Several species of mushrooms, as Pleurotus ostreatus, have been valued as edible and medicinal resources. These mushrooms may be an important source of polysaccharides with medicinal properties as antioxidant, antitum...Several species of mushrooms, as Pleurotus ostreatus, have been valued as edible and medicinal resources. These mushrooms may be an important source of polysaccharides with medicinal properties as antioxidant, antitumoral, antimicrobial and immunological properties. The aim of this work was to produce and to evaluate the biological properties of protein-bound polysaccharide complexes, extra intracellular （E-PPS and I-PPS）, extracted from P. ostreatus cultures, using agricultural sunflower wastes as carbon source. Three main compounds in the E-PPS and four main compounds in the I-PPS were identified by SEC-UV-RI-HPLC. These complexes of P. ostreatus present no toxicity in Artemia salina cultures, after 24 h of incubation. Antioxidant properties of the complexes were evaluated by radical scavenging activity using DPPH method and lipid peroxidation inhibition capacity, determined by erytbsocytes hemolysis. Additionally, E-PPS and I-PPS extracts revealed capacity to mimetize superoxide dismutase and catalase enzymatic activities. The hepatoprotector effect of E-PPS extracts in Wistar rats was evaluated by AST, ALT, ALP and y-GT activities, showing capacity to reduce the liver damage induced by ethanol-administration. This hepatoprotective effect is equivalent to that observed by silymarin, a standard drug. Our results suggests that the extracts of E-PPS and I-PPS produced by P. ostreatus cultures, using agricultural sunflower wastes as main carbon source, can be used as an important source of bioactive compounds with potential medicinal value.展开更多
In this study,the in vivo antioxidant and anti-inflammatory effect of fucoxanthin-loaded fucoidan-ovalbumin(Fx-FO)nanoparticles on acetic acid-induced ulcerative colitis(UC)rat model was investigated.First,UC was indu...In this study,the in vivo antioxidant and anti-inflammatory effect of fucoxanthin-loaded fucoidan-ovalbumin(Fx-FO)nanoparticles on acetic acid-induced ulcerative colitis(UC)rat model was investigated.First,UC was induced by rectal administration of 1 ml of 3%acetic acid,then rats were treated daily with Fx-FO nanoparticles(50 and 100 mg/kg),fucoxanthin(Fx:100 mg/kg),fucoidan(Fd:25 mg/kg),mesalazine(Mes:10 mg/kg),and asacol(As:10 mg/kg).via gavage and enema for 7 days.Treatments were done by 1 mL gavage and enema daily for 7 days,and then weight,colonic shortening,antioxidant(scavenging DPPH,MDA,MPO,and SOD)and inflammation(TNF-α,IL-6 and IL-1β)activities,and histopathological effects were investigated.According to the results,all UC rats exhibited weight loss and shortening of colon tissue to different extents.While these were considerably suppressed in the Fx-FO100 nanoparticles in induced-UC rats.Moreover,Fx-FO100 treated-group inhibited the inflammatory response via reducing the content of inflammatory factors(TNF-α,IL-1βand IL-6).Fx-FO100 caused a decrease in the MPO,MDA levels and increased the SOD activity in the colons of rats with UC.Also,rectal treatment improvement was more frequent than treatment with oral administration.Moreover,histology of the colon showed the destruction of the intestinal barrier with complete loss of the epithelial layer and severe transmural inflammatory cell integrity in the UC group,while administration of Fx-FO nanoparticles exhibited marked improvement in mucosal repair with attenuation of inflammation.This study provides a new protective nutrient to develop functional foods with the potential of UC symptom modulation and keep intestinal function.展开更多
This study was designed to explore the curcumin delivery properties of emulsion gel fabricated by myofibrillar protein(MP)-carboxymethyl cellulose(CMC)complexes with different pHs.Firstly,the MP-CMC complexes under pH...This study was designed to explore the curcumin delivery properties of emulsion gel fabricated by myofibrillar protein(MP)-carboxymethyl cellulose(CMC)complexes with different pHs.Firstly,the MP-CMC complexes under pH 2,3,4,5,and 6 were prepared,then the properties of these complex-stabilized emulsions were tested,and the gel properties and curcumin delivery ability of these heat-induced emulsion gels were compared.Results indicated that MP-CMC complexes showed different viscosities and combined states under different pHs.The emulsions fabricated by pH 6 complexes showed the highest emulsion activity index and emulsion stability,and the lowest droplet size value.The emulsion gel of the pH 6 complex showed the highest hardness,chewiness,gumminess,and resilience,while the pH 4 sample presented the lowest water and oil holding capacity among all samples.The pH 4 samples showed 99%curcumin-encapsulating efficiency as well as 70%curcumin retention after 4 h gastrointestinal digestion.The pH 4 samples could protect and control the release of the curcumin by protecting some proteins from pepsin digestion and controlling the release of fatty acid.As for curcumin release rate and bioavailability at the intestinal stage,the pH 6 samples showed the highest values,followed by the pH 5 samples,and the pH 4 showed the lowest.The emulsion gel prepared by pH 4 MP-CMC complexes could best protect and control the release of curcumin,whilst the pH 6 samples showed a better gel quality and a higher release rate and curcumin bioavailability.展开更多
For more than a decade,the discovery of liquid–liquid phase separation within living organisms has prompted colloid scientists to understand the connection between coacervate functionality,phase behavior,and dynamics...For more than a decade,the discovery of liquid–liquid phase separation within living organisms has prompted colloid scientists to understand the connection between coacervate functionality,phase behavior,and dynamics at a multidisciplinary level.Although the protein–polysaccharide was the first system in which the coacervation phenomenon was discovered and is widely used in food systems,the phase state and relaxation dynamics of protein–polysaccharide complex coacervates(PPCC)have rarely been discussed previously.Consequently,this review aims to unravel the relationship between PPCC dynamics,thermodynamics,molecular architecture,applications,and phase states in past studies.Looking ahead,solving the way molecular architecture spreads to macro-functionality,that is,establishing the relationship between molecular architecture–dynamics–application,will catalyze novel advancements in PPCC research within the field of foods and biomaterials.展开更多
Although the health benefits of lipophilic bioactive compounds have received tremendous attention from both researchers and manufacturers,the practical application of these micronutrients in aqueous foods is still cha...Although the health benefits of lipophilic bioactive compounds have received tremendous attention from both researchers and manufacturers,the practical application of these micronutrients in aqueous foods is still chal-lenging due to their water insolubility and environmental instability.Therefore,curcumin(Cur)was selected to be encapsulated in soybean protein isolate-dextran conjugate-based nanogels(SDCNG)via the Maillard reaction combined with protein self-assembly to be incorporated into orange juice beverage(OJB)in this study.Curloaded SDCNG(Cur-SDCNG)prepared by adding Cur into the SDCNG solution exhibited an encapsulation effi-ciency of 89.10%±1.40%and a loading capacity of 17.11%±0.27%,enhanced 1,1-Diphenyl-2-picrylhydrazyl(DPPH)radical and hydroxyl radical(·OH)-scavenging activities and ferrous reducing power,increased reten-tion rate at both natural light and dark environment,and strengthened storage stability.Moreover,the loaded Cur showed a greater bioaccessibility(55.41%±0.71%)than free Cur(17.41%±1.04%).The OJB fortified with Cur by adding Cur-SDCNG exhibited an increasing color difference,particle size,polydispersity index(PDI),protein content,total soluble solid(TSS),and sensory score with the increasing Cur concentration.DPPH radical and·OH-scavenging activities and ferrous reducing power also further enhanced.The Cur-fortified OJB with an orange juice concentration of 30%exhibited improved quality attributes and satisfactory stability reflected by the increased TSS and sensory score,acceptable particle size,PDI,centrifugal sedimentation rate,and Cur retention rate under cold conditions.In conclusion,the findings confirmed the effectiveness of Cur encapsulation in SDCNG and the application feasibility of Cur-SDCNG in acidic beverages simultaneously fortified with lipo-philic bioactive compounds and vegetable proteins.展开更多
Porphyra haitanensis with many bioactive components has great prospects for application in the functional food,pharmaceutical,and cosmeceutical industries.In this study,two sequentially extracted fractions(PhE1&Ph...Porphyra haitanensis with many bioactive components has great prospects for application in the functional food,pharmaceutical,and cosmeceutical industries.In this study,two sequentially extracted fractions(PhE1&PhE2)were isolated and structurally characterized.The results showed that PhE1 was mainly composed of protein(36.82%)and neutral sugar(32.43%),while PhE2 mainly contained neutral sugar(58.70%).Both had galactose as main monosaccharides,PhE2 also contained a small amount of glucose.To widen the application of PhEs in starchy foods,they were added to corn starch(CS)system and co-gelatinized.Rheological tests indicated that PhEs could effectively affect gel strength of CS.The addition of PhEs effectively inhibited the content of leached amylose in CS gelatinization system except those at an addition of 5%PhE1 and 5%PhE2.FT-IR data suggested that PhE1 could improve the short-range order,but PhE2 showed no significant effects.SEM images showed that the pore wall of the composites thickened,and the network structure became relatively ordered.In vitro tests,PhEs significantly reduced the contents of rapidly digestible starch,and PhE1 presented better effects.Therefore,PhEs may be used as potential materials to delay the digestion of CS and related products.展开更多
文摘Several species of mushrooms, as Pleurotus ostreatus, have been valued as edible and medicinal resources. These mushrooms may be an important source of polysaccharides with medicinal properties as antioxidant, antitumoral, antimicrobial and immunological properties. The aim of this work was to produce and to evaluate the biological properties of protein-bound polysaccharide complexes, extra intracellular （E-PPS and I-PPS）, extracted from P. ostreatus cultures, using agricultural sunflower wastes as carbon source. Three main compounds in the E-PPS and four main compounds in the I-PPS were identified by SEC-UV-RI-HPLC. These complexes of P. ostreatus present no toxicity in Artemia salina cultures, after 24 h of incubation. Antioxidant properties of the complexes were evaluated by radical scavenging activity using DPPH method and lipid peroxidation inhibition capacity, determined by erytbsocytes hemolysis. Additionally, E-PPS and I-PPS extracts revealed capacity to mimetize superoxide dismutase and catalase enzymatic activities. The hepatoprotector effect of E-PPS extracts in Wistar rats was evaluated by AST, ALT, ALP and y-GT activities, showing capacity to reduce the liver damage induced by ethanol-administration. This hepatoprotective effect is equivalent to that observed by silymarin, a standard drug. Our results suggests that the extracts of E-PPS and I-PPS produced by P. ostreatus cultures, using agricultural sunflower wastes as main carbon source, can be used as an important source of bioactive compounds with potential medicinal value.
基金funded by Iran National Science Foundation(INSF)under project No,4031631.
文摘In this study,the in vivo antioxidant and anti-inflammatory effect of fucoxanthin-loaded fucoidan-ovalbumin(Fx-FO)nanoparticles on acetic acid-induced ulcerative colitis(UC)rat model was investigated.First,UC was induced by rectal administration of 1 ml of 3%acetic acid,then rats were treated daily with Fx-FO nanoparticles(50 and 100 mg/kg),fucoxanthin(Fx:100 mg/kg),fucoidan(Fd:25 mg/kg),mesalazine(Mes:10 mg/kg),and asacol(As:10 mg/kg).via gavage and enema for 7 days.Treatments were done by 1 mL gavage and enema daily for 7 days,and then weight,colonic shortening,antioxidant(scavenging DPPH,MDA,MPO,and SOD)and inflammation(TNF-α,IL-6 and IL-1β)activities,and histopathological effects were investigated.According to the results,all UC rats exhibited weight loss and shortening of colon tissue to different extents.While these were considerably suppressed in the Fx-FO100 nanoparticles in induced-UC rats.Moreover,Fx-FO100 treated-group inhibited the inflammatory response via reducing the content of inflammatory factors(TNF-α,IL-1βand IL-6).Fx-FO100 caused a decrease in the MPO,MDA levels and increased the SOD activity in the colons of rats with UC.Also,rectal treatment improvement was more frequent than treatment with oral administration.Moreover,histology of the colon showed the destruction of the intestinal barrier with complete loss of the epithelial layer and severe transmural inflammatory cell integrity in the UC group,while administration of Fx-FO nanoparticles exhibited marked improvement in mucosal repair with attenuation of inflammation.This study provides a new protective nutrient to develop functional foods with the potential of UC symptom modulation and keep intestinal function.
基金financially supported by the China Agriculture Research System of MOF and MARA and the Postgraduate Research and Practice Innovation Program of Jiangsu Province(KYCX22-0720).
文摘This study was designed to explore the curcumin delivery properties of emulsion gel fabricated by myofibrillar protein(MP)-carboxymethyl cellulose(CMC)complexes with different pHs.Firstly,the MP-CMC complexes under pH 2,3,4,5,and 6 were prepared,then the properties of these complex-stabilized emulsions were tested,and the gel properties and curcumin delivery ability of these heat-induced emulsion gels were compared.Results indicated that MP-CMC complexes showed different viscosities and combined states under different pHs.The emulsions fabricated by pH 6 complexes showed the highest emulsion activity index and emulsion stability,and the lowest droplet size value.The emulsion gel of the pH 6 complex showed the highest hardness,chewiness,gumminess,and resilience,while the pH 4 sample presented the lowest water and oil holding capacity among all samples.The pH 4 samples showed 99%curcumin-encapsulating efficiency as well as 70%curcumin retention after 4 h gastrointestinal digestion.The pH 4 samples could protect and control the release of the curcumin by protecting some proteins from pepsin digestion and controlling the release of fatty acid.As for curcumin release rate and bioavailability at the intestinal stage,the pH 6 samples showed the highest values,followed by the pH 5 samples,and the pH 4 showed the lowest.The emulsion gel prepared by pH 4 MP-CMC complexes could best protect and control the release of curcumin,whilst the pH 6 samples showed a better gel quality and a higher release rate and curcumin bioavailability.
基金China Postdoctoral Science Foundation,Grant/Award Number:2023M731135Ministry of Science and Technology of the People’s Republic of China,Grant/Award Number:DL2022163005L+2 种基金National Natural Science Foundation of China,Grant/Award Number:32172257Higher Education Discipline Innovation Project,Grant/Award Number:B17018Postdoctoral Innovative Talent Support Program,Grant/Award Number:BX20230130。
文摘For more than a decade,the discovery of liquid–liquid phase separation within living organisms has prompted colloid scientists to understand the connection between coacervate functionality,phase behavior,and dynamics at a multidisciplinary level.Although the protein–polysaccharide was the first system in which the coacervation phenomenon was discovered and is widely used in food systems,the phase state and relaxation dynamics of protein–polysaccharide complex coacervates(PPCC)have rarely been discussed previously.Consequently,this review aims to unravel the relationship between PPCC dynamics,thermodynamics,molecular architecture,applications,and phase states in past studies.Looking ahead,solving the way molecular architecture spreads to macro-functionality,that is,establishing the relationship between molecular architecture–dynamics–application,will catalyze novel advancements in PPCC research within the field of foods and biomaterials.
基金financially supported by the National Natural Science Foundation of China(Grant No.32101981)the Natural Science Foun-dation of Sichuan Province(2022NSFSC1684)the cooperation project between Ya’an city and Sichuan Agricultural University(23ZDF0003).
文摘Although the health benefits of lipophilic bioactive compounds have received tremendous attention from both researchers and manufacturers,the practical application of these micronutrients in aqueous foods is still chal-lenging due to their water insolubility and environmental instability.Therefore,curcumin(Cur)was selected to be encapsulated in soybean protein isolate-dextran conjugate-based nanogels(SDCNG)via the Maillard reaction combined with protein self-assembly to be incorporated into orange juice beverage(OJB)in this study.Curloaded SDCNG(Cur-SDCNG)prepared by adding Cur into the SDCNG solution exhibited an encapsulation effi-ciency of 89.10%±1.40%and a loading capacity of 17.11%±0.27%,enhanced 1,1-Diphenyl-2-picrylhydrazyl(DPPH)radical and hydroxyl radical(·OH)-scavenging activities and ferrous reducing power,increased reten-tion rate at both natural light and dark environment,and strengthened storage stability.Moreover,the loaded Cur showed a greater bioaccessibility(55.41%±0.71%)than free Cur(17.41%±1.04%).The OJB fortified with Cur by adding Cur-SDCNG exhibited an increasing color difference,particle size,polydispersity index(PDI),protein content,total soluble solid(TSS),and sensory score with the increasing Cur concentration.DPPH radical and·OH-scavenging activities and ferrous reducing power also further enhanced.The Cur-fortified OJB with an orange juice concentration of 30%exhibited improved quality attributes and satisfactory stability reflected by the increased TSS and sensory score,acceptable particle size,PDI,centrifugal sedimentation rate,and Cur retention rate under cold conditions.In conclusion,the findings confirmed the effectiveness of Cur encapsulation in SDCNG and the application feasibility of Cur-SDCNG in acidic beverages simultaneously fortified with lipo-philic bioactive compounds and vegetable proteins.
基金supported by National Natural Science Foundation of China(No.32202019)Natural Science Foundation of Fujian Province(No.2022J05023)Research Project of Jiangxi Normal University(NO.12022571).
文摘Porphyra haitanensis with many bioactive components has great prospects for application in the functional food,pharmaceutical,and cosmeceutical industries.In this study,two sequentially extracted fractions(PhE1&PhE2)were isolated and structurally characterized.The results showed that PhE1 was mainly composed of protein(36.82%)and neutral sugar(32.43%),while PhE2 mainly contained neutral sugar(58.70%).Both had galactose as main monosaccharides,PhE2 also contained a small amount of glucose.To widen the application of PhEs in starchy foods,they were added to corn starch(CS)system and co-gelatinized.Rheological tests indicated that PhEs could effectively affect gel strength of CS.The addition of PhEs effectively inhibited the content of leached amylose in CS gelatinization system except those at an addition of 5%PhE1 and 5%PhE2.FT-IR data suggested that PhE1 could improve the short-range order,but PhE2 showed no significant effects.SEM images showed that the pore wall of the composites thickened,and the network structure became relatively ordered.In vitro tests,PhEs significantly reduced the contents of rapidly digestible starch,and PhE1 presented better effects.Therefore,PhEs may be used as potential materials to delay the digestion of CS and related products.
