The fluorescence spectra of Quin 2, (2-[(2-bis-[carboxymethyl] amino-5-methylphenoxy) methyl]-6-methoxy-8-bis [carboxymethyl] aminoquiniline), a Ca2+ probe, were investigated upon incubation with Ca2+ or La3+. The res...The fluorescence spectra of Quin 2, (2-[(2-bis-[carboxymethyl] amino-5-methylphenoxy) methyl]-6-methoxy-8-bis [carboxymethyl] aminoquiniline), a Ca2+ probe, were investigated upon incubation with Ca2+ or La3+. The results showed that binding of La3+ to Quin 2 resulted in different fluorescent spectrum from that of Ca2+. Based on this observation, a fluorescent method was developed for simultaneously determination of the dissociation rates of Ca2+ and La3+ from a Ca-La- calmodulin complex (Ca2La2CaM).展开更多
As a significant concept in the field of philosophy of logic, identity plays a key role in Quine's Ontological Commitment Theory. Quine uses the quantitative method of predicate logic to study the ontological prob...As a significant concept in the field of philosophy of logic, identity plays a key role in Quine's Ontological Commitment Theory. Quine uses the quantitative method of predicate logic to study the ontological problem, and puts forward the ontological commitment standard that "To be is to be the value of a variable". The bound variable becomes the only device that undertakes the ontological commitment ftinction. The value of the bound variable is the entity of the ontological commitment made by the theory in which it is based. However, Quine argues, not all objects can be the values of the bound variable and thus the entities that the theory promises to exist. Quine takes the concept of identity as a necessary condition of ontological commitment, that is,"no identity, no entity". In Quine's view, the concept of identity refers to the identity between extended objects, rather than the identity between connotative objects and possible objects. As the core concept of Ontological Commitment Theory, the concept of identity has a profound influence on Quine's philosophy.展开更多
Quine＇s justly famous paper ＂On What There Is＂ introduced a criterion of ontological commitment which has been almost universally accepted by analytic philosophers ever since. In this paper 1 try to unpack some of ...Quine＇s justly famous paper ＂On What There Is＂ introduced a criterion of ontological commitment which has been almost universally accepted by analytic philosophers ever since. In this paper 1 try to unpack some of the substantive and controversial philosophical commitments that are presupposed by this criterion. The aim is not to show that the criterion is incorrect, but merely that it is not as obvious as it is taken to be by many, and that we might have reasons to explore alternative ways of thinking about ontological commitments.展开更多
The intracellular free calcium concentration plays a key role as the second messenger in the regulation of cellular reaction.The changes of cytoplasmic free calcium concentration([Ca^(2+)]_i) in human platelets loaded...The intracellular free calcium concentration plays a key role as the second messenger in the regulation of cellular reaction.The changes of cytoplasmic free calcium concentration([Ca^(2+)]_i) in human platelets loaded with the fluorescent calcium indicator quin-2 were measured in this study. The result showed that thrombin(0.3 U/ml)raised the[Ca^(2+)]_i of platelets four to five times in the presence of 1 mmol/L external calcium.but there was a much smaller increase in[Ca^(2+)]_i in the absence of external calcium.It seems that thrombin increased[Ca^(2+)]_i partly by release of internal Ca^(2+),but mainly by stimulating influx.J-894 markedly inhibited the rise of[Ca^(2+)]_i in platelets induced by thrombin in a dose dependent manner through decreasing both calcium influx and calcium release.This suggests that J-894 may be a calcium channel blocker.展开更多
The current corona-2 pandemic has stimulated wide research for hydroxychloroquine(Quine)therapy and lately,prophylaxis.To optimize prophylaxis proper methods of use are explained.The focus is on tools of assessment an...The current corona-2 pandemic has stimulated wide research for hydroxychloroquine(Quine)therapy and lately,prophylaxis.To optimize prophylaxis proper methods of use are explained.The focus is on tools of assessment and robust comparison;defining infection objectively;loading and maintenance dose designing based on pharmaco-viro-kinetics;confirming Quine threshold-levels and its sufficiency;and Quine side-effects vigilance/amelioration.Attention to statistics to study valid endpoints of goals in appropriately-sized population is essential.Mass interactive quine dose auto designer software is built to simplify,optimize and help collaboration of complex Quine dosing system.A similar chloroquine software can be built.展开更多
文摘The fluorescence spectra of Quin 2, (2-[(2-bis-[carboxymethyl] amino-5-methylphenoxy) methyl]-6-methoxy-8-bis [carboxymethyl] aminoquiniline), a Ca2+ probe, were investigated upon incubation with Ca2+ or La3+. The results showed that binding of La3+ to Quin 2 resulted in different fluorescent spectrum from that of Ca2+. Based on this observation, a fluorescent method was developed for simultaneously determination of the dissociation rates of Ca2+ and La3+ from a Ca-La- calmodulin complex (Ca2La2CaM).
基金the Major Project of the Humanities and Social Science Foundation of Educational Commission of Hebei Province (ZD201818).
文摘As a significant concept in the field of philosophy of logic, identity plays a key role in Quine's Ontological Commitment Theory. Quine uses the quantitative method of predicate logic to study the ontological problem, and puts forward the ontological commitment standard that "To be is to be the value of a variable". The bound variable becomes the only device that undertakes the ontological commitment ftinction. The value of the bound variable is the entity of the ontological commitment made by the theory in which it is based. However, Quine argues, not all objects can be the values of the bound variable and thus the entities that the theory promises to exist. Quine takes the concept of identity as a necessary condition of ontological commitment, that is,"no identity, no entity". In Quine's view, the concept of identity refers to the identity between extended objects, rather than the identity between connotative objects and possible objects. As the core concept of Ontological Commitment Theory, the concept of identity has a profound influence on Quine's philosophy.
文摘Quine＇s justly famous paper ＂On What There Is＂ introduced a criterion of ontological commitment which has been almost universally accepted by analytic philosophers ever since. In this paper 1 try to unpack some of the substantive and controversial philosophical commitments that are presupposed by this criterion. The aim is not to show that the criterion is incorrect, but merely that it is not as obvious as it is taken to be by many, and that we might have reasons to explore alternative ways of thinking about ontological commitments.
文摘The intracellular free calcium concentration plays a key role as the second messenger in the regulation of cellular reaction.The changes of cytoplasmic free calcium concentration([Ca^(2+)]_i) in human platelets loaded with the fluorescent calcium indicator quin-2 were measured in this study. The result showed that thrombin(0.3 U/ml)raised the[Ca^(2+)]_i of platelets four to five times in the presence of 1 mmol/L external calcium.but there was a much smaller increase in[Ca^(2+)]_i in the absence of external calcium.It seems that thrombin increased[Ca^(2+)]_i partly by release of internal Ca^(2+),but mainly by stimulating influx.J-894 markedly inhibited the rise of[Ca^(2+)]_i in platelets induced by thrombin in a dose dependent manner through decreasing both calcium influx and calcium release.This suggests that J-894 may be a calcium channel blocker.
文摘The current corona-2 pandemic has stimulated wide research for hydroxychloroquine(Quine)therapy and lately,prophylaxis.To optimize prophylaxis proper methods of use are explained.The focus is on tools of assessment and robust comparison;defining infection objectively;loading and maintenance dose designing based on pharmaco-viro-kinetics;confirming Quine threshold-levels and its sufficiency;and Quine side-effects vigilance/amelioration.Attention to statistics to study valid endpoints of goals in appropriately-sized population is essential.Mass interactive quine dose auto designer software is built to simplify,optimize and help collaboration of complex Quine dosing system.A similar chloroquine software can be built.
