Objective:To evaluate the mosquito larvicidal,ovicidal,pupicidal and repellent activities of hexane,diethyl ether,dichloromethane,acetone and methanol extracts of Indian medicinal plants,Achras sapota(A.sapota)and Cas...Objective:To evaluate the mosquito larvicidal,ovicidal,pupicidal and repellent activities of hexane,diethyl ether,dichloromethane,acetone and methanol extracts of Indian medicinal plants,Achras sapota(A.sapota)and Cassia auriculata(C.auriculata)at different concentrations against Anopheles stephensi(An.stephensi),a malarial vector.Methods:Twenty five early third instar larvae of An.stephensi were exposed to various concentrations(30-210 mg/L)of plants extracts and were assayed in the laboratory by using the protocol of WHO 2005;then after 24 h LC_(50) values of the A.sapota and C.auriculata leaf extract was determined by probit analysis.The ovicidal activity was tested with the extracts ranging from 50-350 mg/L.The pupicidal activity was recorded after 24 h of exposure to the extract.The repellent efficacy was determined against mosquito species at two different concentrations 1.5 and 3.0 mg/cm^(2) under laboratory conditions.Results:Among the five different extracts tested against the An.stephensi,methanol extract of A.sapota proved to be an more effective solvent extract in almost all the parameters studied than C.auriculata.Conclusions:It is inferred that the leaf extract of A.sapota and C.auriculata could be used in vector control programme.展开更多
Objective:To evaluate the mosquitocidal activities of various solvent extract of Oxystelma esculentum(O.esculentum)against the medically important dengue vector mosquito,Aedes aegypti(Ae.aegypti)L.Methods:A total of 2...Objective:To evaluate the mosquitocidal activities of various solvent extract of Oxystelma esculentum(O.esculentum)against the medically important dengue vector mosquito,Aedes aegypti(Ae.aegypti)L.Methods:A total of 25 early third instar larvae of Ae.aegypti were exposed to various concentrations(60-300 mg/L)and were assayed in the laboratory by using the protocol of World Health Organization,2005;the 24 h LC_(50) values of the O.esculentum leaf extract was determined by probit analysis.The ovicidal activity was determined against the freshly laid eggs of Ae.aegypti to various concentrations ranging from 50-300 mg/L under laboratory conditions.The pupicidal activity was determined against pupae of Ae.aegypti to various concentrations ranging from 70-280 mg/L after 24 h of exposure to the concern extract.The repellent efficacy was determined against adult female mosquito species at 1.0,2.0 and 3.0 mg/cm^(2) under laboratory conditions.Results:The LC_(50) value of methanol extract of O.esculentum against 3rd instar larvae of Ae.aegypti was 125.82 mg/L.The same extract showed 100%egg mortality at 250 mg/L and also pupicidal activity observed against the pupae of Ae.aegypti at 280 mg/L.Conclusions:The present results suggest that the O.esculentum leaf extracts provided an excellent,potential phytopesticide for controlling Ae.aegypti mosquito.展开更多
基金Supported by University Grants Commission,New Delhi[Ref.No.42-583/2013(SR)]Department of Science&Technology-Fast Track Young Scientist Project,DST,New Delhi(Ref.NO.SB/FT/LS-356/2012).
文摘Objective:To evaluate the mosquito larvicidal,ovicidal,pupicidal and repellent activities of hexane,diethyl ether,dichloromethane,acetone and methanol extracts of Indian medicinal plants,Achras sapota(A.sapota)and Cassia auriculata(C.auriculata)at different concentrations against Anopheles stephensi(An.stephensi),a malarial vector.Methods:Twenty five early third instar larvae of An.stephensi were exposed to various concentrations(30-210 mg/L)of plants extracts and were assayed in the laboratory by using the protocol of WHO 2005;then after 24 h LC_(50) values of the A.sapota and C.auriculata leaf extract was determined by probit analysis.The ovicidal activity was tested with the extracts ranging from 50-350 mg/L.The pupicidal activity was recorded after 24 h of exposure to the extract.The repellent efficacy was determined against mosquito species at two different concentrations 1.5 and 3.0 mg/cm^(2) under laboratory conditions.Results:Among the five different extracts tested against the An.stephensi,methanol extract of A.sapota proved to be an more effective solvent extract in almost all the parameters studied than C.auriculata.Conclusions:It is inferred that the leaf extract of A.sapota and C.auriculata could be used in vector control programme.
基金Supported by University Grants Commission(UGC,New Delhi)[(Ref.No.42-583/2013(SR))]Department of Science&Technology-Fast Track Young Scientist Project(DST,New Delhi)(Ref.NO.SB/FT/LS-356/2012).
文摘Objective:To evaluate the mosquitocidal activities of various solvent extract of Oxystelma esculentum(O.esculentum)against the medically important dengue vector mosquito,Aedes aegypti(Ae.aegypti)L.Methods:A total of 25 early third instar larvae of Ae.aegypti were exposed to various concentrations(60-300 mg/L)and were assayed in the laboratory by using the protocol of World Health Organization,2005;the 24 h LC_(50) values of the O.esculentum leaf extract was determined by probit analysis.The ovicidal activity was determined against the freshly laid eggs of Ae.aegypti to various concentrations ranging from 50-300 mg/L under laboratory conditions.The pupicidal activity was determined against pupae of Ae.aegypti to various concentrations ranging from 70-280 mg/L after 24 h of exposure to the concern extract.The repellent efficacy was determined against adult female mosquito species at 1.0,2.0 and 3.0 mg/cm^(2) under laboratory conditions.Results:The LC_(50) value of methanol extract of O.esculentum against 3rd instar larvae of Ae.aegypti was 125.82 mg/L.The same extract showed 100%egg mortality at 250 mg/L and also pupicidal activity observed against the pupae of Ae.aegypti at 280 mg/L.Conclusions:The present results suggest that the O.esculentum leaf extracts provided an excellent,potential phytopesticide for controlling Ae.aegypti mosquito.
