目的旨在通过生物信息学分析结合实验验证,探讨PCBP1(Poly(rC)-Binding Protein 1)在胃癌组织中的表达特征及其临床意义,对其与铁死亡主要调控因子STUB1(STIP1 Homology and U-Box Containing Protein 1)的关系研究。并通过免疫组化实...目的旨在通过生物信息学分析结合实验验证,探讨PCBP1(Poly(rC)-Binding Protein 1)在胃癌组织中的表达特征及其临床意义,对其与铁死亡主要调控因子STUB1(STIP1 Homology and U-Box Containing Protein 1)的关系研究。并通过免疫组化实验验证PCBP1与STUB1在胃癌中的表达模式及其与临床病理特征的关系。为新型胃癌靶向治疗策略提供重要的理论支撑和潜在的干预靶点。方法从TIMER 2.0在线分析网站中获取PCBP1在胃癌及癌旁组织的基因表达数据。利用癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库中胃癌数据(STAD)进行KEGG通路富集分析,并揭示其潜在作用机制;在铁死亡调控通路中找出主要调控因子STUB1。随后,采用免疫组化法检测33例胃癌组织及对应癌旁组织中PCBP1和STUB1的表达情况。将所收集的病例依据不同的分化程度、年龄、性别、肿瘤浸润深度、TNM分期及病理学形态进行分组,观察二者的阳性表达率,采用χ^(2)检验分析两者之间及其与临床和病理特征间的相关性,进一步探讨PCBP1和STUB1与胃癌恶性程度的关系。结果免疫组化结果显示PCBP1在癌组织中的阳性表达率为69.7%,明显高于癌旁组织中的阳性表达率48.5%;STUB1在癌组织中的阳性表达率为39.4%,低于癌旁组织中的阳性表达率54.5%,两者差异均有统计学意义(P<0.05)。PCBP1的阳性表达率与肿瘤分化程度、淋巴结转移及Lauren分型有相关性(P<0.05);与患者的年龄、性别、浸润深度、临床分期、神经浸润、脉管侵犯无相关性(P>0.05)。STUB1的阳性表达率与肿瘤分化程度、浸润深度、淋巴结转移及Lauren分型有相关性(P<0.05),但与患者的年龄、性别、临床分期、神经浸润、脉管侵犯无相关性(P>0.05)。PCBP1(癌)与STUB1(癌)的Spearman相关系数为-0.413,P为0.017,表明两者之间存在显著的负相关性。结论PCBP1可通过调控铁死亡通路中主要调控因子STUB1参与胃癌的恶性进展。为胃癌的分子机制探索及潜在治疗靶点提供了新的理论依据。展开更多
With the rapid development of web3.0 applications,the volume of data sharing is increasing,the inefficiency of big data file sharing and the problem of data privacy leakage are becoming more and more prominent,and the...With the rapid development of web3.0 applications,the volume of data sharing is increasing,the inefficiency of big data file sharing and the problem of data privacy leakage are becoming more and more prominent,and the existing data sharing schemes have been difficult to meet the growing demand for data sharing,this paper aims at exploring a secure,efficient and privacy-protecting data sharing scheme under web3.0 applications.Specifically,this paper adopts interplanetary file system(IPFS)technology to realize the storage of large data files to solve the problem of blockchain storage capacity limitation,and utilizes ciphertext policy attribute-based encryption(CP-ABE)and proxy re-encryption(PRE)technology to realize secure multi-party sharing and finegrained access control of data.This paper provides the detailed algorithm design and implementation of data sharing phases and processes,and analyzes the algorithms from the perspectives of security,privacy protection,and performance.展开更多
Objectives This study aimed to clarify the relationship between the content of proxy decision-making made by families of patients with malignant brain tumors regarding treatment policies and daily care and the cues le...Objectives This study aimed to clarify the relationship between the content of proxy decision-making made by families of patients with malignant brain tumors regarding treatment policies and daily care and the cues leading to those decisions.Methods Semi-structured personal interviews were used to collect data.Seven family members of patients with malignant brain tumors were selected to participate in the study by purposive sampling method from June to August 2022 in the Patient Family Association of Japan.Responses were content analyzed to explore the relationship between the content of decisions regarding“treatment policies”and“daily care”and the cues influencing those decisions.Semi-structured interviews were analyzed by using thematic analysis.Results The contents of proxy decisions regarding“treatment policies”included implementation,interruption,and termination of initial treatments,free medical treatments,use of respirators,and end-of-life sedation and included six cues:treatment policies suggested by the primary physician,information and knowledge about the disease and treatment obtained by the family from limited resources,perceived life threat from symptom worsening,words and reactions from the patient regarding treatment,patient’s personality and way of life inferred from their treatment preferences,family’s thoughts and values hoping for better treatment for the patient.Decisions for“daily care”included meal content and methods,excretion,mobility,maintaining cleanliness,rehabilitation,continuation or resignation from work,treatment settings(outpatient or inpatient),and ways to spend time outside and included seven cues:words and thoughts from the patient about their way of life,patient’s reactions and life history inferred from their preferred way of living,things the patient can do to maintain daily life and roles,awareness of the increasing inability to do things in daily life,family’s underlying thoughts and values about how to spend the remaining time,approval from family members regarding the care setting,advice from medical professionals on living at home.Conclusions For“treatment policies,”guidelines from medical professionals were a key cue,while for“daily care,”the small signs from the patients in their daily lives served as cues for proxy decision-making.This may be due to the lack of information available to families and the limited time available for discussion with the patient.Families of patients with malignant brain tumors repeatedly use multiple cues to make proxy decision-making under high uncertainty.Therefore,nurses supporting proxy decision-making should assess the family’s situation and provide cues that facilitate informed and confident decisions.展开更多
Conditional proxy re-encryption(CPRE)is an effective cryptographic primitive language that enhances the access control mechanism and makes the delegation of decryption permissions more granular,but most of the attribu...Conditional proxy re-encryption(CPRE)is an effective cryptographic primitive language that enhances the access control mechanism and makes the delegation of decryption permissions more granular,but most of the attribute-based conditional proxy re-encryption(AB-CPRE)schemes proposed so far do not take into account the importance of user attributes.A weighted attribute-based conditional proxy re-encryption(WAB-CPRE)scheme is thus designed to provide more precise decryption rights delegation.By introducing the concept of weight attributes,the quantity of system attributes managed by the server is reduced greatly.At the same time,a weighted tree structure is constructed to simplify the expression of access structure effectively.With conditional proxy re-encryption,large amounts of data and complex computations are outsourced to cloud servers,so the data owner(DO)can revoke the user’s decryption rights directly with minimal costs.The scheme proposed achieves security against chosen plaintext attacks(CPA).Experimental simulation results demonstrated that the decryption time is within 6–9 ms,and it has a significant reduction in communication and computation cost on the user side with better functionality compared to other related schemes,which enables users to access cloud data on devices with limited resources.展开更多
Salinity is a fundamental variable of watermass chemistry,often varying strongly in coastal,estuarine,lagoonal,restricted-marine basinal,and non-freshwater lacustrine systems(Figure 1).Salinity variation commonly cont...Salinity is a fundamental variable of watermass chemistry,often varying strongly in coastal,estuarine,lagoonal,restricted-marine basinal,and non-freshwater lacustrine systems(Figure 1).Salinity variation commonly controls other watermass properties(e.g.,redox,temperature,p H,and chemical composition)as well as nutrient levels(and thus bioproductivity).展开更多
With the principles of microwave circuits and semiconductor device physics, two microwave power device test circuits combined with a test fixture are designed and simulated, whose properties are evaluated by a paramet...With the principles of microwave circuits and semiconductor device physics, two microwave power device test circuits combined with a test fixture are designed and simulated, whose properties are evaluated by a parameter network analyzer within the frequency range from 3 to 8GHz. The simulation and experimental results verify that the test circuit with a radial stub is better than that without. As an example, a C-band AlGaN/GaN HEMT microwave power device is tested with the designed circuit and fixture. With a 5.4GHz microwave input signal,the maximum gain is 8.75dB,and the maximum output power is 33.2dBm.展开更多
文摘目的旨在通过生物信息学分析结合实验验证,探讨PCBP1(Poly(rC)-Binding Protein 1)在胃癌组织中的表达特征及其临床意义,对其与铁死亡主要调控因子STUB1(STIP1 Homology and U-Box Containing Protein 1)的关系研究。并通过免疫组化实验验证PCBP1与STUB1在胃癌中的表达模式及其与临床病理特征的关系。为新型胃癌靶向治疗策略提供重要的理论支撑和潜在的干预靶点。方法从TIMER 2.0在线分析网站中获取PCBP1在胃癌及癌旁组织的基因表达数据。利用癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库中胃癌数据(STAD)进行KEGG通路富集分析,并揭示其潜在作用机制;在铁死亡调控通路中找出主要调控因子STUB1。随后,采用免疫组化法检测33例胃癌组织及对应癌旁组织中PCBP1和STUB1的表达情况。将所收集的病例依据不同的分化程度、年龄、性别、肿瘤浸润深度、TNM分期及病理学形态进行分组,观察二者的阳性表达率,采用χ^(2)检验分析两者之间及其与临床和病理特征间的相关性,进一步探讨PCBP1和STUB1与胃癌恶性程度的关系。结果免疫组化结果显示PCBP1在癌组织中的阳性表达率为69.7%,明显高于癌旁组织中的阳性表达率48.5%;STUB1在癌组织中的阳性表达率为39.4%,低于癌旁组织中的阳性表达率54.5%,两者差异均有统计学意义(P<0.05)。PCBP1的阳性表达率与肿瘤分化程度、淋巴结转移及Lauren分型有相关性(P<0.05);与患者的年龄、性别、浸润深度、临床分期、神经浸润、脉管侵犯无相关性(P>0.05)。STUB1的阳性表达率与肿瘤分化程度、浸润深度、淋巴结转移及Lauren分型有相关性(P<0.05),但与患者的年龄、性别、临床分期、神经浸润、脉管侵犯无相关性(P>0.05)。PCBP1(癌)与STUB1(癌)的Spearman相关系数为-0.413,P为0.017,表明两者之间存在显著的负相关性。结论PCBP1可通过调控铁死亡通路中主要调控因子STUB1参与胃癌的恶性进展。为胃癌的分子机制探索及潜在治疗靶点提供了新的理论依据。
基金supported by the National Natural Science Foundation of China(Grant No.U24B20146)the National Key Research and Development Plan in China(Grant No.2020YFB1005500)Beijing Natural Science Foundation Project(No.M21034).
文摘With the rapid development of web3.0 applications,the volume of data sharing is increasing,the inefficiency of big data file sharing and the problem of data privacy leakage are becoming more and more prominent,and the existing data sharing schemes have been difficult to meet the growing demand for data sharing,this paper aims at exploring a secure,efficient and privacy-protecting data sharing scheme under web3.0 applications.Specifically,this paper adopts interplanetary file system(IPFS)technology to realize the storage of large data files to solve the problem of blockchain storage capacity limitation,and utilizes ciphertext policy attribute-based encryption(CP-ABE)and proxy re-encryption(PRE)technology to realize secure multi-party sharing and finegrained access control of data.This paper provides the detailed algorithm design and implementation of data sharing phases and processes,and analyzes the algorithms from the perspectives of security,privacy protection,and performance.
文摘Objectives This study aimed to clarify the relationship between the content of proxy decision-making made by families of patients with malignant brain tumors regarding treatment policies and daily care and the cues leading to those decisions.Methods Semi-structured personal interviews were used to collect data.Seven family members of patients with malignant brain tumors were selected to participate in the study by purposive sampling method from June to August 2022 in the Patient Family Association of Japan.Responses were content analyzed to explore the relationship between the content of decisions regarding“treatment policies”and“daily care”and the cues influencing those decisions.Semi-structured interviews were analyzed by using thematic analysis.Results The contents of proxy decisions regarding“treatment policies”included implementation,interruption,and termination of initial treatments,free medical treatments,use of respirators,and end-of-life sedation and included six cues:treatment policies suggested by the primary physician,information and knowledge about the disease and treatment obtained by the family from limited resources,perceived life threat from symptom worsening,words and reactions from the patient regarding treatment,patient’s personality and way of life inferred from their treatment preferences,family’s thoughts and values hoping for better treatment for the patient.Decisions for“daily care”included meal content and methods,excretion,mobility,maintaining cleanliness,rehabilitation,continuation or resignation from work,treatment settings(outpatient or inpatient),and ways to spend time outside and included seven cues:words and thoughts from the patient about their way of life,patient’s reactions and life history inferred from their preferred way of living,things the patient can do to maintain daily life and roles,awareness of the increasing inability to do things in daily life,family’s underlying thoughts and values about how to spend the remaining time,approval from family members regarding the care setting,advice from medical professionals on living at home.Conclusions For“treatment policies,”guidelines from medical professionals were a key cue,while for“daily care,”the small signs from the patients in their daily lives served as cues for proxy decision-making.This may be due to the lack of information available to families and the limited time available for discussion with the patient.Families of patients with malignant brain tumors repeatedly use multiple cues to make proxy decision-making under high uncertainty.Therefore,nurses supporting proxy decision-making should assess the family’s situation and provide cues that facilitate informed and confident decisions.
基金Programs for Science and Technology Development of Henan Province,grant number 242102210152The Fundamental Research Funds for the Universities of Henan Province,grant number NSFRF240620+1 种基金Key Scientific Research Project of Henan Higher Education Institutions,grant number 24A520015Henan Key Laboratory of Network Cryptography Technology,grant number LNCT2022-A11.
文摘Conditional proxy re-encryption(CPRE)is an effective cryptographic primitive language that enhances the access control mechanism and makes the delegation of decryption permissions more granular,but most of the attribute-based conditional proxy re-encryption(AB-CPRE)schemes proposed so far do not take into account the importance of user attributes.A weighted attribute-based conditional proxy re-encryption(WAB-CPRE)scheme is thus designed to provide more precise decryption rights delegation.By introducing the concept of weight attributes,the quantity of system attributes managed by the server is reduced greatly.At the same time,a weighted tree structure is constructed to simplify the expression of access structure effectively.With conditional proxy re-encryption,large amounts of data and complex computations are outsourced to cloud servers,so the data owner(DO)can revoke the user’s decryption rights directly with minimal costs.The scheme proposed achieves security against chosen plaintext attacks(CPA).Experimental simulation results demonstrated that the decryption time is within 6–9 ms,and it has a significant reduction in communication and computation cost on the user side with better functionality compared to other related schemes,which enables users to access cloud data on devices with limited resources.
文摘Salinity is a fundamental variable of watermass chemistry,often varying strongly in coastal,estuarine,lagoonal,restricted-marine basinal,and non-freshwater lacustrine systems(Figure 1).Salinity variation commonly controls other watermass properties(e.g.,redox,temperature,p H,and chemical composition)as well as nutrient levels(and thus bioproductivity).
文摘With the principles of microwave circuits and semiconductor device physics, two microwave power device test circuits combined with a test fixture are designed and simulated, whose properties are evaluated by a parameter network analyzer within the frequency range from 3 to 8GHz. The simulation and experimental results verify that the test circuit with a radial stub is better than that without. As an example, a C-band AlGaN/GaN HEMT microwave power device is tested with the designed circuit and fixture. With a 5.4GHz microwave input signal,the maximum gain is 8.75dB,and the maximum output power is 33.2dBm.
