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Prochloraz采后处理对苹果贮藏期病害的控制 被引量:2
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作者 盛占武 鄯晋晓 +2 位作者 毕阳 葛永红 孙志高 《保鲜与加工》 CAS 2006年第4期30-32,共3页
经Prochloraz处理对苹果采后青霉病(peniciliumexpansum)的抑制效果进行了研究。结果表明,Prochloraz对P.expansum具有明显的抑制作用,浸泡处理可明显地降低损伤接种P.expansum的病斑直径,其中以500mg/LProchloraz在40℃浸泡处理对降低... 经Prochloraz处理对苹果采后青霉病(peniciliumexpansum)的抑制效果进行了研究。结果表明,Prochloraz对P.expansum具有明显的抑制作用,浸泡处理可明显地降低损伤接种P.expansum的病斑直径,其中以500mg/LProchloraz在40℃浸泡处理对降低损伤接种P.expansum的病斑直径效果最佳;不同处理时间对病斑直径也有一定的影响,以500mg/LProchloraz处理2min的抑制效果最佳。 展开更多
关键词 苹果 prochloraz 青霉菌
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Control Effect of 35% Thiamethoxam-prochloraz FS to Plant Diseases and Insect Pests at Rice Seedling Stage and Safety Evaluation 被引量:1
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作者 姚善良 李红阳 +3 位作者 周加春 张俊喜 顾慧玲 孙星星 《Agricultural Science & Technology》 CAS 2015年第12期2612-2614,2748,共4页
The prevention and control effect of 35% thiamethoxam-prochloraz FS to rice thrips and rice bakanae disease and its influence on the quality of rice seedlings were studied. The results show that when 100 kg of rice se... The prevention and control effect of 35% thiamethoxam-prochloraz FS to rice thrips and rice bakanae disease and its influence on the quality of rice seedlings were studied. The results show that when 100 kg of rice seeds were coated with 200 g of 35% thiamethoxam-prochloraz FS,the control effects to rice thrips were 93.0% and 84.7% 15 and 20 days after sowing, and the control effect to rice bakanae disease was 90.8% 30 days after sowing. As 100 kg of rice seeds were coated with 250 g of 35% thiamethoxam-prochloraz FS, the control effects to rice thrips were 98.2% and 94.9% 15 and 20 days after sowing, and the control ef- fect to rice bakanae disease was 94.6% 30 days after sowing. 35% thiamethoxam- prochloraz FS is safe to rice seedlings and can effectively promote the growth of rice seedlings. 展开更多
关键词 THIAMETHOXAM prochloraz Seed coating Rice thrips Rice bakanae disease PREVENTION
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Sensitivity of Fusarium graminearum to Carbendazim and Prochloraz 被引量:2
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作者 Ma Dongfang Huang Shi +5 位作者 Zhang Peng Sun Cai Huang Wendi Li Guangjun Zhang Changqing Fang Zhengwu 《Plant Diseases and Pests》 CAS 2017年第1期21-23,共3页
[ Objective] The paper was to understand the resistance of Fusarium graminearum to carbendazim and prochloraz in the main wheat producing areas in Hubei Province. [ Method] Totally 350 F. graminearum strains isolated ... [ Objective] The paper was to understand the resistance of Fusarium graminearum to carbendazim and prochloraz in the main wheat producing areas in Hubei Province. [ Method] Totally 350 F. graminearum strains isolated in 2014 were determined to clear their sensitivity to carbendazim and prochloraz. [ Result] ALl isolates were sensitive to carbendazim and procidoraz in Hubei Province, and carbendazim was still valuable in controlling Fusarium head blight. The control effect of prochloraz was better than that of carbendazim, and the combination of prochloraz and carbendazim at the proportion of 1:7 received the best control effect. [ Conclusion ] Combination of chemical agents will occupy a more and more important position in future prevention and control against Fusarium head blight. 展开更多
关键词 Fusarium graminearum CARBENDAZIM prochloraz Sensitivity Fungicide resistance
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Effect of Compound Treatment with Prochloraz and Chitosan on the Physicochemical Indexes of Refrigerated Citrus Gonggan 被引量:1
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作者 Dongdi XIE Dingli LU 《Agricultural Biotechnology》 CAS 2019年第3期98-101,共4页
[Objectives]This study was conducted to investigate the synergized preservation effect of different concentrations of prochloraz and chitosan on citrus Gonggan,so as to prolong the sales period of Gonggan.[Methods]&qu... [Objectives]This study was conducted to investigate the synergized preservation effect of different concentrations of prochloraz and chitosan on citrus Gonggan,so as to prolong the sales period of Gonggan.[Methods]"Zhongshan Gonggan" was used as a material.The Gonggan fruit was soaked with prochloraz (250,300 and 350 mg/L) and then coated with different concentrations of chitosan (1.0% and 1.5%),respectively.The good fruit rate,weight loss rate and titratable acid (TA),reducing sugar,total soluble solids (TSS) and Vc contents were used as evaluation indexes to investigate the preservation effect of Gonggan during cold storage.[Results]The treatment of prochloraz plus chitosan can significantly prolong the shelf life of Gonggan as it can inhibit the decline of good fruit rate,reduce the weight loss rate,and slow down the degradation and loss of titratable acid,reducing sugar,soluble solids and Vc.The 350 mg/L prochloraz+1.0% chitosan treatment was superior to other treatments.[Conclusions]This study provides a theoretical basis for better prolonging the storage period of Gonggan. 展开更多
关键词 ZHONGSHAN GONGGAN CHITOSAN prochloraz Cold storage PHYSICOCHEMICAL indexes
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Residue and Safety Use of 45% Prochloraz EW in Ginger 被引量:1
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作者 Feng Yizhi Han Jifeng +5 位作者 Qi Xiaoxue Wang Xiaoyu Pan Jinju Jin Jie Liang Lin Zuo Bojun 《Plant Diseases and Pests》 CAS 2019年第5期28-30,共3页
[Objective]The paper was to evaluate the safety of 45%prochloraz EW in ginger,and to study the residue and degradation of 45%prochloraz EW in ginger.[Method]45%Prochloraz EW was sprayed once at the dose of 455.625 g a... [Objective]The paper was to evaluate the safety of 45%prochloraz EW in ginger,and to study the residue and degradation of 45%prochloraz EW in ginger.[Method]45%Prochloraz EW was sprayed once at the dose of 455.625 g a.i./ha in dynamic degradation test,and ginger plants were collected at 2 h,1,3,5,7,14,21,28,35 and 42 d post spraying,respectively.45%Prochloraz EW was sprayed at the doses of 303.75 and 455.625 g a.i./ha for 2-3 times with an interval of 7 d in final residue test,and ginger plants were collected at 7,14 and 21 d post spraying,respectively.The content of prochloraz was quantitatively analyzed by gas chromatography.[Result]Dynamic degradation test demonstrated that prochloraz degraded quickly in plants,and the half-life in Shandong and Anhui were 5.8 and 7.9 d,respectively.The final residue of prochloraz in ginger samples was<0.01-0.145 mg/kg,lower than the maximum residue limit of prochloraz in ginger formulated by European Union(0.2 mg/kg).[Conclusion]Prochloraz should be sprayed at the recommended dose of 202.5-303.75 g a.i./ha for prevention and control of anthracnose on ginger.The agent should be sprayed twice in the early stage of incidence,with an interval of 7 d,and the safety interval of 14 d was safe. 展开更多
关键词 prochloraz GINGER RESIDUE SAFETY USE
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Preservation Effect of Prochloraz-manganese Chloride Complex on Postharvest ‘Shatangju'( Citrus reticulata)Fruits 被引量:2
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作者 Xu Chengxiang Zheng Fuqing +1 位作者 Ma Yanping Zhang Xujia 《Plant Diseases and Pests》 CAS 2016年第5期32-35,共4页
[ Objective ] The paper was to solve the weakness of heavy postharvest diseases and non-endurable storage of ' Shatangju', to improve the preservation effect of ' Shatangju'. [ Method ] Fruits were dipped in diffe... [ Objective ] The paper was to solve the weakness of heavy postharvest diseases and non-endurable storage of ' Shatangju', to improve the preservation effect of ' Shatangju'. [ Method ] Fruits were dipped in different concentrations of procbloraz-manganesc chloride complex solutions. The postharvest weight loss rate, soluble solids content and decay rate of fruits were determined, and the safety of chemical agents was observed and evaluated. [ Result ] Prochloraz-manganese chloride complex had significant preservation effect on postharvest ' Shatangju' fruits, and the appropriate concentration was 0.15% -0.20% (effective content) ; the decay rates of fruits stored at room temperature for 15, 30, 45, 60 and 90 d were 0, 5%, 〈 10%, 〈 10% and 〈 30% ; there was no specific changes in smell and flavor of non-decayed fruits. [ Conclusion ] Prochloraz-manganese chloride complex solution had significant preservation effect on ' Shatangju' by inhibiting and reducing the growth and development of pathogenic bacteria, and the effect was much better under refrigeration condition. 展开更多
关键词 Shatangju' prochloraz-manganese chloride complex Postharvest diseases PRESERVATION
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A smart dual-responsive nanoplatform for delivery of prochloraz for the control of rice blast disease 被引量:1
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作者 Zhaoyang Zhang Donglin Li +7 位作者 Chang Yu Jiaqing Li Dan Sun Jiayin Wang Mohamed Mmby Jianhong Li Hong You Shun He 《Advanced Agrochem》 2024年第4期328-336,共9页
Nano-controlled release formulations present a promising strategy to mitigate pesticide losses and enhance efficiency.In this study,a pH and GSH-responsive nanoplatform using mesoporous organosilica nanoparticles(MONs... Nano-controlled release formulations present a promising strategy to mitigate pesticide losses and enhance efficiency.In this study,a pH and GSH-responsive nanoplatform using mesoporous organosilica nanoparticles(MONs)as a carrier and poly(tannic acid)(PTA)as capping agent was established for controlling prochloraz(Pro)release.The obtained Pro@MON@PTA was characterized by transmission electron microscopy(TEM),scanning electron microscopy(SEM),Fourier transform infrared spectroscopy(FTIR),thermogravimetric analysis(TGA).The results indicate the successful preparation of Pro@MON@PTA nanoparticles,featuring uniform particle size(190 nm),excellent dispersibility,and a prochloraz loading efficiency of 17.2%.Evaluation of contact angle and adhesion work demonstrated superior adhesion of MON@PTA to rice leaves compared to MON.Controlled release studies revealed dual-responsive release properties of Pro@MON@PTA to acid and GSH.Additionally,photostability testing indicated effective ultraviolet light shielding by the carrier,reducing prochloraz degradation under irradiation.Bioassay results indicated equivalent fungicidal activity against Magnaporthe oryzae between Pro@MON@PTA and prochloraz technical and prochloraz EW after a 7-day treatment.However,in vivo experiments demonstrated that Pro@MON@PTA exhibited superior control efficacy compared to prochloraz EW.These findings suggested that MON@PTA holds significant potential for plant disease management. 展开更多
关键词 prochloraz Mesoporous organosilica nanoparticles Controlled-released formulation Magnaporthe oryzae Biological activity
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Inhibition Effect and Synergistic Effect of Prochloraz and Tebuconazole and Their Mixture on Fusarium graminearum
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作者 Zhang Xing Zhang Lin +5 位作者 He Yiqin Lu Chen Fang Zhengwu Zhao Mingmin Zhu Yongxing Ma Dongfang 《Plant Diseases and Pests》 CAS 2017年第6期28-30,共3页
Wheat scab caused by Fusarium graminearum is increasingly severe in the middle and lower reaches of the Yangtze River region. With long-term use of single chemical pesticide,the drug resistance of F. graminearum is gr... Wheat scab caused by Fusarium graminearum is increasingly severe in the middle and lower reaches of the Yangtze River region. With long-term use of single chemical pesticide,the drug resistance of F. graminearum is growing day by day. In order to reveal the inhibition effect and synergistic effect of prochloraz and tebuconazole and their mixture on F. graminearum,new compound agents for controlling wheat scab were developed. The inhibition effect and synergistic effect of prochloraz and tebuconazole mixed at seven different ratios on F. graminearum were studied. The results showed that the efficiency of the mixture was the best when the proportion of prochloraz and tebuconazole was 1:7; the EC50 was 0.145 36 and the synergistic coefficient was 1.853 9. The results provide a theoretical basis for development of new compound agents and effective control of wheat scab. 展开更多
关键词 FUSARIUM GRAMINEARUM prochloraz TEBUCONAZOLE Compound agent Sensitivity
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Preservation Effect of Prochloraz-manganese Chloride Complex on ‘Gonggan’ (Citrus reticulata) Fruits
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作者 Xu Chengxiang Ma Yanping Liu Haobin 《Plant Diseases and Pests》 CAS 2017年第6期45-48,共4页
‘Gonggan’( Citrus reticulata) is a famous,high-quality and special citrus cultivar in the Xijiang River valley,Guangdong Province. However,it easily suffers from postharvest diseases,and thus is not tolerant to stor... ‘Gonggan’( Citrus reticulata) is a famous,high-quality and special citrus cultivar in the Xijiang River valley,Guangdong Province. However,it easily suffers from postharvest diseases,and thus is not tolerant to storage. Based on the preliminary test results,prochloraz-manganese chloride complex was selected as the disinfectant,and prepared into different concentrations for carrying out preservation test by dipping fruits. The fruit weight loss,soluble solids content and decay rate were analyzed,and the application security and sensory evaluation for fruit freshness and flavor were observed. The results showed that dipping fruits with prochloraz-manganese chloride complex solution had significant preservation effect on‘Gonggan’fruits in postharvest storage; the suitable concentration was 2. 0-3. 0 g/L,and the decay rates of fruits stored at room temperature for 30,60 and 90 d were below 5%,10% and 15%,respectively. Plate inoculation test further revealed that the pathogen of‘Gonggan’was sensitive to prochloraz-manganese chloride complex,and the concentration of 1. 0 g/L was enough to produce significant antimicrobial effect. Other technical links related to postharvest preservation of‘Gonggan’with prochloraz-manganese chloride complex were discussed. 展开更多
关键词 'Gonggan’ prochloraz-manganese chloride complex POSTHARVEST diseases PRESERVATION
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Application of Prochloraz-nano-titanium Dioxide Complex Fresh-keeping Agent in Refrigeration of Dioscorea alata Lirm. sp. 被引量:1
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作者 Dongdi XIE Yanpei WEI +1 位作者 Xiaoping WEI Wanwei WU 《Asian Agricultural Research》 2018年第10期77-81,共5页
Prochloraz diluents( 450 ×,475 ×,500 ×) were added with different concentrations of nano-titanium dioxide( Ti O2)( 0. 8%,1. 0%) respectively to prepare complex fresh-keeping agents,which were applied in... Prochloraz diluents( 450 ×,475 ×,500 ×) were added with different concentrations of nano-titanium dioxide( Ti O2)( 0. 8%,1. 0%) respectively to prepare complex fresh-keeping agents,which were applied in the refrigeration of Dioscorea alata Lirm. sp. With rot index,weight loss rate,browning degree( BD),total soluble solids( TSS) content,malondialdehyde( MDA) content,polyphenol oxidase( PPO) activity and peroxidase( POD) activity,DPPH· scavenging rate as evaluation indexes,the preservation effect of the prepared freshkeeping agents on D. alata during the cold storage period was analyzed. The results showed that prochloraz added with nano-titanium dioxide significantly prolonged the storage life,reduced the rot rate and weight loss rate,and slowed the loss of pulp of D. alata. The preservation effect of complex fresh-keeping agent of prochloraz diluent( 475 ×) and nano-titanium dioxide( 1. 0%) was better than that of other treatments. 展开更多
关键词 纳米钛 农业 科技创新 发展现状
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Rice husk-derived lignin-coated mesoporous silica nanoparticles for fungicide delivery: Enhancing leaf adhesion/distribution and translocation of prochloraz in plants
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作者 Andreia Abadia Borges Carneiro Samilla de Almeida +6 位作者 Mansi Goyal Soumitra Patekar Carlos Horacio Luna-Flores Adrian Baker Tal Cooper Morteza Hassanpour Zhanying Zhang 《Nano Research》 2025年第10期1160-1172,共13页
In this study,an innovative and sustainable nanocarrier system that was made of rice husk-derived lignin and biosilica was developed for smart delivery of prochloraz(Pro),a widely used fungicide.Mesoporous silica nano... In this study,an innovative and sustainable nanocarrier system that was made of rice husk-derived lignin and biosilica was developed for smart delivery of prochloraz(Pro),a widely used fungicide.Mesoporous silica nanoparticles(MSNPs)were first synthesized from rice husk ash using hexadecyltrimethylammonium bromide(CTAB)as the structural agent through an acid precipitation method.The resulting MSNPs were further functionalized with amino groups(MSNPs-NH_(2))to enhance the loading of Pro onto the nanocarrier,likely due to a combination of hydrogen bonds,π-π interactions,and electrostatic interactions(Pro@MSNPs-NH_(2)).Lignin,recovered after an acid-catalyzed organosolv pretreatment of rice husk,was coated to Pro@MSNPs-NH_(2)(Pro@MSNPs-NH_(2)-lignin).The rice husk-derived nanocarrier system was evaluated in terms of its capacity to distribute,adhere to,and translocate within model plants(cotton,soybean,and tomato)as well as its efficacy against Fusarium oxysporum.The results revealed that lignin coating imparted hydrophobicity to hydrophilic MSNPs-NH_(2),enhancing their foliar distribution and adherence as well as washout resistance onto hydrophobic plant leaves.Moreover,translocation of Pro was improved because of the nano-scale size of MSNPs,overcoming the challenges in poor absorption by plant leaves.Bioactivity trial results showed that the Pro@MSNPs-NH_(2)-lignin delivery system exhibited effective fungicidal performance,which led to 80.5%of fungal growth inhibition at 0.125 mg Pro/mL on Day 4,comparable to 75.0%of growth inhibition with the use of pure prochloraz at the same loading.Overall,this study demonstrated the significant potential of rice husk-derived sustainable nanocarriers for efficient delivery of agrochemicals,supporting the development of sustainable and resilient agriculture. 展开更多
关键词 rice husk LIGNIN mesoporous silica nanoparticles prochloraz delivery
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氟唑菌酰羟胺复配咪鲜胺对油菜菌核病的防治效果
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作者 李勇峰 何志明 +5 位作者 叶铁军 唐巧弟 刘颖 刘帅 王璐 何花 《安徽农学通报》 2026年第1期68-70,共3页
为验证氟唑菌酰羟胺复配咪鲜胺对油菜菌核病的防治效果与应用效益,本文以邡油777为试验对象,设置20%氟唑菌酰羟胺悬浮剂(SC)750 mL/hm^(2)+25%咪鲜胺乳油(EC)750 mL/hm^(2)(处理1)、40%多菌灵·福美双可湿性粉剂(WP)1200 g/hm^(2)(... 为验证氟唑菌酰羟胺复配咪鲜胺对油菜菌核病的防治效果与应用效益,本文以邡油777为试验对象,设置20%氟唑菌酰羟胺悬浮剂(SC)750 mL/hm^(2)+25%咪鲜胺乳油(EC)750 mL/hm^(2)(处理1)、40%多菌灵·福美双可湿性粉剂(WP)1200 g/hm^(2)(处理2)和空白对照(CK)3个处理,在当地田间开展小区试验,调查各处理的安全性,并计算防治效果和经济效益。结果表明,试验剂量下,各药剂处理对油菜生长安全;处理1的病株防效、病指防效分别为87.85%、91.39%,高于处理2,与处理2差异均具有统计学意义(P<0.01);处理1较处理2增产575.4 kg/hm^(2),较CK增产875.85 kg/hm^(2);处理1(15277.98元/hm^(2))和处理2(11414.94元/hm^(2))的收益均高于CK。综合表明,推荐在研究区施用20%氟唑菌酰羟胺SC 750 mL/hm^(2)复配25%咪鲜胺EC 750 mL/hm^(2)(处理1)防治油菜菌核病。 展开更多
关键词 氟唑菌酰羟胺 咪鲜胺 油菜菌核病 防治效果
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咪鲜胺及其复配剂对河南省禾谷镰孢的抑菌活性及对小麦赤霉病的室内防效 被引量:3
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作者 崔永源 高续恒 +2 位作者 钱乐 姜佳 刘圣明 《植物保护》 北大核心 2025年第1期262-270,310,共10页
为确定咪鲜胺对河南省禾谷镰孢Fusarium graminearum的抑菌活性,测定了其对禾谷镰孢不同发育阶段的毒力。结果表明:咪鲜胺对孢子萌发、分生孢子产生、芽管伸长和菌丝生长的有效抑制中浓度(EC _(50))分别为3.8689、0.3156、0.0631μg/mL... 为确定咪鲜胺对河南省禾谷镰孢Fusarium graminearum的抑菌活性,测定了其对禾谷镰孢不同发育阶段的毒力。结果表明:咪鲜胺对孢子萌发、分生孢子产生、芽管伸长和菌丝生长的有效抑制中浓度(EC _(50))分别为3.8689、0.3156、0.0631μg/mL和0.0223μg/mL。对2019年、2021年和2022年河南省6个地市随机选取的210株禾谷镰孢对咪鲜胺的敏感性进行测定。结果显示:咪鲜胺对供试禾谷镰孢菌丝生长的EC _(50)范围为0.0018~0.0572μg/mL,平均EC _(50)为(0.0233±0.0086)μg/mL。敏感性频率分布呈单峰曲线,符合正态分布。测定了咪鲜胺原药与叶菌唑、氰烯菌酯、氟啶胺、咯菌腈4种杀菌剂原药之间分别按照质量比1∶5、1∶3、1∶1、3∶1和5∶1配比的复配剂对禾谷镰孢的联合毒力。结果显示:增效系数(SR)范围为0.54~3.94。不同组合、不同比例的复配剂均表现为相加作用或增效作用,其中咪鲜胺∶氟啶胺按质量比3∶1复配时,增效系数最大。表明咪鲜胺可以与叶菌唑、氰烯菌酯、氟啶胺、咯菌腈等杀菌剂复配使用。防效试验结果表明,45%咪鲜胺水乳剂与50%氟啶胺悬浮剂按照3∶1比例复配以160μg/mL浓度喷施保护效果达到100%。本试验结果可以为小麦赤霉病的治理提供依据。 展开更多
关键词 禾谷镰孢 咪鲜胺 复配剂 敏感性 杀菌活性
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柑橘采后分选中咪鲜胺和抑霉唑浓度检测 被引量:1
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作者 张家宁 龙复涵 +6 位作者 杨中华 李亚荣 谢合平 林杨 郑文艳 于晓 付艳苹 《华中农业大学学报》 北大核心 2025年第2期221-227,共7页
为了科学规范咪鲜胺和抑霉唑在柑橘果实保鲜处理中的使用,基于HPLC建立一种同时检测较高质量浓度条件下咪鲜胺和抑霉唑的方法。结果显示,在5~300μg/mL范围内,咪鲜胺和抑霉唑线性关系良好,该方法灵敏度和准确度高。温州蜜柑采后分选线上... 为了科学规范咪鲜胺和抑霉唑在柑橘果实保鲜处理中的使用,基于HPLC建立一种同时检测较高质量浓度条件下咪鲜胺和抑霉唑的方法。结果显示,在5~300μg/mL范围内,咪鲜胺和抑霉唑线性关系良好,该方法灵敏度和准确度高。温州蜜柑采后分选线上,室温条件下咪鲜胺稳定,抑霉唑降解率高,工作6 h处理温州蜜柑15 t,咪鲜胺和抑霉唑质量浓度分别降低98.6、290.3μg/mL。结果表明,该方法可以应用于柑橘采后处理过程中咪鲜胺和抑霉唑的质量浓度检测,有利于提高杀菌剂使用效率及降低生产成本。 展开更多
关键词 柑橘采后病害 咪鲜胺 抑霉唑 高效液相色谱法
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番茄灰霉病菌对咪鲜胺的抗性检测及风险评估
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作者 任潇妍 贾仡伟 +5 位作者 王潞伟 李甜爽 刘海霞 姚艳平 王春伟 王美琴 《园艺学报》 北大核心 2025年第7期1915-1925,共11页
为了明确番茄灰霉病菌对咪鲜胺的抗性水平及抗性风险,采用菌丝生长速率法建立了敏感基线,并检测了山西省不同地区番茄灰霉病菌对咪鲜胺的抗性水平;采用药剂驯化和紫外诱导探究了番茄灰霉病菌对咪鲜胺的抗性风险,并对抗性突变体的遗传稳... 为了明确番茄灰霉病菌对咪鲜胺的抗性水平及抗性风险,采用菌丝生长速率法建立了敏感基线,并检测了山西省不同地区番茄灰霉病菌对咪鲜胺的抗性水平;采用药剂驯化和紫外诱导探究了番茄灰霉病菌对咪鲜胺的抗性风险,并对抗性突变体的遗传稳定性及生物学特性进行了研究,测定了咪鲜胺与常用药剂之间的交互抗性。结果显示:从未使用过咪鲜胺的地区分离的89株番茄灰霉病菌,建立的敏感基线为(0.1297±0.0765)μg·mL^(-1);在山西省各地分离到116株番茄灰霉病菌,检测到6株低抗菌株,抗性频率为5.17%。通过药剂驯化和紫外诱导各获得3株中抗菌株和1株低抗菌株,转接8代后抗性可稳定遗传;抗性突变体的生物学特性低于亲本菌株。咪鲜胺与戊唑醇、氟吡菌酰胺、咯菌腈、嘧霉胺、异菌脲和百菌清之间均无交互抗性。总之,山西省番茄灰霉病菌群体对咪鲜胺敏感,抗性水平较低;咪鲜胺对灰霉病菌的抗性菌株不易形成优势菌株,属于低至中等抗性风险;生产上可以交替使用不同作用机理的药剂防治番茄灰霉病,延缓灰霉病菌对咪鲜胺抗性的产生。 展开更多
关键词 番茄 灰霉病菌 咪鲜胺 敏感基线 抗性检测 风险评估
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二氯异氰尿酸钠、咪鲜胺及其代谢物在杨梅贮藏保鲜中的消解动态及安全评价
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作者 高萨 史冉冉 +7 位作者 滕玲 王炜涛 杜雨婷 罗婷 杨桂玲 王新全 丁伟 王豆 《食品安全质量检测学报》 2025年第12期97-105,共9页
目的 评估二氯异氰尿酸钠(sodium dichloroisocyanurate,DCCNa)、咪鲜胺及其代谢物在杨梅中的残留消解动态及其膳食摄入风险。方法 采用液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS/MS)对常温和冷藏贮... 目的 评估二氯异氰尿酸钠(sodium dichloroisocyanurate,DCCNa)、咪鲜胺及其代谢物在杨梅中的残留消解动态及其膳食摄入风险。方法 采用液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS/MS)对常温和冷藏贮藏条件下杨梅中DCCNa、咪鲜胺及其代谢物2,4,6-三氯苯酚的残留动态进行定量分析,并评估其膳食摄入风险。结果 DCCNa、咪鲜胺浸果的浓度与残留量之间存在正相关,即浸果浓度越高,初始残留量越高。两种农药在杨梅中的消解动态均符合一级动力学方程,其中DCCNa在常温与冷藏条件下的半衰期分别为0.5~0.9 d和0.8~1.1 d,而咪鲜胺分别为1.1~1.3 d和1.5~2.1 d,且咪鲜胺的代谢物2,4,6-三氯苯酚的含量在贮藏期间呈现增加趋势。膳食风险评估结果显示,用质量浓度为15mg/L、60mg/L的DCCNa和45 mg/L、180 mg/L的咪鲜胺溶液分别浸泡杨梅保鲜,最高残留水平下杨梅中两种农药的急性膳食摄入风险均在可接受的范围内;市售杨梅中DCCNa和咪鲜胺的残留水平低于实验处理组最终残留量,且急性膳食摄入风险值小于100%,处于可控范围内。结论 本研究通过阐明DCCNa、咪鲜胺及其代谢物在杨梅贮藏保鲜过程中的降解规律,为杀菌保鲜剂的科学规范使用和质量安全监管提供理论依据。 展开更多
关键词 二氯异氰尿酸钠 咪鲜胺 2 4 6-三氯苯酚 杨梅 贮藏 保鲜 消解动态 膳食风险评估
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茶树轮斑病病菌防治药剂筛选及杀菌机制初步研究
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作者 汤旭 杨森 +1 位作者 杨留勇 薛伟 《广东农业科学》 2025年第11期101-110,共10页
【目的】研究茶树轮斑病病原菌棕榈拟盘多毛孢(Pestalotiopsis trachicarpicola,简写:P.trach.)的致病特性,筛选对该病原菌高效低毒的备选杀菌剂,明确核心药剂的初步作用机制,为茶园针对该病害的季节性精准用药与药剂轮换提供科学依据... 【目的】研究茶树轮斑病病原菌棕榈拟盘多毛孢(Pestalotiopsis trachicarpicola,简写:P.trach.)的致病特性,筛选对该病原菌高效低毒的备选杀菌剂,明确核心药剂的初步作用机制,为茶园针对该病害的季节性精准用药与药剂轮换提供科学依据。【方法】采用菌丝速率生长法,测定18种杀菌剂在100 μg/mL浓度下对P.trach.的抑制活性及半数有效浓度值(EC_(50));评价10 μg/mL咪鲜胺、吡唑醚菌酯和甲基硫菌灵对茶树轮斑病的治疗与保护活性。通过膜外电导率测定法和PI染色法,初步探究咪鲜胺对P.trach.细胞膜的损伤作用机制。【结果】18种杀菌剂对P.trach.的抑菌活性存在显著差异,其中咪鲜胺、异菌脲、吡唑醚菌酯等的抑制率均超过90%,表现出较强抑菌潜力;咪鲜胺、吡唑醚菌酯和甲基硫菌灵对P.trach.的EC_(50)分别为0.17、0.18和1.22 μg/mL,优于阳性对照萎锈灵(12.26 μg/mL)。活体接种法显示,咪鲜胺的保护活性(55.65%)和治疗活性(58.45%)分别较阳性对照萎锈灵(26.09%、18.47%)提高29.56、39.98个百分点,综合防效最优;吡唑醚菌酯保护性较强、甲基硫菌灵治疗性稳定,三者功能互补。咪鲜胺对P.trach.的膜通透性研究表明,4 μg/mL咪鲜胺处理P.trach.8 h后,病原菌膜外绝对电导率(7.85 μS/cm)和相对电导率(71.04%)分别较空白对照CK_(2)(4.81 μS/cm、44.17%)显著提升,表明咪鲜胺可破坏病原菌细胞膜完整性,导致胞内电解质外泄;荧光显微镜观察显示,咪鲜胺对P.trach.细胞膜的损伤程度随处理时间延长呈递增趋势,进一步佐证其对细胞膜的破坏作用。【结论】咪鲜胺、吡唑醚菌酯、甲基硫菌灵分别对P.trach.表现出优异的综合防效、保护活性和治疗活性,且具有低用量优势,为构建基于季节性病害发生规律的防控方案提供了核心药剂轮换组合支撑;其中,咪鲜胺通过破坏P.trach.细胞膜完整性发挥抗菌作用,可作为核心防控备选药剂。 展开更多
关键词 茶树轮斑病 棕榈拟盘多毛孢 杀菌剂筛选 咪鲜胺机制 季节性防控
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咪鲜胺水包油型乳剂生产工艺的优化
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作者 宋文杰 王瑞琪 +4 位作者 闫博宇 郭明欣 李祖迪 乐涛 张超 《农产品加工》 2025年第12期59-64,共6页
采用响应面法优化载药量为15%的咪酰胺水包油型乳剂的生产工艺。以单硬脂酸甘油酯作为油相,以大豆卵磷脂、泊洛沙姆-188和吐温80作为乳化剂,采用响应面分析法优化咪鲜胺水包油型乳剂高压均质生产工艺,以降低粒径、提高包封率。选择乳剂... 采用响应面法优化载药量为15%的咪酰胺水包油型乳剂的生产工艺。以单硬脂酸甘油酯作为油相,以大豆卵磷脂、泊洛沙姆-188和吐温80作为乳化剂,采用响应面分析法优化咪鲜胺水包油型乳剂高压均质生产工艺,以降低粒径、提高包封率。选择乳剂亲水亲油平衡值(Hydrophilic lipophilic balance,HLB值)、均质压力和辅料占比(辅料包括油相和乳化剂)作为因素,以粒径和包封率作为响应值设计了三因素三水平试验。结果表明,辅料占比是影响咪鲜胺水包油型乳剂粒径和包封率的主要因素,提高辅料占比可降低粒径和包封率。模型优化显示,当HLB值为18,辅料占比为19%,均质压力为450 bar时,咪鲜胺水包油型乳剂粒径为19.5μm,包封率为77.83%;验证试验显示,乳剂粒径和包封率与理论值相对偏差分别0.92%和0.17%,该模型准确可靠。 展开更多
关键词 咪鲜胺 水包油型乳剂 响应面法 HLB值 包封率
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冬荪绿霉病菌对咪鲜胺锰盐敏感基线及抗药性监测研究
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作者 刘忠玄 王晶 王芳 《安徽农业科学》 2025年第14期134-137,共4页
为进一步了解冬荪绿霉病菌(绒毛木霉Trichoderma tomentosum)对咪鲜胺锰盐的敏感性和抗药性,采用菌丝生长速率法监测不同来源病菌对咪鲜胺锰盐的敏感性,并建立敏感基线;运用最小抑菌浓度法监测抗性菌株及其发生频率,并比较不同来源菌株... 为进一步了解冬荪绿霉病菌(绒毛木霉Trichoderma tomentosum)对咪鲜胺锰盐的敏感性和抗药性,采用菌丝生长速率法监测不同来源病菌对咪鲜胺锰盐的敏感性,并建立敏感基线;运用最小抑菌浓度法监测抗性菌株及其发生频率,并比较不同来源菌株的抗性水平。结果表明,咪鲜胺锰盐对供试133株病原菌的EC_(50)值为0.0335~7.6941 mg/L,其敏感性频率呈现连续单峰曲线,K-S验证为正态分布,供试菌株未出现明显抗药群体,EC_(50)平均值为3.8572 mg/L,该平均值可作为绒毛木霉对咪鲜胺锰盐的敏感基线;抗性监测中,来自不同地区的103株病原分离物对咪鲜胺锰盐的抗性具有一定差异,抗药性水平多数为敏感或低抗性,二者平均频率之和为85.23%,少数菌株表现为高抗,平均频率为8.93%,说明该药剂对病原菌仍具有较高的防效。该研究结果为咪鲜胺锰盐防治冬荪绿霉病提供一定的理论依据和管理建议。 展开更多
关键词 冬荪 绒毛木霉 咪鲜胺锰盐 抗药性 敏感基线
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高通量消解仪联合气相色谱高效测定山药中咪鲜胺及其代谢物
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作者 吕妍燕 汪浩 +2 位作者 丁白瑜 郝爽 王智 《农药》 北大核心 2025年第8期578-582,共5页
[目的]为了能够大批量、高效、准确的测定山药中咪鲜胺的含量,开发一种高通量消解仪联合气相色谱测定咪鲜胺及其代谢物含量的方法。[方法]采用QuEChERS方法提取、高通量消解仪进行水解处理,提取物净化后利用气相色谱仪(ECD检测器)进行... [目的]为了能够大批量、高效、准确的测定山药中咪鲜胺的含量,开发一种高通量消解仪联合气相色谱测定咪鲜胺及其代谢物含量的方法。[方法]采用QuEChERS方法提取、高通量消解仪进行水解处理,提取物净化后利用气相色谱仪(ECD检测器)进行检测。[结果]标准曲线的范围为50~500μg/L,线性相关系数为0.998,在0.05、0.10、0.25、0.50 mg/kg不同的加标水平下,咪鲜胺的平均回收率范围为91.7%~107.3%,相对标准偏差范围为0.55%~2.0%,符合检测方法确认的技术要求。[结论]该方法准确、快速、安全,适合大批量检测山药中咪鲜胺及其代谢物含量。 展开更多
关键词 山药 咪鲜胺 咪鲜胺代谢物 QuEChERS法 高通量消解仪 气相色谱法
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