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Natural variations in the promoter alter SGT3 gene expression and affect steroidal glycoalkaloid content in potatoes
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作者 Nanling Zhou Xiaomei Zhang +6 位作者 Ruqian Bai Chao Wang Junmei Guan Ding Fan Yi Shang Yuxin Jia Ling Ma 《Journal of Integrative Agriculture》 2025年第9期3693-3697,共5页
Highlights●Natural variations in the SGT3 promoter TATA box repeats directly modulate gene expression and SGAs content in tubers,providing a novel molecular marker for low-steroidal glycoalkaloids(SGAs)breeding.●The... Highlights●Natural variations in the SGT3 promoter TATA box repeats directly modulate gene expression and SGAs content in tubers,providing a novel molecular marker for low-steroidal glycoalkaloids(SGAs)breeding.●The SGT3 promoter haplotype with(TA)10exhibits signifcantly higher transcriptional activity,correlating with high SGAs content,while the(TA)13haplotype is linked to low SGAs in natural germplasms. 展开更多
关键词 sgt promoter natural germplasms natural variations promoteR molecular marker sgt promoter tata box repeats gene expression steroidal glycoalkaloid content
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International Symposium Themed“Strengthen the Role of the United Nations and Promote Multilateralism”Held in Beijing
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《China International Studies》 2025年第2期F0002-F0002,共1页
On March 24,the international symposium themed“Strengthen the Role of the United Nations and Promote Multilateralism”was held in Beijing.The event was hosted by the Ministry of Foreign Affairs of China,and supported... On March 24,the international symposium themed“Strengthen the Role of the United Nations and Promote Multilateralism”was held in Beijing.The event was hosted by the Ministry of Foreign Affairs of China,and supported by China Institute of International Studies(CIIS),the United Nations Association of China. 展开更多
关键词 united nations association MULTILATERALISM promote ROLE promote multilateralism united nations STRENGTHEN role united nations
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High-yield pentanes-plus production via hydrogenation of carbon dioxide:Revealing new roles of zirconia as promoter of iron catalyst with long-term stability 被引量:1
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作者 Sheraz Ahmed Junjung Rohmat Sugiarto +6 位作者 Wonjoong Yoon Muhammad Irshad Heuntae Jo Syeda Sidra Bibi Soek Ki Kim Muhammad Kashif Khan Jaehoon Kim 《Journal of Energy Chemistry》 2025年第3期431-442,共12页
The metal oxide promoter decisively influences the overall performance of Fe catalysts in the direct hydrogenation of CO_(2)to C_(5+)hydrocarbons.However,the roles of metal oxide promoter for Fe catalysts,particularly... The metal oxide promoter decisively influences the overall performance of Fe catalysts in the direct hydrogenation of CO_(2)to C_(5+)hydrocarbons.However,the roles of metal oxide promoter for Fe catalysts,particularly ZrO_(2),have rarely been investigated.To plug this knowledge gap,a new Fe catalyst promoted with Na and partially reduced ZrO_(x)(Na-FeZrO_(x-9))was developed in this study;the catalyst helped produce C_(5+)hydrocarbons in remarkably high yield(26.3%at 360℃).In contrast to ZrO_(x)-free Fe-oxide,NaFeZrO_(x)-9 exhibited long-term stability for CO_(2)hydrogenation(750 h on-stream).The findings revealed multiple roles of ZrO_(x).Notably,ZrO_(x)decorated the Fe-oxide particles after calcination,thereby suppressing excess particle aggregation during the reaction,and acted as a"coke remover"to eliminate the carbon deposited on the catalyst surface.Additionally,oxygen vacancy(O_(v))sites in ZrO_(x)and electron transfer from ZrO_(x)to Fe sites facilitated the adsorption of CO_(2)at the Zr-Fe interface. 展开更多
关键词 CO_(2)hydrogenation C5+hydrocarbons Fe catalysts ZrO_(2)promoter Fischer Tropsch synthesis Catalyst deactivation
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Roles of Sn-promoter and carbon nanotubes treatment on supported CoB catalysts for hydrogen production
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作者 SHI Limin LI Yanbo +2 位作者 LEI Qiang REN Rongzhi WANG Yujing 《燃料化学学报(中英文)》 北大核心 2025年第5期703-712,共10页
Carbon nanotubes(CNTs)supported CoB and CoBSn catalysts were synthesized for hydrogen production via NaBH4 hydrolysis.The roles of Sn-promoter and the effect of CNTs treatment on CoB catalysts were evaluated and discu... Carbon nanotubes(CNTs)supported CoB and CoBSn catalysts were synthesized for hydrogen production via NaBH4 hydrolysis.The roles of Sn-promoter and the effect of CNTs treatment on CoB catalysts were evaluated and discussed.It is found that after the addition of Sn promoter,the specific surface area and the generation of active CoB phase are increased,while the oxidation treatment of CNTs results in more loading amounts of active components and enrichment of electron at active sites as well as large surface area.Consequently,the Sn-doped CoB catalysts supported on CNTs with oxidation treatment exhibits a significantly improved activity with a high H_(2)generation rate of 2640 mL/(min·g).Meanwhile,this catalyst shows a low activation energy of 43.7 kJ/mol and relatively high reusability. 展开更多
关键词 sodium borohydride hydrolysis CoB-based catalysts Sn promoter carbon nanotubes oxidation treatment
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Re-recognizing W/Mo promoter as a transformer changing inactive V into highly active catalytic species distribution
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作者 Qi-Ling Qian Zhen-Yun Zeng +4 位作者 Wan-Ting Chen Yu Cheng Jia Zhang Chuan-Hua Li Guang-Ren Qian 《Tungsten》 2025年第4期887-897,共11页
The promoter is very important for effective catalysts,and the relationship among active species,promoter,and support is a hot topic in the field of catalysis.This work aims to investigate the influence of the promote... The promoter is very important for effective catalysts,and the relationship among active species,promoter,and support is a hot topic in the field of catalysis.This work aims to investigate the influence of the promoter on the formation of effective active species.For this purpose,V was leached out from V-loaded TiO2(V/Ti)step by step,and the loaded V was classified into different species based on their performances in the catalytic oxidation of chlorobenzene.As a result,when V/Ti was co-doped with Mo or W,the chlorobenzene conversion rate was increased from 48.1%to 54.0%(Mo)or 61.0%(W)at210℃.The enhancement was attributed to the redistribution of V species.Specifically,7.8%of V was"Inactive"V on V/Ti.This"Inactive"V was transformed into an"Active"species.At the same time,"Highly-active V"amounts were increased from 86.0%(V_(3.3)/Ti)to 88.1%(WV_(3.3)/Ti)and 87.1%(MoV_(3.3)/Ti).According to the mechanism investigation,when W and Mo coexisted with V during the synthesis,V3+was oxidized into V^(5+),which is easily invaded into the TiO_(2),created more strong acidic sites,and activated more active lattice oxygens.These are all in favor of the effective catalytic oxidation of chlorobenzene.The new understanding of the promoter effect will help to produce a more effective catalyst using the same amount of active species. 展开更多
关键词 TUNGSTEN promoteR Vanadium species Titanium dioxide Catalytic oxidization CHLOROBENZENE
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Numerical Evaluation of the Performance Enhancement of S-Shaped Diffuser at the Intake of Gas Turbine by Energy Promoters
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作者 Hussain H.Al-Kayiem Raed A.Jessam +1 位作者 Sinan S.Hamdi Ali M.Tukkee 《Energy Engineering》 2025年第4期1311-1335,共25页
Size reduction of the gas turbines(GT)by reducing the inlet S-shaped diffuser length increases the powerto-weight ratio.It improves the techno-economic features of the GT by lesser fuel consumption.However,this Length... Size reduction of the gas turbines(GT)by reducing the inlet S-shaped diffuser length increases the powerto-weight ratio.It improves the techno-economic features of the GT by lesser fuel consumption.However,this Length reduction of a bare S-shaped diffuser to an aggressive S-shaped diffuser would risk flow separation and performance reduction of the diffuser and the air intake of the GT.The objective of this research is to propose and assess fitted energy promoters(EPs)to enhance the S-shaped diffuser performance by controlling and modifying the flow in the high bending zone of the diffuser.After experimental assessment,the work has been extended to cover more cases by numerical investigations on bare,bare aggressive,and aggressive with energy promoters S-shaped diffusers.Three types of EPs,namely co-rotating low-profile,co-rotating streamline sheet,and trapezoidal submerged EPs were tested with various combinations over a range of Reynolds numbers from 40,000 to 75,000.The respective S-shaped diffusers were simulated by computational fluid dynamics(CFD)using ANSYS software adopting a steady,3D,standard k-εturbulence model to acquire the details of the flow structure,which cannot be visualized in the experiment.The diffuser performance has been evaluated by the performance indicators of static pressure recovery coefficient,total pressure loss coefficient,and distortion coefficient(DC(45°)).The enhancements in the static pressure recovery of the S-shaped aggressive diffuser with energy promoters are 19.5%,22.2%,and 24.5%with EPs at planes 3,4 and 5,respectively,compared to the aggressive bare diffuser.In addition,the installation of the EPs resulted in a DC(45°)reduction at the outlet plane of the diffuser of about 43%at Re=40,000.The enhancements in the performance parameters confirm that aggravating the internal flow eliminates the flow separation and enhances the GT intake efficiency. 展开更多
关键词 Energy promoters distortion coefficient gas turbine S-shaped diffuser static pressure recovery total pressure loss
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Methylation status of leptin gene promoter in relatively lean Chinese adults with prediabetes and type 2 diabetes mellitus
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作者 Shi-Qi Sun Sheng-Ze Liang +1 位作者 Qi Huang Jia-Zhong Sun 《World Journal of Diabetes》 2025年第12期171-178,共8页
BACKGROUND Epigenetic regulation of leptin(LEP)plays a critical role in metabolic disorders,yet its promoter methylation patterns in lean diabetic populations remain poorly characterized.Emerging evidence suggests DNA... BACKGROUND Epigenetic regulation of leptin(LEP)plays a critical role in metabolic disorders,yet its promoter methylation patterns in lean diabetic populations remain poorly characterized.Emerging evidence suggests DNA methylation may precede clinical hyperglycemia,offering potential for early risk stratification.While obesity-associated LEP methylation is well-studied,lean Asian populations who exhibit high diabetes prevalence despite lower adiposity,represent an underexplored cohort.This study hypothesizes that LEP promoter methylation in peripheral leukocytes decreases progressively from normoglycemia to prediabetes and type 2 diabetes mellitus(T2DM),correlating inversely with serum LEP levels in lean Chinese adults[body mass index(BMI)<24 kg/m^(2)].AIM To investigate LEP promoter methylation status and its association with serum LEP levels across glycemic states in lean Chinese adults.METHODS We enrolled 392 participants including 120 normoglycemic controls,94 prediabetes[44 impaired fasting glucose(IFG)/50 impaired glucose tolerance(IGT)],178 T2DM aged 40-60 years with BMI<24 kg/m^(2).Genomic DNA from peripheral leukocytes underwent bisulfite conversion followed by methylation-specific PCR to assess CpG methylation in the LEP promoter.Serum LEP was quantified via enzyme-linked immunosorbent assay,with other parameters measured through standard assays.Statistical analyses included analysis of variance,χ²tests,and Pearson correlation(Bonferroni-corrected P value).RESULTS Methylation frequencies declined progressively:59.2%(controls)reduced to 43.6%(prediabetes;IFG:38.6%,IGT:48%)reduced to 31.5%(T2DM)(all P<0.05 vs controls;T2DM vs IGT:P=0.030).Serum LEP levels increased significantly in T2DM(16.94±4.19μg/L)vs controls(11.33±3.10μg/L;P=0.002),with intermediate values in prediabetes(IFG:13.79±3.32μg/L;IGT:12.62±4.81μg/L).A near-perfect inverse correlation between methylation and LEP levels was observed(r=-0.95,95%CI:-0.97 to-0.92,P<0.001),persisting after adjusting for age and BMI(β=-0.91,P<0.001).CONCLUSION LEP promoter hypomethylation parallels worsening glycemic status in lean Chinese adults,suggesting its potential as a blood-based epigenetic biomarker for diabetes progression,pending validation in longitudinal cohorts. 展开更多
关键词 Leptin gene promoter DNA methylation Type 2 diabetes mellitus PREDIABETES Lean population Epigenetic biomarker Methylation-specific PCR
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Variation in the promoter of OsNPF7.1 contributes to nitrate uptake,remobilization,and grain yield in upland rice
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作者 Ming Yan Huimin Feng +13 位作者 Mian Gu Hanwei Mei Lei Wang Kai Xu Shoujun Chen Anning Zhang Liguo Zhou Xiaoyan Xu Peiqing Fan Liang Chen Fangjun Feng Guohua Xu Lijun Luo Hui Xia 《Journal of Genetics and Genomics》 2025年第7期954-957,共4页
Asian cultivated rice is one of the most important cereal crops globally,feeding approximately 50%of the world's population.Increasing rice nitrogen use efficiency(NUE)is crucial for achieving high yields with low... Asian cultivated rice is one of the most important cereal crops globally,feeding approximately 50%of the world's population.Increasing rice nitrogen use efficiency(NUE)is crucial for achieving high yields with low nitrogen inputs(Xu et al.,2012;Hu et al.,2023).However,modern cultivars are typically bred for high yields through excessive nitrogen fertilizer use,leading to the loss of beneficial alleles associated with high NUE during the breeding process(Wang and Peng,2017;Hu et al.,2023).Genetic improvement for high NUE should be a key strategy in breeding“Green Super Rice”(GSR)(Yu et al.,2021)and water-saving and drought-resistance rice(WDR)(Luo,2010;Xia et al.,2022)for sustainable agriculture.Asian cultivated rice is highly diverse and harbors vital genetic variants essential for adaptation to different environments(Wing et al.,2018). 展开更多
关键词 nitrate uptake osnpf grain yield nitrogen use efficiency upland rice promoter variation breeding process wang Asian cultivated rice
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Correction:Cholesterol promotes autoimmune pathology through T follicular helper cells
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作者 Wei Li George C.Tsokos 《Cellular & Molecular Immunology》 2025年第11期1519-1519,共1页
Correction to:Cellular&Molecular Immunology https://doi.org/10.1038/s41423-025-01319-1,published online 10 July 2025 The article“Cholesterol promotes autoimmune pathology through T follicular helper cells”,writt... Correction to:Cellular&Molecular Immunology https://doi.org/10.1038/s41423-025-01319-1,published online 10 July 2025 The article“Cholesterol promotes autoimmune pathology through T follicular helper cells”,written by Wei Li&George C.Tsokos,was originally published open access under a Creative Commons Attribution 4.0 International License. 展开更多
关键词 promotes autoimmune pathology molecular immunology T follicular helper cells cellular immunology CHOLESTEROL t follicular helper cells written autoimmune pathology
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Clean and tunable fabrication of ternary graphene-cobalt based promoter by chemical vapor deposition for lithium-sulfur batteries
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作者 Hua Gao Yunfeng Zhang +6 位作者 Chaoxu Hao Bo Zhao Menglei Wang Haina Ci Lixian Song Zhongyuan Lu Yingze Song 《Journal of Energy Chemistry》 2025年第8期194-202,共9页
Graphene-metal based materials have been utilized in lithium-sulfur(Li–S)batteries owing to their integrated functionalities thus far.However,their synthesis has predominantly relied on wet-chemistry routes,which lim... Graphene-metal based materials have been utilized in lithium-sulfur(Li–S)batteries owing to their integrated functionalities thus far.However,their synthesis has predominantly relied on wet-chemistry routes,which limited their practical activity in Li–S reaction systems.In this study,we introduce a chemical vapor deposition(CVD)-triggered dry-chemistry approach for the preparation of graphene-cobalt(Co)based catalysts.The versatile CVD technique provides a dry and controllable reaction environment,effectively pledging the compact and clean catalytic interfaces between graphene and Co-based components.Additionally,programmed reactions introduce defects such as vacancies and nitrogen heteroatoms into the catalysts.Notably,the graphene layer number and Co valence state can be delicately manipulated by altering the CVD reaction temperature.Specifically,few-layer graphene wrapped Co/Co_(3)O_(4)(FGr-Co/Co_(3)O_(4))prepared at 450 ℃ shows higher catalytic activity than the multi-layer graphene wrapped Co/CoO(MGr-Co/CoO)synthesized at 550 ℃,attributed to its comprehensive control of clean interface,valence distribution range and defects.Leveraging these advantages,the battery with FGr-Co/Co_(3)O_(4)shows favorable working stability with a degradation rate of only 0.08%over 500 cycles at 1.0 C.Furthermore,under an elevated sulfur loading of 6.1 mg cm^(–2),the battery harvests a remarkable areal capacity of 5.9 mA h cm^(–2)along with stable cyclic operation. 展开更多
关键词 Lithium-sulfur battery Chemical vapor deposition Graphene wrapped Co-based promoter Graphene layer control Co valence state altering
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Local abaloparatide administration promotes in situ alveolar bone augmentation via FAK-mediated periosteal osteogenesis
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作者 Ruyi Wang Yuan Li +9 位作者 Bowen Tan Shijia Li Yanting Wu Yao Chen Yuran Qian Haochen Wang Bo Li Zhihe Zhao Quan Yuan Yu Li 《International Journal of Oral Science》 2025年第6期850-863,共14页
Insufficient alveolar bone thickness increases the risk of periodontal dehiscence and fenestration,especially in orthodontic tooth movement.Abaloparatide(ABL),a synthetic analog of human PTHr P(1–34)and a clinical me... Insufficient alveolar bone thickness increases the risk of periodontal dehiscence and fenestration,especially in orthodontic tooth movement.Abaloparatide(ABL),a synthetic analog of human PTHr P(1–34)and a clinical medication for treating osteoporosis,has recently demonstrated its potential in enhancing craniofacial bone formation.Herein,we show that intraoral submucosal injection of ABL,when combined with mechanical force,promotes in situ alveolar bone thickening.The newly formed bone is primarily located outside the original compact bone,implying its origin from the periosteum.RNA sequencing of the alveolar bone tissue revealed that the focal adhesion(FA)pathway potentially mediates this bioprocess.Local injection of ABL alone enhances cell proliferation,collagen synthesis,and phosphorylation of focal adhesion kinase(FAK)in the alveolar periosteum;when ABL is combined with mechanical force,the FAK expression is upregulated,in line with the accomplishment of the ossification.In vitro,ABL enhances proliferation,migration,and FAK phosphorylation in periosteal stem cells.Furthermore,the pro-osteogenic effects of ABL on alveolar bone are entirely blocked when FAK activity is inhibited by a specific inhibitor.In summary,abaloparatide combined with mechanical force promotes alveolar bone formation via FAK-mediated periosteal osteogenesis.Thus,we have introduced a promising therapeutic approach for drug-induced in situ alveolar bone augmentation,which may prevent or repair the detrimental periodontal dehiscence,holding significant potential in dentistry. 展开更多
关键词 FAK Local abaloparatide administration promotes in situ alveolar bone augmentation via FAK-mediated periosteal osteogenesis
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LncRNA EUDAL shapes tumor cell response to hypoxia-induced constitutive EGFR activation and promotes chemoresistance in oral cancer
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作者 Shengkai Chen Zhenlin Dai +3 位作者 Jianbo Shi Mengyu Rui Zhiyuan Zhang Qin Xu 《International Journal of Oral Science》 2025年第6期864-879,共16页
Hypoxia and aberrant activation of epidermal growth factor receptor(EGFR)are considered important features of various malignancies.However,whether hypoxia can directly trigger EGFR activation and its clinical implicat... Hypoxia and aberrant activation of epidermal growth factor receptor(EGFR)are considered important features of various malignancies.However,whether hypoxia can directly trigger EGFR activation and its clinical implications remain unclear.In this study,we demonstrated that in oral cancer,a typical hypoxic tumor,hypoxia can induce chronic but constitutive phosphorylation of wild-type EGFR in the absence of ligands.Oral cancer cell lines exhibit different EGFR phosphorylation responses to hypoxia.In hypoxic HN4 and HN6 cells,ubiquitination-mediated endocytosis,lysosomal sorting,and degradation lead to low levels of EGFR phosphorylation.However,in CAL-27 and HN30 cells,a novel HIF-1α-induced long noncoding RNA(lnc RNA),EUDAL,can compete with the E3 ligase/adaptor complex c-Cbl/Grb2 for binding to EGFR,stabilizing phosphorylated EGFR(p EGFR)and resulting in sustained activation of EGFR and its downstream STAT3/BNIP3 signaling.STAT3/BNIP3-mediated autophagy leads to antitumor drug resistance.A high EUDAL/EGFR/STAT3/autophagy pathway activation predicts poor response to chemotherapy in oral cancer patients.Collectively,hypoxia can induce noncanonical ligand-independent EGFR phosphorylation.High EUDAL expression facilitates sustained EGFR phosphorylation in hypoxic tumor cells and leads to autophagy-related drug resistance. 展开更多
关键词 LncRNA EUDAL shapes tumor cell response to hypoxia-induced constitutive EGFR activation and promotes chemoresistance in oral can
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Promoter effect on the CO_2-H_2O formation during Fischer-Tropsch synthesis on iron-based catalysts 被引量:1
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作者 Ali Nakhaei Pour Seyed Mehdi Kamali Shahri +1 位作者 Yahya Zamani Akbar Zamanian 《Journal of Natural Gas Chemistry》 EI CAS CSCD 2010年第2期193-197,共5页
The effects of Mg,La and Ca promoters on primary and secondary CO2 and H2O formation pathways during Fischer-Tropsch synthesis on precipitated Fe/Cu/SiO2 catalysts are investigated.The chemisorbed oxygen atoms in the ... The effects of Mg,La and Ca promoters on primary and secondary CO2 and H2O formation pathways during Fischer-Tropsch synthesis on precipitated Fe/Cu/SiO2 catalysts are investigated.The chemisorbed oxygen atoms in the primary pathway formed in the CO dissociation steps reacted with co-adsorbed hydrogen or carbon monoxide to produce H2O and CO2,respectively.The secondary pathway was the water-gas shift reaction.The results indicated that the CO2 production led to an increase in both primary and secondary pathways,and H2O production decreased when surface basicity of the catalyst increased in the order Ca 〉 Mg 〉 La. 展开更多
关键词 Ca promoter Mg promoter La promoter Fischer-Tropsch synthesis iron-based catalyst water-gas shift reaction
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牙鲆GHR基因Promoter区微卫星序列多态性与生长性状关系的初步研究 被引量:10
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作者 倪静 尤锋 +5 位作者 于深辉 张培军 徐冬冬 吴志昊 文爱韵 徐永立 《中国海洋大学学报(自然科学版)》 CAS CSCD 北大核心 2008年第5期719-725,共7页
采用牙鲆GHR基因5’端Promoter区的1个微卫星标记,对胶南和日照2个牙鲆养殖群体进行了群体遗传多样性的研究,并探索该基因多态性位点与牙鲆生长性状之间的相关性。结果表明,2个群体在该座位的等位基因数为12和9个,有效等位基因数为6.26... 采用牙鲆GHR基因5’端Promoter区的1个微卫星标记,对胶南和日照2个牙鲆养殖群体进行了群体遗传多样性的研究,并探索该基因多态性位点与牙鲆生长性状之间的相关性。结果表明,2个群体在该座位的等位基因数为12和9个,有效等位基因数为6.26和5.04个,多态信息含量为0.84和0.80。2个群体该座位的Hardy-Weinberg遗传偏离指数均为正值,并没有显示出杂合子缺失,但各基因型分布频率都在一定程度上偏离Hardy-Weinberg平衡(P<0.01)。连锁分析中发现,在胶南群体中,IM基因型对应的个体在全重、全长、体长、头长、体高和眼径形态学数据中均是最大的;在日照群体中,BC基因型对应的个体在全重、全长、体高、尾柄高、尾柄长和眼径数据中均是最大的;而CJ基因型对应的个体在体长和头长这两组数据中是最大的。该结果为研究GHR基因的变异对鱼类生长发育的影响及探索将该基因作为生长遗传标记的可行性奠定了实验基础。 展开更多
关键词 牙鲆 GHR基因5’端promoter区 微卫星 遗传多样性 性状连锁分析
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Isolation and Analysis of Rubber Hevein Gene and Its Promoter Sequence 被引量:4
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作者 邓晓东 费小雯 +1 位作者 黄俊生 郑学勤 《Acta Botanica Sinica》 CSCD 2002年第8期936-940,共5页
Hevein, a lectin_like protein, is a major factor of lutoids in the latex of rubber trees ( Hevea brasiliensis Muell._Arg.). This factor is involved in coagulation of the latex and has the ability to bind chitin. Th... Hevein, a lectin_like protein, is a major factor of lutoids in the latex of rubber trees ( Hevea brasiliensis Muell._Arg.). This factor is involved in coagulation of the latex and has the ability to bind chitin. The hevein gene with a length of 680 bp was cloned by the method of RT_PCR. Its promoter region with 1 306 bp of this gene was also isolated by genome walking, and its sequence included the typical TATA and CAAT boxes as well as the homologous sequence of abscisic acid (ABA) response elements. Expression of the hevein gene in the latex and leaves was detected by Northern blot. After treatment of the trees with ethylene and ABA, the results showed that the hevein gene was expressed principally in latex, and the expression could be induced by ethylene and ABA. 展开更多
关键词 rubber tree HEVEIN promoteR Northern blot
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Isolation of a Genomic DNA for Gastrodia Antifungal Protein and Analyses of Its Promoter in Transgenic Tobacco 被引量:3
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作者 萨其拉 王义琴 +2 位作者 李文彬 张利明 孙勇如 《Acta Botanica Sinica》 CSCD 2003年第2期229-233,共5页
A genomic DNA containing 5'-upstream region and complete open reading frame of a Gastrodia antifungal protein was isolated by screening of a genomic library from Gastrodia elata B1. To investigate the promoter act... A genomic DNA containing 5'-upstream region and complete open reading frame of a Gastrodia antifungal protein was isolated by screening of a genomic library from Gastrodia elata B1. To investigate the promoter activity, the 5'-flanking region - 1 157 lip upstream from the putative transcription start site was fused to the coding sequence of beta-glucuronidase (GUS) gene and transformed into Nicotiana tabacum. The strongest GUS activity was detected in the roots of transgenic tobacco, followed by stems. The leaves only showed a low GUS activity. Furthermore, the promoter established inducible expression pattern in transgenic tobacco upon fungus Trichoderma viride inoculation and jasmonic acid and salicylic acid treatments. 展开更多
关键词 Gastrodia elata Bl. promoteR transgenic tobacco organ-specific induced expression
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Study on Cloning and Polymorphism of Porcine Adiponectin Promoter 被引量:2
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作者 凌飞 汪亮亮 +2 位作者 杜红丽 李仕新 陈瑶生 《Agricultural Science & Technology》 CAS 2009年第6期53-56,共4页
[ Objective] The aim of this study was to provide a basis for study on adiponectin as a candidate gene for fat deposition. [ Method] The promoter sequence of adiponectin was obtained by porcine BAC library screening a... [ Objective] The aim of this study was to provide a basis for study on adiponectin as a candidate gene for fat deposition. [ Method] The promoter sequence of adiponectin was obtained by porcine BAC library screening and primer-walking method. The polymorphisms of adiponectin promoter from 290 pigs, including 5 breeds of Lantang pig, Large spotted pig, Large white pig, Landrace and Duroc, were analyzed with PCR-RFLP. [ Result] At SNP site of adiponectin 5'-flanking region -1 010 bp (G/A), GG genotype frequency in Chinese indigenous pigs was significantly higher than that in exotic pigs. At SNP site of adiponectin 5'-flanking region -394 bp (T/C), the genotype distribution of Chi- nese indigenous pigs was abundant, while no CC genotype was detected in exotic pigs, and T allele frequency was higher in exotic pigs. [ Conclusion] SNP site mutation of - 1 010 bp (G/A) may lead to changes of the gene transcription level, while SNP site of -394 bp (T/C) properly has no relationship with gene transcription level and fat deposition. 展开更多
关键词 PORCINE BAC library ADIPONECTIN promoteR SNP
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Obtaining High Pest_resistant Transgenic Upland Cotton Cultivars Carrying cry1Ac3 Gene Driven by Chimeric OM Promoter 被引量:5
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作者 陈宛新 肖桂芳 朱祯 《Acta Botanica Sinica》 CSCD 2002年第8期963-970,共8页
Hypocotyl segments from aseptic seedlings of two important cultivars of upland cotton ( Gossypium hirsutum L.) in Northwest China, 'Xinluzao_1', 'Jinmian_7', 'Jinmian_12' and 'Jihe_321&#... Hypocotyl segments from aseptic seedlings of two important cultivars of upland cotton ( Gossypium hirsutum L.) in Northwest China, 'Xinluzao_1', 'Jinmian_7', 'Jinmian_12' and 'Jihe_321' were transformed respectively by two efficient plant expression plasmids pBinMoBc and pBinoBc via Agrobacterium tumefaciens . In pBinMoBc, cry 1Ac3 gene, which encodes the Bt toxin, is under the control of chimeric OM promoter. In pBinoBc, it is under control of CaMV 35S promoter. After co_cultivation with Agrobacterium tumefimpfaciens LBA4404 (containing pBinMoBc or pBinoBc), kanamycin_resistant selection, somatic embryos were induced and regenerated plants were obtained. Then the regenerated plantlets were grafted to untransformed stocks in greenhouse to produce descendants. The integration of cry 1Ac3 gene and its expression in T 2 generation of transgenic cotton plants were confirmed by Southern hybridization and Western blotting. The analyses of insect bioassay indicated that the transgenic plants of both constructions have significant resistance to the larvae of cotton bollworm ( Heliothis armigera ) and that cry 1Ac3 gene driven by chimeric OM promoter could endue T 2 generation cotton with high pest_resistant ability, implicating that it has a profound application in genetic engineering to breed new pest_resistant cotton varieties. 展开更多
关键词 upland cotton insect_resistant cotton transgenic plant Agrobacterium tumefaciens cry 1Ac3 gene chimeric promoter
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Effects of Supports and Promoters on in situ Hydrogenation of o-Cresol over Ni-based Catalysts 被引量:1
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作者 李雁斌 徐莹 +3 位作者 马隆龙 王铁军 张琦 陈冠益 《Chinese Journal of Chemical Physics》 SCIE CAS CSCD 2014年第6期697-703,I0004,共8页
We investigated the effects of supports (CMK-3, SiO2ZrO2, MgO, Al2O3) and promoters (Cu, Ce, Fe) on textual properties of Ni based catalysts. o-Cresol was used as a probe to test the activity of these catalysts un... We investigated the effects of supports (CMK-3, SiO2ZrO2, MgO, Al2O3) and promoters (Cu, Ce, Fe) on textual properties of Ni based catalysts. o-Cresol was used as a probe to test the activity of these catalysts under the condition of 230 ℃ and nitrogen pres-sure of 0.1 MPa. The catalysts were characterized by X-ray diffraction, H2 temperature programmed reduction ammonium programmed desorption, and N2 adsorption-desorption isotherms. The results showed that the catalytic performance of Ni/CMK-3 (the conversion of o-cresol reached 45.4%) was significantly better than the other three kinds of supports. The modification of Ni/CMK-3 was also investigated and over 60% conversion of o-cresol was obtained after the addition of Ce (64.6%)and Cu (66.8%) in Ni/CMK-3, whereas the addition of Fe led to a decrease of conversion. In the meantime, Cu changed the products dis-tribution. The appearance of toluene indicated that another pathway existed in the reaction.Accompanied by the ascension of conversion in both sides, side effects also occurred and got more serious. The apparent order of activity for all the tested catalysts was NiCe/CMK-3〉NiCu/CMK-3〉Ni/CMK-3〉NiFe/CMK-3〉Ni/Al2O3Ni/SiO2ZrO2〉Ni/MgO. The reac-tion pathway, involving three routes, was also mentioned in this study. 展开更多
关键词 in-situ hydrogenation SUPPORT promoteR O-CRESOL
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Isolation and Characterization of CMO Gene Promoter from Halophyte Suaeda liaotungensis K. 被引量:1
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作者 李秋莉 尹辉 +3 位作者 李丹 朱宏飞 张毅 朱巍巍 《Journal of Genetics and Genomics》 SCIE CAS CSCD 北大核心 2007年第4期355-361,共7页
The 5'-flanking proximal region of stress-induced gene encoding choline monooxygenase (CMO) was isolated by Adaptor-PCR and TAIL-PCR from halophyte Suaeda liaotungensis K. A total of 2,204 bp DNA sequence was obtai... The 5'-flanking proximal region of stress-induced gene encoding choline monooxygenase (CMO) was isolated by Adaptor-PCR and TAIL-PCR from halophyte Suaeda liaotungensis K. A total of 2,204 bp DNA sequence was obtained. The transcription start site, which is located at 128 bp upstream to the start ATG, was predicted by the TSSP-TCM program. The functional elements were analysed by PLACE program. The obtained SICMO gene promoter contains the basic elements: TATA-box, CAAT-box, and stress-induced elements, for example, salt responsive element (GAAAAA), cold responsive elements (CANNTG), ABA (Abscisic Acid) responsive elements (NAACAA), water stress element (CGGTTG), and WUN responsive elements (GTTAGGTTC). Isolation and analysis of the promoter of the CMO gene from S. liaotungensis lays a foundation for characterising the stress-induced promoter elements, studying the relationship between the structure and function of the promoter, and investigating the molecular mechanism of CMO gene regulation. 展开更多
关键词 Suaeda liaotungensis K. choline monooxygenase (CMO) promoteR
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