The accumulation of^(222)Rn and^(220)Rn progeny in poorly ventilated environments poses the risk of natural radiation exposure to the public.A previous study indicated that satisfactory results in determining the^(222...The accumulation of^(222)Rn and^(220)Rn progeny in poorly ventilated environments poses the risk of natural radiation exposure to the public.A previous study indicated that satisfactory results in determining the^(222)Rn and^(220)Rn progeny concentrations by measuring the total alpha counts at five time intervals within 560 min should be expected only in the case of high progeny concentrations in air.To complete the measurement within a relatively short period and adapt it for simultaneous measurements at comparatively lower^(222)Rn and^(220)Rn progeny concentrations,a novel mathematical model was proposed based on the radioactive decay law.This model employs a nonlinear fitting method to distinguish nuclides with overlapping spectra by utilizing the alpha particle counts of non-overlapping spectra within consecutive measurement cycles to obtain the concentrations of^(222)Rn and^(220)Rn progeny in air.Several verification experiments were conducted using an alpha spectrometer.The experimental results demonstrate that the concentrations of^(222)Rn and^(220)Rn progeny calculated by the new method align more closely with the actual circumstances than those calculated by the total count method,and their relative uncertainties are all within±16%.Furthermore,the measurement time was reduced to 90 min,representing an acceleration of 84%.The improved capability of the new method in distinguishing alpha particles with similar energies emitted from ^(218)Po and^(212)Bi,both approximately 6 MeV,contributed to realizing more accurate results.The proposed method has the potential advantage of measuring relatively low concentrations of^(222)Rn and^(220)Rn progeny in air more quickly via air filtration.展开更多
We used spatial, global trend and post-blocking analysis to examine the effectiveness of a progeny trial in a tree breeding program for Chinese fir (Cunninghamia lanceolata (Lamb.) Hook) on a hilly site with an en...We used spatial, global trend and post-blocking analysis to examine the effectiveness of a progeny trial in a tree breeding program for Chinese fir (Cunninghamia lanceolata (Lamb.) Hook) on a hilly site with an environmental gradient from hill top to bottom. Diameter at breast height (DBH) and tree height data had significant spatial auto-correlations among rows and columns. Adding a firstorder separable autoregressive term more effectively modelled the spatial variation than did the incomplete block (IB) model used for the experimental design. The spatial model also accounted for effects of experimental design factors and greatly reduced residual variances. The spatial analysis rel- ative to the IB analysis improved estimation of genetic parameters with the residual variance reduced 13 and 19% for DBH and tree height, respectively; heritability increased 35 and 51% for DBH and tree height, respectively; and genetic gain improved 3-5%. Fitting global trend and postblocking did not improve the analyses under IB model. The use of a spatial model or combined with a design model is recommended for forest genetic trials, particularly with global trend and local spatial variation of hilly sites.展开更多
Inhalation of 222Rn progeny in the domestic environment contributes the greatest fraction of the natural radiation exposure to the public. The ultrafine activity of these progeny amounts up to about l 0 percent of the...Inhalation of 222Rn progeny in the domestic environment contributes the greatest fraction of the natural radiation exposure to the public. The ultrafine activity of these progeny amounts up to about l 0 percent of the total activity (attached and ultrafine), but is considered to yield about 50 percent of the total radiation dose. Therefore, measurements of ultrafine fraction are essential for the estimation of radiation dose. The current study presents measured data on the total equilibrium equivalent concentration (EEC) and ultrafine equilibrium equivalent concentration (EECUn), ultrafine fraction (fb), attached and unattached activity size distributions of radon progeny in the low ventilated rooms at Minia University, Minia city, Egypt. A screen diffusion battery was used for collection the ultrafine fraction and measuring the total activity concentration of radon progeny. The attached activity size distribution of 214pb is determined by using a low pressure cascade impactor. The EEC of radon progeny varied between 1.3 and 18.9 Bq/m3 with a mean value of 5.2 ± 0.48 Bq/m2. The mean activity thermodynamic diameter (AMTD) ofultrafine of radon progeny was determined to be 1.26 nm with relative mean geometric standard deviations (GSD) of 1.3. The ultrafine fraction of radon progeny, fb, has a range 0.01 to 0.21 with an average of 0.08 ± 0.03. A relative mean GSD of 2.7 was determined for attached 2Lapb at a mean active median aerodynamic diameter (AMD) of 350 nm. Based on the above experimental results, the deposition fractions have been evaluated in each air way generation through the human lung by applying a lung deposition model. The bronchial deposition efficiencies of particles in the size range of attached radon progeny were found to be lower than those of ultrafine progeny. The effect of radon progeny deposition by adult male has been also studied for various levels of physical exertion. The dose conversion factor has been discussed as a function of fb.展开更多
Broiler breeder vaccination against IBD is usually based on the injection of at least one inactivated vaccine in oil adjuvant, typically included in a combined vaccine. Priming using one or several IBD vaccine (s) has...Broiler breeder vaccination against IBD is usually based on the injection of at least one inactivated vaccine in oil adjuvant, typically included in a combined vaccine. Priming using one or several IBD vaccine (s) has been the most common way to immunize the breeders so far. In summary, protection against vvIBD challenge in chicks of one commercial genetic line vaccinated in ovo with the HVT-IBD vector vaccine was demonstrated. The parents’ IBD vaccination program, using the HVT-IBD vector vaccine alone, the HVT-IBD vector vaccine plus IBD inactivated vaccine, and inactivated IBD vaccine alone, did not impair their progeny’s in ovo HVT-IBD vector vaccine take and subsequent protection against vvIBD virus challenge. An advantage in terms of immunization of the progeny against vvIBD was shown in the chicks born to breeders vaccinated with the HVT-IBD vaccine as a primer, as compared to breeders vaccinated with the inactivated vaccine alone. High level of IBD maternally-derived antibodies transmitted to the progeny by their parents induces together with an early onset of immunity by in ovo injection of a HVT-IBD vector vaccine clinical protection, as monitored on bursas, after vvIBD virus challenge.展开更多
Fraxinus mandshurica is one of the ancient relict flora species originated from the Tertiary period, which is mainly produced in the eastern mountains in Northeast China. It has an important scientific value for study...Fraxinus mandshurica is one of the ancient relict flora species originated from the Tertiary period, which is mainly produced in the eastern mountains in Northeast China. It has an important scientific value for studying the Tertiary flora and the Quaternary glacial climate. In order to make rational use of the natural genetic resources of F. mandshurica , and to increase the scientific and technological added value of improved varieties, this study used the method of positional observation of fixed standard fields to discuss the genetic variations of tree height and DBH of the natural populations and its open-pollinated progenies, systematically analyze the heritability and genetic gains in growth characters based on the analysis of progeny test, growth dynamic and heredity effects. The populations with good growth, stress resistance and adaptability were selected. The results showed that there were extremely significant difference in the growth characters among the natural populations, and the coefficients of variance for tree height (22.99%) were smaller, while the coefficients of variance for DBH (41.37%) were larger. On the other hand, there were extremely significant differences in the growth characters among the open-pollinated progenies, and the interaction between these progenies and the blocks was significant. Meanwhile, the growth characters had strong heritability, high genetic gain, and remarkable heredity effects. The heritability of tree height and DBH were 0.825 and 0.781, and the genetic gains were 15.16% and 25.85%, respectively. Therefore, Shanhetun (SHT) and Xinglong (XIL) were determined to be excellent natural populations both in the growth, resistance and adaptation with growth traits as the main evaluation index, which could be widely applied in the advanced generation improvement breeding.展开更多
In the present study, the effect of one-generation divergent selection on the growth and survival of the bay scallop (Argopecten irradians concentricus) was examined to evaluate the efficacy of a selection program c...In the present study, the effect of one-generation divergent selection on the growth and survival of the bay scallop (Argopecten irradians concentricus) was examined to evaluate the efficacy of a selection program currently being carried out in Beibu Bay in the South China Sea. A total of 146 adult scallops were randomly selected from the same cultured population ofA. i. concentricus, and divided into two groups in shell length (anterior-posterior measurement): large (4.91-6.02 cm, n=74) and small (3.31-4.18 cm, n=72). At the same time, a control group was also randomly sampled (4.21-4.88 cm, n=80). Mass-spawned F1 progenies from the three size groups were obtained and reared under identical conditions at all growth phases. The effects of two-way (or upward-downward) selection on fertilization rate, hatching rate, survival rate, daily growth in shell length and body weight were assessed in the three size groups. Results show that significant differences (P〈0.01) were found in hatching rate, survival rate and daily growth of F~ progenies, but not in fertilization rate (P〉0.05), among the three groups. The hatching rate, survival rate and daily growth of the progeny of large-sized parents were greater than those of the control group (P〈0.05), which in turn were larger than those of small-sized group (P〈0.05). Responses to selection by shell length and body weight were 0.32±0.04 cm and 2.18±0.05 g, respectively, for the upward selection, and -0.14±0.03 cm and -2.77±0.06 g, respectively, for the downward selection. The realized heritability estimates of shell length and body weight were 0.38±0.06 cm and 0.22±0.07 g for the upward selection, and 0.24±0.06 cm and 0.37±0.09 g for the downward selection, respectively. The change in growth by bidirectional selection suggests that high genetic variation may be present in the cultured bay scallop population in China.展开更多
The effects of genetic and environmental factors on Pinus koraiensis growth were studied based on a35 year-old progeny trial composed of open-pollinated offspring of twenty-one plus trees.Height,DBH and volume of the ...The effects of genetic and environmental factors on Pinus koraiensis growth were studied based on a35 year-old progeny trial composed of open-pollinated offspring of twenty-one plus trees.Height,DBH and volume of the offspring was analyzed using restricted maximum likelihood/best linear unbiased prediction in mixed model analysis.Significant site and family effects on the three traits were observed.The distinct growth of offspring by site with disparate climates corroborated the importance of planting species in suitable conditions.Growth differences by family was significant,emphasizing the importance of identifying families with either superior or inferior performance.The parental ranking was assigned in the sites,inferring the breeding value of each plus tree.The estimates of individual heritability(h_(i)^(2))of height,DBH and volume growth were 0.169-0.645,0.108-0.331 and 0.129-0.343 respectively,with higher h_(i)^(2) of the height than DBH on each site.Coefficient of variance of genetic effect was higher with DBH in some cases,indicating the scope for selection is larger for this trait despite the lower heritability compared to height.For the variation between families in terms of the performance stability across sites,consideration of the genotype by environment interaction is required in selecting materials to be used in reforestation with Korean pine.A few families with either superior or inferior performance retained their parental ranking for at least a decade.Other families with increased growth on a particular site were identified,indicating their high breeding value and low stability.Differences in the genetic performance of the families by site requires delineation of the breeding region of the species.展开更多
Atmospheric concentrations of radon(^(222)Rn) gas and its short-lived progenies^(218)Po,^(214)Pb, and^(214)Po were continuously monitored every four hours at the ground level in Jeddah city, Kingdom of Saudi...Atmospheric concentrations of radon(^(222)Rn) gas and its short-lived progenies^(218)Po,^(214)Pb, and^(214)Po were continuously monitored every four hours at the ground level in Jeddah city, Kingdom of Saudi Arabia. The measurements were performed three times every week, starting from November 2014 to October 2015. A method of electrostatic precipitation of positively charged^(218)Po and ^(214)Po by a positive voltage was applied for determining^(222)Rn gas concentration. The short-lived^(222)Rn progeny concentration was determined by using a filter holder connected with the alpha-spectrometric technique. The meteorological parameters(relative air humidity, air temperature, and wind speed) were determined during the measurements of^(222)Rn and its progeny concentrations.^(222)Rn gas as well as its short-lived progeny concentration display a daily and seasonal variation with high values in the night and early morning hours as compared to low values at noon and in the afternoon. The observed monthly atmospheric concentrations showed a seasonal trend with the highest values in the autumn/winter season and the lowest values in the spring/summer season. Moreover, and in parallel with alphaspectrometric measurements, a single filter-holder was used to collect air samples. The deposited activities of^(214)Pb and the long-lived^(222)Rn daughter ^(210)Pb on the filter were measured with the gamma spectrometric technique. The measured activity concentrations of^(214)Pb by both techniques were found to be relatively equal largely. The highest mean seasonally activity concentrations of ^(210)Pb were observed in the autumn/winter season while the lowest mean were observed in the spring/summer season. The mean residence time(MRT) of aerosol particles in the atmospheric air could be estimated from the activity ratios of ^(210)Pb/^(214)Pb.展开更多
Radon concentrations in high background radiation areas in the south are higher than those of others in China, especially 220 Rn concentration is significantly high. Therefore, measurements of 222 Rn and...Radon concentrations in high background radiation areas in the south are higher than those of others in China, especially 220 Rn concentration is significantly high. Therefore, measurements of 222 Rn and 220 Rn concentrations should be carried out there. This paper introduces a large size collector of radon progeny and its applications. The collector is a sheet of polyvinyl chloride fiber with electrostatics of (-500 V)-(-700 V). Its size (60 mm in diameter) is larger than those of others (26 mm in diameter) that work with the same principle. The collector is more effective to adsorb radon progeny than most of others. The equipment of ZnS(Ag) Scintillation Counting System is available for large size collectors to detect radon progeny. Therefore, its sensitivity of measurement is higher than that of others. According to the different half lives of radon progeny, and based on both theory and experiments, a formula for discrimination and calculation of 222 Rn and 220 Rn concentrations is deduced. The 222 Rn and 220 Rn concentrations were surveyed with electrostatic collectors of radon progeny on the campus of commercial school and some other areas in Hainan, southern China. Neither 222 Rn nor 220 Rn concentration was found significantly high. However, several faults underground were delineated. The collector is also used to study radon transportation. Results indicate that radon changes regularly with date when it has transported for a certain distance. Velocities of radon migration in the four media are quite different. Radon migrates more quickly in vertical tube than in the horizontal tube.展开更多
[Objectives]This study was conducted to rationally and effectively use natural genetic resources of white birch(Betula platyphylla Suk.),and improve the scientific and technological content of improved varieties.[Meth...[Objectives]This study was conducted to rationally and effectively use natural genetic resources of white birch(Betula platyphylla Suk.),and improve the scientific and technological content of improved varieties.[Methods]With the progeny of the white birch populations naturally distributed in northern China as objects,based on genetic effect analysis and comprehensive evaluation,the genetic variation,heritability and genetic gain of the growth characters in the progeny forest were systematically analyzed,by which populations or individuals with good growth vigor and strong stress resistance and adaptability were finally selected.[Results]There were extremely significant differences in the growth characters between the natural populations of white birch in northern China.The variation was the smallest in tree height,followed by crown width,and largest in DBH,and the average coefficients of variation were 20.56%,32.25%and 33.00%,respectively.The differences in characters between progeny of the same population were also extremely significant.The growth characters of white birch were more controlled by genes,and the genetic gains were larger.The heritability of tree height,DBH and crown width was 0.971,0.816 and 0.576,respectively,and the genetic gains were 15.97%,21.54%and 13.87%,respectively.[Conclusions]With the growth characters as the main evaluation indexes,taking various influencing factors into account,the Shanxi[SX]population,Hebei[HB]population and Liaoning[LN]population were selected to be excellent populations,and LN305,SX516 and HB403 were excellent individuals,which are advised to be widely used in multi-generation genetic improvement breeding.展开更多
Based on the 6-year-old young forests of 40 cypress(Cupressus funebris) families from four provenances(Fengdu County, Youyang County and Zhong County of Chongqing City, and Qiandao Lake in Zhejiang Province), differen...Based on the 6-year-old young forests of 40 cypress(Cupressus funebris) families from four provenances(Fengdu County, Youyang County and Zhong County of Chongqing City, and Qiandao Lake in Zhejiang Province), differences in growth traits between families and provenances were studied to select excellent families, so as to provide necessary data support for the upgrading of cypress seed gardens and inferior thinning of seed gardens. The results showed that there were significant differences in tree height, diameter at one meter height, crown width and growth potential between families and provenances. The coefficients of variation among families were 10.25%, 7.19%, 5.91% and 0.98%, respectively, and the coefficients of variation in tree height and diameter at one meter height among provenances were 7.25% and 12.58%, respectively, indicating that the tree height and diameter at one meter height of the superior cypress tree families were rich in variation among families and provenances, and there was potential for high-generation breeding. According to the selection rate of 15%, six excellent families for afforestation(Feng 8, Shi 4, Feng 1, Ye 14, Shi 3, and Ye 6) were screened. The average diameter at one meter height, tree height and crown width of the six excellent families were, respectively, 2.97 cm, 3.39 and 0.78 m, which were 18.82%, 12.70% and 15.42% higher than the family average, respectively, and 15.09%, 9.79% and 15.53% higher than the control group, respectively.展开更多
Generation of genetic diversity is necessary in improving on the potential of cassava when faced with various biotic and abiotic challenges. Presently, cassava breeders are breeding for a number of traits, such as dro...Generation of genetic diversity is necessary in improving on the potential of cassava when faced with various biotic and abiotic challenges. Presently, cassava breeders are breeding for a number of traits, such as drought tolerance, early root bulking, yield, starch, beta-carotene, protein, dry matter, pest and disease resistance, by relying on genetic diversity that exists in manihot esculenta germplasm. Controlled pollination is one of the main methods used to generate genetic diversity in cassava. However, the process of controlled pollination especially in an open field is prone to contamination by illegitimate pollen right from the time of pollination, seed collection, nursery bed establishment to planting of the trials. Therefore, authentication of the progeny obtained from cassava crosses is very important for genetic studies. Twelve informative microsatellite markers were used to verify the authenticity of 364 F1 progeny thought to come from four controlled parental crosses. The transmission of each allele at nine microsatellite loci was tracked from parents to progeny in each of the four Namikonga-derived F1 cassava families. Out of the 364 F1 progeny, 317 (87.1%) were true-to-type, 44 (12.1%) were a product of self-pollination and 3 (0.8%) were a product of open pollination. The consistency of the results obtained using microsatellite markers makes this technique a reliable tool for assessing the purity of progeny generated from cassava crosses.展开更多
Flowering time is critically important for crop yield, and detection of its genetic factors with strongly associated DNA markers is necessary in breeding programs. This study was undertaken to validate the quantitativ...Flowering time is critically important for crop yield, and detection of its genetic factors with strongly associated DNA markers is necessary in breeding programs. This study was undertaken to validate the quantitative trait loci (QTLs) underlying flowering time of sorghum based on the association between genotypes at SSR marker loci and flowering time in F3 family lines from self-pollinated heterozygous F2 plants developed by crossing between "SC112"---an early flowering variety from Ethiopia and "Kikuchi Zairai"--a late flowering variety from Japan. The results showed that the SSR markers linked to the QTLs on sorghum chromosomes 1, 2, 3, 5b, 7 and 8b were significantly (P 〈 0.05) associated with flowering time, and these markers and the QTLs reported previously are valid. On the other hand, the genotypes at the marker locus SB596 of qFT1-2 on chromosome 1 was not significantly associated with flowering time. The valid DNA markers, SB258 in qFTI-1, SB 1512 in qFT2, SB 1839 in qFT3, SB3369 in qFT5b, SB4096 in qFT7 and SB4540 and SB4660 in qFT8b, might be useful for DNA-marker assisted breeding.展开更多
A thorough understanding of the quantitative trait loci (QTLs) that underlie agronomically important traits in crops would greatly increase agricultural productivity. Although advances have been made in QTL cloning,...A thorough understanding of the quantitative trait loci (QTLs) that underlie agronomically important traits in crops would greatly increase agricultural productivity. Although advances have been made in QTL cloning, the majority of QTLs remain unknown because of their low heritability and minor contributions to phenotypic performance. Here we summarize the key advantages and disad- vantages of current QTL fine-mapping methodologies, and then introduce a sequential QTL fine-mapping strategy based on both genotypes and phenotypes of progeny derived from recombinants. With this mapping strategy, experimental errors could be dramat- ically diminished so as to reveal the authentic genetic effect of target QTLs. The number of progeny required to detect QTLs atvarious R2 values was calculated, and the backcross generation suitable to start QTL fine-mapping was also estimated. This mapping strategy has proved to be very powerful in narrowing down QTL regions, particularly minor-effect QTLs, as revealed by fine-mapping of various resistance QTLs in maize. Application of this sequential QTL mapping strategy should accelerate cloning of agronomically important QTLs, which is currently a substantial challenge in crops.展开更多
In this paper,we form a method to calculate the probability generating function of the total progeny of multitype branching process.As examples,we calculate probability generating function of the total progeny of the ...In this paper,we form a method to calculate the probability generating function of the total progeny of multitype branching process.As examples,we calculate probability generating function of the total progeny of the multitype branching processes within random walk which could stay at its position and(2-1) random walk.Consequently,we could give the probability generating functions and the distributions of the first passage time of corresponding random walks.Especially,for recurrent random walk which could stay at its position with probability 0 〈 r 〈 1,we show that the tail probability of the first passage time decays as 2/√π(1-r)1/√n= when n →∞.展开更多
The origin and the recent progress of pollen_tube pathway method (PTPM) is described. The results of transforming wheat during 1990 to 1994 are reported briefly. They are: (ⅰ) Transformation frequencies were 3%-6%, e...The origin and the recent progress of pollen_tube pathway method (PTPM) is described. The results of transforming wheat during 1990 to 1994 are reported briefly. They are: (ⅰ) Transformation frequencies were 3%-6%, even reached 13.5%; (ⅱ) the genetic expression of four continuous generations obtained from single individuals of transgenic plants by self_fertilization was investigated. Plants with higher expression of foreign gene were found in every generation (from T1 to T4), but a phenomenon of gene silence, in which the genotype contained foreign gene but did not express, was also observed. In addition, from the angle of fertilization biology the transformation mechanism of PTPM (or PTDM) was analyzed and discussed. It was considered that the essential principle of this method was just like that of protoplast method.展开更多
The impact of the radiation dose produced by^(222)Rn/^(220)Rn and its progeny on human health has garnered increasing interest in the nuclear research field.The establishment of robust,regulatory,and competent^(220)Rn...The impact of the radiation dose produced by^(222)Rn/^(220)Rn and its progeny on human health has garnered increasing interest in the nuclear research field.The establishment of robust,regulatory,and competent^(220)Rn chambers is crucial for accurately measuring radioactivity levels.However,studying the uniformity of the^(220)Rn progeny through experimental methods is challenging,because measuring the concentration of^(220)Rn and its progeny in multiple spatial locations simultaneously and in real time using experimental methods is difficult.Therefore,achieving precise control of the concentration of^(220)Rn and its progeny as well as the reliable sampling of the progeny pose significant challenges.To solve this problem,this study uses computational fluid dynamics to obtain the flow-field data of the^(220)Rn chamber under different wind speeds and progenyreplenishment rates.Qualitative analysis of the concentration distribution of the progeny and quantitative analysis of the progeny concentration and uniformity of the progeny concentration are conducted.The research findings indicated that the progeny concentration level is primarily influenced by wind speed and the progeny-complement rate.Wind speed also plays a crucial role in determining progeny concentration uniformity,whereas the progeny-complement rate has minimal impact on uniformity.To ensure the accuracy of^(220)Rn progeny concentration sampling,we propose a methodology for selecting an appropriate sampling area based on varying progeny concentrations.This study holds immense importance for enhancing the regulation and measurement standards of^(220)Rn and its progeny.展开更多
The oil palm (Elaeisguineensis Jacq) is used worldwide in commercial agriculture for the production of palm oil, palm kernel oil and palm wine. It produces more oil per plant than any other oil-producing crop in the w...The oil palm (Elaeisguineensis Jacq) is used worldwide in commercial agriculture for the production of palm oil, palm kernel oil and palm wine. It produces more oil per plant than any other oil-producing crop in the world. Production is constrained by several factors among which pests/diseases are of utmost importance. Vascular wilt (VW) caused by Fusarium oxysporum is the most devastating disease infecting this crop. Its soil-borne ecology has made the use of fungicides to manage this disease too expensive and inpragmatic. There is need for concerted research in the breeding and selection of wilt-tolerant progenies as an essential step in the management of Fusarium wilt disease. The study aims to assess the incidence and severity of vascular wilt among tested oil palm progenies, to evaluate the reduction in yield caused by the disease in the susceptible progenies and to identify the wilt-tolerant, high-yielding progenies. The study was carried out at Pamol Plantations Limited (PPL) in Ndian Estate (Ndian Division), in the Southwest Region of Cameroon. Three field trials were evaluated for tolerance/susceptibility to Fusarium wilt. Each trial consisted of 15 oil palm progenies replicated 4 times. Each progeny had 25 oil palm stands in each replicate. Hence, a total of 1500 oil palm stands were assessed. The experimental design was a randomized complete block (RCB) with trial codes: Trial 2001/1, planted in 2001;Trial 2001/2, planted in 2002;Trial 2001/3, planted in 2003. Each trail had an area of 12 ha, with a plant density of 143 palms·ha−1. Wilt incidence, severity, index, and yield were evaluated on 45 progenies from the 3 trails after identifying Fusarium oxysporum from oil palm plant part. Data was subjected to analysis of variance, Fischer’s least significant difference test (LSD) for mean separation. Identification of Fusarium was based on descriptive analysis. Incidence of VW in the 3 trials ranged from 1% - 39%. Also, 45% of infected plants were from progeny 676 while 1% was from progenies 689, 693, 694 and 710. Disease severity was from 0.9 in progeny 686 to 4.55 in 676. Wilt index ranged from 131 for progeny 694 and 710 to 495 for progenies 705. Out of the 45 progenies evaluated, 27 were tolerant (1 < 100) and 18 susceptible (1 ≥ 100). Within the tolerant progenies, 4 were significant (1 < 20) while 5 out of 18 were significantly susceptible (1 ≥ 185). Mean yield reduction of the susceptible progenies was 34.8% while in the tolerant progenies, it increased by 9.5% when compared to their controls. Progenies 702, 703 and 709 are recommended for planting based on the level of tolerance to Fusarium wilt disease and yield.展开更多
This study addresses the increasing demand for large-diameter production timber,and considers the time and space variability of half-sib families of Pinus massoniana.Height,diameter at breast height(DBH)and timber vol...This study addresses the increasing demand for large-diameter production timber,and considers the time and space variability of half-sib families of Pinus massoniana.Height,diameter at breast height(DBH)and timber volume of 440 open-pollinated half-sib progeny families were investigated in 14 progeny trials in different years and production regions.An evaluation of the genetic variation of all half-sib families was carried out during the sustainable rapid growth period and individual volumes were characterized as a major index.ANOVA analysis showed that there was considerable variance in the growth traits of most families in different years and on different sites.The variations caused by temporal and spatial changes of the mating system required three selection methods for analysis.The results show that there were differences among the heritabilities of different growth traits by different halfsib progenies.Average heritability values of height,DBH and volume were 0.33,0.34 and 0.36,respectively.Fortyfive superior families were selected in every progeny test,12 were selected in progeny trials by different years and five in different habitat progeny trials.Three superior families(Gui GC553A,Gui GC414A and Gui GC431A)were selected,although in different years and production regions.The genetic gains of timber volume of these selected r families ranged from 1.20 to 47.00%,which could provide a foundation for superior wood property selection and serve as material for seed improvement and extension in surrounding areas.展开更多
基金supported by the National Natural Science Foundation of China(No.12075112)Natural Science Foundation of Hunan(No.2023JJ50121),Natural Science Foundation of Hunan Province(No.2023JJ50091)Key Projects of Hunan Provincial Department of Education(No.23A0516).
文摘The accumulation of^(222)Rn and^(220)Rn progeny in poorly ventilated environments poses the risk of natural radiation exposure to the public.A previous study indicated that satisfactory results in determining the^(222)Rn and^(220)Rn progeny concentrations by measuring the total alpha counts at five time intervals within 560 min should be expected only in the case of high progeny concentrations in air.To complete the measurement within a relatively short period and adapt it for simultaneous measurements at comparatively lower^(222)Rn and^(220)Rn progeny concentrations,a novel mathematical model was proposed based on the radioactive decay law.This model employs a nonlinear fitting method to distinguish nuclides with overlapping spectra by utilizing the alpha particle counts of non-overlapping spectra within consecutive measurement cycles to obtain the concentrations of^(222)Rn and^(220)Rn progeny in air.Several verification experiments were conducted using an alpha spectrometer.The experimental results demonstrate that the concentrations of^(222)Rn and^(220)Rn progeny calculated by the new method align more closely with the actual circumstances than those calculated by the total count method,and their relative uncertainties are all within±16%.Furthermore,the measurement time was reduced to 90 min,representing an acceleration of 84%.The improved capability of the new method in distinguishing alpha particles with similar energies emitted from ^(218)Po and^(212)Bi,both approximately 6 MeV,contributed to realizing more accurate results.The proposed method has the potential advantage of measuring relatively low concentrations of^(222)Rn and^(220)Rn progeny in air more quickly via air filtration.
基金funded by the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(Grant No.15KJA220002)Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)Fujian Province Science and Technology Research funding on the fourth Tree Breeding Cycle Program of Chinese fir(Grant No.Min Lin 2016-1)
文摘We used spatial, global trend and post-blocking analysis to examine the effectiveness of a progeny trial in a tree breeding program for Chinese fir (Cunninghamia lanceolata (Lamb.) Hook) on a hilly site with an environmental gradient from hill top to bottom. Diameter at breast height (DBH) and tree height data had significant spatial auto-correlations among rows and columns. Adding a firstorder separable autoregressive term more effectively modelled the spatial variation than did the incomplete block (IB) model used for the experimental design. The spatial model also accounted for effects of experimental design factors and greatly reduced residual variances. The spatial analysis rel- ative to the IB analysis improved estimation of genetic parameters with the residual variance reduced 13 and 19% for DBH and tree height, respectively; heritability increased 35 and 51% for DBH and tree height, respectively; and genetic gain improved 3-5%. Fitting global trend and postblocking did not improve the analyses under IB model. The use of a spatial model or combined with a design model is recommended for forest genetic trials, particularly with global trend and local spatial variation of hilly sites.
文摘Inhalation of 222Rn progeny in the domestic environment contributes the greatest fraction of the natural radiation exposure to the public. The ultrafine activity of these progeny amounts up to about l 0 percent of the total activity (attached and ultrafine), but is considered to yield about 50 percent of the total radiation dose. Therefore, measurements of ultrafine fraction are essential for the estimation of radiation dose. The current study presents measured data on the total equilibrium equivalent concentration (EEC) and ultrafine equilibrium equivalent concentration (EECUn), ultrafine fraction (fb), attached and unattached activity size distributions of radon progeny in the low ventilated rooms at Minia University, Minia city, Egypt. A screen diffusion battery was used for collection the ultrafine fraction and measuring the total activity concentration of radon progeny. The attached activity size distribution of 214pb is determined by using a low pressure cascade impactor. The EEC of radon progeny varied between 1.3 and 18.9 Bq/m3 with a mean value of 5.2 ± 0.48 Bq/m2. The mean activity thermodynamic diameter (AMTD) ofultrafine of radon progeny was determined to be 1.26 nm with relative mean geometric standard deviations (GSD) of 1.3. The ultrafine fraction of radon progeny, fb, has a range 0.01 to 0.21 with an average of 0.08 ± 0.03. A relative mean GSD of 2.7 was determined for attached 2Lapb at a mean active median aerodynamic diameter (AMD) of 350 nm. Based on the above experimental results, the deposition fractions have been evaluated in each air way generation through the human lung by applying a lung deposition model. The bronchial deposition efficiencies of particles in the size range of attached radon progeny were found to be lower than those of ultrafine progeny. The effect of radon progeny deposition by adult male has been also studied for various levels of physical exertion. The dose conversion factor has been discussed as a function of fb.
文摘Broiler breeder vaccination against IBD is usually based on the injection of at least one inactivated vaccine in oil adjuvant, typically included in a combined vaccine. Priming using one or several IBD vaccine (s) has been the most common way to immunize the breeders so far. In summary, protection against vvIBD challenge in chicks of one commercial genetic line vaccinated in ovo with the HVT-IBD vector vaccine was demonstrated. The parents’ IBD vaccination program, using the HVT-IBD vector vaccine alone, the HVT-IBD vector vaccine plus IBD inactivated vaccine, and inactivated IBD vaccine alone, did not impair their progeny’s in ovo HVT-IBD vector vaccine take and subsequent protection against vvIBD virus challenge. An advantage in terms of immunization of the progeny against vvIBD was shown in the chicks born to breeders vaccinated with the HVT-IBD vaccine as a primer, as compared to breeders vaccinated with the inactivated vaccine alone. High level of IBD maternally-derived antibodies transmitted to the progeny by their parents induces together with an early onset of immunity by in ovo injection of a HVT-IBD vector vaccine clinical protection, as monitored on bursas, after vvIBD virus challenge.
基金Supported by the Project of Heilongjiang Provincial Science and Technology Department(GC04B116)the Forest Germplasm Resources Platform Operation Service Project of China(2011DKA21003-02)the Special Fund Project for the Scientific Research of the Forest Public Welfare Industry of China(201204307-07)
文摘Fraxinus mandshurica is one of the ancient relict flora species originated from the Tertiary period, which is mainly produced in the eastern mountains in Northeast China. It has an important scientific value for studying the Tertiary flora and the Quaternary glacial climate. In order to make rational use of the natural genetic resources of F. mandshurica , and to increase the scientific and technological added value of improved varieties, this study used the method of positional observation of fixed standard fields to discuss the genetic variations of tree height and DBH of the natural populations and its open-pollinated progenies, systematically analyze the heritability and genetic gains in growth characters based on the analysis of progeny test, growth dynamic and heredity effects. The populations with good growth, stress resistance and adaptability were selected. The results showed that there were extremely significant difference in the growth characters among the natural populations, and the coefficients of variance for tree height (22.99%) were smaller, while the coefficients of variance for DBH (41.37%) were larger. On the other hand, there were extremely significant differences in the growth characters among the open-pollinated progenies, and the interaction between these progenies and the blocks was significant. Meanwhile, the growth characters had strong heritability, high genetic gain, and remarkable heredity effects. The heritability of tree height and DBH were 0.825 and 0.781, and the genetic gains were 15.16% and 25.85%, respectively. Therefore, Shanhetun (SHT) and Xinglong (XIL) were determined to be excellent natural populations both in the growth, resistance and adaptation with growth traits as the main evaluation index, which could be widely applied in the advanced generation improvement breeding.
基金Supported by the National Science and Technology Ministry(No.2007GB2E000183)the Agricultural Ministry of China(No.nyhyzx07-047)
文摘In the present study, the effect of one-generation divergent selection on the growth and survival of the bay scallop (Argopecten irradians concentricus) was examined to evaluate the efficacy of a selection program currently being carried out in Beibu Bay in the South China Sea. A total of 146 adult scallops were randomly selected from the same cultured population ofA. i. concentricus, and divided into two groups in shell length (anterior-posterior measurement): large (4.91-6.02 cm, n=74) and small (3.31-4.18 cm, n=72). At the same time, a control group was also randomly sampled (4.21-4.88 cm, n=80). Mass-spawned F1 progenies from the three size groups were obtained and reared under identical conditions at all growth phases. The effects of two-way (or upward-downward) selection on fertilization rate, hatching rate, survival rate, daily growth in shell length and body weight were assessed in the three size groups. Results show that significant differences (P〈0.01) were found in hatching rate, survival rate and daily growth of F~ progenies, but not in fertilization rate (P〉0.05), among the three groups. The hatching rate, survival rate and daily growth of the progeny of large-sized parents were greater than those of the control group (P〈0.05), which in turn were larger than those of small-sized group (P〈0.05). Responses to selection by shell length and body weight were 0.32±0.04 cm and 2.18±0.05 g, respectively, for the upward selection, and -0.14±0.03 cm and -2.77±0.06 g, respectively, for the downward selection. The realized heritability estimates of shell length and body weight were 0.38±0.06 cm and 0.22±0.07 g for the upward selection, and 0.24±0.06 cm and 0.37±0.09 g for the downward selection, respectively. The change in growth by bidirectional selection suggests that high genetic variation may be present in the cultured bay scallop population in China.
文摘The effects of genetic and environmental factors on Pinus koraiensis growth were studied based on a35 year-old progeny trial composed of open-pollinated offspring of twenty-one plus trees.Height,DBH and volume of the offspring was analyzed using restricted maximum likelihood/best linear unbiased prediction in mixed model analysis.Significant site and family effects on the three traits were observed.The distinct growth of offspring by site with disparate climates corroborated the importance of planting species in suitable conditions.Growth differences by family was significant,emphasizing the importance of identifying families with either superior or inferior performance.The parental ranking was assigned in the sites,inferring the breeding value of each plus tree.The estimates of individual heritability(h_(i)^(2))of height,DBH and volume growth were 0.169-0.645,0.108-0.331 and 0.129-0.343 respectively,with higher h_(i)^(2) of the height than DBH on each site.Coefficient of variance of genetic effect was higher with DBH in some cases,indicating the scope for selection is larger for this trait despite the lower heritability compared to height.For the variation between families in terms of the performance stability across sites,consideration of the genotype by environment interaction is required in selecting materials to be used in reforestation with Korean pine.A few families with either superior or inferior performance retained their parental ranking for at least a decade.Other families with increased growth on a particular site were identified,indicating their high breeding value and low stability.Differences in the genetic performance of the families by site requires delineation of the breeding region of the species.
基金Project supported by the Deanship of Scientific Research(DSR),King Abdulaziz UniversityJeddah(Grant No.291/965/1434)
文摘Atmospheric concentrations of radon(^(222)Rn) gas and its short-lived progenies^(218)Po,^(214)Pb, and^(214)Po were continuously monitored every four hours at the ground level in Jeddah city, Kingdom of Saudi Arabia. The measurements were performed three times every week, starting from November 2014 to October 2015. A method of electrostatic precipitation of positively charged^(218)Po and ^(214)Po by a positive voltage was applied for determining^(222)Rn gas concentration. The short-lived^(222)Rn progeny concentration was determined by using a filter holder connected with the alpha-spectrometric technique. The meteorological parameters(relative air humidity, air temperature, and wind speed) were determined during the measurements of^(222)Rn and its progeny concentrations.^(222)Rn gas as well as its short-lived progeny concentration display a daily and seasonal variation with high values in the night and early morning hours as compared to low values at noon and in the afternoon. The observed monthly atmospheric concentrations showed a seasonal trend with the highest values in the autumn/winter season and the lowest values in the spring/summer season. Moreover, and in parallel with alphaspectrometric measurements, a single filter-holder was used to collect air samples. The deposited activities of^(214)Pb and the long-lived^(222)Rn daughter ^(210)Pb on the filter were measured with the gamma spectrometric technique. The measured activity concentrations of^(214)Pb by both techniques were found to be relatively equal largely. The highest mean seasonally activity concentrations of ^(210)Pb were observed in the autumn/winter season while the lowest mean were observed in the spring/summer season. The mean residence time(MRT) of aerosol particles in the atmospheric air could be estimated from the activity ratios of ^(210)Pb/^(214)Pb.
文摘Radon concentrations in high background radiation areas in the south are higher than those of others in China, especially 220 Rn concentration is significantly high. Therefore, measurements of 222 Rn and 220 Rn concentrations should be carried out there. This paper introduces a large size collector of radon progeny and its applications. The collector is a sheet of polyvinyl chloride fiber with electrostatics of (-500 V)-(-700 V). Its size (60 mm in diameter) is larger than those of others (26 mm in diameter) that work with the same principle. The collector is more effective to adsorb radon progeny than most of others. The equipment of ZnS(Ag) Scintillation Counting System is available for large size collectors to detect radon progeny. Therefore, its sensitivity of measurement is higher than that of others. According to the different half lives of radon progeny, and based on both theory and experiments, a formula for discrimination and calculation of 222 Rn and 220 Rn concentrations is deduced. The 222 Rn and 220 Rn concentrations were surveyed with electrostatic collectors of radon progeny on the campus of commercial school and some other areas in Hainan, southern China. Neither 222 Rn nor 220 Rn concentration was found significantly high. However, several faults underground were delineated. The collector is also used to study radon transportation. Results indicate that radon changes regularly with date when it has transported for a certain distance. Velocities of radon migration in the four media are quite different. Radon migrates more quickly in vertical tube than in the horizontal tube.
基金Supported by Heilongjiang Provincial Science and Technology Department Project(GC04B116)National Forest Tree Germplasm Resource Platform Running Service Project(2011DKA21003-02)National Forestry Public Welfare Industry Research Project(201204307-07)
文摘[Objectives]This study was conducted to rationally and effectively use natural genetic resources of white birch(Betula platyphylla Suk.),and improve the scientific and technological content of improved varieties.[Methods]With the progeny of the white birch populations naturally distributed in northern China as objects,based on genetic effect analysis and comprehensive evaluation,the genetic variation,heritability and genetic gain of the growth characters in the progeny forest were systematically analyzed,by which populations or individuals with good growth vigor and strong stress resistance and adaptability were finally selected.[Results]There were extremely significant differences in the growth characters between the natural populations of white birch in northern China.The variation was the smallest in tree height,followed by crown width,and largest in DBH,and the average coefficients of variation were 20.56%,32.25%and 33.00%,respectively.The differences in characters between progeny of the same population were also extremely significant.The growth characters of white birch were more controlled by genes,and the genetic gains were larger.The heritability of tree height,DBH and crown width was 0.971,0.816 and 0.576,respectively,and the genetic gains were 15.97%,21.54%and 13.87%,respectively.[Conclusions]With the growth characters as the main evaluation indexes,taking various influencing factors into account,the Shanxi[SX]population,Hebei[HB]population and Liaoning[LN]population were selected to be excellent populations,and LN305,SX516 and HB403 were excellent individuals,which are advised to be widely used in multi-generation genetic improvement breeding.
基金Supported by Assistance Fund for National Seed Base of Forest Tree
文摘Based on the 6-year-old young forests of 40 cypress(Cupressus funebris) families from four provenances(Fengdu County, Youyang County and Zhong County of Chongqing City, and Qiandao Lake in Zhejiang Province), differences in growth traits between families and provenances were studied to select excellent families, so as to provide necessary data support for the upgrading of cypress seed gardens and inferior thinning of seed gardens. The results showed that there were significant differences in tree height, diameter at one meter height, crown width and growth potential between families and provenances. The coefficients of variation among families were 10.25%, 7.19%, 5.91% and 0.98%, respectively, and the coefficients of variation in tree height and diameter at one meter height among provenances were 7.25% and 12.58%, respectively, indicating that the tree height and diameter at one meter height of the superior cypress tree families were rich in variation among families and provenances, and there was potential for high-generation breeding. According to the selection rate of 15%, six excellent families for afforestation(Feng 8, Shi 4, Feng 1, Ye 14, Shi 3, and Ye 6) were screened. The average diameter at one meter height, tree height and crown width of the six excellent families were, respectively, 2.97 cm, 3.39 and 0.78 m, which were 18.82%, 12.70% and 15.42% higher than the family average, respectively, and 15.09%, 9.79% and 15.53% higher than the control group, respectively.
文摘Generation of genetic diversity is necessary in improving on the potential of cassava when faced with various biotic and abiotic challenges. Presently, cassava breeders are breeding for a number of traits, such as drought tolerance, early root bulking, yield, starch, beta-carotene, protein, dry matter, pest and disease resistance, by relying on genetic diversity that exists in manihot esculenta germplasm. Controlled pollination is one of the main methods used to generate genetic diversity in cassava. However, the process of controlled pollination especially in an open field is prone to contamination by illegitimate pollen right from the time of pollination, seed collection, nursery bed establishment to planting of the trials. Therefore, authentication of the progeny obtained from cassava crosses is very important for genetic studies. Twelve informative microsatellite markers were used to verify the authenticity of 364 F1 progeny thought to come from four controlled parental crosses. The transmission of each allele at nine microsatellite loci was tracked from parents to progeny in each of the four Namikonga-derived F1 cassava families. Out of the 364 F1 progeny, 317 (87.1%) were true-to-type, 44 (12.1%) were a product of self-pollination and 3 (0.8%) were a product of open pollination. The consistency of the results obtained using microsatellite markers makes this technique a reliable tool for assessing the purity of progeny generated from cassava crosses.
文摘Flowering time is critically important for crop yield, and detection of its genetic factors with strongly associated DNA markers is necessary in breeding programs. This study was undertaken to validate the quantitative trait loci (QTLs) underlying flowering time of sorghum based on the association between genotypes at SSR marker loci and flowering time in F3 family lines from self-pollinated heterozygous F2 plants developed by crossing between "SC112"---an early flowering variety from Ethiopia and "Kikuchi Zairai"--a late flowering variety from Japan. The results showed that the SSR markers linked to the QTLs on sorghum chromosomes 1, 2, 3, 5b, 7 and 8b were significantly (P 〈 0.05) associated with flowering time, and these markers and the QTLs reported previously are valid. On the other hand, the genotypes at the marker locus SB596 of qFT1-2 on chromosome 1 was not significantly associated with flowering time. The valid DNA markers, SB258 in qFTI-1, SB 1512 in qFT2, SB 1839 in qFT3, SB3369 in qFT5b, SB4096 in qFT7 and SB4540 and SB4660 in qFT8b, might be useful for DNA-marker assisted breeding.
基金supported by the National Basic Research Program of China (973) (2009CB118401)the Hi-tech Research and Development Program of China (863)
文摘A thorough understanding of the quantitative trait loci (QTLs) that underlie agronomically important traits in crops would greatly increase agricultural productivity. Although advances have been made in QTL cloning, the majority of QTLs remain unknown because of their low heritability and minor contributions to phenotypic performance. Here we summarize the key advantages and disad- vantages of current QTL fine-mapping methodologies, and then introduce a sequential QTL fine-mapping strategy based on both genotypes and phenotypes of progeny derived from recombinants. With this mapping strategy, experimental errors could be dramat- ically diminished so as to reveal the authentic genetic effect of target QTLs. The number of progeny required to detect QTLs atvarious R2 values was calculated, and the backcross generation suitable to start QTL fine-mapping was also estimated. This mapping strategy has proved to be very powerful in narrowing down QTL regions, particularly minor-effect QTLs, as revealed by fine-mapping of various resistance QTLs in maize. Application of this sequential QTL mapping strategy should accelerate cloning of agronomically important QTLs, which is currently a substantial challenge in crops.
基金Supported by National Nature Science Foundation of China(Grant No.11226199)
文摘In this paper,we form a method to calculate the probability generating function of the total progeny of multitype branching process.As examples,we calculate probability generating function of the total progeny of the multitype branching processes within random walk which could stay at its position and(2-1) random walk.Consequently,we could give the probability generating functions and the distributions of the first passage time of corresponding random walks.Especially,for recurrent random walk which could stay at its position with probability 0 〈 r 〈 1,we show that the tail probability of the first passage time decays as 2/√π(1-r)1/√n= when n →∞.
文摘The origin and the recent progress of pollen_tube pathway method (PTPM) is described. The results of transforming wheat during 1990 to 1994 are reported briefly. They are: (ⅰ) Transformation frequencies were 3%-6%, even reached 13.5%; (ⅱ) the genetic expression of four continuous generations obtained from single individuals of transgenic plants by self_fertilization was investigated. Plants with higher expression of foreign gene were found in every generation (from T1 to T4), but a phenomenon of gene silence, in which the genotype contained foreign gene but did not express, was also observed. In addition, from the angle of fertilization biology the transformation mechanism of PTPM (or PTDM) was analyzed and discussed. It was considered that the essential principle of this method was just like that of protoplast method.
基金supported by the National Natural Science Foundation of China(Nos.12375310,12175102,and 118750356)Youth Talent Foundation of Hunan Province of China(2022TJQ16)Graduate Research and Innovation Projects of Hunan Province(CX20230964)。
文摘The impact of the radiation dose produced by^(222)Rn/^(220)Rn and its progeny on human health has garnered increasing interest in the nuclear research field.The establishment of robust,regulatory,and competent^(220)Rn chambers is crucial for accurately measuring radioactivity levels.However,studying the uniformity of the^(220)Rn progeny through experimental methods is challenging,because measuring the concentration of^(220)Rn and its progeny in multiple spatial locations simultaneously and in real time using experimental methods is difficult.Therefore,achieving precise control of the concentration of^(220)Rn and its progeny as well as the reliable sampling of the progeny pose significant challenges.To solve this problem,this study uses computational fluid dynamics to obtain the flow-field data of the^(220)Rn chamber under different wind speeds and progenyreplenishment rates.Qualitative analysis of the concentration distribution of the progeny and quantitative analysis of the progeny concentration and uniformity of the progeny concentration are conducted.The research findings indicated that the progeny concentration level is primarily influenced by wind speed and the progeny-complement rate.Wind speed also plays a crucial role in determining progeny concentration uniformity,whereas the progeny-complement rate has minimal impact on uniformity.To ensure the accuracy of^(220)Rn progeny concentration sampling,we propose a methodology for selecting an appropriate sampling area based on varying progeny concentrations.This study holds immense importance for enhancing the regulation and measurement standards of^(220)Rn and its progeny.
文摘The oil palm (Elaeisguineensis Jacq) is used worldwide in commercial agriculture for the production of palm oil, palm kernel oil and palm wine. It produces more oil per plant than any other oil-producing crop in the world. Production is constrained by several factors among which pests/diseases are of utmost importance. Vascular wilt (VW) caused by Fusarium oxysporum is the most devastating disease infecting this crop. Its soil-borne ecology has made the use of fungicides to manage this disease too expensive and inpragmatic. There is need for concerted research in the breeding and selection of wilt-tolerant progenies as an essential step in the management of Fusarium wilt disease. The study aims to assess the incidence and severity of vascular wilt among tested oil palm progenies, to evaluate the reduction in yield caused by the disease in the susceptible progenies and to identify the wilt-tolerant, high-yielding progenies. The study was carried out at Pamol Plantations Limited (PPL) in Ndian Estate (Ndian Division), in the Southwest Region of Cameroon. Three field trials were evaluated for tolerance/susceptibility to Fusarium wilt. Each trial consisted of 15 oil palm progenies replicated 4 times. Each progeny had 25 oil palm stands in each replicate. Hence, a total of 1500 oil palm stands were assessed. The experimental design was a randomized complete block (RCB) with trial codes: Trial 2001/1, planted in 2001;Trial 2001/2, planted in 2002;Trial 2001/3, planted in 2003. Each trail had an area of 12 ha, with a plant density of 143 palms·ha−1. Wilt incidence, severity, index, and yield were evaluated on 45 progenies from the 3 trails after identifying Fusarium oxysporum from oil palm plant part. Data was subjected to analysis of variance, Fischer’s least significant difference test (LSD) for mean separation. Identification of Fusarium was based on descriptive analysis. Incidence of VW in the 3 trials ranged from 1% - 39%. Also, 45% of infected plants were from progeny 676 while 1% was from progenies 689, 693, 694 and 710. Disease severity was from 0.9 in progeny 686 to 4.55 in 676. Wilt index ranged from 131 for progeny 694 and 710 to 495 for progenies 705. Out of the 45 progenies evaluated, 27 were tolerant (1 < 100) and 18 susceptible (1 ≥ 100). Within the tolerant progenies, 4 were significant (1 < 20) while 5 out of 18 were significantly susceptible (1 ≥ 185). Mean yield reduction of the susceptible progenies was 34.8% while in the tolerant progenies, it increased by 9.5% when compared to their controls. Progenies 702, 703 and 709 are recommended for planting based on the level of tolerance to Fusarium wilt disease and yield.
文摘This study addresses the increasing demand for large-diameter production timber,and considers the time and space variability of half-sib families of Pinus massoniana.Height,diameter at breast height(DBH)and timber volume of 440 open-pollinated half-sib progeny families were investigated in 14 progeny trials in different years and production regions.An evaluation of the genetic variation of all half-sib families was carried out during the sustainable rapid growth period and individual volumes were characterized as a major index.ANOVA analysis showed that there was considerable variance in the growth traits of most families in different years and on different sites.The variations caused by temporal and spatial changes of the mating system required three selection methods for analysis.The results show that there were differences among the heritabilities of different growth traits by different halfsib progenies.Average heritability values of height,DBH and volume were 0.33,0.34 and 0.36,respectively.Fortyfive superior families were selected in every progeny test,12 were selected in progeny trials by different years and five in different habitat progeny trials.Three superior families(Gui GC553A,Gui GC414A and Gui GC431A)were selected,although in different years and production regions.The genetic gains of timber volume of these selected r families ranged from 1.20 to 47.00%,which could provide a foundation for superior wood property selection and serve as material for seed improvement and extension in surrounding areas.