Spruce budworm （Choristoneura fumiferana （Clemens）） is one of the most serious forest insect pests in North America. Laboratory bioassays were performed to determine the lethal doses and lethal time of pyrethrins ...Spruce budworm （Choristoneura fumiferana （Clemens）） is one of the most serious forest insect pests in North America. Laboratory bioassays were performed to determine the lethal doses and lethal time of pyrethrins （a botanical insecticide） on 4th instar larvae of spruce bud-worm using larval dip assay. Results show that the LT50 values（time of 50% larval mortality）for spruce budworm at the pyrethrins concentra-tions of 12.5, 25, 50, 100, and 200 μg·L-1 were 94.78, 45.54, 20.36, 14.39 and 11.37 h, respectively. The percentage of cumulative mortality at the pyrethrins concentrations of 12.5, 25, 50, 100, 200 μg·L-1 was approxi-mately 50%, 67%, 93%, 100% and 100% within 120 h, respectively. The LC50 value （concentration of 50% larval mortality） for the 4th instar lar-vae was 16.1μg·L-1. Thus, larval mortality of spruce budworm increased in a concentration-dependent manner, and lethal time decreased with increasing pyrethin concentrations. These findings suggest that pyrethrins have a potential in controlling spruce budworm populations.展开更多
Four undescribed pyrethrins C-F(1-4)as well as four known pyrethrins(5-8)were isolated from seeds of Pyrethrum cinerariifolium Trev.The structures of compounds 1-4 were elucidated by UV,HRESIMS,and NMR(^(1)H and ^(13)...Four undescribed pyrethrins C-F(1-4)as well as four known pyrethrins(5-8)were isolated from seeds of Pyrethrum cinerariifolium Trev.The structures of compounds 1-4 were elucidated by UV,HRESIMS,and NMR(^(1)H and ^(13)C NMR,^(1)H-^(1)H COSY,HSQC,HMBC and ROESY),among which the stereostructure of compound 4 was determined by calcu-lated ECD.Furthermore,compounds 1-4 were evaluated for their aphidicidal activities.The insecticidal assay results showed that 1-4 exhibited moderate aphidicidal activities at the concentration of 0.1 mg/mL with the 24 h mortality rates ranging from 10.58 to 52.98%.Among them,pyrethrin D(2)showed the highest aphidicidal activity,with the 24 h mortality rate of 52.98%,which was slightly lower than the positive control(pyrethrin II,83.52%).展开更多
Using bio-chassis technology to realize the environmental protection and low energy consumption production of biological pesticides is the main goal of the future development of synthetic biology technology. Pyrethroi...Using bio-chassis technology to realize the environmental protection and low energy consumption production of biological pesticides is the main goal of the future development of synthetic biology technology. Pyrethroid is a kind of pure natural effective insecticide derived from pyrethrum, an herbal plant of Compositae. It has broad-spectrum and strong insecticidal and de-worming effects, and has little harm to mammals and no environmental harm. It is one of the best substitutes for biological insecticides, and has a wider application prospect. Natural pyrethrins, on the other hand, consist of six main components which are formed by condensation of two heteromorphic terpenoid acid ligands and three alcohol ligands which are the main raw materials of jasmonic acid. In this paper, the whole research and development process of pyrethrins is summarized. By using the analyzed plant metabolic synthesis methods and microbial systems to prepare pyrethrins on a large scale, it can provide an example for the scientific research foundation and application practice of preparing green biopesticides by synthetic biology methods.展开更多
Fipronil is an insecticide with low residual activity and high efficacy at low concentrations.Due to its widespread use and long half-life,fipronil can persist during wastewater treatment and is found even in surface ...Fipronil is an insecticide with low residual activity and high efficacy at low concentrations.Due to its widespread use and long half-life,fipronil can persist during wastewater treatment and is found even in surface waters.When released into the environment,it can accumulate over time and can lead to concentrations in soil and plant tissues that are harmful to both target and non-target organisms.As the reuse of treated wastewater and sewage sludge presents challenges and opportunities for farmers,this study investigates the effects of irrigation with environmentally relevant doses of fipronil on zucchini plants and their main insect pest,Aphis gossypii.Traces of fipronil were found in the zucchini leaves,but not in the flowers,after 35-days of cumulative exposure.A decrease in nymph-to-adult survival and a dose-dependent reduction in the fecundity of A.gossypii feeding on contaminated host plants was observed.Also,aphids feeding on contaminated host plants exhibited the same mortality rate as the control group when exposed to an acute dose of fipronil.However,when natural pyrethrins were used,there was a significant increase in resistance to this insecticide.Our results demonstrate the potential for fipronil to accumulate in plant tissues and highlight the risk of changes in insecticide susceptibility in insect pests.This suggests a need for a holistic approach to the complex dynamics of wastewater reuse in agriculture.展开更多
基金This research was supported by Natural Resources Canada, Canadian Forest Service. Project No. CFSCB2005-09.
文摘Spruce budworm （Choristoneura fumiferana （Clemens）） is one of the most serious forest insect pests in North America. Laboratory bioassays were performed to determine the lethal doses and lethal time of pyrethrins （a botanical insecticide） on 4th instar larvae of spruce bud-worm using larval dip assay. Results show that the LT50 values（time of 50% larval mortality）for spruce budworm at the pyrethrins concentra-tions of 12.5, 25, 50, 100, and 200 μg·L-1 were 94.78, 45.54, 20.36, 14.39 and 11.37 h, respectively. The percentage of cumulative mortality at the pyrethrins concentrations of 12.5, 25, 50, 100, 200 μg·L-1 was approxi-mately 50%, 67%, 93%, 100% and 100% within 120 h, respectively. The LC50 value （concentration of 50% larval mortality） for the 4th instar lar-vae was 16.1μg·L-1. Thus, larval mortality of spruce budworm increased in a concentration-dependent manner, and lethal time decreased with increasing pyrethin concentrations. These findings suggest that pyrethrins have a potential in controlling spruce budworm populations.
基金the Key Research and Development Program of Yunnan Province,China(202003AD 150006)the Cooperation Project with DR PLANT Company(2023).
文摘Four undescribed pyrethrins C-F(1-4)as well as four known pyrethrins(5-8)were isolated from seeds of Pyrethrum cinerariifolium Trev.The structures of compounds 1-4 were elucidated by UV,HRESIMS,and NMR(^(1)H and ^(13)C NMR,^(1)H-^(1)H COSY,HSQC,HMBC and ROESY),among which the stereostructure of compound 4 was determined by calcu-lated ECD.Furthermore,compounds 1-4 were evaluated for their aphidicidal activities.The insecticidal assay results showed that 1-4 exhibited moderate aphidicidal activities at the concentration of 0.1 mg/mL with the 24 h mortality rates ranging from 10.58 to 52.98%.Among them,pyrethrin D(2)showed the highest aphidicidal activity,with the 24 h mortality rate of 52.98%,which was slightly lower than the positive control(pyrethrin II,83.52%).
文摘Using bio-chassis technology to realize the environmental protection and low energy consumption production of biological pesticides is the main goal of the future development of synthetic biology technology. Pyrethroid is a kind of pure natural effective insecticide derived from pyrethrum, an herbal plant of Compositae. It has broad-spectrum and strong insecticidal and de-worming effects, and has little harm to mammals and no environmental harm. It is one of the best substitutes for biological insecticides, and has a wider application prospect. Natural pyrethrins, on the other hand, consist of six main components which are formed by condensation of two heteromorphic terpenoid acid ligands and three alcohol ligands which are the main raw materials of jasmonic acid. In this paper, the whole research and development process of pyrethrins is summarized. By using the analyzed plant metabolic synthesis methods and microbial systems to prepare pyrethrins on a large scale, it can provide an example for the scientific research foundation and application practice of preparing green biopesticides by synthetic biology methods.
基金funding from MUR under the umbrella of the PRIMA-Partnership for Research&Innovation in the Mediterranean Area through the research project SAFE"Sustainable water reuse practices improving safety in agriculture,food and environment"support from the CERCA Institute through the CERCAGINYS programme,funded by the Spanish Ministry of Science and Innovationsupport from the Economy and Knowledge Department of the Catalan Government through a Consolidated Research Groups(ICRA-ENV-2021 SGR 01282 and ICRA-TECH 2021 SGR 01283)。
文摘Fipronil is an insecticide with low residual activity and high efficacy at low concentrations.Due to its widespread use and long half-life,fipronil can persist during wastewater treatment and is found even in surface waters.When released into the environment,it can accumulate over time and can lead to concentrations in soil and plant tissues that are harmful to both target and non-target organisms.As the reuse of treated wastewater and sewage sludge presents challenges and opportunities for farmers,this study investigates the effects of irrigation with environmentally relevant doses of fipronil on zucchini plants and their main insect pest,Aphis gossypii.Traces of fipronil were found in the zucchini leaves,but not in the flowers,after 35-days of cumulative exposure.A decrease in nymph-to-adult survival and a dose-dependent reduction in the fecundity of A.gossypii feeding on contaminated host plants was observed.Also,aphids feeding on contaminated host plants exhibited the same mortality rate as the control group when exposed to an acute dose of fipronil.However,when natural pyrethrins were used,there was a significant increase in resistance to this insecticide.Our results demonstrate the potential for fipronil to accumulate in plant tissues and highlight the risk of changes in insecticide susceptibility in insect pests.This suggests a need for a holistic approach to the complex dynamics of wastewater reuse in agriculture.
