Quorum sensing is a chemical communication process that bacteria use to regulate collective behaviors. In Gram-positive bacteria, oligopeptides (called autoinducers) are the signaling molecules to elicit quorum sensin...Quorum sensing is a chemical communication process that bacteria use to regulate collective behaviors. In Gram-positive bacteria, oligopeptides (called autoinducers) are the signaling molecules to elicit quorum sensing. In Bacillus thuringiensis, NprR is a transcriptional regulator whose activity depends on the NprX signalling peptide. Bacillus thuringiensis is closely related to Bacillus cereus and Bacillus anthracis. The principal difference between them is that Bacillus thuringiensis is the only one that produced Cry protein. The aim of this study is to explore the relation of nprR and 16S rRNA genes in Bacillus thurin-giensis. Phylogenetic trees of nucleotide sequences of nprR and 16S rRNA genes were built. Sequences of fourteen new isolates from Papaloapan region were included in those phylo-genetic trees. In order to identify the isolates, a simple and fast methodology considering the Cry protein formation was used. The 16S rRNA phylogenetic tree allows identify eight isolates as Bacillus thuringiensis and the others as Bacillus spp. The nprR phylogenetic tree does not match with the 16S rRNA phylogenetic tree. This confirms that nprR is not a molecular marker for evolution. Most of the new isolates have the same NprR sequence (WTSDIVG). However, the SKPDIVG is the most common NprR sequence in thuringiensis species.展开更多
Piericidin A1, 3’-rhamnopiericidin A1, and a novel compound piericidin E, a new quorum-sensing (QS) inhibitor against Chromobacterium violaceum CV026, were isolated from the culture broth of Streptomyces sp. QS is we...Piericidin A1, 3’-rhamnopiericidin A1, and a novel compound piericidin E, a new quorum-sensing (QS) inhibitor against Chromobacterium violaceum CV026, were isolated from the culture broth of Streptomyces sp. QS is well known as a microbial signaling system and controls certain types of gene expression resulting in bioluminescence, biofilm formation, swarming motility, antibiotic biosynthesis, and virulence factor production. C. violaceum CV026 is commonly used to determine qualitative and quantitative QS activity. The structures of piericidin derivatives were characterized, and their QS activities were determined.展开更多
Although the aquaculture industry has undergone monumental development worldwide,the ever-present threats of infectious diseases have become a constraining factor,imperiling its sustainability.Antimicrobial resistance...Although the aquaculture industry has undergone monumental development worldwide,the ever-present threats of infectious diseases have become a constraining factor,imperiling its sustainability.Antimicrobial resistance(AMR)remains a real menace to industrial aquaculture due to the careless adoption of preventive therapies(antimicrobial therapeutic drugs)to forestall disease outbreaks in aquatic food production.Suitable strategies,or at least supplementary measures,should therefore be developed to curb the emergence and widespread transmission of AMR.Vaccination represents one of the primary options to substantially mitigate the economic damages imposed by emerging infectious diseases on global aquaculture;nevertheless,the availability of commercial aquatic vaccines is usually limited,and many vaccines only confer minimal or poor protection against infections(during the early stages of animal development).Accordingly,a large body of research has been enthusiastically exploring alternate approaches for managing animal health challenges.These efforts have led to the establishment of various biocontrol strategies,such as the versatile use of high-value functional ingredients(e.g.,probiotics,prebiotics,synbiotics,paraprobiotics,postbiotics,and phytogenics),phage therapy,and quorum-sensing interference(QSI),to promote the health and welfare of farmed aquatic species in a responsive or preventative manner.This review article addresses the state-of-the-art pertinent to biological control as an eco-friendly green approach for aquatic disease management,paving the route to a sustainable blue revolution.The potential biological mechanisms of these strategies are also described,along with the impediments to scientific progress and topics that merit further investigation.展开更多
Antibiotic-resistant bacterial pathogens pose substantial biosafety and health hazards,leading to millions of deaths each year.The evolution of bacterial virulence factors is mainly propelled by horizontal gene transf...Antibiotic-resistant bacterial pathogens pose substantial biosafety and health hazards,leading to millions of deaths each year.The evolution of bacterial virulence factors is mainly propelled by horizontal gene transfer(HGT).In addition to traditional antibiotics,antimicrobial strategies targeting biofilm-related virulence factors and quorum sensing(QS)-related virulence factors can effectively restrain drug-resistant bacteria.Future antivirulence strategies,encompassing natural drugs,antibiotic resistance inhibitors,monoclonal antibodies(mAbs),and vaccines,are in the development pipeline.Consequently,by disrupting virulence factors,these drugs can eliminate the ability of bacterial pathogens to cause disease.In conclusion,this Perspective comprehensively summarizes current anti-bacterial virulence factor strategies and prospects for future cutting-edge approaches,which may address the issues of antibacterial resistance and curtail the spread of pathogens in the future.展开更多
文摘Quorum sensing is a chemical communication process that bacteria use to regulate collective behaviors. In Gram-positive bacteria, oligopeptides (called autoinducers) are the signaling molecules to elicit quorum sensing. In Bacillus thuringiensis, NprR is a transcriptional regulator whose activity depends on the NprX signalling peptide. Bacillus thuringiensis is closely related to Bacillus cereus and Bacillus anthracis. The principal difference between them is that Bacillus thuringiensis is the only one that produced Cry protein. The aim of this study is to explore the relation of nprR and 16S rRNA genes in Bacillus thurin-giensis. Phylogenetic trees of nucleotide sequences of nprR and 16S rRNA genes were built. Sequences of fourteen new isolates from Papaloapan region were included in those phylo-genetic trees. In order to identify the isolates, a simple and fast methodology considering the Cry protein formation was used. The 16S rRNA phylogenetic tree allows identify eight isolates as Bacillus thuringiensis and the others as Bacillus spp. The nprR phylogenetic tree does not match with the 16S rRNA phylogenetic tree. This confirms that nprR is not a molecular marker for evolution. Most of the new isolates have the same NprR sequence (WTSDIVG). However, the SKPDIVG is the most common NprR sequence in thuringiensis species.
文摘Piericidin A1, 3’-rhamnopiericidin A1, and a novel compound piericidin E, a new quorum-sensing (QS) inhibitor against Chromobacterium violaceum CV026, were isolated from the culture broth of Streptomyces sp. QS is well known as a microbial signaling system and controls certain types of gene expression resulting in bioluminescence, biofilm formation, swarming motility, antibiotic biosynthesis, and virulence factor production. C. violaceum CV026 is commonly used to determine qualitative and quantitative QS activity. The structures of piericidin derivatives were characterized, and their QS activities were determined.
基金supported by research grants from the Ministry of Higher Education(MOHE),Malaysia,through the Higher Institution Centre of Excellence(HICoE)scheme(Grant Code:6369100)the Fundamental Research Grant Scheme(FRGS)(Grant Code:5540469)The lead author also gratefully acknowledges the support provided by the Universiti Putra Malaysia(UPM)Postdoctoral Research Fellowship Program.
文摘Although the aquaculture industry has undergone monumental development worldwide,the ever-present threats of infectious diseases have become a constraining factor,imperiling its sustainability.Antimicrobial resistance(AMR)remains a real menace to industrial aquaculture due to the careless adoption of preventive therapies(antimicrobial therapeutic drugs)to forestall disease outbreaks in aquatic food production.Suitable strategies,or at least supplementary measures,should therefore be developed to curb the emergence and widespread transmission of AMR.Vaccination represents one of the primary options to substantially mitigate the economic damages imposed by emerging infectious diseases on global aquaculture;nevertheless,the availability of commercial aquatic vaccines is usually limited,and many vaccines only confer minimal or poor protection against infections(during the early stages of animal development).Accordingly,a large body of research has been enthusiastically exploring alternate approaches for managing animal health challenges.These efforts have led to the establishment of various biocontrol strategies,such as the versatile use of high-value functional ingredients(e.g.,probiotics,prebiotics,synbiotics,paraprobiotics,postbiotics,and phytogenics),phage therapy,and quorum-sensing interference(QSI),to promote the health and welfare of farmed aquatic species in a responsive or preventative manner.This review article addresses the state-of-the-art pertinent to biological control as an eco-friendly green approach for aquatic disease management,paving the route to a sustainable blue revolution.The potential biological mechanisms of these strategies are also described,along with the impediments to scientific progress and topics that merit further investigation.
基金supported by the Science Technology and Innovation Commission of Shenzhen(JCYJ20210324115607021)the National Natural Science Foundation of China(32300068,32270196,91951204,32200155,32200053,and 32300060).
文摘Antibiotic-resistant bacterial pathogens pose substantial biosafety and health hazards,leading to millions of deaths each year.The evolution of bacterial virulence factors is mainly propelled by horizontal gene transfer(HGT).In addition to traditional antibiotics,antimicrobial strategies targeting biofilm-related virulence factors and quorum sensing(QS)-related virulence factors can effectively restrain drug-resistant bacteria.Future antivirulence strategies,encompassing natural drugs,antibiotic resistance inhibitors,monoclonal antibodies(mAbs),and vaccines,are in the development pipeline.Consequently,by disrupting virulence factors,these drugs can eliminate the ability of bacterial pathogens to cause disease.In conclusion,this Perspective comprehensively summarizes current anti-bacterial virulence factor strategies and prospects for future cutting-edge approaches,which may address the issues of antibacterial resistance and curtail the spread of pathogens in the future.
