Objective:To evaluate the influence of extraction solvents on antioxidant and antimicrobial activities of the pulp and seed of Anisophyllea laurina R.Br.ex Sabine fruits.Methods:The antibacterial activities of pulp an...Objective:To evaluate the influence of extraction solvents on antioxidant and antimicrobial activities of the pulp and seed of Anisophyllea laurina R.Br.ex Sabine fruits.Methods:The antibacterial activities of pulp and seed extracts were tested by using disk diffusion method against eight bacterial strains and three fungal strains.Total phenolic,flavonoid,monomeric anthocyanin and tannin contents,and antioxidant activities were determined by spectrometric methods.Results:The antioxidant analysis of pulp extract revealed the strong radical scavenging capacity and total phenolic content(4 329.66 mg of gallic acid/100 g),while seed extract showed the high antioxidant activity and total tannin content(5 326.78 mg catechin equivalent/100 g).Antibacterial and antifungal activities of methanol and ethanol extracts exhibited potent growth inhibitory activity against Aeromonas hydrophila,Bacillus subtilis,Escherichia coli O157:H7,Pseudomonas aeruginosa,Salmonella Typhimurium and Staphylococcus aureus ATCC 6538 with minimum inhibitory concentration values ranged from 125 to 250 μg/mL.However,seed extract had the strongest potential activity against Aspergillus niger and Candida albicans with minimum inhibitory concentration value of 500 μg/mL compared to pulp extract.Conclusions:Our results therefore demonstrated that ethanol and methanol extractions were more efficient in extracting antioxidants and bioactive compound in pulp and seed.These results support that these plant extracts can be used for the treatment of bacterial infections.展开更多
A new guanidine derivative,complanatin has been isolated from the seeds of Chinese traditional medicine Astragalus complanatus R.Br..Its structure was established on the basis of spectral data and X-ray crystallograph...A new guanidine derivative,complanatin has been isolated from the seeds of Chinese traditional medicine Astragalus complanatus R.Br..Its structure was established on the basis of spectral data and X-ray crystallographic analysis to be N-[3-carboxypropyl]-N-[3-methyl-2-butenyl]guanidine.展开更多
[ Objective] The paper aimed to study the optimum extraction technology of pigments from petals of Matthiola incana（ L. ） R. Br. of differ- ent colors. [Method] Petal pigments of Aida variety （white violet）, Franc...[ Objective] The paper aimed to study the optimum extraction technology of pigments from petals of Matthiola incana（ L. ） R. Br. of differ- ent colors. [Method] Petal pigments of Aida variety （white violet）, Francesco variety （ red violet） and Arabella variety （ purple violet） were ana- lyzed by colorimetric reaction method and ultraviolet-visible spectrum method, and the optimum extraction technology of flavonoid compounds from petals of them was determined by single factor experiment and orthogonal experiment. [ Result] The optimum extraction conditions of white violet were as follows： with 95% ethanol ＋ 5% HCI as solvent, solid-liquid ratio （ 1 ： 40）, extraction time （3 h）, extraction temperature （65 ℃ ）. The opti- mum extraction conditions of red violet and purple violet were as follows： with acetone as solvent, solid-liquid ratio （1：40）, extraction time （3 h）, extraction temperature （65 ℃）. [ Conclusion] The study provided theoretical foundation for further development and exploitation of pigments from M. incana（ L. ） R. Br.展开更多
为明确影响鹅绒藤分布的主导环境因子及其生态阈值,量化不同时期其潜在适生区,借助MaxEnt模型与ArcGIS空间分析技术,融合鹅绒藤在我国的301个分布点位信息及生物气候数据,对其在2个过去时期[末次盛冰期(约22000 a前)和全新世中期(约600...为明确影响鹅绒藤分布的主导环境因子及其生态阈值,量化不同时期其潜在适生区,借助MaxEnt模型与ArcGIS空间分析技术,融合鹅绒藤在我国的301个分布点位信息及生物气候数据,对其在2个过去时期[末次盛冰期(约22000 a前)和全新世中期(约6000 a前)]、当前时期(1970—2000年)以及3个未来时期[2030s(2021—2040年)、2050s(2041—2060年)、2070s(2061—2080年)]的潜在适生区进行预测分析。结果表明,MaxEnt模型各时期受试者工作特征曲线下面积(Area under the curve,AUC)值均高于0.9,预测结果可靠。鹅绒藤的分布主要受年平均温度、温度季节性变异系数、降水量季节性变异系数和最湿月份降水量影响。当前时期下,鹅绒藤的总适生区面积达193.37675×10^(4)km^(2),其中高适生区面积为69.01563×10^(4)km^(2)。与当前时期相对比,鹅绒藤在末次盛冰期和全新世中期适生区扩张幅度较为显著。3个未来时期中,鹅绒藤在SSP370(区域竞争路径)气候变化情景下2070s适生区面积最大,在SSP126(可持续路径)气候变化情景下2030s适生区面积最小,但均比当前时期适生区面积有所扩张。综上,鹅绒藤在2个过去时期和3个未来时期不同气候情景下均呈现扩张趋势,反映了气候变化,尤其是碳循环所引起的温度变化对物种分布的阶段性影响。展开更多
基金Supported by National Natural Science Foundation of China(31271812)the National High Technology Research and Development Program of China(863 Program 2012AA022105C)
文摘Objective:To evaluate the influence of extraction solvents on antioxidant and antimicrobial activities of the pulp and seed of Anisophyllea laurina R.Br.ex Sabine fruits.Methods:The antibacterial activities of pulp and seed extracts were tested by using disk diffusion method against eight bacterial strains and three fungal strains.Total phenolic,flavonoid,monomeric anthocyanin and tannin contents,and antioxidant activities were determined by spectrometric methods.Results:The antioxidant analysis of pulp extract revealed the strong radical scavenging capacity and total phenolic content(4 329.66 mg of gallic acid/100 g),while seed extract showed the high antioxidant activity and total tannin content(5 326.78 mg catechin equivalent/100 g).Antibacterial and antifungal activities of methanol and ethanol extracts exhibited potent growth inhibitory activity against Aeromonas hydrophila,Bacillus subtilis,Escherichia coli O157:H7,Pseudomonas aeruginosa,Salmonella Typhimurium and Staphylococcus aureus ATCC 6538 with minimum inhibitory concentration values ranged from 125 to 250 μg/mL.However,seed extract had the strongest potential activity against Aspergillus niger and Candida albicans with minimum inhibitory concentration value of 500 μg/mL compared to pulp extract.Conclusions:Our results therefore demonstrated that ethanol and methanol extractions were more efficient in extracting antioxidants and bioactive compound in pulp and seed.These results support that these plant extracts can be used for the treatment of bacterial infections.
文摘A new guanidine derivative,complanatin has been isolated from the seeds of Chinese traditional medicine Astragalus complanatus R.Br..Its structure was established on the basis of spectral data and X-ray crystallographic analysis to be N-[3-carboxypropyl]-N-[3-methyl-2-butenyl]guanidine.
基金Supported by Natural Science Foundation in Chongqing(CSTC,2006BB1336)Applied Basic Research Program of Chongqing Education Department(KJ060308)
文摘[ Objective] The paper aimed to study the optimum extraction technology of pigments from petals of Matthiola incana（ L. ） R. Br. of differ- ent colors. [Method] Petal pigments of Aida variety （white violet）, Francesco variety （ red violet） and Arabella variety （ purple violet） were ana- lyzed by colorimetric reaction method and ultraviolet-visible spectrum method, and the optimum extraction technology of flavonoid compounds from petals of them was determined by single factor experiment and orthogonal experiment. [ Result] The optimum extraction conditions of white violet were as follows： with 95% ethanol ＋ 5% HCI as solvent, solid-liquid ratio （ 1 ： 40）, extraction time （3 h）, extraction temperature （65 ℃ ）. The opti- mum extraction conditions of red violet and purple violet were as follows： with acetone as solvent, solid-liquid ratio （1：40）, extraction time （3 h）, extraction temperature （65 ℃）. [ Conclusion] The study provided theoretical foundation for further development and exploitation of pigments from M. incana（ L. ） R. Br.
文摘为明确影响鹅绒藤分布的主导环境因子及其生态阈值,量化不同时期其潜在适生区,借助MaxEnt模型与ArcGIS空间分析技术,融合鹅绒藤在我国的301个分布点位信息及生物气候数据,对其在2个过去时期[末次盛冰期(约22000 a前)和全新世中期(约6000 a前)]、当前时期(1970—2000年)以及3个未来时期[2030s(2021—2040年)、2050s(2041—2060年)、2070s(2061—2080年)]的潜在适生区进行预测分析。结果表明,MaxEnt模型各时期受试者工作特征曲线下面积(Area under the curve,AUC)值均高于0.9,预测结果可靠。鹅绒藤的分布主要受年平均温度、温度季节性变异系数、降水量季节性变异系数和最湿月份降水量影响。当前时期下,鹅绒藤的总适生区面积达193.37675×10^(4)km^(2),其中高适生区面积为69.01563×10^(4)km^(2)。与当前时期相对比,鹅绒藤在末次盛冰期和全新世中期适生区扩张幅度较为显著。3个未来时期中,鹅绒藤在SSP370(区域竞争路径)气候变化情景下2070s适生区面积最大,在SSP126(可持续路径)气候变化情景下2030s适生区面积最小,但均比当前时期适生区面积有所扩张。综上,鹅绒藤在2个过去时期和3个未来时期不同气候情景下均呈现扩张趋势,反映了气候变化,尤其是碳循环所引起的温度变化对物种分布的阶段性影响。
