As there is a strong interest in red-skinned pears,the molecular mechanism of anthocyanin regulation in red-skinned pears has been widely investigated;however,little is known about the molecular mechanism of anthocyan...As there is a strong interest in red-skinned pears,the molecular mechanism of anthocyanin regulation in red-skinned pears has been widely investigated;however,little is known about the molecular mechanism of anthocyanin regulation in red-fleshed pears due to limited availability of such germplasm,primarily found in European pears(Pyrus communis).In this study,based on transcriptomic analysis in red-fleshed and white-fleshed pears,we identified an ethylene response factor(ERF)from P.communis,PcERF5,of which expression level in fruit flesh was significantly correlated with anthocyanin content.We then verified the function of PcERF5 in regulating anthocyanin accumulation by genetic transformation in both pear skin and apple calli.PcERF5 regulated anthocyanin biosynthesis by different regulatory pathways.On the one hand,PcERF5 can activate the transcription of flavonoid biosynthetic genes(PcDFR,PcANS and PcUFGT)and two key transcription factors encoding genes PcMYB10 and PcMYB114.On the other hand,PcERF5 interacted with PcMYB10 to form the ERF5-MYB10 protein complex that enhanced the transcriptional activation of PcERF5 on its target genes.Our results suggested that PcERF5 functioned as a transcriptional activator in regulating anthocyanin biosynthesis,which provides new insights into the regulatory mechanism of anthocyanin biosynthesis.This new knowledge will provide guidance for molecular breeding of red-fleshed pear.展开更多
Cadmium(Cd)or excess copper(Cu)has a great impact in terms of toxicity on living organisms as it severely affects crop growth,yield and food security;thus,warranting appropriate measures for the remediation of Cd or C...Cadmium(Cd)or excess copper(Cu)has a great impact in terms of toxicity on living organisms as it severely affects crop growth,yield and food security;thus,warranting appropriate measures for the remediation of Cd or Cu polluted soils.Phytoextraction of heavy metal(HM)using tolerant plants along with organic chelators has gained global attention,and this study provided further insights into this issue.Pot experiments were performed to evaluate the effects of different types of chelators[ethylenediamine tetraacetic acid(EDTA),ethylenediamine disuccinic acid(EDDS)and citric acid(CA)]to improve the phytoextraction capacity of Ricinus communis L.for the metals Cd and Cu.Contaminated soil from a copper smelter was used in this study.A rhizon soil sampler was used to determine the metal concentrations in soil pore water.The results indicated that R.communis was an adequate candidate for chelator induced phytoextraction under the experimental conditions and that EDDS would be a good candidate chelator for the phytoextraction of Cu in soils.EDTA addition obviously improved the uptake of Cd and Cu in R.communis;however,it posed the greatest risk because the concentration of HMs in soil pore water was very high even after 40 days.Compared with EDTA and EDDS,CA had few effects on Cd or Cu uptake in R.communis.Linear relationships between the metal uptake in R.communis shoots and the maximum HM concentrations in soil pore water under HM,2.5,5,and 10 mmol·kg^(-1) treatments were typically observed.From the results of this study,it could be concluded that EDDS treatments played a promising role in increasing the uptake of Cd or Cu and reducing its phytotoxicity.EDDS application could be an effective approach for the phytoextraction of Cd or Cu from polluted soils by growing Ricinus communis L.展开更多
[Objective] The aim was to study on distribution of inorganic elements in kernel of Amygdalus communis L., providing reference for quality evaluation of A. communis L. species. [Method] Totally 26 species of inorganic...[Objective] The aim was to study on distribution of inorganic elements in kernel of Amygdalus communis L., providing reference for quality evaluation of A. communis L. species. [Method] Totally 26 species of inorganic elements in kernel, including Al, B, Be, Ca, Co, Cu, Fe, Mg, Mn, Mo, Na, Ni, P, Pb, Si, Sn, Sr, Ti, Zn, Cd, As, Se, V, Hg, Cr and K were measured with inductively coupled plasma emission spectrum （ICP-OES） and principal components analysis （PCA）. [Result] A. communis L. of different species and in different factories showed a similar curve in content of inorganic elements; absolute contents of the elements differed significantly. In addition, the accumulated variance contribution of five principle factors achieved as high as 84.371% and the variance contribution made by the first three factors accounted for 67.546%, proving that Fe, Ti, Pb, Na, Se, Cu, Mo, K, Zn, Ni, Ca and Sr were characteristic elements. [Conclusion] The method, which is brief, rapid and accurate, can be used for determination of inorganic elements in kernel of A. communis L., providing theoretical references for further development and utilization of A. communis L.展开更多
[Objective] The article studies the growth and Cu absorption of Ricinus communis L. callus under Cu stress. [Method] CuSO4.5H20 solutions with different Cu concentrations were added to callus subculture medium; callus...[Objective] The article studies the growth and Cu absorption of Ricinus communis L. callus under Cu stress. [Method] CuSO4.5H20 solutions with different Cu concentrations were added to callus subculture medium; callus was inoculated and Cu resistance index of callus was worked out. Cu content in callus was deter- mined with the method of Varian AA240FS. [Result] With the Cu concentration at 60 mg/L, the growth of callus was inhibited, its Cu resistance index was only 33.87%. With the Cu concentration at 40 mg/L, callus was faint yellow in color, and grew rapidly with its Cu resistance index at 61.29%. Such high level resistance could remain the same after six week after continuous subculture. In the 4t week of culture, Cu resistance index in treatments with Cu concentrations at 10, 20, 30, 40 mg/L was higher than that in the 3rd week, and the content of Cu in callus of the treatments was 0.33, 0.54, 1.16, 1.40 mg/g respectively. [Conclusion] Cu con- centration at 40 mg/L in culture medium can be the threshold for selecting Cu re- sistance R. communis callus.展开更多
Commelina communis L. growing over some new copper mining wastelands at Bijiashan, Tongling City of Anhui Province, China, was found to be a copper hyperaccumulator. Its copper concentrations were 2707-6159 (4439±...Commelina communis L. growing over some new copper mining wastelands at Bijiashan, Tongling City of Anhui Province, China, was found to be a copper hyperaccumulator. Its copper concentrations were 2707-6159 (4439±2434) mg kg-1, 369-831 (731±142) mg kg-1, and 429-587 (547±57) mg kg-1, respectively, in the roots, stems, and leaves. The soils supporting the growth of the species had a copper concentration ranging from 4620 to 5020 mg kg-1 and averaging 4835±262 mg kg-1, suggesting that the species could not only grow on heavily copper-contaminated soils but also accumulate extraordinarily high concentration of copper. Thus, it shows great potential in the phytoremediation of copper-contaminated soils,the restoration of mined land, geochemical prospecting, and the study of environmental pollution changes.展开更多
An efficient plant regeneration protocol was established for castor bean （Ricinus communis L.）, in which 0.3 mg L-1 thidiazuron （TDZ） induced shoot clusters and increased the number of adventitious shoots from hyp...An efficient plant regeneration protocol was established for castor bean （Ricinus communis L.）, in which 0.3 mg L-1 thidiazuron （TDZ） induced shoot clusters and increased the number of adventitious shoots from hypocotyl tissue. Our results showed that treatment under dark conditions significantly promoted the average number of shoots per explant to 37.36±4.54 （with a 6-d treatment）. Modified 1/2 Murashige and Skoog （MS） basal medium supplemented with 440 mg L-1 Ca2＋, 0.2 mg L-1 gibberellic acid and 0.1 mg L-1 TDZ significantly increased shoot elongation rates and lowered vitrification rates. Further- more, 1/2 MS media supplemented with 0.2 mg L-11-naphthaleneacetic acid induced a higher rooting rate compared with other culture conditions.展开更多
Among APETALA2 (AP2)-type plant specific transcription factor family, WRINKLED1 (WRI1), has appeared to be a master gene transcriptionally regulating a set of carbon metabolism- and fatty acid synthesis (FAS)-related ...Among APETALA2 (AP2)-type plant specific transcription factor family, WRINKLED1 (WRI1), has appeared to be a master gene transcriptionally regulating a set of carbon metabolism- and fatty acid synthesis (FAS)-related genes responsible for seed specific triacylglycerols (TAGs) storage in oil plants. B3 type transcription factors, such as ABI3 and FUS3, are known to be involved in seed development, such as seed storage protein synthesis and maturation. Based on the recent whole genome sequence data of castor bean (Ricinus communis L.), putative WRI1 homologs (RcWRI1, RcWRI2) specifically expressed in castor bean seed have been identified by comparing organ specific expression profiles among seed development-related transcription factors, seed storage specific genes (Ricin, RcOleosin) and a set of FAS genes including genes for sucrose synthase (RcSUS2), biotin carboxyl carrier protein (a subunit of acetyl-CoA carboxylase, RcBCCP2) and ketoacyl-acyl carrier protein synthase (RcKAS1). Immunoreactive signals with WRI1, FUS3 and ABI5-related polypeptides were also detected in seed specifically, consistent with the expression profiles of seed development-related genes. The WRI1 binding consensus sites, [CnTnG](n)(7)[CG], designated as the AW-box, were found at the promoter region of RcBCCP2 and RcKAS1. Thus, RcWRI1 possibly play a pivotal role in seed specific TAGs storage during seed development by directly activating FAS -related genes.展开更多
Light-harvesting chlorophyll a/b-binding （LHC） proteins are a group of nuclear-encoded thylakoid proteins that play a key role in plant photosynthesis and are widely involved in light harvesting, energy transfer to ...Light-harvesting chlorophyll a/b-binding （LHC） proteins are a group of nuclear-encoded thylakoid proteins that play a key role in plant photosynthesis and are widely involved in light harvesting, energy transfer to the reaction center, maintenance of thylakoid membrane structure, photoprotection and response to en- vironmental conditions, etc. Although/dw supergene family is well characterized in model plants such as Arabidopsis, rice and poplar, little information is available in castor bean （Ricinus communis L. ）. In this study, a genome-wide search was carried out for the first time to identify castor bean L/w genes and analyze the gene structures, biochemical properties, evolutionary relationships and expression characteristics based on the published data of castor bean genome and ESTs. According to the results, a total of 28 Rclhcs genes representing 13 gene families （ l_hca , l_hcb , Elip , Ohpl , Ohp2 , SEP1, SEP2 , SEP3 , SEP4 , SEP5 , PsbS , Rieske and FCII） and 25 subgene families were identified in castor bean genome; to be specific, 25 and 5 genes were found to have corresponding ESTs in NCBI and have al- ternative splicing isoforlns, respectively. These RcLhcs contain 0 to 9 introns and distribute on 26 of the 25 878 released scaffolds. All RcLhcs genes were found to be expressed in all examined tissues, i.e. leaf, flower, II/III stage endosperm, V/VI stage endosperm and seed, with the highest expression level in leaf tissue.展开更多
Castor(Ricinus communis L.)is one of ten oil crops in the world and has complex inflorescence styles.Generally,castor has three inflorescence types:single female inflorescence(SiFF),standard female inflorescence(StFF)...Castor(Ricinus communis L.)is one of ten oil crops in the world and has complex inflorescence styles.Generally,castor has three inflorescence types:single female inflorescence(SiFF),standard female inflorescence(StFF)and bisexual inflorescence(BF).StFF is realized as a restorer line and as a maintainer line,which was applied to castor hybrid breeding.However,the developmental mechanism of the three inflorescences is not clear.Therefore,we used proteomic techniques to analyze different inflorescence styles.A total of 72 diferentially abundant protein species(DAPs)were detected.These DAPs are primarily involved in carbon and energy metabolism and carbon fixation in the photosynthetic organism pathway.The results showed that DAPs are involved in photosynthesis to control the distribution of imported carbohydrates and exported photoassimilates and thus affect the inflorescence development of castor.In addition,these DAPs are also involved in cysteine and methionine metabolism.Quantitative real-time PCR(qRT-PCR)results demonstrated that the proteomics data collected in this study were reliable.Our findings indicate that the carbon cycle and amino acid metabolism influence the inflorescence development of castor.展开更多
We examined salt tolerance responsive genes in Pak-choi under salt stress and analyze their potential function. The LRNA differential display was used to screen the transcript derived fragments （TDFs） related to sal...We examined salt tolerance responsive genes in Pak-choi under salt stress and analyze their potential function. The LRNA differential display was used to screen the transcript derived fragments （TDFs） related to salinity tolerance in tolerant and Loderately tolerant Pak-choi germplasm. Seventy-eight primer combinations generated 101 differential eDNA fragments, which ere divided into 10 expression types. Seven cDNA sequences （GenBank accession Nos. DQ006915-DQ006921） obtained and ,~quenced were highly homologous to some known expression genes or the genes related to the signaling pathways in plants under ifferent abiotic stress.展开更多
In this study,the fibers of invasive species Agave americana L.and Ricinus communis L.were successfully used for the first time as new sources to produce cytocompatible and highly crystalline cellulose nanofibers.Cell...In this study,the fibers of invasive species Agave americana L.and Ricinus communis L.were successfully used for the first time as new sources to produce cytocompatible and highly crystalline cellulose nanofibers.Cellulose nanofibers were obtained by two methods,based on either alkaline or acid hydrolysis.The morphology,chemical composition,and crystallinity of the obtained materials were characterized by scanning electron microscopy(SEM)together with energy-dispersive X-ray spectroscopy(EDX),dynamic light scattering(DLS),X-ray diffraction(XRD),and Fourier transform infrared(FTIR)spectroscopy.The crystallinity indexes(CIs)of the cellulose nanofibers extracted from A.americana and R.communis were very high(94.1%and 92.7%,respectively).Biological studies evaluating the cytotoxic effects of the prepared cellulose nanofibers on human embryonic kidney 293 T(HEK293 T)cells were also performed.The nanofibers obtained using the two different extraction methods were all shown to be cytocompatible in the concentration range assayed(i.e.,0-500μg/mL).Our results showed that the nanocellulose extracted from A.americana and R.communis fibers has high potential as a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications.展开更多
Ricinus communis L. is a new copper hyperaccumulator growing on Tonglushan copper mine in Hubei Province, China. This study aimed to establish a suspension cell line of R. communis L. with stable and rapid growth for ...Ricinus communis L. is a new copper hyperaccumulator growing on Tonglushan copper mine in Hubei Province, China. This study aimed to establish a suspension cell line of R. communis L. with stable and rapid growth for further screening of heavy metal-resistant R. communis L. cells and breeding of hyperaccu- mulators. In this study, cell suspension culture conditions were optimized by using orthogonal experimental design with previously induced R. communis L. embyre- genic calluses as experimental materials, to establish the suspension cell line of R. communis L. Under the optimal conditions, growth curves of suspension cells and changes in pH of culture liquid were determined. The results showed that the optimal culture conditions for R. conmmnis L. suspension cell line were ： MS ＋ O. 5 rag/I, 6-BA ＋ O. 2 mg/L NAA ＋ 50 mg/L sucrose ＋ 350 mg/L casein hydrolysate as basic medium, with callus inoculation amount of 2.5 g/50 ml, dark culture at （26 ±2） ℃ with shaking at 110 r/min. Under these conditions, increment of fresh weight and dry weight ofR. commun/s L. suspension cells reached the maximum of 4.56 g/（50 ml 14 d） and 0.49 g/（50 ml 16 d）, respectively. Growth curves of R. communis L. suspension cells were basically in ＂S＂ shape. Each culture cycle lasted 16 d, and the rapid growth stage was from the 6th d to the 14th d. In a culture cycle, pH of the culture liquid declined first and then increased to the maximum and stabilized gradually.展开更多
The yield of castor is influenced by the type of inflorescence and the proportion of female flowers.However,there are few studies on the genetic mechanism involved in the development and differentiation of castor infl...The yield of castor is influenced by the type of inflorescence and the proportion of female flowers.However,there are few studies on the genetic mechanism involved in the development and differentiation of castor inflorescences.In this study,we performed transcriptomic analyses of three different phenotypes of inflorescences at the five-leaf stage.In comparison to the MI(complete pistil without willow leaves),290 and 89 differentially expressed genes(DEGs)were found in the SFI(complete pistil with willow leaves)and the BI(monoecious inflorescence),respectively.Among the DEGs,104 and 88 were upregulated in the SFI and BI,respectively,compared to the MI.In addition,186 DEGs and 1 DEG were downregulated in the SFI and BI compared to the MI.Moreover,we conducted GO and KEGG enrichment analyses of the DEGs.In comparison to the MI,the SFI and BI exhibited the enrichment of functional branches in DEGs,specifically in pollen wall assembly,pollen development,and cellular component assembly involved in morphogenesis.In our study,RADL5 showed low expression levels between SFI-vs.-MI types.In addition,we found that the expression of NAC in the SFI differed from that in MI and BI,and some genes related to hormonal signaling changed their expression levels during inflorescence differentiation.These results reveal the genetic mechanism of sex genotypes in castor,which will not only guide researchers in the breeding of castor but also provide a reference for genetic research on other flowering plants.展开更多
The Republic of Azerbaijan, is a country in the south Caucasus having continental influenced climate with warm summer and mild cold, dry winters. Relating to its climate this region has a rich and very interesting veg...The Republic of Azerbaijan, is a country in the south Caucasus having continental influenced climate with warm summer and mild cold, dry winters. Relating to its climate this region has a rich and very interesting vegetation cover. In presented article the vegetation communities with the presence of Juniperus communis L. species and subspecies have been described.展开更多
China is not only one of the origin centers of Pyrus L.,but also the earliest birthplace of Pyrus L.in the world.This paper reviews the evolution of Pyrus L.from the aspects of leaf edge morphology,inflorescence and f...China is not only one of the origin centers of Pyrus L.,but also the earliest birthplace of Pyrus L.in the world.This paper reviews the evolution of Pyrus L.from the aspects of leaf edge morphology,inflorescence and fruit type,and summarizes the research progress of classification and species distribution of Pyrus L.,which is of great significance for the protection,evaluation and utilization of germplasm resources.展开更多
在蓖麻(Ricinus communis L.)中克隆了PLC2N基因,运用生物信息学技术对PLC2N基因编码的蛋白质进行分析,以揭示其在蓖麻生长发育过程中的功能。结果表明,通过克隆蓖麻PLC2N基因,得到1785 bp的片段。PLC2N基因编码的蛋白质分子质量为67.98...在蓖麻(Ricinus communis L.)中克隆了PLC2N基因,运用生物信息学技术对PLC2N基因编码的蛋白质进行分析,以揭示其在蓖麻生长发育过程中的功能。结果表明,通过克隆蓖麻PLC2N基因,得到1785 bp的片段。PLC2N基因编码的蛋白质分子质量为67.98839 ku,等电点为6.16,有594个氨基酸残基,高度亲水,无跨膜区;二级结构中α-螺旋结构在蛋白质序列中占28.11%,共计167种氨基酸;延长链占19.02%,包含113种氨基酸;β转角结构占6.57%,含有39种氨基酸;无规则卷曲部分占总氨基酸数量的46.30%,氨基酸数量约275种。三级结构预测结果显示,该蛋白质包含了α-螺旋、β-转角以及无规则卷曲等关键结构,其中α-螺旋在蛋白质中占据核心地位。经同源性分析,发现蓖麻中的PLC2N基因与陆地棉这一物种之间存在较接近的亲缘关系。展开更多
蓖麻(Ricinus communis L.)作为重要的油料作物,其抗逆性研究对产业稳定发展至关重要,磷脂酶C(PLC)在植物逆境响应中发挥关键作用。以蓖麻Lm型雌性系aLmAB5品系为材料,采用PCR扩增技术克隆蓖麻PLC4基因(基因登录号:LOC8286461),并通过...蓖麻(Ricinus communis L.)作为重要的油料作物,其抗逆性研究对产业稳定发展至关重要,磷脂酶C(PLC)在植物逆境响应中发挥关键作用。以蓖麻Lm型雌性系aLmAB5品系为材料,采用PCR扩增技术克隆蓖麻PLC4基因(基因登录号:LOC8286461),并通过生物信息学方法系统分析其编码蛋白质特性。结果显示,成功克隆得到长度为1770 bp的PLC4基因,测序验证与参考序列同源性达100%;其编码蛋白质由589个氨基酸组成,分子式为C_(3008)H_(4726)N_(812)O_(875)S_(31),分子质量为67260 g/mol,等电点7.61,属于亲水性不稳定蛋白;该蛋白含PLCXc、PLCYc和C23个高度保守结构域,二级结构以无规卷曲(48.56%)和α-螺旋(26.99%)为主,三级结构模型可信度高(GMQE=0.85),明确属于磷脂酰肌醇磷酸酶C基因家族;系统进化树分析表明,其与陆地棉PLC蛋白亲缘关系最近。研究结论为解析PLC4基因在蓖麻抗逆性中的作用机制提供理论依据,也为蓖麻抗逆分子育种提供候选基因资源。展开更多
基金funded by the National Natural Science Foundation of China(31820103012)the earmarked fund for China Agriculture Research System(CARS-28)the earmarked fund for Jiangsu Agricultural Industry Technology System,China(JATS[2022]454).
文摘As there is a strong interest in red-skinned pears,the molecular mechanism of anthocyanin regulation in red-skinned pears has been widely investigated;however,little is known about the molecular mechanism of anthocyanin regulation in red-fleshed pears due to limited availability of such germplasm,primarily found in European pears(Pyrus communis).In this study,based on transcriptomic analysis in red-fleshed and white-fleshed pears,we identified an ethylene response factor(ERF)from P.communis,PcERF5,of which expression level in fruit flesh was significantly correlated with anthocyanin content.We then verified the function of PcERF5 in regulating anthocyanin accumulation by genetic transformation in both pear skin and apple calli.PcERF5 regulated anthocyanin biosynthesis by different regulatory pathways.On the one hand,PcERF5 can activate the transcription of flavonoid biosynthetic genes(PcDFR,PcANS and PcUFGT)and two key transcription factors encoding genes PcMYB10 and PcMYB114.On the other hand,PcERF5 interacted with PcMYB10 to form the ERF5-MYB10 protein complex that enhanced the transcriptional activation of PcERF5 on its target genes.Our results suggested that PcERF5 functioned as a transcriptional activator in regulating anthocyanin biosynthesis,which provides new insights into the regulatory mechanism of anthocyanin biosynthesis.This new knowledge will provide guidance for molecular breeding of red-fleshed pear.
基金Supported by the Joint Funds of the National Natural Science Foundation of China(U2340219)。
文摘Cadmium(Cd)or excess copper(Cu)has a great impact in terms of toxicity on living organisms as it severely affects crop growth,yield and food security;thus,warranting appropriate measures for the remediation of Cd or Cu polluted soils.Phytoextraction of heavy metal(HM)using tolerant plants along with organic chelators has gained global attention,and this study provided further insights into this issue.Pot experiments were performed to evaluate the effects of different types of chelators[ethylenediamine tetraacetic acid(EDTA),ethylenediamine disuccinic acid(EDDS)and citric acid(CA)]to improve the phytoextraction capacity of Ricinus communis L.for the metals Cd and Cu.Contaminated soil from a copper smelter was used in this study.A rhizon soil sampler was used to determine the metal concentrations in soil pore water.The results indicated that R.communis was an adequate candidate for chelator induced phytoextraction under the experimental conditions and that EDDS would be a good candidate chelator for the phytoextraction of Cu in soils.EDTA addition obviously improved the uptake of Cd and Cu in R.communis;however,it posed the greatest risk because the concentration of HMs in soil pore water was very high even after 40 days.Compared with EDTA and EDDS,CA had few effects on Cd or Cu uptake in R.communis.Linear relationships between the metal uptake in R.communis shoots and the maximum HM concentrations in soil pore water under HM,2.5,5,and 10 mmol·kg^(-1) treatments were typically observed.From the results of this study,it could be concluded that EDDS treatments played a promising role in increasing the uptake of Cd or Cu and reducing its phytotoxicity.EDDS application could be an effective approach for the phytoextraction of Cd or Cu from polluted soils by growing Ricinus communis L.
基金Supported by the Pillar Program of Ministry of Science and Technology of the People's Republic of China (2012BAI27B07)the Fundamental Research Funds for the Central Universities (11NZYTH02)+1 种基金Sichuan Key Technology Research and Development Program (2011SZ0233)Academic Technology for Excellent Youth Follow-up Plan in Sichuan (2011JQ0051)~~
文摘[Objective] The aim was to study on distribution of inorganic elements in kernel of Amygdalus communis L., providing reference for quality evaluation of A. communis L. species. [Method] Totally 26 species of inorganic elements in kernel, including Al, B, Be, Ca, Co, Cu, Fe, Mg, Mn, Mo, Na, Ni, P, Pb, Si, Sn, Sr, Ti, Zn, Cd, As, Se, V, Hg, Cr and K were measured with inductively coupled plasma emission spectrum （ICP-OES） and principal components analysis （PCA）. [Result] A. communis L. of different species and in different factories showed a similar curve in content of inorganic elements; absolute contents of the elements differed significantly. In addition, the accumulated variance contribution of five principle factors achieved as high as 84.371% and the variance contribution made by the first three factors accounted for 67.546%, proving that Fe, Ti, Pb, Na, Se, Cu, Mo, K, Zn, Ni, Ca and Sr were characteristic elements. [Conclusion] The method, which is brief, rapid and accurate, can be used for determination of inorganic elements in kernel of A. communis L., providing theoretical references for further development and utilization of A. communis L.
基金Supported by General Program of National Natural Science Foundation of China(41371470)Key Program of Hubei Natural Science Foundation(2011CDA037)Students’Sci-Tech Innovation Program of Hubei Polytechnic University(2012cx23)~~
文摘[Objective] The article studies the growth and Cu absorption of Ricinus communis L. callus under Cu stress. [Method] CuSO4.5H20 solutions with different Cu concentrations were added to callus subculture medium; callus was inoculated and Cu resistance index of callus was worked out. Cu content in callus was deter- mined with the method of Varian AA240FS. [Result] With the Cu concentration at 60 mg/L, the growth of callus was inhibited, its Cu resistance index was only 33.87%. With the Cu concentration at 40 mg/L, callus was faint yellow in color, and grew rapidly with its Cu resistance index at 61.29%. Such high level resistance could remain the same after six week after continuous subculture. In the 4t week of culture, Cu resistance index in treatments with Cu concentrations at 10, 20, 30, 40 mg/L was higher than that in the 3rd week, and the content of Cu in callus of the treatments was 0.33, 0.54, 1.16, 1.40 mg/g respectively. [Conclusion] Cu con- centration at 40 mg/L in culture medium can be the threshold for selecting Cu re- sistance R. communis callus.
文摘Commelina communis L. growing over some new copper mining wastelands at Bijiashan, Tongling City of Anhui Province, China, was found to be a copper hyperaccumulator. Its copper concentrations were 2707-6159 (4439±2434) mg kg-1, 369-831 (731±142) mg kg-1, and 429-587 (547±57) mg kg-1, respectively, in the roots, stems, and leaves. The soils supporting the growth of the species had a copper concentration ranging from 4620 to 5020 mg kg-1 and averaging 4835±262 mg kg-1, suggesting that the species could not only grow on heavily copper-contaminated soils but also accumulate extraordinarily high concentration of copper. Thus, it shows great potential in the phytoremediation of copper-contaminated soils,the restoration of mined land, geochemical prospecting, and the study of environmental pollution changes.
基金supported by the National Natural Science Foundation of China (31260336 and 31460353)
文摘An efficient plant regeneration protocol was established for castor bean （Ricinus communis L.）, in which 0.3 mg L-1 thidiazuron （TDZ） induced shoot clusters and increased the number of adventitious shoots from hypocotyl tissue. Our results showed that treatment under dark conditions significantly promoted the average number of shoots per explant to 37.36±4.54 （with a 6-d treatment）. Modified 1/2 Murashige and Skoog （MS） basal medium supplemented with 440 mg L-1 Ca2＋, 0.2 mg L-1 gibberellic acid and 0.1 mg L-1 TDZ significantly increased shoot elongation rates and lowered vitrification rates. Further- more, 1/2 MS media supplemented with 0.2 mg L-11-naphthaleneacetic acid induced a higher rooting rate compared with other culture conditions.
文摘Among APETALA2 (AP2)-type plant specific transcription factor family, WRINKLED1 (WRI1), has appeared to be a master gene transcriptionally regulating a set of carbon metabolism- and fatty acid synthesis (FAS)-related genes responsible for seed specific triacylglycerols (TAGs) storage in oil plants. B3 type transcription factors, such as ABI3 and FUS3, are known to be involved in seed development, such as seed storage protein synthesis and maturation. Based on the recent whole genome sequence data of castor bean (Ricinus communis L.), putative WRI1 homologs (RcWRI1, RcWRI2) specifically expressed in castor bean seed have been identified by comparing organ specific expression profiles among seed development-related transcription factors, seed storage specific genes (Ricin, RcOleosin) and a set of FAS genes including genes for sucrose synthase (RcSUS2), biotin carboxyl carrier protein (a subunit of acetyl-CoA carboxylase, RcBCCP2) and ketoacyl-acyl carrier protein synthase (RcKAS1). Immunoreactive signals with WRI1, FUS3 and ABI5-related polypeptides were also detected in seed specifically, consistent with the expression profiles of seed development-related genes. The WRI1 binding consensus sites, [CnTnG](n)(7)[CG], designated as the AW-box, were found at the promoter region of RcBCCP2 and RcKAS1. Thus, RcWRI1 possibly play a pivotal role in seed specific TAGs storage during seed development by directly activating FAS -related genes.
基金Supported by National Natural Science Foundation of China(31100460)Natural Science Foundation of Hainan Province(312026)Fundamental Research Fund for the Rubber Research Institute in Chinese Academy of Tropical Agricultural Sciences(1630022011014)
文摘Light-harvesting chlorophyll a/b-binding （LHC） proteins are a group of nuclear-encoded thylakoid proteins that play a key role in plant photosynthesis and are widely involved in light harvesting, energy transfer to the reaction center, maintenance of thylakoid membrane structure, photoprotection and response to en- vironmental conditions, etc. Although/dw supergene family is well characterized in model plants such as Arabidopsis, rice and poplar, little information is available in castor bean （Ricinus communis L. ）. In this study, a genome-wide search was carried out for the first time to identify castor bean L/w genes and analyze the gene structures, biochemical properties, evolutionary relationships and expression characteristics based on the published data of castor bean genome and ESTs. According to the results, a total of 28 Rclhcs genes representing 13 gene families （ l_hca , l_hcb , Elip , Ohpl , Ohp2 , SEP1, SEP2 , SEP3 , SEP4 , SEP5 , PsbS , Rieske and FCII） and 25 subgene families were identified in castor bean genome; to be specific, 25 and 5 genes were found to have corresponding ESTs in NCBI and have al- ternative splicing isoforlns, respectively. These RcLhcs contain 0 to 9 introns and distribute on 26 of the 25 878 released scaffolds. All RcLhcs genes were found to be expressed in all examined tissues, i.e. leaf, flower, II/III stage endosperm, V/VI stage endosperm and seed, with the highest expression level in leaf tissue.
基金This study was supported by the National Natural Science Foundation of China(31860071)Research and Reform Practice Project in New Agricultural Sciences of the Ministry of Education in 2020(2020114)+7 种基金Natural Science Foundation of Inner Mongolia Autonomous Region(2021MS03008)Inner Mongolia Autonomous Region Grassland Talents Innovation Team—Castor Molecular Breeding Research Innovative Talent Team Rolling Support Project(2022)Higher Education Teaching Reform Research Project of National Ethnic Affairs Commission in 2021(21082)Fundamental Research Funds in Higher Education Institutions of Inner Mongolia in 2022(237)Autonomous Region Basic Scientific Reasearch Business Fee Projest of Inner Mongolia MinZu University in 2023(225,227,244)Inner Mongolia Autonomous Region Castor Industry Collaborative Innovation Center Construction Project(MDK2021011,MDK2022014)Open Fund Project in State Key Laboratory of Castor Breeding of China’s National Ethnic Affairs Commission(MDK2021008)Science and Technology Research Project of Jilin Provincial Department of Education(JJKH20220010KJ).
文摘Castor(Ricinus communis L.)is one of ten oil crops in the world and has complex inflorescence styles.Generally,castor has three inflorescence types:single female inflorescence(SiFF),standard female inflorescence(StFF)and bisexual inflorescence(BF).StFF is realized as a restorer line and as a maintainer line,which was applied to castor hybrid breeding.However,the developmental mechanism of the three inflorescences is not clear.Therefore,we used proteomic techniques to analyze different inflorescence styles.A total of 72 diferentially abundant protein species(DAPs)were detected.These DAPs are primarily involved in carbon and energy metabolism and carbon fixation in the photosynthetic organism pathway.The results showed that DAPs are involved in photosynthesis to control the distribution of imported carbohydrates and exported photoassimilates and thus affect the inflorescence development of castor.In addition,these DAPs are also involved in cysteine and methionine metabolism.Quantitative real-time PCR(qRT-PCR)results demonstrated that the proteomics data collected in this study were reliable.Our findings indicate that the carbon cycle and amino acid metabolism influence the inflorescence development of castor.
基金Project supported by the National "the Tenth Five-Year-Plan" Key Program (No. 2004BA525B08)China and the Key Laboratory of Vegetable Genetics and Physiology, Ministry of Agriculture, China
文摘We examined salt tolerance responsive genes in Pak-choi under salt stress and analyze their potential function. The LRNA differential display was used to screen the transcript derived fragments （TDFs） related to salinity tolerance in tolerant and Loderately tolerant Pak-choi germplasm. Seventy-eight primer combinations generated 101 differential eDNA fragments, which ere divided into 10 expression types. Seven cDNA sequences （GenBank accession Nos. DQ006915-DQ006921） obtained and ,~quenced were highly homologous to some known expression genes or the genes related to the signaling pathways in plants under ifferent abiotic stress.
基金the Programa de Cooperación Territorial INTERREG V-A MAC 2014-2020 and Inv2Mac Project(MAC/4.6d/229)the partial support of FCT-Funda??o para a Ciência e a Tecnologia(Base Fund UIDB/00674/2020)+1 种基金ARDITI-Agência Regional para o Desenvolvimento da Investiga??o Tecnologia e Inova??o supported the study through the project M1420-01-0145FEDER-000005-CQM+(Madeira 14-20 Program)the Postdoc Grant(M1420-09-5369-FSE-000001,002458/2015/132)for Carla S.ALVES is also acknowledged。
文摘In this study,the fibers of invasive species Agave americana L.and Ricinus communis L.were successfully used for the first time as new sources to produce cytocompatible and highly crystalline cellulose nanofibers.Cellulose nanofibers were obtained by two methods,based on either alkaline or acid hydrolysis.The morphology,chemical composition,and crystallinity of the obtained materials were characterized by scanning electron microscopy(SEM)together with energy-dispersive X-ray spectroscopy(EDX),dynamic light scattering(DLS),X-ray diffraction(XRD),and Fourier transform infrared(FTIR)spectroscopy.The crystallinity indexes(CIs)of the cellulose nanofibers extracted from A.americana and R.communis were very high(94.1%and 92.7%,respectively).Biological studies evaluating the cytotoxic effects of the prepared cellulose nanofibers on human embryonic kidney 293 T(HEK293 T)cells were also performed.The nanofibers obtained using the two different extraction methods were all shown to be cytocompatible in the concentration range assayed(i.e.,0-500μg/mL).Our results showed that the nanocellulose extracted from A.americana and R.communis fibers has high potential as a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications.
基金Supported by General Project of National Natural Science Foundation of China(41371470)Key Program of Natural Science Foundation of Hubei Province(2011CDA037)Science and Technology Planning Project of Huangshi City(2010A1019-2)
文摘Ricinus communis L. is a new copper hyperaccumulator growing on Tonglushan copper mine in Hubei Province, China. This study aimed to establish a suspension cell line of R. communis L. with stable and rapid growth for further screening of heavy metal-resistant R. communis L. cells and breeding of hyperaccu- mulators. In this study, cell suspension culture conditions were optimized by using orthogonal experimental design with previously induced R. communis L. embyre- genic calluses as experimental materials, to establish the suspension cell line of R. communis L. Under the optimal conditions, growth curves of suspension cells and changes in pH of culture liquid were determined. The results showed that the optimal culture conditions for R. conmmnis L. suspension cell line were ： MS ＋ O. 5 rag/I, 6-BA ＋ O. 2 mg/L NAA ＋ 50 mg/L sucrose ＋ 350 mg/L casein hydrolysate as basic medium, with callus inoculation amount of 2.5 g/50 ml, dark culture at （26 ±2） ℃ with shaking at 110 r/min. Under these conditions, increment of fresh weight and dry weight ofR. commun/s L. suspension cells reached the maximum of 4.56 g/（50 ml 14 d） and 0.49 g/（50 ml 16 d）, respectively. Growth curves of R. communis L. suspension cells were basically in ＂S＂ shape. Each culture cycle lasted 16 d, and the rapid growth stage was from the 6th d to the 14th d. In a culture cycle, pH of the culture liquid declined first and then increased to the maximum and stabilized gradually.
基金the following agencies:the Natural Science Foundation of Jilin Province(YDZJ202201ZYTS453)the Scientific Research Project of the Jilin Provincial Department of Education(JJKH20220010KJ)+6 种基金the Program for Innovative Research Team of Baicheng Normal University,the National Natural Science Foundation of China(31860071)the Inner Mongolia Autonomous Region Natural Science Foundation Project(2021MS03008)the Inner Mongolia Autonomous Region Grassland Talent Innovation Team(2022)the 2022 Basic Scientific Research Business Cost Project of Universities Directly under the Autonomous Region(237)the Open Fund Project of Inner Mongolia Castor Industry Collaborative Innovation Center(MDK2021011,MDK2022014,MDK2022008,MDK2021008,MDK2022009,MDK2023003)Fundamental Research Funds for Universities Directly under the Autonomous Region in 2023 of Inner Mongolia University for Nationalities(225,227,243,244)New Agricultural Science Research and Reform Practice Project of the Ministry of Education(2020114)。
文摘The yield of castor is influenced by the type of inflorescence and the proportion of female flowers.However,there are few studies on the genetic mechanism involved in the development and differentiation of castor inflorescences.In this study,we performed transcriptomic analyses of three different phenotypes of inflorescences at the five-leaf stage.In comparison to the MI(complete pistil without willow leaves),290 and 89 differentially expressed genes(DEGs)were found in the SFI(complete pistil with willow leaves)and the BI(monoecious inflorescence),respectively.Among the DEGs,104 and 88 were upregulated in the SFI and BI,respectively,compared to the MI.In addition,186 DEGs and 1 DEG were downregulated in the SFI and BI compared to the MI.Moreover,we conducted GO and KEGG enrichment analyses of the DEGs.In comparison to the MI,the SFI and BI exhibited the enrichment of functional branches in DEGs,specifically in pollen wall assembly,pollen development,and cellular component assembly involved in morphogenesis.In our study,RADL5 showed low expression levels between SFI-vs.-MI types.In addition,we found that the expression of NAC in the SFI differed from that in MI and BI,and some genes related to hormonal signaling changed their expression levels during inflorescence differentiation.These results reveal the genetic mechanism of sex genotypes in castor,which will not only guide researchers in the breeding of castor but also provide a reference for genetic research on other flowering plants.
文摘The Republic of Azerbaijan, is a country in the south Caucasus having continental influenced climate with warm summer and mild cold, dry winters. Relating to its climate this region has a rich and very interesting vegetation cover. In presented article the vegetation communities with the presence of Juniperus communis L. species and subspecies have been described.
基金Supported by the Natural Science Foundation of Henan Provincial Department of Science and Technology(222300420508)Henan Provincial Key Science and Technology Research Project(202102310478)+1 种基金the Ph D Research Fund of Pingdingshan University(PXY-BSQD-2012009)Training Program for Young Backbone Teachers in Higher Education Institutions in Henan Province(2021GGJS147)。
文摘China is not only one of the origin centers of Pyrus L.,but also the earliest birthplace of Pyrus L.in the world.This paper reviews the evolution of Pyrus L.from the aspects of leaf edge morphology,inflorescence and fruit type,and summarizes the research progress of classification and species distribution of Pyrus L.,which is of great significance for the protection,evaluation and utilization of germplasm resources.
文摘在蓖麻(Ricinus communis L.)中克隆了PLC2N基因,运用生物信息学技术对PLC2N基因编码的蛋白质进行分析,以揭示其在蓖麻生长发育过程中的功能。结果表明,通过克隆蓖麻PLC2N基因,得到1785 bp的片段。PLC2N基因编码的蛋白质分子质量为67.98839 ku,等电点为6.16,有594个氨基酸残基,高度亲水,无跨膜区;二级结构中α-螺旋结构在蛋白质序列中占28.11%,共计167种氨基酸;延长链占19.02%,包含113种氨基酸;β转角结构占6.57%,含有39种氨基酸;无规则卷曲部分占总氨基酸数量的46.30%,氨基酸数量约275种。三级结构预测结果显示,该蛋白质包含了α-螺旋、β-转角以及无规则卷曲等关键结构,其中α-螺旋在蛋白质中占据核心地位。经同源性分析,发现蓖麻中的PLC2N基因与陆地棉这一物种之间存在较接近的亲缘关系。
文摘蓖麻(Ricinus communis L.)作为重要的油料作物,其抗逆性研究对产业稳定发展至关重要,磷脂酶C(PLC)在植物逆境响应中发挥关键作用。以蓖麻Lm型雌性系aLmAB5品系为材料,采用PCR扩增技术克隆蓖麻PLC4基因(基因登录号:LOC8286461),并通过生物信息学方法系统分析其编码蛋白质特性。结果显示,成功克隆得到长度为1770 bp的PLC4基因,测序验证与参考序列同源性达100%;其编码蛋白质由589个氨基酸组成,分子式为C_(3008)H_(4726)N_(812)O_(875)S_(31),分子质量为67260 g/mol,等电点7.61,属于亲水性不稳定蛋白;该蛋白含PLCXc、PLCYc和C23个高度保守结构域,二级结构以无规卷曲(48.56%)和α-螺旋(26.99%)为主,三级结构模型可信度高(GMQE=0.85),明确属于磷脂酰肌醇磷酸酶C基因家族;系统进化树分析表明,其与陆地棉PLC蛋白亲缘关系最近。研究结论为解析PLC4基因在蓖麻抗逆性中的作用机制提供理论依据,也为蓖麻抗逆分子育种提供候选基因资源。
