Viral diseases are an important threat to crop yield,as they are responsible for losses greater than US$30 billion annually.Thus,understanding the dynamics of virus propagation within plant cells is essential for devi...Viral diseases are an important threat to crop yield,as they are responsible for losses greater than US$30 billion annually.Thus,understanding the dynamics of virus propagation within plant cells is essential for devising effective control strategies.However,viruses are complex to propagate and quantify.Existing methodologies for viral quantification tend to be expensive and time-consuming.Here,we present a rapid cost-effective approach to quantify viral propagation using an engineered virus expressing a fluorescent reporter.Using a microplate reader,we measured viral protein levels and we validated our findings through comparison by western blot analysis of viral coat protein,the most common approach to quantify viral titer.Our proposed methodology provides a practical and accessible approach to studying virus-host interactions and could contribute to enhancing our understanding of plant virology.展开更多
BACKGROUND The Streptococcus salivarius(S.salivarius)group,which produces the enzyme urease has been identified as a potential contributor to ammonia production in the gut.Researchers have reported that patients with ...BACKGROUND The Streptococcus salivarius(S.salivarius)group,which produces the enzyme urease has been identified as a potential contributor to ammonia production in the gut.Researchers have reported that patients with minimal HE had an increased abundance of the S.salivarius group,which is a specific change in the gut microbiota that distinguishes them from healthy individuals.The correlation between the aggregation of specific bacterial species and fibrosis progression in chronic liver disease(CLD)is yet to be fully elucidated.AIM To quantify S.salivarius using digital PCR(dPCR)as a liver fibrosis marker of CLD.METHODS This study retrospectively analysed 52 patients with CLD.To quantify S.salivarius in patients with CLD using dPCR,we evaluated the specificity and sensitivity of S.salivarius bacterial load using dPCR for a type strain.Next,we evaluated the clinical usefulness of dPCR for S.salivarius load quantification for detecting liver fibrosis in patients with CLD.The liver fibrosis stage was categorized into mild and advanced fibrosis based on pathological findings.RESULTS The dPCR assay revealed that S.salivarius was highly positive for the tnpA gene.The lower limit of quantification for dPCR using the tnpA gene with a 1μL template comprising 1.28×102 CFU/mL was 4.3 copies.After considering the detection range in dPCR,we adjusted the extracted DNA concentration to 5.0×10-4 ng/μL from 200 mg stool samples.The median bacterial loads of S.salivarius in stool sample from patients with mild and advanced fibrosis were 1.9 and 7.4 copies/μL,respectively.The quantification of S.salivarius load was observed more frequently in patients with advanced fibrosis than in those with mild fibrosis(P=0.032).CONCLUSION Quantifying of S.salivarius load using digital PCR is a useful biomarker for liver fibrosis in patients with CLD.展开更多
During high-speed forward flight,helicopter rotor blades operate across a wide range of Reynolds and Mach numbers.Under such conditions,their aerodynamic performance is significantly influenced by dynamic stall—a com...During high-speed forward flight,helicopter rotor blades operate across a wide range of Reynolds and Mach numbers.Under such conditions,their aerodynamic performance is significantly influenced by dynamic stall—a complex,unsteady flow phenomenon highly sensitive to inlet conditions such asMach and Reynolds numbers.The key features of three-dimensional blade stall can be effectively represented by the dynamic stall behavior of a pitching airfoil.In this study,we conduct an uncertainty quantification analysis of dynamic stall aerodynamics in high-Mach-number flows over pitching airfoils,accounting for uncertainties in inlet parameters.A computational fluid dynamics(CFD)model based on the compressible unsteady Reynolds-averagedNavier–Stokes(URANS)equations,coupledwith sliding mesh techniques,is developed to simulate the unsteady aerodynamic behavior and associated flow fields.To efficiently capture the aerodynamic responses while maintaining high accuracy,a multi-fidelity Co-Kriging surrogate model is constructed.This model integrates the precision of high-fidelity wind tunnel experiments with the computational efficiency of lower-fidelity URANS simulations.Its accuracy is validated through direct comparison with experimental data.Building upon this surrogate model,we employ interval analysis and the Sobol sensitivity method to quantify the uncertainty and parameter sensitivity of the unsteady aerodynamic forces resulting frominlet condition variability.Both the inlet Mach number and Reynolds number are treated as uncertain inputs,modeled using interval representations.Our results demonstrate that variations inMach number contribute far more significantly to aerodynamic uncertainty than those in Reynolds number.Moreover,the presence of dynamic stall vortices markedly amplifies the aerodynamic sensitivity to Mach number fluctuations.展开更多
Excessive Fe^(3+) ion concentrations in wastewater pose a long-standing threat to human health.Achieving low-cost,high-efficiency quantification of Fe^(3+) ion concentration in unknown solutions can guide environmenta...Excessive Fe^(3+) ion concentrations in wastewater pose a long-standing threat to human health.Achieving low-cost,high-efficiency quantification of Fe^(3+) ion concentration in unknown solutions can guide environmental management decisions and optimize water treatment processes.In this study,by leveraging the rapid,real-time detection capabilities of nanopores and the specific chemical binding affinity of tannic acid to Fe^(3+),a linear relationship between the ion current and Fe^(3+) ion concentration was established.Utilizing this linear relationship,quantification of Fe^(3+) ion concentration in unknown solutions was achieved.Furthermore,ethylenediaminetetraacetic acid disodium salt was employed to displace Fe^(3+) from the nanopores,allowing them to be restored to their initial conditions and reused for Fe^(3+) ion quantification.The reusable bioinspired nanopores remain functional over 330 days of storage.This recycling capability and the long-term stability of the nanopores contribute to a significant reduction in costs.This study provides a strategy for the quantification of unknown Fe^(3+) concentration using nanopores,with potential applications in environmental assessment,health monitoring,and so forth.展开更多
In the data transaction process within a data asset trading platform,quantifying the trustworthiness of data source nodes is challenging due to their numerous attributes and complex structures.To address this issue,a ...In the data transaction process within a data asset trading platform,quantifying the trustworthiness of data source nodes is challenging due to their numerous attributes and complex structures.To address this issue,a distributed data source trust assessment management framework,a trust quantification model,and a dynamic adjustment mechanism are proposed.Themodel integrates the Analytic Hierarchy Process(AHP)and Dempster-Shafer(D-S)evidence theory to determine attribute weights and calculate direct trust values,while the PageRank algorithm is employed to derive indirect trust values.Thedirect and indirect trust values are then combined to compute the comprehensive trust value of the data source.Furthermore,a dynamic adjustment mechanism is introduced to continuously update the comprehensive trust value based on historical assessment data.By leveraging the collaborative efforts of multiple nodes in the distributed network,the proposed framework enables a comprehensive,dynamic,and objective evaluation of data source trustworthiness.Extensive experimental analyses demonstrate that the trust quantification model effectively handles large-scale data source trust assessments,exhibiting both strong trust differentiation capability and high robustness.展开更多
For uncertainty quantification of complex models with high-dimensional,nonlinear,multi-component coupling like digital twins,traditional statistical sampling methods,such as random sampling and Latin hypercube samplin...For uncertainty quantification of complex models with high-dimensional,nonlinear,multi-component coupling like digital twins,traditional statistical sampling methods,such as random sampling and Latin hypercube sampling,require a large number of samples,which entails huge computational costs.Therefore,how to construct a small-size sample space has been a hot issue of interest for researchers.To this end,this paper proposes a sequential search-based Latin hypercube sampling scheme to generate efficient and accurate samples for uncertainty quantification.First,the sampling range of the samples is formed by carving the polymorphic uncertainty based on theoretical analysis.Then,the optimal Latin hypercube design is selected using the Latin hypercube sampling method combined with the"space filling"criterion.Finally,the sample selection function is established,and the next most informative sample is optimally selected to obtain the sequential test sample.Compared with the classical sampling method,the generated samples can retain more information on the basis of sparsity.A series of numerical experiments are conducted to demonstrate the superiority of the proposed sequential search-based Latin hypercube sampling scheme,which is a way to provide reliable uncertainty quantification results with small sample sizes.展开更多
Quantitative analysis of clinical function parameters from MRI images is crucial for diagnosing and assessing cardiovascular disease.However,the manual calculation of these parameters is challenging due to the high va...Quantitative analysis of clinical function parameters from MRI images is crucial for diagnosing and assessing cardiovascular disease.However,the manual calculation of these parameters is challenging due to the high variability among patients and the time-consuming nature of the process.In this study,the authors introduce a framework named MultiJSQ,comprising the feature presentation network(FRN)and the indicator prediction network(IEN),which is designed for simultaneous joint segmentation and quantification.The FRN is tailored for representing global image features,facilitating the direct acquisition of left ventricle(LV)contour images through pixel classification.Additionally,the IEN incorporates specifically designed modules to extract relevant clinical indices.The authors’method considers the interdependence of different tasks,demonstrating the validity of these relationships and yielding favourable results.Through extensive experiments on cardiac MR images from 145 patients,MultiJSQ achieves impressive outcomes,with low mean absolute errors of 124 mm^(2),1.72 mm,and 1.21 mm for areas,dimensions,and regional wall thicknesses,respectively,along with a Dice metric score of 0.908.The experimental findings underscore the excellent performance of our framework in LV segmentation and quantification,highlighting its promising clinical application prospects.展开更多
Wheat powdery mildew caused by Blumeria graminis f.sp.tritici(Bgt)is an important disease worldwide.Detection of latent infection of leaves by the pathogen in late autumn is valuable for estimating the inoculum potent...Wheat powdery mildew caused by Blumeria graminis f.sp.tritici(Bgt)is an important disease worldwide.Detection of latent infection of leaves by the pathogen in late autumn is valuable for estimating the inoculum potential to assess disease risks in the spring.We developed a new tool for rapid detection and quantification of latent infection of seedlings by the pathogen.The method was based on recombinase polymerase amplification(RPA)coupled with an end-point detection via lateral flow device(LFD).The limit of detection is 100 agμL^(-1)of Bgt DNA,without noticeable interference from either other common wheat pathogens or wheat material(Triticum aestivum).It was evaluated on wheat seedlings for this accuracy and sensitivity in detecting latent infection of Bgt.We further extended this RPALFD assay to estimate the level of latent infection by Bgt based on imaging analysis.There was a strong correlation between the image-based and real-time PCR assay estimates of Bgt DNA.The present results suggested that this new tool can provide rapid and accurate quantification of Bgt in latently infected leaves and can be further development as an on-site monitoring tool.展开更多
Abstract： In the present study, we established an ultra performance liquid chromatography coupled with time-of-flight mass spectrometry （UPLC-QTOF-MSE） method to simultaneously quantify 33 components in Ginkgo bilo...Abstract： In the present study, we established an ultra performance liquid chromatography coupled with time-of-flight mass spectrometry （UPLC-QTOF-MSE） method to simultaneously quantify 33 components in Ginkgo biloba leaf extracts （GBEs）, including 17 flavonol glycosides, five terpene trilactones （TTLs）, four polyphenols and seven carboxylic acids. This optimized method was successfully applied to analyze the explicit compositions of GBE samples collected from different places. Furthermore, the data were processed through unsupervised principal component analysis （PCA） and supervised orthogonal partial least squared discrimination analysis （OPLS-DA） to evaluate the quality and compare the differences between the samples according to the contents of the 33 chemical constituents. Bilobalide, protocatechuic acid, shikimic acid, quinic acid, ginkgolide B, ginkgolide J, kaempferol-3-O-rutinoside, isorhamnetin-3-O-rutinoside, quercetin-3-O-ct-L-rhamnopyranocyl-2＂-（6＇＂-p-coumaroyl）-β-D-glucoside and rutin were recognized as characteristic chemical markers that contributed most to control the quality of GBEs. Based on the fact that GBEs should be standardized with the characteristic components as quality control chemical markers, it is most important to maintain the quality of GBEs stable and reliable, and this method also provided a good strategy to further rectify and standardize the GBEs market.展开更多
In the present study, we developed and validated a high-performance liquid chromatography method for the simultaneous determination of seven phenylpropanoid compounds （2-hydroxyl cinnamaldehyde, coumarin, cinnamyl al...In the present study, we developed and validated a high-performance liquid chromatography method for the simultaneous determination of seven phenylpropanoid compounds （2-hydroxyl cinnamaldehyde, coumarin, cinnamyl alcohol, cinnamic acid, 2-methoxy cinnamic acid, cinnamaldehyde and 2-methoxy cinnamaldehyde） in Cinnamomi Cortex and Cinnamomi Ramulus. The levels of seven phenylpropanoid compounds in Cinnamomi Cortex and Cinnamomi Ramulus were compared using this method. A total of 48 samples （27 Cinnamomi Cortex and 21 Cinnamomi Ramulus） were purchased in China and analyzed. Quantities of seven phenylpropanoid compounds ranged from 17.5 to 61.6 mg/g in Cinnamomi Cortex and ranged from 9.91 to 23.4 mg/g in Ciunamomi Ramulus. The level of 2-methoxy cinnamic acid in the Cinnamomi Cortex samples was below the LOD, whereas it ranged from 0 to 0.119 mg/g in the Cinnamomi Ramulus samples. The （cinnamyl alcohol＋cinnamic acid）/cinnamaldehyde ratios （R346） of Ciunamomi Cortex and Cinnamomi Ramulus ranged from 0.0121 to 0.0467 and 0.0598 to 0.182, respectively. This ratio could be used to discriminate Cinnamomi Cortex （〈0.05） and Cinnamomi Ramulus （〉0.05）. The extraction rates （Dn） of seven compounds in boiling water were different, with the lowest dissolution for cinnamaldehyde （〈3%） and the highest for cinnamic acid （about 60%）.展开更多
For the first time, we have utilized high-performance liquid chromatography （HPLC） to simultaneously quantify the eugenol and bancroffione in Caryophylli Fructus. The optimized parameters included： Inertsil ODS-4 c...For the first time, we have utilized high-performance liquid chromatography （HPLC） to simultaneously quantify the eugenol and bancroffione in Caryophylli Fructus. The optimized parameters included： Inertsil ODS-4 column （150 mm×4.6 mm, 5 μm）; column temperature： 35 ℃; mobile phase： methanol water （65：35, v/v）; flow rate： 1.0 mL/min; detection wavelength： 280 nm. Eugenol and bancroftione showed good linear relationships with peak areas within the range of （0.0998 0.8982） mg/mL （r = 0.9999） and （0.1474-1.3266） mg/mL （r = 0.9999）, respectively. The average recoveries were 102.52% and 100.96% for eugenol and bancroftione, respectively. Our results showed that the established method is simple, rapid, and accurate with good reproducibility to evaluate the quality of Caryophylli Fructus.展开更多
High-performance liquid chromatography with UV detection was used for the quantification of the flavonoid quercetin, the active compound found in ＂Guangdong Wang-bu-liu-xing＂. The method was developed and demonstrat...High-performance liquid chromatography with UV detection was used for the quantification of the flavonoid quercetin, the active compound found in ＂Guangdong Wang-bu-liu-xing＂. The method was developed and demonstrated to provide superior performance over other commonly documented methods. This HPLC assay achieved high specificity through the use of a reversed-phase C18 column eluted with a mobile phase of methanol-0.4% （v/v） phosphoric acid （50：50, v/v） over the course of 30 min. UV detection at 360 nm was used. This analytical method provided excellent precision and a mean recovery of 99%, demonstrated by repeated analysis of 11 sample groups. Because of its high performance and simplicity, this HPLC assay can be readily utilized as a practical method for the quality control of active compounds extracted from Guangdong Wang-bu-liu-xing.展开更多
Real-time PCR is a closed DNA amplification system that skillfully integrates biochemical, photoelectric and computer techniques. Fluorescence data acquired once per cycle provides rapid absolute quantification of ini...Real-time PCR is a closed DNA amplification system that skillfully integrates biochemical, photoelectric and computer techniques. Fluorescence data acquired once per cycle provides rapid absolute quantification of initial template copy numbers as PCR products are generated. This technique significantly simplifies and accelerates the process of producing reproducible quantification of nucleic acid molecules. It not only is a sensitive, accurate and rapid quantitative method, but it also provides an easier way to calculate the absolute starting copy number of nucleic acid molecules to be tested. Together with molecular bio-techniques, like microarray, real-time PCR will play a very important role in many aspects of molecular life science such as functional gene analysis and disease molecular diagnostics. This review introduces the detailed principles and application of the real-time PCR technique, describes a recently developed system for exact quantification of AUX/IAA genes In Arabidopsis, and discusses the problems with the real-time PCR process.展开更多
This paper investigates the influence of crack orientation on damage quantification using Lamb wave in plate structures. Finite element simulation is performed to acquire Lamb wave signal responses for different confi...This paper investigates the influence of crack orientation on damage quantification using Lamb wave in plate structures. Finite element simulation is performed to acquire Lamb wave signal responses for different configurations of crack orientations and crack lengths. Two Lamb wave features, namely the normalized amplitude and the phase change, are used as damage sensitive features to develop a crack size quantification model. A hypothesis based on the geometrical influence on signal features is proposed, and the crack size quantification model incorporating the orientation angle is established using the hypothesis. An index of Probability of Reliable Quantification(PRQ) is proposed to evaluate the performance of the model. The index can be used to determine the sizing risk in terms of probabilities. A realistic aluminum plate is used to obtain the experimental data using piezoelectric(PZT) wafer-type sensors around a center through crack. The experimental data are used to validate the overall method. Results indicate that the proposed model can yield reliable results for size quantification of inclined cracks.展开更多
AIM To investigate and compare the analytical and clinical performance of Tian Long automatic hypersensitive hepatitis B virus(HBV) DNA quantification system and Roche CAP/CTM system.METHODS Two hundred blood samples ...AIM To investigate and compare the analytical and clinical performance of Tian Long automatic hypersensitive hepatitis B virus(HBV) DNA quantification system and Roche CAP/CTM system.METHODS Two hundred blood samples for HBV DNA testing, HBV-DNA negative samples and high-titer HBV-DNA mixture samples were collected and prepared. National standard materials for serum HBV and a worldwide HBV DNA panel were employed for performance verification. The analytical performance, such as limit of detection, limit of quantification, accuracy, precision, reproducibility, linearity, genotype coverage and cross-contamination, was determined using the Tian Long automatic hypersensitive HBV DNA quantification system(TL system). Correlation and Bland-Altman plot analyses were carried out to compare the clinical performance of the TL system assay and the CAP/CTM system. RESULTS The detection limit of the TL system was 10 IU/m L, and its limit of quantification was 30 IU/m L. The differences between the expected and tested concentrations of the national standards were less than ± 0.4 Log10 IU/m L, which showed high accuracy of the system. Results of the precision, reproducibility and linearity tests showed that the multiple test coefficient of variation(CV) of the same sample was less than 5% for 102-106 IU/m L; and for 30-108 IU/m L, the linear correlation coefficient r2 = 0.99. The TL system detected HBV DNA(A-H) genotypes and there was no cross-contamination during the "checkerboard" test. When compared with the CAP/CTM assay, the two assays showed 100% consistency in both negative and positive sample results(15 negative samples and 185 positive samples). No statistical differences between the two assays in the HBV DNA quantification values were observed(P > 0.05). Correlation analysis indicated a significant correlation between the two assays, r2 = 0.9774. The Bland-Altman plot analysis showed that 98.9% of the positive data were within the 95% acceptable range, and the maximum difference was-0.49.CONCLUSION The TL system has good analytical performance, and exhibits good agreement with the CAP/CTM system in clinical performance.展开更多
The regional hydrological system is extremely complex because it is affected not only by physical factors but also by human dimensions.And the hydrological models play a very important role in simulating the complex s...The regional hydrological system is extremely complex because it is affected not only by physical factors but also by human dimensions.And the hydrological models play a very important role in simulating the complex system.However,there have not been effective methods for the model reliability and uncertainty analysis due to its complexity and difficulty.The uncertainties in hydrological modeling come from four important aspects：uncertainties in input data and parameters,uncertainties in model structure,uncertainties in analysis method and the initial and boundary conditions.This paper systematically reviewed the recent advances in the study of the uncertainty analysis approaches in the large-scale complex hydrological model on the basis of uncertainty sources.Also,the shortcomings and insufficiencies in the uncertainty analysis for complex hydrological models are pointed out.And then a new uncertainty quantification platform PSUADE and its uncertainty quantification methods were introduced,which will be a powerful tool and platform for uncertainty analysis of large-scale complex hydrological models.Finally,some future perspectives on uncertainty quantification are put forward.展开更多
The present study was designed to investigate the bioactive constituents ofXanthoceras sorbifolia in terms of amounts and their antioxidant, DNA scissiou protection, and ct-glucosidase inhibitory activities. Simultane...The present study was designed to investigate the bioactive constituents ofXanthoceras sorbifolia in terms of amounts and their antioxidant, DNA scissiou protection, and ct-glucosidase inhibitory activities. Simultaneous quantification of 10 X. sorbifolia constituents was carried out by a newly established ultra-high performance liquid chromatography-quadrupole mass spectrometry method （UHPLC-MS）, The antioxidant activities were evaluated by measuring DPPH radical scavenging and DNA scission protective activities. The a-glucosidase inhibitory activities were investigated by using an assay with a-glucosidase from Bacillus Stearothermophilus and disaccharidases from mouse intestine. We found that the wood ofX. sorbifolia was rich in phenolic compounds with the contents of catechin, epicatechin, myricetin, and dihydromyricetin being 0.12-0.19, 1.94-2.16, 0.77-0.91, and 6.76-7.89 mg.g-1, respectively. The four constituents strongly scavenged DPPH radicals （with ECs0 being 4.2, 3.8 and 5.7 μg-mL-1, respectively） and remarkably protected peroxyl radical-induced DNA strand scission （92.10%, 94.66%, 75.44% and 89.95% of protection, respectively, at a concentration of 10 μmol.L-1）. A dimeric flavan 3-ol, epigallocatechin-（4β→8, 2β→O-7）-epicatechin potently inhibited a-glucosidase with an IC50 value being as low as 1.2 μg.mL1. The established UHPLC-MS method could serve as a quality control tool for X. sorbifolia. In conclusion, the high contents of antioxidant and α-glucosidase inhibitory constituents in X. sorbifolia support its use as complementation of other therapeutic agents for metabolic disorders, such as diabetes and hypertension.展开更多
Manufactured blades are inevitably different from their design intent,which leads to a deviation of the performance from the intended value.To quantify the associated performance uncertainty,many approaches have been ...Manufactured blades are inevitably different from their design intent,which leads to a deviation of the performance from the intended value.To quantify the associated performance uncertainty,many approaches have been developed.The traditional Monte Carlo method based on a Computational Fluid Dynamics solver(MC-CFD)for a three-dimensional compressor is prohibitively expensive.Existing alternatives to the MC-CFD,such as surrogate models and secondorder derivatives based on the adjoint method,can greatly reduce the computational cost.Nevertheless,they will encounter’the curse of dimensionality’except for the linear model based on the adjoint gradient(called MC-adj-linear).However,the MC-adj-linear model neglects the nonlinearity of the performance function.In this work,an improved method is proposed to circumvent the lowaccuracy problem of the MC-adj-linear without incurring the high cost of other alternative models.The method is applied to the study of the aerodynamic performance of an annular transonic compressor cascade,subject to prescribed geometric variability with industrial relevance.It is found that the proposed method achieves a significant accuracy improvement over the MC-adj-linear with low computational cost,showing the great potential for fast uncertainty quantification.展开更多
This paper reviews recent research on the demand flexibility of residential buildings in regard to definitions,flexible loads,and quantification methods.A systematic distinction of the terminology is made,including th...This paper reviews recent research on the demand flexibility of residential buildings in regard to definitions,flexible loads,and quantification methods.A systematic distinction of the terminology is made,including the demand flexibility,operation flexibility,and energy flexibility of buildings.A comprehensive definition of building demand flexibility is proposed based on an analysis of the existing definitions.Moreover,the flexibility capabilities and operation characteristics of the main residential flexible loads are summarized and compared.Models and evaluation indicators to quantify the flexibility of these flexible loads are reviewed and summarized.Current research gaps and challenges are identified and analyzed as well.The results indicate that previous studies have focused on the flexibility of central air conditioning,electric water heaters,wet appliances,refrigerators,and lighting,where the proportion of studies focusing on each of these subjects is 36.7%,25.7%,14.7%,9.2%,and 8.3%,respectively.These flexible loads are different in running modes,usage frequencies,seasons,and capabilities for shedding,shifting,and modulation,while their response characteristics are not yet clear.Furthermore,recommendations are given for the application of white-,black-,and grey-box models for modeling flexible loads in different situations.Numerous static flexibility evaluation indicators that are based on the aspects of power,temporality,energy,efficiency,economics,and the environment have been proposed in previous publications,but a consensus and standardized evaluation framework is lacking.This review can help readers better understand building demand flexibility and learn about the characteristics of different residential flexible loads,while also providing suggestions for future research on the modeling techniques and evaluation metrics of residential building demand flexibility.展开更多
基金Funding from Natural Sciences and Engineering Research Council of Canada award number RGPIN/4002-2020.
文摘Viral diseases are an important threat to crop yield,as they are responsible for losses greater than US$30 billion annually.Thus,understanding the dynamics of virus propagation within plant cells is essential for devising effective control strategies.However,viruses are complex to propagate and quantify.Existing methodologies for viral quantification tend to be expensive and time-consuming.Here,we present a rapid cost-effective approach to quantify viral propagation using an engineered virus expressing a fluorescent reporter.Using a microplate reader,we measured viral protein levels and we validated our findings through comparison by western blot analysis of viral coat protein,the most common approach to quantify viral titer.Our proposed methodology provides a practical and accessible approach to studying virus-host interactions and could contribute to enhancing our understanding of plant virology.
文摘BACKGROUND The Streptococcus salivarius(S.salivarius)group,which produces the enzyme urease has been identified as a potential contributor to ammonia production in the gut.Researchers have reported that patients with minimal HE had an increased abundance of the S.salivarius group,which is a specific change in the gut microbiota that distinguishes them from healthy individuals.The correlation between the aggregation of specific bacterial species and fibrosis progression in chronic liver disease(CLD)is yet to be fully elucidated.AIM To quantify S.salivarius using digital PCR(dPCR)as a liver fibrosis marker of CLD.METHODS This study retrospectively analysed 52 patients with CLD.To quantify S.salivarius in patients with CLD using dPCR,we evaluated the specificity and sensitivity of S.salivarius bacterial load using dPCR for a type strain.Next,we evaluated the clinical usefulness of dPCR for S.salivarius load quantification for detecting liver fibrosis in patients with CLD.The liver fibrosis stage was categorized into mild and advanced fibrosis based on pathological findings.RESULTS The dPCR assay revealed that S.salivarius was highly positive for the tnpA gene.The lower limit of quantification for dPCR using the tnpA gene with a 1μL template comprising 1.28×102 CFU/mL was 4.3 copies.After considering the detection range in dPCR,we adjusted the extracted DNA concentration to 5.0×10-4 ng/μL from 200 mg stool samples.The median bacterial loads of S.salivarius in stool sample from patients with mild and advanced fibrosis were 1.9 and 7.4 copies/μL,respectively.The quantification of S.salivarius load was observed more frequently in patients with advanced fibrosis than in those with mild fibrosis(P=0.032).CONCLUSION Quantifying of S.salivarius load using digital PCR is a useful biomarker for liver fibrosis in patients with CLD.
文摘During high-speed forward flight,helicopter rotor blades operate across a wide range of Reynolds and Mach numbers.Under such conditions,their aerodynamic performance is significantly influenced by dynamic stall—a complex,unsteady flow phenomenon highly sensitive to inlet conditions such asMach and Reynolds numbers.The key features of three-dimensional blade stall can be effectively represented by the dynamic stall behavior of a pitching airfoil.In this study,we conduct an uncertainty quantification analysis of dynamic stall aerodynamics in high-Mach-number flows over pitching airfoils,accounting for uncertainties in inlet parameters.A computational fluid dynamics(CFD)model based on the compressible unsteady Reynolds-averagedNavier–Stokes(URANS)equations,coupledwith sliding mesh techniques,is developed to simulate the unsteady aerodynamic behavior and associated flow fields.To efficiently capture the aerodynamic responses while maintaining high accuracy,a multi-fidelity Co-Kriging surrogate model is constructed.This model integrates the precision of high-fidelity wind tunnel experiments with the computational efficiency of lower-fidelity URANS simulations.Its accuracy is validated through direct comparison with experimental data.Building upon this surrogate model,we employ interval analysis and the Sobol sensitivity method to quantify the uncertainty and parameter sensitivity of the unsteady aerodynamic forces resulting frominlet condition variability.Both the inlet Mach number and Reynolds number are treated as uncertain inputs,modeled using interval representations.Our results demonstrate that variations inMach number contribute far more significantly to aerodynamic uncertainty than those in Reynolds number.Moreover,the presence of dynamic stall vortices markedly amplifies the aerodynamic sensitivity to Mach number fluctuations.
基金supported by the National Natural Science Foundation of China(Nos.52303380,52025132,52273305,22205185,21621091,22021001,and 22121001)Fundamental Research Funds for the Central Universities(No.20720240041)+3 种基金the 111 Project(Nos.B17027 and B16029)the National Science Foundation of Fujian Province of China(No.2022J02059)the Science and Technology Projects of Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province(No.RD2022070601)the New Cornerstone Science Foundation through the XPLORER PRIZE。
文摘Excessive Fe^(3+) ion concentrations in wastewater pose a long-standing threat to human health.Achieving low-cost,high-efficiency quantification of Fe^(3+) ion concentration in unknown solutions can guide environmental management decisions and optimize water treatment processes.In this study,by leveraging the rapid,real-time detection capabilities of nanopores and the specific chemical binding affinity of tannic acid to Fe^(3+),a linear relationship between the ion current and Fe^(3+) ion concentration was established.Utilizing this linear relationship,quantification of Fe^(3+) ion concentration in unknown solutions was achieved.Furthermore,ethylenediaminetetraacetic acid disodium salt was employed to displace Fe^(3+) from the nanopores,allowing them to be restored to their initial conditions and reused for Fe^(3+) ion quantification.The reusable bioinspired nanopores remain functional over 330 days of storage.This recycling capability and the long-term stability of the nanopores contribute to a significant reduction in costs.This study provides a strategy for the quantification of unknown Fe^(3+) concentration using nanopores,with potential applications in environmental assessment,health monitoring,and so forth.
基金funded by Haikou Science and Technology Plan Project(2022-007),in part by key Laboratory of PK System Technologies Research of Hainan,China.
文摘In the data transaction process within a data asset trading platform,quantifying the trustworthiness of data source nodes is challenging due to their numerous attributes and complex structures.To address this issue,a distributed data source trust assessment management framework,a trust quantification model,and a dynamic adjustment mechanism are proposed.Themodel integrates the Analytic Hierarchy Process(AHP)and Dempster-Shafer(D-S)evidence theory to determine attribute weights and calculate direct trust values,while the PageRank algorithm is employed to derive indirect trust values.Thedirect and indirect trust values are then combined to compute the comprehensive trust value of the data source.Furthermore,a dynamic adjustment mechanism is introduced to continuously update the comprehensive trust value based on historical assessment data.By leveraging the collaborative efforts of multiple nodes in the distributed network,the proposed framework enables a comprehensive,dynamic,and objective evaluation of data source trustworthiness.Extensive experimental analyses demonstrate that the trust quantification model effectively handles large-scale data source trust assessments,exhibiting both strong trust differentiation capability and high robustness.
基金co-supported by the National Natural Science Foundation of China(Nos.51875014,U2233212 and 51875015)the Natural Science Foundation of Beijing Municipality,China(No.L221008)+1 种基金Science,Technology Innovation 2025 Major Project of Ningbo of China(No.2022Z005)the Tianmushan Laboratory Project,China(No.TK2023-B-001)。
文摘For uncertainty quantification of complex models with high-dimensional,nonlinear,multi-component coupling like digital twins,traditional statistical sampling methods,such as random sampling and Latin hypercube sampling,require a large number of samples,which entails huge computational costs.Therefore,how to construct a small-size sample space has been a hot issue of interest for researchers.To this end,this paper proposes a sequential search-based Latin hypercube sampling scheme to generate efficient and accurate samples for uncertainty quantification.First,the sampling range of the samples is formed by carving the polymorphic uncertainty based on theoretical analysis.Then,the optimal Latin hypercube design is selected using the Latin hypercube sampling method combined with the"space filling"criterion.Finally,the sample selection function is established,and the next most informative sample is optimally selected to obtain the sequential test sample.Compared with the classical sampling method,the generated samples can retain more information on the basis of sparsity.A series of numerical experiments are conducted to demonstrate the superiority of the proposed sequential search-based Latin hypercube sampling scheme,which is a way to provide reliable uncertainty quantification results with small sample sizes.
基金Hefei Municipal Natural Science Foundation,Grant/Award Number:2022009Suqian Guiding Program Project,Grant/Award Number:Z202309Suqian Traditional Chinese Medicine Science and Technology Plan,Grant/Award Number:MS202301。
文摘Quantitative analysis of clinical function parameters from MRI images is crucial for diagnosing and assessing cardiovascular disease.However,the manual calculation of these parameters is challenging due to the high variability among patients and the time-consuming nature of the process.In this study,the authors introduce a framework named MultiJSQ,comprising the feature presentation network(FRN)and the indicator prediction network(IEN),which is designed for simultaneous joint segmentation and quantification.The FRN is tailored for representing global image features,facilitating the direct acquisition of left ventricle(LV)contour images through pixel classification.Additionally,the IEN incorporates specifically designed modules to extract relevant clinical indices.The authors’method considers the interdependence of different tasks,demonstrating the validity of these relationships and yielding favourable results.Through extensive experiments on cardiac MR images from 145 patients,MultiJSQ achieves impressive outcomes,with low mean absolute errors of 124 mm^(2),1.72 mm,and 1.21 mm for areas,dimensions,and regional wall thicknesses,respectively,along with a Dice metric score of 0.908.The experimental findings underscore the excellent performance of our framework in LV segmentation and quantification,highlighting its promising clinical application prospects.
基金supported by the funding from the National Natural Science Foundation of China(32072359)。
文摘Wheat powdery mildew caused by Blumeria graminis f.sp.tritici(Bgt)is an important disease worldwide.Detection of latent infection of leaves by the pathogen in late autumn is valuable for estimating the inoculum potential to assess disease risks in the spring.We developed a new tool for rapid detection and quantification of latent infection of seedlings by the pathogen.The method was based on recombinase polymerase amplification(RPA)coupled with an end-point detection via lateral flow device(LFD).The limit of detection is 100 agμL^(-1)of Bgt DNA,without noticeable interference from either other common wheat pathogens or wheat material(Triticum aestivum).It was evaluated on wheat seedlings for this accuracy and sensitivity in detecting latent infection of Bgt.We further extended this RPALFD assay to estimate the level of latent infection by Bgt based on imaging analysis.There was a strong correlation between the image-based and real-time PCR assay estimates of Bgt DNA.The present results suggested that this new tool can provide rapid and accurate quantification of Bgt in latently infected leaves and can be further development as an on-site monitoring tool.
文摘Abstract： In the present study, we established an ultra performance liquid chromatography coupled with time-of-flight mass spectrometry （UPLC-QTOF-MSE） method to simultaneously quantify 33 components in Ginkgo biloba leaf extracts （GBEs）, including 17 flavonol glycosides, five terpene trilactones （TTLs）, four polyphenols and seven carboxylic acids. This optimized method was successfully applied to analyze the explicit compositions of GBE samples collected from different places. Furthermore, the data were processed through unsupervised principal component analysis （PCA） and supervised orthogonal partial least squared discrimination analysis （OPLS-DA） to evaluate the quality and compare the differences between the samples according to the contents of the 33 chemical constituents. Bilobalide, protocatechuic acid, shikimic acid, quinic acid, ginkgolide B, ginkgolide J, kaempferol-3-O-rutinoside, isorhamnetin-3-O-rutinoside, quercetin-3-O-ct-L-rhamnopyranocyl-2＂-（6＇＂-p-coumaroyl）-β-D-glucoside and rutin were recognized as characteristic chemical markers that contributed most to control the quality of GBEs. Based on the fact that GBEs should be standardized with the characteristic components as quality control chemical markers, it is most important to maintain the quality of GBEs stable and reliable, and this method also provided a good strategy to further rectify and standardize the GBEs market.
基金National Natural Science Foundation of China(Grant No.30873416)
文摘In the present study, we developed and validated a high-performance liquid chromatography method for the simultaneous determination of seven phenylpropanoid compounds （2-hydroxyl cinnamaldehyde, coumarin, cinnamyl alcohol, cinnamic acid, 2-methoxy cinnamic acid, cinnamaldehyde and 2-methoxy cinnamaldehyde） in Cinnamomi Cortex and Cinnamomi Ramulus. The levels of seven phenylpropanoid compounds in Cinnamomi Cortex and Cinnamomi Ramulus were compared using this method. A total of 48 samples （27 Cinnamomi Cortex and 21 Cinnamomi Ramulus） were purchased in China and analyzed. Quantities of seven phenylpropanoid compounds ranged from 17.5 to 61.6 mg/g in Cinnamomi Cortex and ranged from 9.91 to 23.4 mg/g in Ciunamomi Ramulus. The level of 2-methoxy cinnamic acid in the Cinnamomi Cortex samples was below the LOD, whereas it ranged from 0 to 0.119 mg/g in the Cinnamomi Ramulus samples. The （cinnamyl alcohol＋cinnamic acid）/cinnamaldehyde ratios （R346） of Ciunamomi Cortex and Cinnamomi Ramulus ranged from 0.0121 to 0.0467 and 0.0598 to 0.182, respectively. This ratio could be used to discriminate Cinnamomi Cortex （〈0.05） and Cinnamomi Ramulus （〉0.05）. The extraction rates （Dn） of seven compounds in boiling water were different, with the lowest dissolution for cinnamaldehyde （〈3%） and the highest for cinnamic acid （about 60%）.
基金973 Project-Medical Characteristics of Orthodox Herbs(Grant No.2006CB504707)
文摘For the first time, we have utilized high-performance liquid chromatography （HPLC） to simultaneously quantify the eugenol and bancroffione in Caryophylli Fructus. The optimized parameters included： Inertsil ODS-4 column （150 mm×4.6 mm, 5 μm）; column temperature： 35 ℃; mobile phase： methanol water （65：35, v/v）; flow rate： 1.0 mL/min; detection wavelength： 280 nm. Eugenol and bancroftione showed good linear relationships with peak areas within the range of （0.0998 0.8982） mg/mL （r = 0.9999） and （0.1474-1.3266） mg/mL （r = 0.9999）, respectively. The average recoveries were 102.52% and 100.96% for eugenol and bancroftione, respectively. Our results showed that the established method is simple, rapid, and accurate with good reproducibility to evaluate the quality of Caryophylli Fructus.
文摘High-performance liquid chromatography with UV detection was used for the quantification of the flavonoid quercetin, the active compound found in ＂Guangdong Wang-bu-liu-xing＂. The method was developed and demonstrated to provide superior performance over other commonly documented methods. This HPLC assay achieved high specificity through the use of a reversed-phase C18 column eluted with a mobile phase of methanol-0.4% （v/v） phosphoric acid （50：50, v/v） over the course of 30 min. UV detection at 360 nm was used. This analytical method provided excellent precision and a mean recovery of 99%, demonstrated by repeated analysis of 11 sample groups. Because of its high performance and simplicity, this HPLC assay can be readily utilized as a practical method for the quality control of active compounds extracted from Guangdong Wang-bu-liu-xing.
文摘Real-time PCR is a closed DNA amplification system that skillfully integrates biochemical, photoelectric and computer techniques. Fluorescence data acquired once per cycle provides rapid absolute quantification of initial template copy numbers as PCR products are generated. This technique significantly simplifies and accelerates the process of producing reproducible quantification of nucleic acid molecules. It not only is a sensitive, accurate and rapid quantitative method, but it also provides an easier way to calculate the absolute starting copy number of nucleic acid molecules to be tested. Together with molecular bio-techniques, like microarray, real-time PCR will play a very important role in many aspects of molecular life science such as functional gene analysis and disease molecular diagnostics. This review introduces the detailed principles and application of the real-time PCR technique, describes a recently developed system for exact quantification of AUX/IAA genes In Arabidopsis, and discusses the problems with the real-time PCR process.
基金supported by Science Challenge Project of China (No. TZ2018007)National Natural Science Foundation of China (Nos. 11872088 and51975546)。
文摘This paper investigates the influence of crack orientation on damage quantification using Lamb wave in plate structures. Finite element simulation is performed to acquire Lamb wave signal responses for different configurations of crack orientations and crack lengths. Two Lamb wave features, namely the normalized amplitude and the phase change, are used as damage sensitive features to develop a crack size quantification model. A hypothesis based on the geometrical influence on signal features is proposed, and the crack size quantification model incorporating the orientation angle is established using the hypothesis. An index of Probability of Reliable Quantification(PRQ) is proposed to evaluate the performance of the model. The index can be used to determine the sizing risk in terms of probabilities. A realistic aluminum plate is used to obtain the experimental data using piezoelectric(PZT) wafer-type sensors around a center through crack. The experimental data are used to validate the overall method. Results indicate that the proposed model can yield reliable results for size quantification of inclined cracks.
文摘AIM To investigate and compare the analytical and clinical performance of Tian Long automatic hypersensitive hepatitis B virus(HBV) DNA quantification system and Roche CAP/CTM system.METHODS Two hundred blood samples for HBV DNA testing, HBV-DNA negative samples and high-titer HBV-DNA mixture samples were collected and prepared. National standard materials for serum HBV and a worldwide HBV DNA panel were employed for performance verification. The analytical performance, such as limit of detection, limit of quantification, accuracy, precision, reproducibility, linearity, genotype coverage and cross-contamination, was determined using the Tian Long automatic hypersensitive HBV DNA quantification system(TL system). Correlation and Bland-Altman plot analyses were carried out to compare the clinical performance of the TL system assay and the CAP/CTM system. RESULTS The detection limit of the TL system was 10 IU/m L, and its limit of quantification was 30 IU/m L. The differences between the expected and tested concentrations of the national standards were less than ± 0.4 Log10 IU/m L, which showed high accuracy of the system. Results of the precision, reproducibility and linearity tests showed that the multiple test coefficient of variation(CV) of the same sample was less than 5% for 102-106 IU/m L; and for 30-108 IU/m L, the linear correlation coefficient r2 = 0.99. The TL system detected HBV DNA(A-H) genotypes and there was no cross-contamination during the "checkerboard" test. When compared with the CAP/CTM assay, the two assays showed 100% consistency in both negative and positive sample results(15 negative samples and 185 positive samples). No statistical differences between the two assays in the HBV DNA quantification values were observed(P > 0.05). Correlation analysis indicated a significant correlation between the two assays, r2 = 0.9774. The Bland-Altman plot analysis showed that 98.9% of the positive data were within the 95% acceptable range, and the maximum difference was-0.49.CONCLUSION The TL system has good analytical performance, and exhibits good agreement with the CAP/CTM system in clinical performance.
基金National Key Basic Research Program of China,No.2010CB428403National Grand Science and Technology Special Project of Water Pollution Control and Improvement,No.2009ZX07210-006
文摘The regional hydrological system is extremely complex because it is affected not only by physical factors but also by human dimensions.And the hydrological models play a very important role in simulating the complex system.However,there have not been effective methods for the model reliability and uncertainty analysis due to its complexity and difficulty.The uncertainties in hydrological modeling come from four important aspects：uncertainties in input data and parameters,uncertainties in model structure,uncertainties in analysis method and the initial and boundary conditions.This paper systematically reviewed the recent advances in the study of the uncertainty analysis approaches in the large-scale complex hydrological model on the basis of uncertainty sources.Also,the shortcomings and insufficiencies in the uncertainty analysis for complex hydrological models are pointed out.And then a new uncertainty quantification platform PSUADE and its uncertainty quantification methods were introduced,which will be a powerful tool and platform for uncertainty analysis of large-scale complex hydrological models.Finally,some future perspectives on uncertainty quantification are put forward.
基金supported by National Natural Science Foundation of China(No.81360474)
文摘The present study was designed to investigate the bioactive constituents ofXanthoceras sorbifolia in terms of amounts and their antioxidant, DNA scissiou protection, and ct-glucosidase inhibitory activities. Simultaneous quantification of 10 X. sorbifolia constituents was carried out by a newly established ultra-high performance liquid chromatography-quadrupole mass spectrometry method （UHPLC-MS）, The antioxidant activities were evaluated by measuring DPPH radical scavenging and DNA scission protective activities. The a-glucosidase inhibitory activities were investigated by using an assay with a-glucosidase from Bacillus Stearothermophilus and disaccharidases from mouse intestine. We found that the wood ofX. sorbifolia was rich in phenolic compounds with the contents of catechin, epicatechin, myricetin, and dihydromyricetin being 0.12-0.19, 1.94-2.16, 0.77-0.91, and 6.76-7.89 mg.g-1, respectively. The four constituents strongly scavenged DPPH radicals （with ECs0 being 4.2, 3.8 and 5.7 μg-mL-1, respectively） and remarkably protected peroxyl radical-induced DNA strand scission （92.10%, 94.66%, 75.44% and 89.95% of protection, respectively, at a concentration of 10 μmol.L-1）. A dimeric flavan 3-ol, epigallocatechin-（4β→8, 2β→O-7）-epicatechin potently inhibited a-glucosidase with an IC50 value being as low as 1.2 μg.mL1. The established UHPLC-MS method could serve as a quality control tool for X. sorbifolia. In conclusion, the high contents of antioxidant and α-glucosidase inhibitory constituents in X. sorbifolia support its use as complementation of other therapeutic agents for metabolic disorders, such as diabetes and hypertension.
基金funded by the National Natural Science Foundation of China(No.52006177)National Science and Technology Major Project,China(No.2017-II-0009-0023)。
文摘Manufactured blades are inevitably different from their design intent,which leads to a deviation of the performance from the intended value.To quantify the associated performance uncertainty,many approaches have been developed.The traditional Monte Carlo method based on a Computational Fluid Dynamics solver(MC-CFD)for a three-dimensional compressor is prohibitively expensive.Existing alternatives to the MC-CFD,such as surrogate models and secondorder derivatives based on the adjoint method,can greatly reduce the computational cost.Nevertheless,they will encounter’the curse of dimensionality’except for the linear model based on the adjoint gradient(called MC-adj-linear).However,the MC-adj-linear model neglects the nonlinearity of the performance function.In this work,an improved method is proposed to circumvent the lowaccuracy problem of the MC-adj-linear without incurring the high cost of other alternative models.The method is applied to the study of the aerodynamic performance of an annular transonic compressor cascade,subject to prescribed geometric variability with industrial relevance.It is found that the proposed method achieves a significant accuracy improvement over the MC-adj-linear with low computational cost,showing the great potential for fast uncertainty quantification.
基金the financial support of the Science and Technology Innovation Program of Hunan Province(2020RC5003)the research and application of key technologies for zero-energy buildings based on distributed energy storage and air conditioning demand response(2020-K-165)+1 种基金the Technology Innovation Program of Hunan Province(2017XK2015)the Technology Innovation Program of Hunan Province(2020RC2017)。
文摘This paper reviews recent research on the demand flexibility of residential buildings in regard to definitions,flexible loads,and quantification methods.A systematic distinction of the terminology is made,including the demand flexibility,operation flexibility,and energy flexibility of buildings.A comprehensive definition of building demand flexibility is proposed based on an analysis of the existing definitions.Moreover,the flexibility capabilities and operation characteristics of the main residential flexible loads are summarized and compared.Models and evaluation indicators to quantify the flexibility of these flexible loads are reviewed and summarized.Current research gaps and challenges are identified and analyzed as well.The results indicate that previous studies have focused on the flexibility of central air conditioning,electric water heaters,wet appliances,refrigerators,and lighting,where the proportion of studies focusing on each of these subjects is 36.7%,25.7%,14.7%,9.2%,and 8.3%,respectively.These flexible loads are different in running modes,usage frequencies,seasons,and capabilities for shedding,shifting,and modulation,while their response characteristics are not yet clear.Furthermore,recommendations are given for the application of white-,black-,and grey-box models for modeling flexible loads in different situations.Numerous static flexibility evaluation indicators that are based on the aspects of power,temporality,energy,efficiency,economics,and the environment have been proposed in previous publications,but a consensus and standardized evaluation framework is lacking.This review can help readers better understand building demand flexibility and learn about the characteristics of different residential flexible loads,while also providing suggestions for future research on the modeling techniques and evaluation metrics of residential building demand flexibility.
