[Objective] This study aimed to screen suitable excellent cherry varieties for cultivation in Northern Guizhou region. [Method] In 2011-2014, various biological traits of two new introduced cherry varieties and a loca...[Objective] This study aimed to screen suitable excellent cherry varieties for cultivation in Northern Guizhou region. [Method] In 2011-2014, various biological traits of two new introduced cherry varieties and a local variety were evaluated and compared, including plant growth, characteristics of branches, leaves and flowers, fruit economic traits, phenological response, yield traits and stress resistance. [Result] Black Pearl and Pitch-Black-skinned Cherry exhibited better performance than the local variety, with the branch germination rate of 85.42% and 80.95%, branching rate of 18.75% and 15.87%, spring shoot length of 35.5 cm and 31.6 cm, annual spring shoot growth of 6 223.2 and 5 956.6 cm, leaf size of 14.9 cm × 7.6 cm and 13.8 cm × 8.1 cm, flower size of 2.5 cm ×2.6 cm and 2.3 cm × 2.4 cm, fruit ma- turity stage in late April-early May and early-mid May, average single fruit weight of 3.85 g and 3.68 g, sugar content in fruit juice of 14.5% and 14.2%, fruit edible rate of 76.47% and 77.23%, fruit-bearing rate of 28.48% and 29.81%, average yield per plant of 15.6 kg and 11.9 kg, converted output of 12 282 kg/hm2 and 9 814.5 kg/hm2, respectively. In addition, these two new introduced cherry varieties demonstrated strong adaptability and relatively strong adaptability, respectively. Therefore, Black Pearl exhibited the best performance, followed by Pitch-Black-skinned Cherry, which were both superior to the local variety. [Conclusion] Black Pearl can be selected as the main cultivar with supporting cultivation techniques in Northern Guizhou region, appropriately combined with Pitch-Black-skinned Cherry.展开更多
Recently,Prevotella spp.,a major genus of gram-negative commensal bacteria in humans,have emerged as a key microbial contributor to host metabolism due to its ability to ferment dietary fibers,produce beneficial short...Recently,Prevotella spp.,a major genus of gram-negative commensal bacteria in humans,have emerged as a key microbial contributor to host metabolism due to its ability to ferment dietary fibers,produce beneficial short-chain fatty acids,and influence immune responses.However,their diversity and functional differences have created challenges for their development and therapeutic use.Recent studies have shown that specific Prevotella species,such as P.copri,P.intestinalis,and P.histicola,can strengthen gut barrier integrity and reduce metabolic imbalances.Notably,Prevotella populations can be increased through high-fiber or herbal-based treatments.Traditional herbal medicines,including fiber-rich decoctions,also demonstrate the potential to boost endogenous Prevotella communities,enhance microbial fermentation,and improve glucose and lipid balance.This perspective examines the context-dependent roles of Prevotella spp.,with emphasis on the functional heterogeneity of key species such as P.copri,suggests a framework for combining herbal modulation with species-level microbiota profiling,and outlines a research plan to explore microbe-herb synergy in treating obesity,type 2 diabetes,and related metabolic disorders.This strategy offers a new,ecology-based approach to complement standard metabolic interventions.展开更多
We investigated the influence of different levels of osmotic stress on growth and development in selected wild almond species （eight Prunus spp.） grown in vitro. The study, while endorsing the efficacy of in vitro s...We investigated the influence of different levels of osmotic stress on growth and development in selected wild almond species （eight Prunus spp.） grown in vitro. The study, while endorsing the efficacy of in vitro screening of auxiliary buds of wild almond for osmotic stress tolerance, showed species variability in its response to osmotic stress. Osmotic stress reduced growth and development of all the species. How-ever, the putative tolerant Prunus spp. showed better performance than the putative susceptible genotypes. On average there was an 80% de-crease in shoot dry weight at -1.2 MPa. Reduction in shoot weight was more common in osmotic stress-susceptible species in the section labeled‘Euamygdalus’. The tolerant Prunus species produced smaller changes in biochemical responses than the sensitive cultivars for malondialdehyde content, catalase activity, relative permeability of protoplast membranes, and net photosynthetic rate. The tolerant species maintained cell integrity better than drought sensitive species. Wild almond species in the section labeled ‘Spartioides’ （Prunus arabica （Olivier） Neikle, Prunus glauca＆amp;nbsp;（Browicz） A.E. Murray, Prunus scoparia Spach） and ‘Lycioides’ （Pru-nus lycioides Spach, Prunus reuteri Bossi. et Bushe） were best adapted to osmotic stress. Increase in chlorophyll concentration and leaf thickness under high osmotic stress can be considered as preliminary selection parameters for osmotic stress tolerance in Prunus spp. The study con-firmed the efficacy of the in vitro method for screening of large number of genotypes for osmotic stress tolerance in wild almond species.展开更多
We assessed chemical composition and variation in oil content and seed weight of 40 wild-growing almonds（Prunus L. spp.） accessions collected from different parts of Iran. There were significant differences in kerne...We assessed chemical composition and variation in oil content and seed weight of 40 wild-growing almonds（Prunus L. spp.） accessions collected from different parts of Iran. There were significant differences in kernel weight and oil parameters. Accessions ranged from0.20 to 1.5 g in kernel weight, 0.2–3.0 mm in shell thickness, and 16–55 % in oil content. The predominant vegetable oil components of kernels were 4.6–9.5 % palmitic acid, 0.4–0.8 % palmitoleic acid, 1.0–3.4 % stearic acid,48.8–88.4 % oleic acid and 11.3–33.2 % linoleic acid.Linolenic acid was detected in 15 accessions. High heritability was recorded for all studied traits and was maximum for shell thickness（98.5 %） and minimum for oil content（97.1 %）. Maximum and minimum ‘Euclidean＇pair wise dissimilarities were 17.9 and 0.5, respectively.All 40 accessions were grouped into two major clusters.展开更多
Prunus spinulosa(2n=4x=32)is an evergreen species of significantmedicinal usage and ecological value.However,the lacking of a high-quality genome of P.spinulosa has obstructed further exploration of its ecological stu...Prunus spinulosa(2n=4x=32)is an evergreen species of significantmedicinal usage and ecological value.However,the lacking of a high-quality genome of P.spinulosa has obstructed further exploration of its ecological study and phylogenetic relationship of Prunus.In this study,we present the first haplotype-resolved genome assembly of Prunus s.l.subgenus Laurocerasus,the tetraploid genome of P.spinulosa was phased into 32 pseudochromosomes with 4 haplotypes,the genome size of each haplotype ranged from 249.82 Mb to 259.69 Mb,and N50 fluctuatedfrom 31.35 Mb to 33.25 Mb,the protein-coding genes vary from 21,272 to 22,668.Different evaluation methods showed that the P.spinulosa genome assembly has high quality of completeness,continuity and accuracy.Being the firstcomplete genome of P.spinulosa,it provides a valuable genetic resource for the Prunus tetraploid species database and supports further functional genomic study of this species.展开更多
Cronobacter spp.has strong resistance to desiccation and high permeability in Enterobacteriaceae,and powdered infant formula(PIF)is one of the main contamination routes.In recent years,the contamination of Cronobacter...Cronobacter spp.has strong resistance to desiccation and high permeability in Enterobacteriaceae,and powdered infant formula(PIF)is one of the main contamination routes.In recent years,the contamination of Cronobacter spp.in PIF incidents occurs from time to time,causing infant serious diseases or death.In this investigation,matrix-assisted laser desorption/ionization time of flight mass spectrometry was used to identify the phenotypes of 35 Cronobacter strains isolated from PIF and its processing environment.Subsequently,the isolates were evaluated for drying and osmotic pressure tolerance.The results showed that the deactivation rate of the strains ranged from 9.01%to 77.57%,and the highest osmotic pressure condition the strains could tolerate was 6 g/100 mL Na Cl.In addition,there was a positive correlation between biofilm formation ability and desiccation resistance.Combined with transcriptomics,Cronobacter spp.could activate biofilm synthesis,produce more trehalose,accumulate betaine and electrolytes to stabilize intracellular structure under the two treatment conditions.A total of 31 and 43 genes were found related to desiccation and permeability resistance,respectively.And some genes(cysM,thuF,ycjO,etc.)were found to be associated with two tolerances for the first time.展开更多
Objective:To explore a rapid and accurate method for evaluating the quality of Prunus mandshurica(Maxim.)Koehne(P.mandshurica,Ku Xing Ren)during rancidity using machine vision and learning.Methods:Sensory evaluation a...Objective:To explore a rapid and accurate method for evaluating the quality of Prunus mandshurica(Maxim.)Koehne(P.mandshurica,Ku Xing Ren)during rancidity using machine vision and learning.Methods:Sensory evaluation and chemometrics were used to classify P.mandshurica quality grades after rancidity.Chemical indicators of the P.mandshurica quality change were determined to verify the ob-tained grades and support the subsequent modeling.The International Commission on Illumination color space was used to extract the color features of the P.mandshurica.Discrimination and prediction models based on color features combined with multiple machine learning algorithms were established using 10-fold cross-validation and external test set validation.Results:The P.mandshurica rancidity samples were allocated to three quality grades.The Bayes net model based on powder color successfully identified the P.mandshurica at different grades with an accuracy of 88.89%and 100%using two validations,and the naive Bayes model based on section color achieved the same accuracy with an receiver operating characteristic area of 0.979.The instance-based k-nearest neighbors model based on powder color performed best in predicting the amygdalin content[R^(2)=0.9801,mean absolute error(MAE)=0.2071,root mean squared error(RMSE)=0.4170],followed by the random com-mittee model in predicting the acid value(R^(2)=0.9580,MAE=1.5121,RMSE=1.9099)and the random forest model in predicting the peroxide value(R^(2)=0.8857,MAE=0.0027,RMSE=0.0035).Conclusion:This study demonstrates that color digitization analysis is a potential method for rapidly evaluating the quality of P.mandshurica across the rancidity process,providing a new reference for the quality assessment of traditional Chinese medicines.展开更多
Objective:To evaluate the predictive value of secreted phosphoprotein 1(SPP1)gene expression for postoperative survival in patients with advanced liver cancer undergoing hepatic artery interventional chemoembolization...Objective:To evaluate the predictive value of secreted phosphoprotein 1(SPP1)gene expression for postoperative survival in patients with advanced liver cancer undergoing hepatic artery interventional chemoembolization treatment.Method:Bioinformatics methods,including gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis,were used to identify genes related to survival prognosis in hepatocellular carcinoma(HCC)patients.A retrospective analysis of 115 advanced liver cancer patients treated between January 2016 and October 2017 was conducted.Patients were categorized into SPP1 high-expression(n=89)and low-expression groups(n=26).Additionally,115 healthy individuals served as the control group.The relationship between SPP1 expression and clinical pathological features was analyzed.A 60-month follow-up and logistic regression analysis identified risk factors affecting survival.Results:SPP1 mRNA expression was significantly higher in liver cancer patients compared to healthy controls(P<0.05).SPP1 expression levels were significantly associated with tumor size,Child-Pugh grading,lymph node metastasis,and BCLC staging(P<0.05).High SPP1 expression,along with tumor size,Child-Pugh grading,lymph node metastasis,and BCLC staging,were independent risk factors for survival(P<0.05).The 60-month survival rate was 17.39%,with a median survival of 40 months in the low-expression group versus 18 months in the high-expression group(P<0.05).Conclusion:SPP1 expression is significantly upregulated in advanced liver cancer patients and has predictive value for postoperative survival following hepatic artery chemoembolization treatment.SPP1,combined with clinical indicators such as tumor size,Child-Pugh grading,lymph node metastasis,and BCLC staging,may serve as a prognostic biomarker for interventional treatment outcomes.展开更多
文摘[Objective] This study aimed to screen suitable excellent cherry varieties for cultivation in Northern Guizhou region. [Method] In 2011-2014, various biological traits of two new introduced cherry varieties and a local variety were evaluated and compared, including plant growth, characteristics of branches, leaves and flowers, fruit economic traits, phenological response, yield traits and stress resistance. [Result] Black Pearl and Pitch-Black-skinned Cherry exhibited better performance than the local variety, with the branch germination rate of 85.42% and 80.95%, branching rate of 18.75% and 15.87%, spring shoot length of 35.5 cm and 31.6 cm, annual spring shoot growth of 6 223.2 and 5 956.6 cm, leaf size of 14.9 cm × 7.6 cm and 13.8 cm × 8.1 cm, flower size of 2.5 cm ×2.6 cm and 2.3 cm × 2.4 cm, fruit ma- turity stage in late April-early May and early-mid May, average single fruit weight of 3.85 g and 3.68 g, sugar content in fruit juice of 14.5% and 14.2%, fruit edible rate of 76.47% and 77.23%, fruit-bearing rate of 28.48% and 29.81%, average yield per plant of 15.6 kg and 11.9 kg, converted output of 12 282 kg/hm2 and 9 814.5 kg/hm2, respectively. In addition, these two new introduced cherry varieties demonstrated strong adaptability and relatively strong adaptability, respectively. Therefore, Black Pearl exhibited the best performance, followed by Pitch-Black-skinned Cherry, which were both superior to the local variety. [Conclusion] Black Pearl can be selected as the main cultivar with supporting cultivation techniques in Northern Guizhou region, appropriately combined with Pitch-Black-skinned Cherry.
基金supported by the National Research Foundation of Korea(2020R1F1A1074155).
文摘Recently,Prevotella spp.,a major genus of gram-negative commensal bacteria in humans,have emerged as a key microbial contributor to host metabolism due to its ability to ferment dietary fibers,produce beneficial short-chain fatty acids,and influence immune responses.However,their diversity and functional differences have created challenges for their development and therapeutic use.Recent studies have shown that specific Prevotella species,such as P.copri,P.intestinalis,and P.histicola,can strengthen gut barrier integrity and reduce metabolic imbalances.Notably,Prevotella populations can be increased through high-fiber or herbal-based treatments.Traditional herbal medicines,including fiber-rich decoctions,also demonstrate the potential to boost endogenous Prevotella communities,enhance microbial fermentation,and improve glucose and lipid balance.This perspective examines the context-dependent roles of Prevotella spp.,with emphasis on the functional heterogeneity of key species such as P.copri,suggests a framework for combining herbal modulation with species-level microbiota profiling,and outlines a research plan to explore microbe-herb synergy in treating obesity,type 2 diabetes,and related metabolic disorders.This strategy offers a new,ecology-based approach to complement standard metabolic interventions.
文摘We investigated the influence of different levels of osmotic stress on growth and development in selected wild almond species （eight Prunus spp.） grown in vitro. The study, while endorsing the efficacy of in vitro screening of auxiliary buds of wild almond for osmotic stress tolerance, showed species variability in its response to osmotic stress. Osmotic stress reduced growth and development of all the species. How-ever, the putative tolerant Prunus spp. showed better performance than the putative susceptible genotypes. On average there was an 80% de-crease in shoot dry weight at -1.2 MPa. Reduction in shoot weight was more common in osmotic stress-susceptible species in the section labeled‘Euamygdalus’. The tolerant Prunus species produced smaller changes in biochemical responses than the sensitive cultivars for malondialdehyde content, catalase activity, relative permeability of protoplast membranes, and net photosynthetic rate. The tolerant species maintained cell integrity better than drought sensitive species. Wild almond species in the section labeled ‘Spartioides’ （Prunus arabica （Olivier） Neikle, Prunus glauca＆amp;nbsp;（Browicz） A.E. Murray, Prunus scoparia Spach） and ‘Lycioides’ （Pru-nus lycioides Spach, Prunus reuteri Bossi. et Bushe） were best adapted to osmotic stress. Increase in chlorophyll concentration and leaf thickness under high osmotic stress can be considered as preliminary selection parameters for osmotic stress tolerance in Prunus spp. The study con-firmed the efficacy of the in vitro method for screening of large number of genotypes for osmotic stress tolerance in wild almond species.
基金financially supported by Payam-e-Noor University
文摘We assessed chemical composition and variation in oil content and seed weight of 40 wild-growing almonds（Prunus L. spp.） accessions collected from different parts of Iran. There were significant differences in kernel weight and oil parameters. Accessions ranged from0.20 to 1.5 g in kernel weight, 0.2–3.0 mm in shell thickness, and 16–55 % in oil content. The predominant vegetable oil components of kernels were 4.6–9.5 % palmitic acid, 0.4–0.8 % palmitoleic acid, 1.0–3.4 % stearic acid,48.8–88.4 % oleic acid and 11.3–33.2 % linoleic acid.Linolenic acid was detected in 15 accessions. High heritability was recorded for all studied traits and was maximum for shell thickness（98.5 %） and minimum for oil content（97.1 %）. Maximum and minimum ‘Euclidean＇pair wise dissimilarities were 17.9 and 0.5, respectively.All 40 accessions were grouped into two major clusters.
基金supported by the National Natural Science Foundation of China(Grant No.32570239)Key Technology Research and Development Program of Zhejiang Province(Grant No.2023C03138).
文摘Prunus spinulosa(2n=4x=32)is an evergreen species of significantmedicinal usage and ecological value.However,the lacking of a high-quality genome of P.spinulosa has obstructed further exploration of its ecological study and phylogenetic relationship of Prunus.In this study,we present the first haplotype-resolved genome assembly of Prunus s.l.subgenus Laurocerasus,the tetraploid genome of P.spinulosa was phased into 32 pseudochromosomes with 4 haplotypes,the genome size of each haplotype ranged from 249.82 Mb to 259.69 Mb,and N50 fluctuatedfrom 31.35 Mb to 33.25 Mb,the protein-coding genes vary from 21,272 to 22,668.Different evaluation methods showed that the P.spinulosa genome assembly has high quality of completeness,continuity and accuracy.Being the firstcomplete genome of P.spinulosa,it provides a valuable genetic resource for the Prunus tetraploid species database and supports further functional genomic study of this species.
基金supported by the Joint Funds of the National Natural Science Foundation of China(U21A20272)。
文摘Cronobacter spp.has strong resistance to desiccation and high permeability in Enterobacteriaceae,and powdered infant formula(PIF)is one of the main contamination routes.In recent years,the contamination of Cronobacter spp.in PIF incidents occurs from time to time,causing infant serious diseases or death.In this investigation,matrix-assisted laser desorption/ionization time of flight mass spectrometry was used to identify the phenotypes of 35 Cronobacter strains isolated from PIF and its processing environment.Subsequently,the isolates were evaluated for drying and osmotic pressure tolerance.The results showed that the deactivation rate of the strains ranged from 9.01%to 77.57%,and the highest osmotic pressure condition the strains could tolerate was 6 g/100 mL Na Cl.In addition,there was a positive correlation between biofilm formation ability and desiccation resistance.Combined with transcriptomics,Cronobacter spp.could activate biofilm synthesis,produce more trehalose,accumulate betaine and electrolytes to stabilize intracellular structure under the two treatment conditions.A total of 31 and 43 genes were found related to desiccation and permeability resistance,respectively.And some genes(cysM,thuF,ycjO,etc.)were found to be associated with two tolerances for the first time.
基金funded by the National Natural Science Foundation of China(81573542)Shanxi Province's Traditional Chinese Medicine Technology Innovation Project(2100601).
文摘Objective:To explore a rapid and accurate method for evaluating the quality of Prunus mandshurica(Maxim.)Koehne(P.mandshurica,Ku Xing Ren)during rancidity using machine vision and learning.Methods:Sensory evaluation and chemometrics were used to classify P.mandshurica quality grades after rancidity.Chemical indicators of the P.mandshurica quality change were determined to verify the ob-tained grades and support the subsequent modeling.The International Commission on Illumination color space was used to extract the color features of the P.mandshurica.Discrimination and prediction models based on color features combined with multiple machine learning algorithms were established using 10-fold cross-validation and external test set validation.Results:The P.mandshurica rancidity samples were allocated to three quality grades.The Bayes net model based on powder color successfully identified the P.mandshurica at different grades with an accuracy of 88.89%and 100%using two validations,and the naive Bayes model based on section color achieved the same accuracy with an receiver operating characteristic area of 0.979.The instance-based k-nearest neighbors model based on powder color performed best in predicting the amygdalin content[R^(2)=0.9801,mean absolute error(MAE)=0.2071,root mean squared error(RMSE)=0.4170],followed by the random com-mittee model in predicting the acid value(R^(2)=0.9580,MAE=1.5121,RMSE=1.9099)and the random forest model in predicting the peroxide value(R^(2)=0.8857,MAE=0.0027,RMSE=0.0035).Conclusion:This study demonstrates that color digitization analysis is a potential method for rapidly evaluating the quality of P.mandshurica across the rancidity process,providing a new reference for the quality assessment of traditional Chinese medicines.
基金Medical Research Project of Xi’an Science and Technology Bureau“Molecular Mechanism of miR-1305 Competitive Endogenous circRNA in the Development of Liver Cancer”(Project No.22YXYJ0134)General Project of Key Research and Development Program of Shaanxi Provincial Department of Science and Technology“Mechanism Study on the Inhibition of Liver Cancer Invasion and Metastasis by Downregulating METTL3 and Reducing the m6A Modification Level of MMP3 with Honokiol”(Project No.2023-YBSF-631)。
文摘Objective:To evaluate the predictive value of secreted phosphoprotein 1(SPP1)gene expression for postoperative survival in patients with advanced liver cancer undergoing hepatic artery interventional chemoembolization treatment.Method:Bioinformatics methods,including gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis,were used to identify genes related to survival prognosis in hepatocellular carcinoma(HCC)patients.A retrospective analysis of 115 advanced liver cancer patients treated between January 2016 and October 2017 was conducted.Patients were categorized into SPP1 high-expression(n=89)and low-expression groups(n=26).Additionally,115 healthy individuals served as the control group.The relationship between SPP1 expression and clinical pathological features was analyzed.A 60-month follow-up and logistic regression analysis identified risk factors affecting survival.Results:SPP1 mRNA expression was significantly higher in liver cancer patients compared to healthy controls(P<0.05).SPP1 expression levels were significantly associated with tumor size,Child-Pugh grading,lymph node metastasis,and BCLC staging(P<0.05).High SPP1 expression,along with tumor size,Child-Pugh grading,lymph node metastasis,and BCLC staging,were independent risk factors for survival(P<0.05).The 60-month survival rate was 17.39%,with a median survival of 40 months in the low-expression group versus 18 months in the high-expression group(P<0.05).Conclusion:SPP1 expression is significantly upregulated in advanced liver cancer patients and has predictive value for postoperative survival following hepatic artery chemoembolization treatment.SPP1,combined with clinical indicators such as tumor size,Child-Pugh grading,lymph node metastasis,and BCLC staging,may serve as a prognostic biomarker for interventional treatment outcomes.
