Objective: The importance of culture in nursing lies in its influence on patients’ health perceptions, behaviors, and responses to care. Despite the challenges posed by the complexity of studying culture, there remai...Objective: The importance of culture in nursing lies in its influence on patients’ health perceptions, behaviors, and responses to care. Despite the challenges posed by the complexity of studying culture, there remains a critical need to develop cultural competence in nursing. This study investigated how nursing students in rural Indonesia enhance their cultural competence through education and practice. Methods: This study employed a qualitative descriptive phenomenological approach. It conducted face-to-face interviews with 32 students across 6 regions, selected purposively. Thematic analysis was employed to evaluate the results. Results: Five themes emerge in this research: perceptions of cultural competence, learning cultural competence in the classroom, practical experience, learning expectations related to cultural competence, and cultural learning barriers. Conclusions: Enhancing cultural competence necessitates enriched curricula, adept clinical mentors, and continual student evaluation. This iterative assessment informs tailored strategies for nurturing cultural proficiency.展开更多
Objectives To investigate the barriers and facilitators influencing the sustainable implementation of evidence-based practice(EBP)for Peristomal Irritant Contact Dermatitis(PICD)based on the Consolidated Framework for...Objectives To investigate the barriers and facilitators influencing the sustainable implementation of evidence-based practice(EBP)for Peristomal Irritant Contact Dermatitis(PICD)based on the Consolidated Framework for Implementation Research(CFIR).Methods The sample consisted of 17 nurses from three urology wards at a tertiary hospital in Beijing,China,who had participated in the EBP.Guided by the CFIR,we identified constructs influencing the sustainability of implementation by conducting a directed content analysis of the 17 individual in-depth interviews.By rating,the valence and magnitude of each construct were determined.Results This study identified 19 factors across the five domains of the CFIR.Among these determinants,16 were identified as barriers,while three were recognized as facilitators.Common barriers across different contexts were identified,such as delayed evidence updating,the complexity of intervention components and steps,and incompatibility between the implementation process and clinical practices.In contrast,facilitators of intervention implementation were regular communication and feedback,support and drive from principals and a positive cultural atmosphere.In addition,we identified“regular evidence updating”as an independent construct outside of the CFIR,a finding that will provide key information for updating the CFIR framework.Conclusions Regular evidence updating is crucial for the sustainability of EBP implementation.Researchers should receive consistent feedback from practitioners and adjust or modify the EBP as necessary.Additionally,researchers must consider the compatibility or adaptability of the EBP’s content with clinical practice in formulating it rather than adding additional complexity to the original workflow.展开更多
Objective:This study aimed to summarize the quality of life experiences of individuals with physical frailty undergoing hemodialysis or peritoneal dialysis and to identify the difficultiesand resources that enable bet...Objective:This study aimed to summarize the quality of life experiences of individuals with physical frailty undergoing hemodialysis or peritoneal dialysis and to identify the difficultiesand resources that enable better person-centered health care.Methods:The study described is a qualitative meta-synthesis literature review.The search was performed in databases such as CINAHL,Scopus,PubMed,Web of Science,the Cochrane Library,and Cuiden Plus through Medical Subject Headings and free terms.Qualitative and mixed studies were included on individuals undergoing hemodialysis or peritoneal dialysis,18 years of age or older,published in English or Spanish,between January 2013 and January 2024.The Mixed Methods Appraisal Tool was used to assess the methodological quality.The information was analyzed and coded through a socioecological model and the social determinants of health.Results:Fourteen qualitative and two mixed articles were selected.A total of 256 individuals participated in the study.Seventeen themes and 25 sub-themes were identifiedand grouped into two blocks(difficulties and resources).The difficultiesthat stood out were a low tolerance for activities of daily living and physical activity,loss of self-control over life and social roles,and lack of community and public resources.As for the resources,the following was found:the positive meaning of dialysis,the safety offered by close individuals and the healthcare team,the activation of specificprograms,and person-centered policies.Conclusions:The analysis and interpretation of the identifieddifficultiesand resources revealed key elements to consider when designing and implementing health programs for individuals undergoing dialysis.Future research should explore these dimensions in diverse cultural and geographical contexts to enhance generalizability and support health equity.展开更多
Objectives Nurses’clinical research activities have contributed to optimizing the care process and improving patient outcomes,and generative artificial intelligence(GAI)may help clinical nurses strengthen their resea...Objectives Nurses’clinical research activities have contributed to optimizing the care process and improving patient outcomes,and generative artificial intelligence(GAI)may help clinical nurses strengthen their research skills.To support research,this study aimed to explore the Chinese nurses’perceptions and experiences of GAI training.Methods This study used a descriptive qualitative design.The China Nurses Network conducted a three-day training session on“GAI for Nursing Research”theme,we selected 23 nurses by a convenience sampling method among participating in the training.The researchers conducted three focus group interviews at the end of each day.All focus groups were interviewed face-to-face to facilitate interaction,data collection,and observation.The data were analyzed using conventional content analysis and coded manually.Results The results showed that nurses’use of GAI to support scientific research was dynamic and characterized by evolving perceptions and practices.Four themes and 11 sub-themes emerged from the analysis:1)utilization efficacy:cope with research ability,affected by many factors;2)booster research:growth and challenges go hand in hand;3)role reversal:from GAI-dominated to nurse-dominated;4)beautiful dream:more features on research,more assistants on clinical care.Conclusions The effectiveness of GAI in supporting clinical nurses in conducting research is mainly limited by differences in personal research literacy,lack of ethical regulation,and information accuracy.In the future,it is necessary to improve nurses’relevant skills through specialized training and promote the standardization of technical regulations to ensure the appropriate application of GAI in nursing research.展开更多
BACKGROUND Older patients often experience thirst after general anesthesia for gastrointestinal surgery,particularly during the post-anesthesia care unit(PACU)phase.Thirst not only compromises patient comfort but also...BACKGROUND Older patients often experience thirst after general anesthesia for gastrointestinal surgery,particularly during the post-anesthesia care unit(PACU)phase.Thirst not only compromises patient comfort but also induces anxiety,which may hinder recovery.Although previous studies have addressed postoperative thirst,research specifically examining thirst experiences and nursing needs in older patients remains limited.AIM To explore thirst experiences and nursing needs of older PACU patients following gastrointestinal surgery,aiming to inform targeted interventions.METHODS This study employed a phenomenological approach within a qualitative research framework.A purposeful sampling method was used to select 12 older patients who underwent gastrointestinal surgery under general anesthesia at a tertiary hospital in Shanghai between November and December 2024.Data were collected through semi-structured interviews and analyzed using Colaizzi’s seven-step method.Themes were extracted from the interview data.RESULTS Analysis of the interview data identified four main themes and eight subthemes:(1)Intense sensations of thirst post-surgery(subjective experience of thirst and duration of thirst);(2)Emotional experiences of thirst-related discomfort(anxiety,irritability,and helplessness);(3)Practical challenges in relieving thirst(limitations of current interventions and nursing response time);and(4)Patient expectations of nursing care(desire for more timely interventions and expectation for more proactive attention from nursing staff).CONCLUSION Older patients frequently experience substantial thirst discomfort after gastrointestinal surgery under general anesthesia.A multidisciplinary perioperative intervention plan is essential to alleviate these symptoms and enhance postoperative comfort.展开更多
Objectives:At the time of the bombing of the federal building in Oklahoma City,Oklahoma(OKC),it was the deadliest terrorist attack in the United States of America.Available research on this incident,and in general,has...Objectives:At the time of the bombing of the federal building in Oklahoma City,Oklahoma(OKC),it was the deadliest terrorist attack in the United States of America.Available research on this incident,and in general,has been quantitative,using deductive methods.The purpose of the current study was to systematically examine professional disaster response workers’emotions elicited spontaneously and in detail as they were experienced over time after a major disaster.This qualitative study will add to existing knowledge of psychopathology and the psychosocial effects of disasters on professional responders,which have not been explored by prior quantitative studies.Methods:A volunteer sample of 181 rescue and recovery workers for the 1995 bombing of the OKC Murrah Federal Building was interviewed approximately 3 years after the bombing.These responders were asked open-ended questions to elicit undirected responses in their own words.In these interviews,they were prompted to describe their feelings at 3 distinct time points:upon their arrival at the bombing scene(“immediately”),in the first 1 week following the bombing,and currently at the time of the interview(“now”).Results:Data items were coded into the 5 themes:Anticipation,Shock and awe,Negative reactions,Positive reactions,and Emotionally unengaged/unaffected.The emotional themes of the responders mapped into 2 conceptual domains.The first domain is related to common human responses to horrible events such as the bombing.The second domain is related to the professional identities of the disaster responders.Conclusions:Specific aspects of the professional responder roles asserted themselves,shaping their emotional responses distinctly from those of the bomb blast survivors.This study identified both negative and positive emotions,which may help identify risk for or resistance to posttraumatic stress disorder(PTSD).展开更多
Objective:This study aimed to investigate the learning expectations of undergraduate nursing students regarding the flipped Health Assessment course.Methods:This descriptive,qualitative study was conducted at a medica...Objective:This study aimed to investigate the learning expectations of undergraduate nursing students regarding the flipped Health Assessment course.Methods:This descriptive,qualitative study was conducted at a medical university in Fuzhou,Fujian Province,China.An interview outline was designed based on the core dimensions of Expectation Confirmation Theory(expectation sources,expectation content,and expectation importance).Thirty second-year undergraduate nursing students who had completed first-year basic medical courses and were about to take the flipped Health Assessment course were interviewed between June and July 2022.Interview data were analyzed using qualitative content analysis.Results:Five major themes and thirteen subthemes were identified.Theme 1 was expectation sourcesperceived learning difficulties from past experiences,which included four subthemes:insufficient autonomous learning ability,confusion regarding learning methods,insufficient engagement in learning,and low professional identity.Theme 2 included knowledge and information expectations,which comprised three subthemes:knowledge to improve professional competence,knowledge to enhance academic competitiveness,and knowledge to boost self-efficacy.Theme 3 comprised logical expectations and included two subthemes:flexible teaching methods and efficient instructional tools.Theme 4,pleasure expectations,included two subthemes:vivid teaching styles and diversified teaching evaluations.Theme 5 comprised professional value expectations and included two subthemes:teachers'responsible professional attitudes and gentle emotional support.Conclusion:Students'learning expectations originate from perceived learning difficulties,such as insufficient learning autonomy and engagement,confusion about learning methods,and a lack of professional identity.They showed various learning expectations for the flipped Health Assessment course,including knowledge and information,logic,pleasure,and professional value expectations.展开更多
Objective:This study aims to explore the experiences of social alienation among adolescents with depression,providing practical This study aims to explore the experiences of social alienation among adolescents with de...Objective:This study aims to explore the experiences of social alienation among adolescents with depression,providing practical This study aims to explore the experiences of social alienation among adolescents with depression,providing practical guidance for improving their interpersonal relationships and facilitating their reintegration into society.Methods:This qualitative research was conducted following the conventional content analysis method.20 adolescents with depression were employed to select from June to August 2024 for face-to-face semi-structured interviews.The collected data were analyzed using Colaizzi's seven-step method.Results:Three themes and eight sub-themes were analyzed and identified:individual level(feelings of helplessness and powerlessness,cognitive distortion,avoidance and withdrawal),family level(lack of family awareness,family conflict),social level(limitations of academic stress and social circle,lack and degradation of skills,generalization of virtual reality,social“stigma”).Conclusion:Adolescents with depression experience complex social alienation.Healthcare providers should enhance their self-awareness and social adaptation skills,improve family dynamics,and provide a comprehensive range of services and services to help them to cope with the challenges of depression.Healthcare providers should enhance their self-awareness and social adaptation skills,improve family dynamics,strengthen communication,bolster family support systems,and collaborate to develop comprehensive social networks and psychological services.This will create a supportive social atmosphere to help adolescents gradually alleviate their feelings of social alienation.展开更多
Objective:To explain barriers to childbearing among working women from the perspective of working women and midwives.Methods:In this study,a qualitative study of content analysis method was employed.The study was cond...Objective:To explain barriers to childbearing among working women from the perspective of working women and midwives.Methods:In this study,a qualitative study of content analysis method was employed.The study was conducted from December 2021 to July 2022.The population consisted of working women and midwives.Data collection was conducted using semi-structured interviews and continued until data saturation was achieved.Through purposeful sampling,10 women and 11 midwives were selected as the sample.The data were analyzed by Graneheim and Lundman content analysis approach.For more rigorous,Nvivo 14 software was also used for data management,analysis,coding and organization of data.Results:This study included 21 women(10 women employees and 11 midwives)aged 25-43 years(for women employees)and 27-56 years(for midwives)with 1 to 34 years of job experience.In examination of participant’views about the barriers to childbearing,1124 codes were identified,of which we managed to extract six subthemes and two themes.Two themes emerged:“lack of social support and lack of family support”.Subthemes of“lack of social support”were“inadequate leave,long distance from workplace,and insufficient wages”.Subthemes of“lack of family support”were“lack of spouse support,lack of spouse's family support,and lack of their family support”.Conclusions:Different socioeconomic factors influence childbearing tendency in working women.Social and family supports play an important role in this regard.It is important to take these factors into account at the time of policymaking in the field of midwifery.展开更多
Objective:To gain insight into the potential factors that may cause perceived stress and anxiety in simulation education.Methods:A secondary qualitative analysis study using qualitative thematic synthesis.A local high...Objective:To gain insight into the potential factors that may cause perceived stress and anxiety in simulation education.Methods:A secondary qualitative analysis study using qualitative thematic synthesis.A local higher education institution that conducted pre-registration nursing programs.A total of 189 undergraduate nursing students that were never attained any clinical placement prior to the parent study.Focus group interviews were conducted to collect data that were then transcribed and analyzed through the qualitative thematic synthesis approach to develop themes.Results:Three themes were emerged from the participants’simulation experiences in terms of peoples(the observers vs the observed),actions(the prepared vs the unprepared),and settings(the realism vs the simulation).Conclusions:By considering and reviewing the current design and development of the simulation practice,the findings of this study contribute to the body of knowledge with valuable insights on stress and anxiety that may affect students’learning in simulation.展开更多
Objective:Patient safety culture is a concern in every healthcare organization,therefore,the healthcare leadership is encountering issues related to patient safety across the globe.In India,there is limited research a...Objective:Patient safety culture is a concern in every healthcare organization,therefore,the healthcare leadership is encountering issues related to patient safety across the globe.In India,there is limited research and information about patient safety culture among healthcare stakeholders and there is relatively little qualitative research available that captures the factors of patient safety culture.Hence,this study aims to explore the perception of healthcare professionals on patient safety culture.Methods:An exploratory qualitative study design was adopted in a tertiary care hospital.Structured focus group discussion(FGD)(n=4)among healthcare professionals and two in-depth interview focus groups were audio-recorded and transcribed.Two coders reviewed transcripts using the editing approach and organized codes into themes.The data were analyzed through MAXQDA 2022(VERBI Software GmbH,Berlin,Germany),qualitative data analysis software,and descriptive analysis technique.The main codes and themes were generated using inductive and deductive method and smart coding was done.Results:Overall,there were 190 unique mentions of codes related to patient safety culture from 4 FGDs.They were categorized into 6 major themes and subcodes were derived via smart coding using the MAXQDA software.“Resources and constraints”was the most prominent code,followed by management support,manpower shortage,burnout,and lack of personnel commitment.Conclusions:The study highlights significant gaps in patient safety culture within the healthcare setting,with resource constraints,management support,and manpower shortages emerging as critical challenges.Burnout and lack of personnel commitment further exacerbate these issues,underscoring the need for targeted interventions.展开更多
Infrared(IR)spectroscopy,a technique within the realm of molecular vibrational spectroscopy,furnishes distinctive chemical signatures pivotal for both structural analysis and compound identification.A notable challeng...Infrared(IR)spectroscopy,a technique within the realm of molecular vibrational spectroscopy,furnishes distinctive chemical signatures pivotal for both structural analysis and compound identification.A notable challenge emerges from the misalignment between the mid-IR light wavelength range and molecular dimensions,culminating in a constrained absorption cross-section and diminished vibrational absorption coefficients(Supplementary data).展开更多
Objective:To explore the perspectives of high-risk pregnant women regarding the home care program for high-risk pregnancies.Methods:This qualitative study was conducted in a university hospital and 5 comprehensive hea...Objective:To explore the perspectives of high-risk pregnant women regarding the home care program for high-risk pregnancies.Methods:This qualitative study was conducted in a university hospital and 5 comprehensive health centers in Ahvaz,Iran,from February 2023 to July 2023.The sampling method used was purposive sampling considering the maximum possible diversity,which continued until data saturation.11 in-depth and semistructured interviews were conducted with high-risk pregnant women.Data were simultaneously analysed using Graneheim and Lundman content analysis by MAXQDA version 20 software.Results:Data analysis extracted 3 themes,12 categories,and 26 subcategories.The themes and categories were“high-risk pregnancy and related issues(pursuing treatment and paying attention to the high-risk situation by pregnant women,psychological aspect of high-risk pregnancy on pregnant women and caregivers,emotional and physical aspects of high-risk pregnancy,the consequences of high-risk pregnancy on the people around them and empowerment of high-risk pregnant women)”,“support dimension(family and community support for high-risk pregnant women)”,and“framework of program(acculturalization,means and equipment,security,manpower and the necessary arrangements for the home care)”.Conclusions:High-risk pregnancy affects various aspects of the lives of high-risk pregnant women and their families.Creating the necessary conditions for providing care at home plays a vital role in supporting high-risk pregnant women and removing related obstacles to receiving prenatal care in person.It will be especially beneficial for high-risk pregnant women in low-income areas.展开更多
Background and Objectives:Non-Communicable Diseases(NCDs)represent a significant public health burden in China,particularly in cold regions where environmental factors further limit access to healthcare.To address thi...Background and Objectives:Non-Communicable Diseases(NCDs)represent a significant public health burden in China,particularly in cold regions where environmental factors further limit access to healthcare.To address this challenge,China has implemented the National Essential Public Health Service Package(NEPHSP)to improve NCD management through primary healthcare services.However,research on the effectiveness of NEPHSP in cold regions is limited.This study aims to identify the key factors influencing the implementation of NCD management services in cold regions,focusing on both barriers and facilitators.Methods:This qualitative study was conducted in three purposively selected cold regions in Northeast China(Harbin,Qiqihar,and Jiamusi).Data were collected through semi-structured interviews and focus group discussions,guided by the RE-AIM framework.Participants included local health administrators,primary healthcare workers,and community-dwelling individuals with NCDs.Thematic analysis was employed to code and extract key themes from the data.Results:A total of 72 participants(59.7%female,mean age 49.1±6.24 years)took part in the study.Major barriers to NCD management included low health literacy,limited awareness of NCDs,insufficient medical resources,poor public awareness,inadequate multisectoral collaboration,and the absence of effective audit and feedback systems.Key facilitators included the affordability and accessibility of primary healthcare services,strong patient-physician relationships,and the high priority given to NCD management by local governments.Furthermore,harsh environmental conditions and limited healthcare accessibility in cold regions complicated the management of NCDs,highlighting the need for tailored interventions that address the specific challenges of these regions.Conclusion:This study identifies significant facilitators and barriers to the implementation of NCD management services under the NEPHSP in cold regions of China.The unique challenges posed by cold climates necessitate the adaptation of healthcare services to local needs,with a focus on improving health literacy,enhancing healthcare infrastructure,and fostering multisectoral collaboration.These findings provide valuable insights for optimizing NCD prevention and management strategies within China's primary healthcare system.展开更多
Objective:Through interviews with oral specialist nurses,to explore the influencing factors of training transfer for oral specialist nurses,and provide references for scientifically improving the effect of specialist ...Objective:Through interviews with oral specialist nurses,to explore the influencing factors of training transfer for oral specialist nurses,and provide references for scientifically improving the effect of specialist nurse training transfer.Methods:Using purposive sampling and descriptive qualitative research,15 oral specialist nurses from two tertiary hospitals in Guangdong Province were selected for semi-structured interviews,and data analysis was performed.Results:After collating and analyzing the interview data,three types of factors affecting the training transfer of oral specialist nurses were proposed,including personal characteristics,training management,and training transfer atmosphere.Conclusion:Managers should formulate a comprehensive and systematic plan based on the influencing factors of oral specialist nurse training transfer to effectively promote training transfer behavior and enhance training transfer effects.展开更多
Huang-Lian-Jie-Du-Decoction （HLJDD） has been widely used for the treatment of Alzheimer＇s disease （AD） in clinic. However, the relationship between its chemical profile and neuroprotective bioactivity was not cle...Huang-Lian-Jie-Du-Decoction （HLJDD） has been widely used for the treatment of Alzheimer＇s disease （AD） in clinic. However, the relationship between its chemical profile and neuroprotective bioactivity was not clearly clarified yet. In present study, the water extract of HLJDD and subsequent three polarity fractions divided by different reagents were investigated. A total of 17 chromatographic peaks were confirmed by comparison with standards and their UV, MS spectra. Among them, 11 major compounds were determined by HPLC-DAD method with good linear regression relationship （r2, 0.9994-0.9999）, precisions （inter-day precision RSD, 0.79%-1.07%; intra-day precision RSD, 1.59%-2.10%）, repeatability （RSD, 1.66%-3.67%）, stability （RSD, 1.26%-4.77%） and recovery （95.24%-105.41%, RSD, 0.29%-2.69%）. Furthermore, PC12 cells and primary neurons cells were used for the neuroprotective effective assessment of aforementioned four samples from HLJDD. 3＂he total aqueous extract and n-butanol extract of HLJDD presented more significant effects than the other two parts. According to their quality and quantity determination results, iridoids and alkaloids have a positive correlation with the neuroprotective effectiveness of HLJDD.展开更多
[Objective] The aim was to establish an event-specific qualitative PCR method for transgenic soybean MON89788.[Method] Firstly,the 3′-junction sequence between host plant DNA and integrated DNA of transgenic MON89788...[Objective] The aim was to establish an event-specific qualitative PCR method for transgenic soybean MON89788.[Method] Firstly,the 3′-junction sequence between host plant DNA and integrated DNA of transgenic MON89788 soybean was isolated using thermal asymmetric interlaced-PCR （TAIL-PCR）,and the specific PCR primers were designed based on the 3′-junction sequence.Secondly,the specificity and sensitivity of the qualitative PCR detection methods employing these primers were tested.[Result] 1 142-bp 3′-junction sequence was obtained.According to the sequence,event-specific qualitative PCR method was established,amplifying a 170-bp product specifically from MON89788 event,and the limit of detection was 0.05%,approximately 40 initial template copies.[Conclusion] The method was highly specific,sensitive,and suitable for detection of MON89788 event.展开更多
Given the great strides that China＇s education sector has made in recent decades, it can be expected that the overall workforce quality of exiting China＇s labor market in coming one or two decades will be relatively...Given the great strides that China＇s education sector has made in recent decades, it can be expected that the overall workforce quality of exiting China＇s labor market in coming one or two decades will be relatively low, while the overall quality of workforce newly entering into China＇s labor market will be high. As the new, bettereducated generation takes over, China＇s workforce quality will be vastly improved. This in turn will promote economic growth. We refer to economic growth arising from improved workforce quality as qualitative demographic dividend. Using the computable general equilibrium （CGE） model, this paper investigates the relationship between workforce quality improvements and economic growth. According to the model＇s results, an improvement in workforce quality will raise the economic growth rate by about two percentage points per annum between 2016 and 2020 and by 10 percentage points cumulatively by 2020. In other words, GDP will be 1.1 times the level of baseline GDP by 2020 due to the improved education levels. Given different production functions across sectors, the improvement of workforce quality will affect different sectors in different ways. On the whole, the improvement of workforce quality is more favorable to the development of capital-intensive sectors and sectors with rapid technology progress. According to this paper, considering the improvement of workforce quality, we cannot conclude that China＇s potential economic growth rate has already begun to decline. Despite diminishing conventional quantitative demographic dividends, China ＂s qualitative demographic dividends will keep rising. Qualitative demographic dividends will further push forward China＇s industrial restructuring and the strategic transition of industrial competitiveness from quantitative to qualitative and from an extensive to an intensive pattern of development.展开更多
The development of a core collection could enhance the utilization of germplasm collections in crop improvement programs and simplify their management. Selection of an appropriate sampling strategy is an important pre...The development of a core collection could enhance the utilization of germplasm collections in crop improvement programs and simplify their management. Selection of an appropriate sampling strategy is an important prerequisite to construct a core collection with appropriate size in order to adequately represent the genetic spectrum and maximally capture the genetic diversity in available crop collections. The present study was initiated to construct nested core collections to determine the appropriate sample size to represent the genetic diversity of rice landrace collection based on 15 quantitative traits and 34 qualitative traits of 2 262 rice accessions. The results showed that 50-225 nested core collections, whose sampling rate was 2.2%-9.9%, were sufficient to maintain the maximum genetic diversity of the initial collections. Of these, 150 accessions （6.6%） could capture the maximal genetic diversity of the initial collection. Three data types, i.e. qualitative traits （QT1）, quantitative traits （QT2） and integrated qualitative and quantitative traits （QTT）, were compared for their efficiency in constructing core collections based on the weighted pair-group average method combined with stepwise clustering and preferred sampling on adjusted Euclidean distances. Every combining scheme constructed eight rice core collections （225, 200, 175, 150, 125, 100, 75 and 50）. The results showed that the QTT data was the best in constructing a core collection as indicated by the genetic diversity of core collections. A core collection constructed only on the information of QT1 could not represent the initial collection effectively. QTT should be used together to construct a productive core collection.展开更多
A new approach to model and control an unknown system using subjective uncertain rules is proposed. This method is established by combining the grey system theory and the qualitative simulation method. The proposed ap...A new approach to model and control an unknown system using subjective uncertain rules is proposed. This method is established by combining the grey system theory and the qualitative simulation method. The proposed approach mainly contains three steps. In the first step, subjective uncertain rules are accumulated gradually during cognizing the system; the mapping relations between the system inputs and outputs are built and represented using the grey qualitative matrix in the second step; in the third step,the generalized whitening function is defined to realize the transformation between qualitative and quantitative information. Besides the theoretical results, two sets of simulations based on a water level control system are conducted comparatively to demonstrate the effectiveness of the proposed method. The water level expectation is set to be constant in the first set, while it changes in the second set. The simulation results show that the proposed method tracks the water level expectation well. By combining the proposed method with proportional-integral-derivative(PID) or fuzzy logic controller(FLC), it can be concluded that the system can reach the stable state more quickly and the overshoot can also be reduced compared to using PID or FLC alone.展开更多
基金supported by an internal grant from Universitas Citra Bangsa(Number 6/VI/LP3M/UCB/22).
文摘Objective: The importance of culture in nursing lies in its influence on patients’ health perceptions, behaviors, and responses to care. Despite the challenges posed by the complexity of studying culture, there remains a critical need to develop cultural competence in nursing. This study investigated how nursing students in rural Indonesia enhance their cultural competence through education and practice. Methods: This study employed a qualitative descriptive phenomenological approach. It conducted face-to-face interviews with 32 students across 6 regions, selected purposively. Thematic analysis was employed to evaluate the results. Results: Five themes emerge in this research: perceptions of cultural competence, learning cultural competence in the classroom, practical experience, learning expectations related to cultural competence, and cultural learning barriers. Conclusions: Enhancing cultural competence necessitates enriched curricula, adept clinical mentors, and continual student evaluation. This iterative assessment informs tailored strategies for nurturing cultural proficiency.
基金supported by the Peking University Nursing Discipline Research Development Fund(No.TYZH2023001)。
文摘Objectives To investigate the barriers and facilitators influencing the sustainable implementation of evidence-based practice(EBP)for Peristomal Irritant Contact Dermatitis(PICD)based on the Consolidated Framework for Implementation Research(CFIR).Methods The sample consisted of 17 nurses from three urology wards at a tertiary hospital in Beijing,China,who had participated in the EBP.Guided by the CFIR,we identified constructs influencing the sustainability of implementation by conducting a directed content analysis of the 17 individual in-depth interviews.By rating,the valence and magnitude of each construct were determined.Results This study identified 19 factors across the five domains of the CFIR.Among these determinants,16 were identified as barriers,while three were recognized as facilitators.Common barriers across different contexts were identified,such as delayed evidence updating,the complexity of intervention components and steps,and incompatibility between the implementation process and clinical practices.In contrast,facilitators of intervention implementation were regular communication and feedback,support and drive from principals and a positive cultural atmosphere.In addition,we identified“regular evidence updating”as an independent construct outside of the CFIR,a finding that will provide key information for updating the CFIR framework.Conclusions Regular evidence updating is crucial for the sustainability of EBP implementation.Researchers should receive consistent feedback from practitioners and adjust or modify the EBP as necessary.Additionally,researchers must consider the compatibility or adaptability of the EBP’s content with clinical practice in formulating it rather than adding additional complexity to the original workflow.
文摘Objective:This study aimed to summarize the quality of life experiences of individuals with physical frailty undergoing hemodialysis or peritoneal dialysis and to identify the difficultiesand resources that enable better person-centered health care.Methods:The study described is a qualitative meta-synthesis literature review.The search was performed in databases such as CINAHL,Scopus,PubMed,Web of Science,the Cochrane Library,and Cuiden Plus through Medical Subject Headings and free terms.Qualitative and mixed studies were included on individuals undergoing hemodialysis or peritoneal dialysis,18 years of age or older,published in English or Spanish,between January 2013 and January 2024.The Mixed Methods Appraisal Tool was used to assess the methodological quality.The information was analyzed and coded through a socioecological model and the social determinants of health.Results:Fourteen qualitative and two mixed articles were selected.A total of 256 individuals participated in the study.Seventeen themes and 25 sub-themes were identifiedand grouped into two blocks(difficulties and resources).The difficultiesthat stood out were a low tolerance for activities of daily living and physical activity,loss of self-control over life and social roles,and lack of community and public resources.As for the resources,the following was found:the positive meaning of dialysis,the safety offered by close individuals and the healthcare team,the activation of specificprograms,and person-centered policies.Conclusions:The analysis and interpretation of the identifieddifficultiesand resources revealed key elements to consider when designing and implementing health programs for individuals undergoing dialysis.Future research should explore these dimensions in diverse cultural and geographical contexts to enhance generalizability and support health equity.
基金supported by a grant from the National Natural Science Foundation of China(No.72174130)。
文摘Objectives Nurses’clinical research activities have contributed to optimizing the care process and improving patient outcomes,and generative artificial intelligence(GAI)may help clinical nurses strengthen their research skills.To support research,this study aimed to explore the Chinese nurses’perceptions and experiences of GAI training.Methods This study used a descriptive qualitative design.The China Nurses Network conducted a three-day training session on“GAI for Nursing Research”theme,we selected 23 nurses by a convenience sampling method among participating in the training.The researchers conducted three focus group interviews at the end of each day.All focus groups were interviewed face-to-face to facilitate interaction,data collection,and observation.The data were analyzed using conventional content analysis and coded manually.Results The results showed that nurses’use of GAI to support scientific research was dynamic and characterized by evolving perceptions and practices.Four themes and 11 sub-themes emerged from the analysis:1)utilization efficacy:cope with research ability,affected by many factors;2)booster research:growth and challenges go hand in hand;3)role reversal:from GAI-dominated to nurse-dominated;4)beautiful dream:more features on research,more assistants on clinical care.Conclusions The effectiveness of GAI in supporting clinical nurses in conducting research is mainly limited by differences in personal research literacy,lack of ethical regulation,and information accuracy.In the future,it is necessary to improve nurses’relevant skills through specialized training and promote the standardization of technical regulations to ensure the appropriate application of GAI in nursing research.
文摘BACKGROUND Older patients often experience thirst after general anesthesia for gastrointestinal surgery,particularly during the post-anesthesia care unit(PACU)phase.Thirst not only compromises patient comfort but also induces anxiety,which may hinder recovery.Although previous studies have addressed postoperative thirst,research specifically examining thirst experiences and nursing needs in older patients remains limited.AIM To explore thirst experiences and nursing needs of older PACU patients following gastrointestinal surgery,aiming to inform targeted interventions.METHODS This study employed a phenomenological approach within a qualitative research framework.A purposeful sampling method was used to select 12 older patients who underwent gastrointestinal surgery under general anesthesia at a tertiary hospital in Shanghai between November and December 2024.Data were collected through semi-structured interviews and analyzed using Colaizzi’s seven-step method.Themes were extracted from the interview data.RESULTS Analysis of the interview data identified four main themes and eight subthemes:(1)Intense sensations of thirst post-surgery(subjective experience of thirst and duration of thirst);(2)Emotional experiences of thirst-related discomfort(anxiety,irritability,and helplessness);(3)Practical challenges in relieving thirst(limitations of current interventions and nursing response time);and(4)Patient expectations of nursing care(desire for more timely interventions and expectation for more proactive attention from nursing staff).CONCLUSION Older patients frequently experience substantial thirst discomfort after gastrointestinal surgery under general anesthesia.A multidisciplinary perioperative intervention plan is essential to alleviate these symptoms and enhance postoperative comfort.
文摘Objectives:At the time of the bombing of the federal building in Oklahoma City,Oklahoma(OKC),it was the deadliest terrorist attack in the United States of America.Available research on this incident,and in general,has been quantitative,using deductive methods.The purpose of the current study was to systematically examine professional disaster response workers’emotions elicited spontaneously and in detail as they were experienced over time after a major disaster.This qualitative study will add to existing knowledge of psychopathology and the psychosocial effects of disasters on professional responders,which have not been explored by prior quantitative studies.Methods:A volunteer sample of 181 rescue and recovery workers for the 1995 bombing of the OKC Murrah Federal Building was interviewed approximately 3 years after the bombing.These responders were asked open-ended questions to elicit undirected responses in their own words.In these interviews,they were prompted to describe their feelings at 3 distinct time points:upon their arrival at the bombing scene(“immediately”),in the first 1 week following the bombing,and currently at the time of the interview(“now”).Results:Data items were coded into the 5 themes:Anticipation,Shock and awe,Negative reactions,Positive reactions,and Emotionally unengaged/unaffected.The emotional themes of the responders mapped into 2 conceptual domains.The first domain is related to common human responses to horrible events such as the bombing.The second domain is related to the professional identities of the disaster responders.Conclusions:Specific aspects of the professional responder roles asserted themselves,shaping their emotional responses distinctly from those of the bomb blast survivors.This study identified both negative and positive emotions,which may help identify risk for or resistance to posttraumatic stress disorder(PTSD).
基金This research received grant from 2022 Undergraduate Education and Teaching Research Project of Fujian Medical University(22028).
文摘Objective:This study aimed to investigate the learning expectations of undergraduate nursing students regarding the flipped Health Assessment course.Methods:This descriptive,qualitative study was conducted at a medical university in Fuzhou,Fujian Province,China.An interview outline was designed based on the core dimensions of Expectation Confirmation Theory(expectation sources,expectation content,and expectation importance).Thirty second-year undergraduate nursing students who had completed first-year basic medical courses and were about to take the flipped Health Assessment course were interviewed between June and July 2022.Interview data were analyzed using qualitative content analysis.Results:Five major themes and thirteen subthemes were identified.Theme 1 was expectation sourcesperceived learning difficulties from past experiences,which included four subthemes:insufficient autonomous learning ability,confusion regarding learning methods,insufficient engagement in learning,and low professional identity.Theme 2 included knowledge and information expectations,which comprised three subthemes:knowledge to improve professional competence,knowledge to enhance academic competitiveness,and knowledge to boost self-efficacy.Theme 3 comprised logical expectations and included two subthemes:flexible teaching methods and efficient instructional tools.Theme 4,pleasure expectations,included two subthemes:vivid teaching styles and diversified teaching evaluations.Theme 5 comprised professional value expectations and included two subthemes:teachers'responsible professional attitudes and gentle emotional support.Conclusion:Students'learning expectations originate from perceived learning difficulties,such as insufficient learning autonomy and engagement,confusion about learning methods,and a lack of professional identity.They showed various learning expectations for the flipped Health Assessment course,including knowledge and information,logic,pleasure,and professional value expectations.
基金2024 Annual project of National Social Science Foundation“Research on Problem Identification and Governance Countermeasures of Minor Mental Health Network Support”(Project No.:24BXW044).
文摘Objective:This study aims to explore the experiences of social alienation among adolescents with depression,providing practical This study aims to explore the experiences of social alienation among adolescents with depression,providing practical guidance for improving their interpersonal relationships and facilitating their reintegration into society.Methods:This qualitative research was conducted following the conventional content analysis method.20 adolescents with depression were employed to select from June to August 2024 for face-to-face semi-structured interviews.The collected data were analyzed using Colaizzi's seven-step method.Results:Three themes and eight sub-themes were analyzed and identified:individual level(feelings of helplessness and powerlessness,cognitive distortion,avoidance and withdrawal),family level(lack of family awareness,family conflict),social level(limitations of academic stress and social circle,lack and degradation of skills,generalization of virtual reality,social“stigma”).Conclusion:Adolescents with depression experience complex social alienation.Healthcare providers should enhance their self-awareness and social adaptation skills,improve family dynamics,and provide a comprehensive range of services and services to help them to cope with the challenges of depression.Healthcare providers should enhance their self-awareness and social adaptation skills,improve family dynamics,strengthen communication,bolster family support systems,and collaborate to develop comprehensive social networks and psychological services.This will create a supportive social atmosphere to help adolescents gradually alleviate their feelings of social alienation.
基金This study was supported by Kerman University of medical sciences,with grant number:95000387.
文摘Objective:To explain barriers to childbearing among working women from the perspective of working women and midwives.Methods:In this study,a qualitative study of content analysis method was employed.The study was conducted from December 2021 to July 2022.The population consisted of working women and midwives.Data collection was conducted using semi-structured interviews and continued until data saturation was achieved.Through purposeful sampling,10 women and 11 midwives were selected as the sample.The data were analyzed by Graneheim and Lundman content analysis approach.For more rigorous,Nvivo 14 software was also used for data management,analysis,coding and organization of data.Results:This study included 21 women(10 women employees and 11 midwives)aged 25-43 years(for women employees)and 27-56 years(for midwives)with 1 to 34 years of job experience.In examination of participant’views about the barriers to childbearing,1124 codes were identified,of which we managed to extract six subthemes and two themes.Two themes emerged:“lack of social support and lack of family support”.Subthemes of“lack of social support”were“inadequate leave,long distance from workplace,and insufficient wages”.Subthemes of“lack of family support”were“lack of spouse support,lack of spouse's family support,and lack of their family support”.Conclusions:Different socioeconomic factors influence childbearing tendency in working women.Social and family supports play an important role in this regard.It is important to take these factors into account at the time of policymaking in the field of midwifery.
基金supported by School Research Grant of Tung Wah College(SRG210401).
文摘Objective:To gain insight into the potential factors that may cause perceived stress and anxiety in simulation education.Methods:A secondary qualitative analysis study using qualitative thematic synthesis.A local higher education institution that conducted pre-registration nursing programs.A total of 189 undergraduate nursing students that were never attained any clinical placement prior to the parent study.Focus group interviews were conducted to collect data that were then transcribed and analyzed through the qualitative thematic synthesis approach to develop themes.Results:Three themes were emerged from the participants’simulation experiences in terms of peoples(the observers vs the observed),actions(the prepared vs the unprepared),and settings(the realism vs the simulation).Conclusions:By considering and reviewing the current design and development of the simulation practice,the findings of this study contribute to the body of knowledge with valuable insights on stress and anxiety that may affect students’learning in simulation.
文摘Objective:Patient safety culture is a concern in every healthcare organization,therefore,the healthcare leadership is encountering issues related to patient safety across the globe.In India,there is limited research and information about patient safety culture among healthcare stakeholders and there is relatively little qualitative research available that captures the factors of patient safety culture.Hence,this study aims to explore the perception of healthcare professionals on patient safety culture.Methods:An exploratory qualitative study design was adopted in a tertiary care hospital.Structured focus group discussion(FGD)(n=4)among healthcare professionals and two in-depth interview focus groups were audio-recorded and transcribed.Two coders reviewed transcripts using the editing approach and organized codes into themes.The data were analyzed through MAXQDA 2022(VERBI Software GmbH,Berlin,Germany),qualitative data analysis software,and descriptive analysis technique.The main codes and themes were generated using inductive and deductive method and smart coding was done.Results:Overall,there were 190 unique mentions of codes related to patient safety culture from 4 FGDs.They were categorized into 6 major themes and subcodes were derived via smart coding using the MAXQDA software.“Resources and constraints”was the most prominent code,followed by management support,manpower shortage,burnout,and lack of personnel commitment.Conclusions:The study highlights significant gaps in patient safety culture within the healthcare setting,with resource constraints,management support,and manpower shortages emerging as critical challenges.Burnout and lack of personnel commitment further exacerbate these issues,underscoring the need for targeted interventions.
基金supported by National Natural Science Foundation of China(Grant No.:32301161)the Natural Scientific Foundation of Hunan Province,China(Grant No.:2023JJ60052)+3 种基金the Scientific Research Project of Hunan Provincial Health Commission,China(Grant No.:202112062218,20190161)the Scientific Research Project of Hunan Provincial Department of Education,China(Grant No.:22B0455)the Clinical“4310”Project of the University of South China,China(Grant No.:20224310NHYCG02)the Doctoral Scientific Research Foundation of University of South China,China(Grant No.:200XQD042).
文摘Infrared(IR)spectroscopy,a technique within the realm of molecular vibrational spectroscopy,furnishes distinctive chemical signatures pivotal for both structural analysis and compound identification.A notable challenge emerges from the misalignment between the mid-IR light wavelength range and molecular dimensions,culminating in a constrained absorption cross-section and diminished vibrational absorption coefficients(Supplementary data).
文摘Objective:To explore the perspectives of high-risk pregnant women regarding the home care program for high-risk pregnancies.Methods:This qualitative study was conducted in a university hospital and 5 comprehensive health centers in Ahvaz,Iran,from February 2023 to July 2023.The sampling method used was purposive sampling considering the maximum possible diversity,which continued until data saturation.11 in-depth and semistructured interviews were conducted with high-risk pregnant women.Data were simultaneously analysed using Graneheim and Lundman content analysis by MAXQDA version 20 software.Results:Data analysis extracted 3 themes,12 categories,and 26 subcategories.The themes and categories were“high-risk pregnancy and related issues(pursuing treatment and paying attention to the high-risk situation by pregnant women,psychological aspect of high-risk pregnancy on pregnant women and caregivers,emotional and physical aspects of high-risk pregnancy,the consequences of high-risk pregnancy on the people around them and empowerment of high-risk pregnant women)”,“support dimension(family and community support for high-risk pregnant women)”,and“framework of program(acculturalization,means and equipment,security,manpower and the necessary arrangements for the home care)”.Conclusions:High-risk pregnancy affects various aspects of the lives of high-risk pregnant women and their families.Creating the necessary conditions for providing care at home plays a vital role in supporting high-risk pregnant women and removing related obstacles to receiving prenatal care in person.It will be especially beneficial for high-risk pregnant women in low-income areas.
文摘Background and Objectives:Non-Communicable Diseases(NCDs)represent a significant public health burden in China,particularly in cold regions where environmental factors further limit access to healthcare.To address this challenge,China has implemented the National Essential Public Health Service Package(NEPHSP)to improve NCD management through primary healthcare services.However,research on the effectiveness of NEPHSP in cold regions is limited.This study aims to identify the key factors influencing the implementation of NCD management services in cold regions,focusing on both barriers and facilitators.Methods:This qualitative study was conducted in three purposively selected cold regions in Northeast China(Harbin,Qiqihar,and Jiamusi).Data were collected through semi-structured interviews and focus group discussions,guided by the RE-AIM framework.Participants included local health administrators,primary healthcare workers,and community-dwelling individuals with NCDs.Thematic analysis was employed to code and extract key themes from the data.Results:A total of 72 participants(59.7%female,mean age 49.1±6.24 years)took part in the study.Major barriers to NCD management included low health literacy,limited awareness of NCDs,insufficient medical resources,poor public awareness,inadequate multisectoral collaboration,and the absence of effective audit and feedback systems.Key facilitators included the affordability and accessibility of primary healthcare services,strong patient-physician relationships,and the high priority given to NCD management by local governments.Furthermore,harsh environmental conditions and limited healthcare accessibility in cold regions complicated the management of NCDs,highlighting the need for tailored interventions that address the specific challenges of these regions.Conclusion:This study identifies significant facilitators and barriers to the implementation of NCD management services under the NEPHSP in cold regions of China.The unique challenges posed by cold climates necessitate the adaptation of healthcare services to local needs,with a focus on improving health literacy,enhancing healthcare infrastructure,and fostering multisectoral collaboration.These findings provide valuable insights for optimizing NCD prevention and management strategies within China's primary healthcare system.
文摘Objective:Through interviews with oral specialist nurses,to explore the influencing factors of training transfer for oral specialist nurses,and provide references for scientifically improving the effect of specialist nurse training transfer.Methods:Using purposive sampling and descriptive qualitative research,15 oral specialist nurses from two tertiary hospitals in Guangdong Province were selected for semi-structured interviews,and data analysis was performed.Results:After collating and analyzing the interview data,three types of factors affecting the training transfer of oral specialist nurses were proposed,including personal characteristics,training management,and training transfer atmosphere.Conclusion:Managers should formulate a comprehensive and systematic plan based on the influencing factors of oral specialist nurse training transfer to effectively promote training transfer behavior and enhance training transfer effects.
基金Key Projects in the National Science & Technology Pillar Program during the 11thFive-Year Plan Period (Grant No.2008BAI51B02)National Natural Science Fund Project of China (Grant No. 81202904)
文摘Huang-Lian-Jie-Du-Decoction （HLJDD） has been widely used for the treatment of Alzheimer＇s disease （AD） in clinic. However, the relationship between its chemical profile and neuroprotective bioactivity was not clearly clarified yet. In present study, the water extract of HLJDD and subsequent three polarity fractions divided by different reagents were investigated. A total of 17 chromatographic peaks were confirmed by comparison with standards and their UV, MS spectra. Among them, 11 major compounds were determined by HPLC-DAD method with good linear regression relationship （r2, 0.9994-0.9999）, precisions （inter-day precision RSD, 0.79%-1.07%; intra-day precision RSD, 1.59%-2.10%）, repeatability （RSD, 1.66%-3.67%）, stability （RSD, 1.26%-4.77%） and recovery （95.24%-105.41%, RSD, 0.29%-2.69%）. Furthermore, PC12 cells and primary neurons cells were used for the neuroprotective effective assessment of aforementioned four samples from HLJDD. 3＂he total aqueous extract and n-butanol extract of HLJDD presented more significant effects than the other two parts. According to their quality and quantity determination results, iridoids and alkaloids have a positive correlation with the neuroprotective effectiveness of HLJDD.
基金Supported by Important National Science & Technology Specific Pro-jects(2008ZX08012-001)~~
文摘[Objective] The aim was to establish an event-specific qualitative PCR method for transgenic soybean MON89788.[Method] Firstly,the 3′-junction sequence between host plant DNA and integrated DNA of transgenic MON89788 soybean was isolated using thermal asymmetric interlaced-PCR （TAIL-PCR）,and the specific PCR primers were designed based on the 3′-junction sequence.Secondly,the specificity and sensitivity of the qualitative PCR detection methods employing these primers were tested.[Result] 1 142-bp 3′-junction sequence was obtained.According to the sequence,event-specific qualitative PCR method was established,amplifying a 170-bp product specifically from MON89788 event,and the limit of detection was 0.05%,approximately 40 initial template copies.[Conclusion] The method was highly specific,sensitive,and suitable for detection of MON89788 event.
文摘Given the great strides that China＇s education sector has made in recent decades, it can be expected that the overall workforce quality of exiting China＇s labor market in coming one or two decades will be relatively low, while the overall quality of workforce newly entering into China＇s labor market will be high. As the new, bettereducated generation takes over, China＇s workforce quality will be vastly improved. This in turn will promote economic growth. We refer to economic growth arising from improved workforce quality as qualitative demographic dividend. Using the computable general equilibrium （CGE） model, this paper investigates the relationship between workforce quality improvements and economic growth. According to the model＇s results, an improvement in workforce quality will raise the economic growth rate by about two percentage points per annum between 2016 and 2020 and by 10 percentage points cumulatively by 2020. In other words, GDP will be 1.1 times the level of baseline GDP by 2020 due to the improved education levels. Given different production functions across sectors, the improvement of workforce quality will affect different sectors in different ways. On the whole, the improvement of workforce quality is more favorable to the development of capital-intensive sectors and sectors with rapid technology progress. According to this paper, considering the improvement of workforce quality, we cannot conclude that China＇s potential economic growth rate has already begun to decline. Despite diminishing conventional quantitative demographic dividends, China ＂s qualitative demographic dividends will keep rising. Qualitative demographic dividends will further push forward China＇s industrial restructuring and the strategic transition of industrial competitiveness from quantitative to qualitative and from an extensive to an intensive pattern of development.
基金supported by the National Natural Science Foundation of China (Grant No. 30700494)the Principal Fund of South China Agricultural University, China (Grant No. 2003K053)
文摘The development of a core collection could enhance the utilization of germplasm collections in crop improvement programs and simplify their management. Selection of an appropriate sampling strategy is an important prerequisite to construct a core collection with appropriate size in order to adequately represent the genetic spectrum and maximally capture the genetic diversity in available crop collections. The present study was initiated to construct nested core collections to determine the appropriate sample size to represent the genetic diversity of rice landrace collection based on 15 quantitative traits and 34 qualitative traits of 2 262 rice accessions. The results showed that 50-225 nested core collections, whose sampling rate was 2.2%-9.9%, were sufficient to maintain the maximum genetic diversity of the initial collections. Of these, 150 accessions （6.6%） could capture the maximal genetic diversity of the initial collection. Three data types, i.e. qualitative traits （QT1）, quantitative traits （QT2） and integrated qualitative and quantitative traits （QTT）, were compared for their efficiency in constructing core collections based on the weighted pair-group average method combined with stepwise clustering and preferred sampling on adjusted Euclidean distances. Every combining scheme constructed eight rice core collections （225, 200, 175, 150, 125, 100, 75 and 50）. The results showed that the QTT data was the best in constructing a core collection as indicated by the genetic diversity of core collections. A core collection constructed only on the information of QT1 could not represent the initial collection effectively. QTT should be used together to construct a productive core collection.
基金supported by National Natural Science Foundation of China(No.61075073 and 61375079)
文摘A new approach to model and control an unknown system using subjective uncertain rules is proposed. This method is established by combining the grey system theory and the qualitative simulation method. The proposed approach mainly contains three steps. In the first step, subjective uncertain rules are accumulated gradually during cognizing the system; the mapping relations between the system inputs and outputs are built and represented using the grey qualitative matrix in the second step; in the third step,the generalized whitening function is defined to realize the transformation between qualitative and quantitative information. Besides the theoretical results, two sets of simulations based on a water level control system are conducted comparatively to demonstrate the effectiveness of the proposed method. The water level expectation is set to be constant in the first set, while it changes in the second set. The simulation results show that the proposed method tracks the water level expectation well. By combining the proposed method with proportional-integral-derivative(PID) or fuzzy logic controller(FLC), it can be concluded that the system can reach the stable state more quickly and the overshoot can also be reduced compared to using PID or FLC alone.
