期刊文献+
共找到2,370篇文章
< 1 2 119 >
每页显示 20 50 100
Natural variations in the promoter alter SGT3 gene expression and affect steroidal glycoalkaloid content in potatoes
1
作者 Nanling Zhou Xiaomei Zhang +6 位作者 Ruqian Bai Chao Wang Junmei Guan Ding Fan Yi Shang Yuxin Jia Ling Ma 《Journal of Integrative Agriculture》 2025年第9期3693-3697,共5页
Highlights●Natural variations in the SGT3 promoter TATA box repeats directly modulate gene expression and SGAs content in tubers,providing a novel molecular marker for low-steroidal glycoalkaloids(SGAs)breeding.●The... Highlights●Natural variations in the SGT3 promoter TATA box repeats directly modulate gene expression and SGAs content in tubers,providing a novel molecular marker for low-steroidal glycoalkaloids(SGAs)breeding.●The SGT3 promoter haplotype with(TA)10exhibits signifcantly higher transcriptional activity,correlating with high SGAs content,while the(TA)13haplotype is linked to low SGAs in natural germplasms. 展开更多
关键词 sgt promoter natural germplasms natural variations promoteR molecular marker sgt promoter tata box repeats gene expression steroidal glycoalkaloid content
在线阅读 下载PDF
International Symposium Themed“Strengthen the Role of the United Nations and Promote Multilateralism”Held in Beijing
2
《China International Studies》 2025年第2期F0002-F0002,共1页
On March 24,the international symposium themed“Strengthen the Role of the United Nations and Promote Multilateralism”was held in Beijing.The event was hosted by the Ministry of Foreign Affairs of China,and supported... On March 24,the international symposium themed“Strengthen the Role of the United Nations and Promote Multilateralism”was held in Beijing.The event was hosted by the Ministry of Foreign Affairs of China,and supported by China Institute of International Studies(CIIS),the United Nations Association of China. 展开更多
关键词 united nations association MULTILATERALISM promote ROLE promote multilateralism united nations STRENGTHEN role united nations
在线阅读 下载PDF
High-yield pentanes-plus production via hydrogenation of carbon dioxide:Revealing new roles of zirconia as promoter of iron catalyst with long-term stability 被引量:1
3
作者 Sheraz Ahmed Junjung Rohmat Sugiarto +6 位作者 Wonjoong Yoon Muhammad Irshad Heuntae Jo Syeda Sidra Bibi Soek Ki Kim Muhammad Kashif Khan Jaehoon Kim 《Journal of Energy Chemistry》 2025年第3期431-442,共12页
The metal oxide promoter decisively influences the overall performance of Fe catalysts in the direct hydrogenation of CO_(2)to C_(5+)hydrocarbons.However,the roles of metal oxide promoter for Fe catalysts,particularly... The metal oxide promoter decisively influences the overall performance of Fe catalysts in the direct hydrogenation of CO_(2)to C_(5+)hydrocarbons.However,the roles of metal oxide promoter for Fe catalysts,particularly ZrO_(2),have rarely been investigated.To plug this knowledge gap,a new Fe catalyst promoted with Na and partially reduced ZrO_(x)(Na-FeZrO_(x-9))was developed in this study;the catalyst helped produce C_(5+)hydrocarbons in remarkably high yield(26.3%at 360℃).In contrast to ZrO_(x)-free Fe-oxide,NaFeZrO_(x)-9 exhibited long-term stability for CO_(2)hydrogenation(750 h on-stream).The findings revealed multiple roles of ZrO_(x).Notably,ZrO_(x)decorated the Fe-oxide particles after calcination,thereby suppressing excess particle aggregation during the reaction,and acted as a"coke remover"to eliminate the carbon deposited on the catalyst surface.Additionally,oxygen vacancy(O_(v))sites in ZrO_(x)and electron transfer from ZrO_(x)to Fe sites facilitated the adsorption of CO_(2)at the Zr-Fe interface. 展开更多
关键词 CO_(2)hydrogenation C5+hydrocarbons Fe catalysts ZrO_(2)promoter Fischer Tropsch synthesis Catalyst deactivation
在线阅读 下载PDF
Roles of Sn-promoter and carbon nanotubes treatment on supported CoB catalysts for hydrogen production
4
作者 SHI Limin LI Yanbo +2 位作者 LEI Qiang REN Rongzhi WANG Yujing 《燃料化学学报(中英文)》 北大核心 2025年第5期703-712,共10页
Carbon nanotubes(CNTs)supported CoB and CoBSn catalysts were synthesized for hydrogen production via NaBH4 hydrolysis.The roles of Sn-promoter and the effect of CNTs treatment on CoB catalysts were evaluated and discu... Carbon nanotubes(CNTs)supported CoB and CoBSn catalysts were synthesized for hydrogen production via NaBH4 hydrolysis.The roles of Sn-promoter and the effect of CNTs treatment on CoB catalysts were evaluated and discussed.It is found that after the addition of Sn promoter,the specific surface area and the generation of active CoB phase are increased,while the oxidation treatment of CNTs results in more loading amounts of active components and enrichment of electron at active sites as well as large surface area.Consequently,the Sn-doped CoB catalysts supported on CNTs with oxidation treatment exhibits a significantly improved activity with a high H_(2)generation rate of 2640 mL/(min·g).Meanwhile,this catalyst shows a low activation energy of 43.7 kJ/mol and relatively high reusability. 展开更多
关键词 sodium borohydride hydrolysis CoB-based catalysts Sn promoter carbon nanotubes oxidation treatment
在线阅读 下载PDF
Numerical Evaluation of the Performance Enhancement of S-Shaped Diffuser at the Intake of Gas Turbine by Energy Promoters
5
作者 Hussain H.Al-Kayiem Raed A.Jessam +1 位作者 Sinan S.Hamdi Ali M.Tukkee 《Energy Engineering》 2025年第4期1311-1335,共25页
Size reduction of the gas turbines(GT)by reducing the inlet S-shaped diffuser length increases the powerto-weight ratio.It improves the techno-economic features of the GT by lesser fuel consumption.However,this Length... Size reduction of the gas turbines(GT)by reducing the inlet S-shaped diffuser length increases the powerto-weight ratio.It improves the techno-economic features of the GT by lesser fuel consumption.However,this Length reduction of a bare S-shaped diffuser to an aggressive S-shaped diffuser would risk flow separation and performance reduction of the diffuser and the air intake of the GT.The objective of this research is to propose and assess fitted energy promoters(EPs)to enhance the S-shaped diffuser performance by controlling and modifying the flow in the high bending zone of the diffuser.After experimental assessment,the work has been extended to cover more cases by numerical investigations on bare,bare aggressive,and aggressive with energy promoters S-shaped diffusers.Three types of EPs,namely co-rotating low-profile,co-rotating streamline sheet,and trapezoidal submerged EPs were tested with various combinations over a range of Reynolds numbers from 40,000 to 75,000.The respective S-shaped diffusers were simulated by computational fluid dynamics(CFD)using ANSYS software adopting a steady,3D,standard k-εturbulence model to acquire the details of the flow structure,which cannot be visualized in the experiment.The diffuser performance has been evaluated by the performance indicators of static pressure recovery coefficient,total pressure loss coefficient,and distortion coefficient(DC(45°)).The enhancements in the static pressure recovery of the S-shaped aggressive diffuser with energy promoters are 19.5%,22.2%,and 24.5%with EPs at planes 3,4 and 5,respectively,compared to the aggressive bare diffuser.In addition,the installation of the EPs resulted in a DC(45°)reduction at the outlet plane of the diffuser of about 43%at Re=40,000.The enhancements in the performance parameters confirm that aggravating the internal flow eliminates the flow separation and enhances the GT intake efficiency. 展开更多
关键词 Energy promoters distortion coefficient gas turbine S-shaped diffuser static pressure recovery total pressure loss
在线阅读 下载PDF
Methylation status of leptin gene promoter in relatively lean Chinese adults with prediabetes and type 2 diabetes mellitus
6
作者 Shi-Qi Sun Sheng-Ze Liang +1 位作者 Qi Huang Jia-Zhong Sun 《World Journal of Diabetes》 2025年第12期171-178,共8页
BACKGROUND Epigenetic regulation of leptin(LEP)plays a critical role in metabolic disorders,yet its promoter methylation patterns in lean diabetic populations remain poorly characterized.Emerging evidence suggests DNA... BACKGROUND Epigenetic regulation of leptin(LEP)plays a critical role in metabolic disorders,yet its promoter methylation patterns in lean diabetic populations remain poorly characterized.Emerging evidence suggests DNA methylation may precede clinical hyperglycemia,offering potential for early risk stratification.While obesity-associated LEP methylation is well-studied,lean Asian populations who exhibit high diabetes prevalence despite lower adiposity,represent an underexplored cohort.This study hypothesizes that LEP promoter methylation in peripheral leukocytes decreases progressively from normoglycemia to prediabetes and type 2 diabetes mellitus(T2DM),correlating inversely with serum LEP levels in lean Chinese adults[body mass index(BMI)<24 kg/m^(2)].AIM To investigate LEP promoter methylation status and its association with serum LEP levels across glycemic states in lean Chinese adults.METHODS We enrolled 392 participants including 120 normoglycemic controls,94 prediabetes[44 impaired fasting glucose(IFG)/50 impaired glucose tolerance(IGT)],178 T2DM aged 40-60 years with BMI<24 kg/m^(2).Genomic DNA from peripheral leukocytes underwent bisulfite conversion followed by methylation-specific PCR to assess CpG methylation in the LEP promoter.Serum LEP was quantified via enzyme-linked immunosorbent assay,with other parameters measured through standard assays.Statistical analyses included analysis of variance,χ²tests,and Pearson correlation(Bonferroni-corrected P value).RESULTS Methylation frequencies declined progressively:59.2%(controls)reduced to 43.6%(prediabetes;IFG:38.6%,IGT:48%)reduced to 31.5%(T2DM)(all P<0.05 vs controls;T2DM vs IGT:P=0.030).Serum LEP levels increased significantly in T2DM(16.94±4.19μg/L)vs controls(11.33±3.10μg/L;P=0.002),with intermediate values in prediabetes(IFG:13.79±3.32μg/L;IGT:12.62±4.81μg/L).A near-perfect inverse correlation between methylation and LEP levels was observed(r=-0.95,95%CI:-0.97 to-0.92,P<0.001),persisting after adjusting for age and BMI(β=-0.91,P<0.001).CONCLUSION LEP promoter hypomethylation parallels worsening glycemic status in lean Chinese adults,suggesting its potential as a blood-based epigenetic biomarker for diabetes progression,pending validation in longitudinal cohorts. 展开更多
关键词 Leptin gene promoter DNA methylation Type 2 diabetes mellitus PREDIABETES Lean population Epigenetic biomarker Methylation-specific PCR
暂未订购
Variation in the promoter of OsNPF7.1 contributes to nitrate uptake,remobilization,and grain yield in upland rice
7
作者 Ming Yan Huimin Feng +13 位作者 Mian Gu Hanwei Mei Lei Wang Kai Xu Shoujun Chen Anning Zhang Liguo Zhou Xiaoyan Xu Peiqing Fan Liang Chen Fangjun Feng Guohua Xu Lijun Luo Hui Xia 《Journal of Genetics and Genomics》 2025年第7期954-957,共4页
Asian cultivated rice is one of the most important cereal crops globally,feeding approximately 50%of the world's population.Increasing rice nitrogen use efficiency(NUE)is crucial for achieving high yields with low... Asian cultivated rice is one of the most important cereal crops globally,feeding approximately 50%of the world's population.Increasing rice nitrogen use efficiency(NUE)is crucial for achieving high yields with low nitrogen inputs(Xu et al.,2012;Hu et al.,2023).However,modern cultivars are typically bred for high yields through excessive nitrogen fertilizer use,leading to the loss of beneficial alleles associated with high NUE during the breeding process(Wang and Peng,2017;Hu et al.,2023).Genetic improvement for high NUE should be a key strategy in breeding“Green Super Rice”(GSR)(Yu et al.,2021)and water-saving and drought-resistance rice(WDR)(Luo,2010;Xia et al.,2022)for sustainable agriculture.Asian cultivated rice is highly diverse and harbors vital genetic variants essential for adaptation to different environments(Wing et al.,2018). 展开更多
关键词 nitrate uptake osnpf grain yield nitrogen use efficiency upland rice promoter variation breeding process wang Asian cultivated rice
原文传递
Clean and tunable fabrication of ternary graphene-cobalt based promoter by chemical vapor deposition for lithium-sulfur batteries
8
作者 Hua Gao Yunfeng Zhang +6 位作者 Chaoxu Hao Bo Zhao Menglei Wang Haina Ci Lixian Song Zhongyuan Lu Yingze Song 《Journal of Energy Chemistry》 2025年第8期194-202,共9页
Graphene-metal based materials have been utilized in lithium-sulfur(Li–S)batteries owing to their integrated functionalities thus far.However,their synthesis has predominantly relied on wet-chemistry routes,which lim... Graphene-metal based materials have been utilized in lithium-sulfur(Li–S)batteries owing to their integrated functionalities thus far.However,their synthesis has predominantly relied on wet-chemistry routes,which limited their practical activity in Li–S reaction systems.In this study,we introduce a chemical vapor deposition(CVD)-triggered dry-chemistry approach for the preparation of graphene-cobalt(Co)based catalysts.The versatile CVD technique provides a dry and controllable reaction environment,effectively pledging the compact and clean catalytic interfaces between graphene and Co-based components.Additionally,programmed reactions introduce defects such as vacancies and nitrogen heteroatoms into the catalysts.Notably,the graphene layer number and Co valence state can be delicately manipulated by altering the CVD reaction temperature.Specifically,few-layer graphene wrapped Co/Co_(3)O_(4)(FGr-Co/Co_(3)O_(4))prepared at 450 ℃ shows higher catalytic activity than the multi-layer graphene wrapped Co/CoO(MGr-Co/CoO)synthesized at 550 ℃,attributed to its comprehensive control of clean interface,valence distribution range and defects.Leveraging these advantages,the battery with FGr-Co/Co_(3)O_(4)shows favorable working stability with a degradation rate of only 0.08%over 500 cycles at 1.0 C.Furthermore,under an elevated sulfur loading of 6.1 mg cm^(–2),the battery harvests a remarkable areal capacity of 5.9 mA h cm^(–2)along with stable cyclic operation. 展开更多
关键词 Lithium-sulfur battery Chemical vapor deposition Graphene wrapped Co-based promoter Graphene layer control Co valence state altering
在线阅读 下载PDF
Promoter effect on the CO_2-H_2O formation during Fischer-Tropsch synthesis on iron-based catalysts 被引量:1
9
作者 Ali Nakhaei Pour Seyed Mehdi Kamali Shahri +1 位作者 Yahya Zamani Akbar Zamanian 《Journal of Natural Gas Chemistry》 EI CAS CSCD 2010年第2期193-197,共5页
The effects of Mg,La and Ca promoters on primary and secondary CO2 and H2O formation pathways during Fischer-Tropsch synthesis on precipitated Fe/Cu/SiO2 catalysts are investigated.The chemisorbed oxygen atoms in the ... The effects of Mg,La and Ca promoters on primary and secondary CO2 and H2O formation pathways during Fischer-Tropsch synthesis on precipitated Fe/Cu/SiO2 catalysts are investigated.The chemisorbed oxygen atoms in the primary pathway formed in the CO dissociation steps reacted with co-adsorbed hydrogen or carbon monoxide to produce H2O and CO2,respectively.The secondary pathway was the water-gas shift reaction.The results indicated that the CO2 production led to an increase in both primary and secondary pathways,and H2O production decreased when surface basicity of the catalyst increased in the order Ca 〉 Mg 〉 La. 展开更多
关键词 Ca promoter Mg promoter La promoter Fischer-Tropsch synthesis iron-based catalyst water-gas shift reaction
在线阅读 下载PDF
牙鲆GHR基因Promoter区微卫星序列多态性与生长性状关系的初步研究 被引量:10
10
作者 倪静 尤锋 +5 位作者 于深辉 张培军 徐冬冬 吴志昊 文爱韵 徐永立 《中国海洋大学学报(自然科学版)》 CAS CSCD 北大核心 2008年第5期719-725,共7页
采用牙鲆GHR基因5’端Promoter区的1个微卫星标记,对胶南和日照2个牙鲆养殖群体进行了群体遗传多样性的研究,并探索该基因多态性位点与牙鲆生长性状之间的相关性。结果表明,2个群体在该座位的等位基因数为12和9个,有效等位基因数为6.26... 采用牙鲆GHR基因5’端Promoter区的1个微卫星标记,对胶南和日照2个牙鲆养殖群体进行了群体遗传多样性的研究,并探索该基因多态性位点与牙鲆生长性状之间的相关性。结果表明,2个群体在该座位的等位基因数为12和9个,有效等位基因数为6.26和5.04个,多态信息含量为0.84和0.80。2个群体该座位的Hardy-Weinberg遗传偏离指数均为正值,并没有显示出杂合子缺失,但各基因型分布频率都在一定程度上偏离Hardy-Weinberg平衡(P<0.01)。连锁分析中发现,在胶南群体中,IM基因型对应的个体在全重、全长、体长、头长、体高和眼径形态学数据中均是最大的;在日照群体中,BC基因型对应的个体在全重、全长、体高、尾柄高、尾柄长和眼径数据中均是最大的;而CJ基因型对应的个体在体长和头长这两组数据中是最大的。该结果为研究GHR基因的变异对鱼类生长发育的影响及探索将该基因作为生长遗传标记的可行性奠定了实验基础。 展开更多
关键词 牙鲆 GHR基因5’端promoter区 微卫星 遗传多样性 性状连锁分析
在线阅读 下载PDF
Isolation and Analysis of Rubber Hevein Gene and Its Promoter Sequence 被引量:4
11
作者 邓晓东 费小雯 +1 位作者 黄俊生 郑学勤 《Acta Botanica Sinica》 CSCD 2002年第8期936-940,共5页
Hevein, a lectin_like protein, is a major factor of lutoids in the latex of rubber trees ( Hevea brasiliensis Muell._Arg.). This factor is involved in coagulation of the latex and has the ability to bind chitin. Th... Hevein, a lectin_like protein, is a major factor of lutoids in the latex of rubber trees ( Hevea brasiliensis Muell._Arg.). This factor is involved in coagulation of the latex and has the ability to bind chitin. The hevein gene with a length of 680 bp was cloned by the method of RT_PCR. Its promoter region with 1 306 bp of this gene was also isolated by genome walking, and its sequence included the typical TATA and CAAT boxes as well as the homologous sequence of abscisic acid (ABA) response elements. Expression of the hevein gene in the latex and leaves was detected by Northern blot. After treatment of the trees with ethylene and ABA, the results showed that the hevein gene was expressed principally in latex, and the expression could be induced by ethylene and ABA. 展开更多
关键词 rubber tree HEVEIN promoteR Northern blot
在线阅读 下载PDF
Isolation of a Genomic DNA for Gastrodia Antifungal Protein and Analyses of Its Promoter in Transgenic Tobacco 被引量:3
12
作者 萨其拉 王义琴 +2 位作者 李文彬 张利明 孙勇如 《Acta Botanica Sinica》 CSCD 2003年第2期229-233,共5页
A genomic DNA containing 5'-upstream region and complete open reading frame of a Gastrodia antifungal protein was isolated by screening of a genomic library from Gastrodia elata B1. To investigate the promoter act... A genomic DNA containing 5'-upstream region and complete open reading frame of a Gastrodia antifungal protein was isolated by screening of a genomic library from Gastrodia elata B1. To investigate the promoter activity, the 5'-flanking region - 1 157 lip upstream from the putative transcription start site was fused to the coding sequence of beta-glucuronidase (GUS) gene and transformed into Nicotiana tabacum. The strongest GUS activity was detected in the roots of transgenic tobacco, followed by stems. The leaves only showed a low GUS activity. Furthermore, the promoter established inducible expression pattern in transgenic tobacco upon fungus Trichoderma viride inoculation and jasmonic acid and salicylic acid treatments. 展开更多
关键词 Gastrodia elata Bl. promoteR transgenic tobacco organ-specific induced expression
在线阅读 下载PDF
Study on Cloning and Polymorphism of Porcine Adiponectin Promoter 被引量:2
13
作者 凌飞 汪亮亮 +2 位作者 杜红丽 李仕新 陈瑶生 《Agricultural Science & Technology》 CAS 2009年第6期53-56,共4页
[ Objective] The aim of this study was to provide a basis for study on adiponectin as a candidate gene for fat deposition. [ Method] The promoter sequence of adiponectin was obtained by porcine BAC library screening a... [ Objective] The aim of this study was to provide a basis for study on adiponectin as a candidate gene for fat deposition. [ Method] The promoter sequence of adiponectin was obtained by porcine BAC library screening and primer-walking method. The polymorphisms of adiponectin promoter from 290 pigs, including 5 breeds of Lantang pig, Large spotted pig, Large white pig, Landrace and Duroc, were analyzed with PCR-RFLP. [ Result] At SNP site of adiponectin 5'-flanking region -1 010 bp (G/A), GG genotype frequency in Chinese indigenous pigs was significantly higher than that in exotic pigs. At SNP site of adiponectin 5'-flanking region -394 bp (T/C), the genotype distribution of Chi- nese indigenous pigs was abundant, while no CC genotype was detected in exotic pigs, and T allele frequency was higher in exotic pigs. [ Conclusion] SNP site mutation of - 1 010 bp (G/A) may lead to changes of the gene transcription level, while SNP site of -394 bp (T/C) properly has no relationship with gene transcription level and fat deposition. 展开更多
关键词 PORCINE BAC library ADIPONECTIN promoteR SNP
在线阅读 下载PDF
Obtaining High Pest_resistant Transgenic Upland Cotton Cultivars Carrying cry1Ac3 Gene Driven by Chimeric OM Promoter 被引量:5
14
作者 陈宛新 肖桂芳 朱祯 《Acta Botanica Sinica》 CSCD 2002年第8期963-970,共8页
Hypocotyl segments from aseptic seedlings of two important cultivars of upland cotton ( Gossypium hirsutum L.) in Northwest China, 'Xinluzao_1', 'Jinmian_7', 'Jinmian_12' and 'Jihe_321&#... Hypocotyl segments from aseptic seedlings of two important cultivars of upland cotton ( Gossypium hirsutum L.) in Northwest China, 'Xinluzao_1', 'Jinmian_7', 'Jinmian_12' and 'Jihe_321' were transformed respectively by two efficient plant expression plasmids pBinMoBc and pBinoBc via Agrobacterium tumefaciens . In pBinMoBc, cry 1Ac3 gene, which encodes the Bt toxin, is under the control of chimeric OM promoter. In pBinoBc, it is under control of CaMV 35S promoter. After co_cultivation with Agrobacterium tumefimpfaciens LBA4404 (containing pBinMoBc or pBinoBc), kanamycin_resistant selection, somatic embryos were induced and regenerated plants were obtained. Then the regenerated plantlets were grafted to untransformed stocks in greenhouse to produce descendants. The integration of cry 1Ac3 gene and its expression in T 2 generation of transgenic cotton plants were confirmed by Southern hybridization and Western blotting. The analyses of insect bioassay indicated that the transgenic plants of both constructions have significant resistance to the larvae of cotton bollworm ( Heliothis armigera ) and that cry 1Ac3 gene driven by chimeric OM promoter could endue T 2 generation cotton with high pest_resistant ability, implicating that it has a profound application in genetic engineering to breed new pest_resistant cotton varieties. 展开更多
关键词 upland cotton insect_resistant cotton transgenic plant Agrobacterium tumefaciens cry 1Ac3 gene chimeric promoter
在线阅读 下载PDF
Effects of Supports and Promoters on in situ Hydrogenation of o-Cresol over Ni-based Catalysts 被引量:1
15
作者 李雁斌 徐莹 +3 位作者 马隆龙 王铁军 张琦 陈冠益 《Chinese Journal of Chemical Physics》 SCIE CAS CSCD 2014年第6期697-703,I0004,共8页
We investigated the effects of supports (CMK-3, SiO2ZrO2, MgO, Al2O3) and promoters (Cu, Ce, Fe) on textual properties of Ni based catalysts. o-Cresol was used as a probe to test the activity of these catalysts un... We investigated the effects of supports (CMK-3, SiO2ZrO2, MgO, Al2O3) and promoters (Cu, Ce, Fe) on textual properties of Ni based catalysts. o-Cresol was used as a probe to test the activity of these catalysts under the condition of 230 ℃ and nitrogen pres-sure of 0.1 MPa. The catalysts were characterized by X-ray diffraction, H2 temperature programmed reduction ammonium programmed desorption, and N2 adsorption-desorption isotherms. The results showed that the catalytic performance of Ni/CMK-3 (the conversion of o-cresol reached 45.4%) was significantly better than the other three kinds of supports. The modification of Ni/CMK-3 was also investigated and over 60% conversion of o-cresol was obtained after the addition of Ce (64.6%)and Cu (66.8%) in Ni/CMK-3, whereas the addition of Fe led to a decrease of conversion. In the meantime, Cu changed the products dis-tribution. The appearance of toluene indicated that another pathway existed in the reaction.Accompanied by the ascension of conversion in both sides, side effects also occurred and got more serious. The apparent order of activity for all the tested catalysts was NiCe/CMK-3〉NiCu/CMK-3〉Ni/CMK-3〉NiFe/CMK-3〉Ni/Al2O3Ni/SiO2ZrO2〉Ni/MgO. The reac-tion pathway, involving three routes, was also mentioned in this study. 展开更多
关键词 in-situ hydrogenation SUPPORT promoteR O-CRESOL
在线阅读 下载PDF
Isolation and Characterization of CMO Gene Promoter from Halophyte Suaeda liaotungensis K. 被引量:1
16
作者 李秋莉 尹辉 +3 位作者 李丹 朱宏飞 张毅 朱巍巍 《Journal of Genetics and Genomics》 SCIE CAS CSCD 北大核心 2007年第4期355-361,共7页
The 5'-flanking proximal region of stress-induced gene encoding choline monooxygenase (CMO) was isolated by Adaptor-PCR and TAIL-PCR from halophyte Suaeda liaotungensis K. A total of 2,204 bp DNA sequence was obtai... The 5'-flanking proximal region of stress-induced gene encoding choline monooxygenase (CMO) was isolated by Adaptor-PCR and TAIL-PCR from halophyte Suaeda liaotungensis K. A total of 2,204 bp DNA sequence was obtained. The transcription start site, which is located at 128 bp upstream to the start ATG, was predicted by the TSSP-TCM program. The functional elements were analysed by PLACE program. The obtained SICMO gene promoter contains the basic elements: TATA-box, CAAT-box, and stress-induced elements, for example, salt responsive element (GAAAAA), cold responsive elements (CANNTG), ABA (Abscisic Acid) responsive elements (NAACAA), water stress element (CGGTTG), and WUN responsive elements (GTTAGGTTC). Isolation and analysis of the promoter of the CMO gene from S. liaotungensis lays a foundation for characterising the stress-induced promoter elements, studying the relationship between the structure and function of the promoter, and investigating the molecular mechanism of CMO gene regulation. 展开更多
关键词 Suaeda liaotungensis K. choline monooxygenase (CMO) promoteR
在线阅读 下载PDF
Cloning of the Promoter Regions of Golgi Membrane Glycoprotein GP73 被引量:2
17
作者 谢红彬 宫钰 彭涛 《Agricultural Science & Technology》 CAS 2008年第3期39-42,136,共5页
[Objective] The aim of this study is to clone the active promoter of golgi membrane glycoprotein GP73. [Method] The sequence within the range of upstream 1 000 bp and downstream 400 bp of transcription initiation site... [Objective] The aim of this study is to clone the active promoter of golgi membrane glycoprotein GP73. [Method] The sequence within the range of upstream 1 000 bp and downstream 400 bp of transcription initiation site was analyzed, the genomic DNA of hepatoma cell line Huh-7 was regarded as template for PCR amplification. The amplified fragment was cloned into recombinant construct with enhanced green fluorescent protein (EGFP) report gene. The expression of EGFP in recombinants were observed under fluorescence microscope after transfection, with the assistant of flow cytometry. [Result] The 1 310 bp ranging between upstream 980 bp and downstream 330 bp of transcription initiation site assumed promoter function. The region contains two core promoters and several conserved sequences including TATA box and many DNA binding sites such as NF-κB, AP1, GC-SP1. [Conclusion] The cloning of the promoter provides a reference for the study of the transcription mechanism of GP73. 展开更多
关键词 GP73 promoteR SEQUENCE ANALYSIS
在线阅读 下载PDF
Expression Activity of CLCuV Bidirectional Promoter in Agrobacterium tumefaciens 被引量:1
18
作者 谢迎秋 孟蒙 +3 位作者 徐鸿林 吴茜 陈蕾 朱祯 《Acta Botanica Sinica》 CSCD 2001年第10期1052-1054,共3页
Cotton leaf curl virus (CLCuV) belongs to the subgroup III of geminiviruses with single strand DNA genome. Study demonstrated that the bidirectional promoter of CLCuV had activity in Agrobacterium tumefaciens (Smith e... Cotton leaf curl virus (CLCuV) belongs to the subgroup III of geminiviruses with single strand DNA genome. Study demonstrated that the bidirectional promoter of CLCuV had activity in Agrobacterium tumefaciens (Smith et Townsend) Conn. This is the first report for the activity of the bidirectional promoter of geminivirus in A. tumefaciens. Results showed that the activity of the complementary sense promoter was stronger than that of virion sense promoter, and was detected 2-fold higher than that of CaMV 35S promoter in A. tumefaciens. Moreover, the promoter 5' deletion analysis indicated that the mean GUS activity driven by a 287 nucleotides complementary sense promoter fragment (from-287 to the translation initiation site) is 4 times higher than that driven by the whole complementary sense promoter in A. tumefaciens. This result suggested that there might exist negative regulatory elements in this deleted fragment. The function of other cis-elements included in CLCuV complementary sense promoter was also discussed in this paper. 展开更多
关键词 GUS cotton-leaf curl virus (CLCuV) promoteR CIS-ELEMENT
在线阅读 下载PDF
Cloning and Functional Analysis of the Porcine Growth Hormone Gene Promoter 被引量:1
19
作者 阮楠 张明军 +2 位作者 鞠辉明 白立景 赵为民 《Agricultural Science & Technology》 CAS 2012年第4期893-896,共4页
[Objective] This study aimed to clone the porcine growth hormone gene promoter and determine the core promoter sequences and the cis-acting elements. [Method] Sequence of the 5'flanking region of porcine growth hormo... [Objective] This study aimed to clone the porcine growth hormone gene promoter and determine the core promoter sequences and the cis-acting elements. [Method] Sequence of the 5'flanking region of porcine growth hormone gene was searched out and downloaded from the NCBI website. According to the targeted se- quence, primers were designed and synthesized for the PCR amplification. The 1 882 bp (-1 821 bp-+61 bp) fragment was amplified by PCR. Nine promoter frag- ments with different lengths were obtained by genome-walking deletion method and then cloned into luciferase reporter vectors. Relative transcriptional activities of these 5' terminal-deleted plasmids in pituitary and non-pituitary cells were determined by transient transfection of the rat pituitary adenoma cell (GH3), porcine lilac endotheli- um cell (PIEC) and porcrne Kidney-15 (PK15) with the constructed dual-luciferase vectors. [Result] Result of DNA sequencing showed that the 1 882 bp fragment of GH 5' promoter was successfully cloned. Nine luciferase reporter gene plasmids were constructed. DuaI-Luciferase reporter assay indicated that the promoter inserted into reporter gene vector had very strong cell specificity. [Conclusion] Porcine growth hormone gene specifically expresses in pituitary cells. The minimal promoter of the porcine growth hormone gene is mapped at the region -110 bp-+61 bp. Promoter regions 218 bp--110 bp and -429 bp--218 bp contain positive regulatory elements. 展开更多
关键词 Porcine growth hormone gene promoter Gene expression REGULATION
在线阅读 下载PDF
Low-dose natural clay Kaolin promotes the growth of submerged macrophytes and alters the rhizosphere microorganism community:Implications for lake restoration
20
作者 Shangsheng Sun Qingchuan Chou +5 位作者 Qi Ding Yuqing Su Haojie Su Erik Jeppesen Liqing Wang Wei Zhang 《Journal of Environmental Sciences》 2025年第8期521-535,共15页
Sediment properties have a crucial effect on the growth and recovery of aquatic plants in lakes.Addition of various chemical substances has been proposed to reinforce the recovery of plants after a nutrient loading re... Sediment properties have a crucial effect on the growth and recovery of aquatic plants in lakes.Addition of various chemical substances has been proposed to reinforce the recovery of plants after a nutrient loading reduction.However,the effects of such sediment amendments on plant growth,especially those from rhizosphere microorganisms,is limited.We added Kaolin clay to sediments in different concentrations to explore its impact on the growth of Vallisneria natans and Ottelia acuminate and the concurrent shift in rhizosphere microorganisms using high-throughput sequencing technology.We found that the addition of low doses(10%and 20%in mass ratio)of Kaolin significantly modified sediment conditions(oxidation reduction potential and pH),with implications also for the composition,diversity,and stability of rhizosphere microorganisms.LEfSe analysis revealed that low-dose addition of Kaolin increased the abundances of functional microbial groups that benefit plant nutrient absorption and enhance plant stress resistance,such as Spirillaceae,Rhodocyclaceae,and Burkholderiales.Moreover,low doses of Kaolin significantly promoted the photosynthesis and nutrient absorption of submerged macrophytes,thereby facilitating plant growth.A structural equation model(SEM)indicated that the direct impact of Kaolin on the growth of submerged plants was relatively minor,while the indirect effect through modulation of rhizosphere microorganisms was important.Our study suggests that low doses of Kaolin may be used to promote the growth of submerged macrophytes when lakes with a high organic content in the sediment are recovering after nutrient loading reduction. 展开更多
关键词 KAOLIN Vallisneria natans Ottelia acuminata Rhizosphere microorganisms Growth promotion effect
原文传递
上一页 1 2 119 下一页 到第
使用帮助 返回顶部