The insect is the animal protein feed resource potential, the rapid repro- duction, large quantity, high protein content, and most species of insects can be used as livestock feed utilization, development of insect as...The insect is the animal protein feed resource potential, the rapid repro- duction, large quantity, high protein content, and most species of insects can be used as livestock feed utilization, development of insect as feed resources, to pro- mote the development of China＇s animal husbandry and feed industry, has important significance.展开更多
Background Addressing the shortage of high-quality protein resources,this study was conducted to investigate the effects of replacing soybean meal(SBM)with different levels of enzymolysis-fe rmentation compound protei...Background Addressing the shortage of high-quality protein resources,this study was conducted to investigate the effects of replacing soybean meal(SBM)with different levels of enzymolysis-fe rmentation compound protein feed(EFCP)in the diets of growing—finishing pigs,focusing on growth perfo rmance,nutrients digestibility;carcass traits,and meat quality.Methods Sixty DLY(Durdcx LandracexYorkshire)pigs with an initial body weight of 42.76±2.05 kg were assigto 5 diietary treatments in a 2×2+1 factorial design.These dietary treatments included a corn-soybean meal diet(CON),untreated compound protein feed(UCP)substitution 50%(U50)and 100%SBM(U100)diets,and EFCP substitution 50%(EF50)and 100%SBM(EF100)diets.Each treatment had 6 pens(replicates)with 2 pigs per pen,and the experiment lasted 58 d,divided into phaseⅠ(1-28 d)and phaseⅡ(29-58 d).Following phaseⅠ,only the CON,U50,and EF50 groups were continued for phaseⅡ,each with 5 replicate pens.On d 59,a total of 15 pigs(1 pig/pen,5pens/treatment)were euthanized.Results During phaseⅠ,the EF50 group had a higher average daily gain(ADG)in pigs(P<0.05)compared to the CON group,whereas the U50 group did not have a significant difference.As the substitution ratio of UCP and EFCP increased in phaseⅠ,there was a noticeable reduction in the final body weight and ADG(P<0.05),along with an increase in the feed-to-gain ratio(F/G)(P<0.05).In phaseⅡ,there were no significant differences in growth performance among the treatment groups,but EF50 increased the apparent digestibility of several nutrients(including dry matter,crude protein,crude fiber,acid detergent fiber,ash,gross energy)compared to U50.The EF50 group also exhibited significantly higher serum levels of neuropeptide Y and ghrelin compared to the CON and U50 groups(P<0.05),Moreover,the EF50 group had higher carcass weight and carcass length than those in the CON and U50groups(P<0.05),with no significant difference in meat quality.Conclusions The study findings suggest that replacing 50%SBM with EFCP during the growing-finishing period can improve the growth performance,nutrient digestibility,and carcass traits of pigs without compromising meat quality This research offers valuable insights into the modification of unconventional plant protein meals and developing alternatives to SBM.展开更多
[Objectives] This study was conducted to explore the effects of new fly maggot protein feed on the mRNA expression of genes related to the TOR signaling pathway in loaches(Misgurnus anguillicaudatus). [Methods] Two ki...[Objectives] This study was conducted to explore the effects of new fly maggot protein feed on the mRNA expression of genes related to the TOR signaling pathway in loaches(Misgurnus anguillicaudatus). [Methods] Two kinds of test feed with equal nitrogen and energy were prepared by replacing 60% of the fish meal in the control group with the new fly maggot protein feed, i.e., Diet1(control group) and Diet2(60% fish meal replacement group). The feeding experiment was carried out in an indoor circulating water system, and the breeding period was 60 d. [Results] For the livers, the mRNA levels of TOR and 4EBP1 in the Diet2 group were significantly higher than those in the Diet1 group(P<0.05), while the expression of 4 EBP2 was lower than in the Diet1 group(P<0.05);and as to the muscles, the mRNA levels of TOR and 4EBP1 in the Diet2 group were significantly lower than those in the Diet1 group(P<0.05), while there was no significant change in the mRNA level of 4EBP2 between the two groups. [Conclusions] The replacement of fish meal by fly maggot cultures affected the mRNA expression of TOR, 4EBP1 and 4EBP2 in loach livers and muscles.展开更多
This study aimed to elucidate the effect of a pellet feed containing fermented proteins on pond-raised Chinese mitten crabs,Eriocheir sinensis in a 7-month crab feeding experiment in Gucheng Lake,Jiangsu province,Chin...This study aimed to elucidate the effect of a pellet feed containing fermented proteins on pond-raised Chinese mitten crabs,Eriocheir sinensis in a 7-month crab feeding experiment in Gucheng Lake,Jiangsu province,China.The results showed that fermented protein could significantly increase mean weight gain,crab size and total yield compared with control group.Moreover,the mean weight of male crabs and the number of large-sized male crabs improved over those of the control group.However,feed efficiency ratio did not differ significantly between the two groups.The amino acid content in the muscles of the female crabs of both the groups was higher than that in the muscles of the male crabs.The present study suggests that fermented compound protein may be one kind of potential protein sources for E.sinensis.展开更多
Background: Residual feed intake(RFI) is an inheritable measure of feed efficiency that is independent on level of production. However, physiological and metabolic mechanisms underlying divergent RFI are not fully elu...Background: Residual feed intake(RFI) is an inheritable measure of feed efficiency that is independent on level of production. However, physiological and metabolic mechanisms underlying divergent RFI are not fully elucidated.This study was conducted to investigate dietary nitrogen(N) partitioning and microbial protein synthesis in lactating dairy cows divergent in phenotypic RFI.Results: Thirty Holstein dairy cows(milk yield = 35.3 ± 4.71 kg/d;milk protein yield = 1.18 ± 0.13 kg/d;mean ± standard deviation) were selected for the experiment to derive RFI. After the RFI measurement period of 50 d, the 10 lowest RFI cows and 8 highest RFI cows were selected. The low RFI cows had lower dry matter intake(DMI, P < 0.05) than the high RFI cows, but they produced similar energy-corrected milk. The ratios of milk to DMI(1.41 vs. 1.24, P < 0.01) and energy-corrected milk to DMI(1.48 vs. 1.36, P < 0.01) were greater in low RFI cows than those in the high RFI cows. The low RFI cows had lower milk urea nitrogen than that in the high RFI cows(P = 0.05). Apparent digestibility of nutrients did not differ between two groups(P > 0.10). Compared with high RFI animals, the low RFI cows had a lower retention of N(5.72 vs. 51.4 g/d, P < 0.05) and a higher partition of feed N to milk N(29.7% vs. 26.5%, P < 0.05).Conclusions: The results suggest that differences in N partition, synthesis of microbial protein, and utilization of metabolizable protein could be part of the mechanisms associated with variance in the RFI.展开更多
Slow-release non-protein nitrogen feed has a large market demand. It has a long research history, but its production technology needs further improvement in order to realize the industrial production of slow-release n...Slow-release non-protein nitrogen feed has a large market demand. It has a long research history, but its production technology needs further improvement in order to realize the industrial production of slow-release non-protein nitrogen extruded feed. By designing the best formula and using chelating and emulsifying process, the slow-release non-protein nitrogen extruded feed additives were produced. This product increases milk yield and improves milk quality, thus increasing economic efficiency.展开更多
The effect of fishmeal quality on growth,feed conversion ratio and protein digestibility was studied in a growth experiment with shrimp and red seabream.The shrimp and fish were fed three diets varing in the quality o...The effect of fishmeal quality on growth,feed conversion ratio and protein digestibility was studied in a growth experiment with shrimp and red seabream.The shrimp and fish were fed three diets varing in the quality of the fishmeal used in the respective feeds: low quality fish meal,good quality fish meal,and Peru fish meal.The experiment lasted for 2 months.The shrimp and fish fed the feed with good quality fish meal showed significantly better feed conversion ratio,weight gain rate,protein digestibility than the other groups.展开更多
研究旨在应用康奈尔净碳水化合物-蛋白质体系(cornell net carbohydrate and protein system,CNCPS)比较新疆地区几种棉源饲料的营养价值。基于CNCPS和预测能值指标,对棉籽蛋白、棉籽饼粕等7种新疆典型棉源饲料展开营养特性评估。结果表...研究旨在应用康奈尔净碳水化合物-蛋白质体系(cornell net carbohydrate and protein system,CNCPS)比较新疆地区几种棉源饲料的营养价值。基于CNCPS和预测能值指标,对棉籽蛋白、棉籽饼粕等7种新疆典型棉源饲料展开营养特性评估。结果表明:棉籽蛋白粗蛋白(CP)含量为51.09%DM,过瘤胃处理使缓释蛋白的中速降解真蛋白质(PB_(2))、慢速降解真蛋白质(PB_(3))分别提升13.38%、2.56%,可替代豆粕构建高效过瘤胃蛋白(rumen-undegradable protein,RUP)体系,证实其作为过瘤胃蛋白源的营养价值;棉籽以18.21%DM的粗脂肪(EE)含量和85.26%的总可消化养分(TDN),成为育肥期能量补充的理想选择;棉壳因87.45%DM的中性洗涤纤维(NDF)含量使代谢能(ME)显著降低(1.52 MJ/kg),可将其作为反刍动物日粮中的纤维组分。展开更多
An 8-week feeding experiment was conducted in an indoor recirculation seawater system to investigate the effects of partial replacement of dietary fishmeal with proteins from five sources on the growth performance and...An 8-week feeding experiment was conducted in an indoor recirculation seawater system to investigate the effects of partial replacement of dietary fishmeal with proteins from five sources on the growth performance and feed utilization of Sebastes schlegeli. Six isonitrogenous and isoenergetic diets were formulated using fishmeal(FM, the control) as sole protein source, or proteins from five sources including poultry by-product meal(PBM), meat and bone meal(MBM), soybean meal(SBM), cottonseed meal(CSM) and canola meal(CNM). Fifteen percent of the crude protein provided by fish meal was replaced, respectively. The results showed that the differences in specific growth rate(SGR) and survival rate(SR) among fish fed PBM, MBM, SBM, CSM and whole FM diets were not significant. However, SGR and SR of fish fed CNM diet was significantly lower than that of other treatments. Feeding rate, feed conversion, nutrient retention showed similar patterns to that of growth. Fish fed CSM and CNM showed significantly lower apparent digestibility coefficient(ADC) of dry matter and gross energy than those fed others while fish fed CNM showed lower ADC of crude protein than those fed others(P<0.05). These results suggested that it was feasible to substitute 15% dietary protein provided by fishmeal with PBM, MBM, SBM and CSM, respectively, but not with CNM as the replacement with CNM reduced fish growth and feed utilization.展开更多
Heavy metals, including cadmium (Cd), are widespread pollutants of great environmental concern because of their ac- cumulation and toxicity. Environmental conditions can affect the accumulation and elimination of Cd i...Heavy metals, including cadmium (Cd), are widespread pollutants of great environmental concern because of their ac- cumulation and toxicity. Environmental conditions can affect the accumulation and elimination of Cd in organisms. The aim of this study is to understand the respective responses of metallothioneins (MTs), P-glycoprotein (P-gp), and heat shock protein 70 (hsp70) genes in the mantle and digestive gland of the oyster Crassostrea gigas exposed to 10 g L 1 Cd for 28 days, followed by a depura- tion period of 35 days under different feeding strategies. The MT expression in the digestive gland was higher than in mantle, and was induced in a fluctuating pattern throughout the time of the whole experiment when the oyster was exposed to Cd. The results indicated an enhanced MT activity is required for Cd detoxification. Expression levels of P-gp in the mantle of Cd-exposed oysters fed with algae increased in the first two weeks of the accumulation phase. Then the induction weakened, but the expression still in- creased significantly compared with the control group fed without algae. The P-gp levels in the digestive gland were under-regulated during both the contamination and decontamination periods except in the group fed with Dicrateria inornata at day 0.083. The in- duction of P-gp is possibly related to the important role of pumping Cd out of the cell, while a major implication of P-gp expression inhibition would be a disruption of the protein synthesis. Hsp70 expression exhibited an overall decreasing trend. The highest relative hsp70 inhibition occurred during day 42 to day 63, with approximately 4-fold and 10-fold lower hsp70 levels as compared to the C.gigas feeding with no algae in the mantle and digestive gland, respectively. The induction of hsp70 may be explained by the re- folding of protein in presence of Cd, followed by the inhibition of hsp70 when the protein synthesis was disrupted.展开更多
基金Supported by the Planned Project for the Innovation and Entrepreneurship Training of College Students in China~~
文摘The insect is the animal protein feed resource potential, the rapid repro- duction, large quantity, high protein content, and most species of insects can be used as livestock feed utilization, development of insect as feed resources, to pro- mote the development of China＇s animal husbandry and feed industry, has important significance.
基金supported by the Major Science and Technology Program of Sichuan Province(No.2021ZDZX0009)Key Research and Development Program of Sichuan Province(No.2020YFN0147)。
文摘Background Addressing the shortage of high-quality protein resources,this study was conducted to investigate the effects of replacing soybean meal(SBM)with different levels of enzymolysis-fe rmentation compound protein feed(EFCP)in the diets of growing—finishing pigs,focusing on growth perfo rmance,nutrients digestibility;carcass traits,and meat quality.Methods Sixty DLY(Durdcx LandracexYorkshire)pigs with an initial body weight of 42.76±2.05 kg were assigto 5 diietary treatments in a 2×2+1 factorial design.These dietary treatments included a corn-soybean meal diet(CON),untreated compound protein feed(UCP)substitution 50%(U50)and 100%SBM(U100)diets,and EFCP substitution 50%(EF50)and 100%SBM(EF100)diets.Each treatment had 6 pens(replicates)with 2 pigs per pen,and the experiment lasted 58 d,divided into phaseⅠ(1-28 d)and phaseⅡ(29-58 d).Following phaseⅠ,only the CON,U50,and EF50 groups were continued for phaseⅡ,each with 5 replicate pens.On d 59,a total of 15 pigs(1 pig/pen,5pens/treatment)were euthanized.Results During phaseⅠ,the EF50 group had a higher average daily gain(ADG)in pigs(P<0.05)compared to the CON group,whereas the U50 group did not have a significant difference.As the substitution ratio of UCP and EFCP increased in phaseⅠ,there was a noticeable reduction in the final body weight and ADG(P<0.05),along with an increase in the feed-to-gain ratio(F/G)(P<0.05).In phaseⅡ,there were no significant differences in growth performance among the treatment groups,but EF50 increased the apparent digestibility of several nutrients(including dry matter,crude protein,crude fiber,acid detergent fiber,ash,gross energy)compared to U50.The EF50 group also exhibited significantly higher serum levels of neuropeptide Y and ghrelin compared to the CON and U50 groups(P<0.05),Moreover,the EF50 group had higher carcass weight and carcass length than those in the CON and U50groups(P<0.05),with no significant difference in meat quality.Conclusions The study findings suggest that replacing 50%SBM with EFCP during the growing-finishing period can improve the growth performance,nutrient digestibility,and carcass traits of pigs without compromising meat quality This research offers valuable insights into the modification of unconventional plant protein meals and developing alternatives to SBM.
基金Supported by General Project of Natural Science Research in Higher Education Institutions in Jiangsu Province(18KJD240001)Xuzhou Science and Technology Planning Project(KC19200)2018“Qinglan Project”of Jiangsu Higher Education Institutions。
文摘[Objectives] This study was conducted to explore the effects of new fly maggot protein feed on the mRNA expression of genes related to the TOR signaling pathway in loaches(Misgurnus anguillicaudatus). [Methods] Two kinds of test feed with equal nitrogen and energy were prepared by replacing 60% of the fish meal in the control group with the new fly maggot protein feed, i.e., Diet1(control group) and Diet2(60% fish meal replacement group). The feeding experiment was carried out in an indoor circulating water system, and the breeding period was 60 d. [Results] For the livers, the mRNA levels of TOR and 4EBP1 in the Diet2 group were significantly higher than those in the Diet1 group(P<0.05), while the expression of 4 EBP2 was lower than in the Diet1 group(P<0.05);and as to the muscles, the mRNA levels of TOR and 4EBP1 in the Diet2 group were significantly lower than those in the Diet1 group(P<0.05), while there was no significant change in the mRNA level of 4EBP2 between the two groups. [Conclusions] The replacement of fish meal by fly maggot cultures affected the mRNA expression of TOR, 4EBP1 and 4EBP2 in loach livers and muscles.
基金This study was supported by Science and Technology Planning Project of Tianjin City,China(2018YFD0500603).
文摘This study aimed to elucidate the effect of a pellet feed containing fermented proteins on pond-raised Chinese mitten crabs,Eriocheir sinensis in a 7-month crab feeding experiment in Gucheng Lake,Jiangsu province,China.The results showed that fermented protein could significantly increase mean weight gain,crab size and total yield compared with control group.Moreover,the mean weight of male crabs and the number of large-sized male crabs improved over those of the control group.However,feed efficiency ratio did not differ significantly between the two groups.The amino acid content in the muscles of the female crabs of both the groups was higher than that in the muscles of the male crabs.The present study suggests that fermented compound protein may be one kind of potential protein sources for E.sinensis.
基金supported by grants from the National Natural Science Foundation of China(No.31872380)the China Agricultural Research System(Beijing,China No.CARS-36)
文摘Background: Residual feed intake(RFI) is an inheritable measure of feed efficiency that is independent on level of production. However, physiological and metabolic mechanisms underlying divergent RFI are not fully elucidated.This study was conducted to investigate dietary nitrogen(N) partitioning and microbial protein synthesis in lactating dairy cows divergent in phenotypic RFI.Results: Thirty Holstein dairy cows(milk yield = 35.3 ± 4.71 kg/d;milk protein yield = 1.18 ± 0.13 kg/d;mean ± standard deviation) were selected for the experiment to derive RFI. After the RFI measurement period of 50 d, the 10 lowest RFI cows and 8 highest RFI cows were selected. The low RFI cows had lower dry matter intake(DMI, P < 0.05) than the high RFI cows, but they produced similar energy-corrected milk. The ratios of milk to DMI(1.41 vs. 1.24, P < 0.01) and energy-corrected milk to DMI(1.48 vs. 1.36, P < 0.01) were greater in low RFI cows than those in the high RFI cows. The low RFI cows had lower milk urea nitrogen than that in the high RFI cows(P = 0.05). Apparent digestibility of nutrients did not differ between two groups(P > 0.10). Compared with high RFI animals, the low RFI cows had a lower retention of N(5.72 vs. 51.4 g/d, P < 0.05) and a higher partition of feed N to milk N(29.7% vs. 26.5%, P < 0.05).Conclusions: The results suggest that differences in N partition, synthesis of microbial protein, and utilization of metabolizable protein could be part of the mechanisms associated with variance in the RFI.
基金funded by the Higher Vocational Colleges and Higher Junior Colleges Research Project of Heilongjiang Provincial Education Department (11515077)
文摘Slow-release non-protein nitrogen feed has a large market demand. It has a long research history, but its production technology needs further improvement in order to realize the industrial production of slow-release non-protein nitrogen extruded feed. By designing the best formula and using chelating and emulsifying process, the slow-release non-protein nitrogen extruded feed additives were produced. This product increases milk yield and improves milk quality, thus increasing economic efficiency.
文摘The effect of fishmeal quality on growth,feed conversion ratio and protein digestibility was studied in a growth experiment with shrimp and red seabream.The shrimp and fish were fed three diets varing in the quality of the fishmeal used in the respective feeds: low quality fish meal,good quality fish meal,and Peru fish meal.The experiment lasted for 2 months.The shrimp and fish fed the feed with good quality fish meal showed significantly better feed conversion ratio,weight gain rate,protein digestibility than the other groups.
文摘研究旨在应用康奈尔净碳水化合物-蛋白质体系(cornell net carbohydrate and protein system,CNCPS)比较新疆地区几种棉源饲料的营养价值。基于CNCPS和预测能值指标,对棉籽蛋白、棉籽饼粕等7种新疆典型棉源饲料展开营养特性评估。结果表明:棉籽蛋白粗蛋白(CP)含量为51.09%DM,过瘤胃处理使缓释蛋白的中速降解真蛋白质(PB_(2))、慢速降解真蛋白质(PB_(3))分别提升13.38%、2.56%,可替代豆粕构建高效过瘤胃蛋白(rumen-undegradable protein,RUP)体系,证实其作为过瘤胃蛋白源的营养价值;棉籽以18.21%DM的粗脂肪(EE)含量和85.26%的总可消化养分(TDN),成为育肥期能量补充的理想选择;棉壳因87.45%DM的中性洗涤纤维(NDF)含量使代谢能(ME)显著降低(1.52 MJ/kg),可将其作为反刍动物日粮中的纤维组分。
基金funded by National Key Basic Research Program (2009CB118702)partly by the Knowledge Innovation Program of the Chinese Academy of Sciences
文摘An 8-week feeding experiment was conducted in an indoor recirculation seawater system to investigate the effects of partial replacement of dietary fishmeal with proteins from five sources on the growth performance and feed utilization of Sebastes schlegeli. Six isonitrogenous and isoenergetic diets were formulated using fishmeal(FM, the control) as sole protein source, or proteins from five sources including poultry by-product meal(PBM), meat and bone meal(MBM), soybean meal(SBM), cottonseed meal(CSM) and canola meal(CNM). Fifteen percent of the crude protein provided by fish meal was replaced, respectively. The results showed that the differences in specific growth rate(SGR) and survival rate(SR) among fish fed PBM, MBM, SBM, CSM and whole FM diets were not significant. However, SGR and SR of fish fed CNM diet was significantly lower than that of other treatments. Feeding rate, feed conversion, nutrient retention showed similar patterns to that of growth. Fish fed CSM and CNM showed significantly lower apparent digestibility coefficient(ADC) of dry matter and gross energy than those fed others while fish fed CNM showed lower ADC of crude protein than those fed others(P<0.05). These results suggested that it was feasible to substitute 15% dietary protein provided by fishmeal with PBM, MBM, SBM and CSM, respectively, but not with CNM as the replacement with CNM reduced fish growth and feed utilization.
基金supported by the Earmarked Fund for Modern Agro-industry Technology Research System in Shandong Province (No. SDAIT-14)the major Agricultural Applied Technological Innovation Program in Shandong Province: Construction and Demonstration of Ecological Farming Model of Monomeric Triploid Oyster
文摘Heavy metals, including cadmium (Cd), are widespread pollutants of great environmental concern because of their ac- cumulation and toxicity. Environmental conditions can affect the accumulation and elimination of Cd in organisms. The aim of this study is to understand the respective responses of metallothioneins (MTs), P-glycoprotein (P-gp), and heat shock protein 70 (hsp70) genes in the mantle and digestive gland of the oyster Crassostrea gigas exposed to 10 g L 1 Cd for 28 days, followed by a depura- tion period of 35 days under different feeding strategies. The MT expression in the digestive gland was higher than in mantle, and was induced in a fluctuating pattern throughout the time of the whole experiment when the oyster was exposed to Cd. The results indicated an enhanced MT activity is required for Cd detoxification. Expression levels of P-gp in the mantle of Cd-exposed oysters fed with algae increased in the first two weeks of the accumulation phase. Then the induction weakened, but the expression still in- creased significantly compared with the control group fed without algae. The P-gp levels in the digestive gland were under-regulated during both the contamination and decontamination periods except in the group fed with Dicrateria inornata at day 0.083. The in- duction of P-gp is possibly related to the important role of pumping Cd out of the cell, while a major implication of P-gp expression inhibition would be a disruption of the protein synthesis. Hsp70 expression exhibited an overall decreasing trend. The highest relative hsp70 inhibition occurred during day 42 to day 63, with approximately 4-fold and 10-fold lower hsp70 levels as compared to the C.gigas feeding with no algae in the mantle and digestive gland, respectively. The induction of hsp70 may be explained by the re- folding of protein in presence of Cd, followed by the inhibition of hsp70 when the protein synthesis was disrupted.
