The mutagenicity of the protein-modified Enterococcus faecalis was evaluated by a mouse bone marrow micronucleus test and a mouse sperm abnormality test.The test substance was designed with three dose groups (1,2 and ...The mutagenicity of the protein-modified Enterococcus faecalis was evaluated by a mouse bone marrow micronucleus test and a mouse sperm abnormality test.The test substance was designed with three dose groups (1,2 and 5 g/kg·bw) and intragastrically administrated,with cyclophosphamide as a positive control and normal saline as a normal control.The micronucleus rate and sperm abnormality rate were measured.The results showed that the micronucleus rates and sperm abnormality rates in the different dose groups were not significantly different from those in normal control group ( P >0.05),while the positive control group was significantly higher than the normal control group ( P <0.01).The conclusion is that the protein-modified E.faecalis was tested to be negative in both the mouse bone marrow micronucleus test and mouse sperm abnormality test,suggesting that it has no mutagenicity in vivo.展开更多
The potential mutagenicity of protein-modified Enterococcus faecalis was evaluated by the Ames test. The test subject was designed with 0. 05,0. 5,5. 0,and 50. 0 mg/dish doses,and a control group was set. With Salmone...The potential mutagenicity of protein-modified Enterococcus faecalis was evaluated by the Ames test. The test subject was designed with 0. 05,0. 5,5. 0,and 50. 0 mg/dish doses,and a control group was set. With Salmonella typhimurium mutant strains TA97,TA98,TA100 and TA102 as test strains,the Ames test was carried out with( +) or without(-) S9,and the number of revertant colonies was counted. The results showed that for the protein-modified E. faecalis in the case of + S9 and-S9,the average numbers of revertant colonies of the four test strains were less than two times of that of the negative control group,and no dose-response relationship was observed. The protein-modified E. faecalis was tested to be negative in the Ames test,indicating that the test substance was not mutagenic in vitro.展开更多
基金Supported by Top-notch Academic Programs Project of Jiangsu Higher Education Institutions(PPZY2015C230)
文摘The mutagenicity of the protein-modified Enterococcus faecalis was evaluated by a mouse bone marrow micronucleus test and a mouse sperm abnormality test.The test substance was designed with three dose groups (1,2 and 5 g/kg·bw) and intragastrically administrated,with cyclophosphamide as a positive control and normal saline as a normal control.The micronucleus rate and sperm abnormality rate were measured.The results showed that the micronucleus rates and sperm abnormality rates in the different dose groups were not significantly different from those in normal control group ( P >0.05),while the positive control group was significantly higher than the normal control group ( P <0.01).The conclusion is that the protein-modified E.faecalis was tested to be negative in both the mouse bone marrow micronucleus test and mouse sperm abnormality test,suggesting that it has no mutagenicity in vivo.
基金Supported by Top-notch Academic Programs Project of Jiangsu Higher Education Institutions(PPZY2015C230)
文摘The potential mutagenicity of protein-modified Enterococcus faecalis was evaluated by the Ames test. The test subject was designed with 0. 05,0. 5,5. 0,and 50. 0 mg/dish doses,and a control group was set. With Salmonella typhimurium mutant strains TA97,TA98,TA100 and TA102 as test strains,the Ames test was carried out with( +) or without(-) S9,and the number of revertant colonies was counted. The results showed that for the protein-modified E. faecalis in the case of + S9 and-S9,the average numbers of revertant colonies of the four test strains were less than two times of that of the negative control group,and no dose-response relationship was observed. The protein-modified E. faecalis was tested to be negative in the Ames test,indicating that the test substance was not mutagenic in vitro.
