Elastic behaviors of protein-like chains are investigated by Pruned-Enriched-Rosenbluth method and modified orientation-dependent monomer-monomer interactions model. The protein-like chain is pulled away from the attr...Elastic behaviors of protein-like chains are investigated by Pruned-Enriched-Rosenbluth method and modified orientation-dependent monomer-monomer interactions model. The protein-like chain is pulled away from the attractive surface slowly with elastic force acting on it. Strong adsorption interaction and no adsorption interaction are both considered. We calculate the characteristic ratio and shape factor of protein-like chains in the process of elongation. The conformation change of the protein-like chain is well depicted. The shape of chain changes from “rod” to “sphere” at the beginning of elongation. Then, the shape changes from “sphere” to “rod”. In the end, the shape becomes a “sphere” as the chain leaves away from the surface. In the meantime, we discuss average Helmoholtz free energy per bond, average energy per bond, average adsorbed energy per bond, average α-helical energy per bond, average β-sheet energy per bond and average contact energy per bond. On the other hand, elastic force is also studied. It is found that elastic force has a long plateau during the tensile elongation when there exists adsorption interaction. This result is consistent with SMFS experiment of general polymers. Energy contribution to elastic force and contact energy contribution to elastic force are both discussed. These investigations can provide some insights into the elastic behaviors of adsorbed protein chains.展开更多
The effect of channel-protein interaction on the translocation of a protein-like chain through a finite channel under certain electric field was studied by using dynamical Monte Carlo simulations. The interior behavio...The effect of channel-protein interaction on the translocation of a protein-like chain through a finite channel under certain electric field was studied by using dynamical Monte Carlo simulations. The interior behavior of chain conformation under different interactions was investigated, such as the number of monomers outside of channel nout, monomers inside of channel nm, mean-square radius of gyration 〈 S2 〉 and the average energy 〈U〉. It shows that with strong attractive interaction, the translocation is more difficult than moderate interaction. At the same time, the dependence of translocation time with different interactions shows that moderate repulsive interaction （εcp = 0.5） accelerates the translocation. Although the waiting time for successful translocation of εep = 1.0 is the longest, the average translocation time is not very large. It is far smaller than that of εep = -1.0. The probability distributions of translocation time p（t＇） and the probability distributions of three duration times p（t1＇）, p（t2＇） and p（t3＇） were all discussed. Log-normal distributions are found. All these findings will strengthen the understanding of protein translocation.展开更多
MicroRNAs(miRNAs)are endogenous small non-coding RNAs that play an important role in post-transcriptional gene regulation in plants and animals by targeting messenger RNAs(mRNAs)for cleavage or repressing translation ...MicroRNAs(miRNAs)are endogenous small non-coding RNAs that play an important role in post-transcriptional gene regulation in plants and animals by targeting messenger RNAs(mRNAs)for cleavage or repressing translation of specific mRNAs.The first miRNA identified in plants,miRNA156(miR156),targets the SQUAMOSA promoter-binding protein-like(SPL)transcription factors,which play critical roles in plant phase transition,flower and plant architecture,and fruit development.We identified multiple copies of MIR156 and SPL in the rice,Brachypodium,sorghum,maize,and foxtail millet genomes.Sequence and chromosomal synteny analysis showed that both MIR156s and SPLs are conserved across species in the grass family.Analysis of expression data of the SPLs in eleven juvenile and adult rice tissues revealed that four non-miR156-targeted genes were highly expressed and three miR156-targeted genes were only slightly expressed in all tissues/developmental stages.The remaining SPLs were highly expressed in the juvenile stage,but their expression was lower in the adult stage.It has been proposed that under strong selective pressure,non-miR156-targeted mRNA may be able to re-structure to form a miRNAresponsive element.In our analysis,some non-miR156-targeted SPLs(SPL5/8/10)had gene structure and gene expression patterns similar to those of miR156-targeted genes,suggesting that they could diversify into miR156-targeted genes.DNA methylation profiles of SPLs and MIR156s in different rice tissues showed diverse methylation patterns,and hypomethylation of non-CG sites was observed in rice endosperm.Our findings suggested that MIR156s and SPLs had different origination and evolutionary mechanisms:the SPLs appear to have resulted from vertical evolution,whereas MIR156s appear to have resulted from strong evolutionary selection on mature sequences.展开更多
BACKGROUND Rearrangements of the anaplastic lymphoma kinase(ALK)gene(ALK-positive)represent an oncogenic driver in approximately 3%-5%of non-small-lung cancer(NSCLC)patients.Sarcoidosis is a multisystem disease,and it...BACKGROUND Rearrangements of the anaplastic lymphoma kinase(ALK)gene(ALK-positive)represent an oncogenic driver in approximately 3%-5%of non-small-lung cancer(NSCLC)patients.Sarcoidosis is a multisystem disease,and its reported incidence in Asia is 1 or less per 100000 people per year.The co-occurrence of sarcoidosis and ALK-positive NSCLC is rare,and ALK-positive lung cancer is likely to spread quickly.Therefore,the co-occurrence of sarcoidosis is more easily misdiagnosed as metastatic lung cancer by radiological examination.CASE SUMMARY A 50-year-old man had a nodule in the left superior lobe,many small nodules in left superior and right lungs,and enlarged bilateral hilar,mediastinal,and right supraclavicular lymph nodes.Computed tomography-guided pulmonary biopsy of the nodule in the left superior lobe revealed echinoderm microtubuleassociated protein-like 4 gene-ALK positive NSCLC with concomitant noncaseating granuloma.This patient was treated with crizotinib.Thirty days later,a chest computed tomography scan revealed a dramatic decrease in the size of the left superior lobe nodule;however,the lesions in the right lung progressed.The right supraclavicular lymph nodes showed granulomas,and no tumor cells were identified in the specimens. The angiotensin-converting enzyme level was high.After 1 wk of methylprednisolone treatment, a significant response of all lesionswas revealed. Following radical resection of the lung cancer, noncaseatinggranulomas were observed in both lung tissues and lymph nodes, which resultedin a diagnosis of echinoderm microtubule-associated protein-like 4-ALK positiveNSCLC accompanied with sarcoidosis.CONCLUSIONOur experience illustrates that pathological evidence is needed to confirmmetastatic disease, especially when some suspected metastatic lesions arenegative for malignancy.展开更多
BACKGROUND It is not uncommon to develop viral encephalitis.Epidemic Japanese B encephalitis infection combined with contactin-associated protein-like 2(CASPR-2)antibody-positive autoimmune encephalitis has not been r...BACKGROUND It is not uncommon to develop viral encephalitis.Epidemic Japanese B encephalitis infection combined with contactin-associated protein-like 2(CASPR-2)antibody-positive autoimmune encephalitis has not been reported at present.In clinical work,we need to consider more options.CASE SUMMARY A 32-year-old male worker presented with headache,fever and call-unresponsive presentation.Complete cranial magnetic resonance image showed symmetrical abnormal signals in bilateral medial temporal lobe,bilateral thalamus and basal ganglia.Improved lumbar puncture showed that cerebrospinal fluid protein and cell count increased significantly.Viral encephalitis was considered,and the patient's consciousness still increased rapidly after antiviral treatment.Further detection of Cerebrospinal fluid Japanese B encephalitis virus Polymerase Chain Reaction positive,serum autoimmune encephalitis antibody showed CASPR-2 antibody positive(1:320),the patient's condition gradually improved after plasma exchange treatment.3 mo later,the serum CASPR-2 antibody was negative and the patient's condition was stable.CONCLUSION This article reports the world’s first case of Epidemic Japanese B encephalitis infection combined with CASPR-2 antibody-positive autoimmune encephalitis,with a view to raising awareness.展开更多
Objective： To clone and construct the recombinant plasmid containing ATP synthase lipid-binding protein-like protein gene of Schistosomajaponicum,（SjAslp） and transfer it into mammalian cells to express the objecti...Objective： To clone and construct the recombinant plasmid containing ATP synthase lipid-binding protein-like protein gene of Schistosomajaponicum,（SjAslp） and transfer it into mammalian cells to express the objective protein. Methods： By polymerase chain reaction （PCR） technique, SjAslp was amplified from the constructed recombinant plasmid pBCSK＋/SjAslp, and inserted into cloning vector pUCm-T. Then, SjAslp was subcloned into an eukaryotic expression vector pcDNA3.1（＋）. After identifying it by PCR, restrictive enzymes digestion and DNA sequencing, the recombinant plasmid was transfected into HeLa cells using electroporation, and the expression of the recombinant protein was analyzed by immunocytochemical assay. Results： The specific gene fragment of 558 bp was successfully amplified. The DNA vaccine of SjAslp was successfully constructed. Immunocytochemical assay showed that SjAslp was expressed in the cytoplasm of HeLa cells. Conclusion： SjAslp gene can be expressed in eukaryotic system, which lays the foundation for development of the SjAslp DNA vaccine against schitosomiasis.展开更多
We study the translocation of a protein-like chain through a finite cylindrical channel using the pruned-enriched Rosenbluth method （PERM） and the modified orientation-dependent monomer-monomer interaction （ODI） m...We study the translocation of a protein-like chain through a finite cylindrical channel using the pruned-enriched Rosenbluth method （PERM） and the modified orientation-dependent monomer-monomer interaction （ODI） model. Attractive channels （εcp = -2.0, -1.0, -0.5）, repulsive chanaels （εcp： 0.5, 1.0, 2.0）, and a neutral channel （εcp =- 0） are discussed. The results of the chain dimension and the energy show that Z0 ： 1.0 is an important case to distinguish the types of the channels. For the strong attractive channel, more contacts form during the process of translocation. It is also found that an external force is needed to drive the chain outside of the channel with the strong attraction. While for the neutral, the repulsive, and the weak attractive channels, the translocation is spontaneous.展开更多
The conformational properties and elastic behaviors of protein-like single chains in the process of tensileelongation were investigated by means of Monte Carlo method.The sequences of protein-like single chains contai...The conformational properties and elastic behaviors of protein-like single chains in the process of tensileelongation were investigated by means of Monte Carlo method.The sequences of protein-like single chains contain two typesof residues:hydrophobic(H)and hydrophilic(P).The average conformations and thermodynamics statistical properties ofprotein-like single chains with various elongation ratio λ were calculated.It was found that the mean-square end-to-enddistance<R^2>(?).increases with elongation ratio λ.The tensor eigenvalues ratio of<L_2~2>:<L_1~2>decreases with elongationratio λ for short(HP)_x protein-like polymers,however,the ratio of<L_3~2>:<L_1~2>increases with elongation ratio λ,especially for long (H)_x sequence.Average energy per bond increases with elongation ratio λ,especially for (H)_xprotein-like single chains.Helmholtz free energy per bond also increases with elongation ratio λ.Elastic force(f),energycontribution to force(f_U)and entropy contribution to force(fs)for different protein-like single chains were also calculated.These investigations may provide some insights into elastic behaviors of proteins.展开更多
目的:探究急性大血管闭塞性脑梗死(acute ischemic stroke due to large vessel occlusion,AIS-LVO)机械取栓的预后影响因素,构建列线图模型。方法:选取2020年1月至2023年8月保定市第一中心医院166例AIS-LVO患者,均接受机械取栓,根据取...目的:探究急性大血管闭塞性脑梗死(acute ischemic stroke due to large vessel occlusion,AIS-LVO)机械取栓的预后影响因素,构建列线图模型。方法:选取2020年1月至2023年8月保定市第一中心医院166例AIS-LVO患者,均接受机械取栓,根据取栓后3个月改良Rankin量表评分(modified rankin scale,mRS)分组,比较两组一般资料及血清视锥蛋白样蛋白-1(visininlike protein-1,VILIP-1)、硫氧还蛋白1(thioredoxin-1,Trx1)水平,采用Logistic回归方程、极端梯度提升(extreme gradient boosting,XGBoost)算法对AIS-LVO患者预后的相关因素进行筛选,构建列线图模型,采用受试者工作特征曲线(ROC)及曲线下面积(AUC)分析列线图模型的区分度,采用Bootstrap自抽样法,对数据集进行1000次重抽样的自举验证,进行内部验证并绘制校准曲线,采用决策曲线分析列线图模型的临床有效性。结果:预后不良组血清VILIP-1、入院时美国国立卫生研究院卒中量表(the national institutes of health stroke scale,NIHSS)评分高于预后良好组,Trx1、入院时Alberta卒中项目早期CT(alberta stroke project early CT,ASPECT)评分及CTA评分≥2级比例低于预后良好组(P<0.05);血清VILIP-1、Trx1、CTA评分≥2级及入院时NIHSS评分、ASPECT评分均是AIS-LVO机械取栓预后不良的影响因素(P<0.05);XGBoost算法显示,入院时NIHSS评分的SHAP平均值最高,其次为入院时ASPECT评分、VILIP-1、Trx1及CTA评分≥2级;采用Bootstrap自抽样法对列线图模型进行内部验证,列线图模型的AUCROC为0.927;校准曲线显示,模型的校准曲线观测值和预测值的重合度较好,经Hosmer-Lemeshow优度检验证实预测偏差不存在统计学意义(χ^(2)=5.411,P=0.736);决策曲线显示,列线图模型在0.0~1.0具有明显的正向净收益。结论:联合应用入院时NIHSS评分、ASPECT评分、CTA评分≥2级及血清VILIP-1、Trx1预测AIS-LVO机械取栓预后效能更佳,可帮助临床学者及早识别高危人群,减少预后不良发生。展开更多
目的:分析二肽基肽酶样蛋白-6(dipeptidyl-peptidase-like protein 6,DPPX)抗体相关脑炎患者的临床特征。方法:对2016年1月—2025年2月南京医科大学附属脑科医院就诊的5例DPPX抗体相关脑炎患者的临床特点、脑电图、磁共振成像(magnetic ...目的:分析二肽基肽酶样蛋白-6(dipeptidyl-peptidase-like protein 6,DPPX)抗体相关脑炎患者的临床特征。方法:对2016年1月—2025年2月南京医科大学附属脑科医院就诊的5例DPPX抗体相关脑炎患者的临床特点、脑电图、磁共振成像(magnetic resonance imaging,MRI)及预后进行回顾性研究。结果:5例均为男性,年龄14~56岁。5例患者血清DPPX抗体均为阳性,滴度1∶100~1∶10,其中1例合并血清接触蛋白关联蛋白2(contactin-associated protein-like 2,CASPR2)抗体阳性,4例脑脊液DPPX抗体阴性,1例滴度1∶1。2例患者以行为异常起病,1例以癫痫发作起病,1例以记忆力减退起病,1例合并CASPR2抗体阳性患者以多部位游走性肌阵挛起病。4例患者头颅MRI正常,1例头颅MRI提示双侧颞叶异常信号。以癫痫发作起病的患者脑电图背景重度异常合并左前颞尖波、尖慢波频发。结论:DPPX抗体相关脑炎临床表现具有异质性,早期诊断与鉴别困难,免疫治疗有效,易复发。展开更多
目的探究纤维蛋白原样蛋白1(fibrinogen-like protein 1,FGL1)和膜联蛋白A11(annexin A11,ANXA11)对肺癌手术患者效用评估价值及与预后的相关性。方法将本院2020年1月至2023年12月接诊的98例肺癌患者纳入肺癌组,另选取98例健康人作为对...目的探究纤维蛋白原样蛋白1(fibrinogen-like protein 1,FGL1)和膜联蛋白A11(annexin A11,ANXA11)对肺癌手术患者效用评估价值及与预后的相关性。方法将本院2020年1月至2023年12月接诊的98例肺癌患者纳入肺癌组,另选取98例健康人作为对照组。受试者入院后分别于空腹状态下采取6 mL肘静脉血,采用ELISA法检测血清ANXA11水平,采用实时荧光定量PCR技术检测ANXA11表达水平。根据术后1个月肿瘤标志物变化、影像学表现和免疫功能指标变化情况分为治疗有效组(n=68)和无效组(n=30)。患者出院后均行12~36个月的随访,根据患者预后情况分为预后良好组(n=63)和预后不良组(n=35)。对比对照组和肺癌组/有效组和无效组/预后FGL1、ANXA11表达差异;ROC分析FGL1、ANXA11单一及联合检测对肺癌手术患者临床效用评估价值;Spearman分析FGL1、ANXA11表达与预后的关系。结果肺癌组FGL1和ANXA11表达水平明显高于对照组(均P<0.05);无效组FGL1和ANXA11表达水平明显高于有效组(均P<0.05);ROC结果显示FGL1、ANXA11单独及联合检测对肺癌手术患者效用评估的曲线线下面积分别为0.928,并且联合检测具有较高的特异性(95.59%)以及敏感度(90.00%),诊断价值显著高于单独检测ROC曲线线下面积(均P<0.05);预后不良组FGL1和ANXA11表达水平明显高于预后良好组(均P<0.05);Spearman相关性结果显示FGL1、ANXA11与肺癌手术患者预后呈显著正相关(r=0.771、0.793,均P<0.05)。结论肺癌患者的FGL1与ANXA11表达水平高于健康人,治疗无效者高于有效者,预后不良者高于良好者,二者单独及联合检测对患者手术治疗效用评估均有价值,且其与预后相关性显著。展开更多
基金ACKNOWLEDGMENT This work was supported by the National Natural Science Foundation of China （No.20904047）.
文摘Elastic behaviors of protein-like chains are investigated by Pruned-Enriched-Rosenbluth method and modified orientation-dependent monomer-monomer interactions model. The protein-like chain is pulled away from the attractive surface slowly with elastic force acting on it. Strong adsorption interaction and no adsorption interaction are both considered. We calculate the characteristic ratio and shape factor of protein-like chains in the process of elongation. The conformation change of the protein-like chain is well depicted. The shape of chain changes from “rod” to “sphere” at the beginning of elongation. Then, the shape changes from “sphere” to “rod”. In the end, the shape becomes a “sphere” as the chain leaves away from the surface. In the meantime, we discuss average Helmoholtz free energy per bond, average energy per bond, average adsorbed energy per bond, average α-helical energy per bond, average β-sheet energy per bond and average contact energy per bond. On the other hand, elastic force is also studied. It is found that elastic force has a long plateau during the tensile elongation when there exists adsorption interaction. This result is consistent with SMFS experiment of general polymers. Energy contribution to elastic force and contact energy contribution to elastic force are both discussed. These investigations can provide some insights into the elastic behaviors of adsorbed protein chains.
基金supported by the National Natural Science Foundation of China(No.20904047)
文摘The effect of channel-protein interaction on the translocation of a protein-like chain through a finite channel under certain electric field was studied by using dynamical Monte Carlo simulations. The interior behavior of chain conformation under different interactions was investigated, such as the number of monomers outside of channel nout, monomers inside of channel nm, mean-square radius of gyration 〈 S2 〉 and the average energy 〈U〉. It shows that with strong attractive interaction, the translocation is more difficult than moderate interaction. At the same time, the dependence of translocation time with different interactions shows that moderate repulsive interaction （εcp = 0.5） accelerates the translocation. Although the waiting time for successful translocation of εep = 1.0 is the longest, the average translocation time is not very large. It is far smaller than that of εep = -1.0. The probability distributions of translocation time p（t＇） and the probability distributions of three duration times p（t1＇）, p（t2＇） and p（t3＇） were all discussed. Log-normal distributions are found. All these findings will strengthen the understanding of protein translocation.
基金This work was supported by the National Natural Science Foundation of China(No.31901515).
文摘MicroRNAs(miRNAs)are endogenous small non-coding RNAs that play an important role in post-transcriptional gene regulation in plants and animals by targeting messenger RNAs(mRNAs)for cleavage or repressing translation of specific mRNAs.The first miRNA identified in plants,miRNA156(miR156),targets the SQUAMOSA promoter-binding protein-like(SPL)transcription factors,which play critical roles in plant phase transition,flower and plant architecture,and fruit development.We identified multiple copies of MIR156 and SPL in the rice,Brachypodium,sorghum,maize,and foxtail millet genomes.Sequence and chromosomal synteny analysis showed that both MIR156s and SPLs are conserved across species in the grass family.Analysis of expression data of the SPLs in eleven juvenile and adult rice tissues revealed that four non-miR156-targeted genes were highly expressed and three miR156-targeted genes were only slightly expressed in all tissues/developmental stages.The remaining SPLs were highly expressed in the juvenile stage,but their expression was lower in the adult stage.It has been proposed that under strong selective pressure,non-miR156-targeted mRNA may be able to re-structure to form a miRNAresponsive element.In our analysis,some non-miR156-targeted SPLs(SPL5/8/10)had gene structure and gene expression patterns similar to those of miR156-targeted genes,suggesting that they could diversify into miR156-targeted genes.DNA methylation profiles of SPLs and MIR156s in different rice tissues showed diverse methylation patterns,and hypomethylation of non-CG sites was observed in rice endosperm.Our findings suggested that MIR156s and SPLs had different origination and evolutionary mechanisms:the SPLs appear to have resulted from vertical evolution,whereas MIR156s appear to have resulted from strong evolutionary selection on mature sequences.
基金The Zhejiang Provincial Natural Science Foundation of China,No.LQ17H160005.
文摘BACKGROUND Rearrangements of the anaplastic lymphoma kinase(ALK)gene(ALK-positive)represent an oncogenic driver in approximately 3%-5%of non-small-lung cancer(NSCLC)patients.Sarcoidosis is a multisystem disease,and its reported incidence in Asia is 1 or less per 100000 people per year.The co-occurrence of sarcoidosis and ALK-positive NSCLC is rare,and ALK-positive lung cancer is likely to spread quickly.Therefore,the co-occurrence of sarcoidosis is more easily misdiagnosed as metastatic lung cancer by radiological examination.CASE SUMMARY A 50-year-old man had a nodule in the left superior lobe,many small nodules in left superior and right lungs,and enlarged bilateral hilar,mediastinal,and right supraclavicular lymph nodes.Computed tomography-guided pulmonary biopsy of the nodule in the left superior lobe revealed echinoderm microtubuleassociated protein-like 4 gene-ALK positive NSCLC with concomitant noncaseating granuloma.This patient was treated with crizotinib.Thirty days later,a chest computed tomography scan revealed a dramatic decrease in the size of the left superior lobe nodule;however,the lesions in the right lung progressed.The right supraclavicular lymph nodes showed granulomas,and no tumor cells were identified in the specimens. The angiotensin-converting enzyme level was high.After 1 wk of methylprednisolone treatment, a significant response of all lesionswas revealed. Following radical resection of the lung cancer, noncaseatinggranulomas were observed in both lung tissues and lymph nodes, which resultedin a diagnosis of echinoderm microtubule-associated protein-like 4-ALK positiveNSCLC accompanied with sarcoidosis.CONCLUSIONOur experience illustrates that pathological evidence is needed to confirmmetastatic disease, especially when some suspected metastatic lesions arenegative for malignancy.
文摘BACKGROUND It is not uncommon to develop viral encephalitis.Epidemic Japanese B encephalitis infection combined with contactin-associated protein-like 2(CASPR-2)antibody-positive autoimmune encephalitis has not been reported at present.In clinical work,we need to consider more options.CASE SUMMARY A 32-year-old male worker presented with headache,fever and call-unresponsive presentation.Complete cranial magnetic resonance image showed symmetrical abnormal signals in bilateral medial temporal lobe,bilateral thalamus and basal ganglia.Improved lumbar puncture showed that cerebrospinal fluid protein and cell count increased significantly.Viral encephalitis was considered,and the patient's consciousness still increased rapidly after antiviral treatment.Further detection of Cerebrospinal fluid Japanese B encephalitis virus Polymerase Chain Reaction positive,serum autoimmune encephalitis antibody showed CASPR-2 antibody positive(1:320),the patient's condition gradually improved after plasma exchange treatment.3 mo later,the serum CASPR-2 antibody was negative and the patient's condition was stable.CONCLUSION This article reports the world’s first case of Epidemic Japanese B encephalitis infection combined with CASPR-2 antibody-positive autoimmune encephalitis,with a view to raising awareness.
基金the Scientific Research Foundation of Education Committee of Hunan (07C713 and 07C708)
文摘Objective： To clone and construct the recombinant plasmid containing ATP synthase lipid-binding protein-like protein gene of Schistosomajaponicum,（SjAslp） and transfer it into mammalian cells to express the objective protein. Methods： By polymerase chain reaction （PCR） technique, SjAslp was amplified from the constructed recombinant plasmid pBCSK＋/SjAslp, and inserted into cloning vector pUCm-T. Then, SjAslp was subcloned into an eukaryotic expression vector pcDNA3.1（＋）. After identifying it by PCR, restrictive enzymes digestion and DNA sequencing, the recombinant plasmid was transfected into HeLa cells using electroporation, and the expression of the recombinant protein was analyzed by immunocytochemical assay. Results： The specific gene fragment of 558 bp was successfully amplified. The DNA vaccine of SjAslp was successfully constructed. Immunocytochemical assay showed that SjAslp was expressed in the cytoplasm of HeLa cells. Conclusion： SjAslp gene can be expressed in eukaryotic system, which lays the foundation for development of the SjAslp DNA vaccine against schitosomiasis.
基金Project supported by the National Natural Science Foundation of China （Grant No. 20904047）, the Science and Technology Planning Project of Zhejiang Province, China （Grant No. 20100022）, and the Natural Science Foundation of Zhejiang Province, China （Grant No. Y6110304）.
文摘We study the translocation of a protein-like chain through a finite cylindrical channel using the pruned-enriched Rosenbluth method （PERM） and the modified orientation-dependent monomer-monomer interaction （ODI） model. Attractive channels （εcp = -2.0, -1.0, -0.5）, repulsive chanaels （εcp： 0.5, 1.0, 2.0）, and a neutral channel （εcp =- 0） are discussed. The results of the chain dimension and the energy show that Z0 ： 1.0 is an important case to distinguish the types of the channels. For the strong attractive channel, more contacts form during the process of translocation. It is also found that an external force is needed to drive the chain outside of the channel with the strong attraction. While for the neutral, the repulsive, and the weak attractive channels, the translocation is spontaneous.
基金The research was financially supported by the National Natural Science Foundation of China(Nos.20174036,20274040)the Natural Science Foundation of Zhejiang Province(No.R404047).
文摘The conformational properties and elastic behaviors of protein-like single chains in the process of tensileelongation were investigated by means of Monte Carlo method.The sequences of protein-like single chains contain two typesof residues:hydrophobic(H)and hydrophilic(P).The average conformations and thermodynamics statistical properties ofprotein-like single chains with various elongation ratio λ were calculated.It was found that the mean-square end-to-enddistance<R^2>(?).increases with elongation ratio λ.The tensor eigenvalues ratio of<L_2~2>:<L_1~2>decreases with elongationratio λ for short(HP)_x protein-like polymers,however,the ratio of<L_3~2>:<L_1~2>increases with elongation ratio λ,especially for long (H)_x sequence.Average energy per bond increases with elongation ratio λ,especially for (H)_xprotein-like single chains.Helmholtz free energy per bond also increases with elongation ratio λ.Elastic force(f),energycontribution to force(f_U)and entropy contribution to force(fs)for different protein-like single chains were also calculated.These investigations may provide some insights into elastic behaviors of proteins.
文摘目的:探究急性大血管闭塞性脑梗死(acute ischemic stroke due to large vessel occlusion,AIS-LVO)机械取栓的预后影响因素,构建列线图模型。方法:选取2020年1月至2023年8月保定市第一中心医院166例AIS-LVO患者,均接受机械取栓,根据取栓后3个月改良Rankin量表评分(modified rankin scale,mRS)分组,比较两组一般资料及血清视锥蛋白样蛋白-1(visininlike protein-1,VILIP-1)、硫氧还蛋白1(thioredoxin-1,Trx1)水平,采用Logistic回归方程、极端梯度提升(extreme gradient boosting,XGBoost)算法对AIS-LVO患者预后的相关因素进行筛选,构建列线图模型,采用受试者工作特征曲线(ROC)及曲线下面积(AUC)分析列线图模型的区分度,采用Bootstrap自抽样法,对数据集进行1000次重抽样的自举验证,进行内部验证并绘制校准曲线,采用决策曲线分析列线图模型的临床有效性。结果:预后不良组血清VILIP-1、入院时美国国立卫生研究院卒中量表(the national institutes of health stroke scale,NIHSS)评分高于预后良好组,Trx1、入院时Alberta卒中项目早期CT(alberta stroke project early CT,ASPECT)评分及CTA评分≥2级比例低于预后良好组(P<0.05);血清VILIP-1、Trx1、CTA评分≥2级及入院时NIHSS评分、ASPECT评分均是AIS-LVO机械取栓预后不良的影响因素(P<0.05);XGBoost算法显示,入院时NIHSS评分的SHAP平均值最高,其次为入院时ASPECT评分、VILIP-1、Trx1及CTA评分≥2级;采用Bootstrap自抽样法对列线图模型进行内部验证,列线图模型的AUCROC为0.927;校准曲线显示,模型的校准曲线观测值和预测值的重合度较好,经Hosmer-Lemeshow优度检验证实预测偏差不存在统计学意义(χ^(2)=5.411,P=0.736);决策曲线显示,列线图模型在0.0~1.0具有明显的正向净收益。结论:联合应用入院时NIHSS评分、ASPECT评分、CTA评分≥2级及血清VILIP-1、Trx1预测AIS-LVO机械取栓预后效能更佳,可帮助临床学者及早识别高危人群,减少预后不良发生。
文摘目的探究纤维蛋白原样蛋白1(fibrinogen-like protein 1,FGL1)和膜联蛋白A11(annexin A11,ANXA11)对肺癌手术患者效用评估价值及与预后的相关性。方法将本院2020年1月至2023年12月接诊的98例肺癌患者纳入肺癌组,另选取98例健康人作为对照组。受试者入院后分别于空腹状态下采取6 mL肘静脉血,采用ELISA法检测血清ANXA11水平,采用实时荧光定量PCR技术检测ANXA11表达水平。根据术后1个月肿瘤标志物变化、影像学表现和免疫功能指标变化情况分为治疗有效组(n=68)和无效组(n=30)。患者出院后均行12~36个月的随访,根据患者预后情况分为预后良好组(n=63)和预后不良组(n=35)。对比对照组和肺癌组/有效组和无效组/预后FGL1、ANXA11表达差异;ROC分析FGL1、ANXA11单一及联合检测对肺癌手术患者临床效用评估价值;Spearman分析FGL1、ANXA11表达与预后的关系。结果肺癌组FGL1和ANXA11表达水平明显高于对照组(均P<0.05);无效组FGL1和ANXA11表达水平明显高于有效组(均P<0.05);ROC结果显示FGL1、ANXA11单独及联合检测对肺癌手术患者效用评估的曲线线下面积分别为0.928,并且联合检测具有较高的特异性(95.59%)以及敏感度(90.00%),诊断价值显著高于单独检测ROC曲线线下面积(均P<0.05);预后不良组FGL1和ANXA11表达水平明显高于预后良好组(均P<0.05);Spearman相关性结果显示FGL1、ANXA11与肺癌手术患者预后呈显著正相关(r=0.771、0.793,均P<0.05)。结论肺癌患者的FGL1与ANXA11表达水平高于健康人,治疗无效者高于有效者,预后不良者高于良好者,二者单独及联合检测对患者手术治疗效用评估均有价值,且其与预后相关性显著。
