Objective:Hyperuricemia(HUA)is a metabolic disease that threatens human health.The role of Penthorum chinense Pursh.(PCP)in the treatment of HUA has begun to receive attention in recent years.This study aimed to inves...Objective:Hyperuricemia(HUA)is a metabolic disease that threatens human health.The role of Penthorum chinense Pursh.(PCP)in the treatment of HUA has begun to receive attention in recent years.This study aimed to investigate the effects and potential mechanisms of PCP in HUA treatment.Methods:A HUA murine model was induced in C57/BL6 mice using potassium oxonate(PO)and adenine(AD).Serum uric acid(SUA)was measured using ultra-performance liquid chromatography(UPLC).Serum creatinine(Scr)was detected using a creatine oxidase assay kit,and serum blood urea nitrogen(BUN)was detected using a urease indophenol blue assay kit.Protein expression levels were detected using western blotting,and gut microbiota were detected using 16S rRNA.Results:PCP substantially improved the serum contents of SUA,Scr,and BUN and alleviated kidney injury.PCP promotes renal uric acid excretion by downregulating GLUT9 and URAT1 expression and upregulating ABCG2 and OAT1 expression PCP also regulated the NOD-like receptor family,pyrin domain-containing protein 3(NLRP3)pathway and reduced the expression of inflammatory factors,thus attenuating kidney injury in HUA mice.PCP regulated the structure of the gut microbiota,including the relative abundance of beneficial bacteria,such as Lactobacillus and Alistipes,which promoted uric acid metabolism and antiinflammatory effects.Conclusions:PCP can reduce uric acid levels by promoting renal uric acid excretion and regulating the gut microbiota.PCP improves kidney injury by inhibiting the activation of the NLRP3 signaling pathway and reducing the levels of inflammatory factors.展开更多
[Objectives]To study the acute toxicity of total flavonoids in Penthorum chinense Pursh. and the therapeutic effect on AFL( Alcoholic Fatty Liver). [Methods] The liquid of total flavonoids in P. chinense Pursh. was in...[Objectives]To study the acute toxicity of total flavonoids in Penthorum chinense Pursh. and the therapeutic effect on AFL( Alcoholic Fatty Liver). [Methods] The liquid of total flavonoids in P. chinense Pursh. was intragastrically administered to the test group rats in the maximum concentration and the maximum administration volume,an equal volume of solvent was given to the control group,and it was observed continuously for 14 d; 1. 5% ferrous sulfate feed was used for feeding,the alcohol intragastric administration method was used to copy the AFL rats model,and the therapeutic effect of total flavonoids in P. chinense Pursh. on the fatty liver rats was observed. [Results]No rat died in the medication administration group and the control group,there was no acute toxicity reaction,and the maximum tolerance dose of total flavonoids in P. chinense Pursh. for the rats by intragastric administration was 33. 6 g/kg; rats suffered AFL 6 weeks after the alcohol intragastric administration. For the 800 mg/kg P. chinense Pursh. total flavonoids and 2 000 mg/kg P. chinense Pursh. extract with the same dose as that of the P. chinense Pursh. crude drug,P. chinense Pursh. total flavonoids played a more significant role than P. chinense Pursh. extract in lowering oil red O staining area in AFL rats' liver tissue and reducing the ALT,AST,TC,TG content in AFL rats' serum. [Conclusions]The P. chinense Pursh. total flavonoids had low acute toxicity,and had a greater therapeutic effect on the AFL rats than the P. chinense Pursh.extract.展开更多
Background:Drug-induced liver damage is a severe medical issue that affects people all over the world.Sorafenib has some side effects that cause liver injury.A dietary medicinal plant called Penthorum chinense Pursh.(...Background:Drug-induced liver damage is a severe medical issue that affects people all over the world.Sorafenib has some side effects that cause liver injury.A dietary medicinal plant called Penthorum chinense Pursh.(PCP)has hepatoprotective properties.There are currently few reports on PCP’s protective impact and mechanism against sorafenib-induced liver injury.Methods:To create a liver injury model,sorafenib was administered to BRL-3A cells.Cell viability assays,immunofluorescence tests,Western blotting,real-time quantitative PCR,and high-content imaging systems were utilized to examine PCP’s effect and mechanism.Results:In this study,PCP treatment mitigated the liver damage caused by sorafenib by enhancing cell survival,lowering lipid reactive oxygen species and malondialdehyde levels,and elevating glutathione levels.In addition,PCP can enhance the protein expression of cystine/glutamate transporter xCT and glutathione peroxidase 4,reduce iron content and alleviate mitochondrial toxicity.Further mechanism studies revealed that PCP inhibited ferroptosis by promoting the production of nuclear factor E2-related factor 2 nuclear translocation and subsequently affecting target genes(HO-1 and NQO1).Conclusion:Together,PCP regulates the nuclear factor E2-related factor 2 pathway,which helps to lessen ferroptosis brought on by sorafenib.展开更多
[Objectives]This study was conducted to optimize the extraction process of total flavonoids from Penthorum chinense Pursh and compare their contents from different parts.[Methods]Single factor and orthogonal experimen...[Objectives]This study was conducted to optimize the extraction process of total flavonoids from Penthorum chinense Pursh and compare their contents from different parts.[Methods]Single factor and orthogonal experiments were designed to optimize the extraction process of total flavonoids from P.chinense Pursh with the volume fraction of ethanol,the ratio of material to liquid,heating reflux extraction time and extraction times as factors,and the content of total flavonoids as the index.A verification test was carried out.The optimized extraction process was adopted to compare the contents of total flavonoids from different parts of P.chinense Pursh.[Results]The best extraction process was extracting the powder of P.chinense Pursh for 2.0 h with 20 times of 55%ethanol by reflux twice.Under this condition,the contents of total flavonoids were 3.63%,8.90%,11.28%,and 4.36%from stems,leaves,flowers and whole grass of P.chinense Pursh,respectively.[Conclusions]The process is reasonable,feasible and stable,and can effectively extract total flavonoids from P.chinense Pursh.The contents of total flavonoids from different parts of P.chinense Pursh were quite different,and the value was higher in the leaves and flowers,so the proportions of leaves and flowers should be paid attention to in the industrial processing of P.chinense Pursh.展开更多
目的通过定量统计方法分析赶黄草相关专利及中英文文献现状,总结研究进展及探索发展瓶颈与趋势。方法通过国家知识产权局官网检索赶黄草相关专利,并进行计量分析;结合中国知网和Web of Science数据库,运用CiteSpace软件对赶黄草相关文...目的通过定量统计方法分析赶黄草相关专利及中英文文献现状,总结研究进展及探索发展瓶颈与趋势。方法通过国家知识产权局官网检索赶黄草相关专利,并进行计量分析;结合中国知网和Web of Science数据库,运用CiteSpace软件对赶黄草相关文献进行可视化分析。结果专利分析共纳入专利360项,申请量总体逐年上升,以发明专利为主,有效专利占比较低;申请人多为企业,地域集中于四川成都、泸州;技术领域聚焦医学(肝病防治)及保健食品领域(保肝、减肥等)。文献分析共纳入284篇中文文献和114篇英文文献,发文量逐年增长;研究地域高度集中于四川;关键词分析显示,医学应用(肝病防治、药理活性探索)及保健食品开发为当前热点。结论赶黄草研究热度持续上升,但存在知识产权保护不足、区域局限及合作分散等问题。建议强化产学研合作,推动专利布局与成果转化;深化肝病防治核心方向,拓展多领域应用;加强品牌推广,突破地域限制,释放其作为道地药材的潜在价值,以推动泸州中医药产业高质量发展。展开更多
基金funded by the National Natural Science Foundation of China(82304870)Innovation Team and Talents Cultivation Program of National Administmiceion of Traditional Chinese Medicine(ZYYCXTD-C-202009)+1 种基金Tianjin Municipal Health Commission Project(2024009)Tianjin University Students’Innovation and Entrepreneurship Training Program Project(202410063027).
文摘Objective:Hyperuricemia(HUA)is a metabolic disease that threatens human health.The role of Penthorum chinense Pursh.(PCP)in the treatment of HUA has begun to receive attention in recent years.This study aimed to investigate the effects and potential mechanisms of PCP in HUA treatment.Methods:A HUA murine model was induced in C57/BL6 mice using potassium oxonate(PO)and adenine(AD).Serum uric acid(SUA)was measured using ultra-performance liquid chromatography(UPLC).Serum creatinine(Scr)was detected using a creatine oxidase assay kit,and serum blood urea nitrogen(BUN)was detected using a urease indophenol blue assay kit.Protein expression levels were detected using western blotting,and gut microbiota were detected using 16S rRNA.Results:PCP substantially improved the serum contents of SUA,Scr,and BUN and alleviated kidney injury.PCP promotes renal uric acid excretion by downregulating GLUT9 and URAT1 expression and upregulating ABCG2 and OAT1 expression PCP also regulated the NOD-like receptor family,pyrin domain-containing protein 3(NLRP3)pathway and reduced the expression of inflammatory factors,thus attenuating kidney injury in HUA mice.PCP regulated the structure of the gut microbiota,including the relative abundance of beneficial bacteria,such as Lactobacillus and Alistipes,which promoted uric acid metabolism and antiinflammatory effects.Conclusions:PCP can reduce uric acid levels by promoting renal uric acid excretion and regulating the gut microbiota.PCP improves kidney injury by inhibiting the activation of the NLRP3 signaling pathway and reducing the levels of inflammatory factors.
基金Supported by Luzhou Municipal Government-Luzhou Medical College Joint Project(2013LZLY-K78)Project of Sichuan Provincial Department of Education in 2015[2015-Chuan Jiao Han(2014)794)]Key Project of Southwest Medical University in 2015(2015-9)
文摘[Objectives]To study the acute toxicity of total flavonoids in Penthorum chinense Pursh. and the therapeutic effect on AFL( Alcoholic Fatty Liver). [Methods] The liquid of total flavonoids in P. chinense Pursh. was intragastrically administered to the test group rats in the maximum concentration and the maximum administration volume,an equal volume of solvent was given to the control group,and it was observed continuously for 14 d; 1. 5% ferrous sulfate feed was used for feeding,the alcohol intragastric administration method was used to copy the AFL rats model,and the therapeutic effect of total flavonoids in P. chinense Pursh. on the fatty liver rats was observed. [Results]No rat died in the medication administration group and the control group,there was no acute toxicity reaction,and the maximum tolerance dose of total flavonoids in P. chinense Pursh. for the rats by intragastric administration was 33. 6 g/kg; rats suffered AFL 6 weeks after the alcohol intragastric administration. For the 800 mg/kg P. chinense Pursh. total flavonoids and 2 000 mg/kg P. chinense Pursh. extract with the same dose as that of the P. chinense Pursh. crude drug,P. chinense Pursh. total flavonoids played a more significant role than P. chinense Pursh. extract in lowering oil red O staining area in AFL rats' liver tissue and reducing the ALT,AST,TC,TG content in AFL rats' serum. [Conclusions]The P. chinense Pursh. total flavonoids had low acute toxicity,and had a greater therapeutic effect on the AFL rats than the P. chinense Pursh.extract.
基金supported by the open fund of State Key Laboratory of Southwestern Chinese Medicine Resources(No.SCMR202103)to Jian LiTibet Autonomous Region Science and Technology Plan(high-tech social development)project(No.XZ202201ZY0031G)to Yi-Xi YangAnti-infective Agent Creation Engineering Research Centre of Sichuan Province,Sichuan Industrial Institute of Antibiotics,School of pharmacy,Chengdu University(No.AAC2023002)to Qiu-Xia Lu.
文摘Background:Drug-induced liver damage is a severe medical issue that affects people all over the world.Sorafenib has some side effects that cause liver injury.A dietary medicinal plant called Penthorum chinense Pursh.(PCP)has hepatoprotective properties.There are currently few reports on PCP’s protective impact and mechanism against sorafenib-induced liver injury.Methods:To create a liver injury model,sorafenib was administered to BRL-3A cells.Cell viability assays,immunofluorescence tests,Western blotting,real-time quantitative PCR,and high-content imaging systems were utilized to examine PCP’s effect and mechanism.Results:In this study,PCP treatment mitigated the liver damage caused by sorafenib by enhancing cell survival,lowering lipid reactive oxygen species and malondialdehyde levels,and elevating glutathione levels.In addition,PCP can enhance the protein expression of cystine/glutamate transporter xCT and glutathione peroxidase 4,reduce iron content and alleviate mitochondrial toxicity.Further mechanism studies revealed that PCP inhibited ferroptosis by promoting the production of nuclear factor E2-related factor 2 nuclear translocation and subsequently affecting target genes(HO-1 and NQO1).Conclusion:Together,PCP regulates the nuclear factor E2-related factor 2 pathway,which helps to lessen ferroptosis brought on by sorafenib.
基金Supported by Key Research and Development Program of Sichuan Province(2022YFS0436)Natural Science Foundation of Sichuan Province(2022NSFSC1738)+4 种基金Science and Technology Planning Project of Luzhou City(2021-JYJ-109,2023SYF120)Special Project of Traditional Chinese Medicine Scientific Research of Sichuan Provincial Administration of Traditional Chinese Medicine(2020CP0029)Southwest Medical University-Luzhou Hospital of Traditional Chinese Medicine Base Project(2019-LH003)Open Subject of Luzhou Key Laboratory of Fine Chemical Application Technology(HYJY-2106-B)Southwest Medical University Undergraduate Student Innovation and Entrepreneurship Training Program(202310632074).
文摘[Objectives]This study was conducted to optimize the extraction process of total flavonoids from Penthorum chinense Pursh and compare their contents from different parts.[Methods]Single factor and orthogonal experiments were designed to optimize the extraction process of total flavonoids from P.chinense Pursh with the volume fraction of ethanol,the ratio of material to liquid,heating reflux extraction time and extraction times as factors,and the content of total flavonoids as the index.A verification test was carried out.The optimized extraction process was adopted to compare the contents of total flavonoids from different parts of P.chinense Pursh.[Results]The best extraction process was extracting the powder of P.chinense Pursh for 2.0 h with 20 times of 55%ethanol by reflux twice.Under this condition,the contents of total flavonoids were 3.63%,8.90%,11.28%,and 4.36%from stems,leaves,flowers and whole grass of P.chinense Pursh,respectively.[Conclusions]The process is reasonable,feasible and stable,and can effectively extract total flavonoids from P.chinense Pursh.The contents of total flavonoids from different parts of P.chinense Pursh were quite different,and the value was higher in the leaves and flowers,so the proportions of leaves and flowers should be paid attention to in the industrial processing of P.chinense Pursh.
文摘目的通过定量统计方法分析赶黄草相关专利及中英文文献现状,总结研究进展及探索发展瓶颈与趋势。方法通过国家知识产权局官网检索赶黄草相关专利,并进行计量分析;结合中国知网和Web of Science数据库,运用CiteSpace软件对赶黄草相关文献进行可视化分析。结果专利分析共纳入专利360项,申请量总体逐年上升,以发明专利为主,有效专利占比较低;申请人多为企业,地域集中于四川成都、泸州;技术领域聚焦医学(肝病防治)及保健食品领域(保肝、减肥等)。文献分析共纳入284篇中文文献和114篇英文文献,发文量逐年增长;研究地域高度集中于四川;关键词分析显示,医学应用(肝病防治、药理活性探索)及保健食品开发为当前热点。结论赶黄草研究热度持续上升,但存在知识产权保护不足、区域局限及合作分散等问题。建议强化产学研合作,推动专利布局与成果转化;深化肝病防治核心方向,拓展多领域应用;加强品牌推广,突破地域限制,释放其作为道地药材的潜在价值,以推动泸州中医药产业高质量发展。
