The bioaccumulation and elimination rule of prometryn in hard clam Meretrix meretrix were studied under semistatic conditions. Whenthe water temperature was （12±1）℃, M. meretrix were exposed to the seawater co...The bioaccumulation and elimination rule of prometryn in hard clam Meretrix meretrix were studied under semistatic conditions. Whenthe water temperature was （12±1）℃, M. meretrix were exposed to the seawater containing 1, 10, and 100 μg/L prometryn. The content of prometrynin M. meretrix increased with the increase of exposed concentration, and both had significant positive correlation. The accumulation of prometryn inM. meretrix first increased then decreased, and finally fluctuated at a certain level. The maximum accumulation content was observed in M. meretrixwhen exposed to prometryn for 10, 14 and 18 d, and the maximum bioaccumulation factors （BCFs） were 22.9, 8.3 and 4.3, respectively. At the forthday of the elimination test, the content of prometryn in M. meretrix of each group were decreased above 50%. At the 22ndday of the elimination test,the content of prometryn in M. meretrix were decreased to 8.7%, 5.6% and 2.6% of the initial content in the elimination test, respectively. The results indicated that hard clam M. meretrix had strong bioaccumulation and elimination ability on prometryn.展开更多
We report the development of a sensitive,fast,and simple method for the detection of prometryn(PRO)in potato and celery using a lateral flow immunochromatographic assay(LFIA).A monoclonal antibody(mAb)against PRO was ...We report the development of a sensitive,fast,and simple method for the detection of prometryn(PRO)in potato and celery using a lateral flow immunochromatographic assay(LFIA).A monoclonal antibody(mAb)against PRO was prepared under optimized conditions,and the 50%inhibition concentration(IC50)for use in ELISA was determined to be 0.6 ng mL−1.Moreover,the cut-off value for use in an LFIA was 50 ng mL−1.To validate the reliability of the developed LFIA strip,real samples were used,and the recovery rate was found to be 99.3-104.3%.The assays were completed within 8 min,demonstrating the suitability of our developed methods for the detection of PRO in potato and celery.展开更多
利用溶胶-凝胶法制备出PEG-20M的固相微萃取头(SPME Head fiber),将自制的微萃取装置与气相色谱(GC)联用,用于蔬菜样品中残留扑草净的分析。固相微萃取条件主要讨论了萃取时间和搅拌条件的影响,解析条件主要考虑了解析温度、解析时间等...利用溶胶-凝胶法制备出PEG-20M的固相微萃取头(SPME Head fiber),将自制的微萃取装置与气相色谱(GC)联用,用于蔬菜样品中残留扑草净的分析。固相微萃取条件主要讨论了萃取时间和搅拌条件的影响,解析条件主要考虑了解析温度、解析时间等因素,通过实验获得了优化的固相微萃取和SPME/GC的主要操作参数。以扑草净为分析对象,以自制的PEG-20M萃取装置分离富集蔬菜中残留扑草净,结果表明PEG-20M萃取头对三嗪类农药扑草净具有较好的分离富集作用。展开更多
基金Supported by Science and Technology Plan of Nanjing Customs(Former Jiangsu Inspection and Quarantine Bureau)(2017KJ12)
文摘The bioaccumulation and elimination rule of prometryn in hard clam Meretrix meretrix were studied under semistatic conditions. Whenthe water temperature was （12±1）℃, M. meretrix were exposed to the seawater containing 1, 10, and 100 μg/L prometryn. The content of prometrynin M. meretrix increased with the increase of exposed concentration, and both had significant positive correlation. The accumulation of prometryn inM. meretrix first increased then decreased, and finally fluctuated at a certain level. The maximum accumulation content was observed in M. meretrixwhen exposed to prometryn for 10, 14 and 18 d, and the maximum bioaccumulation factors （BCFs） were 22.9, 8.3 and 4.3, respectively. At the forthday of the elimination test, the content of prometryn in M. meretrix of each group were decreased above 50%. At the 22ndday of the elimination test,the content of prometryn in M. meretrix were decreased to 8.7%, 5.6% and 2.6% of the initial content in the elimination test, respectively. The results indicated that hard clam M. meretrix had strong bioaccumulation and elimination ability on prometryn.
基金supported by the National Natural Science Foundation of China(22236002).
文摘We report the development of a sensitive,fast,and simple method for the detection of prometryn(PRO)in potato and celery using a lateral flow immunochromatographic assay(LFIA).A monoclonal antibody(mAb)against PRO was prepared under optimized conditions,and the 50%inhibition concentration(IC50)for use in ELISA was determined to be 0.6 ng mL−1.Moreover,the cut-off value for use in an LFIA was 50 ng mL−1.To validate the reliability of the developed LFIA strip,real samples were used,and the recovery rate was found to be 99.3-104.3%.The assays were completed within 8 min,demonstrating the suitability of our developed methods for the detection of PRO in potato and celery.
文摘利用溶胶-凝胶法制备出PEG-20M的固相微萃取头(SPME Head fiber),将自制的微萃取装置与气相色谱(GC)联用,用于蔬菜样品中残留扑草净的分析。固相微萃取条件主要讨论了萃取时间和搅拌条件的影响,解析条件主要考虑了解析温度、解析时间等因素,通过实验获得了优化的固相微萃取和SPME/GC的主要操作参数。以扑草净为分析对象,以自制的PEG-20M萃取装置分离富集蔬菜中残留扑草净,结果表明PEG-20M萃取头对三嗪类农药扑草净具有较好的分离富集作用。
