The rapid prediction of seepage mass flow in soil is essential for understanding fluid transport in porous media.This study proposes a new method for fast prediction of soil seepage mass flow by combining mesoscopic m...The rapid prediction of seepage mass flow in soil is essential for understanding fluid transport in porous media.This study proposes a new method for fast prediction of soil seepage mass flow by combining mesoscopic modeling with deep learning.Porous media structures were generated using the Quartet Structure Generation Set(QSGS)method,and a mesoscopic-scale seepage calculation model was applied to compute flow rates.These results were then used to train a deep learning model for rapid prediction.The analysis shows that larger average pore diameters lead to higher internal flow velocities and mass flow rates,while pressure drops significantly at the throats of fine pores.The trained model predicts seepage mass flow rates with deviations within±20%,achieving a root mean square error of 0.24261 and an average deviation of-0.02197.Importantly,the method performs well even with limited training data,though image-based deep learning approaches may yield better accuracy when larger datasets are available.展开更多
The Qushi’ang granodiorite(QSG)is located at the central east of the ophiolitic melange belt in the East Kunlun Orogenic Belt(EKOB)in the northern margin of the Qinghai-Tibetan Plateau.LA-MC-ICP-MS zircon U–Pb datin...The Qushi’ang granodiorite(QSG)is located at the central east of the ophiolitic melange belt in the East Kunlun Orogenic Belt(EKOB)in the northern margin of the Qinghai-Tibetan Plateau.LA-MC-ICP-MS zircon U–Pb dating suggests that the granodiorite and mafic microgranular enclaves(MMEs)crystallized 246.61±0.62 and 245.45±0.9 Ma ago,respectively.Granodiorite,porphyritic diorite,and MMEs are metaluminous and medium-K calk-alkaline series,with island-arc magma features,such as LILE enrichment and HFSE depletion.The porphyritic diorite has high Cr(13.50 ppm to 59.01 ppm),Ni(228.53 ppm to 261.29 ppm),and Mg~#(46–54).Granodiorite and porphyritic diorite have similar mineral compositions and evolved major and trace elements contents,particularly Cr and Ni,both of which are significantly higher than that in granites of the same period.The crystallization age of MMEs is close to that of granodiorite,and their major and trace elements contents are in-between porphyritic diorite and granodiorite.The results suggest that the original mafic magma,which was the product of mantle melting by subduction process,intruded into the lower crust(Kuhai Rock Group),resulting in the formation of granodiorite.Countinous intrusion of mafic magma into the unconsolidated granodiorite formed MMEs and porphyritic diorite.The granodiorite reformed by late-stage strike-slip faulting tectonic event indicates that the strike-slip fault of Middle Kunlun and the collision of the Bayanhar block with East Kunlun were later than 246 Ma.Therefore,the formation of the QSG not only indicates the critical period of evolution of East Kunlun but also represents the tectonic transition from oceanic crust subduction to slab breaking.展开更多
AIM:To study the effect of hepatitis C virus nonstructural region 3 (HCV NS3) protein on proliferation and transformation of normal human liver cell line. METHODS: QSG7701 cells were transfected with pRcHCNS3-5', ...AIM:To study the effect of hepatitis C virus nonstructural region 3 (HCV NS3) protein on proliferation and transformation of normal human liver cell line. METHODS: QSG7701 cells were transfected with pRcHCNS3-5', pRcHCNS3-3'and pRcCMV using lipofectamine transfecting technique and selected with G418 method. Expression of HCV NS3 protein was determined by immunohistochemistry. Biologic characteristics of transfected cells were evaluated by population doubling time and soft agar assays. Activation of MAPK was analyzed using Western blot with phosphospecific monoclonal antibody against dually phosphorylated MAPK. RESULTS: QSG7701 cells transfected with pRcHCNS3-5' showed strong intracellular expression of HCVNS3 protein, and the positive signal was localized in cytoplasm.The expressing strength of HCVNS3 protein in pRcHCNS3-3'-transfected cells was weaker than that in pRcHCNS3-5'-transfected cells. The population doubling time in the transfected cells with pRcHCNS3-5' (12 h) was much shorter than those with pRcHCNS3-3', pRcCMV and normal cells (24, 26, 28 h, respectively) (P<0.01). The transfected cells with pRcHCNS3-5' showed much more anchorage independent colonies than that in those with pRcHCNS3-3' and pRcCMV (P<0.01). The cloning efficiencies of transfected cells with pRcHCNS3-5', pRcHCNS3-3', pRcCMV and controls were 33%, 1.33%, 1.46%, 1.11% respectively. The level of phosphorylated MAPK in the cells with pRcHCNS3-5' was much higher than that in those with pRcHCNS3-3'and pRcCMV and normal cells (P<0.01). CONCLUSION: The results suggest that (1) QSG7701 cells are a better human liver cell line for investigating' the pathogenesis of HCV NS3 protein. (2) 5' region of the HCV genome segment encoding HCV NS3 is involved in cell growth and cell phenotype. (3) HCV NS3 N-terminal peptide may up-regulate the activation of MAPK, but not affect the expression of MAPK.展开更多
基金Dynamics of CO_(2) Leakage and Seepage in Wellbores Under Reservoir Stimulation,grant number YJCCUS25SFW0004.
文摘The rapid prediction of seepage mass flow in soil is essential for understanding fluid transport in porous media.This study proposes a new method for fast prediction of soil seepage mass flow by combining mesoscopic modeling with deep learning.Porous media structures were generated using the Quartet Structure Generation Set(QSGS)method,and a mesoscopic-scale seepage calculation model was applied to compute flow rates.These results were then used to train a deep learning model for rapid prediction.The analysis shows that larger average pore diameters lead to higher internal flow velocities and mass flow rates,while pressure drops significantly at the throats of fine pores.The trained model predicts seepage mass flow rates with deviations within±20%,achieving a root mean square error of 0.24261 and an average deviation of-0.02197.Importantly,the method performs well even with limited training data,though image-based deep learning approaches may yield better accuracy when larger datasets are available.
基金jointly supported by the National Science Foundation of China(Grant No.,41472191,41502191,41172186,40972136)the Special Fund for Basic Scientific Research of Central Colleages,Chang'an University(Grant Nos.310827161002,310827161006)the Commonweal Geological Survey,the Aluminum Corporation of China and the Land-Resources Department of Qinghai Province(Grant No.,200801)
文摘The Qushi’ang granodiorite(QSG)is located at the central east of the ophiolitic melange belt in the East Kunlun Orogenic Belt(EKOB)in the northern margin of the Qinghai-Tibetan Plateau.LA-MC-ICP-MS zircon U–Pb dating suggests that the granodiorite and mafic microgranular enclaves(MMEs)crystallized 246.61±0.62 and 245.45±0.9 Ma ago,respectively.Granodiorite,porphyritic diorite,and MMEs are metaluminous and medium-K calk-alkaline series,with island-arc magma features,such as LILE enrichment and HFSE depletion.The porphyritic diorite has high Cr(13.50 ppm to 59.01 ppm),Ni(228.53 ppm to 261.29 ppm),and Mg~#(46–54).Granodiorite and porphyritic diorite have similar mineral compositions and evolved major and trace elements contents,particularly Cr and Ni,both of which are significantly higher than that in granites of the same period.The crystallization age of MMEs is close to that of granodiorite,and their major and trace elements contents are in-between porphyritic diorite and granodiorite.The results suggest that the original mafic magma,which was the product of mantle melting by subduction process,intruded into the lower crust(Kuhai Rock Group),resulting in the formation of granodiorite.Countinous intrusion of mafic magma into the unconsolidated granodiorite formed MMEs and porphyritic diorite.The granodiorite reformed by late-stage strike-slip faulting tectonic event indicates that the strike-slip fault of Middle Kunlun and the collision of the Bayanhar block with East Kunlun were later than 246 Ma.Therefore,the formation of the QSG not only indicates the critical period of evolution of East Kunlun but also represents the tectonic transition from oceanic crust subduction to slab breaking.
基金Supported by the National Natural Science Foundation of China,No. 30270601
文摘AIM:To study the effect of hepatitis C virus nonstructural region 3 (HCV NS3) protein on proliferation and transformation of normal human liver cell line. METHODS: QSG7701 cells were transfected with pRcHCNS3-5', pRcHCNS3-3'and pRcCMV using lipofectamine transfecting technique and selected with G418 method. Expression of HCV NS3 protein was determined by immunohistochemistry. Biologic characteristics of transfected cells were evaluated by population doubling time and soft agar assays. Activation of MAPK was analyzed using Western blot with phosphospecific monoclonal antibody against dually phosphorylated MAPK. RESULTS: QSG7701 cells transfected with pRcHCNS3-5' showed strong intracellular expression of HCVNS3 protein, and the positive signal was localized in cytoplasm.The expressing strength of HCVNS3 protein in pRcHCNS3-3'-transfected cells was weaker than that in pRcHCNS3-5'-transfected cells. The population doubling time in the transfected cells with pRcHCNS3-5' (12 h) was much shorter than those with pRcHCNS3-3', pRcCMV and normal cells (24, 26, 28 h, respectively) (P<0.01). The transfected cells with pRcHCNS3-5' showed much more anchorage independent colonies than that in those with pRcHCNS3-3' and pRcCMV (P<0.01). The cloning efficiencies of transfected cells with pRcHCNS3-5', pRcHCNS3-3', pRcCMV and controls were 33%, 1.33%, 1.46%, 1.11% respectively. The level of phosphorylated MAPK in the cells with pRcHCNS3-5' was much higher than that in those with pRcHCNS3-3'and pRcCMV and normal cells (P<0.01). CONCLUSION: The results suggest that (1) QSG7701 cells are a better human liver cell line for investigating' the pathogenesis of HCV NS3 protein. (2) 5' region of the HCV genome segment encoding HCV NS3 is involved in cell growth and cell phenotype. (3) HCV NS3 N-terminal peptide may up-regulate the activation of MAPK, but not affect the expression of MAPK.
