[Objectives]This study aimed to quantitatively investigate the dynamic patterns of spore powder of Metarhizium anisopliae and Beauveria bassiana carried on the body surface of Pyemotes zhonghuajia,providing a theoreti...[Objectives]This study aimed to quantitatively investigate the dynamic patterns of spore powder of Metarhizium anisopliae and Beauveria bassiana carried on the body surface of Pyemotes zhonghuajia,providing a theoretical basis for optimizing synergistic biological control strategies utilizing natural enemies and pathogens.[Methods]Under laboratory conditions,the spore load on mites after crawling on a spore-powder-coated surface for different durations(0-4 h),and the spore shedding after crawling on a clean surface for different durations(0-4 h)post-loading were measured.[Results]The spore load of mites for both fungi increased with crawling time,but exhibited distinct kinetic patterns.The load of M.anisopliae spores peaked at 2 h(6.86×104 spores/mite)and then decreased significantly;whereas the load of B.bassiana spores continued to increase to a maximum(12.83×104 spores/mite)within 4 h,with the rate of increase slowing significantly after 2 h.During the shedding phase,the number of both types of spores decreased with crawling time.After 4 h,the residual amount of B.bassiana spores(3.18×104 spores/mite)was significantly higher than that of M.anisopliae(2.51×104 spores/mite).[Conclusions]The process of P.zhonghuajia carrying entomopathogenic fungal spores exhibits significant temporal dynamics and species specificity.The findings identify key time points for spore loading and shedding,providing crucial parameters for determining the optimal pre-release"loading"duration and assessing the mites sustained dispersal capacity in the field,which holds significant importance for advancing the application of synergistic biological control technology.展开更多
[ Objective] The paper was to study the parasitic ability and field control effect of Pyemotes sp. against oriental fruit moth （Grapholitha molesta Bussck） which caused damage on Photinia. [ Method] The indoor infec...[ Objective] The paper was to study the parasitic ability and field control effect of Pyemotes sp. against oriental fruit moth （Grapholitha molesta Bussck） which caused damage on Photinia. [ Method] The indoor infection test and field control test of Pyemotes sp. against oriental fruit moth was carried out. The parasitic ability and activity characteristic of Pyemotes sp. were observed, and the effect of environmental factors such as humidity and temperature on the para- sitic ability of Pyemotes sp. was studied. [Result] Pyemotes sp. had good control effect on oriental fruit moth under the proper conditions; the host searching abili- ties of Pyemotes sp. were different at different temperature and humidity conditions, humidity had large impact on the activity of Pyemotes sp., while temperature had large impact on the individual activity. [ Conclusion] The result showed that Pyemotes sp. had effective control effort on oriental fruit moth.展开更多
Parasitoids are key regulators in ecological communities and widely used as agents in biocontrol programmes.The fall armyworm,Spodoptera frugiperda,recently invaded multiple continents and caused substantial economic ...Parasitoids are key regulators in ecological communities and widely used as agents in biocontrol programmes.The fall armyworm,Spodoptera frugiperda,recently invaded multiple continents and caused substantial economic losses in agriculture.Pyemotes zhonghuajia,a newly identified mite parasitoid,has shown potential for controlling various agricultural insect pests.Therefore,this study tested the performance of P.zhonghuajia in parasitising S.frugiperda.We also investigated the sublethal effects of parasitism by P.zhonghuajia on host fitness traits,transgenerational impacts,and cellular and humoral immunity.Our result showed that the fifth-instar larvae of S.frugiperda parasitised by 40 P.zhonghuajia were all dead(i.e.,a lethal effect),while parasitism by 5 or 10 P.zhonghuajia was considered sublethal since many S.frugiperda survived to adulthood and produced offspring after mating.The sublethal influences from parasitism by P.zhonghuajia resulted in reduced pupal weight,adult emergence rate and fecundity,but increased developmental time and longevity.Parasitism at both lethal(40 mites)and sublethal(10 mites)levels impaired the cellular and humoral immunity of S.frugiperda.This study presents the first empirical evidence that mite parasitoids can negatively influence host immunity.Moreover,it provides insights into the biocontrol potential of mite parasitoids and their interactions with hosts.展开更多
基金Supported by HAAFS Science and Technology Innovation Special Project(2022KJCXZX-CGS-8).
文摘[Objectives]This study aimed to quantitatively investigate the dynamic patterns of spore powder of Metarhizium anisopliae and Beauveria bassiana carried on the body surface of Pyemotes zhonghuajia,providing a theoretical basis for optimizing synergistic biological control strategies utilizing natural enemies and pathogens.[Methods]Under laboratory conditions,the spore load on mites after crawling on a spore-powder-coated surface for different durations(0-4 h),and the spore shedding after crawling on a clean surface for different durations(0-4 h)post-loading were measured.[Results]The spore load of mites for both fungi increased with crawling time,but exhibited distinct kinetic patterns.The load of M.anisopliae spores peaked at 2 h(6.86×104 spores/mite)and then decreased significantly;whereas the load of B.bassiana spores continued to increase to a maximum(12.83×104 spores/mite)within 4 h,with the rate of increase slowing significantly after 2 h.During the shedding phase,the number of both types of spores decreased with crawling time.After 4 h,the residual amount of B.bassiana spores(3.18×104 spores/mite)was significantly higher than that of M.anisopliae(2.51×104 spores/mite).[Conclusions]The process of P.zhonghuajia carrying entomopathogenic fungal spores exhibits significant temporal dynamics and species specificity.The findings identify key time points for spore loading and shedding,providing crucial parameters for determining the optimal pre-release"loading"duration and assessing the mites sustained dispersal capacity in the field,which holds significant importance for advancing the application of synergistic biological control technology.
基金Supported by Wuhan Landscape Gardening Bureau Project(Wu Park[2006]No.49)~~
文摘[ Objective] The paper was to study the parasitic ability and field control effect of Pyemotes sp. against oriental fruit moth （Grapholitha molesta Bussck） which caused damage on Photinia. [ Method] The indoor infection test and field control test of Pyemotes sp. against oriental fruit moth was carried out. The parasitic ability and activity characteristic of Pyemotes sp. were observed, and the effect of environmental factors such as humidity and temperature on the para- sitic ability of Pyemotes sp. was studied. [Result] Pyemotes sp. had good control effect on oriental fruit moth under the proper conditions; the host searching abili- ties of Pyemotes sp. were different at different temperature and humidity conditions, humidity had large impact on the activity of Pyemotes sp., while temperature had large impact on the individual activity. [ Conclusion] The result showed that Pyemotes sp. had effective control effort on oriental fruit moth.
基金funded by the National Natural Science Foundation of China(32060637 and 32260708)the Highlevel Talent Innovation and Entrepreneurship Funding Project in Guizhou Province,China((2021)01)+3 种基金the Guizhou Provincial Science and Technology Innovation Talent Team Project,China(Qian Ke He Pingtai RencaiCXTD(2021)004)the Systematic and Applied Acarology Society International Joint Project,England(2022(01))the Growth Project of Youth Talent in Ordinary Universities in Guizhou Province,China((2021)079)the Natural Science Special Project in Guizhou University,China((2020)02)。
文摘Parasitoids are key regulators in ecological communities and widely used as agents in biocontrol programmes.The fall armyworm,Spodoptera frugiperda,recently invaded multiple continents and caused substantial economic losses in agriculture.Pyemotes zhonghuajia,a newly identified mite parasitoid,has shown potential for controlling various agricultural insect pests.Therefore,this study tested the performance of P.zhonghuajia in parasitising S.frugiperda.We also investigated the sublethal effects of parasitism by P.zhonghuajia on host fitness traits,transgenerational impacts,and cellular and humoral immunity.Our result showed that the fifth-instar larvae of S.frugiperda parasitised by 40 P.zhonghuajia were all dead(i.e.,a lethal effect),while parasitism by 5 or 10 P.zhonghuajia was considered sublethal since many S.frugiperda survived to adulthood and produced offspring after mating.The sublethal influences from parasitism by P.zhonghuajia resulted in reduced pupal weight,adult emergence rate and fecundity,but increased developmental time and longevity.Parasitism at both lethal(40 mites)and sublethal(10 mites)levels impaired the cellular and humoral immunity of S.frugiperda.This study presents the first empirical evidence that mite parasitoids can negatively influence host immunity.Moreover,it provides insights into the biocontrol potential of mite parasitoids and their interactions with hosts.
