Mesangial cell proliferation is an early pathological indicator of diabetic nephropathy(DN).Growing evidence highlights the pivotal role of paired-related homeobox 1(Prrx1),a key regulator of cellular proliferation an...Mesangial cell proliferation is an early pathological indicator of diabetic nephropathy(DN).Growing evidence highlights the pivotal role of paired-related homeobox 1(Prrx1),a key regulator of cellular proliferation and tissue differentiation,in various disease pathogenesis.Notably,Prrx1 is highly expressed in mesangial cells under DN conditions.Both in vitro and in vivo studies have demonstrated that Prrx1 overexpression promotes mesangial cell proliferation and contributes to renal fibrosis in db/m mice.Conversely,Prrx1 knockdown markedly suppresses hyperglycemia-induced mesangial cell prolif-eration and mitigates renal fibrosis in db/db mice.Mechanistically,Prrx1 directly interacts with the Yes-associated protein 1(YAP)promoter,leading to the upregulation of YAP expression.This upregulation promotes mesangial cell proliferation and exacerbates renal fibrosis.These findings emphasize the crucial role of Prrx1 upregulation in high glucose-induced mesangial cell proliferation,ultimately leading to renal fibrosis in DN.Therefore,targeting Prrx1 to downregulate its expression presents a promising therapeutic strategy for treating renal fibrosis associated with DN.展开更多
转录因子配对相关同源框1(paired related homeobox 1,Prrx1)作为一种间充质细胞的标志物,不仅参与胚胎时期的骨发育过程,亦在机体成年后骨稳态的调节与损伤后的修复过程中发挥作用。Prrx1+细胞是间充质细胞的一种亚群,作为成骨的前体...转录因子配对相关同源框1(paired related homeobox 1,Prrx1)作为一种间充质细胞的标志物,不仅参与胚胎时期的骨发育过程,亦在机体成年后骨稳态的调节与损伤后的修复过程中发挥作用。Prrx1+细胞是间充质细胞的一种亚群,作为成骨的前体细胞参与骨骼的发育与形成。本文对Prrx1及Prrx1+细胞在骨发育及骨改建中的作用及影响进行综述。展开更多
Guided bone regeneration(GBR)is widely applied in implant dentistry,employing barrier membranes to create an osteogenic space by preventing gingival tissue ingrowth.However,this method does not enhance the oste-ogenic...Guided bone regeneration(GBR)is widely applied in implant dentistry,employing barrier membranes to create an osteogenic space by preventing gingival tissue ingrowth.However,this method does not enhance the oste-ogenic capacity of osteoblasts,limiting sufficient bone volume in larger defects.Inspired by axolotl limb regeneration,abundant soft tissue-derived stem cells mobilized to the defect may facilitate comprehensive osteogenesis within a BMP-2-enriched environment.We developed a biomimetic channel system(BCS)to pro-mote alveolar bone regeneration,using channel structures to activate gingival-derived stem cells under a BMP-2-enriched biological barrier.In a cell-tracing mouse model,Prrx1+stem cells demonstrated a critical role in BMP-2-induced subcutaneous osteogenesis.Sequencing and histological analyses revealed that channel structures significantly enhance soft tissue cell proliferation and migration.Attributable to the biological barrier,BCS applications markedly improved bone formation in beagle mandibular defects.These results suggest a novel osteoinductive strategy for alveolar bone regeneration that functions without a traditional barrier membrane.展开更多
基金the National Natural Science Foundation of China(Grant Nos.:82273987,81973377,82073906)PostgraduateResearch Practice Innovation Program of Jiangsu Province(Grant No.:KYCX23_2994)+1 种基金the data provided by the GEO database,and the single cell analysis provided by the KIT platform.KOBAs for its contribution to the data analysis.
文摘Mesangial cell proliferation is an early pathological indicator of diabetic nephropathy(DN).Growing evidence highlights the pivotal role of paired-related homeobox 1(Prrx1),a key regulator of cellular proliferation and tissue differentiation,in various disease pathogenesis.Notably,Prrx1 is highly expressed in mesangial cells under DN conditions.Both in vitro and in vivo studies have demonstrated that Prrx1 overexpression promotes mesangial cell proliferation and contributes to renal fibrosis in db/m mice.Conversely,Prrx1 knockdown markedly suppresses hyperglycemia-induced mesangial cell prolif-eration and mitigates renal fibrosis in db/db mice.Mechanistically,Prrx1 directly interacts with the Yes-associated protein 1(YAP)promoter,leading to the upregulation of YAP expression.This upregulation promotes mesangial cell proliferation and exacerbates renal fibrosis.These findings emphasize the crucial role of Prrx1 upregulation in high glucose-induced mesangial cell proliferation,ultimately leading to renal fibrosis in DN.Therefore,targeting Prrx1 to downregulate its expression presents a promising therapeutic strategy for treating renal fibrosis associated with DN.
文摘转录因子配对相关同源框1(paired related homeobox 1,Prrx1)作为一种间充质细胞的标志物,不仅参与胚胎时期的骨发育过程,亦在机体成年后骨稳态的调节与损伤后的修复过程中发挥作用。Prrx1+细胞是间充质细胞的一种亚群,作为成骨的前体细胞参与骨骼的发育与形成。本文对Prrx1及Prrx1+细胞在骨发育及骨改建中的作用及影响进行综述。
基金supported by the National Natural Science Foundation of China(Grant Nos.82022015,32071361,82370923,81921002 and 82100966)the Fundamental Research Funds for the Central Universities(YG2023ZD16)the Health Discipline Con-struction Project in Pudong New Area(No.PWYts2021-20).
文摘Guided bone regeneration(GBR)is widely applied in implant dentistry,employing barrier membranes to create an osteogenic space by preventing gingival tissue ingrowth.However,this method does not enhance the oste-ogenic capacity of osteoblasts,limiting sufficient bone volume in larger defects.Inspired by axolotl limb regeneration,abundant soft tissue-derived stem cells mobilized to the defect may facilitate comprehensive osteogenesis within a BMP-2-enriched environment.We developed a biomimetic channel system(BCS)to pro-mote alveolar bone regeneration,using channel structures to activate gingival-derived stem cells under a BMP-2-enriched biological barrier.In a cell-tracing mouse model,Prrx1+stem cells demonstrated a critical role in BMP-2-induced subcutaneous osteogenesis.Sequencing and histological analyses revealed that channel structures significantly enhance soft tissue cell proliferation and migration.Attributable to the biological barrier,BCS applications markedly improved bone formation in beagle mandibular defects.These results suggest a novel osteoinductive strategy for alveolar bone regeneration that functions without a traditional barrier membrane.
