Quinoa is pseudo cereal and considered as full nutritional food for its functional ingredients such as peptides,polysaccharides,saponins and polyphenols.Up to now,over 20 phenolic compounds have been reported in quino...Quinoa is pseudo cereal and considered as full nutritional food for its functional ingredients such as peptides,polysaccharides,saponins and polyphenols.Up to now,over 20 phenolic compounds have been reported in quinoa,and these polyphenols are related to anti-cancer,anti-inflammatory,anti-obesity,anti-diabetic and cardioprotective activities.Recently,more and more attentions are focused on quinoa in the food and pharmaceutical fields.Many new products such as bakery,beverage and meat product made from quinoa are popular in the market.This article presents a review of the literature on the function and application of polyphenols in quinoa.The review will benefit the researchers working with nutrition,functional diets of quinoa.展开更多
Soil salinity is a wo rldwide issue and a major threat to global food security.Salinity tolerance is a complex mechanism that is conferred by numerous molecular,physiological,and biochemical traits.Of critical importa...Soil salinity is a wo rldwide issue and a major threat to global food security.Salinity tolerance is a complex mechanism that is conferred by numerous molecular,physiological,and biochemical traits.Of critical importance are plant's ability to regulate redox balance without compromising reactive oxygen species(ROS)signalling and maintain cytosolic ion homeostasis.In this study,the mechanistic basis of H^(+) retention ability in leaf mesophyll(an important but highly sensitive plant tissue)was compared between halophytic quinoa and glycophytic spinach.Phenotypic data showed quinoa outperformed spinach under 100 to 500 mmol L^(-1) NaCl salinity.The major difference behind this differential salinity sensitivity was a differential H^(+) uptake in leaf mesophyll.Electrophysiological and molecular experiments revealed that a superior ability of mesophyll H^(+) retention in quinoa was conferred by three complementary mechanisms:(ⅰ)an intrinsically lower H^(+)-ATPase activity in quinoa(potentially as an energy saving strategy);(ⅱ)reduced sensitivity of H^(+) transporters to ROS;and(ⅲ)increased sensitivity of ROS-inducible Ca^(2+)-permeable channels,Moreover,the sensitivity of H^(+)-transport systems to ROS was further examined in NaCl-acclimated quinoa and spinach plants.The key factors differentiating between H^(+) retention in acclimated leaf mesophyll was associated with the reduced sensitivity and gene expression of H^(+)-permeable outward rectifying channel(GORK),Arabidopsis potassium transporter 1(AKT1),and high affinity potassium transporter 5(HAK5)to additional NaCl and ROS stress,along with the upregulation of ROS scavenging system.Taken together,our results showed that the tissue-specific and ROS-specific regulation of H^(+) retention are important for conferring salinity tolerant at least in the halophyte quinoa.展开更多
Quinoa(Chenopodium quinoa Willd.)is regarded as a superfood due to its exceptionally high nutritional value and ability to withstand stress.Six quinoa genotypes(viz.,SAU Quinoa-1,Regalona,GPBQ-1,GPBQ-2,GPBQ-3,and GPBQ...Quinoa(Chenopodium quinoa Willd.)is regarded as a superfood due to its exceptionally high nutritional value and ability to withstand stress.Six quinoa genotypes(viz.,SAU Quinoa-1,Regalona,GPBQ-1,GPBQ-2,GPBQ-3,and GPBQ-4)were characterized for morphological,yield,and nutritional quality attributes while being grown under the agro-climatic conditions of Bangladesh.The field experiment was carried out in the winter season(November 2022–March 2023)at the Agronomy Field Laboratory of the Department of Agronomy,Bangladesh Agricultural University,Mymensingh,using a randomized complete block design with three replications.Data on various qualitative and quantitative traits related to growth and yield-attributes were recorded.A large variability was observed among the genotypes for growth habit,stem color,panicle shape,and panicle color at maturity.The whitish color of the seed was found in GPBQ-3 which indicates less saponin content as confirmed by saponin test.Yield attributing quantitative traits(viz.,days to first flowering,days to maturity,plant height,stem diameter,panicle weight,1000-seed weight,yield plant^(-1),above-ground biomass,and harvest index)also showed significant variation among the genotypes studied.Genotypes SAU Quinoa-1 and GPBQ-2 were identified as early maturing genotypes.The higher yield plant^(-1) was recorded in GPBQ-1 and GPBQ-3 genotypes.The phenotypic coefficient of variation for the majority of the traits evaluated was slightly higher than the corresponding genotypic coefficient of variation.For stem diameter,panicle weight,1000-seed weight,yields plant^(-1),and above-ground biomass,high heritability and high genetic advancement were seen as percentages of the mean.Yield plant^(-1) showed significant positive correlation with days to first flowering,days to maturity,plant height,stem diameter,panicle weight,and above-ground biomass(0.568*,0.812***,0.744***,0.895***,0.993***,and 0.985***,respectively).The first two components accounted for 85.5%of the overall variation among the genotypes,according to principal component analysis.Significant variability was also found for seed mineral contents(viz.,calcium,copper,iron,potassium,magnesium,manganese,and zinc)in the studied genotypes.The SAU Quinoa-1 genotype contained the highest amount of calcium and zinc,whereas,the highest amount of potassium was recorded in the GPBQ-1 genotype.We found a non-significant variability in carbohydrate,protein,fat,fiber,and vitamins(viz.,thiamine,riboflavin,niacin,and folic acid)contents in the studied genotypes.Considering all the yield and nutritional quality traits under study,the genotypes GPBQ-1,GPBQ-3,and GPBQ-4 were selected for future variety development program.展开更多
Quinoa research aims to deeply understand its nutritional value,develop planting techniques,and explore food applications to promote quinoa industry development and improve human health.Future research directions incl...Quinoa research aims to deeply understand its nutritional value,develop planting techniques,and explore food applications to promote quinoa industry development and improve human health.Future research directions include further exploring nutritional functions,adaptive breeding,cultivation techniques and food processing of quinoa,so as to promote innovation and development in the quinoa industry.Expected outcomes include increased production,improved quality,expanded markets,diversified food sources,reduced environmental impact,and biodiversity protection.There are still challenges such as fluctuating market demand,resource constraints,insufficient nutritional knowledge,and fierce competition.Solutions may include education and publicity,diversified product lines,health certification and brand building,partnerships,new variety cultivation and sustainable farming,and resource sharing.Future research and practice will further promote innovation and development in the quinoa industry,making it one of the most important food and functional ingredients globally.展开更多
Pork sausages contain significantly high amounts of saturated fat and they have been linked to various cardiovascular diseases. Fat substitutes used to reduce the amount of saturated fat include starch, gums, soy, pla...Pork sausages contain significantly high amounts of saturated fat and they have been linked to various cardiovascular diseases. Fat substitutes used to reduce the amount of saturated fat include starch, gums, soy, plant oils, cereal-based substitutes like chia and oats. Due to modification, such sausages have been reported to have undesirable sensory and physicochemical properties, such as hardening and lowered emulsion stability. Quinoa is a nutritious pseudo cereal comprising all the essential amino acids and has been shown to have good binding abilities. However, its potential has not yet been fully utilized in product development, especially in the meat industry. This research study aimed to investigate the effect of quinoa flour and gum Arabic on the physicochemical, nutritional, and textural properties of pork sausages. Quinoa flour was used in sausage formulation to partially substitute pork fat, while gum Arabic from Acacia senegal var. kerensis was used to stabilize the emulsion. The resulting sausages were analyzed for crude protein, crude fat, moisture, expressible moisture, pH, in vitro protein digestibility, and texture. The results indicated that, increase in the levels of quinoa flour significantly (p < 0.05) increased the crude protein content from 11.83% to 17.94% and reduced the amount of crude fat from 29.73% to 10.41%. However, addition of quinoa led to a significant increase in hardness, gumminess and chewiness. On the other hand, increasing the levels of gum Arabic led to a significant (p < 0.05) decrease in cooking loss, expressible moisture and hardness but it increased ash content. Combining quinoa flour and gum Arabic in low fat pork sausages improved their crude protein, crude fat, ash content, hardness and gumminess properties. The results of this study showed that quinoa flour and gum Arabic can be utilized to produce nutritious and acceptable low-fat pork sausages.展开更多
Quinoa(Chenopodium quinoa)has recently gained popularity as a pseudo-cereal cultivated in various countries due to the nutritional and antioxidant benefits of its seeds,and its capacity to persist in water-stressed en...Quinoa(Chenopodium quinoa)has recently gained popularity as a pseudo-cereal cultivated in various countries due to the nutritional and antioxidant benefits of its seeds,and its capacity to persist in water-stressed environments.Our study aimed to assess the effects of native arbuscular mycorrhizal fungi(AMF)and local organic amendments on the metabolic responses and antioxidant activity of quinoa seeds under water-stressed conditions.To this end,quinoa plants were grown in soils inoculated with an indigenous mycorrhizal consortium AMF and amended with two types of compost from horse manure(HM)and green waste(GW)under two water regimes:well-watered(WW)75%and 25%field capacity(FC).The primary metabolite contents(sugars and total protein)of quinoa seeds were measured.Additionally,the study involved identifying and quantifying secondary metabolites,particularly phenolic compounds(quercetin,vanillic acid,rutin,coumaric acid,kaempferol,and tetraterpenoids carotenoids)in quinoa seeds were determined using high-performance liquid chromatography(HPLC).The individual application of AMF and HM increased the total protein content in quinoa seeds by 8%and 6%,respectively,in contrast to the water-stressed control plants(WS).Conversely,the combined application of AMF,GW,and HM led to a 21%increase in sugar content compared to the control seeds under water-stressed conditions.Additionally,HPLC analysis identified five phenolic compounds,namely quercetin,kaempferol,vanillic acid,coumaric acid,and rutin.Under WS conditions,the application of biostimulants,whether used individually or in combination,brought about a rise in the identified phenolic compounds,except rutin,compared to controls.Consequently,these findings suggest that using AMF,either alone or in combination with HM and/or GW,can enhance the total protein content of quinoa seeds,help sustain higher levels of both primary and secondary metabolites under water stress conditions,thereby enhancing tolerance and reducing the detrimental impact of water stress on quinoa plants.展开更多
Drought stress negatively impacts agricultural crop yields.By using mineral fertilizers and chemical regulators to encourage plant development and growth,its impact can be mitigated.The current study revealed that exo...Drought stress negatively impacts agricultural crop yields.By using mineral fertilizers and chemical regulators to encourage plant development and growth,its impact can be mitigated.The current study revealed that exogenous silicon(Si)(potassium silicate;K2Si2O5 at 1000 ppm)and molybdenum(Mo)(ammonium molybdate;(NH4)6Mo7O24•4H2O at 100 ppm)improved drought tolerance in quinoa(Chenopodium quinoa Willd).The research was conducted in a randomized complete block design with three biological replicates.The treatments comprised T0(control,water spray),T4(drought stress),and T1,T2,T3,T5,T6,and T7,i.e.,foliar applications of silicon and molybdenum solutions individually and in combination.Results revealed that drought stress predominantly affected the quinoa yield by decreasing the growth,physiological,biochemical,metabolic,hormonal,antioxidant,and ionic attributes.On the contrary,the supplementation of Si and Mo enhanced the growth attributes(shoot,panicle,and root length,No.of leaves per plant,shoot and panicle fresh/dry weight,root fresh/dry weight,No.of seeds and seeds fresh weight per plant),physiological traits(relative water content,chlorophyll,and carotenoids content),biochemical characteristics(total soluble sugars,protein and lipid content),metabolic attributes(total phenolic,flavonoids,tannins,lycopene,carotene),hormonal contents(indoleacetic acid(IAA),gibberellic acid(GA),salicylic acid(SA)),enzymatic and non-enzymatic antioxidants(catalase,peroxidase and ascorbic acid),and ionic content(potassium(K),(calcium)Ca,(magnesium)Mg,Si and Mo).Under drought stress,Si and Mo reduced electrolyte leakage,abscisic acid(ABA)content,H_(2)O_(2) production,and sodium uptake.In addition,combined Si and Mo supplementation elevated the expression of the sucrose non-fermenting 1(SNF1)-associated protein kinase 2(SnRK2)(CqSNRK2.10)gene in quinoa under drought stress vs.control,signifying an essential regulatory function for Si and Mo-induced drought stress tolerance.These results imply that the exogenous administration of Si and Mo in combination might be an efficient method to alleviate drought stress on quinoa.展开更多
Quinoa is a good source of phenolics,which both exist as free and bound forms.In order to mark clear the characteristic free and bound phenolics in different quinoa samples,in this study,characteristic free and bound ...Quinoa is a good source of phenolics,which both exist as free and bound forms.In order to mark clear the characteristic free and bound phenolics in different quinoa samples,in this study,characteristic free and bound phenolics in three colored quinoas including WQ(white quinoa),RQ(red quinoa)and BQ(black quinoa)were investigated.Result showed a total of 14 phenolics both acted as free and bound form were analyzed in three colored quinoas(WQ,RQ and BQ).Gallic acid,vanillic acid,epicatechin,p-coumaric acid and quercetin existed both as free and bound forms were common phenolics in quinoas.The highest total free phenolics(238.10 mg/kg)and bound phenolics(3377.75 mg/kg)were presented in WQ and RQ,respectively.It indicated WQ and RQ were respectively good source of free and bound phenolics.Moreover,characteristic free and bound phenolics in three colored quinoas could be well analyzed by principal component analysis(PCA),indicating it was an effective and reliable method in distinguishing three colored quinoas based on their characteristic free and bound phenolics,respectively.展开更多
To rapidly obtain high-quality genomic DNA from Chenopodium quinoa Willd, the genomic DAN in different tissues （leaves, stems and roots） of Chenopodi- um quinoa Willd was extracted by modified CTAB method, SDS metho...To rapidly obtain high-quality genomic DNA from Chenopodium quinoa Willd, the genomic DAN in different tissues （leaves, stems and roots） of Chenopodi- um quinoa Willd was extracted by modified CTAB method, SDS method and high- salt Iow-pH method, respectively. The quality and yield of extracted DNA was deter- mined using agarose gel electrophoresis and UV spectrophotometry. At the same time, the PCR-SSR and SSCP molecular detection was also performed. The results showed that the gel test strips, without obvious decomposition, of all the extraction methods were relatively obvious; the genomic DNA yield extracted by modified CTAB method was highest, followed by that by SDS method, and the genomic DNA extracted by high-salt Iow-pH method was lowest： the genomic DNA yields extracted by different methods from Chenopodium quinoa Wiltd leaves were all high- er than those from roots and stems; the quality of Chenopodium quinoa Willd ge- nomic DNA extracted by modified CTAB method and high-salt Iow-pH method was better, and polyphenols, polysaccharides and other impurities were removed more completely. The PCR-SSR and SSCP detection results showed that the genomic DNA extracted by different methods from different tissues of Chenopodium quinoa Willd all could be better amplified, and high-quality strips could be obtained. So the Chenopodium quinoa Willd genomic DNA extracted by the three methods all can be used for subsequent molecular biology research.展开更多
[Objective] To explore a rapid determination method for fiber content in grains of quinoa. [Method] Near infrared spectra of 100 quinoa samples were collected. The predicted models for quantitative analysis of fiber c...[Objective] To explore a rapid determination method for fiber content in grains of quinoa. [Method] Near infrared spectra of 100 quinoa samples were collected. The predicted models for quantitative analysis of fiber contents in the grains were built using near infrared transmittance spectroscopy （NITS）. [Result] In the wavelength range of 10 000-4 000 cm-1, the near infrared quantitative model of quinoa crude fiber was set up via first derivative ＋ vector normalization preprocessing and combining with the data from chemical methods. The calibration and prediction effect were best, and then the cross validation determination coefficient （FFcv） and external validation determination coefficient （FFval） of fiber by near in- frared quantitative model were 0.884 8 and 0.876 1, respectively. [Conclusion] the model of NITS about complete grains quinoa fiber can be available for fast detecting quinoa fiber content.展开更多
Callus induction effects of nine varieties of Chenopodium quinoa Willd. were compared by taking stem segments and cotyledons of C. quinoa as the ex- plants. At the same time, callus JnductJon of stem segments was opti...Callus induction effects of nine varieties of Chenopodium quinoa Willd. were compared by taking stem segments and cotyledons of C. quinoa as the ex- plants. At the same time, callus JnductJon of stem segments was optimized, as well as the callus proliferation system. Research results showed that the optimal explant for callus induction was stem segment. The average callus induction rate of nine varieties reached 90% in culture medium MS ＋ 0.5 mg/L 2, 4-D. In the callus opti- mization test, treatment VI （MS ＋ 0.5 mg/L 2, 4-D ＋ 0.5 mg/L KT ＋ 0.5 mg/L NAA） and treatment II （MS ＋ 0.5 mg/L 2, 4-D） had close induction rate, but the callus morphology was greatly different. The latter had loose, glossy and yellowish white calluses. Therefore, culture medium MS ＋ 0.5 mg/L 2, 4-D was the optimal for callus induction. And using 2, 4-D together with KT and NAA could significantly increase the proliferation rate of calluses.展开更多
Quinoa (Chenopodium quinoa Willd.) is a halophytic, allotetraploid grain crop of the Amaranthaceae family with impressive drought tolerance, nutritional content and an increasing worldwide market. Here we report the r...Quinoa (Chenopodium quinoa Willd.) is a halophytic, allotetraploid grain crop of the Amaranthaceae family with impressive drought tolerance, nutritional content and an increasing worldwide market. Here we report the results of an RNA-seq transcriptome analysis of Chenopodium quinoa using four water treatments (field capacity to drought) on the varieties “Ingapirca” (representing valley ecotypes) and “Ollague” (representing Altiplano Salares ecotypes). Physiological results, including growth rate, photosynthetic rate, stomatal conductance, and stem water potential, support the earlier findings that the Altiplano Salares ecotypes display greater tolerance to drought-like stress conditions than the valley ecotypes. cDNA libraries from root tissue samples for each variety × treatment combination were sequenced using Illumina Hi-Seq technology in an RNA-seq experiment. De novo assembly of the transcriptome generated 20,337 unique transcripts. Gene expression analysis of the RNA-seq data identified 462 putative gene products that showed differential expression based on treatment, and 27 putative gene products differentially expressed based on variety × treatment, including significant expression differences in root tissue in response to increasing water stress. BLAST searches and gene ontology analysis show an overlap between drought tolerance stress and other abiotic stress mechanisms.展开更多
Seeds with colored testa (seed coat) contain high concentrations of polyphenolic compounds that exhibit high levels of antioxidant activity. Common processing procedures, such as cooking and baking, decrease the level...Seeds with colored testa (seed coat) contain high concentrations of polyphenolic compounds that exhibit high levels of antioxidant activity. Common processing procedures, such as cooking and baking, decrease the levels of these bioactive compounds and consequently, overall antioxidant activity. Here, the effects of baking and cooking processes were examined on total phenolic content (TPC), total flavonoid content (TFC) and ferric-reducing ability of plasma antioxidant activity (FRAP AA) of red and yellow quinoa seeds. Our results indicate that red quinoa seed contains significantly higher levels of TPC, TFC and FRAP AA than yellow quinoa seeds. In addition, cooked and baked quinoa seeds retain most of their TPC, TFC and FRAP AA in the final product. Thus, red quinoa seeds processed by these two methods might be considered a functional food, in addition to its traditional role of providing dietary proteins. Due to their high antioxidant activity, red quinoa seeds might also contribute significantly to the management and/or prevention of degenerative diseases associated with free radical damage.展开更多
Quinoa (Chenopodium quinoa Willd.) is a staple food produced mainly by small-scale subsistence farmers in Peru’s highland. Dry seeds (cv. Pasankalla) were irradiated with doses of 150 Gy, 250 Gy and 350 Gy. In the M1...Quinoa (Chenopodium quinoa Willd.) is a staple food produced mainly by small-scale subsistence farmers in Peru’s highland. Dry seeds (cv. Pasankalla) were irradiated with doses of 150 Gy, 250 Gy and 350 Gy. In the M1 generation, the germination process was delayed with increasing radiation dose;seedling height, root length and leaf development were most reduced at 250 Gy and at 350 Gy, no plants survived. In M2, the maximum spectrum of chlorophyll mutations corresponded to 150 Gy and the maximum frequency to 250 Gy. The chlorine mutation was predominant, followed by xantha. Changes were registered for branch number, pedicel length, plant height, life-cycle duration, stem and foliage colour, and leaf morphology at the two doses, with improvements in plant type. More than one mutation per plant was found, especially at 250 Gy. In M3, the same spectrum of mutations was observed, along with a valuable change in grain colour.展开更多
Processing bioactive peptides from natural sources using electrodialysis with ultrafiltration membranes(EDUF)have gained attention since it can fractionate in terms of their charge and molecular weight.Quinoa is a pse...Processing bioactive peptides from natural sources using electrodialysis with ultrafiltration membranes(EDUF)have gained attention since it can fractionate in terms of their charge and molecular weight.Quinoa is a pseudo-cereal highlighted by its high protein content,amino acid profile and adapting growing conditions.The present work aimed at the production of quinoa peptides through fractionation using EDUF and to test the fractions according to antihypertensive and antidiabetic activity.Experimental data showed the production of peptides ranging between 0.4 and 1.5 k Da.Cationic(CQPF)(3.01%),anionic(AQPF)(1.18%)and the electrically neutral fraction quinoa protein hydrolysate(QPH)-EDUF(~95%)were obtained.In-vitro studies showed the highest glucose uptake modulation in L6 cell skeletal myoblasts in presence of QPH-EDUF and AQPF(17%and 11%)indicating potential antidiabetic activity.The antihypertensive effect studied in-vivo in spontaneously hypertensive rats(SHR),showed a decrease in systolic blood pressure in presence of the fractionated peptides,being 100 mg/kg a dose comparable to Captopril(positive control).These results contribute to the current knowledge of bioactive peptides from quinoa by reporting the relevance of EDUF as tool to produce selected peptide fractions.Nevertheless,further characterization is needed towards peptide sequencing,their respective role in the metabolism and scaling-up production using EDUF.展开更多
文摘Quinoa is pseudo cereal and considered as full nutritional food for its functional ingredients such as peptides,polysaccharides,saponins and polyphenols.Up to now,over 20 phenolic compounds have been reported in quinoa,and these polyphenols are related to anti-cancer,anti-inflammatory,anti-obesity,anti-diabetic and cardioprotective activities.Recently,more and more attentions are focused on quinoa in the food and pharmaceutical fields.Many new products such as bakery,beverage and meat product made from quinoa are popular in the market.This article presents a review of the literature on the function and application of polyphenols in quinoa.The review will benefit the researchers working with nutrition,functional diets of quinoa.
基金supported by the Grassland Ecological Restoration and Management Project of General Grassland Station of Xinjiang (XJCYZZ202102)。
文摘Soil salinity is a wo rldwide issue and a major threat to global food security.Salinity tolerance is a complex mechanism that is conferred by numerous molecular,physiological,and biochemical traits.Of critical importance are plant's ability to regulate redox balance without compromising reactive oxygen species(ROS)signalling and maintain cytosolic ion homeostasis.In this study,the mechanistic basis of H^(+) retention ability in leaf mesophyll(an important but highly sensitive plant tissue)was compared between halophytic quinoa and glycophytic spinach.Phenotypic data showed quinoa outperformed spinach under 100 to 500 mmol L^(-1) NaCl salinity.The major difference behind this differential salinity sensitivity was a differential H^(+) uptake in leaf mesophyll.Electrophysiological and molecular experiments revealed that a superior ability of mesophyll H^(+) retention in quinoa was conferred by three complementary mechanisms:(ⅰ)an intrinsically lower H^(+)-ATPase activity in quinoa(potentially as an energy saving strategy);(ⅱ)reduced sensitivity of H^(+) transporters to ROS;and(ⅲ)increased sensitivity of ROS-inducible Ca^(2+)-permeable channels,Moreover,the sensitivity of H^(+)-transport systems to ROS was further examined in NaCl-acclimated quinoa and spinach plants.The key factors differentiating between H^(+) retention in acclimated leaf mesophyll was associated with the reduced sensitivity and gene expression of H^(+)-permeable outward rectifying channel(GORK),Arabidopsis potassium transporter 1(AKT1),and high affinity potassium transporter 5(HAK5)to additional NaCl and ROS stress,along with the upregulation of ROS scavenging system.Taken together,our results showed that the tissue-specific and ROS-specific regulation of H^(+) retention are important for conferring salinity tolerant at least in the halophyte quinoa.
文摘Quinoa(Chenopodium quinoa Willd.)is regarded as a superfood due to its exceptionally high nutritional value and ability to withstand stress.Six quinoa genotypes(viz.,SAU Quinoa-1,Regalona,GPBQ-1,GPBQ-2,GPBQ-3,and GPBQ-4)were characterized for morphological,yield,and nutritional quality attributes while being grown under the agro-climatic conditions of Bangladesh.The field experiment was carried out in the winter season(November 2022–March 2023)at the Agronomy Field Laboratory of the Department of Agronomy,Bangladesh Agricultural University,Mymensingh,using a randomized complete block design with three replications.Data on various qualitative and quantitative traits related to growth and yield-attributes were recorded.A large variability was observed among the genotypes for growth habit,stem color,panicle shape,and panicle color at maturity.The whitish color of the seed was found in GPBQ-3 which indicates less saponin content as confirmed by saponin test.Yield attributing quantitative traits(viz.,days to first flowering,days to maturity,plant height,stem diameter,panicle weight,1000-seed weight,yield plant^(-1),above-ground biomass,and harvest index)also showed significant variation among the genotypes studied.Genotypes SAU Quinoa-1 and GPBQ-2 were identified as early maturing genotypes.The higher yield plant^(-1) was recorded in GPBQ-1 and GPBQ-3 genotypes.The phenotypic coefficient of variation for the majority of the traits evaluated was slightly higher than the corresponding genotypic coefficient of variation.For stem diameter,panicle weight,1000-seed weight,yields plant^(-1),and above-ground biomass,high heritability and high genetic advancement were seen as percentages of the mean.Yield plant^(-1) showed significant positive correlation with days to first flowering,days to maturity,plant height,stem diameter,panicle weight,and above-ground biomass(0.568*,0.812***,0.744***,0.895***,0.993***,and 0.985***,respectively).The first two components accounted for 85.5%of the overall variation among the genotypes,according to principal component analysis.Significant variability was also found for seed mineral contents(viz.,calcium,copper,iron,potassium,magnesium,manganese,and zinc)in the studied genotypes.The SAU Quinoa-1 genotype contained the highest amount of calcium and zinc,whereas,the highest amount of potassium was recorded in the GPBQ-1 genotype.We found a non-significant variability in carbohydrate,protein,fat,fiber,and vitamins(viz.,thiamine,riboflavin,niacin,and folic acid)contents in the studied genotypes.Considering all the yield and nutritional quality traits under study,the genotypes GPBQ-1,GPBQ-3,and GPBQ-4 were selected for future variety development program.
基金Supported by Beijing Science and Technology Program(Z201100008020006).
文摘Quinoa research aims to deeply understand its nutritional value,develop planting techniques,and explore food applications to promote quinoa industry development and improve human health.Future research directions include further exploring nutritional functions,adaptive breeding,cultivation techniques and food processing of quinoa,so as to promote innovation and development in the quinoa industry.Expected outcomes include increased production,improved quality,expanded markets,diversified food sources,reduced environmental impact,and biodiversity protection.There are still challenges such as fluctuating market demand,resource constraints,insufficient nutritional knowledge,and fierce competition.Solutions may include education and publicity,diversified product lines,health certification and brand building,partnerships,new variety cultivation and sustainable farming,and resource sharing.Future research and practice will further promote innovation and development in the quinoa industry,making it one of the most important food and functional ingredients globally.
文摘Pork sausages contain significantly high amounts of saturated fat and they have been linked to various cardiovascular diseases. Fat substitutes used to reduce the amount of saturated fat include starch, gums, soy, plant oils, cereal-based substitutes like chia and oats. Due to modification, such sausages have been reported to have undesirable sensory and physicochemical properties, such as hardening and lowered emulsion stability. Quinoa is a nutritious pseudo cereal comprising all the essential amino acids and has been shown to have good binding abilities. However, its potential has not yet been fully utilized in product development, especially in the meat industry. This research study aimed to investigate the effect of quinoa flour and gum Arabic on the physicochemical, nutritional, and textural properties of pork sausages. Quinoa flour was used in sausage formulation to partially substitute pork fat, while gum Arabic from Acacia senegal var. kerensis was used to stabilize the emulsion. The resulting sausages were analyzed for crude protein, crude fat, moisture, expressible moisture, pH, in vitro protein digestibility, and texture. The results indicated that, increase in the levels of quinoa flour significantly (p < 0.05) increased the crude protein content from 11.83% to 17.94% and reduced the amount of crude fat from 29.73% to 10.41%. However, addition of quinoa led to a significant increase in hardness, gumminess and chewiness. On the other hand, increasing the levels of gum Arabic led to a significant (p < 0.05) decrease in cooking loss, expressible moisture and hardness but it increased ash content. Combining quinoa flour and gum Arabic in low fat pork sausages improved their crude protein, crude fat, ash content, hardness and gumminess properties. The results of this study showed that quinoa flour and gum Arabic can be utilized to produce nutritious and acceptable low-fat pork sausages.
基金funded by the Tuniso-Moroccan Mixed Laboratories(LMTM)of Plant Physiology and Biotechnology and Climate Change LPBV2C(LR11ES09)the FOSC Project(Sus-Agri-CC)from the European Union’s Horizon 2020 Research and Innovation Program under grant agreement No.862555.
文摘Quinoa(Chenopodium quinoa)has recently gained popularity as a pseudo-cereal cultivated in various countries due to the nutritional and antioxidant benefits of its seeds,and its capacity to persist in water-stressed environments.Our study aimed to assess the effects of native arbuscular mycorrhizal fungi(AMF)and local organic amendments on the metabolic responses and antioxidant activity of quinoa seeds under water-stressed conditions.To this end,quinoa plants were grown in soils inoculated with an indigenous mycorrhizal consortium AMF and amended with two types of compost from horse manure(HM)and green waste(GW)under two water regimes:well-watered(WW)75%and 25%field capacity(FC).The primary metabolite contents(sugars and total protein)of quinoa seeds were measured.Additionally,the study involved identifying and quantifying secondary metabolites,particularly phenolic compounds(quercetin,vanillic acid,rutin,coumaric acid,kaempferol,and tetraterpenoids carotenoids)in quinoa seeds were determined using high-performance liquid chromatography(HPLC).The individual application of AMF and HM increased the total protein content in quinoa seeds by 8%and 6%,respectively,in contrast to the water-stressed control plants(WS).Conversely,the combined application of AMF,GW,and HM led to a 21%increase in sugar content compared to the control seeds under water-stressed conditions.Additionally,HPLC analysis identified five phenolic compounds,namely quercetin,kaempferol,vanillic acid,coumaric acid,and rutin.Under WS conditions,the application of biostimulants,whether used individually or in combination,brought about a rise in the identified phenolic compounds,except rutin,compared to controls.Consequently,these findings suggest that using AMF,either alone or in combination with HM and/or GW,can enhance the total protein content of quinoa seeds,help sustain higher levels of both primary and secondary metabolites under water stress conditions,thereby enhancing tolerance and reducing the detrimental impact of water stress on quinoa plants.
基金the Researchers Supporting Project No.RSP2024R191,King Saud University,Riyadh,Saudi Arabiasupported by the IFS-International Foundation for Science,Stockholm,Sweden(C/5895-1).
文摘Drought stress negatively impacts agricultural crop yields.By using mineral fertilizers and chemical regulators to encourage plant development and growth,its impact can be mitigated.The current study revealed that exogenous silicon(Si)(potassium silicate;K2Si2O5 at 1000 ppm)and molybdenum(Mo)(ammonium molybdate;(NH4)6Mo7O24•4H2O at 100 ppm)improved drought tolerance in quinoa(Chenopodium quinoa Willd).The research was conducted in a randomized complete block design with three biological replicates.The treatments comprised T0(control,water spray),T4(drought stress),and T1,T2,T3,T5,T6,and T7,i.e.,foliar applications of silicon and molybdenum solutions individually and in combination.Results revealed that drought stress predominantly affected the quinoa yield by decreasing the growth,physiological,biochemical,metabolic,hormonal,antioxidant,and ionic attributes.On the contrary,the supplementation of Si and Mo enhanced the growth attributes(shoot,panicle,and root length,No.of leaves per plant,shoot and panicle fresh/dry weight,root fresh/dry weight,No.of seeds and seeds fresh weight per plant),physiological traits(relative water content,chlorophyll,and carotenoids content),biochemical characteristics(total soluble sugars,protein and lipid content),metabolic attributes(total phenolic,flavonoids,tannins,lycopene,carotene),hormonal contents(indoleacetic acid(IAA),gibberellic acid(GA),salicylic acid(SA)),enzymatic and non-enzymatic antioxidants(catalase,peroxidase and ascorbic acid),and ionic content(potassium(K),(calcium)Ca,(magnesium)Mg,Si and Mo).Under drought stress,Si and Mo reduced electrolyte leakage,abscisic acid(ABA)content,H_(2)O_(2) production,and sodium uptake.In addition,combined Si and Mo supplementation elevated the expression of the sucrose non-fermenting 1(SNF1)-associated protein kinase 2(SnRK2)(CqSNRK2.10)gene in quinoa under drought stress vs.control,signifying an essential regulatory function for Si and Mo-induced drought stress tolerance.These results imply that the exogenous administration of Si and Mo in combination might be an efficient method to alleviate drought stress on quinoa.
基金support of Youth Project of Liaoning Provincial Education Department(JYTQN2023334)Anhui Provincial Department of Education Key Research Project on Natural Science in Higher Education Institutions(KJ2021A1072)Science and Technology Plan Project of Chuzhou Science and Technology Bureau(2021ZD023).
文摘Quinoa is a good source of phenolics,which both exist as free and bound forms.In order to mark clear the characteristic free and bound phenolics in different quinoa samples,in this study,characteristic free and bound phenolics in three colored quinoas including WQ(white quinoa),RQ(red quinoa)and BQ(black quinoa)were investigated.Result showed a total of 14 phenolics both acted as free and bound form were analyzed in three colored quinoas(WQ,RQ and BQ).Gallic acid,vanillic acid,epicatechin,p-coumaric acid and quercetin existed both as free and bound forms were common phenolics in quinoas.The highest total free phenolics(238.10 mg/kg)and bound phenolics(3377.75 mg/kg)were presented in WQ and RQ,respectively.It indicated WQ and RQ were respectively good source of free and bound phenolics.Moreover,characteristic free and bound phenolics in three colored quinoas could be well analyzed by principal component analysis(PCA),indicating it was an effective and reliable method in distinguishing three colored quinoas based on their characteristic free and bound phenolics,respectively.
基金Supported by National Natural Science Foundation of China(31301372)Key Project of Science and Technology Plan of Zhejiang Province(2011C12030)Innovation Training Project of Zhejiang Agriculture and Forestry University(201301004)~~
文摘To rapidly obtain high-quality genomic DNA from Chenopodium quinoa Willd, the genomic DAN in different tissues （leaves, stems and roots） of Chenopodi- um quinoa Willd was extracted by modified CTAB method, SDS method and high- salt Iow-pH method, respectively. The quality and yield of extracted DNA was deter- mined using agarose gel electrophoresis and UV spectrophotometry. At the same time, the PCR-SSR and SSCP molecular detection was also performed. The results showed that the gel test strips, without obvious decomposition, of all the extraction methods were relatively obvious; the genomic DNA yield extracted by modified CTAB method was highest, followed by that by SDS method, and the genomic DNA extracted by high-salt Iow-pH method was lowest： the genomic DNA yields extracted by different methods from Chenopodium quinoa Wiltd leaves were all high- er than those from roots and stems; the quality of Chenopodium quinoa Willd ge- nomic DNA extracted by modified CTAB method and high-salt Iow-pH method was better, and polyphenols, polysaccharides and other impurities were removed more completely. The PCR-SSR and SSCP detection results showed that the genomic DNA extracted by different methods from different tissues of Chenopodium quinoa Willd all could be better amplified, and high-quality strips could be obtained. So the Chenopodium quinoa Willd genomic DNA extracted by the three methods all can be used for subsequent molecular biology research.
基金Supported by the Collection and Arrangement of Crop Germplasm Resources in Shanxi Province(2016zzcx-17)the Special Fund for the Protection and Utilization of Crop Germplasm Resources of the Ministry of Agriculture(2015NWB030-07)+1 种基金the Project of the National Science and Technology Infrastructure of the Ministry of Science and Technology and the Ministry of Finance(NICGR2015-026)the Special Fund for Seed Industry of Shanxi Province(2016zyzx41)~~
文摘[Objective] To explore a rapid determination method for fiber content in grains of quinoa. [Method] Near infrared spectra of 100 quinoa samples were collected. The predicted models for quantitative analysis of fiber contents in the grains were built using near infrared transmittance spectroscopy （NITS）. [Result] In the wavelength range of 10 000-4 000 cm-1, the near infrared quantitative model of quinoa crude fiber was set up via first derivative ＋ vector normalization preprocessing and combining with the data from chemical methods. The calibration and prediction effect were best, and then the cross validation determination coefficient （FFcv） and external validation determination coefficient （FFval） of fiber by near in- frared quantitative model were 0.884 8 and 0.876 1, respectively. [Conclusion] the model of NITS about complete grains quinoa fiber can be available for fast detecting quinoa fiber content.
基金Supported by National Natural Sciences Foundation of China(31301372)the Major Science and Technology Project of Zhejiang Province(2011C12030)the Haixi Prefecture science and technology project of Qinghai Province(2012-Y01)~~
文摘Callus induction effects of nine varieties of Chenopodium quinoa Willd. were compared by taking stem segments and cotyledons of C. quinoa as the ex- plants. At the same time, callus JnductJon of stem segments was optimized, as well as the callus proliferation system. Research results showed that the optimal explant for callus induction was stem segment. The average callus induction rate of nine varieties reached 90% in culture medium MS ＋ 0.5 mg/L 2, 4-D. In the callus opti- mization test, treatment VI （MS ＋ 0.5 mg/L 2, 4-D ＋ 0.5 mg/L KT ＋ 0.5 mg/L NAA） and treatment II （MS ＋ 0.5 mg/L 2, 4-D） had close induction rate, but the callus morphology was greatly different. The latter had loose, glossy and yellowish white calluses. Therefore, culture medium MS ＋ 0.5 mg/L 2, 4-D was the optimal for callus induction. And using 2, 4-D together with KT and NAA could significantly increase the proliferation rate of calluses.
文摘Quinoa (Chenopodium quinoa Willd.) is a halophytic, allotetraploid grain crop of the Amaranthaceae family with impressive drought tolerance, nutritional content and an increasing worldwide market. Here we report the results of an RNA-seq transcriptome analysis of Chenopodium quinoa using four water treatments (field capacity to drought) on the varieties “Ingapirca” (representing valley ecotypes) and “Ollague” (representing Altiplano Salares ecotypes). Physiological results, including growth rate, photosynthetic rate, stomatal conductance, and stem water potential, support the earlier findings that the Altiplano Salares ecotypes display greater tolerance to drought-like stress conditions than the valley ecotypes. cDNA libraries from root tissue samples for each variety × treatment combination were sequenced using Illumina Hi-Seq technology in an RNA-seq experiment. De novo assembly of the transcriptome generated 20,337 unique transcripts. Gene expression analysis of the RNA-seq data identified 462 putative gene products that showed differential expression based on treatment, and 27 putative gene products differentially expressed based on variety × treatment, including significant expression differences in root tissue in response to increasing water stress. BLAST searches and gene ontology analysis show an overlap between drought tolerance stress and other abiotic stress mechanisms.
文摘Seeds with colored testa (seed coat) contain high concentrations of polyphenolic compounds that exhibit high levels of antioxidant activity. Common processing procedures, such as cooking and baking, decrease the levels of these bioactive compounds and consequently, overall antioxidant activity. Here, the effects of baking and cooking processes were examined on total phenolic content (TPC), total flavonoid content (TFC) and ferric-reducing ability of plasma antioxidant activity (FRAP AA) of red and yellow quinoa seeds. Our results indicate that red quinoa seed contains significantly higher levels of TPC, TFC and FRAP AA than yellow quinoa seeds. In addition, cooked and baked quinoa seeds retain most of their TPC, TFC and FRAP AA in the final product. Thus, red quinoa seeds processed by these two methods might be considered a functional food, in addition to its traditional role of providing dietary proteins. Due to their high antioxidant activity, red quinoa seeds might also contribute significantly to the management and/or prevention of degenerative diseases associated with free radical damage.
文摘Quinoa (Chenopodium quinoa Willd.) is a staple food produced mainly by small-scale subsistence farmers in Peru’s highland. Dry seeds (cv. Pasankalla) were irradiated with doses of 150 Gy, 250 Gy and 350 Gy. In the M1 generation, the germination process was delayed with increasing radiation dose;seedling height, root length and leaf development were most reduced at 250 Gy and at 350 Gy, no plants survived. In M2, the maximum spectrum of chlorophyll mutations corresponded to 150 Gy and the maximum frequency to 250 Gy. The chlorine mutation was predominant, followed by xantha. Changes were registered for branch number, pedicel length, plant height, life-cycle duration, stem and foliage colour, and leaf morphology at the two doses, with improvements in plant type. More than one mutation per plant was found, especially at 250 Gy. In M3, the same spectrum of mutations was observed, along with a valuable change in grain colour.
基金financially supported by the Postdoctoral Fellowship N°3190683 of Dr.Adrián González-Munoz from the Chilean Agencia Nacional de Investigación y Desarrollo(ANID)the Natural Sciences and Engineering Research Council of Canada(NSERC)Discovery Grant Program(Grant SD RGPIN-2018-04128 of Prof.Laurent Bazinet)。
文摘Processing bioactive peptides from natural sources using electrodialysis with ultrafiltration membranes(EDUF)have gained attention since it can fractionate in terms of their charge and molecular weight.Quinoa is a pseudo-cereal highlighted by its high protein content,amino acid profile and adapting growing conditions.The present work aimed at the production of quinoa peptides through fractionation using EDUF and to test the fractions according to antihypertensive and antidiabetic activity.Experimental data showed the production of peptides ranging between 0.4 and 1.5 k Da.Cationic(CQPF)(3.01%),anionic(AQPF)(1.18%)and the electrically neutral fraction quinoa protein hydrolysate(QPH)-EDUF(~95%)were obtained.In-vitro studies showed the highest glucose uptake modulation in L6 cell skeletal myoblasts in presence of QPH-EDUF and AQPF(17%and 11%)indicating potential antidiabetic activity.The antihypertensive effect studied in-vivo in spontaneously hypertensive rats(SHR),showed a decrease in systolic blood pressure in presence of the fractionated peptides,being 100 mg/kg a dose comparable to Captopril(positive control).These results contribute to the current knowledge of bioactive peptides from quinoa by reporting the relevance of EDUF as tool to produce selected peptide fractions.Nevertheless,further characterization is needed towards peptide sequencing,their respective role in the metabolism and scaling-up production using EDUF.
