Background： Sevoflurane and propofol are widely nsed anesthetics focused on changes in protein expression properties and membrane for surgery. Studies on the mechanisms of general anesthesia have lipid. MicroRNAs （m...Background： Sevoflurane and propofol are widely nsed anesthetics focused on changes in protein expression properties and membrane for surgery. Studies on the mechanisms of general anesthesia have lipid. MicroRNAs （miRNAs） regulate neural function by altering protein expression. We hypothesize that sevoflurane and propofol affect miRNA expression profiles in the brain, expect to understand the mechanism of anesthetic agents. Methods： Rats were randomly assigned to a 2% sevoflurane group, 600 μg-kg -1min propofol group, and a control group without anesthesia （n = 4, respectively）. Treatment group was under anesthesia for 6 h, and all rats breathed spontaneously with continuous monitoring of respiration and blood gases. Changes in rat cortex miRNA expression profiles were analyzed by miRNA microarrays and validated by quantitative real-time polymerase chain reaction （qRT-PCR）. Differential expression of miRNA using qRT-PCR among the control, sevoflurane, and propotbl groups were compared using one-way analysis of variance （ANOVA）. Results： Of 677 preloaded rat miRNAs, the microarray detected the expression of 277 miRNAs in rat cortex （40.9%）, of which 9 were regulated by propofol and （or） sevoflurane. Expression levels of three miRNAs （rno-miR-339-3p, rno-miR-448, rno-miR-466b- 1 ＊） were significantly increased following sevoflurane and six （rno-miR-339-3p, rno-miR-347, rno-miR-378＊, rno-miR-412＊, rno-miR-702-3p, and rno-miR-7a-2＊） following propofol. Three miRNAs （rno-miR-466b-I ＊, rno-miR-3584-Sp and rno-miR-702-3p） were differentially expressed by the two anesthetic treatment groups. Conclusions： Sevoflurane and propofol anesthesia induced distinct changes in brain miRNA expression patterns, suggesting differential regulation of protein expression. Determining the targets of these differentially expressed miRNAs may help reveal both the common and agent-specific actions of anesthetics on neurological and physiological function.展开更多
Objective： To investigate the influence of electroacupuncture on auditory evoked potential index （AAI） during propofol sedation. Methods： According to propofol effect site concentration, 24 patients for operation ...Objective： To investigate the influence of electroacupuncture on auditory evoked potential index （AAI） during propofol sedation. Methods： According to propofol effect site concentration, 24 patients for operation were randomly allocated to group 1 （1.0 μg/mL）, group 2 （1. 5μg/mL） and group 3 （2.0 μg/mL）. Propofol was administered intravenously, points Hegu （LI4） and Neiguan （PC6） were electro-acupunctured, and changes in AAI were recorded. Results： AAI significantly rose in all groups during the initial several minutes after electro-acupuncture and significantly fell in group 2 at 20 min after electro-acupuncture（P〈 0.05）.Conclusion： AAI can sensitively reflect pain response during electro-acupuncture and electro-acupuncture can strengthen propofol sedation at its medium concentration.展开更多
文摘Background： Sevoflurane and propofol are widely nsed anesthetics focused on changes in protein expression properties and membrane for surgery. Studies on the mechanisms of general anesthesia have lipid. MicroRNAs （miRNAs） regulate neural function by altering protein expression. We hypothesize that sevoflurane and propofol affect miRNA expression profiles in the brain, expect to understand the mechanism of anesthetic agents. Methods： Rats were randomly assigned to a 2% sevoflurane group, 600 μg-kg -1min propofol group, and a control group without anesthesia （n = 4, respectively）. Treatment group was under anesthesia for 6 h, and all rats breathed spontaneously with continuous monitoring of respiration and blood gases. Changes in rat cortex miRNA expression profiles were analyzed by miRNA microarrays and validated by quantitative real-time polymerase chain reaction （qRT-PCR）. Differential expression of miRNA using qRT-PCR among the control, sevoflurane, and propotbl groups were compared using one-way analysis of variance （ANOVA）. Results： Of 677 preloaded rat miRNAs, the microarray detected the expression of 277 miRNAs in rat cortex （40.9%）, of which 9 were regulated by propofol and （or） sevoflurane. Expression levels of three miRNAs （rno-miR-339-3p, rno-miR-448, rno-miR-466b- 1 ＊） were significantly increased following sevoflurane and six （rno-miR-339-3p, rno-miR-347, rno-miR-378＊, rno-miR-412＊, rno-miR-702-3p, and rno-miR-7a-2＊） following propofol. Three miRNAs （rno-miR-466b-I ＊, rno-miR-3584-Sp and rno-miR-702-3p） were differentially expressed by the two anesthetic treatment groups. Conclusions： Sevoflurane and propofol anesthesia induced distinct changes in brain miRNA expression patterns, suggesting differential regulation of protein expression. Determining the targets of these differentially expressed miRNAs may help reveal both the common and agent-specific actions of anesthetics on neurological and physiological function.
文摘Objective： To investigate the influence of electroacupuncture on auditory evoked potential index （AAI） during propofol sedation. Methods： According to propofol effect site concentration, 24 patients for operation were randomly allocated to group 1 （1.0 μg/mL）, group 2 （1. 5μg/mL） and group 3 （2.0 μg/mL）. Propofol was administered intravenously, points Hegu （LI4） and Neiguan （PC6） were electro-acupunctured, and changes in AAI were recorded. Results： AAI significantly rose in all groups during the initial several minutes after electro-acupuncture and significantly fell in group 2 at 20 min after electro-acupuncture（P〈 0.05）.Conclusion： AAI can sensitively reflect pain response during electro-acupuncture and electro-acupuncture can strengthen propofol sedation at its medium concentration.
