To optimize culture conditions for xylanase production by solid state fermentation （SSF） using Bacillus pumilus, with paddy husk as support, solid medium contained 200 g of paddy husk with 800 mL of liquid fermentat...To optimize culture conditions for xylanase production by solid state fermentation （SSF） using Bacillus pumilus, with paddy husk as support, solid medium contained 200 g of paddy husk with 800 mL of liquid fermentation medium [xylan, 20.0 g/L; peptone, 2.0 g/L; yeast extract, 2.5 g/L; K2HPO4, 2.5 g/L; KH2PO4, 1.0 g/L; NaCl, 0.1 g/L; （NH4）2SO4, 2.0 g/L, CaCl2-2H2O, 0.005 g/L; MgCl2.6H2O, 0.005 g/L; and FeCI3, 0.005 g/L] at pH 9.0 was applied. The highest xylanase activity （142.0 ±0.47 U/g DM] was obtained on the 6th day at 30℃ The optimized paddy husk to liquid fermentation medium ratio was 2：9, and the optimized culture temperature was 40℃. When commercial Birchwood xylan was replaced with different concentrations of corncob, xylanase production was maximized （224.2 U/g DM） in the medium with 150 g/L corncob. Xylanase production was increased by sucrose, fructose and arabinose, whereas reduced by glucose, galactose, lactose and amylose. When organic nitrogen sources were replaced with locally available nitrogen sources such as groundnut powder or sesame seedcake powder or coconut seedcake powder or soy meal powder, the highest xylanase production （290.7 U/g DM） was obtained in the medium with soy meal powder and 16.0 g/L of soy meal powder was the optimum （326.5±0.34 U/g DM）. Based on the optimization studies, B. pumilus produced 2.3 times higher xylanase activity. The medium cost was reduced from 2 458.3 to 178.3 SLR/kg and the total activity which could be obtained from 1 kg of the medium was increased from 48 624 to 220 253 Units.展开更多
Proteases due to their wide range of applications in biotechnological processes have been the??focus of intense research for many decades. However, from industrial?application view point most of the available protease...Proteases due to their wide range of applications in biotechnological processes have been the??focus of intense research for many decades. However, from industrial?application view point most of the available proteases lack desired properties;?therefore, search for better and efficient thermostable alkaline proteases are?always on.?Bacillus pumilus?D-6, isolated from dairy plant soil sample, in the?current study produced protease which showed activity and stability at high?alkaline?pH (8 - 12) and high?temperatures (70。C- 100。C). Enzyme activity remained unfazed even in presence?of inhibitors like Pb2+and Hg2+which are considered?universal inhibitors of enzyme activity. Besides, the organism successfully?utilized crude agriculture based substrates as carbon and nitrogen source and?produced substantial enzyme titre.展开更多
Background: Viruses can cause different diseases in plants. To prevent viral infections, plants are treated with chemical compounds and antiviral agents. Chemical antiviral agents usually have narrow specificity, whic...Background: Viruses can cause different diseases in plants. To prevent viral infections, plants are treated with chemical compounds and antiviral agents. Chemical antiviral agents usually have narrow specificity, which limits their wide application. Alternative antiviral strategy is associated with the use of microbial enzymes, which are less toxic and are readily decomposed without accumulation of harmful substances. The aim of this work is to study the effect of Bacillus pumilus ribonuclease on various phytopathogenic viruses with specific focus on the ability of enzyme to eliminate them from plant explants in vitro. Materials and methods: Extracellular ribonuclease of B. pumilus is tested as an antiviral agent. To study the antiviral effect of RNase, depending on concentration and the time of application several plant-virus model systems are used. Virus detection is conducted by serological testing and RT-PCR. Results: Bacillus pumilus ribonuclease possesses antiviral activity against plant Rna-viruses RCMV (red clover mottle virus), PVX (Potato Virus X) and AMV (Alfalfa Mosaic Virus). The maximum inhibitory effect against actively replicating viruses is observed when plants are treated with the enzyme in the concentration of 100 ug/ml prior to infection. In case of local necrosis ribonuclease in the concentration of 1 ug/ml completely inhibits the development of RCMV virus on bean plants. The enzyme is able to penetrate plants and inhibit the development of viral infection, inhibiting effect for untreated surfaces decreased on average for 20%. It is also found that B. pumilus ribonuclease protects apical explants of sprouts of potato tubers from PVM and PVS viruses. Conclusion: B. pumilus ribonuclease possesses antiviral activity against plant Rna-viruses and produces viruses-free plants in the apical meristem culture.展开更多
The misuse of Bacillus anthracis as a bioweapon continues to be a serious concern. Medical personnel and researchers are served well if appropriate non-pathogenic anthrax simulants can be used as countermeasures in pr...The misuse of Bacillus anthracis as a bioweapon continues to be a serious concern. Medical personnel and researchers are served well if appropriate non-pathogenic anthrax simulants can be used as countermeasures in preparative planning. While there are several accepted simulants of B. anthracis, the addition of another model organism would be beneficial. This investigation was undertaken to evaluate the suitability of B. pumilus as a simulant for B. anthracis. All organisms were grown on AK Agar #2 to foster sporulation. Optimum conditions for spore formation were determined for B. pumilus as well as for currently used anthrax surrogates B. atrophaeus and B. thuringiensis. Spore dimensions were determined by scanning electron microscopy. Comparative antibody binding studies using commercially available anti-Bacillus antisera were completed with the simulants as well as with a negative control organism, Clostridium sporogenes. We report that B. pumilus sporulated readily (2.9 × 1010 viable spores per plate), had appropriate spore size (1.24 μm × 0.59 μm) and reactivity with anti-Bacillus antibodies. The characteristics of B. pumilus determined in this study suggest this organism represents a novel, suitable model for B. anthracis.展开更多
This study examined that the efficiency of Bacillus pumilus(BP)on growth performance and meat quality of broiler chickens.A total of day old 240 male broiler chicks were purchased from local hatchery Hyderabad.The chi...This study examined that the efficiency of Bacillus pumilus(BP)on growth performance and meat quality of broiler chickens.A total of day old 240 male broiler chicks were purchased from local hatchery Hyderabad.The chicks were allocated into four groups with six replicates of 10 birds in each replicate.Four diets were prepared for experiment,which included control as basal diet,BP-0.1(CONT+0.1%BP),BP-0.2(CONT+0.2%BP)and AGP(CONT+zinc bacitracin 20 ppm)as antibiotic growth promotor.The results showed that live body weight(LBW),average daily gain was recorded higher(P<0.01)in birds fed diet with BP-0.1 than control and BP-0.2 groups,as well as ADFI also increased in BP-0.1 than control group.BP-0.1 supplementation significantly reduced feed conversion ratio(FCR)than other groups(P<0.01).Both moisture and crude protein(CP)content influenced by supplementation of BP-0.1 than other groups.However,birds fed diet with BP-0.1 had least fat content in breast meat compared with the other groups(P<0.01).In addition,BP-0.1probiotic-fed birds had the lowest(P<0.01)pH,cooking loss(CL)and drip loss(DL)as compared to control,BP-0.2 and AGP groups.The water holding capacity(WHC)were found higher in BP-0.1 as compared to other groups(P<0.01).In conclusion,Bacillus pumilus probiotic supplementation is a promising approach for substitution of antibiotics in broiler diet for enhancement of growth performance and physiochemical properties of meat.展开更多
Diarrhea is the leading cause of mortality in postnatal goat kids,seriously impacting breeding efficiency.This study aimed to explore the effects of Bacillus pumilus 315 (B.pumilus) on goat kids’diarrhea and its regu...Diarrhea is the leading cause of mortality in postnatal goat kids,seriously impacting breeding efficiency.This study aimed to explore the effects of Bacillus pumilus 315 (B.pumilus) on goat kids’diarrhea and its regulatory mechanism.Thirty-six 1-day-old goat kids were assigned into four treatments,the control(CON) group and low-,medium-and high-dose groups supplemented with B.pumilus at 1×10^(8)(BP1),5×10^(8)(BP5),and 1×10^(9)CFU/d (BP10).Each group consisted of 9 replicates with 1 goat kid per replicate.The results showed that the incidence of diarrhea and fecal scores decreased significantly(P<0.05).A dose of 5×10^(8)CFU/d B.pumilus reduced pro-inflammatory factors (including tumor necrosis factor-a[TNF-a],interleukin-1β[IL-1β],interleukin-6[IL-6],P<0.05),increased the expression levels of anti-inflammatory factors (including transforming growth factor-β[TGF-β],peroxisome proliferate-activated receptor-gamma[PPAR-γ],interleukin-10[IL-10],P<0.05),immune indicators(including immunoglobulin G[IgG],immunoglobulin A[IgA],immunoglobulin M[IgM],secretory immunoglobulin A[sIgA],P<0.05) and antioxidant indicators (including total antoxidative capacity[T-AOC],superoxide dismutase[SOD],glutathione peroxidase[GSH-Px],catalase[CAT],P<0.05) in both jejunum and colon,and ultimately improved the barrier function of the jejunum and colon mucosa.The enhanced gut immunity and barrier function were associated with increased abundance of Enterococcus and Lactobacillus (P<0.05) and decreased abundance of Campylobacter and Escherichia-Shigella(P<0.05).In conclusion,dietary addition of B.pumilus may improve gut health by modulating the composition and function of the flora,ultimately alleviating diarrhea in goat kids.展开更多
This study investigated the effects of dietary supplementation with the probiotic Bacillus pumilus on coho salmon(Oncorhynchus kisutch).A total of 360 juvenile fish,with an average initial weight of 130.75±1.33 g...This study investigated the effects of dietary supplementation with the probiotic Bacillus pumilus on coho salmon(Oncorhynchus kisutch).A total of 360 juvenile fish,with an average initial weight of 130.75±1.33 g,were randomly assigned to four treatment groups,each with three replicates of 30 fish.The fish were then fed for 70 days using formulated diets containing four concentrations of B.pumilus:0(control group),2×10^(6),4×10°,and 8×10^(10)aFU/g.We then evaluated growth,nutrient metabolism,immune-related serum biochemical markers,enzyme activity,gene expression,and muscle composition.Additionally,we assessed intestinal histology and microbiota composition.Our findings revealed that B.pumilus significantly improved growth,protein and fat metabolism,and immune function compared to the control group.It also had a positive effect on the structure and function of the intestinal microbiota.The group receiving 4×10^(8) CFU/g showed the greatest improvements.Overall,B.pumilus appears to be a promising probiotic for enhancing the health and performance of coho salmon in aquaculture.展开更多
This study investigated the antifungal activity and possible mode of action of Bacillus pumilus HN-10 antifungal peptide P-1 against Trichothecium roseum.The results showed that the antifungal peptide P-1 at a concent...This study investigated the antifungal activity and possible mode of action of Bacillus pumilus HN-10 antifungal peptide P-1 against Trichothecium roseum.The results showed that the antifungal peptide P-1 at a concentration of 1.0μg mL^(-1)had strong antifungal activity against T.roseum.P-1 inhibited the tricarboxylic acid cycle(TCA)pathway and the transporter pathway of NADH to coenzyme Q on the electron transport chain.P-1 significantly reduced succinate dehydrogenase(SDH),malate dehydrogenase(MDH),ATPase,mitochondrial complex enzymes I,II and IV enzyme activities on the electron transport chain,and 5'-triphosphate(ATP),5'-diphosphate(ADP),5'-monophosphate(AMP)content,and energy charge(EC);significantly increased 6-phosphofructokinase(PFK)enzyme activity.The release of Ca^(2+)(OD_(680))from the inner mitochondrial membrane and the openness of the mitochondrial permeability transition pore(MPTP)were analysed,and microscopy was performed following staining of mitochondria with JC-1.The results indicated that P-1 significantly increased the release of Ca^(2+) and the openness of MPTP,decreased the mitochondrial membrane potential,and produced green fluorescence;transcriptomics data analysis showed that there were 39 differentially expressed genes(DEGs)related to energy metabolism enzymes.The results verified by qRT-PCR were basically consistent with the transcriptome sequencing results.Thus,P-1 achieved its inhibitory effect mainly by regulating genes related to energy metabolism.展开更多
Promoter elements from random chromosomal DNA of a rice epiphytic Bacillus pumilus were cloned into promoter probe shuttle vector ECE7 and sequenced. The results showed that these elements were all new DNA sequences. ...Promoter elements from random chromosomal DNA of a rice epiphytic Bacillus pumilus were cloned into promoter probe shuttle vector ECE7 and sequenced. The results showed that these elements were all new DNA sequences. Six strong promoter elements were obtained by determination of CAT enzyme activity in both E. coli and B. pumilus. Transcription start sites of the cat mRNA were located by primer extension using total RNA. Comparison of the promoter sequences indicated that three of them contain -10 and -35 regions like B. pumilus σ43 consensus sequence and another one is similar to B. pumilus σ29. The other two have no typical consensus sequences of known sigma factors so far.展开更多
对从连云港东西连岛海泥样品中分离得到的菌株Bacillus pumilus HX2-2的分类地位、生长条件和抑菌活性进行了研究。经过形态特征、生理生化性质及16S r DNA序列分析鉴定,该菌属于短小芽胞杆菌。不同温度、盐度、pH培养条件下测定菌液吸...对从连云港东西连岛海泥样品中分离得到的菌株Bacillus pumilus HX2-2的分类地位、生长条件和抑菌活性进行了研究。经过形态特征、生理生化性质及16S r DNA序列分析鉴定,该菌属于短小芽胞杆菌。不同温度、盐度、pH培养条件下测定菌液吸光度OD600值,表明该菌是一株轻度嗜盐菌,最适温度、盐度、pH分别为30℃、3%、7.0~8.0。在不同病原真菌的平板抑菌活性试验中,该菌对草莓尖胞镰刀菌、马铃薯炭疽病菌和水稻立枯丝核菌表现出显著的抑菌作用。菌株B.pumilus HX2-2是一株短小芽胞杆菌,具有广谱抑菌活性,具有进一步研究的价值。展开更多
基金Sida/SAREC and International Science Programme in the Chemical Sciences (IPICS),Sweden, for the financial support
文摘To optimize culture conditions for xylanase production by solid state fermentation （SSF） using Bacillus pumilus, with paddy husk as support, solid medium contained 200 g of paddy husk with 800 mL of liquid fermentation medium [xylan, 20.0 g/L; peptone, 2.0 g/L; yeast extract, 2.5 g/L; K2HPO4, 2.5 g/L; KH2PO4, 1.0 g/L; NaCl, 0.1 g/L; （NH4）2SO4, 2.0 g/L, CaCl2-2H2O, 0.005 g/L; MgCl2.6H2O, 0.005 g/L; and FeCI3, 0.005 g/L] at pH 9.0 was applied. The highest xylanase activity （142.0 ±0.47 U/g DM] was obtained on the 6th day at 30℃ The optimized paddy husk to liquid fermentation medium ratio was 2：9, and the optimized culture temperature was 40℃. When commercial Birchwood xylan was replaced with different concentrations of corncob, xylanase production was maximized （224.2 U/g DM） in the medium with 150 g/L corncob. Xylanase production was increased by sucrose, fructose and arabinose, whereas reduced by glucose, galactose, lactose and amylose. When organic nitrogen sources were replaced with locally available nitrogen sources such as groundnut powder or sesame seedcake powder or coconut seedcake powder or soy meal powder, the highest xylanase production （290.7 U/g DM） was obtained in the medium with soy meal powder and 16.0 g/L of soy meal powder was the optimum （326.5±0.34 U/g DM）. Based on the optimization studies, B. pumilus produced 2.3 times higher xylanase activity. The medium cost was reduced from 2 458.3 to 178.3 SLR/kg and the total activity which could be obtained from 1 kg of the medium was increased from 48 624 to 220 253 Units.
文摘Proteases due to their wide range of applications in biotechnological processes have been the??focus of intense research for many decades. However, from industrial?application view point most of the available proteases lack desired properties;?therefore, search for better and efficient thermostable alkaline proteases are?always on.?Bacillus pumilus?D-6, isolated from dairy plant soil sample, in the?current study produced protease which showed activity and stability at high?alkaline?pH (8 - 12) and high?temperatures (70。C- 100。C). Enzyme activity remained unfazed even in presence?of inhibitors like Pb2+and Hg2+which are considered?universal inhibitors of enzyme activity. Besides, the organism successfully?utilized crude agriculture based substrates as carbon and nitrogen source and?produced substantial enzyme titre.
文摘Background: Viruses can cause different diseases in plants. To prevent viral infections, plants are treated with chemical compounds and antiviral agents. Chemical antiviral agents usually have narrow specificity, which limits their wide application. Alternative antiviral strategy is associated with the use of microbial enzymes, which are less toxic and are readily decomposed without accumulation of harmful substances. The aim of this work is to study the effect of Bacillus pumilus ribonuclease on various phytopathogenic viruses with specific focus on the ability of enzyme to eliminate them from plant explants in vitro. Materials and methods: Extracellular ribonuclease of B. pumilus is tested as an antiviral agent. To study the antiviral effect of RNase, depending on concentration and the time of application several plant-virus model systems are used. Virus detection is conducted by serological testing and RT-PCR. Results: Bacillus pumilus ribonuclease possesses antiviral activity against plant Rna-viruses RCMV (red clover mottle virus), PVX (Potato Virus X) and AMV (Alfalfa Mosaic Virus). The maximum inhibitory effect against actively replicating viruses is observed when plants are treated with the enzyme in the concentration of 100 ug/ml prior to infection. In case of local necrosis ribonuclease in the concentration of 1 ug/ml completely inhibits the development of RCMV virus on bean plants. The enzyme is able to penetrate plants and inhibit the development of viral infection, inhibiting effect for untreated surfaces decreased on average for 20%. It is also found that B. pumilus ribonuclease protects apical explants of sprouts of potato tubers from PVM and PVS viruses. Conclusion: B. pumilus ribonuclease possesses antiviral activity against plant Rna-viruses and produces viruses-free plants in the apical meristem culture.
文摘The misuse of Bacillus anthracis as a bioweapon continues to be a serious concern. Medical personnel and researchers are served well if appropriate non-pathogenic anthrax simulants can be used as countermeasures in preparative planning. While there are several accepted simulants of B. anthracis, the addition of another model organism would be beneficial. This investigation was undertaken to evaluate the suitability of B. pumilus as a simulant for B. anthracis. All organisms were grown on AK Agar #2 to foster sporulation. Optimum conditions for spore formation were determined for B. pumilus as well as for currently used anthrax surrogates B. atrophaeus and B. thuringiensis. Spore dimensions were determined by scanning electron microscopy. Comparative antibody binding studies using commercially available anti-Bacillus antisera were completed with the simulants as well as with a negative control organism, Clostridium sporogenes. We report that B. pumilus sporulated readily (2.9 × 1010 viable spores per plate), had appropriate spore size (1.24 μm × 0.59 μm) and reactivity with anti-Bacillus antibodies. The characteristics of B. pumilus determined in this study suggest this organism represents a novel, suitable model for B. anthracis.
文摘This study examined that the efficiency of Bacillus pumilus(BP)on growth performance and meat quality of broiler chickens.A total of day old 240 male broiler chicks were purchased from local hatchery Hyderabad.The chicks were allocated into four groups with six replicates of 10 birds in each replicate.Four diets were prepared for experiment,which included control as basal diet,BP-0.1(CONT+0.1%BP),BP-0.2(CONT+0.2%BP)and AGP(CONT+zinc bacitracin 20 ppm)as antibiotic growth promotor.The results showed that live body weight(LBW),average daily gain was recorded higher(P<0.01)in birds fed diet with BP-0.1 than control and BP-0.2 groups,as well as ADFI also increased in BP-0.1 than control group.BP-0.1 supplementation significantly reduced feed conversion ratio(FCR)than other groups(P<0.01).Both moisture and crude protein(CP)content influenced by supplementation of BP-0.1 than other groups.However,birds fed diet with BP-0.1 had least fat content in breast meat compared with the other groups(P<0.01).In addition,BP-0.1probiotic-fed birds had the lowest(P<0.01)pH,cooking loss(CL)and drip loss(DL)as compared to control,BP-0.2 and AGP groups.The water holding capacity(WHC)were found higher in BP-0.1 as compared to other groups(P<0.01).In conclusion,Bacillus pumilus probiotic supplementation is a promising approach for substitution of antibiotics in broiler diet for enhancement of growth performance and physiochemical properties of meat.
基金funded by the national key research and development program(2022YFA1304200)Inner Mongolia science and technology key project(2021SZD0014)the agricultural science and technology innovation program(CAAS-ZDRW202305)。
文摘Diarrhea is the leading cause of mortality in postnatal goat kids,seriously impacting breeding efficiency.This study aimed to explore the effects of Bacillus pumilus 315 (B.pumilus) on goat kids’diarrhea and its regulatory mechanism.Thirty-six 1-day-old goat kids were assigned into four treatments,the control(CON) group and low-,medium-and high-dose groups supplemented with B.pumilus at 1×10^(8)(BP1),5×10^(8)(BP5),and 1×10^(9)CFU/d (BP10).Each group consisted of 9 replicates with 1 goat kid per replicate.The results showed that the incidence of diarrhea and fecal scores decreased significantly(P<0.05).A dose of 5×10^(8)CFU/d B.pumilus reduced pro-inflammatory factors (including tumor necrosis factor-a[TNF-a],interleukin-1β[IL-1β],interleukin-6[IL-6],P<0.05),increased the expression levels of anti-inflammatory factors (including transforming growth factor-β[TGF-β],peroxisome proliferate-activated receptor-gamma[PPAR-γ],interleukin-10[IL-10],P<0.05),immune indicators(including immunoglobulin G[IgG],immunoglobulin A[IgA],immunoglobulin M[IgM],secretory immunoglobulin A[sIgA],P<0.05) and antioxidant indicators (including total antoxidative capacity[T-AOC],superoxide dismutase[SOD],glutathione peroxidase[GSH-Px],catalase[CAT],P<0.05) in both jejunum and colon,and ultimately improved the barrier function of the jejunum and colon mucosa.The enhanced gut immunity and barrier function were associated with increased abundance of Enterococcus and Lactobacillus (P<0.05) and decreased abundance of Campylobacter and Escherichia-Shigella(P<0.05).In conclusion,dietary addition of B.pumilus may improve gut health by modulating the composition and function of the flora,ultimately alleviating diarrhea in goat kids.
基金supported by grants from the Scientific Research Foundation for the Introduced Talents of Guangxi Minzu University(2018KJQD14)National Natural Science Foundation of China(32303053)Innovation-driven development special fund project of Guangxi(AA17204044).
文摘This study investigated the effects of dietary supplementation with the probiotic Bacillus pumilus on coho salmon(Oncorhynchus kisutch).A total of 360 juvenile fish,with an average initial weight of 130.75±1.33 g,were randomly assigned to four treatment groups,each with three replicates of 30 fish.The fish were then fed for 70 days using formulated diets containing four concentrations of B.pumilus:0(control group),2×10^(6),4×10°,and 8×10^(10)aFU/g.We then evaluated growth,nutrient metabolism,immune-related serum biochemical markers,enzyme activity,gene expression,and muscle composition.Additionally,we assessed intestinal histology and microbiota composition.Our findings revealed that B.pumilus significantly improved growth,protein and fat metabolism,and immune function compared to the control group.It also had a positive effect on the structure and function of the intestinal microbiota.The group receiving 4×10^(8) CFU/g showed the greatest improvements.Overall,B.pumilus appears to be a promising probiotic for enhancing the health and performance of coho salmon in aquaculture.
基金financially supported by National Key R&D Program of China(2018YFD0400205).
文摘This study investigated the antifungal activity and possible mode of action of Bacillus pumilus HN-10 antifungal peptide P-1 against Trichothecium roseum.The results showed that the antifungal peptide P-1 at a concentration of 1.0μg mL^(-1)had strong antifungal activity against T.roseum.P-1 inhibited the tricarboxylic acid cycle(TCA)pathway and the transporter pathway of NADH to coenzyme Q on the electron transport chain.P-1 significantly reduced succinate dehydrogenase(SDH),malate dehydrogenase(MDH),ATPase,mitochondrial complex enzymes I,II and IV enzyme activities on the electron transport chain,and 5'-triphosphate(ATP),5'-diphosphate(ADP),5'-monophosphate(AMP)content,and energy charge(EC);significantly increased 6-phosphofructokinase(PFK)enzyme activity.The release of Ca^(2+)(OD_(680))from the inner mitochondrial membrane and the openness of the mitochondrial permeability transition pore(MPTP)were analysed,and microscopy was performed following staining of mitochondria with JC-1.The results indicated that P-1 significantly increased the release of Ca^(2+) and the openness of MPTP,decreased the mitochondrial membrane potential,and produced green fluorescence;transcriptomics data analysis showed that there were 39 differentially expressed genes(DEGs)related to energy metabolism enzymes.The results verified by qRT-PCR were basically consistent with the transcriptome sequencing results.Thus,P-1 achieved its inhibitory effect mainly by regulating genes related to energy metabolism.
基金This work was supported by McKnight Foundation and the Key Basic Research Fund of the Ministry of Education.
文摘Promoter elements from random chromosomal DNA of a rice epiphytic Bacillus pumilus were cloned into promoter probe shuttle vector ECE7 and sequenced. The results showed that these elements were all new DNA sequences. Six strong promoter elements were obtained by determination of CAT enzyme activity in both E. coli and B. pumilus. Transcription start sites of the cat mRNA were located by primer extension using total RNA. Comparison of the promoter sequences indicated that three of them contain -10 and -35 regions like B. pumilus σ43 consensus sequence and another one is similar to B. pumilus σ29. The other two have no typical consensus sequences of known sigma factors so far.
