This study aims to investigate the protective effects of peroxiredoxin 6 on the total motility and progressive motility of human spermatozoa.Semen samples with normal parameters were collected from 23 males and supple...This study aims to investigate the protective effects of peroxiredoxin 6 on the total motility and progressive motility of human spermatozoa.Semen samples with normal parameters were collected from 23 males and supplemented with different concentrations of peroxiredoxin 6.All the semen samples were measured according to the WHO 5th manual,and the motile spermatozoa were extracted using IVF fertilization medium supplemented with different peroxiredoxin 6 concentrations.Total motility and progressive motility were observed at different time-points of culture at room temperature.After peroxiredoxin 6 supplementation,all groups had a significant increase in total motility and progressive motility compared to the control group.The difference in total motility and progressive motility between the 0 and 10−7 mM groups was observed at 24 and 48 h of culture at room temperature.At 24 h,the total motility increased by 30%in the control group(16.03±11.91 vs.11.51±7.84),and progressive motility increased by 21%(10.53±9.4 vs.8.31±6.04).A similar trend was observed in the 48 h group.In addition,we also found that peroxiredoxin 6 had a well protective effect on sperm kinetic parameters at 10−7 mM.The findings of this study suggest that peroxiredoxin 6 can enhance sperm total motility and progressive motility in IVF fertilization medium.Peroxiredoxin 6 may have potential benefits for sperm preparation in assisted reproductive technology.展开更多
This study evaluated the relationship between serum L-carnitine level and sperm parameters in young boars. Serum L-carnitine and semen characteristics were determined for 61 young Duroc boars between the ages of 590 a...This study evaluated the relationship between serum L-carnitine level and sperm parameters in young boars. Serum L-carnitine and semen characteristics were determined for 61 young Duroc boars between the ages of 590 and 630 days. Multiple linear regression analysis was performed to predict total and progressive motility and the total number of spermatozoa based on serum total L-carnitine and free L-carnitine levels. Total number of spermatozoa was not associated with basal serum L-carnitine levels. A regression equation was found in which both total L-carnitine levels and free L-carnitine levels were significant predictors of total and progressive motility (P 0.05). These results suggest that serum L-carnitine level is an important selection parameter for stock boars.展开更多
This study aimed to investigate the associations between the post-wash total progressively motile sperm count(TPMSC)in the first intrauterine insemination(IUI)cycle and pregnancy outcomes of the second IUI cycle.Data ...This study aimed to investigate the associations between the post-wash total progressively motile sperm count(TPMSC)in the first intrauterine insemination(IUI)cycle and pregnancy outcomes of the second IUI cycle.Data were retrieved from the clinical database at the Reproductive Center of Peking University Third Hospital(Beijing,China)between January 2011 and December 2022.Couples were included in this retrospective cohort study if they had unexplained or mild male factor infertility and were treated with IUI for two consecutive cycles using the same protocol.A total of 8290 couples were included in the analysis.The mean±standard deviation(s.d.)age of women was 32.0±3.5 years.We categorized groups based on the post-wash TPMSC(×10^(6))levels in the first IUI cycle:group 1(0<TPMSC<1,n=1290),group 2(1≤TPMSC<2,n=863),group 3(2≤TPMSC<3,n=800),group 4(3≤TPMSC<4,n=783),group 5(4≤TPMSC<5,n=1541),group 6(5≤TPMSC<6,n=522),group 7(6≤TPMSC<7,n=547),group 8(7≤TPMSC<8,n=175),group 9(8≤TPMSC<9,n=556),group 10(9≤TPMSC<10,n=192),and group 11(TPMSC≥10,n=1021).The primary outcome was live birth rate of the second IUI cycle.Live birth rates were 7.9%,5.8%,7.6%,7.4%,7.3%,8.4%,7.5%,7.4%,8.8%,8.9%,and 7.6%in each group,respectively.There were no statistically significant differences in clinical pregnancy rates or live birth rates between any groups and those with the post-wash TPMSC<1×10^(6).In an IUI program for unexplained and mild male factor infertility,the post-wash TPMSC in the first IUI cycle was not significantly associated with the live birth rate in the second IUI cycle.展开更多
Objective:To evaluate the impact of seasonal and geographical temperature on sperm parameters in Indian men who were partners in subfertile couples.Methods:A retrospective review of the records of semen analysis from ...Objective:To evaluate the impact of seasonal and geographical temperature on sperm parameters in Indian men who were partners in subfertile couples.Methods:A retrospective review of the records of semen analysis from January 2009 to December 2017 that was performed as part of primary evaluation of infertile couple was undertaken.Mean values of various semen parameters were compared between seasons using one-way analysis of variance test followed by Bonferroni pair wise post-hoc comparison test.Bivariate Pearson correlation coefficient was applied to measure the correlation between age,sperm parameters and annual temperature/seasons.The logistic regression analysis was further carried out to identify significant variables contributing to normal progressive motility of sperm.Results:A total of 3433 reports of semen samples were analysed.There was no significant seasonal variation in the mean volume,concentration,and total motility levels(P>0.05)except the mean values of progressive motility(P<0.001).The mean values of progressive motility during pre-monsoon were significantly higher compared to the corresponding values during monsoon and autumn seasons(P<0.01).In all the seasons,total motility had significant positive correlation with sperm concentration and progressive motility(P<0.05).Pre-monsoon emerged as the significant favourable season to get normal progressive motility level(OR 1.32,95%CI 1.04-1.68)compared to winter.The chances of getting normal progressive motility reduced due to longer abstinence period(OR 0.87,95%CI 0.80-0.93).When the temperature was more than 28℃,the likelihood of getting normal progressive motility decreased(OR 0.70,95%CI 0.51-0.95).Conclusions:The progressive motility of sperms significantly decreases from pre-monsoon to autumn with recovery towards winter,which substantiates the effect of rising temperature on the progressive motility of sperm.This is clinically important to counsel infertile patients,so that they can plan their treatment accordingly.展开更多
According to JJF1059.2-2012 and CNAS-GL06, the measurement uncertainty of four indicators （semen volume, sperm motility and validity, progressive motility, abnormal sperm percentage） of boar fresh semen, as well as ...According to JJF1059.2-2012 and CNAS-GL06, the measurement uncertainty of four indicators （semen volume, sperm motility and validity, progressive motility, abnormal sperm percentage） of boar fresh semen, as well as its influencing factors, was analyzed. The results showed that the measurement uncertainty of semen volume and progressive motility caused by inspectors was low, and their measurement results reliability was high. There were small differences in measurement results of semen volume and progressive motility among inspectors. However, the measurement uncertainty of sperm motility and abnormal sperm percentage was relatively large, and great differences were found among different inspectors. Therefore, the training of inspectors＇ measuring sperm motility and abnormal sperm percentage should be strengthened, and the comparison of measurement results among inspectors should be performed in the future.展开更多
[Objectives]Straw-preserved frozen semen from native and imported pig breeds and its conception performance were studied to provide a scientific basis for further optimizing pig breeding strategy.[Methods]This study w...[Objectives]Straw-preserved frozen semen from native and imported pig breeds and its conception performance were studied to provide a scientific basis for further optimizing pig breeding strategy.[Methods]This study was conducted to systematically evaluate straw-preserved frozen semen produced by the Guizhou Testing Center for Livestock and Poultry Germplasm from 2018 to 2023.We analyzed 206 samples from native Guizhou pig breeds,including Kele,Zongdi Hua,and Nuogu pigs,as well as 363 samples from imported and bred pig breeds,such as Duroc,Large Yorkshire,Landrace,Topigs,and French Yorkshire,among others.Additionally,we assessed the conception performance of more than 300 heads across 10 batches,comprising both native and imported and bred pig breeds,following deep insemination.[Results]Significant differences were identified in sperm motility and abnormal sperm percentage(ABSP)among native breeds(P<0.05),as well as in number of progressively motile sperm(NPMS)and ABSP among imported and bred breeds(P<0.05).The average semen dose for both native and imported/bred pigs was 0.47 ml,with a standard deviation of 0.01 ml.Sperm motility ranged from 41.5%to 58%in native breeds and 50.4%to 61.7%in imported and bred breeds.The NPMS ranged from 144 to 177 million in native breeds and 149 to 177 million in imported and bred breeds.ABSPs remained within acceptable limits,ranging from 6.6%to 16.8%for native breeds and 6.1%to 18.1%for imported and bred breeds.The imported and bred pigs outperformed the native breeds in terms of sperm motility(50.42%to 69.58%vs.41.63%to 48.37%),farrowing rate(71.13%to 86.70%vs.57.15%to 74.35%),and litter size(7.74 to 10.30 vs.6.39 to 7.45).[Conclusions]These findings suggest that long-term selective breeding and domestication of native Guizhou pigs are necessary to enhance their reproductive performance.展开更多
基金supported by the Peking Post-doctoral Research Fund(EE2019-50)and Peking University International Hospital Research Funds(No.YN2019QN13).
文摘This study aims to investigate the protective effects of peroxiredoxin 6 on the total motility and progressive motility of human spermatozoa.Semen samples with normal parameters were collected from 23 males and supplemented with different concentrations of peroxiredoxin 6.All the semen samples were measured according to the WHO 5th manual,and the motile spermatozoa were extracted using IVF fertilization medium supplemented with different peroxiredoxin 6 concentrations.Total motility and progressive motility were observed at different time-points of culture at room temperature.After peroxiredoxin 6 supplementation,all groups had a significant increase in total motility and progressive motility compared to the control group.The difference in total motility and progressive motility between the 0 and 10−7 mM groups was observed at 24 and 48 h of culture at room temperature.At 24 h,the total motility increased by 30%in the control group(16.03±11.91 vs.11.51±7.84),and progressive motility increased by 21%(10.53±9.4 vs.8.31±6.04).A similar trend was observed in the 48 h group.In addition,we also found that peroxiredoxin 6 had a well protective effect on sperm kinetic parameters at 10−7 mM.The findings of this study suggest that peroxiredoxin 6 can enhance sperm total motility and progressive motility in IVF fertilization medium.Peroxiredoxin 6 may have potential benefits for sperm preparation in assisted reproductive technology.
文摘This study evaluated the relationship between serum L-carnitine level and sperm parameters in young boars. Serum L-carnitine and semen characteristics were determined for 61 young Duroc boars between the ages of 590 and 630 days. Multiple linear regression analysis was performed to predict total and progressive motility and the total number of spermatozoa based on serum total L-carnitine and free L-carnitine levels. Total number of spermatozoa was not associated with basal serum L-carnitine levels. A regression equation was found in which both total L-carnitine levels and free L-carnitine levels were significant predictors of total and progressive motility (P 0.05). These results suggest that serum L-carnitine level is an important selection parameter for stock boars.
基金supported by National Key Research and Development Program(2022YFC2705305)clinical key project of Peking University Third Hospital(BYSYZD2023007)National Science Fund for Distinguished Young Scholars(81925013).
文摘This study aimed to investigate the associations between the post-wash total progressively motile sperm count(TPMSC)in the first intrauterine insemination(IUI)cycle and pregnancy outcomes of the second IUI cycle.Data were retrieved from the clinical database at the Reproductive Center of Peking University Third Hospital(Beijing,China)between January 2011 and December 2022.Couples were included in this retrospective cohort study if they had unexplained or mild male factor infertility and were treated with IUI for two consecutive cycles using the same protocol.A total of 8290 couples were included in the analysis.The mean±standard deviation(s.d.)age of women was 32.0±3.5 years.We categorized groups based on the post-wash TPMSC(×10^(6))levels in the first IUI cycle:group 1(0<TPMSC<1,n=1290),group 2(1≤TPMSC<2,n=863),group 3(2≤TPMSC<3,n=800),group 4(3≤TPMSC<4,n=783),group 5(4≤TPMSC<5,n=1541),group 6(5≤TPMSC<6,n=522),group 7(6≤TPMSC<7,n=547),group 8(7≤TPMSC<8,n=175),group 9(8≤TPMSC<9,n=556),group 10(9≤TPMSC<10,n=192),and group 11(TPMSC≥10,n=1021).The primary outcome was live birth rate of the second IUI cycle.Live birth rates were 7.9%,5.8%,7.6%,7.4%,7.3%,8.4%,7.5%,7.4%,8.8%,8.9%,and 7.6%in each group,respectively.There were no statistically significant differences in clinical pregnancy rates or live birth rates between any groups and those with the post-wash TPMSC<1×10^(6).In an IUI program for unexplained and mild male factor infertility,the post-wash TPMSC in the first IUI cycle was not significantly associated with the live birth rate in the second IUI cycle.
文摘Objective:To evaluate the impact of seasonal and geographical temperature on sperm parameters in Indian men who were partners in subfertile couples.Methods:A retrospective review of the records of semen analysis from January 2009 to December 2017 that was performed as part of primary evaluation of infertile couple was undertaken.Mean values of various semen parameters were compared between seasons using one-way analysis of variance test followed by Bonferroni pair wise post-hoc comparison test.Bivariate Pearson correlation coefficient was applied to measure the correlation between age,sperm parameters and annual temperature/seasons.The logistic regression analysis was further carried out to identify significant variables contributing to normal progressive motility of sperm.Results:A total of 3433 reports of semen samples were analysed.There was no significant seasonal variation in the mean volume,concentration,and total motility levels(P>0.05)except the mean values of progressive motility(P<0.001).The mean values of progressive motility during pre-monsoon were significantly higher compared to the corresponding values during monsoon and autumn seasons(P<0.01).In all the seasons,total motility had significant positive correlation with sperm concentration and progressive motility(P<0.05).Pre-monsoon emerged as the significant favourable season to get normal progressive motility level(OR 1.32,95%CI 1.04-1.68)compared to winter.The chances of getting normal progressive motility reduced due to longer abstinence period(OR 0.87,95%CI 0.80-0.93).When the temperature was more than 28℃,the likelihood of getting normal progressive motility decreased(OR 0.70,95%CI 0.51-0.95).Conclusions:The progressive motility of sperms significantly decreases from pre-monsoon to autumn with recovery towards winter,which substantiates the effect of rising temperature on the progressive motility of sperm.This is clinically important to counsel infertile patients,so that they can plan their treatment accordingly.
文摘According to JJF1059.2-2012 and CNAS-GL06, the measurement uncertainty of four indicators （semen volume, sperm motility and validity, progressive motility, abnormal sperm percentage） of boar fresh semen, as well as its influencing factors, was analyzed. The results showed that the measurement uncertainty of semen volume and progressive motility caused by inspectors was low, and their measurement results reliability was high. There were small differences in measurement results of semen volume and progressive motility among inspectors. However, the measurement uncertainty of sperm motility and abnormal sperm percentage was relatively large, and great differences were found among different inspectors. Therefore, the training of inspectors＇ measuring sperm motility and abnormal sperm percentage should be strengthened, and the comparison of measurement results among inspectors should be performed in the future.
基金Supported by Guizhou Provincial Department of Agriculture and Rural Affairs Project(QNYZZZ[2017]No.12,GZSZCYJSTX-04)2024 Quality Supervision and Sampling Project of Normal Temperature Semen for Breeding Pigs.
文摘[Objectives]Straw-preserved frozen semen from native and imported pig breeds and its conception performance were studied to provide a scientific basis for further optimizing pig breeding strategy.[Methods]This study was conducted to systematically evaluate straw-preserved frozen semen produced by the Guizhou Testing Center for Livestock and Poultry Germplasm from 2018 to 2023.We analyzed 206 samples from native Guizhou pig breeds,including Kele,Zongdi Hua,and Nuogu pigs,as well as 363 samples from imported and bred pig breeds,such as Duroc,Large Yorkshire,Landrace,Topigs,and French Yorkshire,among others.Additionally,we assessed the conception performance of more than 300 heads across 10 batches,comprising both native and imported and bred pig breeds,following deep insemination.[Results]Significant differences were identified in sperm motility and abnormal sperm percentage(ABSP)among native breeds(P<0.05),as well as in number of progressively motile sperm(NPMS)and ABSP among imported and bred breeds(P<0.05).The average semen dose for both native and imported/bred pigs was 0.47 ml,with a standard deviation of 0.01 ml.Sperm motility ranged from 41.5%to 58%in native breeds and 50.4%to 61.7%in imported and bred breeds.The NPMS ranged from 144 to 177 million in native breeds and 149 to 177 million in imported and bred breeds.ABSPs remained within acceptable limits,ranging from 6.6%to 16.8%for native breeds and 6.1%to 18.1%for imported and bred breeds.The imported and bred pigs outperformed the native breeds in terms of sperm motility(50.42%to 69.58%vs.41.63%to 48.37%),farrowing rate(71.13%to 86.70%vs.57.15%to 74.35%),and litter size(7.74 to 10.30 vs.6.39 to 7.45).[Conclusions]These findings suggest that long-term selective breeding and domestication of native Guizhou pigs are necessary to enhance their reproductive performance.
