Objective:Previous studies have found that Qidi Tangshen granules(QDTS),a combination therapy of supplementing essence(Tianjing,TJ)and unblocking the collaterals(Tongluo,TL),can reduce kidney damage in db/db mice.This...Objective:Previous studies have found that Qidi Tangshen granules(QDTS),a combination therapy of supplementing essence(Tianjing,TJ)and unblocking the collaterals(Tongluo,TL),can reduce kidney damage in db/db mice.This study aimed to explore the effect of QDTS and their separate prescriptions on podocytes in mice with diabetic nephropathy.Methods:The db/db mice were used in this experiment as an animal model,while wild-type C57BL/6J mice were used as normal controls.At the age of 12 weeks,the db/db mice were randomly divided into 5 groups(db/db,db/dbþvalsartan,db/dbþQDTS,db/dbþTJ and db/dbþTL).The urine albumin excretion ratio(UAE)was measured by enzyme-linked immunosorbent assay before and after the intervention.The ultrastructure of the kidney podocytes was observed by transmission electron microscopy.The protein expression levels of nephrin and desmin were detected by immunohistochemistry.Results:QDTS and their separate prescriptions significantly decreased the UAE and attenuated the renal pathological injury.QDTS and their separate prescriptions also reduced the fusion rate of the foot processes and increased the expression of nephrin protein.In contrast,QDTS and their separate prescriptions(TJ and TL)reduced the expression level of desmin protein.Conclusion:QDTS and their separate prescriptions might reduce diabetes-induced renal injury by reducing podocyte damage.The therapeutic effect of QDTS was more pronounced than TJ and TL.展开更多
目的探讨芪地消渴胶囊联合达格列净对糖尿病肾病(diabetic nephropathy,DN)患者肾功能及焦亡相关因子的影响。方法选取121例DN患者分为研究组(n=60)和对照组(n=61)。对照组予以达格列净治疗,研究组给予芪地消渴胶囊联合达格列净治疗。...目的探讨芪地消渴胶囊联合达格列净对糖尿病肾病(diabetic nephropathy,DN)患者肾功能及焦亡相关因子的影响。方法选取121例DN患者分为研究组(n=60)和对照组(n=61)。对照组予以达格列净治疗,研究组给予芪地消渴胶囊联合达格列净治疗。比较两组中医证候积分、空腹血糖(FPG)、餐后2 h血糖(2hPG)、糖化血红蛋白(HbA1c)、尿素氮(BUN)、血肌酐(Scr)、24 h尿蛋白定量(24 h UP)、Nod样受体热蛋白结构域相关蛋白3(NLRP3)、胱天蛋白酶-1(Caspase-1)、白介素-18(IL-18)、白介素-1β(IL-1β)、临床疗效及不良反应。结果治疗后,研究组中医证候积分低于对照组(P<0.05)。研究组HbA1c、2hPG及FBG较对照组降低(P<0.05)。研究组eGFR较对照组增高,BUN、Scr及24 h UP较对照组降低(P<0.05)。研究组NLRP3、Caspase-1、IL-18及IL-1β低于对照组(P<0.05)。研究组临床疗效优于对照组(P<0.05)。两组不良反应发生率比较无统计学差异(P>0.05)。结论芪地消渴胶囊联合达格列净治疗DN安全有效,可改善临床症状、血糖水平及肾功能,其机制可能与调控焦亡相关因子有关。展开更多
目的:观察益肾敛精泄浊法对肝肾阴虚、瘀血湿浊型糖尿病肾病患者的临床疗效。方法:选取门诊及病房肝肾阴虚、瘀血湿浊型糖尿病肾病患者96例,随机分为治疗组和对照组,每组各48例,两组患者均采用常规糖尿病基础治疗,对照组在常规治疗的基...目的:观察益肾敛精泄浊法对肝肾阴虚、瘀血湿浊型糖尿病肾病患者的临床疗效。方法:选取门诊及病房肝肾阴虚、瘀血湿浊型糖尿病肾病患者96例,随机分为治疗组和对照组,每组各48例,两组患者均采用常规糖尿病基础治疗,对照组在常规治疗的基础上加用盐酸贝那普利片(洛汀新)10 mg,每日1次口服;治疗组在对照组治疗的基础上加用芪地降白汤,每日1剂,水煎分2次早晚温服,疗程3个月。分别于治疗前、后检测空腹血糖(FBG)、餐后2 h血糖(2 h PG)、肿瘤坏死因子-α(TNF-α)、白细胞介素-18(IL-18)、白细胞介素-6(IL-6)、超敏C-反应蛋白(Hs-CRP)、24 h尿蛋白定量(24 h Upro)、尿白蛋白排泄率(UAER)。结果:治疗组与对照组治疗后综合疗效比较,治疗组总有效率为89.58%,对照组总有效率为66.67%,治疗组优于对照组(P<0.05);芪地降白汤治疗组患者治疗后24 h Upro、UAER、TNF-α、IL-18、IL-6、Hs-CRP表达水平均降低,优于对照组(P<0.05)。结论:芪地降白汤可以减少尿蛋白排泄,对糖尿病肾病具有显著保护作用,其机制可能与减轻肾脏的炎症损伤有关。展开更多
基金This study was supported by the National Natural Science Foundation of China program(81774273 and 82004275)Beijing Municipal Science and Technology Commission(Z161100001816003).
文摘Objective:Previous studies have found that Qidi Tangshen granules(QDTS),a combination therapy of supplementing essence(Tianjing,TJ)and unblocking the collaterals(Tongluo,TL),can reduce kidney damage in db/db mice.This study aimed to explore the effect of QDTS and their separate prescriptions on podocytes in mice with diabetic nephropathy.Methods:The db/db mice were used in this experiment as an animal model,while wild-type C57BL/6J mice were used as normal controls.At the age of 12 weeks,the db/db mice were randomly divided into 5 groups(db/db,db/dbþvalsartan,db/dbþQDTS,db/dbþTJ and db/dbþTL).The urine albumin excretion ratio(UAE)was measured by enzyme-linked immunosorbent assay before and after the intervention.The ultrastructure of the kidney podocytes was observed by transmission electron microscopy.The protein expression levels of nephrin and desmin were detected by immunohistochemistry.Results:QDTS and their separate prescriptions significantly decreased the UAE and attenuated the renal pathological injury.QDTS and their separate prescriptions also reduced the fusion rate of the foot processes and increased the expression of nephrin protein.In contrast,QDTS and their separate prescriptions(TJ and TL)reduced the expression level of desmin protein.Conclusion:QDTS and their separate prescriptions might reduce diabetes-induced renal injury by reducing podocyte damage.The therapeutic effect of QDTS was more pronounced than TJ and TL.
文摘目的探讨芪地消渴胶囊联合达格列净对糖尿病肾病(diabetic nephropathy,DN)患者肾功能及焦亡相关因子的影响。方法选取121例DN患者分为研究组(n=60)和对照组(n=61)。对照组予以达格列净治疗,研究组给予芪地消渴胶囊联合达格列净治疗。比较两组中医证候积分、空腹血糖(FPG)、餐后2 h血糖(2hPG)、糖化血红蛋白(HbA1c)、尿素氮(BUN)、血肌酐(Scr)、24 h尿蛋白定量(24 h UP)、Nod样受体热蛋白结构域相关蛋白3(NLRP3)、胱天蛋白酶-1(Caspase-1)、白介素-18(IL-18)、白介素-1β(IL-1β)、临床疗效及不良反应。结果治疗后,研究组中医证候积分低于对照组(P<0.05)。研究组HbA1c、2hPG及FBG较对照组降低(P<0.05)。研究组eGFR较对照组增高,BUN、Scr及24 h UP较对照组降低(P<0.05)。研究组NLRP3、Caspase-1、IL-18及IL-1β低于对照组(P<0.05)。研究组临床疗效优于对照组(P<0.05)。两组不良反应发生率比较无统计学差异(P>0.05)。结论芪地消渴胶囊联合达格列净治疗DN安全有效,可改善临床症状、血糖水平及肾功能,其机制可能与调控焦亡相关因子有关。
文摘目的:观察益肾敛精泄浊法对肝肾阴虚、瘀血湿浊型糖尿病肾病患者的临床疗效。方法:选取门诊及病房肝肾阴虚、瘀血湿浊型糖尿病肾病患者96例,随机分为治疗组和对照组,每组各48例,两组患者均采用常规糖尿病基础治疗,对照组在常规治疗的基础上加用盐酸贝那普利片(洛汀新)10 mg,每日1次口服;治疗组在对照组治疗的基础上加用芪地降白汤,每日1剂,水煎分2次早晚温服,疗程3个月。分别于治疗前、后检测空腹血糖(FBG)、餐后2 h血糖(2 h PG)、肿瘤坏死因子-α(TNF-α)、白细胞介素-18(IL-18)、白细胞介素-6(IL-6)、超敏C-反应蛋白(Hs-CRP)、24 h尿蛋白定量(24 h Upro)、尿白蛋白排泄率(UAER)。结果:治疗组与对照组治疗后综合疗效比较,治疗组总有效率为89.58%,对照组总有效率为66.67%,治疗组优于对照组(P<0.05);芪地降白汤治疗组患者治疗后24 h Upro、UAER、TNF-α、IL-18、IL-6、Hs-CRP表达水平均降低,优于对照组(P<0.05)。结论:芪地降白汤可以减少尿蛋白排泄,对糖尿病肾病具有显著保护作用,其机制可能与减轻肾脏的炎症损伤有关。
