Melatonin(N-Acetyl-5-methoxytryptamine),an endogenously synthesized indoleamine,exerts pleiotropic effects in plant physiology by interacting with other phytohormones,thereby synergistically regulating plant growth,de...Melatonin(N-Acetyl-5-methoxytryptamine),an endogenously synthesized indoleamine,exerts pleiotropic effects in plant physiology by interacting with other phytohormones,thereby synergistically regulating plant growth,development,and stress responses.Recent research has also indicated that melatonin derivatives could be further developed as promising antifungal candidates.Given the significant roles of melatonin,considerable efforts have been devoted to studying its potent functions and underlying mechanisms.This review outlines recent advancements in understanding the roles of melatonin in regulating plant growth and its potential synergistic interactions with pathogens.Additionally,we present our perspectives aimed at elucidating the antifungal mechanisms of melatonin and its derivatives,which could facilitate the development of naturally sourced fungicides and offer innovative strategies for pesticide discovery.展开更多
Ozone(O_(3))pollution has elevated in China,threatening plants and crop production.Ethylenediurea(EDU)is a chemical alleviating O_(3)-induced phytotoxicities.This study aimed at revealing fluctuating O_(3) exposures e...Ozone(O_(3))pollution has elevated in China,threatening plants and crop production.Ethylenediurea(EDU)is a chemical alleviating O_(3)-induced phytotoxicities.This study aimed at revealing fluctuating O_(3) exposures effects on Youxian No 3(Phaseolus vulgaris)and Sukui No 4(Vigna angularis),two widely grown Chinese bean cultivars,and EDU role in mediating these effects.Plants were periodically treated with EDU(400 mg/L)or water and subjected to charcoal-filtered air(CF)or non-filtered ambient air enriched with an additional targeted O_(3) concentration of 40 ppb(NF40).with subsequent ambient or NF40 exposures.A 10-day exposure to NF40 increased photosynthetic rate(A)while decreasing the leaf intercellular CO_(2) concentration(Ci),but this effect was absent after moving plants to ambient air for two weeks.Moving previously CF-exposed plants to ambient air for two weeks also increased A,which was not linked with Ci but more related to stomatal conductance(gs).Following two one-week and two-week sequential exposures of all plants to NF40,with an intermediate exposure to ambient air,elevated O_(3) reduced chlorophylls(SPAD),A,gs,Ci,and transpiration and decoupled A-gs response.More O_(3) effectswere observed in plants treatedwithNF40 during each O_(3)-treatment cycle,compared to those exposed to CF during the first cycle.The former plants exhibited significantly decreased biomass and water content of leaves and stems but increased flowers biomass and water content.Some of the effects were cultivardependent,with Youxian showing more apparent trade-offs between vegetative growth and reproduction.EDU alleviated various negative O_(3) effects.展开更多
RNA interference(RNAi)has been used for agricultural insect pest control based on silencing of targeted insect genes.However,the effectiveness of RNAi and its applications in insect pest control remain challenging.Her...RNA interference(RNAi)has been used for agricultural insect pest control based on silencing of targeted insect genes.However,the effectiveness of RNAi and its applications in insect pest control remain challenging.Here we review factors that may affect the effectiveness of RNAi application,including the variability in RNAi efficacy among different insect species,a limited understanding of double-stranded RNA(dsRNA)uptake and systemic RNAi mechanisms,and the effective delivery of dsRNA in field conditions.Furthermore,we summarize recent progress in RNAi strategies for crop protection,discuss the advantages and disadvantages of RNAi-based insect control,and propose potential strategies to increase the effectiveness of RNAi in insect control.展开更多
RNA interference(RNAi)is a post-transcriptional gene-silencing technique induced by the introduction of double-stranded RNA(dsRNA)or small interfering RNA(siRNA)[1].RNAi-based strategies have been extensively applied ...RNA interference(RNAi)is a post-transcriptional gene-silencing technique induced by the introduction of double-stranded RNA(dsRNA)or small interfering RNA(siRNA)[1].RNAi-based strategies have been extensively applied in the treatment of human diseases and crop protection against insect pests[2-4].With the availability of the full genome sequences of major mosquito vectors,RNAi has become increasingly used as a novel means of mosquito control[5].展开更多
This review provides a summary of botanicals and plant strengtheners that have potential uses for disease and pest management in potato and tomato cultivation in African.We discuss their possible use to prevent major ...This review provides a summary of botanicals and plant strengtheners that have potential uses for disease and pest management in potato and tomato cultivation in African.We discuss their possible use to prevent major diseases and pests which infest potato and tomato,such as early and late blight,bacterial wilt,potato tuber moth,and tomato leafminer.There are several examples of the successful uses of botanicals for pathogen and pest control relevant for different African climatic conditions;however,most of these studies have been conducted in vitro and often lack field verification.Plant strengtheners(substances that induce and improve crop resistance,yield,and quality)are little studied and used in Africa in comparison to North America and Europe.The possible benefits of using botanicals and plant strengtheners instead of conventional pesticides are discussed here in relation to human health and the environment as well as their modes of action and accessibility to farmers.Lack of knowledge of the composition and active ingredients of extracts,environmental concerns,uncertainties regarding stability and formulation,lack of legislation and limited support from governments,hamper the development of botanicals and plant strengtheners for use in sustainable African agriculture.展开更多
Stripe rust,caused by Puccinia striiformis f.sp.tritici(Pst),is a devastating disease in wheat worldwide.Discovering and characterizing new resistance genes/QTL is crucial for wheat breeding programs.In this study,we ...Stripe rust,caused by Puccinia striiformis f.sp.tritici(Pst),is a devastating disease in wheat worldwide.Discovering and characterizing new resistance genes/QTL is crucial for wheat breeding programs.In this study,we fine-mapped and characterized a stripe rust resistance gene,YRAYH,on chromosome arm 5BL in the Chinese wheat landrace Anyuehong(AYH).Evaluations of stripe rust response to prevalent Chinese Pst races in near-isogenic lines derived from a cross of Anyuehong and Taichung 29 showed that YrAYH conferred a high level of resistance at all growth stages.Fine mapping using a large segregating population of 9748 plants,narrowed the YRAYH locus to a 3.7 Mb interval on chromosome arm 5BL that included 61 annotated genes.Transcriptome analysis of two NIL pairs identified 64 upregulated differentially expressed genes(DEGs)in the resistant NILs(NILs-R).Annotations indicated that many of these genes have roles in plant disease resistance pathways.Through a combined approach of fine-mapping and transcriptome sequencing,we identified a serine/threonine-protein kinase SRPK as a candidate gene underlying YrAYH.A unique 25 bp insertion was identified in the NILs-R compared to the NILs-S and previously published wheat genomes.An InDel marker was developed and co-segregated with YrAYH.Agronomic trait evaluation of the NILs suggested that YrAYH not only reduces the impact of stripe rust but was also associated with a gene that increases plant height and spike length.展开更多
The safety of crop protection products and their safe use is of paramount importance and requires that crop protection products are consistently marketed and used worldwide in the required registered quality.To meet t...The safety of crop protection products and their safe use is of paramount importance and requires that crop protection products are consistently marketed and used worldwide in the required registered quality.To meet these high-quality requirements,the highest demands must be placed on the production and purification of technical-grade crop protection products.In this context,impurity profiles are of utmost importance,as unknown impurities at higher than registered concentrations may pose new unknown toxicological risks to users,consumers,and the environment.Particularly high demands are placed on the production and purification of technical grade semisynthetic derivatives of fermentatively derived natural products to meet the required regulatory specifications,to ensure that relevant impurities do not exceed maximum allowable concentrations,to ensure the safe use of such products,and to eliminate any unknown product safety risks.The challenges associated with the manufacture and purification of Afidopyropen and the product safety risks associated with various qualities of technical grade Emamectin benzoate are discussed in detail.展开更多
Birds are a huge hazard to agriculture all around the world,causing harm to profitable field crops.Growers use a variety of techniques to keep them away,including visual,auditory,tactile,and olfactory deterrents. This...Birds are a huge hazard to agriculture all around the world,causing harm to profitable field crops.Growers use a variety of techniques to keep them away,including visual,auditory,tactile,and olfactory deterrents. This study presents a comprehensive overview of current bird repellant approaches used in agricultural contexts,as well as potential new ways. The bird repellent techniques include Internet of Things technology,Deep Learning,Convolutional Neural Network,Unmanned Aerial Vehicles,Wireless Sensor Networks and Laser biotechnology. This study’s goal is to find and review about previous approach towards repellent of birds in the crop fields using various technologies.展开更多
The increasing of highway engineering construction makes soil and water loss surrounding highway and also influences the growth of crops around, To control soil and water loss, new engineering measures are put forward...The increasing of highway engineering construction makes soil and water loss surrounding highway and also influences the growth of crops around, To control soil and water loss, new engineering measures are put forward to protect soil and water and prevent crops from being damaged.展开更多
Crop pests are a permanent threat to horticultural production. Several control methods are recommended for their control, including biological control. This method based on the use of natural enemies is not well estab...Crop pests are a permanent threat to horticultural production. Several control methods are recommended for their control, including biological control. This method based on the use of natural enemies is not well established. The objective of this study was to assess the diversity and abundance of natural enemies of crop pests. A survey of crops associated with pests was carried out on 144 fields in southern Senegal to measure the diversity and abundance of natural enemies of various crop pests, determine the native parasitoid complex and assess parasitism rates. Ecological indices were calculated to assess the diversity of natural enemies. A total of 25 natural enemy species were identified, including 15 parasitoids and 10 predators. Predatory Hemiptera and Parasitoid Hymenoptera were high biodiversity. The parasitism rates vary between 0 and 50% depending on the abundance of pests and parasitoid species. Knowledge of the diversity of natural enemies and the understanding of trophic interactions with pests are important in the development of biological crop protection in order to preserve resilience in agroecosystems.展开更多
World crop production requires highly-productive varieties of agricultural crops,which are resistant to pest organisms.Such varieties are also of great importance for the Uzbekistan.Their
The introduction of invasive insect pests across national borders has become a major concern in crop production. Accordingly, national plant protection organizations are challenge to reinforce their monitoring strateg...The introduction of invasive insect pests across national borders has become a major concern in crop production. Accordingly, national plant protection organizations are challenge to reinforce their monitoring strategies, which are hampered by the weight and size of inspection equipment, as well as the taxonomic extensiveness of interrupted species. Moreover, some insect pests that impede farmer productivity and profitability are difficult for researchers to address on time due to a lack of appropriate plant protection measures. Farmers’ reliance on synthetic pesticides and biocontrol agents has resulted in major economic and environmental ramifications. DNA barcoding is a novel technology that has the potential to improve Integrated Pest Management decision-making, which is dependent on the ability to correctly identify pest and beneficial organisms. This is due to some natural traits such as phenology or pesticide susceptibility browbeaten by IPM strategies to avert pest establishment. Specifically, Deoxyribonucleic acid (DNA) sequence information was applied effectively for the identification of some micro-organisms. This technology, DNA barcoding, allows for the identification of insect species by using short, standardized gene sequences. DNA barcoding is basically based on repeatable and accessible technique that allows for the mechanisation or automation of species discrimination. This technique bridges the taxonomic bio-security gap and meets the International Plant Protection Convention diagnostic standards for insect identification. This review therefore discusses DNA barcoding as a technique for insect pests’ identification and its potential application for crop protection.展开更多
Synthetic chemicals have been widely used in modern agriculture as fertilizer,plant growth regulators and pesticides.Although the chemical inputs improved the crop production,it is well known about their negative effe...Synthetic chemicals have been widely used in modern agriculture as fertilizer,plant growth regulators and pesticides.Although the chemical inputs improved the crop production,it is well known about their negative effects,such as environmental residues in water,soil and animals,pest resistance and resurgence and the residues in agricultural produces.The demands for safer food and more friendly environment are now more preferred by humankind in the world.Microbes have already become an alternate for chemical inputs in some aspects in agriculture.The microbes can produce very diverse volatile organic compounds(mVOCs)which play important roles in the interactions between plant and microbes,or among intraspecies or interspecies of microbes.Many mVOCs showed diverse agroactivities,such as insecticidal,bactericidal,fungicidal,herbicidal,plant growth promotion and abiotic stress-tolerance inducing activities.The agroactive mVOCs have diverse structure-types,such as alkane,alkenes,alcohols,aldehydes,ketones,carboxylic acid,esters and lactones,ethers,aromatic ring,terpenes,heterocycles,sulphur-containing VOCs.The promising agroactive properties make it possible to apply mVOCs and their producing microorganisms or synthetic mimics or agroactive mVOCs in crop protection,and so this review focuses on the chemical diversity,agroactivities,and potential application of mVOCs.展开更多
Despite its economic importance of tomato(Solamum lycopersicum L.),many growers are unable to achieve maximum yield of high quality due to insect pest infestation.This study was aimed at determining the effec-tiveness...Despite its economic importance of tomato(Solamum lycopersicum L.),many growers are unable to achieve maximum yield of high quality due to insect pest infestation.This study was aimed at determining the effec-tiveness of parasitoid Eretmocerus eremicus and crude plant extracts of garlic and chilli as an integrated alternative control method of greenhouse whitefly(GHW)on yield of tomato variety“Anna F1”The study was.conducted at Egerton University using a split plot arrangement in ran-domized complete block design,replicated three times.Parasitoid was the main plot factor at two levels(with and without E.eremicus)while sprays of distilled water(negative control),Abamectin(positive control),chilli and garlic extracts formed the sub-plot factors.Data on GHW incidence,population density,parasitisation rate,and yield was collected.Data collected was subjected to analysis of variance and means of significant treatments separated using Tukey's test at p=0.05.Botanical extracts and E.eremicus helped to control whitefly population,infestation,and in en-hancing growth and yield of greenhouse tomato plants compared to where they were not applied.Botanical plant extracts and/or E.eremicus can be used in the management of GHW and to improve tomato yields.展开更多
The frequent outbreaks of crop diseases pose a serious threat to global agricultural production and food security.Data-driven forecasting models have emerged as an effective approach to support early warning and manag...The frequent outbreaks of crop diseases pose a serious threat to global agricultural production and food security.Data-driven forecasting models have emerged as an effective approach to support early warning and management,yet the lack of user-friendly tools for model development remains a major bottleneck.This study presents the Multi-Scenario Crop Disease Forecasting Modeling System(MSDFS),an open-source platform that enables end-to-end model construction-from multi-source data ingestion and feature engineering to training,evaluation,and deployment-across four representative scenarios:static point-based,static grid-based,dynamic point-based,and dynamic grid-based.Unlike conventional frameworks,MSDFS emphasizes modeling flexibility,allowing users to build,compare,and interpret diverse forecasting approaches within a unified workflow.A notable feature of the system is the integration of a weather scenario generator,which facilitates comprehensive testing of model performance and adaptability under extreme climatic conditions.Case studies corresponding to the four scenarios were used to validate the system,with overall accuracy(OA)ranging from 73%to 93%.By lowering technical barriers,the system is designed to serve plant protection managers and agricultural producers without advanced programming expertise,providing a practical modeling tool that supports the construction of smart plant protection systems.展开更多
This study evaluated the efficiency of different soilless growth media for sustainable basil cultivation compared to traditional potting mix with continuous monitoring.This paper presents a novel approach of continuou...This study evaluated the efficiency of different soilless growth media for sustainable basil cultivation compared to traditional potting mix with continuous monitoring.This paper presents a novel approach of continuous physicochemical monitoring of basil growth using Internet of Things(IoT)enabled smart growth cabinets.Six growth media combinations-sand,coir,and biochar(unsoaked and nutrient-enriched),sand,coir,and perlite,and potting mix with 10%and 20%biochar-were tested over 30 days under controlled conditions,with potting mix as the control.The pH,electrical conductivity and cation exchange capacity of growth mixes were analyzed before and after,along with key growth metrics such as root length,shoot length,leaf number,fresh and dry plant weight and leaf area index(LAI)were analysed.Results indicated that incorporating 10 to 20%biochar into potting mix optimally enhanced basil growth,with significant improvements in root development and the LAI of the plant.Biochar soaked in nutrient solution demonstrated three times higher plant weight compared to unsoaked biochar,indicating the potential of biochar as a slow-release nutrient matrix.Despite the high exchangeable potassium and sodium of biochar,calcium and magnesium remained dominant in the potting mix,indicating the need for optimising biochar use as a horticultural growth media according to the plant type chosen.Replacement of 10 to 20%of potting mix by biochar supports the circular economy goals by enhancing plant growth and sequestering carbon.展开更多
RNA silencing(or RNA interference,RNAi)is a conserved mechanism for regulating gene expression in eukaryotes.The discovery of natural trans-kingdom RNAi indicated that small RNAs act as signaling molecules and enable ...RNA silencing(or RNA interference,RNAi)is a conserved mechanism for regulating gene expression in eukaryotes.The discovery of natural trans-kingdom RNAi indicated that small RNAs act as signaling molecules and enable communication between organisms in different kingdoms.The phenomenon and potential mechanisms of trans-kingdom RNAi are among the most exciting research topics.To better understand trans-kingdom RNAi,we review the history of the discovery and elucidation of RNAi mechanisms.Based on canonical RNAi mechanisms,we summarize the major points of divergence around RNAi pathways in the main eukaryotes’kingdoms,including plants,animals,and fungi.We review the representative incidents associated with the mechanisms and applications of trans-kingdom RNAi in crop protection,and discuss the critical factors that should be considered to develop successful trans-kingdom RNAi-based crop protection strategies.展开更多
Soil microbial mutualists of plants, including mycorrhizal ftmgi, non- mycorrhizal fungi and plant growth promoting rhizobacteria, have been typically characterized for increasing nutrient acquisition and plant growth...Soil microbial mutualists of plants, including mycorrhizal ftmgi, non- mycorrhizal fungi and plant growth promoting rhizobacteria, have been typically characterized for increasing nutrient acquisition and plant growth. More recently, soil microbes have also been shown to increase direct plant defense against above- and below- ground herbivores. Plants, however, do not only rely on direct defenses when attacked, but they can also recruit pest antagonists such as predators and parasitoids, both above and belowground, mainly via the release of volatile organic compounds （i.e., indirect defenses）. In this review, we illustrate the main features and effects of soil microbial mutualists of plants on plant indirect defenses and discuss possible applications within the framework of sustainable crop protection against root- and shoot-feeding arthropod pests. We indicate the main knowledge gaps and the future challenges to be addressed in the study and application of these rnultifaceted interactions.展开更多
Plants possess effective immune systems that defend against most microbial attackers.Recent plant immunity research has focused on the classic binary defense model involving the pivotal role of small-molecule hormones...Plants possess effective immune systems that defend against most microbial attackers.Recent plant immunity research has focused on the classic binary defense model involving the pivotal role of small-molecule hormones in regulating the plant defense signaling network.Although most of our current understanding comes from studies that relied on information derived from a limited number of pathosystems,newer studies concerning the incredibly diverse interactions between plants and microbes are providing additional insights into other novel mechanisms.Here,we review the roles of both classical and more recently identified components of defense signaling pathways and stress hormones in regulating the ambivalence effect during responses to diverse pathogens.Because of their different lifestyles,effective defense against biotrophic pathogens normally leads to increased susceptibility to necrotrophs,and vice versa.Given these opposing forces,the plant potentially faces a trade-off when it mounts resistance to a specific pathogen,a phenomenon referred to here as the ambivalence effect.We also highlight a novel mechanism by which translational control of the proteins involved in the ambivalence effect can be used to engineer durable and broad-spectrum disease resistance,regardless of the lifestyle of the invading pathogen.展开更多
基金supported by the National Natural Science Foundation of China(22071072,21801086)the Fundamental Research Funds for the Central Universities of China(2662022LXYJ001)。
文摘Melatonin(N-Acetyl-5-methoxytryptamine),an endogenously synthesized indoleamine,exerts pleiotropic effects in plant physiology by interacting with other phytohormones,thereby synergistically regulating plant growth,development,and stress responses.Recent research has also indicated that melatonin derivatives could be further developed as promising antifungal candidates.Given the significant roles of melatonin,considerable efforts have been devoted to studying its potent functions and underlying mechanisms.This review outlines recent advancements in understanding the roles of melatonin in regulating plant growth and its potential synergistic interactions with pathogens.Additionally,we present our perspectives aimed at elucidating the antifungal mechanisms of melatonin and its derivatives,which could facilitate the development of naturally sourced fungicides and offer innovative strategies for pesticide discovery.
基金supported by the National Natural Science Foundation of China(No.31950410547 and 42107270).
文摘Ozone(O_(3))pollution has elevated in China,threatening plants and crop production.Ethylenediurea(EDU)is a chemical alleviating O_(3)-induced phytotoxicities.This study aimed at revealing fluctuating O_(3) exposures effects on Youxian No 3(Phaseolus vulgaris)and Sukui No 4(Vigna angularis),two widely grown Chinese bean cultivars,and EDU role in mediating these effects.Plants were periodically treated with EDU(400 mg/L)or water and subjected to charcoal-filtered air(CF)or non-filtered ambient air enriched with an additional targeted O_(3) concentration of 40 ppb(NF40).with subsequent ambient or NF40 exposures.A 10-day exposure to NF40 increased photosynthetic rate(A)while decreasing the leaf intercellular CO_(2) concentration(Ci),but this effect was absent after moving plants to ambient air for two weeks.Moving previously CF-exposed plants to ambient air for two weeks also increased A,which was not linked with Ci but more related to stomatal conductance(gs).Following two one-week and two-week sequential exposures of all plants to NF40,with an intermediate exposure to ambient air,elevated O_(3) reduced chlorophylls(SPAD),A,gs,Ci,and transpiration and decoupled A-gs response.More O_(3) effectswere observed in plants treatedwithNF40 during each O_(3)-treatment cycle,compared to those exposed to CF during the first cycle.The former plants exhibited significantly decreased biomass and water content of leaves and stems but increased flowers biomass and water content.Some of the effects were cultivardependent,with Youxian showing more apparent trade-offs between vegetative growth and reproduction.EDU alleviated various negative O_(3) effects.
基金funded by the National Natural Science Foundation of China(32188102 to Lanqin Xia)Natural Science Foundation for Distinguished Young Scholars of Jiangxi province(20212ACB215001 to Xiudao Yu)+1 种基金supported by the China Scholarship Council(202303250062)the GSCAAS-ULg Joint PhD Program。
文摘RNA interference(RNAi)has been used for agricultural insect pest control based on silencing of targeted insect genes.However,the effectiveness of RNAi and its applications in insect pest control remain challenging.Here we review factors that may affect the effectiveness of RNAi application,including the variability in RNAi efficacy among different insect species,a limited understanding of double-stranded RNA(dsRNA)uptake and systemic RNAi mechanisms,and the effective delivery of dsRNA in field conditions.Furthermore,we summarize recent progress in RNAi strategies for crop protection,discuss the advantages and disadvantages of RNAi-based insect control,and propose potential strategies to increase the effectiveness of RNAi in insect control.
基金supported by grants from the National Key Research and Development Program(2023YFE0113600).
文摘RNA interference(RNAi)is a post-transcriptional gene-silencing technique induced by the introduction of double-stranded RNA(dsRNA)or small interfering RNA(siRNA)[1].RNAi-based strategies have been extensively applied in the treatment of human diseases and crop protection against insect pests[2-4].With the availability of the full genome sequences of major mosquito vectors,RNAi has become increasingly used as a novel means of mosquito control[5].
基金the AgriFose2030 programmethe swedish International Development Cooperation Agency (SIDA)the Swedish Foundation for International Cooperation in Research and Higher Education (STINT) for financial support
文摘This review provides a summary of botanicals and plant strengtheners that have potential uses for disease and pest management in potato and tomato cultivation in African.We discuss their possible use to prevent major diseases and pests which infest potato and tomato,such as early and late blight,bacterial wilt,potato tuber moth,and tomato leafminer.There are several examples of the successful uses of botanicals for pathogen and pest control relevant for different African climatic conditions;however,most of these studies have been conducted in vitro and often lack field verification.Plant strengtheners(substances that induce and improve crop resistance,yield,and quality)are little studied and used in Africa in comparison to North America and Europe.The possible benefits of using botanicals and plant strengtheners instead of conventional pesticides are discussed here in relation to human health and the environment as well as their modes of action and accessibility to farmers.Lack of knowledge of the composition and active ingredients of extracts,environmental concerns,uncertainties regarding stability and formulation,lack of legislation and limited support from governments,hamper the development of botanicals and plant strengtheners for use in sustainable African agriculture.
基金supported by grants from the Major Program of National Agricultural Science and Technology of China(NK20220607)the National Natural Science Foundation of China(32272059 and 31971883)the Science and Technology Department of Sichuan Province(2021YFYZ0002,2022ZDZX0014,and 2023NSFSC1995)。
文摘Stripe rust,caused by Puccinia striiformis f.sp.tritici(Pst),is a devastating disease in wheat worldwide.Discovering and characterizing new resistance genes/QTL is crucial for wheat breeding programs.In this study,we fine-mapped and characterized a stripe rust resistance gene,YRAYH,on chromosome arm 5BL in the Chinese wheat landrace Anyuehong(AYH).Evaluations of stripe rust response to prevalent Chinese Pst races in near-isogenic lines derived from a cross of Anyuehong and Taichung 29 showed that YrAYH conferred a high level of resistance at all growth stages.Fine mapping using a large segregating population of 9748 plants,narrowed the YRAYH locus to a 3.7 Mb interval on chromosome arm 5BL that included 61 annotated genes.Transcriptome analysis of two NIL pairs identified 64 upregulated differentially expressed genes(DEGs)in the resistant NILs(NILs-R).Annotations indicated that many of these genes have roles in plant disease resistance pathways.Through a combined approach of fine-mapping and transcriptome sequencing,we identified a serine/threonine-protein kinase SRPK as a candidate gene underlying YrAYH.A unique 25 bp insertion was identified in the NILs-R compared to the NILs-S and previously published wheat genomes.An InDel marker was developed and co-segregated with YrAYH.Agronomic trait evaluation of the NILs suggested that YrAYH not only reduces the impact of stripe rust but was also associated with a gene that increases plant height and spike length.
文摘The safety of crop protection products and their safe use is of paramount importance and requires that crop protection products are consistently marketed and used worldwide in the required registered quality.To meet these high-quality requirements,the highest demands must be placed on the production and purification of technical-grade crop protection products.In this context,impurity profiles are of utmost importance,as unknown impurities at higher than registered concentrations may pose new unknown toxicological risks to users,consumers,and the environment.Particularly high demands are placed on the production and purification of technical grade semisynthetic derivatives of fermentatively derived natural products to meet the required regulatory specifications,to ensure that relevant impurities do not exceed maximum allowable concentrations,to ensure the safe use of such products,and to eliminate any unknown product safety risks.The challenges associated with the manufacture and purification of Afidopyropen and the product safety risks associated with various qualities of technical grade Emamectin benzoate are discussed in detail.
文摘Birds are a huge hazard to agriculture all around the world,causing harm to profitable field crops.Growers use a variety of techniques to keep them away,including visual,auditory,tactile,and olfactory deterrents. This study presents a comprehensive overview of current bird repellant approaches used in agricultural contexts,as well as potential new ways. The bird repellent techniques include Internet of Things technology,Deep Learning,Convolutional Neural Network,Unmanned Aerial Vehicles,Wireless Sensor Networks and Laser biotechnology. This study’s goal is to find and review about previous approach towards repellent of birds in the crop fields using various technologies.
基金Supported by the Foundation for the Excellent Academic Pacemakers in Harbin City,Heilongjiang Province(2013RFXXJ033)Natural Science Foundation of Heilongjiang Province,China(E201249)
文摘The increasing of highway engineering construction makes soil and water loss surrounding highway and also influences the growth of crops around, To control soil and water loss, new engineering measures are put forward to protect soil and water and prevent crops from being damaged.
文摘Crop pests are a permanent threat to horticultural production. Several control methods are recommended for their control, including biological control. This method based on the use of natural enemies is not well established. The objective of this study was to assess the diversity and abundance of natural enemies of crop pests. A survey of crops associated with pests was carried out on 144 fields in southern Senegal to measure the diversity and abundance of natural enemies of various crop pests, determine the native parasitoid complex and assess parasitism rates. Ecological indices were calculated to assess the diversity of natural enemies. A total of 25 natural enemy species were identified, including 15 parasitoids and 10 predators. Predatory Hemiptera and Parasitoid Hymenoptera were high biodiversity. The parasitism rates vary between 0 and 50% depending on the abundance of pests and parasitoid species. Knowledge of the diversity of natural enemies and the understanding of trophic interactions with pests are important in the development of biological crop protection in order to preserve resilience in agroecosystems.
文摘World crop production requires highly-productive varieties of agricultural crops,which are resistant to pest organisms.Such varieties are also of great importance for the Uzbekistan.Their
文摘The introduction of invasive insect pests across national borders has become a major concern in crop production. Accordingly, national plant protection organizations are challenge to reinforce their monitoring strategies, which are hampered by the weight and size of inspection equipment, as well as the taxonomic extensiveness of interrupted species. Moreover, some insect pests that impede farmer productivity and profitability are difficult for researchers to address on time due to a lack of appropriate plant protection measures. Farmers’ reliance on synthetic pesticides and biocontrol agents has resulted in major economic and environmental ramifications. DNA barcoding is a novel technology that has the potential to improve Integrated Pest Management decision-making, which is dependent on the ability to correctly identify pest and beneficial organisms. This is due to some natural traits such as phenology or pesticide susceptibility browbeaten by IPM strategies to avert pest establishment. Specifically, Deoxyribonucleic acid (DNA) sequence information was applied effectively for the identification of some micro-organisms. This technology, DNA barcoding, allows for the identification of insect species by using short, standardized gene sequences. DNA barcoding is basically based on repeatable and accessible technique that allows for the mechanisation or automation of species discrimination. This technique bridges the taxonomic bio-security gap and meets the International Plant Protection Convention diagnostic standards for insect identification. This review therefore discusses DNA barcoding as a technique for insect pests’ identification and its potential application for crop protection.
基金the Foundation of Hubei Hongshan Labororaty(No.2021hskf016)Hubei Agricultural Science and Technology Innovation Center(2021-620-000-001-027)for financial support for this research.
文摘Synthetic chemicals have been widely used in modern agriculture as fertilizer,plant growth regulators and pesticides.Although the chemical inputs improved the crop production,it is well known about their negative effects,such as environmental residues in water,soil and animals,pest resistance and resurgence and the residues in agricultural produces.The demands for safer food and more friendly environment are now more preferred by humankind in the world.Microbes have already become an alternate for chemical inputs in some aspects in agriculture.The microbes can produce very diverse volatile organic compounds(mVOCs)which play important roles in the interactions between plant and microbes,or among intraspecies or interspecies of microbes.Many mVOCs showed diverse agroactivities,such as insecticidal,bactericidal,fungicidal,herbicidal,plant growth promotion and abiotic stress-tolerance inducing activities.The agroactive mVOCs have diverse structure-types,such as alkane,alkenes,alcohols,aldehydes,ketones,carboxylic acid,esters and lactones,ethers,aromatic ring,terpenes,heterocycles,sulphur-containing VOCs.The promising agroactive properties make it possible to apply mVOCs and their producing microorganisms or synthetic mimics or agroactive mVOCs in crop protection,and so this review focuses on the chemical diversity,agroactivities,and potential application of mVOCs.
文摘Despite its economic importance of tomato(Solamum lycopersicum L.),many growers are unable to achieve maximum yield of high quality due to insect pest infestation.This study was aimed at determining the effec-tiveness of parasitoid Eretmocerus eremicus and crude plant extracts of garlic and chilli as an integrated alternative control method of greenhouse whitefly(GHW)on yield of tomato variety“Anna F1”The study was.conducted at Egerton University using a split plot arrangement in ran-domized complete block design,replicated three times.Parasitoid was the main plot factor at two levels(with and without E.eremicus)while sprays of distilled water(negative control),Abamectin(positive control),chilli and garlic extracts formed the sub-plot factors.Data on GHW incidence,population density,parasitisation rate,and yield was collected.Data collected was subjected to analysis of variance and means of significant treatments separated using Tukey's test at p=0.05.Botanical extracts and E.eremicus helped to control whitefly population,infestation,and in en-hancing growth and yield of greenhouse tomato plants compared to where they were not applied.Botanical plant extracts and/or E.eremicus can be used in the management of GHW and to improve tomato yields.
基金supported by Zhejiang Provincial Natural Science Foundation of China(Grant No.LR25D010003)The Zhejiang Provincial Key Research and Development Program(Grant No.2023C02018)National Natural Science Foundation of China(Grant No.42401400).
文摘The frequent outbreaks of crop diseases pose a serious threat to global agricultural production and food security.Data-driven forecasting models have emerged as an effective approach to support early warning and management,yet the lack of user-friendly tools for model development remains a major bottleneck.This study presents the Multi-Scenario Crop Disease Forecasting Modeling System(MSDFS),an open-source platform that enables end-to-end model construction-from multi-source data ingestion and feature engineering to training,evaluation,and deployment-across four representative scenarios:static point-based,static grid-based,dynamic point-based,and dynamic grid-based.Unlike conventional frameworks,MSDFS emphasizes modeling flexibility,allowing users to build,compare,and interpret diverse forecasting approaches within a unified workflow.A notable feature of the system is the integration of a weather scenario generator,which facilitates comprehensive testing of model performance and adaptability under extreme climatic conditions.Case studies corresponding to the four scenarios were used to validate the system,with overall accuracy(OA)ranging from 73%to 93%.By lowering technical barriers,the system is designed to serve plant protection managers and agricultural producers without advanced programming expertise,providing a practical modeling tool that supports the construction of smart plant protection systems.
基金supported by a Seed Grant from Centre of Sustainable Bioproducts,Deakin University.
文摘This study evaluated the efficiency of different soilless growth media for sustainable basil cultivation compared to traditional potting mix with continuous monitoring.This paper presents a novel approach of continuous physicochemical monitoring of basil growth using Internet of Things(IoT)enabled smart growth cabinets.Six growth media combinations-sand,coir,and biochar(unsoaked and nutrient-enriched),sand,coir,and perlite,and potting mix with 10%and 20%biochar-were tested over 30 days under controlled conditions,with potting mix as the control.The pH,electrical conductivity and cation exchange capacity of growth mixes were analyzed before and after,along with key growth metrics such as root length,shoot length,leaf number,fresh and dry plant weight and leaf area index(LAI)were analysed.Results indicated that incorporating 10 to 20%biochar into potting mix optimally enhanced basil growth,with significant improvements in root development and the LAI of the plant.Biochar soaked in nutrient solution demonstrated three times higher plant weight compared to unsoaked biochar,indicating the potential of biochar as a slow-release nutrient matrix.Despite the high exchangeable potassium and sodium of biochar,calcium and magnesium remained dominant in the potting mix,indicating the need for optimising biochar use as a horticultural growth media according to the plant type chosen.Replacement of 10 to 20%of potting mix by biochar supports the circular economy goals by enhancing plant growth and sequestering carbon.
基金supported by the National Natural Science Foundation of China(32020103003,31730078,and 32170293)。
文摘RNA silencing(or RNA interference,RNAi)is a conserved mechanism for regulating gene expression in eukaryotes.The discovery of natural trans-kingdom RNAi indicated that small RNAs act as signaling molecules and enable communication between organisms in different kingdoms.The phenomenon and potential mechanisms of trans-kingdom RNAi are among the most exciting research topics.To better understand trans-kingdom RNAi,we review the history of the discovery and elucidation of RNAi mechanisms.Based on canonical RNAi mechanisms,we summarize the major points of divergence around RNAi pathways in the main eukaryotes’kingdoms,including plants,animals,and fungi.We review the representative incidents associated with the mechanisms and applications of trans-kingdom RNAi in crop protection,and discuss the critical factors that should be considered to develop successful trans-kingdom RNAi-based crop protection strategies.
文摘Soil microbial mutualists of plants, including mycorrhizal ftmgi, non- mycorrhizal fungi and plant growth promoting rhizobacteria, have been typically characterized for increasing nutrient acquisition and plant growth. More recently, soil microbes have also been shown to increase direct plant defense against above- and below- ground herbivores. Plants, however, do not only rely on direct defenses when attacked, but they can also recruit pest antagonists such as predators and parasitoids, both above and belowground, mainly via the release of volatile organic compounds （i.e., indirect defenses）. In this review, we illustrate the main features and effects of soil microbial mutualists of plants on plant indirect defenses and discuss possible applications within the framework of sustainable crop protection against root- and shoot-feeding arthropod pests. We indicate the main knowledge gaps and the future challenges to be addressed in the study and application of these rnultifaceted interactions.
基金supported by grants from the National Research Foundation of Korea(NRF)(2018R1A5A1023599,2020R1A2B5B03096402,and 2021M3H9A1096935 to Y.-H.L.and 2019R1I1A1A01059802 to C.-Y.K.).C.-Y.K.is grateful for a graduate fellowship from the Brain Korea 21 Plus Program.
文摘Plants possess effective immune systems that defend against most microbial attackers.Recent plant immunity research has focused on the classic binary defense model involving the pivotal role of small-molecule hormones in regulating the plant defense signaling network.Although most of our current understanding comes from studies that relied on information derived from a limited number of pathosystems,newer studies concerning the incredibly diverse interactions between plants and microbes are providing additional insights into other novel mechanisms.Here,we review the roles of both classical and more recently identified components of defense signaling pathways and stress hormones in regulating the ambivalence effect during responses to diverse pathogens.Because of their different lifestyles,effective defense against biotrophic pathogens normally leads to increased susceptibility to necrotrophs,and vice versa.Given these opposing forces,the plant potentially faces a trade-off when it mounts resistance to a specific pathogen,a phenomenon referred to here as the ambivalence effect.We also highlight a novel mechanism by which translational control of the proteins involved in the ambivalence effect can be used to engineer durable and broad-spectrum disease resistance,regardless of the lifestyle of the invading pathogen.
