As an anticonvulsant,the influences of Qingyangshen(QYS)on the development of brainand memory behavior were observed in four-week-old male rats.The dosage of QYS was100 mg/kg/QOD and the period of administration was o...As an anticonvulsant,the influences of Qingyangshen(QYS)on the development of brainand memory behavior were observed in four-week-old male rats.The dosage of QYS was100 mg/kg/QOD and the period of administration was one month.The results showedthat QYS had less influence on the development of body weight and brain wet weight inyoung-aged rats than diphenylhydantoin(DPH).However,QYS inhibited the establish-ment of passive avoidance conditional reflex and had influence on memory behavior inyoung-aged rats.展开更多
The concentrations of central neuropeptides,somatostatin(SS) and substance-P (SP),were determined in the different brain regions of young-aged male rats after a long-term ad-m inistration of anticonvulsants Qingyangsh...The concentrations of central neuropeptides,somatostatin(SS) and substance-P (SP),were determined in the different brain regions of young-aged male rats after a long-term ad-m inistration of anticonvulsants Qingyangshen(QYS),Diphenylhydantoin(DPH),andCarbamazepine(CBZ).The results were compared with Pentylenetetrazol(PTZ)-inducedseizure model and normal saline-treated controls.No effects of QYS on the concentrationsof SS and SP were found in the rats of four-week or eight week groups.Both of DPH andPTZ increased the SS levels in the midbrain of rats in four-week gronp.DPH,CBZ,andPTZ also increased the SP levels in the cerebral cortex,striatum,and brain stem of rats ineight-week group.Our present data indicated that the central neuropeptides SS and SPwere involved in the processes of epilepsy and antiepilepsy.Since QYS did not influence thecontents of SS and SP after a long-term administration,it suggested that theanticonvulsant mechanism of QYS may be different from those of DPH and CBZ,i.e.itmay be not due to its effect on the central neuropeptide pathway.展开更多
The effects of QYS on the functions of central GABAergic system in mice brain were investi-gated by determining the release and uptake of label GABA and Glu in the brain slice andextractive synaptic membrane.The drama...The effects of QYS on the functions of central GABAergic system in mice brain were investi-gated by determining the release and uptake of label GABA and Glu in the brain slice andextractive synaptic membrane.The dramatic actions of QYS in the oral group were showed bythe increasing releases of ~3H-GABA and ~3H-Glu after high K^+ stimulation in the anterior partof hemispheres slices.However,the GAD activity in the hippocampus slice of QYS-treatedgroup was not influenced as the PTZ-treated group.No effect of QYS on the uptakes of^3H-GABA and ~3H-Glu in the extractive synaptic membrane was found in the intraperitonealgroup.These results suggested that QYS does influence the functions of central GABAergic sys-tern due to the increasing releases of GABA and Glu in the anterior part of hemispheres,whichmay play an important role in the anticonvulsant action of QYS.展开更多
Background:Qingyangshen(Cynanchum otophyllum C.K.Schneid)is a folk drug for treating depression and other mental disorders induced by social defeat stress.Neuroplasticity in the hippocampus is essential for the modula...Background:Qingyangshen(Cynanchum otophyllum C.K.Schneid)is a folk drug for treating depression and other mental disorders induced by social defeat stress.Neuroplasticity in the hippocampus is essential for the modulation of cognition and emotion,and its impairment may contribute to the development and progression of depression.Our previous studies have found that Qingyangshen glycosides(QYS)can improve depression-like behavior in social failure mouse models,mainly through PGC-1α/FNDC5/BDNF signaling pathways activation,but its effects and mechanisms on hippocampal neuroplasticity remain unknown.Methods:Chronic social defeat stress(CSDS)was used to induce social defeat in mice.Morphological changes in the hippocampus were observed by H&E staining and Golgi staining.Immunofluorescence double staining was used to detect the expression of synaptophysin(SYN)and postsynaptic density protein-95(PSD-95),while western blot was employed to evaluate PSD-95,SYN,and doublecortin(DCX)proteins.The pathological processing of social defeat and the therapeutic effects of QYS on it was confirmed through behavioral assessment associated with morpho-logic observation.Results:During the whole study,the sucrose preference indices and OFT activity time of CSDS mice were significantly decreased(p≤0.05),and the tail suspension immobil-ity time was significantly increased(p≤0.05),suggesting that the mice had significant depressive symptoms.Treatment with QYS(25,50,and 100 mg/kg)significantly al-leviated depressive symptoms in CSDS mice,which was demonstrated by significantly(p≤0.05 or p≤0.01)reducing the duration of tail-hanging immobility and increasing the tendency of sucrose preference indices and OFT activity time.QYS treatment also significantly increased the expression of DCX,PSD-95,and SYN proteins,which play a crucial role in depression.Conclusions:QYS alleviated these symptoms by enhancing hippocampal neuroplasti-city through upregulating the expression of synapse-associated proteins(SAPs).The therapeutic mechanism of QYS may involve modulating the neuroplasticity of hip-pocampus neurons by altering the expression of SAPs.展开更多
文摘As an anticonvulsant,the influences of Qingyangshen(QYS)on the development of brainand memory behavior were observed in four-week-old male rats.The dosage of QYS was100 mg/kg/QOD and the period of administration was one month.The results showedthat QYS had less influence on the development of body weight and brain wet weight inyoung-aged rats than diphenylhydantoin(DPH).However,QYS inhibited the establish-ment of passive avoidance conditional reflex and had influence on memory behavior inyoung-aged rats.
文摘The concentrations of central neuropeptides,somatostatin(SS) and substance-P (SP),were determined in the different brain regions of young-aged male rats after a long-term ad-m inistration of anticonvulsants Qingyangshen(QYS),Diphenylhydantoin(DPH),andCarbamazepine(CBZ).The results were compared with Pentylenetetrazol(PTZ)-inducedseizure model and normal saline-treated controls.No effects of QYS on the concentrationsof SS and SP were found in the rats of four-week or eight week groups.Both of DPH andPTZ increased the SS levels in the midbrain of rats in four-week gronp.DPH,CBZ,andPTZ also increased the SP levels in the cerebral cortex,striatum,and brain stem of rats ineight-week group.Our present data indicated that the central neuropeptides SS and SPwere involved in the processes of epilepsy and antiepilepsy.Since QYS did not influence thecontents of SS and SP after a long-term administration,it suggested that theanticonvulsant mechanism of QYS may be different from those of DPH and CBZ,i.e.itmay be not due to its effect on the central neuropeptide pathway.
文摘The effects of QYS on the functions of central GABAergic system in mice brain were investi-gated by determining the release and uptake of label GABA and Glu in the brain slice andextractive synaptic membrane.The dramatic actions of QYS in the oral group were showed bythe increasing releases of ~3H-GABA and ~3H-Glu after high K^+ stimulation in the anterior partof hemispheres slices.However,the GAD activity in the hippocampus slice of QYS-treatedgroup was not influenced as the PTZ-treated group.No effect of QYS on the uptakes of^3H-GABA and ~3H-Glu in the extractive synaptic membrane was found in the intraperitonealgroup.These results suggested that QYS does influence the functions of central GABAergic sys-tern due to the increasing releases of GABA and Glu in the anterior part of hemispheres,whichmay play an important role in the anticonvulsant action of QYS.
基金Guizhou Administration of TCM,Traditional Chinese medicine,Ethnic Medicine Science and Technology Research Special Project,Grant/Award Number:QZYY-2022-008Subsidy Fund for Scientific Research and Innovation Exploration Project,National Natural Science Foundation of China of Guizhou University of Traditional Chinese Medicine,Grant/Award Number:2018YFC170810502+1 种基金Science and Technology Fund Project of Guizhou Provincial Health Commission,Grant/Award Number:gzwkj2023-147Development of animal models for drug discovery-brain diseases.National Research and Development Project of China,MOST,Grant/Award Number:2023YFF0724802.
文摘Background:Qingyangshen(Cynanchum otophyllum C.K.Schneid)is a folk drug for treating depression and other mental disorders induced by social defeat stress.Neuroplasticity in the hippocampus is essential for the modulation of cognition and emotion,and its impairment may contribute to the development and progression of depression.Our previous studies have found that Qingyangshen glycosides(QYS)can improve depression-like behavior in social failure mouse models,mainly through PGC-1α/FNDC5/BDNF signaling pathways activation,but its effects and mechanisms on hippocampal neuroplasticity remain unknown.Methods:Chronic social defeat stress(CSDS)was used to induce social defeat in mice.Morphological changes in the hippocampus were observed by H&E staining and Golgi staining.Immunofluorescence double staining was used to detect the expression of synaptophysin(SYN)and postsynaptic density protein-95(PSD-95),while western blot was employed to evaluate PSD-95,SYN,and doublecortin(DCX)proteins.The pathological processing of social defeat and the therapeutic effects of QYS on it was confirmed through behavioral assessment associated with morpho-logic observation.Results:During the whole study,the sucrose preference indices and OFT activity time of CSDS mice were significantly decreased(p≤0.05),and the tail suspension immobil-ity time was significantly increased(p≤0.05),suggesting that the mice had significant depressive symptoms.Treatment with QYS(25,50,and 100 mg/kg)significantly al-leviated depressive symptoms in CSDS mice,which was demonstrated by significantly(p≤0.05 or p≤0.01)reducing the duration of tail-hanging immobility and increasing the tendency of sucrose preference indices and OFT activity time.QYS treatment also significantly increased the expression of DCX,PSD-95,and SYN proteins,which play a crucial role in depression.Conclusions:QYS alleviated these symptoms by enhancing hippocampal neuroplasti-city through upregulating the expression of synapse-associated proteins(SAPs).The therapeutic mechanism of QYS may involve modulating the neuroplasticity of hip-pocampus neurons by altering the expression of SAPs.
