Due to the rapid development of obesity and related metabolic diseases in the world,it is particularly important to explore the ability of novel materials in regulating obesity.In this study,C57BL/6J obese mice were u...Due to the rapid development of obesity and related metabolic diseases in the world,it is particularly important to explore the ability of novel materials in regulating obesity.In this study,C57BL/6J obese mice were used as objects,and 16S rRNA gene sequencing and lipidomics techniques were used to explore the regulatory effect and mechanism of the Protaetia brevitarsis ethanol extract(50%,V/V)against obesity.Our r esults showed that P.brevitarsis extract could significantly reduce the body weight,triglyceride(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),and pro-inflammatory cytokines(tumor necrosis factor-α(TNF-α),interferon-γ(IFN-γ),and interleukin-6(IL-6))levels of high-fat diet(HFD)-fed mice.Moreover,the P.brevitarsis extract changed the composition and relative abundance of its gut microbiota,especially increasing Akkermansia muciniphila.Meanwhile,the content of short-chain fatty acids(SCFAs)was increased,especially acetic acid and butyric acid,which leads to a decrease in the synthesis and accumulation of lipids in the liver.Furthermore,the P.brevitarsis extract also induced the difference in lipid synthesis in the liver by down-regulating the mRNA levels of sterol regulatory element binding protein-1C(SREBP-1C),fatty acid synthase(FAS),and acetyl-CoA carboxylase(ACC).Therefore,this research provided preliminary work and reference for the development of P.brevitarsis as a functional food for anti-obesity.展开更多
Objective:To investigate the effect of an aqueous extract of Protaetia brevitarsis(AEPB)on osteogenesis using preosteoblast MC3T3-E1 cells and zebrafish larvae.Methods:Flow cytometric analysis was used to measure the ...Objective:To investigate the effect of an aqueous extract of Protaetia brevitarsis(AEPB)on osteogenesis using preosteoblast MC3T3-E1 cells and zebrafish larvae.Methods:Flow cytometric analysis was used to measure the cytotoxicy.Alkaline phosphatase activity was detetmined using p-nitrophenyl phosphate as a substrate.Calcium deposition was detected using alizarin red staining along with osteogenic marker expression in preosteoblast MC3T3E1 cells.In addition,vertebral formation in zebrafish larvae was detected using calcein staining and osteogenic gene expression.Results:AEPB highly promoted the expression of osteogenic markers including runt-related transcription factor 2,osterix,and alkaline phosphatase,along with elevated levels of mineralization in MC3T3-E1 cells.Moreover,AEPB accelerated vertebral formation in zebrafish larvae accompanied by upregulated expression of osteogenic genes.FH535,an inhibitor of Wnt/β-catenin,suppressed AEPB-induced osteogenic gene expression and vertebral formation,indicating that AEPB stimulates osteogenesis by activating the Wnt/β-catenin signaling pathway.Conclusions:AEPB stimulates osteoblast differentiation and bone formation by activatingβ-catenin.Therefore,AEPB is a promising material that induces osteogenesis,and is useful for the treatment of bone resorption diseases.展开更多
Objective:To evaluate the effects of an aqueous extract of Protaetia brevitarsis(AEPB)on the growth of zebrafish and preosteoblast MC3T3-E1 cells.Methods:The effects of AEPB on the linear growth and the expression of ...Objective:To evaluate the effects of an aqueous extract of Protaetia brevitarsis(AEPB)on the growth of zebrafish and preosteoblast MC3T3-E1 cells.Methods:The effects of AEPB on the linear growth and the expression of growth-related genes in zebrafish and MC3T3-E1 cells were assessed using various molecular techniques.Furthermore,the involvement of the mammalian target of rapamycin(mTOR)pathway in AEPB-induced growth was investigated by employing the mTOR inhibitor rapamycin.Results:AEPB administration led to a significant and dose-dependent increase in zebrafish larvae growth over time.Additionally,AEPB treatment upregulated the expression of growth hormone-1(GH-1),insulin-like growth factor-1(IGF-1),growth hormone receptor-1(GHR-1),and cholecystokinin-a(CCKA)in zebrafish.Similarly,AEPB stimulated the expression and release of IGF-1 and accelerated mTOR expression in MC3T3-E1 cells.In addition,rapamycin hindered AEPB-induced linear growth in zebrafish larvae and suppressed the expression of growth-promoting genes by inhibiting mTOR activation.Conclusions:AEPB shows growth-promoting effects by upregulating growth-related genes and activating the mTOR signaling pathway.Further investigations are warranted to elucidate its mechanisms of action and explore its potential application in the development of growth-enhancing supplements for various purposes.展开更多
Edible insects have been attracting significant attention not only for their nutritional superiority but also for their excellent bio-functional activity.In this study,the apoptotic protective effects of water-soluble...Edible insects have been attracting significant attention not only for their nutritional superiority but also for their excellent bio-functional activity.In this study,the apoptotic protective effects of water-soluble protein(WPB)and salt-soluble protein(SPB)extracts derived from Protaetia brevitarsis against hydrogen peroxide(H_(2)O_(2))-induced oxidative stress in C2C12 cells were investigated.WPB and SPB were confirmed to exhibit radical scavenging and ferric-ion reducing activities.In addition,WPB and SPB treatments effectively restored the decreased cell viability of the C2C12 cells induced by H2O2 treatment,and showcased protective effects against apoptosis and mitochondrial dysfunction by regulating the expressions of pro-and anti-apoptotic proteins.Therefore,our data indicated that protein extracts from Protaetia brevitarsis could be beneficial for the prevention and treatment of diseases associated with apoptosis induced by oxidative stress.展开更多
To improve the survival rate of larvae during material separation after biotransformation of existing residual film mixtures of Protaetia brevitarsis larvae,this paper adopts the method of combining physical test and ...To improve the survival rate of larvae during material separation after biotransformation of existing residual film mixtures of Protaetia brevitarsis larvae,this paper adopts the method of combining physical test and EDEM simulation test,and selects Hertz Mindlin with JKR contact model to calibrate the discrete element simulation contact parameters of the Protaetia brevitarsis larvae and the frass mixture.First,the cylinder lifting method was used to determine the actual repose angle of the mixture of larvae and frass.The collision recovery coefficients between larvae-frass and steel,static friction coefficient,kinetic friction coefficient and the collision recovery coefficient between larvae were measured through physical tests such as the inclined plane method.The Plackett-Burman test was then used to screen out the factors that have a significant impact on the repose angle:Poisson’s ratio of frass,frass-frass rolling friction coefficient,frass JKR surface energy,frass-larvae JKR surface energy.The optimal value intervals of four significant factors were determined based on the steepest climb test,Based on the Box-Behnken response surface analysis test,the second-order regression model between the repose angle and four significant factors was determined,and variance and interaction effects were analyzed.And with the actual repose angle as the goal,the significant factors were optimized and the optimal parameter combination of the four significant factors was determined.The simulation test of material repose angle and screening was carried out with the optimal parameter combination,and compared with the physical test.It was found that the maximum relative errors of the two tests were 1.48%and 3.79%respectively,indicating that the calibrated parameter values are true and reliable,It can provide a reference for the discrete element simulation of the transportation and separation of the Protaetia brevitarsis larvae-frass mixture.展开更多
为了从白星花金龟幼虫虫粪中分离筛选具有除氨效果的微生物,为有机废弃物转化利用提供微生物资源。采用富集驯化、初筛、复筛相结合的方式从白星花金龟幼虫虫粪中筛选出2株除氨菌种N1、N2,结合形态、生理生化、16S r DNA分子鉴定菌株类...为了从白星花金龟幼虫虫粪中分离筛选具有除氨效果的微生物,为有机废弃物转化利用提供微生物资源。采用富集驯化、初筛、复筛相结合的方式从白星花金龟幼虫虫粪中筛选出2株除氨菌种N1、N2,结合形态、生理生化、16S r DNA分子鉴定菌株类别,对菌株的生理性状和生长曲线进行测定;并通过硼酸吸收凯氏定氮法测定单菌株和复配菌株在25 d内对NH_(3)释放的影响。结果表明:N1和N2均表现出了明显的除氨能力,复配菌株的除氨效果高于单菌株。经鉴定N1为小麦苍白杆菌,N2为苍白杆菌属。N1和N2生长曲线各具特点。两菌株在30~37℃pH=5~9时生长情况最好。从白星花金龟幼虫虫粪中分离的2株菌株能有效降低NH3释放量,2株菌株复配的除氨效果高于单菌株,可作为有机废弃物在发酵腐熟期间有效的除氨微生物。展开更多
基金finally supported by the National Natural Science Foundation of China(31830084,31970440)the construction funds for the‘Double First-Class’initiative for Nankai University(96172158,96173250,91822294)。
文摘Due to the rapid development of obesity and related metabolic diseases in the world,it is particularly important to explore the ability of novel materials in regulating obesity.In this study,C57BL/6J obese mice were used as objects,and 16S rRNA gene sequencing and lipidomics techniques were used to explore the regulatory effect and mechanism of the Protaetia brevitarsis ethanol extract(50%,V/V)against obesity.Our r esults showed that P.brevitarsis extract could significantly reduce the body weight,triglyceride(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),and pro-inflammatory cytokines(tumor necrosis factor-α(TNF-α),interferon-γ(IFN-γ),and interleukin-6(IL-6))levels of high-fat diet(HFD)-fed mice.Moreover,the P.brevitarsis extract changed the composition and relative abundance of its gut microbiota,especially increasing Akkermansia muciniphila.Meanwhile,the content of short-chain fatty acids(SCFAs)was increased,especially acetic acid and butyric acid,which leads to a decrease in the synthesis and accumulation of lipids in the liver.Furthermore,the P.brevitarsis extract also induced the difference in lipid synthesis in the liver by down-regulating the mRNA levels of sterol regulatory element binding protein-1C(SREBP-1C),fatty acid synthase(FAS),and acetyl-CoA carboxylase(ACC).Therefore,this research provided preliminary work and reference for the development of P.brevitarsis as a functional food for anti-obesity.
基金Korea Environment Industry&Technology Institute through Project to Make Multi-ministerial National Biological Research Resources more Advanced funded by Korea Ministry of Environment(No.:1485018221)Basic Science Research Program to Research Institute for Basic Sciences of Jeju National University through the National Research Foundation of Korea funded by the Ministry of Education(2019R1A6A1A10072987).
文摘Objective:To investigate the effect of an aqueous extract of Protaetia brevitarsis(AEPB)on osteogenesis using preosteoblast MC3T3-E1 cells and zebrafish larvae.Methods:Flow cytometric analysis was used to measure the cytotoxicy.Alkaline phosphatase activity was detetmined using p-nitrophenyl phosphate as a substrate.Calcium deposition was detected using alizarin red staining along with osteogenic marker expression in preosteoblast MC3T3E1 cells.In addition,vertebral formation in zebrafish larvae was detected using calcein staining and osteogenic gene expression.Results:AEPB highly promoted the expression of osteogenic markers including runt-related transcription factor 2,osterix,and alkaline phosphatase,along with elevated levels of mineralization in MC3T3-E1 cells.Moreover,AEPB accelerated vertebral formation in zebrafish larvae accompanied by upregulated expression of osteogenic genes.FH535,an inhibitor of Wnt/β-catenin,suppressed AEPB-induced osteogenic gene expression and vertebral formation,indicating that AEPB stimulates osteogenesis by activating the Wnt/β-catenin signaling pathway.Conclusions:AEPB stimulates osteoblast differentiation and bone formation by activatingβ-catenin.Therefore,AEPB is a promising material that induces osteogenesis,and is useful for the treatment of bone resorption diseases.
基金supported by the 2023 scientific promotion program funded by Jeju National University.
文摘Objective:To evaluate the effects of an aqueous extract of Protaetia brevitarsis(AEPB)on the growth of zebrafish and preosteoblast MC3T3-E1 cells.Methods:The effects of AEPB on the linear growth and the expression of growth-related genes in zebrafish and MC3T3-E1 cells were assessed using various molecular techniques.Furthermore,the involvement of the mammalian target of rapamycin(mTOR)pathway in AEPB-induced growth was investigated by employing the mTOR inhibitor rapamycin.Results:AEPB administration led to a significant and dose-dependent increase in zebrafish larvae growth over time.Additionally,AEPB treatment upregulated the expression of growth hormone-1(GH-1),insulin-like growth factor-1(IGF-1),growth hormone receptor-1(GHR-1),and cholecystokinin-a(CCKA)in zebrafish.Similarly,AEPB stimulated the expression and release of IGF-1 and accelerated mTOR expression in MC3T3-E1 cells.In addition,rapamycin hindered AEPB-induced linear growth in zebrafish larvae and suppressed the expression of growth-promoting genes by inhibiting mTOR activation.Conclusions:AEPB shows growth-promoting effects by upregulating growth-related genes and activating the mTOR signaling pathway.Further investigations are warranted to elucidate its mechanisms of action and explore its potential application in the development of growth-enhancing supplements for various purposes.
基金This research was supported by the Main Research Program(E0211200-03)of the Korea Food Research Institute(KFRI)funded by the Ministry of Science and ICT(Republic of Korea).
文摘Edible insects have been attracting significant attention not only for their nutritional superiority but also for their excellent bio-functional activity.In this study,the apoptotic protective effects of water-soluble protein(WPB)and salt-soluble protein(SPB)extracts derived from Protaetia brevitarsis against hydrogen peroxide(H_(2)O_(2))-induced oxidative stress in C2C12 cells were investigated.WPB and SPB were confirmed to exhibit radical scavenging and ferric-ion reducing activities.In addition,WPB and SPB treatments effectively restored the decreased cell viability of the C2C12 cells induced by H2O2 treatment,and showcased protective effects against apoptosis and mitochondrial dysfunction by regulating the expressions of pro-and anti-apoptotic proteins.Therefore,our data indicated that protein extracts from Protaetia brevitarsis could be beneficial for the prevention and treatment of diseases associated with apoptosis induced by oxidative stress.
基金supported by the Autonomous Region Key R&D Program of Xinjiang,China(Grant No.2022B02046).
文摘To improve the survival rate of larvae during material separation after biotransformation of existing residual film mixtures of Protaetia brevitarsis larvae,this paper adopts the method of combining physical test and EDEM simulation test,and selects Hertz Mindlin with JKR contact model to calibrate the discrete element simulation contact parameters of the Protaetia brevitarsis larvae and the frass mixture.First,the cylinder lifting method was used to determine the actual repose angle of the mixture of larvae and frass.The collision recovery coefficients between larvae-frass and steel,static friction coefficient,kinetic friction coefficient and the collision recovery coefficient between larvae were measured through physical tests such as the inclined plane method.The Plackett-Burman test was then used to screen out the factors that have a significant impact on the repose angle:Poisson’s ratio of frass,frass-frass rolling friction coefficient,frass JKR surface energy,frass-larvae JKR surface energy.The optimal value intervals of four significant factors were determined based on the steepest climb test,Based on the Box-Behnken response surface analysis test,the second-order regression model between the repose angle and four significant factors was determined,and variance and interaction effects were analyzed.And with the actual repose angle as the goal,the significant factors were optimized and the optimal parameter combination of the four significant factors was determined.The simulation test of material repose angle and screening was carried out with the optimal parameter combination,and compared with the physical test.It was found that the maximum relative errors of the two tests were 1.48%and 3.79%respectively,indicating that the calibrated parameter values are true and reliable,It can provide a reference for the discrete element simulation of the transportation and separation of the Protaetia brevitarsis larvae-frass mixture.
文摘为了从白星花金龟幼虫虫粪中分离筛选具有除氨效果的微生物,为有机废弃物转化利用提供微生物资源。采用富集驯化、初筛、复筛相结合的方式从白星花金龟幼虫虫粪中筛选出2株除氨菌种N1、N2,结合形态、生理生化、16S r DNA分子鉴定菌株类别,对菌株的生理性状和生长曲线进行测定;并通过硼酸吸收凯氏定氮法测定单菌株和复配菌株在25 d内对NH_(3)释放的影响。结果表明:N1和N2均表现出了明显的除氨能力,复配菌株的除氨效果高于单菌株。经鉴定N1为小麦苍白杆菌,N2为苍白杆菌属。N1和N2生长曲线各具特点。两菌株在30~37℃pH=5~9时生长情况最好。从白星花金龟幼虫虫粪中分离的2株菌株能有效降低NH3释放量,2株菌株复配的除氨效果高于单菌株,可作为有机废弃物在发酵腐熟期间有效的除氨微生物。
