The increased number of annotated bacterial genomes provides a vast resource for genome mining.Several bacterial natural products with epoxide groups have been identified as pre-mRNA spliceosome inhibitors and antitum...The increased number of annotated bacterial genomes provides a vast resource for genome mining.Several bacterial natural products with epoxide groups have been identified as pre-mRNA spliceosome inhibitors and antitumor compounds through genome mining.These epoxide-containing natural products feature a common biosynthetic characteristic that cytochrome P450s(CYPs)and its patterns such as epoxidases are employed in the tailoring reactions.The tailoring enzyme patterns are essential to both biological activities and structural diversity of natural products,and can be used for enzyme pattern-based genome mining.Recent development of direct cloning,heterologous expression,manipulation of the biosynthetic pathways and the CRISPR-CAS9 system have provided molecular biology tools to turn on or pull out nascent biosynthetic gene clusters to generate a microbial natural product library.This review focuses on a library of epoxide-containing natural products and their associated CYPs,with the intention to provide strategies on diversifying the structures of CYP-catalyzed bioactive natural products.It is conceivable that a library of diversified bioactive natural products will be created by pattern-based genome mining,direct cloning and heterologous expression as well as the genomic manipulation.展开更多
This paper conceptualizes a framework for bridging the BIM （building information modelling）-specifications divide through augmenting objects within BIM with specification parameters derived from a product library. W...This paper conceptualizes a framework for bridging the BIM （building information modelling）-specifications divide through augmenting objects within BIM with specification parameters derived from a product library. We demonstrate how model information, enriched with data at various LODs （levels of development）, can evolve simultaneously with design and construction using different representation of a window object embedded in a wall as lifeeyele phase exemplars at different levels of granularity. The conceptual standpoint is informed by the need for exploring a methodological approach which extends beyond current limitations of current modelling platforms in enhancing the information content of BIM models. Therefore, this work demonstrates that BIM objects can be augmented with construction specification parameters leveraging product libraries.展开更多
基金a US National Institute of Health/National Cancer Institute grant(R01 CA152212)awarded to Prof。
文摘The increased number of annotated bacterial genomes provides a vast resource for genome mining.Several bacterial natural products with epoxide groups have been identified as pre-mRNA spliceosome inhibitors and antitumor compounds through genome mining.These epoxide-containing natural products feature a common biosynthetic characteristic that cytochrome P450s(CYPs)and its patterns such as epoxidases are employed in the tailoring reactions.The tailoring enzyme patterns are essential to both biological activities and structural diversity of natural products,and can be used for enzyme pattern-based genome mining.Recent development of direct cloning,heterologous expression,manipulation of the biosynthetic pathways and the CRISPR-CAS9 system have provided molecular biology tools to turn on or pull out nascent biosynthetic gene clusters to generate a microbial natural product library.This review focuses on a library of epoxide-containing natural products and their associated CYPs,with the intention to provide strategies on diversifying the structures of CYP-catalyzed bioactive natural products.It is conceivable that a library of diversified bioactive natural products will be created by pattern-based genome mining,direct cloning and heterologous expression as well as the genomic manipulation.
文摘This paper conceptualizes a framework for bridging the BIM （building information modelling）-specifications divide through augmenting objects within BIM with specification parameters derived from a product library. We demonstrate how model information, enriched with data at various LODs （levels of development）, can evolve simultaneously with design and construction using different representation of a window object embedded in a wall as lifeeyele phase exemplars at different levels of granularity. The conceptual standpoint is informed by the need for exploring a methodological approach which extends beyond current limitations of current modelling platforms in enhancing the information content of BIM models. Therefore, this work demonstrates that BIM objects can be augmented with construction specification parameters leveraging product libraries.
