Prunus spinulosa(2n=4x=32)is an evergreen species of significantmedicinal usage and ecological value.However,the lacking of a high-quality genome of P.spinulosa has obstructed further exploration of its ecological stu...Prunus spinulosa(2n=4x=32)is an evergreen species of significantmedicinal usage and ecological value.However,the lacking of a high-quality genome of P.spinulosa has obstructed further exploration of its ecological study and phylogenetic relationship of Prunus.In this study,we present the first haplotype-resolved genome assembly of Prunus s.l.subgenus Laurocerasus,the tetraploid genome of P.spinulosa was phased into 32 pseudochromosomes with 4 haplotypes,the genome size of each haplotype ranged from 249.82 Mb to 259.69 Mb,and N50 fluctuatedfrom 31.35 Mb to 33.25 Mb,the protein-coding genes vary from 21,272 to 22,668.Different evaluation methods showed that the P.spinulosa genome assembly has high quality of completeness,continuity and accuracy.Being the firstcomplete genome of P.spinulosa,it provides a valuable genetic resource for the Prunus tetraploid species database and supports further functional genomic study of this species.展开更多
Objective:To explore a rapid and accurate method for evaluating the quality of Prunus mandshurica(Maxim.)Koehne(P.mandshurica,Ku Xing Ren)during rancidity using machine vision and learning.Methods:Sensory evaluation a...Objective:To explore a rapid and accurate method for evaluating the quality of Prunus mandshurica(Maxim.)Koehne(P.mandshurica,Ku Xing Ren)during rancidity using machine vision and learning.Methods:Sensory evaluation and chemometrics were used to classify P.mandshurica quality grades after rancidity.Chemical indicators of the P.mandshurica quality change were determined to verify the ob-tained grades and support the subsequent modeling.The International Commission on Illumination color space was used to extract the color features of the P.mandshurica.Discrimination and prediction models based on color features combined with multiple machine learning algorithms were established using 10-fold cross-validation and external test set validation.Results:The P.mandshurica rancidity samples were allocated to three quality grades.The Bayes net model based on powder color successfully identified the P.mandshurica at different grades with an accuracy of 88.89%and 100%using two validations,and the naive Bayes model based on section color achieved the same accuracy with an receiver operating characteristic area of 0.979.The instance-based k-nearest neighbors model based on powder color performed best in predicting the amygdalin content[R^(2)=0.9801,mean absolute error(MAE)=0.2071,root mean squared error(RMSE)=0.4170],followed by the random com-mittee model in predicting the acid value(R^(2)=0.9580,MAE=1.5121,RMSE=1.9099)and the random forest model in predicting the peroxide value(R^(2)=0.8857,MAE=0.0027,RMSE=0.0035).Conclusion:This study demonstrates that color digitization analysis is a potential method for rapidly evaluating the quality of P.mandshurica across the rancidity process,providing a new reference for the quality assessment of traditional Chinese medicines.展开更多
A semi-greenhouse study was conducted to understand the effects of soil burial depth on seed germination and seedling development. The seeds of wild apricot （Prunus armeniaca） were buried at the soil depths of 0-cm,...A semi-greenhouse study was conducted to understand the effects of soil burial depth on seed germination and seedling development. The seeds of wild apricot （Prunus armeniaca） were buried at the soil depths of 0-cm, 4-cm, 8-cm, and 12-cm, respectively, to simulate the seed hoarding behavior of rodents in the field. The results revealed that the rates of seed germination and established seedlings from buried seeds were both the highest in 4-cm burial depth group, and then decreased with increasing soil depth. The number of rotten seeds increased in deeper burial depth. It is unfavourable for seed germination at 0-cm burial depth （i.e., seeds were laid on soil surface）. There was insignificant effect of burial depth on growth of established seedlings. The results from this study indicated that proper burial depth in soil would be helpful for the seed germination and seedling growth. The seedlings derived from buried seeds at shallower depth （4 cm） in this research have advantage in their early development.展开更多
Polygalacturonase (PG,EC3.2.1.15) is the key cell wall hydrolase in fruit ripening. The identification and characterization of a full length cDNA (pMT18) encoding for PG from Feicheng peach (Prunus persica (L.) Bat...Polygalacturonase (PG,EC3.2.1.15) is the key cell wall hydrolase in fruit ripening. The identification and characterization of a full length cDNA (pMT18) encoding for PG from Feicheng peach (Prunus persica (L.) Batsch cv. Feicheng) is described. The pMT18 clone is 1188 bp in length, with an open reading frame of 393 amino acids. The homology and phylogenetic analyses indicate a remarkable similarity between peach PG and other ripening related PG. And seven consensus sequences have revealed in peach PG compared to the PG from other plants. However, the profound divergence with other PG and the unique structure features suggest that peach PG probably belongs to a new evolutionary class. In RT PCR analysis, pMT18 related RNA was undetectable in leaves, and was much abundant in ripe fruits. The ripening specific expression pattern of this cDNA will be useful in investigating the roles of PG in fruit ripening and developing a transgenic peach with the improved post harvesting quality in the future.展开更多
One-year-old seedlings of Amur maple (Acer ginnala Maxim), Ussurian pear (Pyrus ussuriensis Maxim) and David peach (Prunus davidiana Carr) were planted in pots in greenhouse and treated with four different soil moistu...One-year-old seedlings of Amur maple (Acer ginnala Maxim), Ussurian pear (Pyrus ussuriensis Maxim) and David peach (Prunus davidiana Carr) were planted in pots in greenhouse and treated with four different soil moisture contents (75.0%, 61.1%, 46.4% and 35.4%). The results showed that net photosynthesis rate (NPR), transpiration rate (TR) and stomatal conductance (Sc) of seedlings of the three species decreased with the decease of soil moisture content, and Amur maple seedlings had the greatest change in those physiological indices, followed by Ussurian pear, David peach. Amur maple and Ussurian pear seedlings also presented a decrease tendency in water use efficiency (WUE) under lower soil moisture content, whereas this was reversed for David peach. Under water stress the biomass allocation to seedling root had a significant increase for all the experimental species. As to root/shoot ratio, Amur maple seedlings had the biggest increase, while David peach had the smallest increase. The leaf plasticity of Amur maple seedlings was greater, the leaf size and total leaf area decreased significantly as the stress was intensified. No significant change of leaf size and total leaf area was found in seedlings of Ussurian pear and David peach. It was concluded that Amur maple was more tolerant to soil moisture stress in comparison with David peach and Ussurian pear.展开更多
Using ISSR technique to analyze the diversity and genetic relationship of germplasm resources in 39 Prunus mume Sibe. et Zucc., the result showed that 10 primers were screened with high resolution from 51 primers, 120...Using ISSR technique to analyze the diversity and genetic relationship of germplasm resources in 39 Prunus mume Sibe. et Zucc., the result showed that 10 primers were screened with high resolution from 51 primers, 120 fragments were amplified, of which 98 were polymorphic loci, accounting for 81.67% of total. Tested materials were divided into 3 classes, as was fundamentally accorded with the traditional classification base on horticulture. There was no obvious difference in geographic relationship among the clustering results.展开更多
[Objective] The aims of this study were to understand the flowering biological characteristics of Prunus domestica ×armeniaca and analyze its differences with other apricot varieties and plum varieties. [Method] ...[Objective] The aims of this study were to understand the flowering biological characteristics of Prunus domestica ×armeniaca and analyze its differences with other apricot varieties and plum varieties. [Method] Taking three varieties of Prunus domestica ×armeniaca as the research object, the phenophase of blooming,morphology characteristics of floral organs and development characteristics of floral organs were observed, pollen amount, pollen viability and stigma receptivity were measured. [Result] The results showed that three varieties of Prunus domestica×armeniaca were in blossom in early April, the florescence were relatively late for 8-12 d comparing with the varieties of apricot, the florescence were relatively similar to the P. domestica L. Morphology characteristics of floral organs of three varieties of Prunus domestica×armeniaca had few differences, were relatively similar to two varieties of plum, and they all had high percent of middle-style and long-style flower.The pollen amount of three varieties of Prunus domestica ×armeniaca had a little differences comparing with two varieties of plum, but were lower than three varieties of apricot. There were some differences in pollen viability, the germination percentage of pollen of ‘Konglongdan＇ was relatively high, was 51.6%; The pollen amount of ‘Fengweihuanghou＇ was relatively high, was 880.8 per anther; ‘Weidi＇ had fewer pollen amount, and the pollen viability was just 2.1%; Three varieties of Prunus domestica ×armeniaca all had strong stigma receptivity on the bloom day, and the stigma receptivity continued exist until the day that 5 days after blooming. [Conclusion] The florescence of three varieties of Prunus domestica ×armeniaca were relatively concentrated, and the rate of pistil abortion was lowest, the variation intensity of stigma receptivity and sustainable time of three varieties of Prunus domestica×armeniaca almost had a little differences comparing with a variety of plum, but slightly more than three varieties of apricot.展开更多
Leaves from three_year_old solar greenhouse nectarine trees ( Prunus persica L. var. nectarina Ait. “Zao Hong Yan”) were used as materials in this study. It was the first time that the ultrastructural charact...Leaves from three_year_old solar greenhouse nectarine trees ( Prunus persica L. var. nectarina Ait. “Zao Hong Yan”) were used as materials in this study. It was the first time that the ultrastructural characteristics of phloem tissues of source leaves were observed and compared in normal and weak light intensities using the transmission electron microscopy. Results showed that the average diameters of companion cells (CC) and sieve elements (SE) of all kinds of veins were bigger in normal than that in weak light intensity, indicating that light could influence the cell development and growth. Dense cytoplasm with abundant mitochondria, endoplasmic reticulums, multivesicular bodies, vesicles and plastids were observed in normal light intensity. On the contrary, CC with small vacuolar structures and few mitochondrias, endoplasmic reticulums were shown in weak light. Misalignment of grana thylakoid margins of nectarine leaves also was seen in weak light. The sieve pores of SEs were obstructed in weak light. Chloroplasts with numerous starch grains and few mitochondrias were noticed in the mesophyll cell (MES) surrounding the bundle sheath in weak light. The storage of starch grains appeared to result from an unbalance between photosynthate production and export of photosynthates. This observation provided a strong support to the point that most leaves export the most of assimilates in the light time. Plasmodesmal densities between SE/CC, CC/PP (phloem parenchyma cell), PP/PP and PP/BSC (bundle_sheath cell) decreased in weak light. Plasmodesmata were observed between CC/SE (NS) (nacreous_walled sieve element), PP/BSC in branch veins in normal light intensity, but not in weak light. Thus apoplasmic pathway may be the main mode of transport of assimilates in weak light, however symplasmic pathway may be the main mode of transport of assimilates in normal light intensity. These results demonstrated that the solar greenhouse nectarine trees could be adapted to the weak light via the ultrastructure variation of phloem tissues of the source leaves.展开更多
[Objective] This paper aimed to explore the genetic relationships among different types of Prunus domestica L. in Xinjiang. [Method] The inter-simple se- quence repeat （ISSR） markers were applied to analyze the gene...[Objective] This paper aimed to explore the genetic relationships among different types of Prunus domestica L. in Xinjiang. [Method] The inter-simple se- quence repeat （ISSR） markers were applied to analyze the genetic diversity and relationships among 30 individual plants from 5 types of P. domestica germpiasm resources in Xinjiang. [Result] A total of 317 bands were amplified by 16 selected IS- SR primers. Among the amplified bands, there were 246 polymorphic bands, accounting for 77.60% of the total. The Nei＇s gene diversity index （H） of individuals was 0.266 6. The average Shannon＇s information index （I） of individuals was 0.399 1. And most of the genetic similarity coefficients ranged from 0.555 2 to 0.996 8. It is indicated that the P. domestica germplasm resources have a certain genetic diversity in Xinjiang. The cluster analysis showed that, at the genetic similarity coefficient of 0.719, the 30 individuals were divided into 3 major groups, including a group of introduced European plum varieties, a group of Tacheng Smoked Plum and Tacheng Binzi and a group of Jiashi Smoked Plum and Wild European Plum. While at the genetic similarity coefficient of 0.949, Jiashi Smoked Plum and Wild European Plum were divided into two small groups. [Conclusion] There are very close genetic relationships between Jiashi Smoked Plum and Wild European Plum, and among the introduced European plum varieties, Tacheng Smoked Plum and Tacheng Binzi.展开更多
[Objective] This study aimed to investigate the photosynthetic characteristics of different Prunus domestica × armeniaca cultivars, thereby providing some theoretical basis for high-yield and high-quality cultiva...[Objective] This study aimed to investigate the photosynthetic characteristics of different Prunus domestica × armeniaca cultivars, thereby providing some theoretical basis for high-yield and high-quality cultivation of P. domestica × armeniaca. [Method] Total three cultivars（Konglongdan, Fengwei Huanghou and Weiwang）of P. domestica × armeniaca were selected as the test materials, and their leafphotosynthetic parameters were measured with Li-6400 portable photosynthesis system.The light response curve and CO_2 response curve were drawn. [Result] There were significant differences in photosynthetic rate among the three cultivars of P. domestica × armeniaca（P〈0.01）. The photosynthetic rate of Konglongdan [14.76 μmol/（m^2·s）]was highest. When the light intensity reached 1 800 μmol/（m^2·s）, the maximum net photosynthetic rate of Konglongdan was up to 27.27 μmol/（m^2·s）, and its apparent quantum yield reached the peak. Konglongdan showed lower light compensation point [43.069 μmol/（m^2·s）] and higher light saturation point [697.684 μmol/（m^2·s）].Weiwang showed the lowest CO2 compensation point（68.264 μmol/mol） and the highest carboxylic efficiency [0.072 μmol/（m^2·s）]. [Conclusion] Konglongdan showed the highest light utilization efficiency and photosynthetic potential, and it had the strongest utilization ability for weak light. The utilization ability of Weiwang for lowconcentration CO2 was strongest, followed by those of Fengwei Huanghou and Konglongdan.展开更多
Genetic relationships among Prunus mume var. pendula were studied by using AFLP markers. 18 accessions representing 14 cultivars of Prunus mume var. pendula were selected from the germplasm collection at the Research ...Genetic relationships among Prunus mume var. pendula were studied by using AFLP markers. 18 accessions representing 14 cultivars of Prunus mume var. pendula were selected from the germplasm collection at the Research Center of China Mei Flower. Seven Mse I-EcoR I AFLP primer combinations revealed 450 legible bands, and 269 of which were polymorphic markers. A similarity matrix was prepared using the simple matching coefficient of similarity and Neis (72) distance coefficient. A UPGMA dendrogram demonstrated the genetic relationships of the cultivars. The information given by AFLP markers was basically consistent with the morphological classification and the evolutionary history of the morphotypes, and roughly supported the new revised classification system for Chinese Mei Cultivars. But there were still several exceptions: 1) the Guhong Chuizhi inserted between the Tiaoxue Chuizhi and the Danfen Chuizhi; 2) the Wufu Chuizhi kept off the Pink Pendant Form, and the Moshan Chuizhi was removed from Viridiflora Pendant Form; 3) the Danbi Chuizhi and the Shuangbi Chuizhi of Viridiflora Pendant Form got together well but fell within the Pink Pendant Form.展开更多
[Objective] The aim of this study was to understand the flowering biological characteristics of different varieties Prunus domestica L. in Aksu Jiamu Experi- ment Station. [Method] Three varieties of plum as Prunus do...[Objective] The aim of this study was to understand the flowering biological characteristics of different varieties Prunus domestica L. in Aksu Jiamu Experi- ment Station. [Method] Three varieties of plum as Prunus domestica L. cv. ＇French＇, Prunus domestica L. cv. ＂Victoria＇ and Prunus domestica L. cv. ＂Stanley＇ were the research subjects. The phenophases of blooming, and the morphological and developmental characteristics of floral organs were observed. The amount and viability of pollen and the stigma receptivity were measured. [Result] Three varieties of plum all blossomed in early April, and the florescence had lasted for 9-10 d. The average number of anthers was 28.48%. The average number of pollen grains in each flower was 28 700.0. The pollen germination rate was 27.4%. 86.75% of the flowers were medium-styled or long-styled. There was no significant difference in the petal shape, color, anther number, calyx color, and flower ovary among the three varieties of plum. The stigma receptivity was strongest on the first day of flowering, and then dropped gradually. Therefore, the flowers of plum were manual pollinated on the first day of flowering. The duration time of stigma receptivity of Prunus domestica L. cv. ＇Victoria＇ was longer than the other cultivars, the duration time of stigma receptivity was about 5 d. [Conclusion] The pollen number and pollen germination rate of Prunus domestica L. cv. ＇Stanley＇ were higher than the other cultivars, which be considered the most suitable pollinizer for plum. In contrast, the complete flower rate of Prunus domestica L. cv. ＇French＇ and Prunus domestica L. cv. ＇Victoria＇ was relatively high, but the pollen number and pollen germination rate of Prunus domestica L. cv. ＇French＇ and Prunus domestica L. cv. ＇Victoria＇ were lower than Prunus domestica L. cv. ＇Stanley＇, which are not suitable to use as a pollinizer for plum.展开更多
[Objective]The aim was to study the effective factors on culturing Prunus salicina cv.Zaoshi embryos in vitro.[Method]Different age,culture medium and GA3 which affected culturing of prumssalicina embryos were discuss...[Objective]The aim was to study the effective factors on culturing Prunus salicina cv.Zaoshi embryos in vitro.[Method]Different age,culture medium and GA3 which affected culturing of prumssalicina embryos were discussed.[Result]The result showed that on the part of 26-41 d,after the blooming period,the embryos remained tiny and retained endosperms and showed no signs of change after having cultured for three generations.On the part of 48 d,after the blooming period,the endosperms had disappeared,the embryos kept growing until they filled the seed cavity;when they were planted on the MS culture medium,their survival rate reached 77%,in its first generation,the response of embryos was discernible.On the part of 65 d,after the blooming period,4.5% of their embryos grew into shoots on the MS culture medium;with the age of embryos growing,the survival rate of shoots increased until it reached 26% when the fruits went into ripeness;the embryos produced calli in their first generation of culturing.On the part of 65-83 d,after the blooming period,the embryos produced calli through more than 2-3 generations.On the part of 88 d,after the blooming period,the survival rate of shoots on the WPM culturing base doubled compared with that on the MS culturing base;on the same culture medium,the embryos were inhibited from growing into shoots when BA,KT or 2,4-D was added on to the culture medium.The survival rate of shoots was increased remarkably when the seeds were treated in 1 000 mg/L GA3.[Conclusion] This study provided experimental basis for the establishment of Prunus salicina cv.Zaoshi,embryos rescue techniques and cross breading.展开更多
A procedure for cutting Prunus humilis（Bge）. Sok was comprehensively studied in this paper. It was found that the key factors involved in this procession were medium, rooting accelerator, concentration of rooting ac...A procedure for cutting Prunus humilis（Bge）. Sok was comprehensively studied in this paper. It was found that the key factors involved in this procession were medium, rooting accelerator, concentration of rooting accelerator and type of shoot. The results showed that send was used as mediums; Treatment with 1 000 mg/L AST rooting powder No. 2 and semi-woody shoots were the optimal materials for cutting, and the rooting rate reached 88.1%. Anatomical study on rooting of Prunus hum#is（Bge）. Sok cutting has been carded out by the paraffin section method. The observation result shows that the adventitious root primordium of Prunus humili$（Bge）. Sok cutting belongs to the type of induced root primordium. The adventitiousroot primordium originates from the cross region of vascular cambium and pith rays.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.32570239)Key Technology Research and Development Program of Zhejiang Province(Grant No.2023C03138).
文摘Prunus spinulosa(2n=4x=32)is an evergreen species of significantmedicinal usage and ecological value.However,the lacking of a high-quality genome of P.spinulosa has obstructed further exploration of its ecological study and phylogenetic relationship of Prunus.In this study,we present the first haplotype-resolved genome assembly of Prunus s.l.subgenus Laurocerasus,the tetraploid genome of P.spinulosa was phased into 32 pseudochromosomes with 4 haplotypes,the genome size of each haplotype ranged from 249.82 Mb to 259.69 Mb,and N50 fluctuatedfrom 31.35 Mb to 33.25 Mb,the protein-coding genes vary from 21,272 to 22,668.Different evaluation methods showed that the P.spinulosa genome assembly has high quality of completeness,continuity and accuracy.Being the firstcomplete genome of P.spinulosa,it provides a valuable genetic resource for the Prunus tetraploid species database and supports further functional genomic study of this species.
基金funded by the National Natural Science Foundation of China(81573542)Shanxi Province's Traditional Chinese Medicine Technology Innovation Project(2100601).
文摘Objective:To explore a rapid and accurate method for evaluating the quality of Prunus mandshurica(Maxim.)Koehne(P.mandshurica,Ku Xing Ren)during rancidity using machine vision and learning.Methods:Sensory evaluation and chemometrics were used to classify P.mandshurica quality grades after rancidity.Chemical indicators of the P.mandshurica quality change were determined to verify the ob-tained grades and support the subsequent modeling.The International Commission on Illumination color space was used to extract the color features of the P.mandshurica.Discrimination and prediction models based on color features combined with multiple machine learning algorithms were established using 10-fold cross-validation and external test set validation.Results:The P.mandshurica rancidity samples were allocated to three quality grades.The Bayes net model based on powder color successfully identified the P.mandshurica at different grades with an accuracy of 88.89%and 100%using two validations,and the naive Bayes model based on section color achieved the same accuracy with an receiver operating characteristic area of 0.979.The instance-based k-nearest neighbors model based on powder color performed best in predicting the amygdalin content[R^(2)=0.9801,mean absolute error(MAE)=0.2071,root mean squared error(RMSE)=0.4170],followed by the random com-mittee model in predicting the acid value(R^(2)=0.9580,MAE=1.5121,RMSE=1.9099)and the random forest model in predicting the peroxide value(R^(2)=0.8857,MAE=0.0027,RMSE=0.0035).Conclusion:This study demonstrates that color digitization analysis is a potential method for rapidly evaluating the quality of P.mandshurica across the rancidity process,providing a new reference for the quality assessment of traditional Chinese medicines.
基金supported by National Basic Research Program of China (No. 2007CB109106)by the Zoology Key Subject Fund of Henan Province
文摘A semi-greenhouse study was conducted to understand the effects of soil burial depth on seed germination and seedling development. The seeds of wild apricot （Prunus armeniaca） were buried at the soil depths of 0-cm, 4-cm, 8-cm, and 12-cm, respectively, to simulate the seed hoarding behavior of rodents in the field. The results revealed that the rates of seed germination and established seedlings from buried seeds were both the highest in 4-cm burial depth group, and then decreased with increasing soil depth. The number of rotten seeds increased in deeper burial depth. It is unfavourable for seed germination at 0-cm burial depth （i.e., seeds were laid on soil surface）. There was insignificant effect of burial depth on growth of established seedlings. The results from this study indicated that proper burial depth in soil would be helpful for the seed germination and seedling growth. The seedlings derived from buried seeds at shallower depth （4 cm） in this research have advantage in their early development.
文摘Polygalacturonase (PG,EC3.2.1.15) is the key cell wall hydrolase in fruit ripening. The identification and characterization of a full length cDNA (pMT18) encoding for PG from Feicheng peach (Prunus persica (L.) Batsch cv. Feicheng) is described. The pMT18 clone is 1188 bp in length, with an open reading frame of 393 amino acids. The homology and phylogenetic analyses indicate a remarkable similarity between peach PG and other ripening related PG. And seven consensus sequences have revealed in peach PG compared to the PG from other plants. However, the profound divergence with other PG and the unique structure features suggest that peach PG probably belongs to a new evolutionary class. In RT PCR analysis, pMT18 related RNA was undetectable in leaves, and was much abundant in ripe fruits. The ripening specific expression pattern of this cDNA will be useful in investigating the roles of PG in fruit ripening and developing a transgenic peach with the improved post harvesting quality in the future.
基金This study was supported by Science and Technology Program of Heilongjiang Province (GC01KB213), and the Quick Response of Basic Research Supporting Program (2001CCB00600)
文摘One-year-old seedlings of Amur maple (Acer ginnala Maxim), Ussurian pear (Pyrus ussuriensis Maxim) and David peach (Prunus davidiana Carr) were planted in pots in greenhouse and treated with four different soil moisture contents (75.0%, 61.1%, 46.4% and 35.4%). The results showed that net photosynthesis rate (NPR), transpiration rate (TR) and stomatal conductance (Sc) of seedlings of the three species decreased with the decease of soil moisture content, and Amur maple seedlings had the greatest change in those physiological indices, followed by Ussurian pear, David peach. Amur maple and Ussurian pear seedlings also presented a decrease tendency in water use efficiency (WUE) under lower soil moisture content, whereas this was reversed for David peach. Under water stress the biomass allocation to seedling root had a significant increase for all the experimental species. As to root/shoot ratio, Amur maple seedlings had the biggest increase, while David peach had the smallest increase. The leaf plasticity of Amur maple seedlings was greater, the leaf size and total leaf area decreased significantly as the stress was intensified. No significant change of leaf size and total leaf area was found in seedlings of Ussurian pear and David peach. It was concluded that Amur maple was more tolerant to soil moisture stress in comparison with David peach and Ussurian pear.
基金Supported by Science and Technology Planning Project of Guang-dong Province (2007A020300002-6)~~
文摘Using ISSR technique to analyze the diversity and genetic relationship of germplasm resources in 39 Prunus mume Sibe. et Zucc., the result showed that 10 primers were screened with high resolution from 51 primers, 120 fragments were amplified, of which 98 were polymorphic loci, accounting for 81.67% of total. Tested materials were divided into 3 classes, as was fundamentally accorded with the traditional classification base on horticulture. There was no obvious difference in geographic relationship among the clustering results.
基金Supported by Highly-qualified and Highly-efficient Cultivation Technology Demonstration adn Promotion of Apricot and Plum~~
文摘[Objective] The aims of this study were to understand the flowering biological characteristics of Prunus domestica ×armeniaca and analyze its differences with other apricot varieties and plum varieties. [Method] Taking three varieties of Prunus domestica ×armeniaca as the research object, the phenophase of blooming,morphology characteristics of floral organs and development characteristics of floral organs were observed, pollen amount, pollen viability and stigma receptivity were measured. [Result] The results showed that three varieties of Prunus domestica×armeniaca were in blossom in early April, the florescence were relatively late for 8-12 d comparing with the varieties of apricot, the florescence were relatively similar to the P. domestica L. Morphology characteristics of floral organs of three varieties of Prunus domestica×armeniaca had few differences, were relatively similar to two varieties of plum, and they all had high percent of middle-style and long-style flower.The pollen amount of three varieties of Prunus domestica ×armeniaca had a little differences comparing with two varieties of plum, but were lower than three varieties of apricot. There were some differences in pollen viability, the germination percentage of pollen of ‘Konglongdan＇ was relatively high, was 51.6%; The pollen amount of ‘Fengweihuanghou＇ was relatively high, was 880.8 per anther; ‘Weidi＇ had fewer pollen amount, and the pollen viability was just 2.1%; Three varieties of Prunus domestica ×armeniaca all had strong stigma receptivity on the bloom day, and the stigma receptivity continued exist until the day that 5 days after blooming. [Conclusion] The florescence of three varieties of Prunus domestica ×armeniaca were relatively concentrated, and the rate of pistil abortion was lowest, the variation intensity of stigma receptivity and sustainable time of three varieties of Prunus domestica×armeniaca almost had a little differences comparing with a variety of plum, but slightly more than three varieties of apricot.
文摘Leaves from three_year_old solar greenhouse nectarine trees ( Prunus persica L. var. nectarina Ait. “Zao Hong Yan”) were used as materials in this study. It was the first time that the ultrastructural characteristics of phloem tissues of source leaves were observed and compared in normal and weak light intensities using the transmission electron microscopy. Results showed that the average diameters of companion cells (CC) and sieve elements (SE) of all kinds of veins were bigger in normal than that in weak light intensity, indicating that light could influence the cell development and growth. Dense cytoplasm with abundant mitochondria, endoplasmic reticulums, multivesicular bodies, vesicles and plastids were observed in normal light intensity. On the contrary, CC with small vacuolar structures and few mitochondrias, endoplasmic reticulums were shown in weak light. Misalignment of grana thylakoid margins of nectarine leaves also was seen in weak light. The sieve pores of SEs were obstructed in weak light. Chloroplasts with numerous starch grains and few mitochondrias were noticed in the mesophyll cell (MES) surrounding the bundle sheath in weak light. The storage of starch grains appeared to result from an unbalance between photosynthate production and export of photosynthates. This observation provided a strong support to the point that most leaves export the most of assimilates in the light time. Plasmodesmal densities between SE/CC, CC/PP (phloem parenchyma cell), PP/PP and PP/BSC (bundle_sheath cell) decreased in weak light. Plasmodesmata were observed between CC/SE (NS) (nacreous_walled sieve element), PP/BSC in branch veins in normal light intensity, but not in weak light. Thus apoplasmic pathway may be the main mode of transport of assimilates in weak light, however symplasmic pathway may be the main mode of transport of assimilates in normal light intensity. These results demonstrated that the solar greenhouse nectarine trees could be adapted to the weak light via the ultrastructure variation of phloem tissues of the source leaves.
文摘[Objective] This paper aimed to explore the genetic relationships among different types of Prunus domestica L. in Xinjiang. [Method] The inter-simple se- quence repeat （ISSR） markers were applied to analyze the genetic diversity and relationships among 30 individual plants from 5 types of P. domestica germpiasm resources in Xinjiang. [Result] A total of 317 bands were amplified by 16 selected IS- SR primers. Among the amplified bands, there were 246 polymorphic bands, accounting for 77.60% of the total. The Nei＇s gene diversity index （H） of individuals was 0.266 6. The average Shannon＇s information index （I） of individuals was 0.399 1. And most of the genetic similarity coefficients ranged from 0.555 2 to 0.996 8. It is indicated that the P. domestica germplasm resources have a certain genetic diversity in Xinjiang. The cluster analysis showed that, at the genetic similarity coefficient of 0.719, the 30 individuals were divided into 3 major groups, including a group of introduced European plum varieties, a group of Tacheng Smoked Plum and Tacheng Binzi and a group of Jiashi Smoked Plum and Wild European Plum. While at the genetic similarity coefficient of 0.949, Jiashi Smoked Plum and Wild European Plum were divided into two small groups. [Conclusion] There are very close genetic relationships between Jiashi Smoked Plum and Wild European Plum, and among the introduced European plum varieties, Tacheng Smoked Plum and Tacheng Binzi.
基金Supported by Demonstration and Extension Project for High-quality and High-efficiency Cultivation Techniques of Prunus domestica×armeniaca(ZYLYKJTG2015020)~~
文摘[Objective] This study aimed to investigate the photosynthetic characteristics of different Prunus domestica × armeniaca cultivars, thereby providing some theoretical basis for high-yield and high-quality cultivation of P. domestica × armeniaca. [Method] Total three cultivars（Konglongdan, Fengwei Huanghou and Weiwang）of P. domestica × armeniaca were selected as the test materials, and their leafphotosynthetic parameters were measured with Li-6400 portable photosynthesis system.The light response curve and CO_2 response curve were drawn. [Result] There were significant differences in photosynthetic rate among the three cultivars of P. domestica × armeniaca（P〈0.01）. The photosynthetic rate of Konglongdan [14.76 μmol/（m^2·s）]was highest. When the light intensity reached 1 800 μmol/（m^2·s）, the maximum net photosynthetic rate of Konglongdan was up to 27.27 μmol/（m^2·s）, and its apparent quantum yield reached the peak. Konglongdan showed lower light compensation point [43.069 μmol/（m^2·s）] and higher light saturation point [697.684 μmol/（m^2·s）].Weiwang showed the lowest CO2 compensation point（68.264 μmol/mol） and the highest carboxylic efficiency [0.072 μmol/（m^2·s）]. [Conclusion] Konglongdan showed the highest light utilization efficiency and photosynthetic potential, and it had the strongest utilization ability for weak light. The utilization ability of Weiwang for lowconcentration CO2 was strongest, followed by those of Fengwei Huanghou and Konglongdan.
基金the Natural Science Foundation of Hubei Province P. R. China (Grant No. 2001ABB118)
文摘Genetic relationships among Prunus mume var. pendula were studied by using AFLP markers. 18 accessions representing 14 cultivars of Prunus mume var. pendula were selected from the germplasm collection at the Research Center of China Mei Flower. Seven Mse I-EcoR I AFLP primer combinations revealed 450 legible bands, and 269 of which were polymorphic markers. A similarity matrix was prepared using the simple matching coefficient of similarity and Neis (72) distance coefficient. A UPGMA dendrogram demonstrated the genetic relationships of the cultivars. The information given by AFLP markers was basically consistent with the morphological classification and the evolutionary history of the morphotypes, and roughly supported the new revised classification system for Chinese Mei Cultivars. But there were still several exceptions: 1) the Guhong Chuizhi inserted between the Tiaoxue Chuizhi and the Danfen Chuizhi; 2) the Wufu Chuizhi kept off the Pink Pendant Form, and the Moshan Chuizhi was removed from Viridiflora Pendant Form; 3) the Danbi Chuizhi and the Shuangbi Chuizhi of Viridiflora Pendant Form got together well but fell within the Pink Pendant Form.
基金Supported by High-quality and Highly-efficient Cultivation Technology Promotion of Prunus simonii~~
文摘[Objective] The aim of this study was to understand the flowering biological characteristics of different varieties Prunus domestica L. in Aksu Jiamu Experi- ment Station. [Method] Three varieties of plum as Prunus domestica L. cv. ＇French＇, Prunus domestica L. cv. ＂Victoria＇ and Prunus domestica L. cv. ＂Stanley＇ were the research subjects. The phenophases of blooming, and the morphological and developmental characteristics of floral organs were observed. The amount and viability of pollen and the stigma receptivity were measured. [Result] Three varieties of plum all blossomed in early April, and the florescence had lasted for 9-10 d. The average number of anthers was 28.48%. The average number of pollen grains in each flower was 28 700.0. The pollen germination rate was 27.4%. 86.75% of the flowers were medium-styled or long-styled. There was no significant difference in the petal shape, color, anther number, calyx color, and flower ovary among the three varieties of plum. The stigma receptivity was strongest on the first day of flowering, and then dropped gradually. Therefore, the flowers of plum were manual pollinated on the first day of flowering. The duration time of stigma receptivity of Prunus domestica L. cv. ＇Victoria＇ was longer than the other cultivars, the duration time of stigma receptivity was about 5 d. [Conclusion] The pollen number and pollen germination rate of Prunus domestica L. cv. ＇Stanley＇ were higher than the other cultivars, which be considered the most suitable pollinizer for plum. In contrast, the complete flower rate of Prunus domestica L. cv. ＇French＇ and Prunus domestica L. cv. ＇Victoria＇ was relatively high, but the pollen number and pollen germination rate of Prunus domestica L. cv. ＇French＇ and Prunus domestica L. cv. ＇Victoria＇ were lower than Prunus domestica L. cv. ＇Stanley＇, which are not suitable to use as a pollinizer for plum.
基金Support by Major Science and Technology Projects of Guangdong Province (2004A20102002)~~
文摘[Objective]The aim was to study the effective factors on culturing Prunus salicina cv.Zaoshi embryos in vitro.[Method]Different age,culture medium and GA3 which affected culturing of prumssalicina embryos were discussed.[Result]The result showed that on the part of 26-41 d,after the blooming period,the embryos remained tiny and retained endosperms and showed no signs of change after having cultured for three generations.On the part of 48 d,after the blooming period,the endosperms had disappeared,the embryos kept growing until they filled the seed cavity;when they were planted on the MS culture medium,their survival rate reached 77%,in its first generation,the response of embryos was discernible.On the part of 65 d,after the blooming period,4.5% of their embryos grew into shoots on the MS culture medium;with the age of embryos growing,the survival rate of shoots increased until it reached 26% when the fruits went into ripeness;the embryos produced calli in their first generation of culturing.On the part of 65-83 d,after the blooming period,the embryos produced calli through more than 2-3 generations.On the part of 88 d,after the blooming period,the survival rate of shoots on the WPM culturing base doubled compared with that on the MS culturing base;on the same culture medium,the embryos were inhibited from growing into shoots when BA,KT or 2,4-D was added on to the culture medium.The survival rate of shoots was increased remarkably when the seeds were treated in 1 000 mg/L GA3.[Conclusion] This study provided experimental basis for the establishment of Prunus salicina cv.Zaoshi,embryos rescue techniques and cross breading.
文摘A procedure for cutting Prunus humilis（Bge）. Sok was comprehensively studied in this paper. It was found that the key factors involved in this procession were medium, rooting accelerator, concentration of rooting accelerator and type of shoot. The results showed that send was used as mediums; Treatment with 1 000 mg/L AST rooting powder No. 2 and semi-woody shoots were the optimal materials for cutting, and the rooting rate reached 88.1%. Anatomical study on rooting of Prunus hum#is（Bge）. Sok cutting has been carded out by the paraffin section method. The observation result shows that the adventitious root primordium of Prunus humili$（Bge）. Sok cutting belongs to the type of induced root primordium. The adventitiousroot primordium originates from the cross region of vascular cambium and pith rays.
