Several species of mushrooms, as Pleurotus ostreatus, have been valued as edible and medicinal resources. These mushrooms may be an important source of polysaccharides with medicinal properties as antioxidant, antitum...Several species of mushrooms, as Pleurotus ostreatus, have been valued as edible and medicinal resources. These mushrooms may be an important source of polysaccharides with medicinal properties as antioxidant, antitumoral, antimicrobial and immunological properties. The aim of this work was to produce and to evaluate the biological properties of protein-bound polysaccharide complexes, extra intracellular （E-PPS and I-PPS）, extracted from P. ostreatus cultures, using agricultural sunflower wastes as carbon source. Three main compounds in the E-PPS and four main compounds in the I-PPS were identified by SEC-UV-RI-HPLC. These complexes of P. ostreatus present no toxicity in Artemia salina cultures, after 24 h of incubation. Antioxidant properties of the complexes were evaluated by radical scavenging activity using DPPH method and lipid peroxidation inhibition capacity, determined by erytbsocytes hemolysis. Additionally, E-PPS and I-PPS extracts revealed capacity to mimetize superoxide dismutase and catalase enzymatic activities. The hepatoprotector effect of E-PPS extracts in Wistar rats was evaluated by AST, ALT, ALP and y-GT activities, showing capacity to reduce the liver damage induced by ethanol-administration. This hepatoprotective effect is equivalent to that observed by silymarin, a standard drug. Our results suggests that the extracts of E-PPS and I-PPS produced by P. ostreatus cultures, using agricultural sunflower wastes as main carbon source, can be used as an important source of bioactive compounds with potential medicinal value.展开更多
This study was designed to explore the curcumin delivery properties of emulsion gel fabricated by myofibrillar protein(MP)-carboxymethyl cellulose(CMC)complexes with different pHs.Firstly,the MP-CMC complexes under pH...This study was designed to explore the curcumin delivery properties of emulsion gel fabricated by myofibrillar protein(MP)-carboxymethyl cellulose(CMC)complexes with different pHs.Firstly,the MP-CMC complexes under pH 2,3,4,5,and 6 were prepared,then the properties of these complex-stabilized emulsions were tested,and the gel properties and curcumin delivery ability of these heat-induced emulsion gels were compared.Results indicated that MP-CMC complexes showed different viscosities and combined states under different pHs.The emulsions fabricated by pH 6 complexes showed the highest emulsion activity index and emulsion stability,and the lowest droplet size value.The emulsion gel of the pH 6 complex showed the highest hardness,chewiness,gumminess,and resilience,while the pH 4 sample presented the lowest water and oil holding capacity among all samples.The pH 4 samples showed 99%curcumin-encapsulating efficiency as well as 70%curcumin retention after 4 h gastrointestinal digestion.The pH 4 samples could protect and control the release of the curcumin by protecting some proteins from pepsin digestion and controlling the release of fatty acid.As for curcumin release rate and bioavailability at the intestinal stage,the pH 6 samples showed the highest values,followed by the pH 5 samples,and the pH 4 showed the lowest.The emulsion gel prepared by pH 4 MP-CMC complexes could best protect and control the release of curcumin,whilst the pH 6 samples showed a better gel quality and a higher release rate and curcumin bioavailability.展开更多
For more than a decade,the discovery of liquid–liquid phase separation within living organisms has prompted colloid scientists to understand the connection between coacervate functionality,phase behavior,and dynamics...For more than a decade,the discovery of liquid–liquid phase separation within living organisms has prompted colloid scientists to understand the connection between coacervate functionality,phase behavior,and dynamics at a multidisciplinary level.Although the protein–polysaccharide was the first system in which the coacervation phenomenon was discovered and is widely used in food systems,the phase state and relaxation dynamics of protein–polysaccharide complex coacervates(PPCC)have rarely been discussed previously.Consequently,this review aims to unravel the relationship between PPCC dynamics,thermodynamics,molecular architecture,applications,and phase states in past studies.Looking ahead,solving the way molecular architecture spreads to macro-functionality,that is,establishing the relationship between molecular architecture–dynamics–application,will catalyze novel advancements in PPCC research within the field of foods and biomaterials.展开更多
文摘Several species of mushrooms, as Pleurotus ostreatus, have been valued as edible and medicinal resources. These mushrooms may be an important source of polysaccharides with medicinal properties as antioxidant, antitumoral, antimicrobial and immunological properties. The aim of this work was to produce and to evaluate the biological properties of protein-bound polysaccharide complexes, extra intracellular （E-PPS and I-PPS）, extracted from P. ostreatus cultures, using agricultural sunflower wastes as carbon source. Three main compounds in the E-PPS and four main compounds in the I-PPS were identified by SEC-UV-RI-HPLC. These complexes of P. ostreatus present no toxicity in Artemia salina cultures, after 24 h of incubation. Antioxidant properties of the complexes were evaluated by radical scavenging activity using DPPH method and lipid peroxidation inhibition capacity, determined by erytbsocytes hemolysis. Additionally, E-PPS and I-PPS extracts revealed capacity to mimetize superoxide dismutase and catalase enzymatic activities. The hepatoprotector effect of E-PPS extracts in Wistar rats was evaluated by AST, ALT, ALP and y-GT activities, showing capacity to reduce the liver damage induced by ethanol-administration. This hepatoprotective effect is equivalent to that observed by silymarin, a standard drug. Our results suggests that the extracts of E-PPS and I-PPS produced by P. ostreatus cultures, using agricultural sunflower wastes as main carbon source, can be used as an important source of bioactive compounds with potential medicinal value.
基金financially supported by the China Agriculture Research System of MOF and MARA and the Postgraduate Research and Practice Innovation Program of Jiangsu Province(KYCX22-0720).
文摘This study was designed to explore the curcumin delivery properties of emulsion gel fabricated by myofibrillar protein(MP)-carboxymethyl cellulose(CMC)complexes with different pHs.Firstly,the MP-CMC complexes under pH 2,3,4,5,and 6 were prepared,then the properties of these complex-stabilized emulsions were tested,and the gel properties and curcumin delivery ability of these heat-induced emulsion gels were compared.Results indicated that MP-CMC complexes showed different viscosities and combined states under different pHs.The emulsions fabricated by pH 6 complexes showed the highest emulsion activity index and emulsion stability,and the lowest droplet size value.The emulsion gel of the pH 6 complex showed the highest hardness,chewiness,gumminess,and resilience,while the pH 4 sample presented the lowest water and oil holding capacity among all samples.The pH 4 samples showed 99%curcumin-encapsulating efficiency as well as 70%curcumin retention after 4 h gastrointestinal digestion.The pH 4 samples could protect and control the release of the curcumin by protecting some proteins from pepsin digestion and controlling the release of fatty acid.As for curcumin release rate and bioavailability at the intestinal stage,the pH 6 samples showed the highest values,followed by the pH 5 samples,and the pH 4 showed the lowest.The emulsion gel prepared by pH 4 MP-CMC complexes could best protect and control the release of curcumin,whilst the pH 6 samples showed a better gel quality and a higher release rate and curcumin bioavailability.
基金China Postdoctoral Science Foundation,Grant/Award Number:2023M731135Ministry of Science and Technology of the People’s Republic of China,Grant/Award Number:DL2022163005L+2 种基金National Natural Science Foundation of China,Grant/Award Number:32172257Higher Education Discipline Innovation Project,Grant/Award Number:B17018Postdoctoral Innovative Talent Support Program,Grant/Award Number:BX20230130。
文摘For more than a decade,the discovery of liquid–liquid phase separation within living organisms has prompted colloid scientists to understand the connection between coacervate functionality,phase behavior,and dynamics at a multidisciplinary level.Although the protein–polysaccharide was the first system in which the coacervation phenomenon was discovered and is widely used in food systems,the phase state and relaxation dynamics of protein–polysaccharide complex coacervates(PPCC)have rarely been discussed previously.Consequently,this review aims to unravel the relationship between PPCC dynamics,thermodynamics,molecular architecture,applications,and phase states in past studies.Looking ahead,solving the way molecular architecture spreads to macro-functionality,that is,establishing the relationship between molecular architecture–dynamics–application,will catalyze novel advancements in PPCC research within the field of foods and biomaterials.
