Pollution from road traffic contributes significantly to air pollution through pollutants from exhaust emissions(gases and particles)and non-exhaust emissions(tire wear particles,brake wear particles and the resuspens...Pollution from road traffic contributes significantly to air pollution through pollutants from exhaust emissions(gases and particles)and non-exhaust emissions(tire wear particles,brake wear particles and the resuspension of road dust).This research examined the hazard of tire particles(TP)and in particular evaluated the effect of TP size on lung macrophages.TP were obtained by cryogenic grinding of a tire and subsequent sieving to obtain four groups of particles(TP70,TP30,TP15,TP5)of different sizes with average diameters of 107μm,55μm,22μm,and 6μm,respectively.A complete physicochemical characterization was performed to determine the size distribution,chemical composition and morphology of these particles.We then investigated the proinflammatory response,oxidative stress and cytotoxicity induced in RAW264.7 cells exposed to four different TP concentrations for 24 h.TP had no direct effect on cytotoxicity,nor did they increase reactive oxygen species(ROS)production in the cells.However,TP induced a significant and size-dependent proinflammatory effect,which was particularly pronounced with small particles.Moreover,this effect was concentration-dependent.展开更多
Ammonia,a common toxic gas,is not only one of the main causes of haze,but also can enter respiratory tract and directly affect the health of humans and animals.Pig was used as an animal model for exploring the molecul...Ammonia,a common toxic gas,is not only one of the main causes of haze,but also can enter respiratory tract and directly affect the health of humans and animals.Pig was used as an animal model for exploring the molecular mechanism and dose effect of ammonia toxicity to lung.In this study,the apoptosis of type II alveolar epithelial cells was observed in high ammonia exposure group using transmission electron microscopy.Gene and protein expression analysis using transcriptome sequencing and western blot showed that low ammonia exposure induced T-cell-involved proinflammatory response,but high ammonia exposure repressed the expression of DNA repair-related genes and affected ion transport.Moreover,high ammonia exposure significantly increased 8-hydroxy-2-deoxyguanosine (8-OHdG) level,meaning DNA oxidative damage occurred.In addition,both low and high ammonia exposure caused oxidative stress in pig lungs.Integrated analysis of transcriptome and metabolome revealed that the up-regulation of LDHB and ND2 took part in high ammonia exposure-affected pyruvate metabolism and oxidative phosphorylation progress,respectively.Inclusion,oxidative stress mediated ammonia-induced proinflammatory response and apoptosis of porcine lungs.These findings may provide new insights for understanding the ammonia toxicity to workers in livestock farms and chemical fertilizer plants.展开更多
Emodin[1,3,8-Trihydroxy-6-methylanthraquinone]has been reported to exhibit vascular anti-inflammatory properties.However,the relevant anti-inflammatory mechanisms are not well understood.The present study was design t...Emodin[1,3,8-Trihydroxy-6-methylanthraquinone]has been reported to exhibit vascular anti-inflammatory properties.However,the relevant anti-inflammatory mechanisms are not well understood.The present study was design to explore the molecular target(s)of emodin in modifying lipopolysaccharide(LPS)-associated signal transduction pathway in human umbilical vein endothelial cells(HUVECs).Cultured HUVECs were pre-incubated with 1 to 50μg/ml emodin for 30 min,LPS-induced proinflammatory cytokines(IL-1,IL-6)and chemokines(IL-8,MCP-1)expression were inhibited dose-dependently,which agreed well with the NF-B activation and IB degradation detected by immunocytochemistry and western blotting,respectively.展开更多
L.Zhang et al.proposed novel immunotherapeutic strategies to limit the progression of inflammatory bowel diseases by targeting the interplay between the membrane-bound CD39 molecule and cellular metabolism—specifical...L.Zhang et al.proposed novel immunotherapeutic strategies to limit the progression of inflammatory bowel diseases by targeting the interplay between the membrane-bound CD39 molecule and cellular metabolism—specifically,by increasing regulatory T-cell function through the modulation of glucose metabolism and suppressing pathogenic proinflammatory Th17 responses via the modulation of oxidative metabolism.展开更多
基金supported by the Ecole des Mines Saint-Etienne and the Agence de l’Environnement et de la Maitrise de l’Energie。
文摘Pollution from road traffic contributes significantly to air pollution through pollutants from exhaust emissions(gases and particles)and non-exhaust emissions(tire wear particles,brake wear particles and the resuspension of road dust).This research examined the hazard of tire particles(TP)and in particular evaluated the effect of TP size on lung macrophages.TP were obtained by cryogenic grinding of a tire and subsequent sieving to obtain four groups of particles(TP70,TP30,TP15,TP5)of different sizes with average diameters of 107μm,55μm,22μm,and 6μm,respectively.A complete physicochemical characterization was performed to determine the size distribution,chemical composition and morphology of these particles.We then investigated the proinflammatory response,oxidative stress and cytotoxicity induced in RAW264.7 cells exposed to four different TP concentrations for 24 h.TP had no direct effect on cytotoxicity,nor did they increase reactive oxygen species(ROS)production in the cells.However,TP induced a significant and size-dependent proinflammatory effect,which was particularly pronounced with small particles.Moreover,this effect was concentration-dependent.
基金supported by the National Natural Science Foundation of China (No.31872402)。
文摘Ammonia,a common toxic gas,is not only one of the main causes of haze,but also can enter respiratory tract and directly affect the health of humans and animals.Pig was used as an animal model for exploring the molecular mechanism and dose effect of ammonia toxicity to lung.In this study,the apoptosis of type II alveolar epithelial cells was observed in high ammonia exposure group using transmission electron microscopy.Gene and protein expression analysis using transcriptome sequencing and western blot showed that low ammonia exposure induced T-cell-involved proinflammatory response,but high ammonia exposure repressed the expression of DNA repair-related genes and affected ion transport.Moreover,high ammonia exposure significantly increased 8-hydroxy-2-deoxyguanosine (8-OHdG) level,meaning DNA oxidative damage occurred.In addition,both low and high ammonia exposure caused oxidative stress in pig lungs.Integrated analysis of transcriptome and metabolome revealed that the up-regulation of LDHB and ND2 took part in high ammonia exposure-affected pyruvate metabolism and oxidative phosphorylation progress,respectively.Inclusion,oxidative stress mediated ammonia-induced proinflammatory response and apoptosis of porcine lungs.These findings may provide new insights for understanding the ammonia toxicity to workers in livestock farms and chemical fertilizer plants.
基金supported by National Natural Science Foundation of China,30700151Youth Investigator Fund from UESTC,Y02018023601062
文摘Emodin[1,3,8-Trihydroxy-6-methylanthraquinone]has been reported to exhibit vascular anti-inflammatory properties.However,the relevant anti-inflammatory mechanisms are not well understood.The present study was design to explore the molecular target(s)of emodin in modifying lipopolysaccharide(LPS)-associated signal transduction pathway in human umbilical vein endothelial cells(HUVECs).Cultured HUVECs were pre-incubated with 1 to 50μg/ml emodin for 30 min,LPS-induced proinflammatory cytokines(IL-1,IL-6)and chemokines(IL-8,MCP-1)expression were inhibited dose-dependently,which agreed well with the NF-B activation and IB degradation detected by immunocytochemistry and western blotting,respectively.
基金supported by the CRUK Hepatocellular Carcinoma Expediter Network(HUNTER)Accelerator Award 2018(Project 620 Id.122794)(VB)Associazione Italiana per la Ricerca sul Cancro(AIRC)IGV19939(VB)+2 种基金Fondazione Italiana Sclerosi Multipla(FISM)onlus(cod.2015/RVsingle/04 and 2019/RVsingle/053)(VB)AIRC-IG 2023(ID 29244)(SS),Ministero della Salute(PNRR-MAD-2022-12375947)(SS)the European Union-Next Generation EU in the context of the National Recovery and Resilience Plan,Investment Partenariato Esteso PE8“Conseguenze e sfide dell’invecchiamento”,Project Age-It(Aging Well in an Aging Society)(SS).
文摘L.Zhang et al.proposed novel immunotherapeutic strategies to limit the progression of inflammatory bowel diseases by targeting the interplay between the membrane-bound CD39 molecule and cellular metabolism—specifically,by increasing regulatory T-cell function through the modulation of glucose metabolism and suppressing pathogenic proinflammatory Th17 responses via the modulation of oxidative metabolism.
