Development of new technologies for evaluating genetically modiifed （GM） crops has revealed that there are unintended insertions and expression changes in GM crops. Proifling techniques are non-targeted approaches a...Development of new technologies for evaluating genetically modiifed （GM） crops has revealed that there are unintended insertions and expression changes in GM crops. Proifling techniques are non-targeted approaches and are capable of detecting more unintended changes in GM crops. Here, we report the application of a comparative proteomic approach to investigate the protein proifle differences between a GM rice line, which has a lysine-rich protein gene, and its non-transgenic parental line. Proteome analysis by two-dimensional gel electrophoresis （2-DE） and mass spectrum analysis of the seeds identiifed 22 differentially expressed protein spots. Apart from a number of glutelins that were detected as targeted proteins in the GM line, the majority of the other changed proteins were involved in carbohydrate metabolism, protein synthesis and stress responses. These results indicated that the altered proteins were not associated with plant allergens or toxicity.展开更多
We make use of the images from the Sloan Digital Sky Survey Stripe 82（Stripe 82） to present an analysis of r band surface brightness profiles and radial color gradients（g-r,u-r） in our sample of 111 nearby early-t...We make use of the images from the Sloan Digital Sky Survey Stripe 82（Stripe 82） to present an analysis of r band surface brightness profiles and radial color gradients（g-r,u-r） in our sample of 111 nearby early-type galaxies（ETGs） .Thanks to the Stripe 82 images,each of which is co-added from about 50 single frames,we are able to pay special attention to the low-surface-brightness areas（LSB areas） of the galaxies.The LSB areas affect the Sérsic fittings and concentration indices by making both of the indices less than the typical values for ETGs.In the Sérsic fits to all the surface brightness profiles,we found some Sérsic indices that range from 1.5 to 2.5,much smaller than those of typical de Vaucouleur profiles and relatively close to those of exponential disks,and some others much larger than four but still with accurate fitting.Two galaxies cannot be fitted with a single Sérsic profile,but once we try double Sérsic profiles,the fittings are improved：one with a profile relatively close to the de Vaucouleur law in the inner area and a profile relatively close to an exponential law in the LSB area,the other with a nice fitting in the inner area but still having a failed fitting in the outer area.About 60%of the sample has negative color gradients（red-core） within 1.5Re,much more than the approximately 10%positive ones（blue-core） within the same radius.However,taking into account the LSB areas,we find that the color gradients are not necessarily monotonic：about one third of the red-core（or blue-core） galaxies have positive（or negative） color gradients in the outer areas. So LSB areas not only make ETGs’Sérsic profiles deviate from de Vaucouleur ones and shift to the disk end,but also reveal that quite a number of ETGs have opposite color gradients in inner and outer areas.These outcomes remind us of the necessity of double-Sérsic fitting.These LSB phenomena may be interpreted by mergers and thus have different metallicity in the outer areas.Isophotal parameters are also discussed briefly in this paper with the following conclusion：there are more disky nearby ETGs that are identified than boxy ones.展开更多
Several planar waveguides have been fabricated. The waveguides have been polished for determination of their refractive index profiles (RIP) by wedge method. The RIP determined by inserting the sample in a Mach-Zehnde...Several planar waveguides have been fabricated. The waveguides have been polished for determination of their refractive index profiles (RIP) by wedge method. The RIP determined by inserting the sample in a Mach-Zehnder interferometer and applying fringe analysis methods.展开更多
Statistical inference on parametric part for the partially linear single-index model (PLSIM) is considered in this paper. A profile least-squares technique for estimating the parametric part is proposed and the asympt...Statistical inference on parametric part for the partially linear single-index model (PLSIM) is considered in this paper. A profile least-squares technique for estimating the parametric part is proposed and the asymptotic normality of the profile least-squares estimator is given. Based on the estimator, a generalized likelihood ratio (GLR) test is proposed to test whether parameters on linear part for the model is under a contain linear restricted condition. Under the null model, the proposed GLR statistic follows asymptotically the χ2-distribution with the scale constant and degree of freedom independent of the nuisance parameters, known as Wilks phenomenon. Both simulated and real data examples are used to illustrate our proposed methods.展开更多
From July to September of 1990,CH_4 flux measurements were made in Lin'an rice paddies using gradient profile techniques.Some characteristics of the turbulence structure under the stable conditions have been verif...From July to September of 1990,CH_4 flux measurements were made in Lin'an rice paddies using gradient profile techniques.Some characteristics of the turbulence structure under the stable conditions have been verified in the surface layer according to the in situ measurements.The semi-empirical turbulent parameters β_m,β_h and β_c and their changes with the stability parameter Ri are given.Observed results indicate that CH_4 flux in the rice-paddy is mainly decided by the methnogenesis and the process of CH4 transport from internal soils to the atmosphere,and that the CH_4 vertical transfer is depressed in the stable surface layer.The CH_4 flux shows that its obvious diurnal changes,and the mean CH_4 flux are higher in nighttime and lower in daytime,and the peaks appear at about 2000 BST at night and 0300 BST in the early morning,respectively.The mean value of CH_4 flux is about 4.18±2.3 mg/m^2 h.展开更多
基金supported by the National Major Special Project for the Development of Transgenic OrganismsChina(2009ZX-08011-003B)+2 种基金the Jiangsu Province Qing Lan Project for Young and Middle-Aged Academic LeadersChina and the Jiangsu Province Qing Lan Project for Outstanding Scientific and Technological Innovation TeamChina
文摘Development of new technologies for evaluating genetically modiifed （GM） crops has revealed that there are unintended insertions and expression changes in GM crops. Proifling techniques are non-targeted approaches and are capable of detecting more unintended changes in GM crops. Here, we report the application of a comparative proteomic approach to investigate the protein proifle differences between a GM rice line, which has a lysine-rich protein gene, and its non-transgenic parental line. Proteome analysis by two-dimensional gel electrophoresis （2-DE） and mass spectrum analysis of the seeds identiifed 22 differentially expressed protein spots. Apart from a number of glutelins that were detected as targeted proteins in the GM line, the majority of the other changed proteins were involved in carbohydrate metabolism, protein synthesis and stress responses. These results indicated that the altered proteins were not associated with plant allergens or toxicity.
基金Supported by the National Fund for Fostering Talents of Basic Sciences of China
文摘We make use of the images from the Sloan Digital Sky Survey Stripe 82（Stripe 82） to present an analysis of r band surface brightness profiles and radial color gradients（g-r,u-r） in our sample of 111 nearby early-type galaxies（ETGs） .Thanks to the Stripe 82 images,each of which is co-added from about 50 single frames,we are able to pay special attention to the low-surface-brightness areas（LSB areas） of the galaxies.The LSB areas affect the Sérsic fittings and concentration indices by making both of the indices less than the typical values for ETGs.In the Sérsic fits to all the surface brightness profiles,we found some Sérsic indices that range from 1.5 to 2.5,much smaller than those of typical de Vaucouleur profiles and relatively close to those of exponential disks,and some others much larger than four but still with accurate fitting.Two galaxies cannot be fitted with a single Sérsic profile,but once we try double Sérsic profiles,the fittings are improved：one with a profile relatively close to the de Vaucouleur law in the inner area and a profile relatively close to an exponential law in the LSB area,the other with a nice fitting in the inner area but still having a failed fitting in the outer area.About 60%of the sample has negative color gradients（red-core） within 1.5Re,much more than the approximately 10%positive ones（blue-core） within the same radius.However,taking into account the LSB areas,we find that the color gradients are not necessarily monotonic：about one third of the red-core（or blue-core） galaxies have positive（or negative） color gradients in the outer areas. So LSB areas not only make ETGs’Sérsic profiles deviate from de Vaucouleur ones and shift to the disk end,but also reveal that quite a number of ETGs have opposite color gradients in inner and outer areas.These outcomes remind us of the necessity of double-Sérsic fitting.These LSB phenomena may be interpreted by mergers and thus have different metallicity in the outer areas.Isophotal parameters are also discussed briefly in this paper with the following conclusion：there are more disky nearby ETGs that are identified than boxy ones.
文摘Several planar waveguides have been fabricated. The waveguides have been polished for determination of their refractive index profiles (RIP) by wedge method. The RIP determined by inserting the sample in a Mach-Zehnder interferometer and applying fringe analysis methods.
基金supported by National Natural Science Foundation of China (Grant No. 10871072)Natural Science Foundation of Shanxi Province of China (Grant No. 2007011014)PhD Program Scholarship Fund of ECNU 2009
文摘Statistical inference on parametric part for the partially linear single-index model (PLSIM) is considered in this paper. A profile least-squares technique for estimating the parametric part is proposed and the asymptotic normality of the profile least-squares estimator is given. Based on the estimator, a generalized likelihood ratio (GLR) test is proposed to test whether parameters on linear part for the model is under a contain linear restricted condition. Under the null model, the proposed GLR statistic follows asymptotically the χ2-distribution with the scale constant and degree of freedom independent of the nuisance parameters, known as Wilks phenomenon. Both simulated and real data examples are used to illustrate our proposed methods.
基金The project is sponsored by the National Natural Science Foundation of China.
文摘From July to September of 1990,CH_4 flux measurements were made in Lin'an rice paddies using gradient profile techniques.Some characteristics of the turbulence structure under the stable conditions have been verified in the surface layer according to the in situ measurements.The semi-empirical turbulent parameters β_m,β_h and β_c and their changes with the stability parameter Ri are given.Observed results indicate that CH_4 flux in the rice-paddy is mainly decided by the methnogenesis and the process of CH4 transport from internal soils to the atmosphere,and that the CH_4 vertical transfer is depressed in the stable surface layer.The CH_4 flux shows that its obvious diurnal changes,and the mean CH_4 flux are higher in nighttime and lower in daytime,and the peaks appear at about 2000 BST at night and 0300 BST in the early morning,respectively.The mean value of CH_4 flux is about 4.18±2.3 mg/m^2 h.
