Annulus fibrosus （AF） tissue engineering has recently received increasing attention as a treatment for intervertebral disc 0VD） degeneration; however, such engineering remains challenging because of the remarkable ...Annulus fibrosus （AF） tissue engineering has recently received increasing attention as a treatment for intervertebral disc 0VD） degeneration; however, such engineering remains challenging because of the remarkable complexity of AF tissue. In order to engineer a functional AF replacement, the fabrication of cell-scaffold constructs that mimic the cellular, biochemical and structural features of native AF tissue is critical. In this study, we fabricated aligned fibroua polyurethane scaffolds using an electrospinning technique and used them for culturing AF-derived-stem/progenitor cells （AFSCs）. Random fibrous scaffolds, also prepared via electrospinningy were used as a control. We compared the morphology, proliferation, gene expression and matrix production of AFSCs on aligned scaffolds and random scaffolds. There was no apparent difference in the attachment or proliferation of cells cultured on aligned scaffolds and random scaffolds. However, compared to cells on random scaffolds, the AFSCs on aligned scaffolds were more elongated and better aligned, and they exhibited higher gene expression and matrix production of coUagen-I and aggrecan. The gene expression and protein production of coUagen-II did not appear to differ between the two groups. Together, these findings indicate that aligned fibrous scaffolds may provide a favourable microenvironment for the differentiation of AFSCs into cells similar to outer AF cells, which predominantly produce collagen-I matrix.展开更多
The reasonable marketing orientation is related to the survival and the development of the enterprises. According to the disadvantages and superiority of the middle and small sized enterprises of our country, this pap...The reasonable marketing orientation is related to the survival and the development of the enterprises. According to the disadvantages and superiority of the middle and small sized enterprises of our country, this paper analyzes the new thought about the marketing orientation from the aspects of marketing product orientation, marketing way orientation, marketing target orientation, marketing strategy orientation and marketing idea orientation. It has great theoretical and practical significance.展开更多
The necessi ty of product oriented integrated computer aided process planning (CAPP) is analy zed, and the system architecture and the main functions are described in detail. The key issues of the system, such as the ...The necessi ty of product oriented integrated computer aided process planning (CAPP) is analy zed, and the system architecture and the main functions are described in detail. The key issues of the system, such as the product oriented process planning me thod, the human machine cooperation in process planning, the generation of manu facture bill of material (MBOM) and the dynamic data exchanging technology betwe en CAPP and enterprise resource planning (ERP), are discussed. The CAPP system h as been applied at a CIMS environment corporation successfully.展开更多
Insect pests threaten food security and food safety through losses caused to crops and livestock,the transmission of diseases to livestock and humans,and diminished trade-oriented production systems.The Food and Agric...Insect pests threaten food security and food safety through losses caused to crops and livestock,the transmission of diseases to livestock and humans,and diminished trade-oriented production systems.The Food and Agricultural Organization of the United Nations(FAO)estimates that insect pests reduce total agricultural output by 30%to 40%.The FAO further indicates that insect pest-derived losses are likely to increase with agricultural intensification and the globalization and international trade of agricultural commodities,the latter of which results in an increased movement of important invasive insect species.Finally,climate change may allow insect pests to invade and prosper in previously inhospitable habitats.展开更多
基金supported by the National Natural Science Foundation of China (81171479, 51303120, 81471790)the China Postdoctoral Science Foundation (2012M521121)+2 种基金the Natural Science Foundation of Jiangsu Province (BK20130335)the Jiangsu Provincial Special Program of Medical Science (BL2012004)the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘Annulus fibrosus （AF） tissue engineering has recently received increasing attention as a treatment for intervertebral disc 0VD） degeneration; however, such engineering remains challenging because of the remarkable complexity of AF tissue. In order to engineer a functional AF replacement, the fabrication of cell-scaffold constructs that mimic the cellular, biochemical and structural features of native AF tissue is critical. In this study, we fabricated aligned fibroua polyurethane scaffolds using an electrospinning technique and used them for culturing AF-derived-stem/progenitor cells （AFSCs）. Random fibrous scaffolds, also prepared via electrospinningy were used as a control. We compared the morphology, proliferation, gene expression and matrix production of AFSCs on aligned scaffolds and random scaffolds. There was no apparent difference in the attachment or proliferation of cells cultured on aligned scaffolds and random scaffolds. However, compared to cells on random scaffolds, the AFSCs on aligned scaffolds were more elongated and better aligned, and they exhibited higher gene expression and matrix production of coUagen-I and aggrecan. The gene expression and protein production of coUagen-II did not appear to differ between the two groups. Together, these findings indicate that aligned fibrous scaffolds may provide a favourable microenvironment for the differentiation of AFSCs into cells similar to outer AF cells, which predominantly produce collagen-I matrix.
文摘The reasonable marketing orientation is related to the survival and the development of the enterprises. According to the disadvantages and superiority of the middle and small sized enterprises of our country, this paper analyzes the new thought about the marketing orientation from the aspects of marketing product orientation, marketing way orientation, marketing target orientation, marketing strategy orientation and marketing idea orientation. It has great theoretical and practical significance.
文摘The necessi ty of product oriented integrated computer aided process planning (CAPP) is analy zed, and the system architecture and the main functions are described in detail. The key issues of the system, such as the product oriented process planning me thod, the human machine cooperation in process planning, the generation of manu facture bill of material (MBOM) and the dynamic data exchanging technology betwe en CAPP and enterprise resource planning (ERP), are discussed. The CAPP system h as been applied at a CIMS environment corporation successfully.
文摘Insect pests threaten food security and food safety through losses caused to crops and livestock,the transmission of diseases to livestock and humans,and diminished trade-oriented production systems.The Food and Agricultural Organization of the United Nations(FAO)estimates that insect pests reduce total agricultural output by 30%to 40%.The FAO further indicates that insect pest-derived losses are likely to increase with agricultural intensification and the globalization and international trade of agricultural commodities,the latter of which results in an increased movement of important invasive insect species.Finally,climate change may allow insect pests to invade and prosper in previously inhospitable habitats.
