Pruni Semen,the seed of several unique Prunus plants,is a traditional purgative herbal material.To determine the authentic sources of Pruni Semen,46 samples from four species were collected and analyzed.Ten compounds ...Pruni Semen,the seed of several unique Prunus plants,is a traditional purgative herbal material.To determine the authentic sources of Pruni Semen,46 samples from four species were collected and analyzed.Ten compounds including multiflorin A(Mul A),a notable purative compound,were isolated and identified by chemical separation and nuclear magnetic resonance spectroscopy.Seventy-six communal components were identified by ultra-high performance liquid chromatography with linear ion trap-quadrupole Orbitrap mass spectrometry,and acetyl flavonoid glycosides were recognized as characteristic constituents.The flavonoids were distributed in the seed coat and cyanogenic glycosides in the kernel.Based on this,methods for identifying Pruni Semen from different sources were established using chemical fingerprinting,quantitative analysis of the eight principal compounds,hierarchical cluster analysis,principal component analysis,and orthogonal partial least squares discriminant analysis.The results showed that the samples were divided into two categories:one is the small seeds from Prunus humilis(Ph)and Prunus japonica(Pj),and the other is the big seeds from Prunus pedunculata(Pp)and Prunus triloba(Pt).The average content of Mul A was 3.02,6.93,0.40,and 0.29 mg/g,while the average content of amygdalin was 18.5,17.7,31.5,and 30.9 mg/g in Ph,Pj,Pp,and Pt,respectively.All the above information suggests that small seeds might be superior sources of Pruni Semen.This is the first comprehensive report on the identification of chemical components in Pruni Semen from different species.展开更多
Bacterial spot caused by Xanthomonas arboricola pv. pruni (Xap) is considered as a major problem in peach orchards. Copper and antibiotics are used to control, and biocontrol should be a new alternative with low envir...Bacterial spot caused by Xanthomonas arboricola pv. pruni (Xap) is considered as a major problem in peach orchards. Copper and antibiotics are used to control, and biocontrol should be a new alternative with low environment impact. The objective was evaluated by the antibiotic activity of the metabolite produced by Pseudomonas aeruginosa LV strain. The free cells supernatant was fractionated with a serial organic solvent with crescent polarity and a dichloromethane phase was concentrated and lyophilised, and after was fractionated using vacuum liquid chromatography. The antibiotic activity of the F3 fraction containing an organometallic compound was tested on Xap in vitro and in a greenhouse conditions. Plants were sprayed with F3 before or after Xap infection and the results showed changes in exopolysaccharides and cell morphology. The F3 concentration of 450 μg·mL-1 was more effective. The results showed that F3 fraction could be a new alternative to control bacterial spot.展开更多
目的:通过网络药理学及分子对接技术探究白芷-补骨脂-乌梅治疗白癜风-高尿酸血症的作用机制。方法:通用中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)、...目的:通过网络药理学及分子对接技术探究白芷-补骨脂-乌梅治疗白癜风-高尿酸血症的作用机制。方法:通用中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)、中医药综合数据库(Traditional Chinese Medicine Integrative Database,TCMID)、本草组鉴(A high-throughput experiment-and reference-guided database of traditional Chinese medicine,HERB)等数据库检索白芷-补骨脂-乌梅的主要活性成分以及相关靶点,采用通用蛋白质资源数据库(Universal Protein Resourse,UniProt)进行靶点蛋白与基因名的转换。在基因卡片(The Human Gene Database,GeneCards)、在线人类孟德尔遗传数据库(Online Mendelian Inheritance in Man,OMIM)及疾病基因网络数据库(Disease Gene Network,Disgenet)检索白癜风和高尿酸血症相关靶点,借助韦恩图查找白芷-补骨脂-乌梅与白癜风-高尿酸血症相关联的核心靶点基因,对核心靶点基因进行蛋白质-蛋白质相互作用(proteinprotein interatction,PPI)网络分析、基因本体(gene ontology,GO)功能注释和京都基因与基因组数据库(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析。采用AutoDock 1.5.7版进行分子对接,并应用PyMOL分子图形系统(PyMOL Molecular Graphics System,PyMOL)2.5.2软件对对接结果进行可视化分析。结果:通过网络药理学筛选获得白芷-补骨脂-乌梅有效成分47种,关键靶点基因50个,其中以槲皮素(quercetin)、山柰酚(kaempferol)等为重要活性成分,以肿瘤坏死因子(tumor necrosis factor,TNF)、白细胞介素-6(interleukin-6,IL-6)、白细胞介素-1β(interleukin-1β,IL-1β)等靶点作用较为突出。KEGG通路富集分析结果提示白芷-补骨脂-乌梅药对可能通过调节晚期糖基化终末产物-晚期糖基化终末产物受体(advanced glycation end products-receptor for advanced glycation end products,AGE-RAGE)信号通路、病毒感染信号通路等发挥治疗作用。分子对接结果显示槲皮素、山柰酚等活性成分与TNF、IL-6、IL-1β等核心靶点之间的结合力较稳定。结论:白芷-补骨脂-乌梅药对能够从多基因、多靶点、多途径治疗白癜风-高尿酸血症,为进一步的临床药物研究和治疗途径提供了数据支持。展开更多
基金sponsored by the Key Research and Development Programs in the Ningxia Hui Autonomous Region,China(Grant No.:2020BBF02027)Beijing Natural Science Foundation(Grant No.:5212014).
文摘Pruni Semen,the seed of several unique Prunus plants,is a traditional purgative herbal material.To determine the authentic sources of Pruni Semen,46 samples from four species were collected and analyzed.Ten compounds including multiflorin A(Mul A),a notable purative compound,were isolated and identified by chemical separation and nuclear magnetic resonance spectroscopy.Seventy-six communal components were identified by ultra-high performance liquid chromatography with linear ion trap-quadrupole Orbitrap mass spectrometry,and acetyl flavonoid glycosides were recognized as characteristic constituents.The flavonoids were distributed in the seed coat and cyanogenic glycosides in the kernel.Based on this,methods for identifying Pruni Semen from different sources were established using chemical fingerprinting,quantitative analysis of the eight principal compounds,hierarchical cluster analysis,principal component analysis,and orthogonal partial least squares discriminant analysis.The results showed that the samples were divided into two categories:one is the small seeds from Prunus humilis(Ph)and Prunus japonica(Pj),and the other is the big seeds from Prunus pedunculata(Pp)and Prunus triloba(Pt).The average content of Mul A was 3.02,6.93,0.40,and 0.29 mg/g,while the average content of amygdalin was 18.5,17.7,31.5,and 30.9 mg/g in Ph,Pj,Pp,and Pt,respectively.All the above information suggests that small seeds might be superior sources of Pruni Semen.This is the first comprehensive report on the identification of chemical components in Pruni Semen from different species.
文摘Bacterial spot caused by Xanthomonas arboricola pv. pruni (Xap) is considered as a major problem in peach orchards. Copper and antibiotics are used to control, and biocontrol should be a new alternative with low environment impact. The objective was evaluated by the antibiotic activity of the metabolite produced by Pseudomonas aeruginosa LV strain. The free cells supernatant was fractionated with a serial organic solvent with crescent polarity and a dichloromethane phase was concentrated and lyophilised, and after was fractionated using vacuum liquid chromatography. The antibiotic activity of the F3 fraction containing an organometallic compound was tested on Xap in vitro and in a greenhouse conditions. Plants were sprayed with F3 before or after Xap infection and the results showed changes in exopolysaccharides and cell morphology. The F3 concentration of 450 μg·mL-1 was more effective. The results showed that F3 fraction could be a new alternative to control bacterial spot.
文摘目的:通过网络药理学及分子对接技术探究白芷-补骨脂-乌梅治疗白癜风-高尿酸血症的作用机制。方法:通用中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)、中医药综合数据库(Traditional Chinese Medicine Integrative Database,TCMID)、本草组鉴(A high-throughput experiment-and reference-guided database of traditional Chinese medicine,HERB)等数据库检索白芷-补骨脂-乌梅的主要活性成分以及相关靶点,采用通用蛋白质资源数据库(Universal Protein Resourse,UniProt)进行靶点蛋白与基因名的转换。在基因卡片(The Human Gene Database,GeneCards)、在线人类孟德尔遗传数据库(Online Mendelian Inheritance in Man,OMIM)及疾病基因网络数据库(Disease Gene Network,Disgenet)检索白癜风和高尿酸血症相关靶点,借助韦恩图查找白芷-补骨脂-乌梅与白癜风-高尿酸血症相关联的核心靶点基因,对核心靶点基因进行蛋白质-蛋白质相互作用(proteinprotein interatction,PPI)网络分析、基因本体(gene ontology,GO)功能注释和京都基因与基因组数据库(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析。采用AutoDock 1.5.7版进行分子对接,并应用PyMOL分子图形系统(PyMOL Molecular Graphics System,PyMOL)2.5.2软件对对接结果进行可视化分析。结果:通过网络药理学筛选获得白芷-补骨脂-乌梅有效成分47种,关键靶点基因50个,其中以槲皮素(quercetin)、山柰酚(kaempferol)等为重要活性成分,以肿瘤坏死因子(tumor necrosis factor,TNF)、白细胞介素-6(interleukin-6,IL-6)、白细胞介素-1β(interleukin-1β,IL-1β)等靶点作用较为突出。KEGG通路富集分析结果提示白芷-补骨脂-乌梅药对可能通过调节晚期糖基化终末产物-晚期糖基化终末产物受体(advanced glycation end products-receptor for advanced glycation end products,AGE-RAGE)信号通路、病毒感染信号通路等发挥治疗作用。分子对接结果显示槲皮素、山柰酚等活性成分与TNF、IL-6、IL-1β等核心靶点之间的结合力较稳定。结论:白芷-补骨脂-乌梅药对能够从多基因、多靶点、多途径治疗白癜风-高尿酸血症,为进一步的临床药物研究和治疗途径提供了数据支持。
