019 cultivar comparison test and yield trial were 468.88,448.52,and 422.15 kg/667 m 2,respectively.This resulted in an average yield increase of 11.07%for CMS1 compared to CK and 6.25%for CMS2 compared to CK.The average yields of CMS1,CMS2,and CK in the 2020 cultivar comparison test and yield trial were 482.69,467.54,and 436.82 kg/667 m 2,respectively.CMS1 exhibited an average yield increase of 10.50%compared to CK,while CMS2 demonstrated an average yield increase of 7.03%relative to CK.Furthermore,the average yield of CMS1 per 667 m 2 achieved a statistically significant level compared to CK in both years of the study.In multiple-point comparison and yield trials conducted in 2019 and 2020,the CMS1 strain exhibited a total ash content of 15.30%,an acid-insoluble ash content of 1.30%,a moisture content of 10.80%,and water-soluble extract amounting to 11.40%.All of the indicators conformed to the criteria established by the Sichuan Standards for Chinese Medicinal Materials(2010 Edition).[Conclusions]The CMS1 strain successfully passed the field technical appraisal for the novel cultivar of C.violaceum in 2021.This cultivar is characterized by high yield,excellent quality,and stable traits.In 2022,it received validation from the Sichuan Provincial Committee for the Certification of Non-Staple Crop Varieties and was officially named‘Chengming No.1’(CRY 2022002).This cultivar demonstrates significant potential for widespread cultivation.展开更多
文摘背景与目的 肺腺癌易发生脑转移,患者预后极差。蛋白磷酸酶1调节亚基13样(protein phosphatase 1 regulatory subunit 13-like, PPP1R13L)基因编码的p53凋亡刺激蛋白家族抑制成员(inhibitor of apoptosis-stimulating protein of p53, iASPP)蛋白是p53通路的关键抑制因子,在多种肿瘤中促癌,但其在肺腺癌脑转移中的作用未知。本研究通过单细胞测序数据整合与临床样本分析,旨在分析肺腺癌脑转移患者的肿瘤微环境特征,并探讨PPP1R13L在脑转移组织中的表达情况及其临床意义。方法 收集2014年1月至2024年12月新疆医科大学附属肿瘤医院就诊的4例肺腺癌脑转移和2例少突胶质细胞瘤患者的脑组织进行单细胞测序,并结合公共数据库中4例肺腺癌样本和4例正常肺组织样本的单细胞测序数据解析肿瘤微环境;另收集此医院50例肺腺癌脑转移患者的临床资料及石蜡切片,采用免疫组化技术检测iASPP蛋白表达,分析其与临床特征及预后的关系。结果 相较于胶质瘤和肺腺癌组,肺腺癌脑转移组特异的上皮细胞主要富集于氧化磷酸化、细胞凋亡、缺氧和p53通路;PPP1R13L作为上调差异基因,在脑转移组特异上皮细胞亚群高表达;PPP1R13L阳性细胞与成纤维细胞交互作用显著,激活细胞-基质黏附相关通路,关键配体-受体对为I型胶原蛋白α1链-白细胞分化抗原44(collagen type I alpha 1 chain-cluster of differentiation 44, COL1A1-CD44)。临床数据分析显示,吸烟史(HR=2.543, 95%CI:1.159-5.583, P=0.020)和iASPP高表达(HR=3.351, 95%CI:1.310-8.575, P=0.012)为肺腺癌脑转移患者预后的独立危险因素。结论 本研究阐明了肺腺癌脑转移微环境中上皮细胞与成纤维细胞的相互作用,提示PPP1R13L可作为潜在预后标志物及治疗靶点,为肺腺癌脑转移的精准治疗提供依据。
基金Supported by Sichuan Innovation Team Project of China Agricultural Industry Research System(SCCXTD-2023-19)Key R&D Project of Sichuan Provincial Department of Science and Technology(2022YFS0592)Sichuan Provincial Science and Technology Innovation Seedling Project(MZGC20230119,MZGC20230126).
文摘[Objectives]To obtain a novel cultivar of Chuanminshen violaceum with robust growth,high yield,and stable genetic traits.[Methods]A systematic selection method was employed to conduct a multi-point testing and regional production trial utilizing C.violaceum strain CMS1,which was sourced from a semi-wild population in Langzhong,Sichuan Province,as the experimental material.In contrast,C.violaceum CMS2,derived from a cultivated population in Langzhong,Sichuan Province,along with a mixed population of C.violaceum cultispecies from the same region,served as the control material.Through a comparative analysis of phenological periods,agronomic traits,yield,and quality,a novel cultivar,‘Chengming No.1’,was ultimately selected and developed based on its superior comprehensive evaluation.[Results]In the phenological period survey conducted as part of a two-year comparative study,the CMS1 strain exhibited a shorter growth cycle compared to others.Furthermore,the agronomic characteristics of the CMS1 strain were superior to those of both CMS2 and CK.The average yields of CMS1,CMS2,and CK in the 2019 cultivar comparison test and yield trial were 468.88,448.52,and 422.15 kg/667 m 2,respectively.This resulted in an average yield increase of 11.07%for CMS1 compared to CK and 6.25%for CMS2 compared to CK.The average yields of CMS1,CMS2,and CK in the 2020 cultivar comparison test and yield trial were 482.69,467.54,and 436.82 kg/667 m 2,respectively.CMS1 exhibited an average yield increase of 10.50%compared to CK,while CMS2 demonstrated an average yield increase of 7.03%relative to CK.Furthermore,the average yield of CMS1 per 667 m 2 achieved a statistically significant level compared to CK in both years of the study.In multiple-point comparison and yield trials conducted in 2019 and 2020,the CMS1 strain exhibited a total ash content of 15.30%,an acid-insoluble ash content of 1.30%,a moisture content of 10.80%,and water-soluble extract amounting to 11.40%.All of the indicators conformed to the criteria established by the Sichuan Standards for Chinese Medicinal Materials(2010 Edition).[Conclusions]The CMS1 strain successfully passed the field technical appraisal for the novel cultivar of C.violaceum in 2021.This cultivar is characterized by high yield,excellent quality,and stable traits.In 2022,it received validation from the Sichuan Provincial Committee for the Certification of Non-Staple Crop Varieties and was officially named‘Chengming No.1’(CRY 2022002).This cultivar demonstrates significant potential for widespread cultivation.
